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Mda-7/Il-24; A Promising Cancer Therapeutic Agent, Hossein Hamed Jun 2012

Mda-7/Il-24; A Promising Cancer Therapeutic Agent, Hossein Hamed

Theses and Dissertations

Glioblastoma multiforme (GBM) is an aggressive cancer that affects millions of patients per year. Conventional therapies combining chemotherapeutic agents with radiation can only extend survival by a few months; therefore, there is a dire need for an effective means of treating this deadly disease. Melanoma differentiation-associated gene-7/interleukin-24 (mda-7/IL-24), currently in the early stages of FDA pre-IND drug trials, has proven to be an effective cancer specific cytokine, able to trigger the onset of mitochondrial dysfunction and/or autophagy. GBM’s have mutations that often result in the activation of cytoprotective cell signaling pathways, preventing cancer therapeutics and even MDA-7/IL-24 treatments from being …

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Elucidating Genetic And Environmental Influences On Alcohol-Related Phenotypes, Jacquelyn Meyers Jun 2012

Elucidating Genetic And Environmental Influences On Alcohol-Related Phenotypes, Jacquelyn Meyers

Theses and Dissertations

Decades of work has led researchers to believe that risk for complex behavioral phenotypes, such as alcohol use disorders, is likely influenced by multiple genes of small effect acting in conjunction with each other and the environment. Currently, the field of psychiatric genetics is developing methodologies for the identification of genetic risk variants that predispose individuals to the development of complex behavioral disorders. Several challenges related to the complex and polygenic nature of these phenotypes, must be considered. This dissertation study attempts to address these important challenges in the context of alcohol use disorders and related phenotypes. A rich twin …

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Unique Features And Neuronal Properties In A Multisensory Cortex, W. Alex Foxworthy Jun 2012

Unique Features And Neuronal Properties In A Multisensory Cortex, W. Alex Foxworthy

Theses and Dissertations

UNIQUE FEAUTRES OF ORGANIZATION AND NEURONAL PROPERTIES IN A MULTISENSORY CORTEX Multisensory processing is a ubiquitous sensory effect that underlies a wide variety of behaviors, such as detection and orientation, as well as perceptual phenomena from speech comprehension to binding. Such multisensory perceptual effects are presumed to be based in cortex, especially within areas known to contain multisensory neurons. However, unlike their lower-level/primary sensory cortical counterparts, little is known about the connectional, functional and laminar organization of higher-level multisensory cortex. Therefore, to examine the fundamental features of neuronal processing and organization in the multisensory cortical area of the posterior parietal …

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The Effect Of Various Chemical Factors On Angiogenesis In The Chick Chorio-Allantoic Membrane, Heather Hammond Jun 2012

The Effect Of Various Chemical Factors On Angiogenesis In The Chick Chorio-Allantoic Membrane, Heather Hammond

Theses and Dissertations

The chick chorio-allantoic membrane (CAM) contains a complex vascular network commonly used to study angiogenesis. The application of chemical factors and oxygen barrier films onto this tissue can easily influence the process of angiogenesis. In this study, oxygen barrier film patches (Krehalon, polyvinylidene chloride, 12 μm thick, O2 transmission rate = 2.19 cm3•ml/100 in2•day•atm) were applied to areas of the CAM. Holes were made in the film and alginate beads incubated in various chemical factors were placed in the holes. After 24 and 48 hours of exposure to the alginate beads, images were taken of the tissue using a stereomicroscope …

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Otx But Not Mitf Transcription Factors Are Required For Zebrafish Rpe Development, Brandon Lane May 2012

Otx But Not Mitf Transcription Factors Are Required For Zebrafish Rpe Development, Brandon Lane

Theses and Dissertations

Mitf and Otx transcription factors have been identified as essential to the development of the retinal pigmented epithelium (RPE), but the relationship between these factors and their specific role in the RPE developmental pathway have not been clearly defined. The role of the two Mitf transcription factors (Mitfa and Mitfb) and two Otx transcription factors (Otx1a and Otx2) in zebrafish RPE development was explored in these experiments. The loss of Mitf activity in mitfa, mitfb, or double mitf null mutant fish lines had no effect on RPE pigmentation or development. The loss of Otx2 activity through morpholino knockdown produced a …

