Digital Commons Network^™

Novel Strategies In Head And Neck Tumor Therapy; Contribution Of Senescence In Therapy Response, And Resistance Development After Cisplatin Treatment, Fereshteh Ahmadinejad

Theses and Dissertations

Therapeutic outcomes achieved in head and neck squamous cell carcinoma (HNSCC) patients by concurrent cisplatin-based chemoradiotherapy initially reflect both tumor regression and tumor stasis. However, local, and distant metastasis and disease relapse as well as cisplatin-induced resistance are common in HNSCC patients. In the current work, we demonstrate that cisplatin treatment induces senescence in both p53 wild- type HN30 and p53 mutant HN12 head and neck cancer models. We also show that tumor cells can escape from senescence both in vitro and in vivo. We further established a cisplatin-resistant cell line from HN30 parental cell line that underwent brief senescence …

Laying Out A Space: Spectral Geographies, Fictions Of The Soul, Erin D. Yerby

Theses and Dissertations

Laying out a Space: Spectral Geographies, Fictions of the Soul, arises out of my artistic practice, and thoughts behind my current project and MFA exhibition, Spectral Geographies.

Linking the problem of the world ‘out there’ or external space, to inner experience through painting as both medium and practice, my work expresses what I call inner geographies, spaces where intimate immensities, folding inside and outside, find expression. I think of my paintings as beginning with this gesture of laying out a between-space where the intimacies of waking dreams and visions are opened by, and grow into, actual places, …

I'M Not Sure It Was A Hoopoe Or A Decanter, Ali Kaeini

Theses and Dissertations

“I’m not sure it was a Hoopoe or a Decanter” explores the experience of cultural displacement through the lens of an Iranian artist and immigrant. Drawing on ancient Iranian stories, Persian poetry, and cultural artifacts, my paintings and drawings reflect the uncertainty of the contemporary political and religious conditions in Iran, as well as the challenges faced by individuals forced to flee their homeland due to war, political instability, and social conditions. My work weaves references to ancient Iranian objects, Persian miniatures' structural forms and patterns, and Islamic architecture, using traditional and contemporary materials to create large, unconventional paintings. Through …

Old Joe, Ron Bland

Mighty Pen Project Anthology & Archive

In Vietnam, an old elephant wanders an abandoned plantation, striking wonder and sadness in a young Army helicopter gunner.

Articles, stories, and other compositions in this archive were written by participants in the Mighty Pen Project. The program, developed by author David L. Robbins, and in partnership with Virginia Commonwealth University and the Virginia War Memorial in Richmond, Virginia, offers veterans and their family members a customized twelve-week writing class, free of charge. The program encourages, supports, and assists participants in sharing their stories and experiences of military experience so both writer and audience may benefit.

Stage-Structured Predator-Prey Interactions In A Warming World: A Case Study In Riverine Rock Pools, Andrew T. Davidson

Theses and Dissertations

Warming can impact predator-prey interactions through a variety of mechanisms. For example, warming can both alter the rate at which predators consume prey and the rate prey develop through vulnerable life stages. Thus, the overall effect of warming on consumer-resource interactions will depend upon the strength and asymmetry of warming effects on predator and prey performance. Here, we describe the consequences of these asymmetrical responses to temperature by first developing a simple stage-structured modeling framework, then applying that framework to predation of American rock pool mosquito larvae, Aedes atropalpus, by several common predators in riverine rock pools. We then …

Interactions Between Hiv And Opioids On Antiretroviral Accumulation, The Blood Brain Barrier, And The Inflammatory Response In The Brain., Kara Rademeyer

Theses and Dissertations

The complex mechanisms related to HIV infection, neurodegeneration, and chronic neuroinflammation collectively describe neuroHIV (Hauser et al. 2007; Chang et al. 2014; Smith et al. 2014). Specifically, opioid abuse, poor penetration of antiretroviral (ARV) drugs, chronic inflammation and neuronal injury/degeneration are all implicated in neuroHIV (Fantuzzi et al. 2003; Letendre et al. 2004; Verani et al. 2005; Duncan and Sattentau 2011; Hong and Banks 2015; Simoes and Justino 2015; Olivier et al. 2018; Murphy et al. 2019; Osborne et al. 2020). For the first time, we demonstrate that morphine, fentanyl, and methadone in vivo alter the brain accumulation of ARVs, …

