Digital Commons Network^™

Offering The Choice Of Self-Administered Oral Hiv Testing (Chivst) Among Long-Distance Truck Drivers In Kenya: A Trial-Based Cost-Effectiveness Analysis, Deo Mujwara, April D. Kimmel

Graduate Research Posters

Background: Long distance truck drivers (LDTD) are a high-HIV-risk population facing unique healthcare barriers due to continuous travel and irregular schedules, and may require targeted, resource-intensive strategies for HIV-test uptake. We conducted a trial-based cost-effectiveness of CHIVST among LDTD in Kenya.

Methods: Effectiveness data came from a randomized-controlled trial of CHIVST (n=150) versus provider-administered testing (n=155). Economic cost data came from the literature and reflected a societal perspective. Generalized Poisson and linear-gamma regression models estimated the effectiveness (relative-risk) and incremental costs (2017 I$), respectively, with incremental effectiveness calculated as the reciprocal of the absolute risk difference and reported as the …

Supporting Student Mental Health During And After Covid-19, David Bryant Naff, Shenita Williams, Jenna Furman, Melissa Lee

MERC Publications

This report by the Metropolitan Educational Research Consortium (MERC) in the VCU School of Education offers a rapid review of research about supporting student mental health as they return to school during COVID-19. It pulls from literature on natural disasters like hurricane Katrina, the psychological impacts of quarantine, and emergent research on the mental health impacts of the Coronavirus. The report is structured to answer three overarching questions: 1) Why is it important to address the mental health needs of students in schools? 2) How can we expect COVID-19 to impact the mental health of students? 3) What are some …

Characterizing A20 Function In Periodontal Tissue Homeostasis, Erin Mooney

Theses and Dissertations

The regulation of inflammatory signaling pathways is critical for proper innate immune responses to pathogens as well as the prevention of chronic inflammation. Ubiquitination, a covalent posttranslational modification, has been identified as a key regulator of multiple signaling cascades, including nuclear factor (NF)-κB. A20 (also known as TNF alpha induced protein 3 or TNFAIP3) has emerged as critical ubiquitin-editing enzyme that functions as an endogenous regulator of inflammation through termination of NF-κB activation as part of a negative feedback loop. Through its function in a plethora of biological processes, aberrant expression and/or function of A20 has been implicated in gastrointestinal, …

Transcriptomic Profiling Of Postmortem Prefrontal Cortex And Nucleus Accumbens From Chronic Alcohol Abusers., Eric S. Vornholt

Theses and Dissertations

Alcohol use disorder (AUD) is a debilitating psychiatric illness that develops from a combination of genetic and environmental factors. While it is well documented that AUD is heritable, the shift from recreational alcohol use to abuse/dependence is poorly understood. In this dissertation, using postmortem brain tissue from individuals with alcohol dependence (AD), we profiled the genome-wide expression of circular RNA (circRNA), microRNA (miRNA), and messenger RNA (mRNA) to better understand the impact of gene expression on the development of AUD. To achieve this, we performed two independent studies that explore transcriptome differences between AD cases and controls. The first of …

Culturally-Relevant Factors That Influence Healthy Eating Among African American College Students, Danyel Smith

Theses and Dissertations

Obesity is a national epidemic in the US, disproportionately affecting African Americans, such that the obesity prevalence in African Americans (49.6%) surpassed the national prevalence (42.4%) in 2018. Those same disparities exist at the collegiate level, such that 27.4% of a sample of African Americans college students had obesity, surpassing the national prevalence of obesity (12.1%) in 2018. Diet, an important driver of obesity, is influenced by several psychological, social, and environmental factors. However, cultural factors influencing diet are understudied among African American college students. The overarching goal of the current study was to identify culturally-relevant factors that promote healthy …

I Hope My Black Skin Don't Dirt This White Tuxedo, Luis A. Vasquez La Roche

Theses and Dissertations

I Hope My Black Skin Don't Dirt This White Tuxedo is a series of works--sculpture, installations, and performances--that explore themes of shame, failure, commodity, ephemerality, ritual, resilience, erasure, race, and death. The research and interest in these themes stem from a page of the Trinidad and Tobago Slave Registry. I use the research that surrounds this document to highlight different moments in history, in my personal life, and to imagine near futures.

