Digital Commons Network^™

The Effects Of Sdf-1Α Treatment On The Migration Of Neural Stem/Progenitor Cells After Traumatic Brain Injury, Corey Evans

Theses and Dissertations

Traumatic Brain Injury (TBI) is one of the leading causes of death and disability among young adults and has been a significant field in medical research over the past decades. Intensive studies focusing on how to repair tissue damage resulting from head injuries have discovered that the central nervous system (CNS) retains a regenerative capacity throughout life due to the persistent presence of neural stem/progenitor cells (NS/NPCs) in the neurogenic regions. In the normal brain, cells generated in the subventricular zone (SVZ) migrate along the rostral migratory stream (RMS) to the olfactory bulb and cells in the subgranular zone (SGZ) …

Objectionable Team Nicknames: Determining The Likelihood Of Selling The Issue Of Banning Them In Virginia High Schools, Pamela Taylor

Theses and Dissertations

This study explored the personal and situational factors that contribute to a high school principal's willingness to sell the issue of objectionable team nicknames to their school division administration for the purposes of banning them. Based on the literature review, nine hypotheses were developed regarding the factors that influence the issue-selling process in a centralized, hierarchical organization. The issue-selling model utilized in this study suggested that organizational support, top management openness, organizational norms, probability of success, and image risk would be determinants of willingness to sell the issue (Mullen, 2005). This study utilized a mixed-method research design. Personal interviews were …

Wilms’ Tumor-1 (Wt1) Protein Expression In Glioma Cells Actuates Cellular Invasiveness- Identifying Its Target Genes, Archana Chidambaram

Theses and Dissertations

Previous studies in our laboratory demonstrated the expression of WT1 in a significant number of glioma cells and established its role in promoting tumor cell proliferation. Here, we noted the effect(s) of manipulating WT1 levels on the expression levels of genes that were previously shown to be regulated by WT1. We found no correlation between the expression levels of WT1 and PDGF-A, Snai1 and E-cadherin and a consistent inverse correlation between WT1 and IGF-1R expression in U251-MG cells. To ascertain whether the increased IGF-1R levels resulting from WT1 silencing could account for decreased cellular proliferation, we utilized siRNA mediated knockdown …

Kinase Pathways Underlying Muscarinic Activation Of Colonic Longitudinal Muscle, Charles Dudley Anderson Jr.

Theses and Dissertations

The longitudinal muscle layer in gut is the functional opponent to the circular muscle layer during the peristalsis reflex. Differences in innervation of the layers allow for the contraction of one layer that corresponds with the simultaneous relaxation of the other, enabling the passage of gut contents in a controlled fashion. Differences in development have given the cells of the two layers differences in receptor populations, membrane lipid handling, and calcium handling profiles/behaviors. The kinase signaling differences between the two layers is not as well characterized. Upon activation of cells from the circular muscle layer, it is known that Rho …

Commonwealth Times 2011-04-21

Commonwealth Times, 1969-

No abstract provided.

Characterization Of A Blast Wave Device And Blast Wave Induced Traumatic Brain Injury In A Rat Model By Magnetic Resonance Imaging And Spectroscopy, Frank Corwin

Theses and Dissertations

Blast wave induced traumatic brain injury (bTBI) is a modality of injury that has come into prominence at the current time due to the large number of military and civilian personnel who have experienced the localized shock wave produced by explosive devices. The shock wave will travel concentrically outward from the explosive center, being absorbed and transmitted thru soft objects, such as tissue, and reflecting off stationary obstructions. Transmission and absorption in tissues can result in a number of physiological measureable injuries, the most common of which being what is frequently called “blast lung”. Blast lung involves the spalling effect …

Commonwealth Times 2011-04-18

Commonwealth Times, 1969-

No abstract provided.

