Digital Commons Network^™

Topology And Dynamics Of Gene Regulatory Networks: A Meta-Analysis, Claus Kadelka

Biology and Medicine Through Mathematics Conference

No abstract provided.

Β Cell Network Dysfunction In Pancreatic Islets By Silencing Hub Cells, Janita Patwardhan, Bradford E. Peercy

Biology and Medicine Through Mathematics Conference

No abstract provided.

Coupled Influence Of Heart Rate Variability And Subcellular Calcium Heterogeneity On Cardiac Electromechanical Dynamics, Vrishti M. Phadumdeo, Seth H. Weinberg Ph.D

Biology and Medicine Through Mathematics Conference

No abstract provided.

Immunofluorescence Image Feature Analysis And Clustering Pipeline For Distinguishing Epithelial-Mesenchymal Transition, Shreyas Hirway, Nadiah Hassan, Dr. Christopher Lemmon, Dr. Seth Weinberg

Biology and Medicine Through Mathematics Conference

No abstract provided.

Non-Mechanistic Learning Of Pdes From Discrete Spatial Data In Biology, Gary M. Lavigne Jr

Biology and Medicine Through Mathematics Conference

No abstract provided.

Real-Time Interactive Simulation Of Human Atrial Electrical Activity On Realistic 3d Atrial Structures Using Webgl, Abouzar Kaboudian, Yanyan Ji, Elizabeth Cherry, Flavio H. Fenton

Biology and Medicine Through Mathematics Conference

No abstract provided.

[2019-03-22] Meeting Of The Academic And Health Affairs Policy Committee, Virginia Commonwealth University. Board Of Visitors

VCU Board of Visitors Minutes

Official minutes of the VCU Board of Visitors and its Executive Committee.

[2019-03-22] Meeting Of The Board Of Visitors Of Virginia Commonwealth University, Virginia Commonwealth University. Board Of Visitors

VCU Board of Visitors Minutes

Official minutes of the VCU Board of Visitors and its Executive Committee.

Commonwealth Times 2019-02-27

Commonwealth Times, 1969-

No abstract provided.

Commonwealth Times 2019-02-13

Commonwealth Times, 1969-

No abstract provided.

Commonwealth Times 2019-01-30

Commonwealth Times, 1969-

No abstract provided.

Virginia Commonwealth University Undergraduate Bulletin [2019], Virginia Commonwealth University

VCU Bulletins

Undergraduate bulletin for Virginia Commonwealth University for the academic year 2019-2020. It includes information on academic regulations, degree requirements, course offerings, faculty, academic calendar, and tuition and expenses for undergraduate programs.

Virginia Commonwealth University Courses [2019], Virginia Commonwealth University

VCU Bulletins

Listing of courses for the 2019-2020 year.

Targeting Intracellular Calcium Stores Alleviates Neurological Morbidities In A Dfp-Based Rat Model Of Gulf War Illness, Laxmikant Deshpande, Edna Santos, Kristin Phillips, Robert Blair

Neurology Publications

Gulf War Illness (GWI) is a chronic multi-symptom disorder afflicting the veterans of the First Gulf War, and includes neurological symptoms characterized by depression and memory deficits. Chronic exposure to organophosphates (OP) is considered a leading cause for GWI, yet its pathobiology is not fully understood. We recently observed chronic elevations in neuronal Ca2+ levels ([Ca2+]i) in an OP- diisopropyl fluorophosphate (DFP) based rat model for GWI. This study was aimed at identifying mechanisms underlying elevated [Ca2+]i in this DFP model and investigating whether their therapeutic targeting could improve GWI-like neurological morbidities. Male Sprague-Dawley rats (9-wks) were exposed to DFP …

Evolutionary Relationships Of Courtship Songs In The Parasitic Wasp Genus, Cotesia (Hymenoptera: Braconidae), Justin P. Bredlau, Karen M. Kester