The Heat Is On: Comparison Of Thermoregulatory Behavior In Parasitised Versus Unparasitized Caterpillars Of The Tobacco Hornworm, Manduca Sexta (L.) [Lepidoptera: Sphingidae], Jessica M. Johnston

Theses and Dissertations

Insect thermoregulatory research has demonstrated evidence of variation occurring within and among species with respect to infection, pathogens, and parasitism, but few studies have examined differences in thermoregulatory behavior in a host-parasite system with a coevolved symbiotic virus. I investigated the role of parasitism by Cotesia congregata (Say) (Hymenoptera: Braconidae) on the thermoregulatory behavior of Manduca sexta (L.) (Lepidoptera: Sphingidae) using an experimental design in a thermal gradient chamber and a complementary observational field study on wild M. sexta collected from tobacco farms. Parasitism and larval age were not found to have a significant effect on the preferred body temperature …

Anti-Tumor Functions Of Sphingosine Kinase 1 And Sphingosine Kinase 2 In Breast Cancer Development, Melissa A. Maczis

Theses and Dissertations

Bioactive sphingolipid metabolite sphingosine-1‐phosphate (S1P) circulating levels have been implicated in breast cancer (BC) progression. BCs usually respond to 17β-Estradiol (E2) through canonical receptor ERα66 for genomic effects, however, E2 also triggers rapid, non-genomic responses. E2 has been shown to activate sphingosine kinase 1 (SphK1), increasing S1P for S1P receptors signaling important for BC. The E2 receptor activating SphK1 has not been identified. We demonstrate triple negative BC cells, expressing only novel ERα splice variant ERα36, E2-induced SphK1 activation for S1P secretion. Tamoxifen, first-line BC endocrine therapy, an ERα66 antagonist but ERα36 agonist, activates SphK1 and increases S1P secretion in …

Synthesis And The Genetic Encoding Of Novel Lysine Post-Translational Modifications, Sahan A. Galbada Liyanage

Theses and Dissertations

Post-translational modifications (PTMs) play a significant role in regulating cell signaling and regulating the inner cellular environment. The study of PTMs and their primary biological functions is critical for the development of new medicines targeting the pathways and biomolecules responsible for the PTMs.

Previous studies revealed the direct coupling of the β -hydroxybutyryl lysine (K_BHB) modification with the gene expression. K_BHB in histone proteins links metabolism to epigenetics and gene expression and enables to study in-depth, how cell metabolism controls the gene expression and vice versa. Similar novel histone modification, K_Lac, attracts a special interest …

Virginia Dental Journal (Vol. 97, No. 4, 2020)

Virginia Dental Journal

No abstract provided.

Plant-Pollinator Associations In An Eastern Serpentine Savannah And The Effects Of Overbrowsing, Allyson Richins

Theses and Dissertations

Chapter 1: Native plant response to deer overbrowsing in a serpentine savannah

Plants are particularly vulnerable to physical disturbance in low productivity areas, due to a high cost of replacing lost plant tissue. In the eastern United States, serpentine grasslands are fragmented ecosystems with high concentrations of rare endemic plant species, low concentrations of soil nutrients, and uncontrolled deer overpopulation. This study assessed functional responses of native angiosperms in a rare eastern serpentine savannah to selective deer browsing. Plant count, flower count, floral area, vegetative area, and plant height of 10 serpentine plant species were compared inside and outside of …

Augmenting Guadecitabine To Promote Anticancer Immunity In Murine Breast Cancer, Timothy M. Smith Jr.

