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Genetic Engineering In Agriculture Curricula For Grades 9-12, Tyson Sorensen, Olivia Horning, Kelsey Hall, David Francis, Joshua Dallin

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This is a curriculum package that was developed for teachers to be able to integrate genetic engineering and biotechnology concepts, skills, and career applications into their classrooms. This package was developed as part of the LEARN workshop entitled "Genetic Engineering: Workshop for Teachers." This curriculum package includes five full units of instructions with lesson plans, presentation resources, and other resources for teachers. This package is intended for students in grades 9-12.

Each unit is complete with the corresponding slides found in the main Genetic Engineering PowerPoint. Teachers have the liberty to cover the material on an as-needed bases based on …

Pathogenesis Of Rift Valley Fever Virus Aerosol Infection In Stat2 Knockout Hamsters, Brady T. Hickerson, Jonna B. Westover, Arnaud J. Van Wettere, Johanna D. Rigas, Jinxin Miao, Bettina L. Conrad, Neil E. Motter, Zhongde Wang, Brian B. Gowen

Animal, Dairy, and Veterinary Science Faculty Publications

Rift Valley fever virus (RVFV) is an emerging pathogen capable of causing severe disease in livestock and humans and can be transmitted by multiple routes including aerosol exposure. Several animal models have been developed to gain insight into the pathogenesis associated with aerosolized RVFV infection, but work with these models is restricted to high containment biosafety level (BSL) laboratories limiting their use for antiviral and vaccine development studies. Here, we report on a new RVFV inhalation infection model in STAT2 KO hamsters exposed to aerosolized MP-12 vaccine virus by nose-only inhalation that enables a more accurate delivery and measurement of …

Grandma's Shaking Ghost, Amber Utterback

USU Student Folklore Fieldwork

My grandparents moved a mobile home onto our property when my dad got sick. They now permanently live on the bottom of our hill. My cousins, my brother and I would spend the night almost every weekend in the summer. My grandparents have a coveted guestroom that we would all fight over because one of my cousins snored and we didn’t want to share the living room with her. One night, I was the lucky one so I got to sleep in the room by myself. It was at the end of the house and the twin bed was set …

Meow Meow, Amber Utterback

USU Student Folklore Fieldwork

A couple of years ago, I was getting home late from a church activity. We live on a farm in the middle of nowhere, our barn is across the driveway from our house, far enough away that the light from the front porch can’t reach the entrance to the barn. We never shut the large barn door--this door is big enough for tractors and stuff to pull through and at night it is pitch black in there. We always enter in through the garage so the garage door is always open too, but my parents never remember to turn the …

Manure Application Timing Drives Energy Absorption For Snowmelt On An Agricultural Soil, Melanie N. Stock, Francisco J. Arriaga, Peter A. Vadas, Krishnapuram G. Karthikeyan

Plants, Soils, and Climate Faculty Publications

Reducing agricultural runoff year-round is important, in particular during snowmelt events on landscapes that receive wintertime applications of manure. To help inform manure guidelines, process-level data are needed that link management scenarios with the complexity of snowmelt, hence runoff. Albedo and radiative energy fluxes are strong drivers of thaw, but applying these mechanistic measurements across multiple, plot-scale management treatments over time presents a logistical challenge. The objective of this study was to first develop a practical field approach to estimate winter albedo in plot-scale field research with multiple management scenarios. The second objective was to quantify the radiative drivers of …

Does Experience With Sagebrush In Utero And Early In Life Influence Use Of Sagebrush By Sheep?, Juan J. Villalba, Fred Provenza, Ashley Longmore

Poisonous Plant Research (PPR)

Learning from mother begins early in the developmental process and can have lifelong effects when it comes to foraging behavior. Pregnancy is not just an incubation period but a starting point for animal well-being and disease later in life. A better understanding of the effects that early exposure to unpalatable feeds impinges on their use later in life may help create management plans that utilize grazing animals to their full potential as landscape manipulators.

