Digital Commons Network^™

Power Analysis In Applied Linear Regression For Cell Type-Specific Differential Expression Detection, Edmund Glass

Theses and Dissertations

The goal of many human disease-oriented studies is to detect molecular mechanisms different between healthy controls and patients. Yet, commonly used gene expression measurements from any tissues suffer from variability of cell composition. This variability hinders the detection of differentially expressed genes and is often ignored. However, this variability may actually be advantageous, as heterogeneous gene expression measurements coupled with cell counts may provide deeper insights into the gene expression differences on the cell type-specific level. Published computational methods use linear regression to estimate cell type-specific differential expression. Yet, they do not consider many artifacts hidden in high-dimensional gene expression …

Genomic Comparisons And Genome Architecture Of Divergent Trypanosoma Species, Katie Bradwell

Theses and Dissertations

Virulent Trypanosoma cruzi, and the non-pathogenic Trypanosoma conorhini and Trypanosoma rangeli are protozoan parasites with divergent lifestyles. T. cruzi and T. rangeli are endemic to Latin America, whereas T. conorhini is tropicopolitan. Reduviid bug vectors spread these parasites to mammalian hosts, within which T. rangeli and T. conorhini replicate extracellularly, while T. cruzi has intracellular stages. Firstly, this work compares the genomes of these parasites to understand their differing phenotypes. Secondly, genome architecture of T. cruzi is examined to address the effect of a complex hybridization history, polycistronic transcription, and genome plasticity on this organism, and study its highly …

Reciprocal Relations Between Traumatic Stress And Physical Aggression During Middle School, Erin L. Thompson

Theses and Dissertations

There is convincing evidence that demonstrates traumatic stress and aggressive behavior are highly related among adolescents. The evidence is less clear regarding the direction of this relation. The purpose of this study was to examine the reciprocal longitudinal relations between physical aggression and traumatic stress among a predominantly African American sample of middle school students. Support was found for traumatic stress predicting increased levels of physical aggression across the winter to the spring of the sixth grade for boys and across all waves from the fall of the seventh grade to the fall of the eighth grade for both boys …

A Computational Study Of Curvature In The Outflow Graft Of A Continuous Flow Left Ventricular Assist Device, Laura Patterson

Theses and Dissertations

Left ventricular assist devices (LVADs) are an increasingly utilized therapy for end-stage heart failure. Thrombosis within the graft from the pump to the aorta has been documented, but is poorly researched. This study examines the effect of graft geometry, as measured by radius of curvature, bend angle, and diameter, on thrombogenic flow patterns within the graft for a range of flow conditions. It also examines the effect blood properties, including viscosity and density, on these flow patterns. The results indicated that radius of curvature had a powerful effect on thrombogenic flow patterns. Flowrate and bend angle were also influential. The …

Determinants Of Active Pursuit Of Kidney Donation: Applying The Theory Of Motivated Information Management, Stacy M. West

Theses and Dissertations

End stage renal disease (ESRD) is a growing epidemic impacting the United States. While the optimal treatment for ESRD is renal replacement, barriers exist making this treatment difficult and sometimes impossible for patients to pursue. One potential solution to existing barriers is to encourage patients to actively seek living donors. This is an inherently communicative and social process. The Theory of Motivated Information Management (TMIM) offers a framework for understanding factors that contribute to patients’ conversations about transplantation with their social networks. It is also possible that Patient Empowerment can add to this model, and inform future patient education.

Specific …

Pathways Linking Sleep Timing To Obesity In Midlife Women, Dana R. Schreiber

Theses and Dissertations

Midlife women are vulnerable to developing obesity. Both sleep timing and negative emotion are risk factors, yet limited research has explored their role on weight outcomes in this population. The current investigation explored the association of sleep timing (i.e., mean sleep time, sleep time variability) and obesity (i.e., BMI, waist circumference) in midlife women, considering emotional pathways (i.e., depressive symptoms, anger) that might mediate this association. PROCESS parallel mediation models assessed direct and indirect pathways. In follow-up analyses, sleep duration was explored as an indirect pathway linking sleep timing to obesity. Results demonstrated that sleep timing does not directly predict …

Supporting Teen Leaders: Validation Of The I Drive Smart Survey, Cynthia M. George