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An Agent Based Model Of Tumor Growth And Response To Radiotherapy, Nicole O'Neil May 2012

An Agent Based Model Of Tumor Growth And Response To Radiotherapy, Nicole O'Neil

Theses and Dissertations

An agent based model was developed to examine the growth of a tumor in a healthy cell population. Response to radiation and impact of mutation and bystander effects were studied. In the growth model, the cancer cells proliferated outward becoming invasive. The mass of cancer cells developed a necrotic core. Various treatment protocols of radiation were compared. Timing of treatments was critical to the success of therapy. The event of mutation was rare. When mutation occurred, either unsuccessful treatment or re-growth could result. Multiple rounds of radiation potentially led to increased mutation. Low levels of the bystander effect had little …

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The Antinociceptive Effects Of Alpha 7 Nicotinic Acetylcholine Receptor Positive Allosteric Modulators In Different Animal Pain Models, Kelen Freitas May 2012

The Antinociceptive Effects Of Alpha 7 Nicotinic Acetylcholine Receptor Positive Allosteric Modulators In Different Animal Pain Models, Kelen Freitas

Theses and Dissertations

The α7 nicotinic acetylcholine receptor (nAChR) subtype is abundantly expressed in the central nervous system (CNS) and in the periphery. Positive allosteric modulators (PAMs) of the α7 increase the response to an agonist and are divided into two types depending on whether they also decrease desensitization of the receptor (type II) or not (type I). Therefore, this study aims to investigate whether the enhancement of endogenous α7 nAChR function will result in a beneficial effect in nociceptive, inflammatory and chronic neuropathic pain models. While NS1738 and PNU-120596 were not active to reduce acute thermal pain, measured by hot-plate and tail-flick …

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T-Bet-Dependent Regulation Of T Cell Responses During Trypanosoma Cruzi Infection, Dustin Cobb May 2012

T-Bet-Dependent Regulation Of T Cell Responses During Trypanosoma Cruzi Infection, Dustin Cobb

Theses and Dissertations

The human pathogen Trypanosoma cruzi is an intracellular parasite and the etiological agent of Chagas disease. Protective immune responses to T. cruzi are highly dependent on T helper 1 and CD8+ T cells which produce interferon-gamma. A deficiency in these responses has severe consequences on the ability to control infection. Our investigation into the role of the Th1 transcription factor, T-bet, during murine T. cruzi infection revealed that T-bet is required for resistance. Contrary to our expectations, T-bet was not required for the development of Th1 immunity during infection, as T-bet-deficient mice still developed interferon-gamma-producing T cells. However, T-bet was …

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Validation Of 3'-Deoxy-3'-[18f]-Fluorothymidine Positron Emission Tomography For Image-Guidance In Biologically Adaptive Radiotherapy, Marian Axente May 2012

Validation Of 3'-Deoxy-3'-[18f]-Fluorothymidine Positron Emission Tomography For Image-Guidance In Biologically Adaptive Radiotherapy, Marian Axente

Theses and Dissertations

Accelerated tumor cell repopulation during radiation therapy is one of the leading causes for low survival rates of head-and-neck cancer patients. The therapeutic effectiveness of radiotherapy could be improved by selectively targeting proliferating tumor subvolumes with higher doses of radiation. Positron emission tomography (PET) imaging with 3´-deoxy-3´-[18F]-fluorothymidine (FLT) has shown great potential as a non-invasive approach to characterizing the proliferation status of tumors. This thesis focuses on histopathological validation of FLT PET imaging specifically for image-guidance applications in biologically adaptive radiotherapy. The lack of experimental data supporting the use of FLT PET imaging for radiotherapy guidance is addressed by developing …

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Reflections Of Self, Melissa Athey May 2012

Reflections Of Self, Melissa Athey

Theses and Dissertations

I vacillate between all extremes, beauty vs. ugly, internal vs. external, micro vs. macro. It is these disparate notions that inspire what I make. We cannot ever see ourselves objectively, but does that mean we shouldn’t try? This thesis is my attempt to dissect what I created in my 2 years at Virginia Commonwealth University, my exploration of the illness within and the psychological nature of how we go about hiding our insecurities.