Theses and Dissertations

In immune-competent individuals, tumor growth occurs due to a failure of the immune system to recognize and destroy malignant cells. Immune surveillance can be curtailed by the presence of immunosuppressive cells such as myeloid-derived suppressor cells (MDSCs) and T-regulatory cells (Tregs) which both serve to dampen antitumor immunity. These studies utilize a metronomic low-dose therapeutic approach to examine the effects of anticancer drugs against murine breast cancer and the effects on the immune cell compartments. The focus of these studies revolve around the drug guadecitabine (guad), a second-generation DNA methyl-transferase inhibitor (DNMTi). DNMTi’s have been shown to dysregulate the methylation …

Enhancing Chemosensitivity Of Lung Cancers By Manipulating The Tumor Microenvironment: Local Lung Delivery And Nanocarrier-Based Therapies, Hanming Zhang

Theses and Dissertations

Cancer is the second leading cause of death in United States causing an estimated six hundred thousand death in 2019. Among different types of cancer, lung cancer is the leading cause of cancer related death in both male and female. While targeted therapy and immunotherapy have shown promising clinical outcomes, currently there remain a significant proportion of patients who do not benefit from these strategies or who cannot tolerate them, making Pt based doublet chemotherapy still an indispensable component in almost all stages of treatment. However, partially due to the poor tumor distribution of small molecule drug, the development of …

Game-Theoretical Model Of Retroactive Hepatitis B Vaccination In China, Vooha Putalapattu

UROP Posters

Game-Theoretical Model of Retroactive Hepatitis B Vaccination in China

Vooha Putalapattu, Depts. of Psychology, Chemistry, & Biology, with Dr. Jan Rychtar, Dept. of Mathematics

Hepatitis B (HepB) is one of the most common infectious diseases affecting over two billion people worldwide. About one third of all HepB cases are in China. In recent years, China made significant efforts to implement a nationwide HepB vaccination program and reduced the number of unvaccinated infants from 30% to 10%. However, many individuals still remain unprotected, particularly those born before 2003. Consequently, a catch-up retroactive vaccination is an important and especially cost-effective way to …

Symmetry Algebras Of The Canonical Lie Group Geodesic Equations In Dimension Five, Hassan Almusawa

Theses and Dissertations

Nowadays, there is much interest in constructing exact analytical solutions of differential equations using Lie symmetry methods. Lie devised the method in the 1880s. These methods were substantially developed utilizing modern mathematical language in the 1960s and 1970s by several different groups of authors such as L.V. Ovsiannikov, G. Bluman, and P. J. Olver, and have since been implemented as a software package for symbolic computation on commonly used platforms such as Mathematica and MAPLE.

In this work, we first develop an algorithmic scheme using the MAPLE platform to perform a Lie symmetry algebra identification and validate it on nonlinear …

A Theoretically-Based Mixed Methods Approach To Examining Mental Health Disorders And Help-Seeking Behaviors Among University-Enrolled African American Men, Kofoworola D.A. Williams

Theses and Dissertations

Recent studies show that African American men in college are disproportionately experiencing high levels of psychological distress, increasing their risk of developing mental health symptoms associated with depression and anxiety. Despite being at higher risk for developing mental health symptoms, university-enrolled, African American men are not seeking help even when experiencing high levels of stress and anxiety, impacting their academic performance. The evidence base describing mental health symptom prevalence and evidence-based prevention strategies among university-enrolled, African American men is limited. To that end, this dissertation work utilized a concurrent, mixed methods approach, theoretically-based in the Social Ecological Model (SEM), to …

Examining Body Mass Index And Sepsis Mortality At One Year After Sepsis, Jamie D. Robinson

Theses and Dissertations

Introduction: Sepsis is a serious and life-threatening syndrome affecting 1.7 million Americans annually and resulting in approximately 270,000 deaths. An “obesity paradox” where obese individuals have lower sepsis mortality than their non-obese counterparts has been described. The problem is that the longevity of the effect is unknown, and few studies have examined the obesity paradox after 1-2 months post-diagnosis.