Theses and Dissertations

Recent policy change allows states to spend federal dollars directly on teen-led driver safety efforts and requires regular evaluations of effectiveness. There are currently no standardized instruments to measure change in teen driving behavior relevant to teen leaders. This study serves the Tennessee Department of Safety and Homeland Security, Tennessee Highway Safety Office and their network of teen leaders to empirically test and refine the I Drive Smart survey developed by partners and grounded in the Theory of Planned Behavior (TPB). The survey is designed to be administered by teen leaders to their peers and produce data relevant for use …

Longitudinal Relations Between Parental And Peer Support For Violent And Nonviolent Responses To Conflict And Early Adolescent Dating Aggression, Rachel C. Garthe

Theses and Dissertations

High prevalence and the negative legal, health, and psychological consequences of adolescent dating aggression underscore the need to identify risk and protective processes associated with this type of aggression. Studying dating aggression in early adolescence is important, as this is the developmental time frame when most youth are establishing attitudes, beliefs, and norms for dating behaviors. The current study investigated longitudinal associations between perceived parental and peer support for violent and nonviolent responses to conflict and dating aggression perpetration among middle school students. Participants included 1,399 adolescents (52% female) in the sixth (n = 466), seventh (n = …

The Effects Of Amixicile, A Pyruvate Ferredoxin Oxidoreductase Inhibitor, On Oral Treponemes, Lucas A. Reed

Theses and Dissertations

Periodontal disease (PD) is a polymicrobial infection characterized by inflammation of the gingiva, alveolar bone resorption, and tooth loss (edentulism). Treponema denticola along with Porphyromonas gingivalis and Tannerella forsythia are among the “Red Complex” and are main etiological agents in PD. Treponemes are a member of the Spirochaeta phylum and are obligate anaerobes, that express pyruvate ferredoxin oxidoreductase (PFOR). The enzyme catalyzes the oxidation of pyruvate to acetyl-CoA and reduced ferredoxin. Amixicile is a novel bacteriostatic derivative of nitazoxanide and an inhibitor of PFOR. In light of the fact that Treponemes express PFOR, this study was conducted to investigate the …

Positive Illusory Bias In Adolescents With Adhd: Prevalence, Stability, And Accuracy Of Reporters, Elizaveta Bourchtein

Theses and Dissertations

The positive illusory bias (PIB) – over-reporting levels of self-competence compared to other raters – has been demonstrated in youth with attention-deficit/hyperactivity disorder (ADHD), despite increased impairment rates. However, due to inconsistent definitions of the PIB, conflicting findings have emerged regarding prevalence of the PIB in youth with ADHD and whether parent or child report is actually “biased” and driving the PIB. Additionally, stability of the PIB across time is unknown. The present study used person-centered methodology cross-sectionally and longitudinally to evaluate the prevalence of the PIB in adolescents with ADHD. Results revealed a cross-domain global PIB group at baseline …

Investigation Of Anaplasma Phagocytophilum And Anaplasma Marginale Adhesin-Host Cell Interactions, Kathryn S. Hebert

Theses and Dissertations

Anaplasma phagocytophilum and A. marginale are the etiologic agents of bovine anaplasmosis and human granulocytic anaplasmosis, respectively. As obligate intracellular pathogens, binding and entry of host cells is a prerequisite for survival. The molecular events associated with these processes are poorly understood. Identifying the adhesins mediating binding, delineating their key functional domains, and determining the molecular determinants to which they bind not only benefits better understanding of Anaplasma spp. pathobiology, but could also benefit the development of novel approaches for protecting against infection. We previously demonstrated that A. phagocytophilum outer membrane protein A (ApOmpA) is critical for bacterial binding and …

Assembly And Regulation Of The Dream Complex, Jessica G. Felthousen

Theses and Dissertations

The DREAM complex assembles during G0/G1 when RB-like protein p130 recruits E2F4, DP1, and a core complex of five MuvB proteins to repress genes involved in cell cycle progression. In S-phase, the MuvB core dissociates from p130 and binds to BMYB transcription factor. Binding of the MuvB core to p130 requires phosphorylation of its subunit LIN52 at S28 residue by DYRK1A protein kinase. However, little is known about how the MuvB core interacts with p130 to form the DREAM complex, and how these interactions are manipulated throughout the cell cycle. In collaboration with Dr. Seth Rubin, we characterized the structural …