Thus, the objective of this research was to explore how experience in utero and early in life with sagebrush (Artemesia tridentata spp. tridentata) -a …

Use Of Auto-Germ To Model Germination Timing In The Sagebrush-Steppe, William C. Richardson, Dallin R. Whitaker, Kyler P. Sant, Nicholas S. Barney, Ryan S. Call, Bruce A. Roundy, Zachary T. Aanderud, Matthew D. Madsen

Articles

Germination timing has a strong influence on direct seeding efforts, and therefore is a closely tracked demographic stage in a wide variety of wildland and agricultural settings. Predictive seed germination models, based on soil moisture and temperature data in the seed zone are an efficient method of estimating germination timing. We utilized Visual Basic for Applications (VBA) to create Auto‐Germ, which is an Excel workbook that allows a user to estimate field germination timing based on wet‐thermal accumulation models and field temperature and soil moisture data. To demonstrate the capabilities of Auto‐Germ, we calculated various germination indices and modeled germination …

Crystallographic And Computational Characterization Of Methyl Tetrel Bonding In S-Adenosylmethionine-Dependent Methyltransferases, Raymond C. Trievel, Steve Scheiner

Chemistry and Biochemistry Faculty Publications

Tetrel bonds represent a category of non-bonding interaction wherein an electronegative atom donates a lone pair of electrons into the sigma antibonding orbital of an atom in the carbon group of the periodic table. Prior computational studies have implicated tetrel bonding in the stabilization of a preliminary state that precedes the transition state in S_N2 reactions, including methyl transfer. Notably, the angles between the tetrel bond donor and acceptor atoms coincide with the prerequisite geometry for the S_N2 reaction. Prompted by these findings, we surveyed crystal structures of methyltransferases in the Protein Data Bank and discovered …

Habitat Segregation Between Brown Bears And Gray Wolves In A Human-Dominated Landscape, Cyril Milleret, Andrés Ordiz, Guillaume Chapron, Harry Peter Andreassen, Jonas Kindberg, Johan Månsson, Aimee Tallian, Petter Wabakken, Camilla Wikenros, Barbara Zimmermann, Jon E. Swenson, Håkan Sand

Ecology Center Publications

Identifying how sympatric species belonging to the same guild coexist is a major question of community ecology and conservation. Habitat segregation between two species might help reduce the effects of interspecific competition and apex predators are of special interest in this context, because their interactions can have consequences for lower trophic levels. However, habitat segregation between sympatric large carnivores has seldom been studied. Based on monitoring of 53 brown bears (Ursus arctos) and seven sympatric adult gray wolves (Canis lupus) equipped with GPS collars in Sweden, we analyzed the degree of interspecific segregation in habitat selection …

Winds Of Change – Predicting Water-Based Recreationists' Support And Opposition For Offshore Wind Energy Development In The Great Lakes, Michael D. Ferguson, Samantha L. Powers, Nate E. Trauntvein, Jeffrey B. Jacquet, Alan R. Graefe, Andrew J. Mowen

Kinesiology and Health Science Faculty Publications

This study examined the factors influencing water-based recreationists' perceptions of support and opposition towards off-shore wind energy development (OWD) on Lake Erie. Much of the proposed or future Lake Erie OWD infrastructure may either be within or adjacent to public lands, waters, and protected areas, raising concerns about the potential environmental and social impacts upon recreation stakeholders. The limited body of OWD research within the United States has suggested there are numerous factors that may influence overall perceptions of support and opposition such as political orientation and beliefs in climate change. Moreover, recent research has proposed that the perceived recreation …

Wild Bees Of Grand Staircase-Escalante National Monument: Richness, Abundance, And Spatio-Temporal Beta-Diversity, Olivia Messinger Carril, Terry Griswold, James Haefner, Joseph S. Wilson

All PIRU Publications

Interest in bees has grown dramatically in recent years in light of several studies that have reported widespread declines in bees and other pollinators. Investigating declines in wild bees can be difficult, however, due to the lack of faunal surveys that provide baseline data of bee richness and diversity. Protected lands such as national monuments and national parks can provide unique opportunities to learn about and monitor bee populations dynamics in a natural setting because the opportunity for large-scale changes to the landscape are reduced compared to unprotected lands. Here we report on a 4-year study of bees in Grand …

Developmental Parameters Of A Southern Mountain Pine Beetle (Coleoptera: Curculionidae) Population Reveal Potential Source Of Latitudinal Differences In Generation Time, Anne E. Mcmanis, James A. Powell, Barbara J. Bentz