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Racial Socialization In A Black-White Interracial Family In Virginia, Rebecca Hubbard May 2012

Racial Socialization In A Black-White Interracial Family In Virginia, Rebecca Hubbard

Theses and Dissertations

The Black-White biracial population is the largest and fastest growing subgroup of multiracial individuals in the United States. Despite substantial research literature on the positive relationship between Black racial identity and psychological wellbeing, Black-White biracial individuals are underrepresented in studies that provide evidence for this relationship. Root (1998) put forward an Ecological Metamodel of Biracial Identity, comprised of several factors related to contextual (e.g. class, regional history of race-relations, parental identity, extended family) and intra-psychic processes (e.g. social skills, coping skills). The ecological metamodel served as the theoretical framework for the current study. This study is a comprehensive investigation of …

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Symptom Clusters In Lung Cancer Patients, Debra Rattican May 2012

Symptom Clusters In Lung Cancer Patients, Debra Rattican

Theses and Dissertations

SYMPTOM CLUSTERS IN LUNG CANCER PATIENTS By Debra Rattican, PhD, RN A dissertation submitted in partial fulfillment of the requirements for the degree of Doctor of Philosophy at Virginia Commonwealth University. Virginia Commonwealth University, 2012 Major Director: Debra E. Lyon, PhD. Professor and Chair Family and Community Health Nursing The purpose of the study was to examine selected relationships among symptoms common to individuals with lung cancer. The specific aims were: 1) To examine the relationship between the symptoms of dyspnea and anxiety in patients with lung cancer. 2) To examine the relationships among the symptoms of dyspnea, anxiety, and …

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Optimization Of Feature Selection In A Brain-Computer Interface Switch Based On Event-Related Desynchronization And Synchronization Detected By Eeg, Mason Montgomery May 2012

Optimization Of Feature Selection In A Brain-Computer Interface Switch Based On Event-Related Desynchronization And Synchronization Detected By Eeg, Mason Montgomery

Theses and Dissertations

There are hundreds of thousands of people who could benefit from a Brain-Computer Interface. However, not all are willing to undergo surgery, so an EEG is the prime candidate for use as a BCI. The features of Event-Related Desynchronization and Synchronization could be used for a switch and have been in the past. A new method of feature selection was proposed to optimize classification of active motor movement vs a non-active idle state. The previous method had pre-selected which frequency and electrode to use as electrode C3 at the 20Hz bin. The new method used SPSS statistical software to determine …

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Synthesis And Biological Evaluation Of Second Generation Anibamine Analogues As Novel Anti-Prostate Cancer Agents, Shilpa Singh May 2012

Synthesis And Biological Evaluation Of Second Generation Anibamine Analogues As Novel Anti-Prostate Cancer Agents, Shilpa Singh

Theses and Dissertations

Prostate cancer is the most prevalent non-cutaneous cancer among men. Since the 19th century when Virchow first introduced the concept of inflammation in cancer, chemokines and their receptors have garnered a lot of interest. Chemokine receptor CCR5 has been especially implicated in many disease states and recently found to be over expressed in prostate cancer cell lines. Anibamine, a natural CCR5 antagonist discovered in 2004, has been found to have significant anti-prostate cancer activity at micromolar level. To optimize this compound and also discover a novel pharmacophore, exploration of the original structure was carried out. Significant modifications were made to …

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The Need For Collaboration In Planning Efforts During Natural Disasters: An Evaluation Of The City Of Richmond, Virginia, Jocelyn Leitch May 2012

The Need For Collaboration In Planning Efforts During Natural Disasters: An Evaluation Of The City Of Richmond, Virginia, Jocelyn Leitch

Theses and Dissertations

This thesis evaluates whether or not information data sharing is effectively used between federal, state, and local government agencies and non-governmental agencies in a metropolitan area during and immediately after a major natural disaster. Also, whether vulnerable populations were identified and considered during emergency management. The chosen study area is the City of Richmond, VA, and the disaster response is based on flooding episodes that occurred in the city over the last decade following hurricanes and tropical depressions. Questionnaires were administered to representatives of federal, state, and local agencies and NGOs. The questionnaires consisted of a Likert-style series of 10 …

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Reckless Hope, Jesse Potts May 2012

Reckless Hope, Jesse Potts

Theses and Dissertations

This thesis is an approximation of several years spent in the studio. It represents an equitable exercise of memory and speculation.

