Digital Commons Network^™

The Pathogen-Occupied Vacuoles Of Anaplasma Phagocytophilum And Anaplasma Marginale Interact With The Endoplasmic Reticulum, Hilary K. Truchan, Chelsea L. Cockburn, Kathryn S. Hebert, Forgivemore Magunda, Susan M. Noh, Jason A. Carlyon

Microbiology and Immunology Publications

The genus Anaplasma consists of tick-transmitted obligate intracellular bacteria that invade white or red blood cells to cause debilitating and potentially fatal infections. A. phagocytophilum, a human and veterinary pathogen, infects neutrophils to cause granulocytic anaplasmosis. A. marginale invades bovine erythrocytes. Evidence suggests that both species may also infect endothelial cells in vivo. In mammalian and arthropod host cells, A. phagocytophilum and A. marginale reside in host cell derived pathogen-occupied vacuoles (POVs). While it was recently demonstrated that the A. phagocytophilum-occupied vacuole (ApV) intercepts membrane traffic from the trans-Golgi network, it is unclear if it or …

Involvement Of Nadh Oxidase In Biofilm Formation In Streptococcus Sanguinis, Xiuchun Ge, Xiaoli Shi, Limei Shi, Jinlin Liu, Victoria Stone, Fanxiang Kong, Todd Kitten, Ping Xu

Microbiology and Immunology Publications

Biofilms play important roles in microbial communities and are related to infectious diseases. Here, we report direct evidence that a bacterial nox gene encoding NADH oxidase is involved in biofilm formation. A dramatic reduction in biofilm formation was observed in a Streptococcus sanguinis nox mutant under anaerobic conditions without any decrease in growth. The membrane fluidity of the mutant bacterial cells was found to be decreased and the fatty acid composition altered, with increased palmitic acid and decreased stearic acid and vaccenic acid. Extracellular DNA of the mutant was reduced in abundance and bacterial competence was suppressed. Gene expression analysis …

Interaction Between Cannabinoid System And Toll-Like Receptors Controls Inflammation, Kathleen L. Mccoy

Microbiology and Immunology Publications

Since the discovery of the endocannabinoid system consisting of cannabinoid receptors, endogenous ligands, and biosynthetic and metabolizing enzymes, interest has been renewed in investigating the promise of cannabinoids as therapeutic agents. Abundant evidence indicates that cannabinoids modulate immune responses. An inflammatory response is triggered when innate immune cells receive a danger signal provided by pathogen- or damage-associated molecular patterns engaging pattern-recognition receptors. Toll-like receptor family members are prominent pattern-recognition receptors expressed on innate immune cells. Cannabinoids suppress Toll-like receptor-mediated inflammatory responses. However, the relationship between the endocannabinoid system and innate immune system may not be one-sided. Innate immune cells express …

Interaction Between Cannabinoid System And Toll-Like Receptors Controls Inflammation, Kathleen L. Mccoy

Microbiology and Immunology Publications

Since the discovery of the endocannabinoid system consisting of cannabinoid receptors, endogenous ligands, and biosynthetic and metabolizing enzymes, interest has been renewed in investigating the promise of cannabinoids as therapeutic agents. Abundant evidence indicates that cannabinoids modulate immune responses. An inflammatory response is triggered when innate immune cells receive a danger signal provided by pathogen- or damage-associated molecular patterns engaging pattern-recognition receptors. Toll-like receptor family members are prominent pattern-recognition receptors expressed on innate immune cells. Cannabinoids suppress Toll-like receptor-mediated inflammatory responses. However, the relationship between the endocannabinoid system and innate immune system may not be one-sided. Innate immune cells express …

Clinically Relevant Investigation Of Flattening Filter-Free Skin Dose, Christopher L. Guy, Kishor Karki, Manju Sharma, Siyong Kim

Radiation Oncology Publications

As flattening filter-free (FFF) photon beams become readily available for treatment delivery in techniques such as SBRT, thorough investigation of skin dose from FFF photon beams is necessary under clinically relevant conditions. Using a parallel-plate PTW Markus chamber placed in a custom water-equivalent phantom, surface-dose measurements were taken at 2 × 2, 3 × 3, 4 × 4, 6 × 6, 8 × 8, 10 × 10, 20 × 20, and 30 × 30 cm2 field sizes, at 80, 90, and 100 cm source-to-surface distances (SSDs), and with fields defined by jaws and multileaf collimator (MLC) using multiple beam energies …

Virginia Commonwealth University Commencement Program [2016], Virginia Commonwealth University

VCU Commencement Programs

No abstract provided.

