Mechanistic Insight Into Traumatic Brain Injury Induced Neuronal Membrane Disruption: Cathepsin B Relocalization And A Neun Negative Cortical Neuron Subpopulation, Martina L. Hernandez

Theses and Dissertations

Traumatic brain injury (TBI) is a mechanical insult to the head that leads to brain damage and, in turn, causes long-term sensory, motor, cognitive, and affective dysfunction. Diffuse pathologies seen following such injury are associated with these life-altering outcomes that impact the daily lives of TBI survivors. The diffuse pathology that this body of work focuses on is neuronal membrane disruption; it is characterized by increased permeability of the neuron’s plasma membrane. Moreover, our lab had previously found that membrane disruption is exacerbated with intracranial pressure (ICP) elevation. We set out to measure the duration of membrane disruption following injury …

Fascism In Sci-Fi: "Mobilizing Passions" In Robert A. Heinlein's Starship Troopers, Alton C. Ayers

Theses and Dissertations

This thesis responds to criticism of Robert A. Heinlein’s Starship Troopers (1959) as a “fascist” novel by further investigating the claim through a close reading of the novel that applies political theory scholarship on fascism. Chapters I and II introduce the novel along with its general reception and controversy. These chapters consider the accusations of “fascism” given to the novel while at the same time understanding that a clear, exact definition of “fascism” has long been grappled with by scholars since the rise of the regimes in Nazi Germany and Fascist Italy. Chapters III and IV apply political theory to …

Supporting Students Who Are Deafblind To Increase Their Access To The General Education Curriculum Through Trained Interveners: A Mixed Methods Study, Ira Padhye

Theses and Dissertations

The purpose of this mixed-methods research study was to identify the educational trends of children and young adults who are deafblind and identify strategies to improve the outcomes through interviews of nationally qualified interveners who have received training in deafblind specific strategies. Secondary data from the 2017-2021 National Center on Deafblindness and interview transcripts were used for data analysis and then integrated to identify the supports students who are deafblind should access. Trends in age of identification, primary eligibility category, school settings and access to state standardized assessments showed a plateau and displayed no significant improvement in educational outcomes. Nationally …

Advanced Modeling And Innovative Transcranial Magnetic Stimulation Coil Designs For The Treatment Of Neurological Disorders, Mohannad Tashli

Theses and Dissertations

Transcranial magnetic stimulation (TMS) is a safe, effective and non-invasive treatment for several mental and psychiatric disorders. TMS is an FDA approved treatment and is commonly applied to patients who do not respond to medications for the treatment of clinical depression, smoking cessation, obsessive-compulsive disorder and migraine. Recently, there has been an increase in the development of electromagnetic neuromodulation techniques targeted at enhancing the effectiveness of TMS devices for the treatment of mental diseases. In TMS stimulation, focality is an important factor which determines the specificity of the pulses induced in different brain tissues. The electromagnetic pulses must be confined …

Activity Levels Of Different Cell Types In An Epileptogenic Model Of Cortical Microgyria: A Cfos Investigation, Peter F. Lamothe

Theses and Dissertations

A large percentage of individuals with intractable epilepsies have an accompanying cortical malformation, the underlying cellular mechanisms of which are not fully understood. In an animal model for one such malformation, polymicrogyria, epileptogenesis occurs most easily in a region of tissue just adjacent to the microgyria termed the paramicrogyral region (PMR). Previous studies implicate somatostatin containing interneurons (SOM) as a potential contributor to this pathology, and show increased excitation of SOM in the PMR. We hypothesis that SOM are more active in the PMR when compared to SOM within the homologous region of the control cortex. In addition to this …

The Cultural Diplomacy Of U.S. Peace Corps: Understanding The Role Of Peace Corps Volunteers As Strategic Geopolitical Assets, Corey B. Miles

Theses and Dissertations

Beginning in the Summer of 1961, the United States government has deployed volunteer humanitarians around the world to serve the mission of U.S. Peace Corps and has since contributed to communities in 143 partner countries while enjoying widespread popularity. Its mission of promoting world peace and friendship is pursued through three operational goals: technical training, cultural exportation, and cultural importation. Since 2016, with the election of U.S. President Donald Trump, and continuing through the Covid-19 pandemic, which produced the first global service interruption in agency history, the value of the Peace Corps service model has been fundamentally challenged. As a …