Dialogue In The Galleries: Developing A Tour About Contemporary Art For The Virginia Museum Of Fine Arts, Elizabeth Reilly-Brown

Theses and Dissertations

This museum thesis project considers the challenges involved in developing engaging museum tours. The purpose of this project was to develop a fifty-minute, guided gallery tour that uses inquiry-based instruction to engage participants in dialogue and critical thinking about artworks. The tour was designed specifically for the Virginia Museum of Fine Arts (VMFA) in Richmond, Virginia, using artworks selected from the museum’s twenty-first-century art collection that relate to the theme hybridity. This project contributes to the museum studies field by exemplifying how gallery tours can stimulate active learning, encourage visitors to find meaning in artworks, and form their own conclusions …

Layers Of Meaning, Joan Wiberg

Theses and Dissertations

My artwork reflects my search for my own artistic voice. Life demands examination from me at inconvenient times, but with the intention of growing as a person I proceed willingly, and as an artist, equipped with a paintbrush. My search has led me to discover new materials and techniques and my structured self-reflection has uncovered a new source of inspiration. I now combine the physical layering of art materials with layers of personal meaning as well. My work has an autobiographical theme which I have interpreted through the filter of my sense of humor. Working so intimately with my own …

Commonwealth Times 2011-04-07

Commonwealth Times, 1969-

No abstract provided.

Differential Regulation Of Mlc20 Phosphorylation In Tonic And Phasic Smooth Muscles Of The Stomach, Othman Al-Shboul

Theses and Dissertations

Gastrointestinal (GI) smooth muscle possesses distinct regional and functional properties that distinguish it from other types of visceral and vascular smooth muscle. On the basis of electrical properties and contractile phenotype, GI smooth muscles have been classified into phasic (non-sphinteric) and tonic (sphinteric) smooth muscles. The biochemical basis of phasic and tonic phenotypes of smooth muscle is not clear and is the major question of inquiry of the present study. Phosphorylation of Ser19 on the 20 kDa myosin light chain (MLC) is essential for acto-myosin interaction and contraction in both phasic and tonic muscles. The levels of MLC20 phosphorylation are …

Commonwealth Times 2011-05-02

Commonwealth Times, 1969-

No abstract provided.

Iain Baxter& And N.E. Thing Co.: A Study In Pop-Inflected Conceptual Art, Dennis Durham

Theses and Dissertations

The Canadian artist IAIN BAXTER&, known before 2005 as Iain Baxter, created an innovative Conceptual Art practice in the mid-60s that continues to make important contributions even today. He has maintained a strong collaborative element in his art, as witnessed by his role in the short-lived group IT (1965) and N.E. THING CO. (1966-1978)--an actual incorporated company consisting of BAXTER& and his first wife, Ingrid Baxter as chief officers--and by the addition of an ampersand to his legal name in 2005 to signify the open-ended quality of his work that relies on viewers‘ contributions to help determine its meaning. This …

Commonwealth Times 2011-03-31

Commonwealth Times, 1969-

No abstract provided.

Commonwealth Times 2011-03-28

Commonwealth Times, 1969-

No abstract provided.

Journey, Michael Mcgaugh

Theses and Dissertations

My art explores the traditional elements of line, value, and texture. I use pencil and charcoal to create my drawings, and use the computer to alter and enhance my photographs. I also explore the relationships created through the combination of traditional and digital processes. Through the process of drawing, scanning, and layering images on the computer, I reveal details created by the contrast of these media. My goal is to successfully combine and balance traditional art practices with digital technology.