Biology Publications

Acoustic signals play an important role in premating isolation based on sexual selection within many taxa. Many male parasitic wasps produce characteristic courtship songs used by females in mate selection. In Cotesia (Hymenoptera: Braconidae: Microgastrinae), courtship songs are generated by wing fanning with repetitive pulses in stereotypical patterns. Our objectives were to sample the diversity of courtship songs within Cotesia and to identify underlying patterns of differentiation. We compared songs among 12 of ca. 80 Cotesia species in North America, including ten species that have not been recorded previously. For Cotesia congregata, we compared songs of wasps originating from …

Optical Tweezers As An Effective Tool For Spermatozoa Isolation From Mixed Forensic Samples, Nicole Auka, Michael Valle, Bobby D. Cox, Peter D. Wilkerson, Tracey Dawson Cruz, Joseph E. Reiner, Sarah J. Seashols-Williams

Forensic Science Publications

A single focus optical tweezer is formed when a laser beam is launched through a high numerical aperture immersion objective. This objective focuses the beam down to a diffraction-limited spot, which creates an optical trap where cells suspended in aqueous solutions can be held fixed. Spermatozoa, an often probative cell type in forensic investigations, can be captured inside this optical trap and dragged one by one across millimeter-length distances in order to create a cluster of cells which can be subsequently drawn up into a capillary for collection. Sperm cells are then ejected onto a sterile cover slip, counted, and …

Electronic Nicotine Delivery Systems: Vaping Away Gum Tissue, Christina Tulloch, Denise Thieleman

Dental Hygiene Student Scholarship

Objective: Conventional cigarettes have shown severe toxicity on immune cells and wound healing in the periodontium, but little is known about the comparative effects of vaping or electronic cigarettes. In a substantial shift away from conventional cigarettes, vaping and e-cigarette sales have increased nearly 600% since 2017. If current conventional cigarette users are to transition to a less detrimental alternative, the evidence must demonstrate if electronic nicotine delivery systems can be deemed safer than conventional options.

Methods: By compiling data from the PubMed database, the most recent perspectives on new smoking methods and effects of usage on periodontal tissues were …

Shear Force Fiber Spinning: Process Parameter And Polymer Solution Property Considerations, Arzan C. Dotivala, Kavya P. Puthuveetil, Christina Tang

Chemical and Life Science Engineering Publications

For application of polymer nanofibers (e.g., sensors, and scaffolds to study cell behavior) it is important to control the spatial orientation of the fibers. We compare the ability to align and pattern fibers using shear force fiber spinning, i.e. contacting a drop of polymer solution with a rotating collector to mechanically draw a fiber, with electrospinning onto a rotating drum. Using polystyrene as a model system, we observe that the fiber spacing using shear force fiber spinning was more uniform than electrospinning with the rotating drum with relative standard deviations of 18% and 39%, respectively. Importantly, the approaches are complementary …

Rapid, Single-Step Protein Encapsulation Via Flash Nanoprecipitation, Shani L. Levit, Rebecca C. Walker, Christina Tang

Chemical and Life Science Engineering Publications

Flash NanoPrecipitation (FNP) is a rapid method for encapsulating hydrophobic materials in polymer nanoparticles with high loading capacity. Encapsulating biologics such as proteins remains a challenge due to their low hydrophobicity (logP < 6) and current methods require multiple processing steps. In this work, we report rapid, single-step protein encapsulation via FNP using bovine serum albumin (BSA) as a model protein. Nanoparticle formation involves complexation and precipitation of protein with tannic acid and stabilization with a cationic polyelectrolyte. Nanoparticle self-assembly is driven by hydrogen bonding and electrostatic interactions. Using this approach, high encapsulation efficiency (up to ~80%) of protein can be achieved. The resulting nanoparticles are stable at physiological pH and ionic strength. Overall, FNP is a rapid, efficient platform for encapsulating proteins for various applications.