Unmanned Aerial Vehicle For Vertically Profiling Ultrafine Particle Distribution, Ahmed Almutawa, Ola Saadeh, Mudaher Alkhawajah, Grant Carey

Capstone Design Expo Posters

Recent epidemiologic studies show that ultrafine particles (UFPs) may play a definitive role in development of pulmonary and cardiovascular diseases, as well as cancer related mortality. In order to support the growing interest in understanding the effects of UFPs on human health, Dr. Chen has developed a miniaturized electronic ultrafine particle sizing device, or mini-eUPS. This device utilizes many of the functions of equipment currently used in laboratory environments to test and measure UFP levels in aerosols, but has been miniaturized to maximize mobility and ease of use. The small size and weight of the device allow a unique ability …

Virginia Commonwealth University Courses [2016], Virginia Commonwealth University

VCU Bulletins

Listing of courses for the 2016-2017 year.

Allosteric Inhibition Of Factor Xiiia. Non-Saccharide Glycosaminoglycan Mimetics, But Not Glycosaminoglycans, Exhibit Promising Inhibition Profile, Rami A. Al-Horani, Rajesh Karuturi, Michael Lee, Daniel K. Afosa, Umesh R. Desai

Medicinal Chemistry Publications

Factor XIIIa (FXIIIa) is a transglutaminase that catalyzes the last step in the coagulation process. Orthostery is the only approach that has been exploited to design FXIIIa inhibitors. Yet, allosteric inhibition of FXIIIa is a paradigm that may offer a key advantage of controlled inhibition over orthosteric inhibition. Such an approach is likely to lead to novel FXIIIa inhibitors that do not carry bleeding risks. We reasoned that targeting a collection of basic amino acid residues distant from FXIIIa’s active site by using sulfated glycosaminoglycans (GAGs) or non-saccharide GAG mimetics (NSGMs) would lead to the discovery of the first allosteric …

The Interactome Of Streptococcus Pneumoniae And Its Bacteriophages Show Highly Specific Patterns Of Interactions Among Bacteria And Their Phages, Rachelle Mariano, Stefan Wuchty, Maria G. Vizoso-Pinto, Roman Häuser, Peter Uetz

Study of Biological Complexity Publications

Although an abundance of bacteriophages exists, little is known about interactions between their proteins and those of their bacterial hosts. Here, we experimentally determined the phage-host interactomes of the phages Dp-1 and Cp-1 and their underlying protein interaction network in the host Streptococcus pneumoniae. We compared our results to the interaction patterns of E. coli phages lambda and T7. Dp-1 and Cp-1 target highly connected host proteins, occupy central network positions, and reach many protein clusters through the interactions of their targets. In turn, lambda and T7 targets cluster to conserved and essential proteins in E. coli, while such …

Network Topology Analysis Of Post-Mortem Brain Microarrays Identifies More Alzheimer’S Related Genes And Micrornas And Points To Novel Routes For Fighting With The Disease, Sreedevi Chandrasekaran, Danail Bonchev

Study of Biological Complexity Publications

Network-based approaches are powerful and beneficial tools to study complex systems in their entirety, elucidating the essential factors that turn the multitude of individual elements into a functional system. In this study we used critical network topology descriptors and guilt-by-association rule to explore and understand the significant molecular players, drug targets and underlying biological mechanisms of Alzheimer’s disease. Analyzing two post-mortem brain gene microarrays (GSE4757 and GSE28146) with Pathway Studio software package we constructed and analyzed a set of protein-protein interaction, as well as miRNA-target networks. In a 4-step procedure the expression datasets were normalized using Robust Multi-array Average approach, …