The Effects Of 7,8-Dihydroxyflavone On Hippocampal Neurogenesis Following Traumatic Brain Injury, Mary K. Wurzelmann

Theses and Dissertations

Following traumatic brain injury (TBI), the hippocampus is particularly vulnerable to damage, and BDNF, an endogenous neurotrophin that activates the TrkB receptor, has been shown to play a key role in the brain’s neuroprotective response. Activation of the TrkB signaling pathway by BDNF in the CNS promotes cell survival and aids in cell growth. However, due to its inability to cross the blood brain barrier (BBB), the therapeutic advantages of BDNF treatment following TBI are limited. 7,8-Dihydroxyflavone (7,8-DHF) is a flavonoid that mimics the effects of BDNF, is a potent TrkB receptor agonist, and can successfully cross the BBB. Our …

Characterizing The Oncogenic Properties Of C-Terminal Binding Protein, Evan T. Sumner

Theses and Dissertations

The paralogous C-terminal binding proteins (CtBP) 1 and 2 are evolutionarily conserved transcriptional coregulators that target and disrupt the expression of several genes essential for multiple cellular processes critical to regulating tumor formation. CtBP’s ability to govern the transcription of genes necessary for apoptosis, tumor suppression, invasion/migration and EMT gives rise to its oncogenic activities. Both isoforms of CtBP are found to be overexpressed in cancers including colorectal, pancreatic, ovarian, and breast, with higher levels correlating to lower overall median survival. Although multiple lines of evidence suggest CtBP plays a role in tumorigenesis, it has never been formally characterized as …

Sciatic Peripheral Nerve Blockade For Pain Control Following Hamstring Autograft Harvest In Adolescents: A Comparison Of Two Techniques, James Furstein

Theses and Dissertations

Anterior cruciate ligament reconstruction utilizing a hamstring autograft is a surgical technique that has gained popularity among orthopedic surgeons caring for adolescent patients. While utilization of a hamstring autograft is a revered technique, harvest of the hamstring yields significant pain. Sciatic peripheral nerve blockade has proven to reliably provide analgesia at the hamstring donor site. Single-injection sciatic peripheral nerve blockade is considered a basic and effective technique, making its use following anterior cruciate ligament reconstruction standard practice in many institutions. The duration of action of a single-injection sciatic peripheral nerve blockade may fail to outlast the pain arising from the …

Trabecular Bone Microarchitecture In Association With Systemic And Local Factors As Potential Predictors Of Implant Failure, Diego A. Camacho Dmd

Theses and Dissertations

Clinicians have described the success rates of dental implants. The use of implants is projected to increase in the future. With a 5-10% failure rate, it is unclear the exact factors that are associated with implant failures. To improve upon these success rates, it is critical to understand parameters associated with implant failure including: periodontitis, peri-implantitis, systemic diseases, site preparation, dental history of the implant site, bone quality, materials, occlusion, and treatment timelines. While bone quality is associated with failure, objective measures to assess bone quality and characteristics are scarce. Therefore, the aim of this study is to determine whether …

Tumor-Specific Cell Death Induction By Noxa Overexpression For Head And Neck Squamous Cell Carcinoma (Hnscc) Treatment, Nicolas T. Maxim Mr.

Theses and Dissertations

The primary focus of this research is the mechanisms of cell death in head and neck squamous cell carcinoma (HNSCC) treatment. These cancers typically originate in squamous cells that line the moist mucosal surfaces of head and neck. HNSCC is commonly treated with a platinum based agent, cisplatin. While the drug does offer strong antitumor effects, its prolonged use often results in tumor-acquired resistance, which limits treatment effectiveness. We have shown that cisplatin treatment induces the expression of a pro-apoptotic BCL-2 family member Noxa, which then initiates caspase- dependent apoptosis through its binding and sequestration of pro-survival protein MCL-1 for …

Para, William Matheson

Theses and Dissertations

We are hosts, we are hosts and we’re deeply aware of it.