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The Dragons In Your Dreams, Jacquelyn Walther May 2012

The Dragons In Your Dreams, Jacquelyn Walther

Theses and Dissertations

What if every craving that you ever felt stayed with you forever? Suppose facades couldn’t exist, and what should only be the deepest, densest core of a desire was formed into a mass, a body. Its will matching yours in its physical presence. I make creatures that are embodiments of emotions. In this study I mainly focus on desires and inhibitions surrounding romance. I explore anatomical structures and how material make-up can directly reflect emotional character. In this exploration, the materiality of clay allows for a thorough manipulation of the composition of the creatures. In this I am also able …

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Identification Of Latent Subgroups Of Obese Adolescents Enrolled In A Healthy Weight Management Program, Cassie Brode May 2012

Identification Of Latent Subgroups Of Obese Adolescents Enrolled In A Healthy Weight Management Program, Cassie Brode

Theses and Dissertations

In obesity research, it is assumed that the population is homogeneous. While this approach has yielded important insights, testing this supposition might reveal information that could impact our understanding of the phenomena and its treatment. In this study, data from obese teenagers (N = 248, Mean BMI percentile = 99%; Mean age = 13.9, SD = 1.8) who were predominantly minority (n = 182), female (n = 169), and enrolled in a weight loss intervention were analyzed. Latent profile analysis (LPA) was used to segment patients into groups based on their scores on PedsQL 4.0 scales (physical-, emotional-, social-, and …

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Efficacy Of Reach Forgiveness For Foreign And Virginia Students, Yin Lin May 2012

Efficacy Of Reach Forgiveness For Foreign And Virginia Students, Yin Lin

Theses and Dissertations

People agree that forgiveness is a virtue in essentially all countries. However, different cultures have different ideas about how willing one should forgive and under what circumstances. Although the study occurred in the USA, I recruited both foreign-extraction and Virginia born-and-raised female college students (N=102) to participate a six-hour REACH forgiveness intervention, promoting their forgiveness through psychoeducational groups. In my thesis, I investigated whether students of foreign extraction and Virginia-born students would respond similarly to the intervention. I operationalized culture in two ways—by country and by individual self-reported self-construal. I measured forgiveness using two measures—decisional forgiveness and emotional forgiveness. I …

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Function Of Insulin-Like Growth Factor Binding Protein 7 (Igfbp7) In Hepatocellular Carcinoma, Dong Chen May 2012

Function Of Insulin-Like Growth Factor Binding Protein 7 (Igfbp7) In Hepatocellular Carcinoma, Dong Chen

Theses and Dissertations

Title of Dissertation: FUNCTION OF INSULIN-LIKE GROWTH FACTOR BINDING PROTEIN 7(IGFBP7) IN HEPATOCELLULAR CARCINOMA By Dong Chen. Purpose: Hepatocellular carcinoma (HCC) is a highly virulent malignancy with no effective treatment, thus requiring the development of innovative and effective targeted therapies. The oncogene Astrocyte Elevated Gene-1 (AEG-1) plays a seminal role in hepatocarcinogenesis and profoundly downregulates Insulin-like Growth Factor Binding Protein-7 (IGFBP7). The present study focuses on analyzing potential tumor suppressor functions of IGFBP7 in HCC and the relevance of IGFBP7 downregulation in mediating AEG-1 function. Experimental Design: IGFBP7 expression was detected by immunohistochemistry in HCC tissue microarrays by real-time PCR …

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Population Based Model Of Gonorrhea And Interventions Against Increased Antibiotic Resistance, Courtney Henry May 2012

Population Based Model Of Gonorrhea And Interventions Against Increased Antibiotic Resistance, Courtney Henry