Mathematics and Statistics Faculty Publications

Mountain pine beetle (Dendroctonus ponderosae, Hopkins) is a major disturbance agent in pine ecosystems of western North America. Adaptation to local climates has resulted in primarily univoltine generation time across a thermally diverse latitudinal gradient. We hypothesized that voltinism patterns have been shaped by selection for slower developmental rates in southern populations inhabiting warmer climates. To investigate traits responsible for latitudinal differences we measured lifestage-specific development of southern mountain pine beetle eggs, larvae and pupae across a range of temperatures. Developmental rate curves were fit using maximum posterior likelihood estimation with a Bayesian prior to improve fit stability. …

Kinetic Characterization Of The Shigella Type Three Secretion System Atpase Spa47 Using Α-32p Atp, Heather B. Case, Nicholas E. Dickenson

Chemistry and Biochemistry Faculty Publications

ATPases represent a diverse class of enzymes that utilize ATP hydrolysis to support critical biological functions such as driving ion pumps, providing mechanical work, unfolding/folding proteins, and supporting otherwise thermodynamically unfavorable chemical reactions. We have recently shown that the Shigella protein Spa47 is an ATPase that supports protein secretion through its specialized type three secretion apparatus (T3SA), supporting infection of human host cells. Characterizing ATPases, such as Spa47, requires a means to accurately determine enzyme activity (ATP hydrolysis) as a function of time, reaction conditions, and potential cofactors, regulators, inhibitors, etc. Here, we describe a detailed protocol for characterizing …

Sediment Dynamics In The Bear River-Mud Lake-Bear Lake System, Patrick Belmont, Mitchell Donovan, Janice Brahney, Lindsay Capito, Zach Burgert

Watershed Sciences Faculty Publications

The overarching goal of this project was to compile and analyze a variety of existing datasets, and generate several new datasets, to advance our understanding of how the Bear River Mud Lake-Bear Lake system functions, how it has, or is expected to change, identify which components are degraded or vulnerable to degradation, and determine if/where critical data and/or knowledge gaps exist. We conducted a series of analyses to evaluate changes in hydrology and suspended sediment, collected sediment cores from nine locations in Mud Lake to evaluate how sedimentation rates, sediment sources and water quality have changed over time, and utilized …

In Situ Real-Time Quantification Of Microbial Communities: Applications To Cold And Dry Volcanic Habitats, Linda S. Powers, Heather D. Smith, Aminata P. Kilungo, Walther R. Ellis Jr., Christopher P. Mckay, Rosalba Bonaccorsi, Janet W. Roveda

Biological Engineering Faculty Publications

We report field tests of an instrument using multi-wavelength excitation and detection of fluorescence capable of detection and discrimination of viable cells, non-viable cells (not metabolically active but not decomposed), and spores in extreme arid environments where low microbial abundances are expected. These new results are presented for cold and dry volcanic habitats worldwide, e.g., the arid core of the Atacama Desert, Mt. Kilimanjaro glacier and Kibo area, Pali Aike caldera, and the western US volcanic and desert soils in Utah, Idaho, Nevada, and California. Our results are comparable to previous studies reported in the literature for the same environments. …

South American Leaf-Cutter Bees (Genus Megachile) Of The Subgenera Rhyssomegachile And Zonomegachile, With Two New Subgenera (Hymenoptera: Megachilidae), Víctor H. González, Terry Griswold, Michael S. Engel

All PIRU Publications

Leaf-cutter bees (genus Megachile Latreille) are among the most common and diverse group of bees. However, the identity and taxonomic placement of many species are problematic and species identification is often difficult. Some species are known only from a single specimen or from one of the sexes, and identification keys are not available for many groups. We address these taxonomic issues for the subgenera Rhyssomegachile Mitchell and Zonomegachile Mitchell, two poorly known South American lineages of leaf-cutter bees. We provide comparative diagnoses, redescriptions, illustrated identification keys, new geographical records, and designate needed neotypes for Megachile cara Mitchell, M. gigas Schrottky, …

Environmental Water Transactions In The Colorado River Basin: A Closer Look, Leon Szeptycki, David Pilz, Rachel O'Connor, Beatrice Gordon