Identifying Important Parameters In The Inflammatory Process With A Mathematical Model Of Immune Cell Influx And Macrophage Polarization, Marcella Torres, Jing Wang, Paul J. Yannie, Shobha Ghosh, Rebecca A. Segal, Angela M. Reynolds

Mathematics and Applied Mathematics Publications

In an inflammatory setting, macrophages can be polarized to an inflammatory M1 phenotype or to an anti-inflammatory M2 phenotype, as well as existing on a spectrum between these two extremes. Dysfunction of this phenotypic switch can result in a population imbalance that leads to chronic wounds or disease due to unresolved inflammation. Therapeutic interventions that target macrophages have therefore been proposed and implemented in diseases that feature chronic inflammation such as diabetes mellitus and atherosclerosis. We have developed a model for the sequential influx of immune cells in the peritoneal cavity in response to a bacterial stimulus that includes macrophage …

Glycosaminoglycans And Glycosaminoglycan Mimetics In Cancer And Inflammation, Shravan Morla

Medicinal Chemistry Publications

Glycosaminoglycans (GAGs) are a class of biomolecules expressed virtually on all mammalian cells and usually covalently attached to proteins, forming proteoglycans. They are present not only on the cell surface, but also in the intracellular milieu and extracellular matrix. GAGs interact with multiple ligands, both soluble and insoluble, and modulate an important role in various physiological and pathological processes including cancer, bacterial and viral infections, inflammation, Alzheimer’s disease, and many more. Considering their involvement in multiple diseases, their use in the development of drugs has been of significant interest in both academia and industry. Many GAG-based drugs are being developed …

Adapted K-Nearest Neighbors For Detecting Anomalies On Spatio–Temporal Traffic Flow, Youcef Djenouri, Asma Belhadi, Jerry Chun-Wei Lin, Alberto Cano

Computer Science Publications

Outlier detection is an extensive research area, which has been intensively studied in several domains such as biological sciences, medical diagnosis, surveillance, and traffic anomaly detection. This paper explores advances in the outlier detection area by finding anomalies in spatio-temporal urban traffic flow. It proposes a new approach by considering the distribution of the flows in a given time interval. The flow distribution probability (FDP) databases are first constructed from the traffic flows by considering both spatial and temporal information. The outlier detection mechanism is then applied to the coming flow distribution probabilities, the inliers are stored to enrich the …

Immersion In Physiology Bolsters Bedside Mechanistic Thinking, Rebecca Miller

Health Sciences Education Symposium

Purpose and Background:

• Compression of education and training with other responsibilities into more limited work hours
• Shift towards algorithm-based teaching/practice
• Less time spent in practice of basic science
• Intellectual Curiosity/Mechanistic Thinking less emphasized
• Self-directed learning and intellectual curiosity a primary focus for improvement in 2017

Mission Statement:

Through science experimentation in applied physiology, housestaff will reconnect with basic physiology and apply learned principles to commonly encouraged clinical scenarios.

Housestaff will participate in experiments to stimulate intellectual curiosity among participants and encourage appreciate and incorporation of physiology into their clinical and teaching roles.

Puberty: Is Your Gingiva Having Mood Swings?, Gabrielle R. Salvatore, Kendall A. Connerley

Dental Hygiene Student Scholarship

Objectives/aim: The purpose of this paper is to explore the effects on the different pathological changes in the oral cavity due to puberty, in both males and females. Hormonal changes caused by menstrual cycles, ovulation, the use contraceptives, and increased testosterone and estrogen levels.

Methods: This topic will be analyzed by thoroughly reviewing research on articles that relate to the oral health of individuals specifically between the ages of 12-18 years old.

Results: Research presents significant evidence that supports changes occurring in the oral cavity during an individual’s stage of puberty. These stages include ovulation, pre-menstruation, menstruation and males transitioning …

Dna Methylation Associated With Postpartum Depressive Symptoms Overlaps Findings From A Genome-Wide Association Meta-Analysis Of Depression, Dana M. Lapato, Roxann Roberson-Nay, Robert M. Kirkpatrick, Bradley T. Webb, Timothy P. York, Patricia A. Kinser

Human and Molecular Genetics Publications

Background Perinatal depressive symptoms have been linked to adverse maternal and infant health outcomes. The etiology associated with perinatal depressive psychopathology is poorly understood, but accumulating evidence suggests that understanding inter-individual differences in DNA methylation (DNAm) patterning may provide insight regarding the genomic regions salient to the risk liability of perinatal depressive psychopathology.