Methods: This retrospective cohort study examined clinical, demographic, and biomarker variables thought to affect sepsis mortality at three-time points: 30 days, 180 days, and at one year post-sepsis diagnosis in order to shed light on specific factors that might define …

Reaction Profiling Of Extracellular Protein Phosphorylation Through A Microfluidic Reactor Coupled With Raman Spectroscopy, Abigail Casey

Theses and Dissertations

Diseases and disorders in the human body are considered abnormalities of proper cellular function. Understanding the signal transduction mechanisms that cause these abnormalities is crucial to developing earlier detection methods, better treatment options and effective cures. While current diagnostic procedures are powerful tools in diagnosing diseases, they are ineffective in informing physicians on the real-time behavior of the signal transduction mechanisms associated with diseases and disorders. Currently, disease progression is monitored over time through routine patient visits and testing by one or more of the above techniques. By developing an approach that can monitor structural and conformational changes of proteins …

Characterization Of The Gonococcal Calprotectin Zn-Acquisition System, Tdfh, And The Efficacy Of Metal Starvation For Treating Gonococcal Disease, Michael T. Kammerman

Theses and Dissertations

Abstract

Characterization of the Gonococcal Calprotectin Zn-Acquisition System, TdfH, and the Efficacy of Metal Starvation for Treating Gonococcal Disease.

By Michael T. Kammerman, B.S.

A dissertation submitted in partial fulfillment of the requirements for the degree of Doctor of Philosophy at Virginia Commonwealth University

Virginia Commonwealth University, 2020

Major Director: Cynthia Nau Cornelissen, Ph.D.

Professor, Department of Microbiology and Immunology

Neisseria gonorrhoeae, the bacterial agent that is responsible for the human disease gonorrhea, has had a steady increase in the number of infections per year. In 2018 the WHO estimated over 87 million infections occurred world-wide, and the CDC …

The Impact Of Aging And Mechanical Injury On Alveolar Epithelial And Macrophage Responses In Acute Lung Injury And Inflammation, Michael S. Valentine

Theses and Dissertations

Patients with severe lung pathologies, such as Acute Respiratory Distress Syndrome (ARDS), often require mechanical ventilation as a clinical intervention; however, this procedure frequently exacerbates the original pulmonary issue and produces an exaggerated inflammatory response that potentially leads to sepsis, multisystem organ failure, and mortality. This acute lung injury (ALI) condition has been termed Ventilator-Induced Lung Injury (VILI). Alveolar overdistension, cyclic atelectasis, and biotrauma are the primary injury mechanisms in VILI that lead to the loss of alveolar barrier integrity and pulmonary inflammation. Stress and strains during mechanical ventilation are believed to initiate alveolar epithelial mechanotransduction signaling mechanisms that contribute …

Novel Bayesian Techniques For Dose Response Data, Faten Alamri

Theses and Dissertations

All forms of life are being exposed to different levels of harmful chemicals that can cause various and serious side effects. Toxicological experiments enable researchers to test these chemicals on animal to determine their major effects. Although optimal and acceptable dose levels have been investigated, researchers continue to strive to minimize side effects and the chemical dosages. Dose-response models and other benchmark approaches play a role in determining the acceptable exposure levels of hazardous chemicals. Parametric techniques, used to determine the tolerable dosages, utilizing ANOVA and non-linear regression models are well represented in the literature. We determined the benchmark dose …

Development Of A Screening Assay For Type Iii Secretion System Inhibitors And High Throughput Screening Campaign Of Inhibitors Of Prp Of Staphylococcus Aureus, Heather A. Pendergrass

Theses and Dissertations

Antibiotics inhibit the growth or survival of bacteria by targeting their essential functions.¹ Due to weaknesses in traditional antibiotics and the increasing prevalence of antibiotic resistance genes, virulence factors are being targeted for therapeutic treatment of bacterial infection.² We have developed an assay to quantify and observe type III secretion system (T3SS) activity. The type III secretion system (T3SS) is a virulence factor present in some Gram-negative pathogens including enteropathogenic and enterohemorrhagic E. coli (EPEC and EHEC, respectively),³ and others.^4–9 The T3SS between EPEC and EHEC are highly conserved and share over 90% sequence identity with …

Understanding The Role Of Macrophages In Lung Inflammation Through Mathematical Modeling, Sarah B. Minucci

Biology and Medicine Through Mathematics Conference

No abstract provided.