All In-stream Flows Material

Over the last 30 years, the voluntary transfer of water and water rights for environmental uses has become a recognized strategy for restoring streamflow in the Western United States.¹ Historically, taking water out of a stream was a legally required element of an appropriative water right. Water rights holders who left all or a portion of their right instream to enhance fish populations, riparian habitat or recreation, risked forfeiture or diminishment of their water right. Beginning in the 1980’s, state laws began to recognize both the appropriation of new water rights for instream use and to allow the transfer …

Riparian Research And Management: Past, Present, Future: Volume 1, Steven W. Carothers, Hisham El Waer, Helen C. Fairley, Deborah M. Finch, Suzanne C. Fouty, Jonathan M. Friedman, Jennifer K. Frey, Stanley V. Gregory, Robert H. Hamre, Annie Henry, Osvel Hinojosa-Huerta, Elaine E. Johnson, R. Roy Johnson, Kenneth J. Kingsley, Mary Anne Mcleod, Eric Mellink, Katie Merewether, Duncan T. Patten, John S. Richardson, Anne Sands, Michael L. Scott, Bo Shelby, Anna A. Sher, D. Max Smith, John T. Stanley, Frederick J. Swanson, Raymond M. Turner, Robert H. Webb, William E. Werner

Watershed Sciences Faculty Publications

Fifty years ago, riparian habitats were not recognized for their extensive and critical contributions to wildlife and the ecosystem function of watersheds. This changed as riparian values were identified and documented, and the science of riparian ecology developed steadily. Papers in this volume range from the more mesic northwestern United States to the arid Southwest and Mexico. More than two dozen authors—most with decades of experience—review the origins of riparian science in the western United States, document what is currently known about riparian ecosystems, and project future needs. Topics are widespread and include: interactions with fire, climate change, and declining …

Genetic Variants In The Sirt6 Transcriptional Regulatory Region Affect Gene Activity And Carcass Quality Traits In Indigenous Chinese Beef Cattle (Bos Taurus), Lin-Sheng Gui, Sayed Haidar Abbas Raza, Matthew Garcia, Yong-Gang Sun, Irfan Ullah, Yin-Cang Han

Animal, Dairy, and Veterinary Science Faculty Publications

Background: The aim of this study was to analyze potential influences of polymorphisms within the regulatory region of the bovine SIRT6 gene on carcass quality traits. Expression analyses suggested that SIRT6 gene is predominately expressed in kidney, compared with other tissues. In 535 indigenous Chinese beef cattle, two novel single nucleotide polymorphisms (SNPs) were identified within the promoter region of the SIRT6 gene.

Results: Association analysis indicated that G allele of the c.-1100 A > G had a positive effect on fat deposition, and the Hap4/4 diplotype had more favourable results than other dipoltypes with respect to the evaluation of carcass …

Dependence Of Nmr Chemical Shifts Upon Ch Bond Lengths Of A Methyl Group Involved In A Tetrel Bond, Steve Scheiner

Chemistry and Biochemistry Faculty Publications

Four different Lewis acids that might participate in a tetrel bond with a nucleophile (SEt₂Me⁺, NMe₄⁺, SMe₂, NMe₃) are examined. The NMR chemical shifts of the methyl C and H atoms are calculated as the CH bond lengths are systematically stretched and contracted, in the absence of a base. The C shielding diminishes by roughly 2 ppm for a stretch of 0.01 Å, while that of H drops by only 0.3 ppm. The deshieldings caused purely by the bond length changes are far too small to account for the …

The Utah Statesman, October 30, 2018, Utah State University

The Utah Statesman

Weekly student newspaper of Utah State University in Logan.

Forecast Or Fall: Prediction's Importance To Postural Control, Chris J. Dakin, David A.E. Bolton

Kinesiology and Health Science Faculty Publications

To interact successfully with an uncertain environment, organisms must be able to respond to both unanticipated and anticipated events. For unanticipated events, organisms have evolved stereotyped motor behaviors mapped to the statistical regularities of the environment, which can be trigged by specific sensory stimuli. These 'reflexive' responses are more or less hardwired to prevent falls and represent, maybe, the best available solution to maintaining posture given limited available time and information. With the gift of foresight, however, motor behaviors can be tuned or prepared in advance, improving the ability of the organism to compensate for, and interact with, the changing …