We are aware of the pollutants and particulates that exist in our air, aware of the

growing presence of UVB radiation that touches our skin, aware of the microbes,

bacteria and viruses that call our interiors home, more completely aware than we’ve

ever been that to have a body contemporarily is to acknowledge a multiplicity that

can be wholly uncomfortable and even alien: that we are hosts to so much seemingly

unwanted material, sensation and life.

Comfort Toys: Coping Tools For Children With An Epileptic Parent, Benjamin Evjen

Theses and Dissertations

When a caregiver suffers from epilepsy, a child can often feel frightened, vulnerable, and alone whenever a seizure occurs. In my thesis project I intend to help children negotiate this struggle with the use of therapeutic toys. Through play, children can navigate feelings that are often overlooked by adults. The visual appeal, simplicity, and materials used in their creation help facilitate comfort through sensory cues. By applying coping mechanisms to deal with stress caused by the passage of time, provide tactile comfort, and equip the child with tools to take action, their emotional needs are met. These toys address an …

Factors Affecting Institutional Performance At High And Very High Research Universities: Policy Implications, Jose G. Alcaine

Theses and Dissertations

Higher education institutions in the Unites States (U.S.) are under stress. Universities and colleges in the U.S. face competing demands marked by steeply declining state and local appropriations and increased competition for research dollars and prestige. This stress is felt most acutely at high and very high research universities who must face these funding challenges while at the same time must serve a multiplicity of missions and stakeholders.

This study examines factors that influence institutional performance at high and very high research universities in the U.S. These high and very high research universities, as classified by the Carnegie Foundation for …

Modeling Time-Dependent Performance Of Submerged Superhydrophobic Or Slippery Surfaces, Ahmed A. Hemeda

Theses and Dissertations

The goal of this study is to quantify the transient performance of microfabricated superhydrophobic surfaces when used in underwater applications. A mathematical framework is developed and used to predict the stability, longevity, and drag reduction benefits of submerged superhydrophobic surfaces with two- or three-dimensional micro-textures. In addition, a novel design is proposed to improve the drag-reduction benefits of lubricant-infused surfaces, by placing a layer of trapped air underneath the lubricant layer. The new design is referred to as lubricant–infused surfaces with trapped air, and it is designed to eliminate the long-lasting longevity problem of submerged superhydrophobic surfaces. The effectiveness of …

Pharmacological Inhibition Of Cyclophilin Ameliorates Experimental Allergic Encephalomyelitis, Zi L. Huang

Theses and Dissertations

A subset of cyclophilins have been implicated in mechanisms of neuroinflammation and neurodegeneration that contributes to the pathogenesis of Multiple Sclerosis. Mitochondrial dysfunction leading to mitochondrial permeability transition plays a pivotal role in axonal damage and disease progression in Multiple Sclerosis. Cyclophilin D (CypD) is a crucial regulatory component of the mitochondrial permeability transition pore and it was demonstrated that the cyclophilin D knockout animals showed reduced experimental allergic encephalomyelitis (EAE) clinical disease severity and axonal injury. We investigated the effect of N-methyl-4-isoleucine-cyclosporin (NIM811), a non-immunosuppressive and non-selective cyclophilin inhibitor, on the course and severity of EAE. EAE mice treated …

Pushed Without Direction: Privileged Problems And The Configuration Of Class And Race. How Latent Class Differences, Supported Through Racial Inequities, Maintain The Achievement Gap For Upper Class Black Students, Jullian Harrison

Theses and Dissertations

Scholars for decades have studied the achievement gap and attempted to explain it in regards to race and class. Throughout the existing literature regarding the achievement gap between black and white students, however, there is a dearth of research exploring why the gap exists for upper-class black students; this population is largely ignored. This research seeks to explain why an achievement gap exists between white and black students who come from households of similar incomes. Ten students (five white and five black gradates) of a private, non-parochial school in Washington DC are interviewed about high school and post-high school experiences. …

Fuck 2016 (2016)

Amendment, 2004-

A zine companion to the Amendment.