Effects Of Inositol(S) In Women With Pcos: A Systematic Review Of Randomized Controlled Trials, Vittorio Unfer, John E. Nestler, Zdravko A. Kamenov, Nikos Prapas, Fabio Facchinetti

Obstetrics and Gynecology Publications

Polycystic ovary syndrome (PCOS) is a common endocrine disorder, with complex etiology and pathophysiology, which remains poorly understood. It affects about 5–10% of women of reproductive age who typically suffer from obesity, hyperandrogenism, ovarian dysfunction, and menstrual irregularity. Indeed, PCOS is the most common cause of anovulatory infertility in industrialized nations, and it is associated with insulin resistance, type 2 diabetes mellitus, and increased cardiovascular risk. Although insulin resistance is not included as a criterion for diagnosis, it is a critical pathological condition of PCOS. The purpose of this systematic review is the analysis of recent randomized clinical trials of …

Virginia Dental Journal (Vol. 93, No. 4, 2016)

Virginia Dental Journal

No abstract provided.

Method May Revolutionize Athletic Training, Shawn Scornaienchi

The Scope

Speed is the key to success in track, football and most other sports. Researchers at VCU have found that speed is crucial to success in weight room training as well. They are advocating a new method called velocity-based training, which measures how fast an athlete can lift weights.

Researcher Honored For ‘Outstanding Early Career’, Chris Rubis

The Scope

Dr. Matthew L. Banks, an assistant professor in VCU’s Department of Pharmacology and Toxicology, recently received the university’s Outstanding Early Career Faculty Award. His research centers on two themes – developing behavioral and pharmacological strategies to treat drug addiction, and making existing medications more effective with fewer side effects.

Perseverance Pays Off For Ph.D. Student, Jacob Jaminet

The Scope

Lauren Griggs, a Ph.D. student in biomedical engineering, is working to increase the number of women and minorities entering the STEM disciplines – science, technology, engineering and mathematics. She was recently awarded VCU’s Susan E. Kennedy Award, which recognizes graduate students who are advancing the presence of women in education.

Virginia Commonwealth University Professional Bulletin [2016], Virginia Commonwealth University

VCU Bulletins

Professional programs bulletin for Virginia Commonwealth University for the academic year 2016-2017. It includes information on academic regulations, degree requirements, course offerings, faculty, academic calendar, and tuition and expenses for graduate programs.

Virginia Commonwealth University Graduate Bulletin [2016], Virginia Commonwealth University

VCU Bulletins

Graduate bulletin for Virginia Commonwealth University for the academic year 2016-2017. It includes information on academic regulations, degree requirements, course offerings, faculty, academic calendar, and tuition and expenses for graduate programs.

Researchers Head South To Study Mangroves And Birds, Joe Johnson

The Scope

For two weeks in January, VCU researchers Cathy Viverette and Edward Crawford will accompany 18 students to Panama, where they will study mangrove conservation and avian field ecology.

A 10-Year Study Of Factors Associated With Alcohol Treatment Use And Non-Use In A U.S. Population Sample, Karen G. Chartier, Kierste Miller, T. Robert Harris, Raul Caetano

Social Work Publications

Background

This study seeks to identify changes in perceived barriers to alcohol treatment and predictors of treatment use between 1991–92 and 2001–02, to potentially help understand reported reductions in treatment use at this time. Social, economic, and health trends during these 10 years provide a context for the study.