Complexity Is Complicated And So Too Is Comparing Complexity Metrics—A Response To Mikula Et Al. (2018), William D. Pearse, Ignacio Morales-Castilla, Logan S. James, Maxwell Farrell, Frédéric Boivin, T. Jonathan Davies

Ecology Center Publications

In a recent publication (Pearse et al. 2018b), we explored the macroevolution and macroecology of passerine song using a large citizen science database of bird songs and powerful machine learning tools. Mikula et al. (2018) examine a small subset (

Hidden Cost Of Disease In A Free-Ranging Ungulate: Brucellosis Reduces Mid-Winter Pregnancy In Elk, Gavin C. Cotterill, Paul C. Cross, Arthur D. Middleton, Jared D. Rogerson, Brandon M. Scurlock, Johan T. Du Toit

Wildland Resources Faculty Publications

Demonstrating disease impacts on the vital rates of free‐ranging mammalian hosts typically requires intensive, long‐term study. Evidence for chronic pathogens affecting reproduction but not survival is rare, but has the potential for wide‐ranging effects. Accurately quantifying disease‐associated reductions in fecundity is important for advancing theory, generating accurate predictive models, and achieving effective management. We investigated the impacts of brucellosis (Brucella abortus) on elk (Cervus canadensis) productivity using serological data from over 6,000 captures since 1990 in the Greater Yellowstone Ecosystem, USA. Over 1,000 of these records included known age and pregnancy status. Using Bayesian multilevel models, …

Usu Lights Up The Twin Cities At National Women In Engineering Conference | College Of Engineering, Usu College Of Engineering

College of Engineering News

Utah State University was turning heads at the Minneapolis Convention Center last week at a large conference and expo for the Society of Women Engineers, known as SWE.

The Importance Of Small Fire Refugia In The Central Sierra Nevada, California, Usa, Erika M. Blomdahl, Crystal A. Kolden, Arjan J.H. Meddens, James A. Lutz

Wildland Resources Faculty Publications

Fire refugia – the unburned areas within fire perimeters – are important to the survival of many taxa through fire events and the revegetation of post-fire landscapes. Previous work has shown that species use and benefit from small-scale fire refugia (1 m² to 1000 m²), but our understanding of where and how fire refugia form is largely limited to the scale of remotely sensed data (i.e., 900 m² Landsat pixels). To examine the causes and consequences of small fire refugia, we field-mapped all unburned patches ≥1 m² within a contiguous 25.6 ha forest plot that …

Combating Alzheimer’S With 3-D Engineered Neuro Tissue | College Of Engineering, Usu College Of Engineering

College of Engineering News

Biological engineers at USU are developing new tools to combat the debilitating effects of Alzheimier’s and Parkinson’s disease and other brain disorders.

Engineers Without Borders: Team Mexico | College Of Engineering, Usu College Of Engineering

College of Engineering News

The USU student team, Engineers Without Borders, continues their water quality improvement project in rural Mexico.

Identification And Heterologous Reconstitution Of A 5-Alk(En)Ylresorcinol Synthase From Endophytic Fungus Shiraia Sp. Slf14, Huiwen Yan, Lei Sun, Jinge Huang, Yixing Qiu, Fuchao Xu, Riming Yan, Du Zhu, Wei Wang, Jixun Zhan

Biological Engineering Faculty Publications

A new type III polyketide synthase gene (Ssars) was discovered from the genome of Shiraia sp. Slf14, an endophytic fungal strain from Huperzia serrata. The intron-free gene was cloned from the cDNA and ligated to two expression vectors pET28a and YEpADH2p-URA3 for expression in Escherichia coli BL21(DE3) and Saccharomyces cerevisiae BJ5464, respectively. SsARS was efficiently expressed in E. coli BL21(DE3), leading to the synthesis of a series of polyketide products. Six major products were isolated from the engineered E. coli and characterized as 1,3-dihydroxyphenyl-5-undecane, 1,3-dihydroxyphenyl-5-cis-6'-tridecene,1,3-dihydroxyphenyl-5-tridecane, 1,3-dihydroxyphenyl-5-cis-8'-pentadecene, 1,3-dihydroxyphenyl-5-pentadecane and 1,3-dihydroxyphenyl-5-cis-10'-heptadecene, respectively, …

The Utah Statesman, October 23, 2018, Utah State University

The Utah Statesman

Weekly student newspaper of Utah State University in Logan.