Digital Commons Network^™

On Applications And Limitations Of One-Dimensional Capillarity Formulations For Media With Heterogeneous Wettability, T. M. Bucher, H. Vahedi Tafreshi

Mechanical and Nuclear Engineering Publications

Force-balance-based one-dimensional algebraic formulations that are often used in characterizing the capillarity of a multi-component system (e.g., predicting capillary height rise inporous media) are discussed. It is shown that such formulations fail to provide accurate predictions when the distribution of wetting (or non-wetting) surfaces is not homogeneous. A more general mathematical formulation is suggested and used to demonstrate that for media with heterogeneous wettability, hydrophilic (or hydrophobic) surfaces clustered in groups will have less contribution to the overall capillarity of the system.

Increased Frequency Of Micronuclei In Adults With A History Of Childhood Sexual Abuse: A Discordant Monozygotic Twin Study, Timothy P. York, Jenni Brumelle, Jane Juusola, Kenneth S. Kendler, Lindon J. Eaves, Ananda B. Amstadler, Steven H. Aggen, Kimberly H. Jones, Andrea Ferreira-Gonzalez, Colleen Jackson-Cook

Human and Molecular Genetics Publications

Background

Childhood sexual abuse (CSA) is a traumatic life event associated with an increased lifetime risk for psychopathology/morbidity. The long-term biological consequences of CSA-elicited stress on chromosomal stability in adults are unknown. The primary aim of this study was to determine if the rate of acquired chromosomal changes, measured using the cytokinesis-block micronucleus assay on stimulated peripheral blood lymphocytes, differs in adult female monozygotic twins discordant for CSA.

Methods

Monozygotic twin pairs discordant for CSA were identified from a larger population-based sample of female adult twins for whom the experience of CSA was assessed by self-report (51 individuals including a …

Identification Of Genes Potentially Regulated By Human Polynucleotide Phosphorylase (Hpnpaseold-35) Using Melanoma As A Model, Upneet K. Sokhi, Manny D. Bacolod, Santanu Dasgupta, Luni Emdad, Swadesh K. Das

Human and Molecular Genetics Publications

Human Polynucleotide Phosphorylase (hPNPaseold-35 or PNPT1) is an evolutionarily conserved 3′→5′ exoribonuclease implicated in the regulation of numerous physiological processes including maintenance of mitochondrial homeostasis, mtRNA import and aging-associated inflammation. From an RNase perspective, little is known about the RNA or miRNA species it targets for degradation or whose expression it regulates; except for c-myc and miR-221. To further elucidate the functional implications of hPNPaseold-35 in cellular physiology, we knocked-down and overexpressed hPNPaseold-35 in human melanoma cells and performed gene expression analyses to identify differentially expressed transcripts. Ingenuity Pathway Analysis indicated that knockdown of hPNPaseold-35 resulted in significant …

The Krüppel-Like Factor 2 And Krüppel-Like Factor 4 Genes Interact To Maintain Endothelial Integrity In Mouse Embryonic Vasculogenesis, Aditi R. Chiplunkar, Benjamin C. Curtis, Gabriel L. Eades, Megan S. Kane, Sean J. Fox, Jack L. Haar, Joyce A. Lloyd

Human and Molecular Genetics Publications

Background Krüppel-like Factor 2 (KLF2) plays an important role in vessel maturation during embryonic development. In adult mice, KLF2 regulates expression of the tight junction protein occludin, which may allow KLF2 to maintain vascular integrity. Adult tamoxifen-inducible Krüppel-like Factor 4 (KLF4) knockout mice have thickened arterial intima following vascular injury. The role of KLF4, and the possible overlapping functions of KLF2 and KLF4, in the developing vasculature are not well-studied.

Results Endothelial breaks are observed in a major vessel, the primary head vein (PHV), in KLF2-/-KLF4-/- embryos at E9.5. KLF2-/-KLF4-/- embryos die by E10.5, which is earlier than either single …

Krüppel-Like Factor 2 Is Required For Normal Mouse Cardiac Development, Aditi R. Chiplunkar, Tina K. Lung, Yousef Alhashem, Benjamin A. Koppenhaver, Fadi N. Salloum, Rakesh C. Kukreja, Jack L. Haar, Joyce A. Lloyd

Human and Molecular Genetics Publications

Krüppel-like factor 2 (KLF2) is expressed in endothelial cells in the developing heart, particularly in areas of high shear stress, such as the atrioventricular (AV) canal. KLF2 ablation leads to myocardial thinning, high output cardiac failure and death by mouse embryonic day 14.5 (E14.5) in a mixed genetic background. This work identifies an earlier and more fundamental role for KLF2 in mouse cardiac development in FVB/N mice. FVB/N KLF2−/− embryos die earlier, by E11.5. E9.5 FVB/N KLF2−/− hearts have multiple, disorganized cell layers lining the AV cushions, the primordia of the AV valves, rather than the normal single layer. By …