Well-Being From Home To Work: The Role Of Mindfulness, Alisha Gupta

Theses and Dissertations

Considering the growing spillover of work and home and its impact on well-being, I draw from the Work-Home Resources Model and Attention Restoration Theory to examine the effects of evening recovery experiences at home on mindfulness at work and eudaimonic workplace well-being. Specifically, I focus on two indicators of eudaimonic well-being—one indicator of psychological functioning (work meaningfulness) and one indicator of social functioning (high-quality connections; HQCs). Further, I introduce a boundary condition, distance from work, of the relationship between evening recovery experiences and mindfulness. I suggest that due to alternative work arrangements in today’s workforce (i.e., that permit increased overlap …

Anti-Immigration-Related Stressors And Latine Immigrant Well-Being: The Role Of Family & Community Resilience, Lisa S. Fuentes

Theses and Dissertations

Anti-immigrant rhetoric and policies have continued to adversely impact Deferred Action for Childhood Arrivals (DACA) and Temporary Protection Status (TPS) beneficiaries (Arevalo et al., 2015; Cleary, 2017; Li, 2015; Sangalang et al., 2018). There have been numerous attempts to revoke DACA, TPS, and other policies that temporarily protect immigrant families. Although the number of resilience literature has grown, more strength-based approaches are still needed. Additionally, given that familismo (familism) and collectivism are key cultural values for Latine families (Campos et al., 2019; Corona et al., 2017), it is imperative to explore family and community resilience among Latine immigrants to gain …

Quantitative And Molecular Genetic Analyses Of Brain Structure, Substance Use, And Posttraumatic Stress Phenotypes: Data From Children, Adolescents And Adults, Daniel Bustamante

Theses and Dissertations

Abstract

QUANTITATIVE AND MOLECULAR GENETIC ANALYSES OF BRAIN-IMAGING, SUBSTANCE USE, AND POSTTRAUMATIC STRESS PHENOTYPES: DATA FROM CHILDREN, ADOLESCENTS AND ADULTS

By Daniel Bustamante, B.S., B.A.

Doctoral dissertation submitted in partial fulfillment of the requirements for the degree of Doctor of Philosophy in Integrative Life Sciences – Behavioral and Statistical Genetics at Virginia Commonwealth University.

Virginia Commonwealth University, 2023.

Dissertation directors: Michael C. Neale, Ph.D., Banks Distinguished Professor of Psychiatry & Human and Molecular Genetics; and, Ananda B. Amstadter, Ph.D., Professor of Psychiatry, Psychology, & Human and Molecular Genetics.

Substance use (SU) and posttraumatic stress disorder (PTSD) phenotypes are comorbid, share …

A Theory Of General Relativity, Madeleine Mae Morris

Theses and Dissertations

"A Theory of General Relativity" is a quiet exploration into the poetic and scientific ways we inhabit space and time. This work is a manifestation of the time I spent being my grandmother's caretaker and exists now in the form of an artist book, an exhibition of framed photographs with glass objects, and this written thesis. Throughout those five years, I became consumed by the relationships between Mothers and Daughters, which brought into excruciatingly sharp focus seemingly simple questions like: who are we, what have we done to each other, what do we owe to each other, do I reap …

Treponema Denticola Synthesizes C-Di-Amp And Encodes The Cdaa-Type Diadenylate Cyclase Cdaa, Claire R. O'Brien

Theses and Dissertations

Periodontitis is a form of oral disease characterized by dysbiosis of the oral microbiome, leading to inflammation, bone resorption, and in severe cases, entire tooth loss, affecting 42% of adults in the US. One of the bacteria most associated with periodontal disease progression is Treponema denticola (Td), an oral spirochete which inhabits the mouth in small quantities during health but which can dominate the biofilms that form during periodontal disease. The ability of Td to survive in a disease environment and contribute to the progression of disease requires the use of robust signaling networks. Analysis of Td cultures …

Characterization And Investigation Of Cold Atmospheric Plasma And Its Effects On Cancer Cell Biology, Thomas M. Ritrosky

Theses and Dissertations

Modern cancer treatment uses radiation therapy in over 50% of patient cases. It is an e↵ective way of treating tumors because the mechanisms of cell killing are well known through the damage that ionizing radiation does to DNA. The amount of radiation can be tracked through measuring the dose of the clinical photon or electron beam used. However, there are limitations in the usage of radiation therapy, for example, a tumor can create hypoxic areas that become radioresistant leading to complete ine↵ectiveness of further radiation treatment. This project looks into the application of cold atmospheric plasma as an adjuvant therapy …