Climate, Physiology, And Distributions: The Role Of Thermal Physiology In Biological Invasions, Sean D. Powers

Theses and Dissertations

Climate is a primary factor influencing species range dynamics, particularly for ectotherms whose body temperature is closely tied to the surrounding conditions. While range shifts of ectotherms are attributed to climate warming, the mechanism driving these shifts are not well understood. Studies in macrophysiology demonstrate that the interaction of climate with thermal physiology plays a key role in determining large-scale spatial and temporal patterns for many temperature-sensitive physiological traits. This work has revealed a clear relationship between thermal tolerance breadth and range size. However, more recent analyses of this relationship demonstrate that thermal tolerance breadth only provides a partial explanation …

Probing The Porphyromonas Gingivalis Nitrosative Stress Response Network, Alexandra Galina

Theses and Dissertations

Objective: To persist in the oral cavity, the perio-pathogen Porphyromonas gingivalis must have an efficient nitrosative stress response mechanism. To better understand how P. gingivalis responds to nitrite (NO₂^-) we must determine what genes are involved in the nitrosative stress response network.

Methods: Through genomic screening, a list of genes was generated that were hypothesized to be part of the nitrosative stress response network and believed to be involved in nitrate/nitrite metabolism or redox state of the cells. Protein coding regions were replaced with the ermF sequence by allelic exchange mutagenesis. 15 mutants were created …

The Promis Women Education Program: Promoting Cervical Cancer Prevention Methods Among Muslim Women In Virginia, Asmaa M. Namoos

Theses and Dissertations

Abstract

Purpose: US Muslim women have suboptimal participation in cervical cancer screening and prevention services. This is due in part to a lack of access to culturally relevant education programs. To address this issue three aims were identified: Aim 1. Adapt existing evidence- based educational materials for a religious and culturally appropriate intervention; Aim 2. Test the preliminary efficacy of the intervention and Aim 3. Assess the feasibility and acceptability of the intervention and materials.

Methods: The ORBIT model for developing behavioral interventions guided intervention design, refinement (Phase I), and preliminary testing (Phase II). Focus groups and interviews were conducted …

Waiting To Exhale, Abigail H. Ogle

Theses and Dissertations

We breathe as a measure of time, it keeps us alive, and fabricates the pattern of our lives. We are punctuated by “snarls,” “glitches,” or moments of irregularity – of trying to catch one's breath, having it taken away, or gasping for it. It is the punctuation of sighs, huffs, sniffs, scoffs, screams, and deep intakes that appear as glitches in the breathing system.

In our daily rhythm of breathing, the presence of the glitch, defined as potentiality, can create space for something unexpected or new to arise. Using the wind from fans and approximately 1,260 square feet of silk, …

Illusion: Immersive Experience, Tessa S. Trowbridge

Theses and Dissertations

Can society learn from art, and if so, what type of knowledge can be gained from art? It is currently understood that art and design can help humans explore, discover, and understand philosophical and imaginative topics. There is a general agreement that art can create insight and awareness in ways that logical and rational statements cannot, and from these unique interactions, humans can see the world with a new perspective (Worth, n.d.). This thesis will investigate the claim that art can inspire human imagination and allow viewers to gain insight into a surreal reality using designed, physically immersive spaces.

Immersive …

Pwatem: An Anthology Of Literature And Art (2023)

Pwatem (Poictesme), 2006-

Pwatem is an anthology of literature and art from undergraduate students at Virginia Commonwealth University. Pwatem publishes poetry, prose and art of all kinds from talented undergraduate students of all majors.