Targeted Apoptotic Effects Of Thymoquinone And Tamoxifen On Xiap Mediated Akt Regulation In Breast Cancer, Shashi Rajput, B. N. P. Kumar, Siddik Sarkar, Subhasis Das, Belal Azab, Prasanna K. Santhekadur, Swadesh K. Das, Luni Emdad, Devanand Sarkar, Paul B. Fisher, Mahitosh Mandal

Human and Molecular Genetics Publications

X-linked inhibitor of apoptosis protein (XIAP) is constitutively expressed endogenous inhibitor of apoptosis, exhibit its antiapoptotic effect by inactivating key caspases such as caspase-3, caspase-7 and caspase-9 and also play pivotal role in rendering cancer chemoresistance. Our studies showed the coadministration of TQ and TAM resulting in a substantial increase in breast cancer cell apoptosis and marked inhibition of cell growth both in vitro and in vivo. Anti-angiogenic and anti-invasive potential of TQ and TAM was assessed through in vitro studies. This novel combinatorial regimen leads to regulation of multiple cell signaling targets including inactivation of Akt and XIAP degradation. …

Amelogenin Peptide Extract Increases Differentiation And Angiogenic And Local Factor Production And Inhibits Apoptosis In Human Osteoblasts, Rene Olivares-Navarrete, Sharon L. Hyzy, Argelia Almaguer-Flores, Corinna Mauth, Anja C. Gemperli, Barbara D. Boyan, Zvi Schwartz

Biomedical Engineering Publications

Enamel matrix derivative (EMD), a decellularized porcine extracellular matrix (ECM), is used clinically in periodontal tissue regeneration. Amelogenin, EMD’s principal component, spontaneously assembles into nanospheres in vivo, forming an ECM complex that releases proteolytically cleaved peptides. However, the role of amelogenin or amelogenin peptides in mediating osteoblast response to EMD is not clear. Human MG63 osteoblast-like cells or normal human osteoblasts were treated with recombinant human amelogenin or a 5 kDa tyrosine-rich amelogenin peptide (TRAP) isolated from EMD and the effect on osteogenesis, local factor production, and apoptosis assessed. Treated MG63 cells increased alkaline phosphatase specific activity and levels of …

Hiv Protease Inhibitors Disrupt Lipid Metabolism By Activating Endoplasmic Reticulum Stress And Inhibiting Autophagy Activity In Adipocytes, Beth S. Zha, Xiaoshan Wan, Xiaoxuan Zhang, Weibin Zha, Jun Zhou, Martin Wabitsch, Guangji Wang, Vijay Lyall, Phillip B. Hylemon, Huiping Zhou

Microbiology and Immunology Publications

Background

HIV protease inhibitors (PI) are core components of Highly Active Antiretroviral Therapy (HAART), the most effective treatment for HIV infection currently available. However, HIV PIs have now been linked to lipodystrophy and dyslipidemia, which are major risk factors for cardiovascular disease and metabolic syndrome. Our previous studies have shown that HIV PIs activate endoplasmic reticulum (ER) stress and disrupt lipid metabolism in hepatocytes and macrophages. Yet, little is known on how HIV PIs disrupt lipid metabolism in adipocytes, a major cell type involved in the pathogenesis of metabolic syndrome.

Methodology and Principal Findings

Cultured and primary mouse adipocytes and …

Virginia Commonwealth University Fall Commencement Program [Corrected Copy] [2013], Virginia Commonwealth University

VCU Commencement Programs

No abstract provided.

Virginia Commonwealth University Undergraduate Bulletin Courses [2013], Virginia Commonwealth University

VCU Bulletins

Listing of undergraduate courses for 2013-2014.

Seedfolks: An Integrated Literature And Visual Art Unit, Michi Thacker

Teaching Resource Archive

The book Seedfolks takes place in Cleveland, Ohio, in an inner-city vacant lot. The story develops through a series of 13 vignettes, each culturally isolated character bringing his/her unique perspective and contribution to the story as the trash-ridden lot slowly develops into a community garden.