Theses and Dissertations

Gonnorrhea is an infectious sexually transmitted disease (STD) caused by the bacterium Neisseria gonorrhoeae that commonly reproduces in the reproductive tract.The Centers of Disease Control and Prevention (CDC) estimate that more than 700,000 individuals in the U.S. contract new gonorrheal infections per year. During recent years, there has been a progressive global increase of drug-resistant strains of gonorrhea. Therefore, there exists the necessity for health organizations to encourage the monitoring, research and development of innovative treatment regimens. We have developed multiple mathematical models to explore the gonorrheal disease state. The first objective of model formulation was to fit the model …

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Characterization Of Superhydrophobic Surfaces Fabricated Using Ac-Electrospinning And Random Particle Deposition, Mohamed Samaha Jr. May 2012

Characterization Of Superhydrophobic Surfaces Fabricated Using Ac-Electrospinning And Random Particle Deposition, Mohamed Samaha Jr.

Theses and Dissertations

Surfaces with static contact angle greater than 150 degrees are typically classified as superhydrophobic. Such coatings have been inspired by the lotus leaf. As water flows over a superhydrophobic surface, "slip effect" is produced resulting in a reduction in the skin-friction drag exerted on the surface. Slip flow is caused by the entrapment of a layer of air between water and the surface. Superhydrophobicity could be utilized to design surfaces for applications such as energy conservation, noise reduction, laminar-to-turbulent-transition delay, and mixing enhancement. A popular method of manufacturing a superhydrophobic surface is microfabrication in which well-designed microgrooves and/or poles are …

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N-Terminal Processing Of Ribosomal Protein L27 In Staphylococcus Aureus, J. Harry Caufield May 2012

N-Terminal Processing Of Ribosomal Protein L27 In Staphylococcus Aureus, J. Harry Caufield

Theses and Dissertations

The bacterial ribosome is essential to cell growth yet little is known about how its proteins attain their mature structures. Recent studies indicate that certain Staphlyococcus aureus bacteriophage protein sequences contain specific sites that may be cleaved by a non-bacteriophage enzyme (Poliakov et al. 2008). The phage cleavage site was found to bear sequence similarity to the N-terminus of S. aureus ribosomal protein L27. Previous studies in E. coli (Wower et al.1998; Maguire et al. 2005) found that L27 is situated adjacent to the ribosomal peptidyl transferase site, where it likely aids in new peptide formation. The predicted S. aureus …

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Impacts Of Rdx Soil Contamination Across An Age Gradient For The Native Shrub Morella Cerifera., Stephen Via May 2012

Impacts Of Rdx Soil Contamination Across An Age Gradient For The Native Shrub Morella Cerifera., Stephen Via

Theses and Dissertations

Understanding the impacts of explosive contamination on vegetation is key to understanding explosives behavior in the environment. I quantified shrub growth responses to 1,3,5-trinitroperhydro-1,35-triazine (RDX) soil contamination across three life stages and I hypothesized that RDX would have the greatest impact on seed germination. Morella cerifera seeds were germinated on soils amended with RDX up to 1500 mg RDX kg-1 dry soil. Juvenile and adult individuals were exposed for 6 weeks to soil amended with RDX up to 750 and 1500 mg RDX kg-1 dry soil, respectively. Morphological responses were quantified for juveniles while physiological measurements were quantified for adults. …

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Design, Fabrication, And Testing Of A Pdms Micropump With Moving Membranes, Charles Cartin May 2012

Design, Fabrication, And Testing Of A Pdms Micropump With Moving Membranes, Charles Cartin

Theses and Dissertations

This paper will discuss the design, fabrication, and testing of a Poly(dimethylsiloxane) (PDMS) microfluidic pump. PDMS is commonly described as a soft polymer with very appealing chemical and physical properties such as optical transparency, low permeability to water, elasticity, low electrical conductivity, and flexible surface chemistry. PDMS microfluidic device fabrication is done easily with the use of soft lithography and rapid prototyping. PDMS microfluidic devices make it easier to integrate components and interface devices with particular users, than using typically harder materials such as glass and silicon. Fabrication and design of single and multilayer PDMS microfluidic devices is much easier …