Characterization And Genetic Analysis Of The Cellulolytic Microorganism Thermobifida Fusca, Yu Deng

Theses and Dissertations

Currently, one of the hurdles hindering efficient production of cellulosic biofuel is the recalcitrant nature of cellulose to hydrolysis. A wide variety of cellulase enzymes are found natively in microorganisms that can potentially be used to effectively hydrolyze cellulose to fermentable sugars. Thermobifida fusca is a high G-C content, thermophilic, gram-positive soil actinobacterium with high cellulolytic activity. The phenomenological and mechanistic parameters affecting cellulase activity were studied in T. fusca and two mechanisms have been found: 1) transcriptions of cellulase-related genes were not closely associated with measured differences in cellulase activity and 2) cellular energetics (intracellular ATP) correlated more closely …

This Time Tomorrow And Other Painted Stories, Faylinda Kodis

Theses and Dissertations

My visual vocabulary is drawn from the language of fairytales and dreams and includes animals, birds, tambourines, scrolled railings, clumps of flowers, clocks, bubbles, and people. I include symbols that resonate for me or, through its shape, solve a formal compositional problem. The images are pieced together in a collage-like manner and juxtaposed without regard to real world concerns of place, size, and scale. The resulting stories have a surreal appearance. My goal is to create drawings and paintings that reveal a personal, expressive reality.

Commonwealth Times 2011-03-24

Commonwealth Times, 1969-

No abstract provided.

Commonwealth Times 2011-03-21

Commonwealth Times, 1969-

No abstract provided.

Commonwealth Times 2011-03-10

Commonwealth Times, 1969-

No abstract provided.

Commonwealth Times 2011-03-07

Commonwealth Times, 1969-

No abstract provided.

The Epidemiology Of Human Rabies Postexposure Prophylaxis In Virginia, 2002 And 2003, Marilyn Goss Haskell

Theses and Dissertations

Objective: To describe a sample that received human rabies postexposure prophylaxis (PEP) in Virginia as a result of animal exposures in 2002 and 2003 and to determine the extent to which PEP decisions were appropriate.Methods: PEP surveillance data were requested from 35 Virginia health districts within 5 regions. Retrospective chart review was used to gather demographic, exposure and source animal data from patient records and animal exposure reports. Descriptive statistics are presented. True exposures and appropriateness of PEP were defined using the 2004 Virginia Rabies Control Guidelines and the Recommendations of the 1999 Advisory Committee on Immunization Practices. The 2003 …

Identification And Characterization Of Low Ph-Triggered Conformational Changes In The Herpes Simplex Virus Glycoprotein B, Stephen Dollery

Theses and Dissertations

Herpesviruses can enter host cells by pH-dependent endocytic pathways in a cell-specific manner. The role of pH in herpesvirus endocytosis is unclear. Herpes simplex virus (HSV) is a paradigm for virus membrane fusion via a complex of glycoproteins. HSV glycoproteins B, D and the heterodimer H-L are necessary and sufficient for membrane fusion. This work analyzes the structure and function of HSV glycoproteins B, D, and H-L at neutral pH, and at the physiological low-pH encountered during endocytic entry. It is demonstrated that mildly acidic low pH triggers specific conformational changes in HSV gB at a pH of 5.7 to …

Fused, Lauren Cifranic

Theses and Dissertations

I use encaustic, wire, and oil paint on wooden boxes and panels. I am interested in the process of layering media. These forms evolve as previous marks are concealed, added to, and concealed again while intuitively creating forms. With this body of work I explore confinement and freedom. Therefore, some of the shapes and forms seem contained while others appear to break away from their boundaries.

Ink (2011-02)

Ink, 2008-

The student-run features magazine covering the fashion, art, music and culture worlds at VCU and in Richmond. Published annually in print and updated regularly online.

Corticotrophin-Releasing Hormone Type 1 Receptor Gene (Crhr1) Variants Predict Posttraumatic Stress Disorder Onset And Course In Pediatric Injury Patients, Ananda B. Amstadler, Nicole R. Nugent, Bao-Zhu Yang, Alisa Miller, Richie Siburian, Priya Moorjani, Stephen Haddad, Aditi Basu, Jesen Fagerness, Glenn Saxe, Jordan W. Smoller, Karestan C. Koenen