Through And Beyond The Barbie: 3 Themed Lesson Sequences, Rabeya Jalil

Middle School Resources

This lesson sequence will facilitate students to collaborate, explore and experience themes that they can personally relate to on a deeper and meaningful level. The purpose of this unit is to foster a safe and creative environment for early adolescents of 11 – 14 years (7-8th grades) to explore their personal identities, their gender, their socio-cultural beliefs, their values, through critical thinking, self-reflecting, and art making. Through engaging in a conversation and exploratory dialogue to deconstruct the layers that form identity and culture, using both in the traditional and digital media (collage, printmaking, drawing, digital photography, video, Scratch programming software, …

The Influence Of Hypoxia And Ph On Bioluminescence Imaging Of Luciferase-Transfected Tumor Cells And Xenografts, Ashraf A. Khalil, Mark J. Jameson, William C. Broaddus, Peck Sun Lin, Seth M. Dever, Sarah E. Golding, Elisabeth Rosenberg, Kristoffer Valerie, Theodore D. Chung

Neurosurgery Publications

Bioluminescence imaging (BLI) is a relatively new noninvasive technology used for quantitative assessment of tumor growth and therapeutic effect in living animal models. BLI involves the generation of light by luciferase-expressing cells following administration of the substrate luciferin in the presence of oxygen and ATP. In the present study, the effects of hypoxia, hypoperfusion, and pH on BLI signal (BLS) intensity were evaluated in vitro using cultured cells and in vivo using a xenograft model in nude mice. The intensity of the BLS was significantly reduced in the presence of acute and chronic hypoxia. Changes in cell density, viability, and …

Virginia Commonwealth University Fall Commencement Program [2013], Virginia Commonwealth University

VCU Commencement Programs

No abstract provided.

Microglial Activation Decreases Retention Of The Protease Inhibitor Saquinavir: Implications For Hiv Treatment, Shannon Dallas, Michelle L. Block, Deborah M. Thompson, Marcelo G. Bonini, Patrick T. Ronaldson, Reina Bendayan, David S. Miller

Anatomy and Neurobiology Publications

Background

Active HIV infection within the central nervous system (CNS) is confined primarily to microglia. The glial cell compartment acts as a viral reservoir behind the blood-brain barrier. It provides an additional roadblock to effective pharmacological treatment via expression of multiple drug efflux transporters, including P-glycoprotein. HIV/AIDS patients frequently suffer bacterial and viral co-infections, leading to deregulation of glial cell function and release of pro-inflammatory mediators including cytokines, chemokines, and nitric oxide.

Methods

To better define the role of inflammation in decreased HIV drug accumulation into CNS targets, accumulation of the antiretroviral saquinavir was examined in purified cultures of rodent …

Virginia Commonwealth University Graduate And Professional Programs Bulletin Courses [2013], Virginia Commonwealth University

VCU Bulletins

Listing of professional and graduate courses for 2013-2014.

Cyanine 5.5 Conjugated Nanobubbles As A Tumor Selective Contrast Agent For Dual Ultrasound-Fluorescence Imaging In A Mouse Model, Liyi Mai, Anna Yao, Jing Li, Qiong Wei, Ming Yuchi, Xiaoling He, Mingyue Ding, Qibing Zhou

Medicinal Chemistry Publications

Nanobubbles and microbubbles are non-invasive ultrasound imaging contrast agents that may potentially enhance diagnosis of tumors. However, to date, both nanobubbles and microbubbles display poor in vivo tumor-selectivity over non-targeted organs such as liver. We report here cyanine 5.5 conjugated nanobubbles (cy5.5-nanobubbles) of a biocompatible chitosan–vitamin C lipid system as a dual ultrasound-fluorescence contrast agent that achieved tumor-selective imaging in a mouse tumor model. Cy5.5-nanobubble suspension contained single bubble spheres and clusters of bubble spheres with the size ranging between 400–800 nm. In the in vivo mouse study, enhancement of ultrasound signals at tumor site was found to persist over …

Must Do @ Vcu, Grace E. Harris Leadership Institute, Virginia Commonwealth University, Katherine Beck, Philip Branch, Tom Diehl, Joyce Lloyd, Claudia Mangum, Yaoying Xu

L. Douglas Wilder School of Government and Public Affairs Publications

Must Do @ VCU is a set of annual collegial activities that can be performed throughout the year, by faculty, staff and students. These VCU-centered activities are considered to be the things that give VCU its identity. The goal of Must Do @ VCU is to generate a sense of community and of belonging to the University. VCU is a relatively new University and its traditions are therefore not well-established. Must Do @ VCU aims to build on shared experiences as a method to establish VCU culture.