Ebola: Impact Of Hospital's Admission Policy In An Overwhelmed Scenario, Mondal H. Zahid

Biology and Medicine Through Mathematics Conference

No abstract provided.

A Study Of Pair Formation Disease Models With A Two Phase Infection, Berlinda Rosa Batista

Biology and Medicine Through Mathematics Conference

No abstract provided.

Living With Hiv After Release From Prison: An Evaluation Of The Long-Term Health Of Formerly Incarcerated Individuals Who Used Michigan’S Community Reentry Service, Robin L. Miller Phd, Danielle Chiaramonte, Miles Mcnall, Jason Forney, Patrick Janulis

Journal of Prison Education and Reentry (2014-2023)

In 2003, Michigan implemented a reentry service to assist HIV-infected people incarcerated in state prisons in linking to HIV medical care immediately upon their release. We examined whether formerly incarcerated people were linked to care successfully, remained in care, and were in good health 3 years after their date of release. In all, 190 people used the service over the 5 years following its inception. Only a minority of those who were alive and not reincarcerated at the time of the evaluation engaged consistently with medical care. Unsurprisingly given low rates of engagement in care, 3 years after their release …

A Systematic Review Of Cases Of Meningitis In The Absence Of Cerebrospinal Fluid Pleocytosis On Lumbar Puncture, Michelle Troendle, Alexis Pettigrew

Emergency Medicine Publications

Background: Definitive diagnosis of meningitis is made by analysis of cerebrospinal fluid (CSF) culture or polymerase chain reaction (PCR) obtained from a lumbar puncture (LP), which may take days. A timelier diagnostic clue of meningitis is pleocytosis on CSF analysis. However, meningitis may occur in the absence of pleocytosis on CSF.

Areas of Uncertainty: A diagnosis of meningitis seems less likely without pleocytosis on CSF, leading clinicians to prematurely exclude this. Further, there is little available literature on the subject.

Methods: Ovid/Medline and Google Scholar search was conducted for cases of CSF culture-confirmed meningitis with lack of pleocytosis. …

Setting Foundations For Pediatric Fellowship Core Curriculum Training, Ashlie Tseng

Health Sciences Education Symposium

Purpose and background:

The American Board of Pediatrics (ABP) and the Accreditation Council for Graduate Medical Education (ACGME) require pediatric subspecialty fellows to learn a core curriculum of scholarly topics including biostatistics, research methodology, teaching, quality improvement and leadership. Traditionally, each fellowship would develop such curricula for their own fellows –leading to variable experiences for fellows in different disciplines. Nationally, many fellowships are working to provide a common core curriculum which is available across disciplines with an aim towards improving training and transitioning to academic careers.1,2,3 To address this, we have developed a core curriculum which aims to provide all …

Virginia Commonwealth University Commencement Program [2019], Virginia Commonwealth University

VCU Commencement Programs

No abstract provided.

Development Of Bivalent Ligands Targeting The Putative Mu Opioid Receptor And Chemokine Receptor Cxcr4 Heterodimer, Bethany A. Reinecke

Theses and Dissertations

Human immunodeficiency virus (HIV) and opioid abuse have been described as synergistic epidemics. Pharmacologically, it has been found that opioids have the capacity to enhance HIV infection and replication. Research has shown that activation of the mu-opioid receptor (MOR) elevates the expression of the HIV-1 entry co-receptor CXCR4 on T-lymphocytes in the peripheral nervous system, thus allowing for enhanced viral entry and invasion. Although the exact mechanism for opioid modulation of CXCR4 expression and subsequent exacerbation of HIV is unknown, several hypotheses exist. One hypothesis is that MOR and CXCR4 are functionally interacting through the formation of a heterodimer. This …