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Comparison Of Root Canal Irrigation Systems In Reducing Intracanal Microorganisms Using Saline-An In Vitro Study, Pranav Desai May 2012

Comparison Of Root Canal Irrigation Systems In Reducing Intracanal Microorganisms Using Saline-An In Vitro Study, Pranav Desai

Theses and Dissertations

Mechanical and chemical debridement plays an important role in reducing intracanal microorganisms. Effective root canal irrigation depends on both the root canal irrigant and irrigation system. The objective of this study was to evaluate the debridement efficiency of four root canal irrigation systems, Endovac®, PiezoFlow™, EndoActivator® and traditional needle irrigation using saline as an irrigant. Seventy-five, single canal, extracted, mature teeth were selected. Teeth were standardized to canal lengths of 15 mm and instrumented to Master Apical File size #40 with 4% taper. Teeth were mounted in a centrifuge tube using PVS impression material. Teeth were randomly divided into four …

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Spatial Patterns Of Herbaceous And Woody Recruitment In A Recently Restored Mixed Tidal Regime Freshwater Wetland, James B. Deemy May 2012

Spatial Patterns Of Herbaceous And Woody Recruitment In A Recently Restored Mixed Tidal Regime Freshwater Wetland, James B. Deemy

Theses and Dissertations

Ecological restoration of a converted wetland was characterized within a recently drained impoundment along the James River in Charles City County, Virginia. Colonizing vegetation was assessed over three growing seasons in both tidal and non-tidal environments. Study objectives were to (1) examine geospatial relations of recruitment patterns among colonizing species over three growing seasons, (2) quantify species composition and potential differences between extant species cover and soil seed banks across restored and natural wetland habitats and (3) assess geospatial patterns to develop a GIS model of bald cypress (Taxodium distichum L.) recruitment. The two most common native colonizing species during …

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Mapping Women's Movement In Medieval England, Claire Clement May 2012

Mapping Women's Movement In Medieval England, Claire Clement

Theses and Dissertations

This thesis investigates women’s geographical movement in medieval England from the perspective of mobility and freedom. It uses pilgrimage accounts from medieval miracle story collections and to gather information about individual travel patterns. The study uses GIS to analyze gendered mobility patterns, and to investigate whether there were noticeable differences in the distance which men and women traveled and the geographical area of the country they originated. It also analyzes the nearness of men’s and women’s respective origin towns to alternative pilgrimage locations, as a means of examining the factors determining gendered travel mobility. The study finds that women’s travel …

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Expression Of Foreign Genes In The Pseudomonas Bacteriophage Pf3, Krystin Weathers May 2012

Expression Of Foreign Genes In The Pseudomonas Bacteriophage Pf3, Krystin Weathers

Theses and Dissertations

Filamentous bacteriophages were engineered to express foreign genes with the ultimate purpose of displaying transmission control anti-malarial peptides as in phage display. It was hypothesized that expression of foreign genes would be possible using the phage’s promoters. This hypothesis was tested by assuming that promoters for the phage major coat protein (MCP) gene would also promote the expression of any foreign gene inserted downstream of the MCP gene. As proof of principle, the bacteriophages Pf3, Pf1, and M13 were engineered in this way to successfully synthesize Enhanced Green Fluorescent Protein (EGFP). Type 88 phage display on the EGFP recombinant Pf3 …

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Radiation Sensitization Of Breast Cancer Cells By Vitamin D Through The Promotion Of Autophagic Cell Death, Eden Wilson May 2012

Radiation Sensitization Of Breast Cancer Cells By Vitamin D Through The Promotion Of Autophagic Cell Death, Eden Wilson

Theses and Dissertations

Radiation therapy is a widely used tool in cancer therapy and is frequently offered as the first line of treatment for cancers of the breast. While radiotherapy is often initially effective in killing tumor cells or suppressing their growth, there are factors that confer tumor cell resistance to irradiation. Development of resistance may lead to disease recurrence despite the use of surgery, chemotherapy and radiation therapy. A primary goal of the studies in Dr. Gewirtz’s laboratory is to develop strategies to overcome resistance to radiation (and chemotherapy) in breast cancer, with the ultimate goal of preventing or attenuating disease recurrence. …

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