A Networks Method For Ranking Microrna Dysregulation In Cancer, William Budd, Sarah Seashols, Danielle Weaver, Cyriac Joseph, Zendra E. Zehner

Biochemistry and Molecular Biology Publications

Background

Despite the lack of agreement on their exact roles, it is known that miRNAs contribute to cancer progression. Many studies utilize methods to detect differential regulation of miRNA expression. It is prohibitively expensive to examine all potentially dysregulated miRNAs and traditionally, researchers have focused their efforts on the most extremely dysregulated miRNAs. These methods may overlook the contribution of less differentially expressed but more functionally relevant miRNAs. The purpose of this study was to outline a method that not only utilizes differential expression but ranks miRNAs based on the functional relevance of their targets. This work uses a networks …

Virginia Commonwealth University Commencement Program [2013], Virginia Commonwealth University

VCU Commencement Programs

No abstract provided.

Cultural Inheritance And Fertility Outcomes: An Analysis From Evolutionary And Interdisciplinary Perspectives, Li Zhang, Dudley L. Poston Jr., Michael S. Alvard, Christopher Cherry

L. Douglas Wilder School of Government and Public Affairs Publications

Taking evolutionary and interdisciplinary perspectives, this study views the reproductive result as an evolutionary outcome that may be affected by parental characteristics through cultural inheritance. We hypothesize that inheriting more cultural traits from parents leads to a greater resemblance between fertility outcomes of the offspring and their parents. In societies that experience a demographic transition, a greater resemblance can be indicated by a higher level of fertility of the offspring and a sooner transition from union formation to childbearing. We operationalize inheriting cultural traits from parents as reporting a religious affiliation the same as those of their parents. Through analyzing …

Isozyme-Specific Ligands For O-Acetylserine Sulfhydrylase, A Novel Antibiotic Target, Francesca Spyrakis, Ratna Singh, Pietro Cozzini, Enea Salsi, Paolo Felici, Samanta Raboni, Paolo Benedetti, Gabriele Cruciani, Glen E. Kellogg, Paul F. Cook, Andrea Mozzarelli

Medicinal Chemistry Publications

The last step of cysteine biosynthesis in bacteria and plants is catalyzed by O-acetylserine sulfhydrylase. In bacteria, two isozymes, O-acetylserine sulfhydrylase-A and O-acetylserine sulfhydrylase-B, have been identified that share similar binding sites, although the respective specific functions are still debated. O-acetylserine sulfhydrylase plays a key role in the adaptation of bacteria to the host environment, in the defense mechanisms to oxidative stress and in antibiotic resistance. Because mammals synthesize cysteine from methionine and lackO-acetylserine sulfhydrylase, the enzyme is a potential target for antimicrobials. With this aim, we first identified potential inhibitors of the two …

Contribution Of Nadph Oxidase To Membrane Cd38 Internalization And Activation In Coronary Arterial Myocytes, Ming Xu, Xiao-Xue Li, Joseph K. Ritter, Justine M. Abais, Yang Zhang, Pin-Lan Li

Pharmacology and Toxicology Publications

The CD38-ADP-ribosylcyclase-mediated Ca2+ signaling pathway importantly contributes to the vasomotor response in different arteries. Although there is evidence indicating that the activation of CD38-ADP-ribosylcyclase is associated with CD38 internalization, the molecular mechanism mediating CD38 internalization and consequent activation in response to a variety of physiological and pathological stimuli remains poorly understood. Recent studies have shown that CD38 may sense redox signals and is thereby activated to produce cellular response and that the NADPH oxidase isoform, NOX1, is a major resource to produce superoxide (O2·−) in coronary arterial myocytes (CAMs) in response to muscarinic receptor agonist, which uses CD38-ADP-ribosylcyclase …

Enhanced Biocidal Activity Of Au Nanoparticles Synthesized In One Pot Using 2, 4-Dihydroxybenzene Carbodithioic Acid As A Reducing And Stabilizing Agent, Syeda Sohaila Naz, Nazar Ul Islam, Muhammad Raza Shah, Syed Sartaj Alam, Zafar Iqbal, Massimo Bertino, Louis Franzel, Afifa Ahmed

Physics Publications

Background

The conjugation of gold nanoparticles with biocides such as natural products, oligosaccharides, DNA, proteins has attracted great attention of scientists recently. Gold NPs covered with biologically important molecules showed significant enhancement in biological activity in comparison with the activity of the free biocides. However, these reports are not very systematic and do not allow to draw definitive conclusions. We therefore embarked in a systematic study related to the synthesis and characterization of biocidal activities of Au nanoparticles conjugated to a wide variety of synthetic and natural biomolecules. In this specific report, we investigated the activity of a synthetic biocide, …