Methods

Subjects were Whites, Blacks, and Hispanics. The data were from the National Longitudinal Alcohol Epidemiologic Survey (NLAES) and the National Epidemiologic Survey on Alcohol and Related Conditions (NESARC). We conducted two analyses that compared the surveys on: 1) perceived treatment barriers for subjects who thought they should get help for …

Greenhouse Gas Emissions Over A Tidal Cycle In A Freshwater Wetland, Joseph C. Morina, Rima B. Franklin

Rice Rivers Center Research Symposium

Tidal freshwater wetlands are located at the interface of non-tidal freshwater riverine systems and estuarine tidal systems. These habitats experience freshwater tides, creating unique redoximorphic soil characteristics while simultaneously presenting an opportunity for hydrologic nutrient transport into the system. Because of this periodic flooding and draining, tidal freshwater wetlands are systems of intense biogeochemical transformations, which are microbially mediated. Several microbial transformations (e.g., methanogenesis, incomplete denitrification, and nitrification) result in the production of greenhouse gases (CO2, CH4, and N2O) at globally-significant levels. For example, wetlands are one of the greatest sources of methane on Earth, accounting for 20-33% of the …

Medical Literary Messenger (Vol. 3, No. 2, Spring/Summer 2016)

Medical Literary Messenger

Exposure / Dan Campion -- The Second Floor Shop B / Hil Scott -- Incubator Memory / Meridian Johnson -- Take Me to Richmond / Derick Nelson Jenkins -- Destination Unknown / Derick Nelson Jenkins -- Symmetry / Derick Nelson Jenkins -- The Aide / Erika D. Price -- Subconscious / David Yoffe -- What My Uncle is Thinking / Tamsyn Brennan -- Progression / E. F. Schraeder -- Waiting / Justin Nicholes -- Only 1,482 Steps From the Massey Cancer Center Entrance / Dodge Havens -- At Hardy-Owen Funeral Home, Conveniently Located on Dixie Highway / Monique Kluczykowski -- …

Medical Literary Messenger (Vol. 4, No. 1, Fall/Winter 2016)

Medical Literary Messenger

Irene—London, December 2006 / Katharine Harer -- Today / Antonia Lewandowski -- The Bench / Richard LeBlond -- illustration / Alana Barrett -- After Reading Elizabeth Bishop’s “In the Waiting Room” / Nina Bannett -- How to Open Your Eyes / Angus Woodward -- Stage 3 / Amanda Gomez -- NeuroSkull / Alana Barrett -- It / Grace Fryberger -- The Mammogram, Part II / Katharine Harer -- Old and New / Derick Nelson Jenkins -- The Innoculation / Simon Friedman -- illustrations / Alana Barrett -- Shocking / Phyllis Price -- Boutique Hotel / Lynn Ciesielski -- A Sleeping …

Iv Pole For Cardiac Surgery, Sindhu Marampudi, Natalie Noll, Madison Nowak, Rachel Wilbur

Capstone Design Expo Posters

IV lines and pumps need to be transported from the operating room to patient recovery with the patient after cardiac procedures. During this process, the weight of the IV pumps and the small wheels on the IV pole can cause the IV pole to become unbalanced and fall or bend due to the load. Due to a decrease in slack in IV lines to minimize IV line tangling, when an IV pole falls, this can result in the IV lines being torn out of the patient that can lead to blood loss and other injuries. In addition, IV bags and …

Mir-9 Acts As An Oncomir In Prostate Cancer Through Multiple Pathways That Drive Tumour Progression And Metastasis, S. J. Seashols-Williams, W. Budd, G. C. Clark, Q. Wu, R. Daniel, E. Dragoescu, Z. E. Zehner

Biochemistry and Molecular Biology Publications

Identification of dysregulated microRNAs (miRNAs) in prostate cancer is critical not only for diagnosis, but also differentiation between the aggressive and indolent forms of the disease. miR-9 was identified as an oncomiR through both miRNA panel RT-qPCR as well as high-throughput sequencing analysis of the human P69 prostate cell line as compared to its highly tumorigenic and metastatic subline M12, and found to be consistently upregulated in other prostate cell lines including DU-145 and PC3. While miR-9 has been characterized as dysregulated either as an oncomiR or tumour suppressor in a variety of other cancers including breast, ovarian, and nasopharyngeal …

Virginia Commonwealth University Undergraduate Bulletin [2016], Virginia Commonwealth University

VCU Bulletins

Undergraduate bulletin for Virginia Commonwealth University for the academic year 2016-2017. It includes information on academic regulations, degree requirements, course offerings, faculty, academic calendar, and tuition and expenses for undergraduate programs.