Activity Levels Of Different Cell Types In An Epileptogenic Model Of Cortical Microgyria: A Cfos Investigation, Peter F. Lamothe

Theses and Dissertations

A large percentage of individuals with intractable epilepsies have an accompanying cortical malformation, the underlying cellular mechanisms of which are not fully understood. In an animal model for one such malformation, polymicrogyria, epileptogenesis occurs most easily in a region of tissue just adjacent to the microgyria termed the paramicrogyral region (PMR). Previous studies implicate somatostatin containing interneurons (SOM) as a potential contributor to this pathology, and show increased excitation of SOM in the PMR. We hypothesis that SOM are more active in the PMR when compared to SOM within the homologous region of the control cortex. In addition to this …

Formation Of Vasculogenic Mimicry: Role Of Melanoma Differentiation Associated Gene-9 In Human Melanoma, Jinkal Shah

Theses and Dissertations

Malignant melanoma (MM) is the most aggressive skin cancer and the most frequent skin disorder in Caucasians. MM is associated with aggressive and progressive disease states, leading to major cancer-related morbidity and mortality. Recent investigations identify an alternative pathway -vasculogenic mimicry (VM), which is considered a cancer hallmark that can independently facilitate tumor neovascularization by the formation of fluid-conducting and vascular endothelial cells. MM cells undergoing VM can dedifferentiate into numerous cellular phenotypes and acquire endothelial-like features, resulting in the formation of the de novo matrix-rich vascular-like networks, such as plasma and red blood cells. The co-generation of endothelial cells, …

Horizontal And Vertical Transmission Of Microbes Associated With Blow Flies Of Forensic Importance, Gabrielle Alisa Burton

Master of Science in Forensic Science Directed Research Projects

Entomological and microbiological evidence can be used in the estimation of postmortem intervals (PMI). This study observes the horizontal and vertical transmission of the microbial community associated with blow flies of forensic importance. Variable region four (V4) of the 16S ribosomal DNA (16S rDNA) was amplified and sequenced from various life stages of blow flies (n=72) using dual-index high throughput sequencing strategy on the MiSeq FGx platform. No significant difference in bacterial community structure was observed between blowfly adults with carrion access and blowfly adults with no carrion access. Dysgonomonas, unclassified Proteobacteria, Escherichia, Ignatzschia, Providencia, …

Bones As An Alternative Matrix For The Detection Of New Synthetic Opioids In Cold Cases: Introducing New Animal Models For Bone:Blood Correlation Studies, Kala N. Babb

Master of Science in Forensic Science Directed Research Projects

Introduction: The growing opioid epidemic in North America presents a call to action to develop new techniques for the identification and quantitation of novel synthetic opioids in uncommon matrices. In instances of extreme decomposition and/or significant scavenging, bone may be the only matrix available. In order to develop an extraction method for synthetic opioids in bone, bone samples were obtained from animal models. The animal model of choice is the rabbit due to their exhibition of spontaneous cortical bone remodeling, a process that is exhibited in humans but not in rats and mice.

Methods: An extraction method was developed to …

Investigation Into The Relationship Between Dna Content And Dna Yield Prediction And Presence Of A Multiple Contributor Cell Population In Trace Dna, Sarah Holton Roisch

Master of Science in Forensic Science Directed Research Projects

Due to advancements in DNA analysis technology and an increased ability to detect trace quantities of low template DNA, ‘touch’ or trace DNA has become a prevalent form of evidence for forensic investigations. Trace DNA is biological material left on a surface from skin cells after being touched or handled. The success rate of casework could be improved with new methods to non-destructively screen trace DNA to predict DNA yield and whether multiple cell populations are present in the evidence prior to STR analysis. Current methods of trace DNA analysis require quantifying the sample with qPCR followed by STR analysis, …

Optimization Of Microfluidic Device Methodology For Optical Trapping Epithelial Cells In A Mixture, Brittney N. Hackworth

Master of Science in Forensic Science Directed Research Projects

Evidence containing biological mixtures can make DNA interpretation more difficult due to ambiguous allele calls and artifacts that may be present within electropherograms. Separating out these cell mixtures can be advantageous to the analysts’ analysis methods for identifying STR profiles with higher accuracy. A potential cell separation method that could be implemented in the beginning of the forensic DNA workflow is optical trapping.