Psychiatry Publications

Posttraumatic stress disorder (PTSD) is a common and disabling anxiety disorder that may occur in the aftermath of exposure to potentially traumatic life events. PTSD is moderately heritable, but few specific molecular variants accounting for this heritability have been identified. Genes regulating the hypothalamic-pituitary-adrenal (HPA) axis, such as corticotrophin-releasing hormone type 1 receptor gene (CRHR1), have been implicated in traumatic-stress related phenotypes but have yet to be studied in relation to PTSD. The present study sought to examine the relation between 9 single nucleotide polymorphisms (SNPs) in the CRHR1 gene and posttraumatic stress symptoms in a prospective study of pediatric …

Acsl6 Is Associated With The Number Of Cigarettes Smoked And Its Expression Is Altered By Chronic Nicotine Exposure, Jingchun Chen, Darlene H. Brunzell, Kia J. Jackson, Andrew Van Der Vaart, Jennie Z. Ma, Thomas J. Payne, Richard Sherva, Lindsay A. Farrer, Pablo Gejman, Douglas F. Levinson, Peter Holmans, Steven H. Aggen, Imad Damaj, Po-Hsiu Kuo, Bradley T. Webb, Raymond Anton, Henry R. Kranzler, Joel Gelemter, Minf D. Li, Kenneth S. Kendler, Xiangning Chen

Psychiatry Publications

Individuals with schizophrenia tend to be heavy smokers and are at high risk for tobacco dependence. However, the nature of the comorbidity is not entirely clear. We previously reported evidence for association of schizophrenia with SNPs and SNP haplotypes in a region of chromosome 5q containing the SPEC2, PDZ-GEF2 and ACSL6 genes. In this current study, analysis of the control subjects of the Molecular Genetics of Schizophrenia (MGS) sample showed similar pattern of association with number of cigarettes smoked per day (numCIG) for the same region. To further test if this locus is associated with tobacco smoking as measured …

Genomic Analysis Of Individual Differences In Ethanol Drinking: Evidence For Non-Genetic Factors In C57bl/6 Mice, Jennifer T. Wolstenholme, Jon A. Warner, Maria I. Capparuccini, Kellie J. Archer, Keith L. Shelton, Michael F. Miles

Pharmacology and Toxicology Publications

Genetic analysis of factors affecting risk to develop excessive ethanol drinking has been extensively studied in humans and animal models for over 20 years. However, little progress has been made in determining molecular mechanisms underlying environmental or non-genetic events contributing to variation in ethanol drinking. Here, we identify persistent and substantial variation in ethanol drinking behavior within an inbred mouse strain and utilize this model to identify gene networks influencing such “non-genetic” variation in ethanol intake. C57BL/6NCrl mice showed persistent inter-individual variation of ethanol intake in a two-bottle choice paradigm over a three-week period, ranging from less than 1 g/kg …

Genetic Analysis Of The Neurosteroid Deoxycorticosterone And Its Relation To Alcohol Phenotypes: Identification Of Qtls And Downstream Gene Regulation, Patrizia Porcu, Todd K. O'Buckley, Soomin C. Song, Jo Lynne Harenza, Lu Lu, Xusheng Wang, Michael F. Miles, A. Leslie Morrow

Neurology Publications

Background

Deoxycorticosterone (DOC) is an endogenous neurosteroid found in brain and serum, precursor of the GABAergic neuroactive steroid (3α,5α)-3,21-dihydroxypregnan-20-one (tetrahydrodeoxycorticosterone, THDOC) and the glucocorticoid corticosterone. These steroids are elevated following stress or ethanol administration, contribute to ethanol sensitivity, and their elevation is blunted in ethanol dependence.

Methodology/Principal Findings

To systematically define the genetic basis, regulation, and behavioral significance of DOC levels in plasma and cerebral cortex we examined such levels across 47 young adult males from C57BL/6J (B6)×DBA/2J (D2) (BXD) mouse strains for quantitative trait loci (QTL) and bioinformatics analyses of behavior and gene regulation. Mice were injected with saline …