Exploration Of Bivalent Ligands Targeting Putative Mu Opioid Receptor And Chemokine Receptor Ccr5 Dimerization, Christopher K. Arnatt, Bethany A. Falls, Yunyun Yuan, Thomas J. Raborg, Ruturaj R. Masvekar, Nazira El-Hage, Dana E. Selley, Anthony V. Nicola, Pamela E. Knapp, Kurt F. Hauser, Yan Zhang

Medicinal Chemistry Publications

Modern antiretroviral therapies have provided HIV-1 infected patients longer lifespans and better quality of life. However, several neurological complications are now being seen in these patients due to HIV-1 associated injury of neurons by infected microglia and astrocytes. In addition, these effects can be further exacerbated with opiate use and abuse. One possible mechanism for such potentiation effects of opiates is the interaction of the mu opioid receptor (MOR) with the chemokine receptor CCR5 (CCR5), a known HIV-1 co-receptor, to form MOR-CCR5 heterodimer. In an attempt to understand this putative interaction and its relevance to neuroAIDS, we designed and synthesized …

Speaking Up: Using Ostes To Understand How Medical Students Address Professionalism Lapses, Constance R. Tucker, Beth A. Choby, Andrew Moore, Robert Scott Parker Ii, Benjamin R. Zambetti, Sarah Naids, Jillian Scott, Jennifer Loome, Sierra Gaffney

Publications from the Office of the Dean

Background: Objective-structured teaching encounters (OSTEs) are used across many disciplines to assess teaching ability. The OSTE detailed in this paper assesses 191 fourth-year medical students’ (M4) ability to identify and address lapses in professionalism based on Association of American Medical Colleges’ professionalism competencies. The research questions addressed are:

• How frequently do M4s address professionalism lapses observed during an OSTE?
• What factors influence whether M4s provide feedback when they observe professionalism lapses in an OSTE?

Methods: Standardized patients (SPs) and standardized learners (SLs) were recruited and trained to participate in a standardized encounter with specific cognitive, social, and behavioral …

Bone - Prosthesis Junction For Active Tendon Implants: A Biomechanical Comparison Of Two Fixation Techniques, Imran Khatri, Jennifer S. Wayne Phd

MD Student Summer Research Fellowship Program Posters

End-to-end repair of damaged tendon is not possible in many instances of injury. Staged flexor tendon reconstruction has been proven to be a valuable method of addressing complex flexor tendon injuries when the patient presents late, has failed primary repair, or the injury mandates it (1). Silicone implants have been used for two-stage flexor tendon reconstructions with reports of long-term use as active implants (2,3). Implants have been reported functional approximately one year post-surgery with some still in use up to 25 years after reconstruction (4). This suggests the potential of the implant serving as a permanent prosthesis. The biomechanical …

Family-Centered Rounds And Medical Student Performance On The Nbme Pediatrics Subject (Shelf) Examination: A Retrospective Cohort Study, Tiffany N. Kimbrough, Victor Heh, N. Romesh Wijesooriya, Michael S. Ryan

Pediatrics Publications

Objective

To determine the association between family-centered rounds (FCR) and medical student knowledge acquisition as assessed by the National Board of Medical Examiners (NBME) pediatric subject (shelf) exam.

Methods

A retrospective cohort study was conducted of third-year medical students who graduated from Virginia Commonwealth University School of Medicine between 2009 and 2014. This timeframe represented the transition from ‘traditional’ rounds to FCR on the pediatric inpatient unit. Data collected included demographics, United States Medical Licensing Examination (USMLE) Step 1 and 2 scores, and NBME subject examinations in pediatrics (PSE), medicine (MSE), and surgery (SSE).

Results

Eight hundred and sixteen participants …