This research utilized a 5W ytterbium linearly polarized laser split into two optical traps, paired with a microfluidic device to isolate spermatozoa and vaginal epithelial cells from a 1:1 mixture of vaginal fluid and 1:20 diluted seminal …

Comparison Of Cell Counts To Dna Yield In Mock Biological Evidence Samples, Jacob Sewell

Master of Science in Forensic Science Directed Research Projects

The implementation of rapid DNA analysis systems is of interest to forensic DNA casework, as this technology exhibits a sample-to-profile time of approximately two hours with limited handling of samples. This decreases the likelihood of contamination, and can potentially reduce DNA backlogs; however, current rapid DNA analysis systems do not execute one crucial step of the forensic DNA analysis workflow: human DNA quantitation. This step is necessary for DNA profiles to be compliant with the FBI’s Quality Assurance Standard 9.4, allowing DNA profiles to be uploaded and searched within the CODIS database. Coupling traditional qPCR methods with rapid DNA analysis …

Evaluation Of Direct-To-Amplification Cell Lysis Techniques For Forensically Relevant Non-Sperm Cells, Rhea Arya

Master of Science in Forensic Science Directed Research Projects

While efforts have been made to reduce the pervasive backlog of sexual assault evidence collection kits, the actual laboratory process remains very time-consuming due to the requirement of a differential lysis step before DNA purification, as well as intricate mixture analysis towards the end of the DNA workflow. Previous work in the Dawson Green laboratory at VCU has developed alternative solutions for differential extractions with sexual assault samples using both an in-tube and microdevice assay. Prior work led to the identification of an alternative sperm lysis method – alkaline lysis using 1M NaOH. However, the current lysis method used for …

Unmet: The Undergraduate Experience Of Basic Need Insecurity At An Uncommon University, Michelle C. Bair, Rachelle V. Blackwell-Stegall, Sara E. Rafuse, Ra’Meer J. Roberts, Sara E. Weinstein

Doctor of Education Capstones

Virginia Commonwealth University’s (VCU) Dean of Students office submitted a Request for Assistance with needs related to food and housing insecurity and lack of basic funds for students. To address this request, a doctoral Capstone team conducted a problem and context analysis, literature review, student focus groups, and an internal survey of departments. The goal was to explore the undergraduate experience of unmet needs and to identify gaps and overlaps in basic needs support and services already provided at VCU. Findings suggest that students do not know the resources available to them through the Dean of Students office, that students …

Exploring The Relationship Between The Epigenome And Metabolism In Mycn-Amplified Neuroblastoma, Krista M. Dalton

Theses and Dissertations

High risk neuroblastoma (NB) is responsible for nearly 15% of all cancer related deaths in the pediatric population. The transcription factor MYCN is the most studied oncogene in NB, automatically conferring high-risk and has been found to be difficult to target. One way that MYCN amplification in NB functions to support tumorigenicity is through altered metabolism, satisfying the demand for rapid proliferation by increasing the nutrient flux through biosynthetic pathways. We performed an unbiased screen on select metabolic targeted therapy combinations and correlated sensitivity with over 20 subsets of cancer. We found that MYCN-amplified NB is hypersensitive to the …

Advancing Social Equity And Promoting Black Self-Determination: A Community Garden Opportunity Plan For Richmond, Va, Alessandro U. Ragazzi

Master of Urban and Regional Planning Capstone Projects

Urban agriculture provides a variety of economic, environmental, and social benefits. Recognizing these benefits, the City of Richmond administers a community garden program known as “Richmond Grows Gardens.” Through this program, underutilized city properties are permitted for use as community gardens. However, an emerging concern is the potential for community gardens to perpetuate underlying structures of social and racial inequality through displacement and social exclusion. Considering these concerns, this plan investigates the implementation of community gardens in Richmond and establishes a methodology for ranking existing and available community garden sites based on the social and racial demographics of their surrounding …

Developing Agrihoods: The Context For Petersburg, Brandon L. Archer

Master of Urban and Regional Planning Capstone Projects

Petersburg has an extensive history of navigating through challenging times. From its inception, African Americans have played an important role in the development of the city, and today that spirit is still present. As evidenced by an extensive network of community support and stakeholders, the Petersburg Youth Farm has matured into a resource for not just the residents of the neighborhood but of the city as a whole. By incorporating some innovative planning techniques and building a housing development truly dedicated to community needs, we can improve lived experiences in the city.