Quantification Of Biomass And Cell Motion In Human Pluripotent Stem Cell Colonies, Thomas A. Zangle, Jennifer Chun, Jin Zhang, Jason C. Reed, Michael A. Teitell

Physics Publications

Abstract

Somatic cell reprogramming to pluripotency requires an immediate increase in cell proliferation and reduction in cell size. It is unknown whether proliferation and biomass controls are similarly coordinated with early events during the differentiation of pluripotent stem cells (PSCs). This impasse exists because PSCs grow in tight clusters or colonies, precluding most quantifying approaches. Here, we investigate live cell interferometry as an approach to quantify the biomass and growth of HSF1 human PSC colonies before and during retinoic acid-induced differentiation. We also provide an approach for measuring the rate and coordination of intracolony mass redistribution in HSF1 clusters using …

Enhancing The Benefits Of Written Emotional Disclosure Through Response Training, Andrea Konig, Alison Eonta, Stephanie R. Dyal, Scott R. Vrana

Psychology Publications

Writing about a personal stressful event has been found to have psychological and physical health benefits, especially when physiological response increases during writing. Response training was developed to amplify appropriate physiological reactivity in imagery exposure. The present study examined whether response training enhances the benefits of written emotional disclosure. Participants were assigned to either a written emotional disclosure condition (n = 113) or a neutral writing condition (n = 133). Participants in each condition wrote for 20 minutes on three occasions and received response training (n = 79), stimulus training (n = 84) or no training (n = 83). Heart …

Biosynthesis Of Vitamins And Cofactors In Bacterium-Harbouring Trypanosomatids Depends On The Symbiotic Association As Revealed By Genomic Analyses, Cecilia C. Klein, Joao M. P. Alves, Myrna G. Serrano, Gregory A. Buck, Ana T. R. Vasconcelos, Marie-France Sagot, Marta M. G. Teixeira, Emey P. Camargo, Maria C. M. Motta

Study of Biological Complexity Publications

Some non-pathogenic trypanosomatids maintain a mutualistic relationship with a betaproteobacterium of the Alcaligenaceae family. Intensive nutritional exchanges have been reported between the two partners, indicating that these protozoa are excellent biological models to study metabolic co-evolution. We previously sequenced and herein investigate the entire genomes of five trypanosomatids which harbor a symbiotic bacterium (SHTs for Symbiont-Haboring Trypanosomatids) and the respective bacteria (TPEs for Trypanosomatid Proteobacterial Endosymbiont), as well as two trypanosomatids without symbionts (RTs for Regular Trypanosomatids), for the presence of genes of the classical pathways for vitamin biosynthesis. Our data show that genes …

Pilot Inventory Of Community Partnerships, Valerie Holton

Division of Community Engagement Resources

Partnerships with community organizations create increased opportunities for universities to promote community-engaged research, teaching, and service. Virginia Commonwealth University’s (VCU) mission states that the university is committed to "sustainable, university-community partnerships that enhance the educational, economic, and cultural vitality of the communities VCU serves." In recognition of its efforts, VCU is among a small number of institutions to be designated as very high research activity and community-engaged by the Carnegie Foundation. It is also a recipient of a NIH-funded Center for Translational and Sciences Award, which promotes community-engaged research.

In pursuit of the university’s goal to become a national model …

An Examination Of Project Based Learning At The Secondary Level: A Review Of Literature, Candace Mirabile

MERC Publications

Project based learning (PBL) is a pedagogical approach designed to capture student interest by integrating a contemporary and relevant problem or issue with content standards. The expectation is that students who have not been successful under traditional teacher‐ centered/ lecture‐oriented instruction will be taken in by the opportunity to investigate a topic of personal interest using PBL. Moreover, some say that when constructivist learning (upon which PBL is based) is designed to reflect contemporary research methods and design protocols, students will be better prepared for the 21st century workplace (Apedoe, Reynolds, Ellefson & Schunn, 2008; Bell, 2010; Technology Assistance Program, …

2013 Service-Learning Faculty Survey Results Report, Office Of Service-Learning, Division Of Community Engagement, Virginia Commonwealth University

Division of Community Engagement Resources

Service-learning is an important teaching pedagogy that has been proven effective in enhancing student learning and promoting both academic and civic engagement. As civic engagement continues to gain prominence as an integral aspect of higher education, it is increasingly important that engagement metrics are consistently measured to demonstrate outcomes. This is especially true of service-learning as it strongly correlates with student learning, academic performance, and retention rates. Feedback and suggestions from service-learning faculty members are useful for gaining insights into how service-learning quality can be enhanced at the classroom level and understanding the resource needs of faculty members.

The purpose …