Can Student-Built Gsas Provide Positive Development For Lgbt Youth In High Schools? A Review Of Literature, Beau Coggsdale

AUCTUS: The Journal of Undergraduate Research and Creative Scholarship

Within school systems, there are sexual minority students that are not treated with the same amount of attention that the sexual majority gets. These minority students include individuals that identify as bisexual, gay, lesbian, transgender, or questioning. These students would fall under the category of lesbian, gay, bisexual, transgender/transsexual, queer, questioning, intersex, asexual, or pan/polysexual (LGBTQQIAP). Many LGBT students in high school report feelings of isolation, depression, anxiety, and suicidal ideation (Hong, Espelage, and Kral 885- 886). Many of these students, especially in rural areas, require some form of support within the school system for healthy positive development. Gay Straight …

Feasibility Of Integrating Tripterygium Wilfordii Into Modern Cancer Therapy For Increased Efficacy With Minimal Toxicity, Andy Vo

AUCTUS: The Journal of Undergraduate Research and Creative Scholarship

Cancer is the second leading cause of death in the U.S., and millions of novel cancer cases are being diagnosed each year. While chemotherapy and ionizing radiation are effective treatments against these malignant tumors, the adverse effects that accompany such treatments are devastating. In order to find alternative treatment methods with less side effects, we turn to Eastern herbal medicine. Recent scientific research has found that Tripterygium wilfordii, an herbal medicine traditionally used to treat inflammation in China, contains compounds (triptolide and celastrol) that prevent the growth of solid tumors, induce apoptosis, and prevent metastasis of developed tumors. Investigations on …

Holistic Products: Designing With Time, Gifts, And Ritual, Jeremy P. Zietz

Theses and Dissertations

The notion of “you are what you buy” is an updated adage from “you are what you eat”. It makes a connection between our everyday objects and their effect on our lived experience. Looking at our relationships with our things as a type of contract, we must be intentional to shape these object contracts for our own good and health. Instead of our society’s design talents being put toward a consumerist agenda, designers must direct research and development which addresses the effects of our products holistically.

Various concepts have emerged in my creative practice which demand a deeper research and …

Acute And Chronic Effects Of Inhalants In Intracranial Self-Stimulation, Matthew Tracy

Theses and Dissertations

Inhalants are a loosely defined diverse group of volatile substances which people abuse. Despite widespread misuse of inhalants, there are limited preclinical methods available to study the reinforcement-like properties of inhalants. One procedure which has demonstrated substantial promise as a tool to investigate inhalant pharmacology is the intracranial self-stimulation (ICSS) procedure. ICSS utilizes pulses of electrical stimulation to the mesolimbic reward pathway to serve as a temporally defined and controlled operant reinforcer with a highly adjustable efficacy. The first aim of the project was to characterize the effects of commonly abused inhalants: including toluene, trichloroethane, nitrous oxide, isoflurane and R134a …

Comparison Of Treatment Planning Decisions When Combining Cbct And Digital Radiography Verses Digital Radiography Alone, Mckay Barlow Packer

Theses and Dissertations

Cone beam computed tomography (CBCT) is the recommended imaging modality of choice for evaluating previous endodontic treatment (1). The aim of this study was to compare treatment planning decisions made when evaluating previous endodontic treatment to determine if treatment planned and projected prognosis differs when digital radiography is used alone or in combination with CBCT. A retrospective chart review was conducted. Patients for whom a CBCT was taken were included in the study. Twenty-eight patients qualified. Patient’s periapical digital radiographs (Dexis©) were evaluated by 2 calibrated endodontists, a treatment plan was identified and a prognosis was projected. Later the same …

Understanding Teacher Morale, Jesse Senechal, Tamara Sober, Samantha Hope, Teri Johnson, Felicia Burkhalter, Teri Castelow, Debbie Gilfillan, Kenya Jackson, Autumn Nabors, Patrick Neuman, Rodney Robinson, Rob Sargeant, Stacy Stanford, Deanna Varljen

MERC Publications

This study emerged from discussions within the Policy and Planning Council of the Metropolitan Educational Research Consortium (MERC), a research alliance between Virginia Commonwealth University’s School of Education and seven surrounding school divisions.

The project has two goals. The first goal is to develop an understanding of the factors that impact teachers’ experience of their work in the current PK12 public school context. Although this topic could be, and has been, investigated through a number of lenses (e.g., burnout, trust, motivation), this project focuses on the idea of teacher morale, a choice that will be discussed in detail in the …