Sense Of Belonging As Behaviorist Construct: “Doing Belonging” And Its Application For College Students With Disabilities, Chris Parthemos Phd

Theses and Dissertations

Sense of Belonging has been widely linked to academic performance, retention and persistence for college students. Students with disabilities are less likely to enroll than peers, and are more likely to drop out. Similar to other historically-marginalized groups, these students report lower sense of belonging than peers. Research with this population suggests that they may be seeking ways of building community and belonging at their universities which are self-driven. This notion of “doing belonging”, as it is described in the sociological literature, appears at first glance to be compatible with behaviorist approaches to student support. Although not without controversy, behavior …

Tgif1 Physiological Levels Limit Β-Cell Distress And Neonatal Diabetes, Creighton J. Friend

Theses and Dissertations

TGIF1 belongs to the superfamily of homeodomain proteins, which regulate a wide variety of biological functions, including cell stemness and specification of cell fate during early development. Perhaps surprisingly, we found that enforced expression in pancreatic progenitor cells during embryogenesis resulted in severe diabetes, hinting at the possibility that TGIF1 might regulate pancreas development. Subsequent genetic experiments targeting β-cells showed that TGIF1 affected β-cell function and homeostasis. Transcriptomic analysis revealed that TGIF1 expression inhibits the expression of essential components of UPR signaling, underscoring a potential mechanism in which TGIF1 disrupts protein folding and secretion. Congruently, TGIF1 expression led to a …

Buckets In A Deep Well: Essays About Shakespeare’S History Play Characters And An Original Drama, Brian C. Bassett

Theses and Dissertations

Buckets in a Deep Well is a set of essays that examines various characters portrayed in Shakespeare’s history plays, as well as parallel figures in 20th and 21st century works. Each essay primarily focuses on a single character’s response to a power structure: Richard Gloucester to the Tudor England of his audience in Richard III; Richard Nixon’s campaign-produced version of himself to 20th century American democracy in “Checkers;” Falstaff to organized society in Henry IV, Part 1; Marlo Stanfield to the drug economy of early 2000’s Baltimore in The Wire; the Duke of York to the disrupted monarchy of The …

Effectiveness Of Financial Incentives In Improving Breast Cancer Screening Among Medicaid Recipients, James B. Sherwood

Theses and Dissertations

Breast cancer remains one of the leading causes of death among women. Roughly 2.4 million women are diagnosed annually with breast cancer throughout the world. Although breast cancer survival rates are favorable for the United States compared to other nations (90% 5-year survival for non-metastatic disease), it comes at a high cost. The United States spends an estimated $30 billion annually on breast cancer treatment. In addition, disparities in breast cancer survival rates also exist in the United States. Women Medicaid recipients, who are predominately minorities (60%), are more likely to die of breast cancer due to their high rates …

Formation Of Vasculogenic Mimicry: Role Of Melanoma Differentiation Associated Gene-9 In Human Melanoma, Jinkal Shah

Theses and Dissertations

Malignant melanoma (MM) is the most aggressive skin cancer and the most frequent skin disorder in Caucasians. MM is associated with aggressive and progressive disease states, leading to major cancer-related morbidity and mortality. Recent investigations identify an alternative pathway -vasculogenic mimicry (VM), which is considered a cancer hallmark that can independently facilitate tumor neovascularization by the formation of fluid-conducting and vascular endothelial cells. MM cells undergoing VM can dedifferentiate into numerous cellular phenotypes and acquire endothelial-like features, resulting in the formation of the de novo matrix-rich vascular-like networks, such as plasma and red blood cells. The co-generation of endothelial cells, …

Wrestling With The One Drop Rule: Exploring Multiraciality In Social Work Education, Britney Pitts

Theses and Dissertations

This three paper dissertation aims to provide a baseline understanding of how multiraciality is understood in social work education. The first paper, a fusion autoethnography, explores how my own perception of my multiracial identity was clarified throughout my social work education. This exploration led to the development of incongruent logic in/validation (ILV), a new aspect of racial identity invalidation (RII). The second paper, a multimethod qualitative case study, explores master of social work (MSW) students’ knowledge, attitudes, and anticipated professional behaviors with multiracial individuals and families. Themes from this study include: never explicitly learned about multiraciality; non-ecological endorsements of multiracial …

