Digital Commons Network^™

Commonwealth Times 1972-12-01

Commonwealth Times, 1969-

No abstract provided.

Characterization Of Deoxyribonucleic Acid From Actinomycetes, Lynn William Enquist

Theses and Dissertations

Deoxyribonucleic acid (DNA) analyses were used to assess on a molecular level, the relationships among representatives of the genera Streptomyces, Nocardia and Mycobacterium. The methods developed in this study have been used for routine analysis of DNA from a large number of actinomycetes and have given reliable and reproducible data.

DNA isolated from various actinomycetes was characterized by buoyant density determinations in CsCl from which the mole fraction guanine plus cytosine (GC) content was calculated. All the streptomycete DNA preparations studied had buoyant densities in the range of 1.7287 to 1.7312 g cm^-3 which corresponded to GC compositions of …

A Tissue Culture Perfusion Chamber With A Substratum Of Reconstituted Collagen, Thomas M. Harris

MCV/Q, Medical College of Virginia Quarterly

A tissue culture chamber is described that introduces the concept of a fixed platform within a "perfusion type" chamber. The platform is made of stainless steel mesh covered with a membrane of reconstituted collagen. The collagen membrane serves as the supporting substratum for the cultured tissue and is of such an optical quality as to permit phase-contrast microscopic observation of the tissue. The perfusion chamber is a Sykes-Moore tissue culture chamber modified to twice its normal depth to receive the screen. Cell growth in the experimental chamber is superior to growth in the control chamber both in speed of proliferation …