By adopting the recommendations set forth in this …

Trolley Line Trail Creative Placemaking Plan, Courtland Caldwell

Master of Urban and Regional Planning Capstone Projects

The Trolley Line Trail is a planned multi-use trail along the historic Richmond-to-Ashland Trolley corridor. The purpose of this plan is to identify potential creative placemaking locations and installations to highlight the trolley's unique past and present. This plan sets forth a vision for creative placemaking by gathering community feedback, analyzing properties along the Trolley Line Trail, and making recommendations for placemaking and public art. Ultimately, the recommendations laid out in this plan aim to preserve and acknowledge the community's unique identity while creating a safe, accessible experience for all users.

Windmill Ridge Park: A Sustainable Park Design, Sean Benson

Master of Urban and Regional Planning Capstone Projects

This plan proposes designing and developing a new park in Chesterfield County that will promote sustainability in its design and implementation, as well as provide a stepping stone to promote these practices throughout Chesterfield County parks. This plan achieves this by providing research, concepts, and recommendations centered on the three Es of sustainability: environmental quality, social equity, and economic efficiency.

Clinical Inquiry Question Development Checklist, Roy E. Brown

VCU Libraries Faculty and Staff Publications

The Clinical Inquiry Question Development Checklist is a handout used in instructional sessions with nurses about the clinical inquiry process. This process includes evidence-based practice, performance improvement, and research. The revised handout contains information related to the performance improvement process and various other aspects of the clinical inquiry process.

The Clinical Inquiry Process Diagram Question Development Checklist, Roy E. Brown

VCU Libraries Faculty and Staff Publications

The Clinical Inquiry Question Development Checklist is a handout used in instructional sessions with nurses about the clinical inquiry process. This process includes evidence-based practice, performance improvement, and research. The revised handout closely aligns with the 4^th version of the Clinical Inquiry Process Diagram and contains information related to the performance improvement process and various other aspects of the clinical inquiry process.

Understanding The Implications Of Work Based Learning For Students Pk-12 School Systems Institutions Of Higher Education And Hosting Organizations, David Naff, Amy Corning, Meleah Ellison, Albion Sumrell, Zehra Sahin Ilkorkor, Jennifer Murphy, Ciana Cross

MERC Publications

This literature review by the Metropolitan Educational Research Consortium (MERC) and Institute for Collaborative Research and Evaluation (ICRE), in partnership with the Virginia Talent + Opportunity Partnership (V-TOP) and State Council of Higher Education for Virginia (SCHEV) explores work-based learning and its implications for PK-12 institutions, institutions of higher education, and hosting organizations (e.g. employers). The report also provides background information about the foundations of work-based learning and concludes with a series of recommendations for practice, policy, and future research related to work-based learning. There is also an accompanying podcast episode where report authors discuss the key takeaways with …

Molecular Target And Circuitry Underlying The Preclinical Effects Of Psychedelics In Models Of Opioid Use Disorder, Alaina M. Jaster

Theses and Dissertations

Opioid use disorder (OUD), like other substance use disorders (SUD), is characterized by maladaptive behavioral allocation away from other rewarding aspects of life following prolonged use, as well as large rates of relapse. While pharmacotherapies exist for OUD, they tend to only target the opioid receptors rather than the underlying mechanisms behind the maladaptive behaviors. Serotonin (5-hydroxytryptamine; 5-HT) is involved in modulation of cravings and regulation of motivational reward-related behaviors, thought to be due to a top-down processing method of external and internal stimuli. Serotonin 2A receptors (5-HT_2AR) are widespread throughout the brain and most densely populate layer …