Results Genome-wide DNAm was measured in maternal peripheral blood using the Infinium MethylationEPIC microarray. Ninety-two participants (46% African-American) had DNAm samples that passed all quality control metrics, and all participants were within 7 months of delivery. Linear models were constructed to identify differentially methylated sites and regions, …

Virginia Commonwealth University Fall Commencement Program [2019], Virginia Commonwealth University

VCU Commencement Programs

No abstract provided.

Vcu Brew, The Grace E. Harris Leadership Institute At Virginia Commonwealth University, Denise Bullock, Lauren Dunn, Brian Fowlkes, Hermine Maes, Elizabeth Marlowe, Fernando Tenjo-Fernandez, Zakia Williams

The Grace E. Harris Leadership Institute Publications

VCU Brew is an innovative proposal to create a VCU branded signature beer. The Campus Connectors will present a rationale showing how this proposal will i) potentially generate revenue to benefit VCU scholarships and academic programs, ii) create opportunities for experiential learning by engaging VCU students in product development, branding, labeling, marketing, business, and awareness of responsible drinking, iii) and guide the process of partnering with local breweries to provide practical expertise and stimulate entrepreneurship initiatives that lead to distribution at VCU athletic, community and Alumni events, while increasing ties with the community and national prominence.

Metabolic Modulation Predicts Heart Failure Tests Performance, Daniel Contaifer Jr., Leo F. Buckley, George Wohlford, Naren G. Kumar, Joshua M. Morriss, Asanga D. Ranasinghe, Salvatore Carbone, Justin M. Canada, Cory Trankle, Antonio Abbate, Benjamin W. Van Tassell, Dayanjan S. Wijesinghe

Pharmacotherapy and Outcomes Science Publications

The metabolic changes that accompany changes in Cardiopulmonary testing (CPET) and heart failure biomarkers (HFbio) are not well known. We undertook metabolomic and lipidomic phenotyping of a cohort of heart failure (HF) patients and utilized Multiple Regression Analysis (MRA) to identify associations to CPET and HFBio test performance (peak oxygen consumption (Peak VO₂), oxygen uptake efficiency slope (OUES), exercise duration, and minute ventilation-carbon dioxide production slope (VE/VCO₂ slope), as well as the established HF biomarkers of inflammation C-reactive protein (CRP), beta-galactoside-binding protein (galectin-3), and N-terminal prohormone of brain natriuretic peptide (NT-proBNP)). A cohort of 49 patients with …

Homologous Recombination Under The Single-Molecule Fluorescence Microscope, Dalton R. Gibbs, Soma Dhakal

Chemistry Publications

Homologous recombination (HR) is a complex biological process and is central to meiosis and for repair of DNA double-strand breaks. Although the HR process has been the subject of intensive study for more than three decades, the complex protein–protein and protein–DNA interactions during HR present a significant challenge for determining the molecular mechanism(s) of the process. This knowledge gap is largely because of the dynamic interactions between HR proteins and DNA which is difficult to capture by routine biochemical or structural biology methods. In recent years, single-molecule fluorescence microscopy has been a popular method in the field of HR to …

Evaluating Neighborhood, Social, And Genetic Influences On Precursors Of Alcohol Use Risk Behavior In African American Adolescents, Kimberly S. Compton, Peter Barr, Katherine J. Karriker-Jaffe, Cristina Bares, Fazil Aliev, Brian Mustanski, Danielle Dick, Karen G. Chartier

Social Work Publications

Background: Using a socioecological framework, we examined neighborhood and social stressors in concert with genetic risk for alcohol dependence in relation to externalizing behaviors, important precursors to alcohol-related problems.

Methods: We used data from African American adolescents and their caregivers in the Gene, Environment, and Neighborhood Initiative, a subsample of the Mobile Youth and Poverty Study. Participants for the current analyses included 112 adolescents who reported ever having at least one full drink of alcohol. Empirical Bayes scores were used to estimate neighborhood-level violence and transitions. Multivariate models tested main effects and then interactions of family stressors, discrimination, …