Adapting Higher Education: Revamping Curricula For The Inclusion Of Theatre Students With Disabilities, Kevin Kemler

Theses and Dissertations

Equity, Diversity, and Inclusion initiatives in higher education have been largely driven by administrators who have little to no contact with the students for whom they are working for. This top-down approach negatively impacts marginalized students and disproportionately affects the quality of experience for students with Disabilities, an often-overlooked demographic. For Disabled students enrolled in performance programs, barriers to access and inclusion don’t just exist at the institutional level, they also exist in the traditional classroom or studio as well. Through a dismantling of ableist structures inherent within higher education (i.e., American grading practices, the Western and Theatrical Canons), I …

Children With Special Healthcare Needs: The Cost Of Medical Care, Kasey Dye

Theses and Dissertations

The purpose of this study was to explore factors that impact children with special healthcare needs’ (CSHCN) health insurance coverage and out-of-pocket medical expenses. The Ecological Model for Health Promotion developed by McLeroy et al. (1988) was the guiding theoretical framework for this study. Using data from the 2020 National Survey of Children’s Health, I conducted descriptive statistics, logistic regressions, and multilevel modeling analyses to determine if health insurance coverage and out-of-pocket expenses for CSHCN differed by individual, family, and community, organization, and policy factors. Results indicated that race, gender, disorder category, insurance type, and family’s federal poverty level were …

The Age And Primary Reason For The First Dental Visit In Children With Special Health Care Needs, Amanda Dickerson

Theses and Dissertations

Purpose: The aim of this project is to evaluate the average age and primary reason for the first dental visit among patients with special health care needs as well as to examine socioeconomic factors and other barriers related to children with special health care needs accessing care.

Methods: This is a cross-sectional survey that was distributed to caregivers of children with special health care needs. Study data was collected through REDCap, a secure web-based survey application. The inclusion criteria for participating in the survey included having a child with a special health care need that presented for a recall or …

Utilization Of Intraoral Scanners For Fabrication Of Obturators For Patients Born With Cleft Lip/ Palate (Clp), Swara Fadnis

Theses and Dissertations

Objective: The goal of this cross-sectional study was to assess the knowledge and attitude of providers regarding incorporating a digital scanner for impression taking in children born with cleft lip and/or palate (CLP). Methods: A survey was sent out to members of the American Association of Pediatric Dentistry (AAPD). The survey included questions regarding the providers’ perception of incorporating digital intraoral scanners for impressions for fabrication of obturators. The survey also included questions about the providers’ reservations in using this technology in practice, what their experience has been with the incorporation of this technology into practice, and what is …

Home Disruption And Substance Use: The Moderated-Mediating Role Of Family Conflict And Stress Among Racial-Ethnic Minoritized College Students, Geovani Muñoz

Theses and Dissertations

The COVID-19 pandemic has disproportionately impacted first-generation college students who identify as racial-ethnic minorities (REM). Aside from the academic and health repercussions caused by COVID-19, little is known if home disruptions have increased substance use for REM college students. Specifically, increased home disruptions may directly relate to substance use and indirect relationships through increased family conflict and stress. Therefore, this master's thesis examined the direct relationships between home disruption and substance use. I also examined the relationships between home disruption and family conflict, as well as home disruption and stress. Further, I also investigated the relationships between family conflict and …

The Wound Healing And Antibacterial Properties Of Mesenchymal Stromal Cell Extracellular Matrix Nanoparticles, Emily N. Wandling

Theses and Dissertations

Treatments for acute respiratory distress syndrome (ARDS) are still unavailable and the prevalence of the disease has only increased due to the Covid-19 pandemic. Mechanical ventilation regiments are still utilized to support declining lung function, but they also contribute to lung damage and increase the risk of bacterial infection. The anti-inflammatory and pro-regenerative abilities of mesenchymal stromal cells (MSCs) have shown to be a promising therapy for ARDS. We propose to utilize the regenerative effects of MSC secretome and the extracellular matrix (ECM) into a nanoparticle. Our mouse MSC (MMSC) ECM nanoparticles were characterized using size, zeta-potential, and mass spectrometry …

Combining Novel Direction Modulated Brachytherapy Tandem-And-Ovoids Applicators For Treatment Planning Of Multi-Institutional Cervical Cancer Cases: Removing Needles In Intracavitary-Interstitial Techniques, Sharmin Alam

Theses and Dissertations

Lack of standard guidelines for optimal needle insertion during high-dose-rate (HDR) intracavitary-interstitial (IC-IS) brachytherapy of the cervix means a sophisticated and technical skillset of inserting needles next to IC applicators must be developed to enhance plan quality. This study sought to evaluate the performance of two separate direction modulated brachytherapy (DMBT) tandem applicators used in conjunction with one set of novel DMBT ovoids, to effectively obviate the need for IS needles.

A cohort of 32 retrospective clinical HDR brachytherapy plans was re-planned with Varian’s BrachyVision^® treatment planning system, using VEGO^® inverse optimization algorithm, with dose heterogeneity accounted for …

An Introduction To Theatrical Scent Design As Compared To Traditional Design Areas With A Consideration For The Scientifically Notated Physical And Emotional Effects On The Human Body As It Potentially Relates To Future Creative Endeavors: A Framework, Katherine R. Holland

Theses and Dissertations

Traditional areas of theatrical design are established to the point of having a solid foundation for current and future designers to rely on as they strive to innovate. The same cannot be said for scent. I believe that art, like science, is a sector where the imagination is used to push the boundaries of what we currently know and can do. Artists are therefore entrusted with the responsibility to continue testing those boundaries, and that is what my topic lays the foundation for when it comes to scent. With my topic, I plan to look at the practical and ethical …

Predictive Modeling Of Thermophysical Properties Of Shocked Solid Diamond, Peter Muto

Theses and Dissertations

PREDICTIVE modeling of thermophysical properties of shocked solid diamond

Peter Muto and Gennady Miloshevsky

Virginia Commonwealth University, Department of Mechanical and Nuclear Engineering, 401 West Main St, Richmond, VA 23284-3015

Background

Shocks are high pressure waves that propagate in a material above the local speed of sound and induce severe pressure, density, and temperature changes, thus it is critical to be able to predict these effects.

Methods

The shock Hugoniot Equations of State (EoS) allow for the calculation of Hugoniot pressure and volume in a shocked material, but require a linear relationship between shock velocity and particle velocity, both experimentally …

Pre-Clinical Trials With Precision-Medicine Based Therapeutics In Basal-Like Patient-Derived Xenografts, David C. Boyd

Theses and Dissertations

Breast cancer treatments have improved over time, but the diseases seeing the most benefit from these improvements have the estrogen receptor, progesterone receptor, or are positive for HER2. Basal-like breast cancer tends to not have these biomarkers, which necessitates their treatment to be traditional, untargeted therapeutics which are less effective and tend to have harsh adverse effect profiles – this is an important unmet need. These studies utilize a variety of techniques, including tissue culture, viability assays, high-throughput screening, in vivo drug treatments and imaging, pathway analyses, molecular techniques such as Western blot, antibody arrays, RNA sequencing, sc RNA sequencing, …

Pms And Gas Pollutants Removal By Electret Filter Media And Metal-Organic Frameworks (Mofs), Yu Zhang

Theses and Dissertations

Indoor air quality (IAQ) has been becoming a major concern worldwide because statistical data showed that the indoor concentrations of gas pollutants, e.g., volatile organic compounds (VOCs) and particulate matters (PMs) are often 2–5 times higher than that of outdoors. Especially, people spend 87% of their time indoors and increasing to more than 90% during COVID-19 pandemic. The high concentration of these indoor air pollutants is mainly due to the reduced air exchange rate for the design of energy-efficient building construction and the increased usage of furnishings, glues, paints, furnaces, etc., leading to VOC outgassing and particle emission. Evidence has …

Characterization Of A Solid-State Detector For Dosimetry In The Diagnostic Energy Range With Verification Via Monte Carlo Estimation Of Average Breast Dose In Mammography, Areej Aljabal

Theses and Dissertations

This dissertation aims to develop a simplified QA metric for estimating radiation dose in mammography. This metric, the Mid-Breast Dose (MDB) Index, maybe be proposed as an alternative dose index, consistent with average glandular dose (AGD), for routine QA. MBD was obtained using “Phantom mid-point Air Kerma (AK) measurement” for conventional mammography. The advantage of this method is that MBD can be measured directly without requiring multiple conversion factors. The accuracy of clinical measurements in computing the MBD was assessed by comparing it with the AGD estimated by the Dance formalism experimentally and Monte Carlo simulation.

MBD methodology relies on …