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The Effects Of The Childbirth Process On The Attitudes And Behaviors Of New Fathers, Janice Ryser May 1981

The Effects Of The Childbirth Process On The Attitudes And Behaviors Of New Fathers, Janice Ryser

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Attitudes of 74 first-time fathers were studied as they varied vi by childbirth preparation and participation. A 50-variable questionnaire was used to tap fathers' attitudes towards their marital relationship and partner, their infants, themselves as fathers, and the pregnancy/childbirth experience.

Fathers agreed on being elated with fatherhood, wanting tactile, verbal, and extended home contact with their infants, planning on a partnership in parenting, and seeing their marriage at a high point. They saw pregnancy as a time of stress and adjustment and felt father participation was important. Their attitudes varied most on wife's performance and their own helpfulness through labor …


Stream Insect Production As A Function Of Alkalinity And Detritus Processing, Thomas G. Osborn May 1981

Stream Insect Production As A Function Of Alkalinity And Detritus Processing, Thomas G. Osborn

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The study was conducted to determine if aquatic insect production was significantly different between high and low alkalinity mountain streams and if any differences were associated with food availability factors. The major objectives included determining: (1) if annual production differences occur between high and low alkalinity streams; (2) if processing rates of terrestrial detritus differs between high and low alkalinity streams; (3) if detrital processing rates are related to stream insect productivities; (4) if primary productivity varies between high and low alkalinity streams; (5) if toxic effects or micronutrient limitations exist in high or low alkalinity streams that could limit …


Metaphorical Interpretations Of The Neurotic Paradox, Mark J. Weaver May 1981

Metaphorical Interpretations Of The Neurotic Paradox, Mark J. Weaver

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

This is a theoretical/philosophical paper which is intended to bring to the reader's attention an emerging literature and discussion which holds potentially productive consequences for the understanding of man. This thesis does not offer completed formulations or empirical groundings. The purpose is to create a basis for dialogue.

This paper will initially specify a current conflict in psychology around the different metaphors used to define the image of man. A theoretical/philosophical basis for viewing the process of generating models of man and his behavior as essentially "metaphorical" is then presented. A specific category of human behavior known as the neurotic …


Inter- And Intra-Sensory Modality Stimulus Scaling: A Method For The Determination Of The Relative Salience Of Stimuli In Poison-Based Aversion Learning By Pigeons, David L. Pounds May 1981

Inter- And Intra-Sensory Modality Stimulus Scaling: A Method For The Determination Of The Relative Salience Of Stimuli In Poison-Based Aversion Learning By Pigeons, David L. Pounds

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

One of the most rapidly expanding areas of research in psychology has been poison-based aversion learning (PBAL). The PBAL paradigm typically involves: exposing an animal to a novel substance; inducing illness following ingestion of that substance; and then providing access to the substance at a later time. The initial reaction to the novel substance is generally to reduce consumption, a finding labeled neophobia. The reduction of substance intake on test day is called learned aversion.

Following demonstrations of cue-to-consequence specificity (i.e., the differential associability of some stimuli with certain consequences) in PBAL research with rats, recent research has focused on …


Marital Roles And Their Relationship To Marital Happiness And Self Concept, Gary L. Devries May 1981

Marital Roles And Their Relationship To Marital Happiness And Self Concept, Gary L. Devries

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Marital roles have become a subject of major concern in recent years. Many critiques consider traditional marriage roles to be responsible for hindering appropriate social-emotional development of the wife, in particular, and also the husband. Past research in assessing the relationship between marital roles and the happiness and well-being of husbands and wives is limited in quantity and generally is inconclusive or controversial. This study was designed to clarify the relationship between marriage roles and two dependent variables, marital happiness and self concept.

The sample population consisted of 124 volunteer couples selected from the teaching staffs of eight school districts …


Effect Of Toxaphene On Collagen Synthesis In Fish Tissue: Organ Culture Studies And Prolyl Hydroxylase Activity Assay, Charles Franklin Luke May 1981

Effect Of Toxaphene On Collagen Synthesis In Fish Tissue: Organ Culture Studies And Prolyl Hydroxylase Activity Assay, Charles Franklin Luke

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Toxaphene is reported to cause defects in the collagen of fish. Chronic exposure to toxhaphene weakens the backbone of fish by decreasing the amount of collagen and usually increasing the amount of calcium in the bone which results in a more brittle and fragile bone.

We investigated the possible direct action of toxaphene on collagen synthesis by exposing vertebral and swim bladder organ cultures obtained from unexposed rainbow trout (Salmo gairdneri) fingerlings to the same lot of toxaphene found to cause this defect in vivio. Collagen produced by these organ cultures was measured by: (1) total 3 …


Influence Of Lipophilicity On The Accumulation And Distribution Of Halogenated Phenols And A Pyridinol As Metabolites Of Pesticides In The Rat, Assed A. Attumi May 1981

Influence Of Lipophilicity On The Accumulation And Distribution Of Halogenated Phenols And A Pyridinol As Metabolites Of Pesticides In The Rat, Assed A. Attumi

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Exposure to halogenated phenols and pyridinols is of increasing concern because of their wide use and distribution. This research was initiated to determine the distribution, accumulation, and depletion of a group of halogenated phenols and a pyridinol in selected tissues of male weanling rats at different time intervals following a single oral dose of 0.33 or 1.66 m moles per kg body weight. The halogenated phenols and pyridinol were distributed differently in every tissue sampled following their administration, even though the amount administered was the same in each case. The concentrations in tissue were found in the order: 2,4,5-trichlorophenol > 4-bromo-2,5-dichlorophenol …


Models For Estimating Energy And Protein Utilization For Feeds, Muhammad Fadel Wardeh May 1981

Models For Estimating Energy And Protein Utilization For Feeds, Muhammad Fadel Wardeh

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Data on the proximate nutrient content of feedstuffs, digestibility and energy utilization available from the International Feedstuffs Institute (Utah State University) were used to develop mathematical models for estimating energy and protein utilization of five classes of feedstuffs for various kinds of animals.

Classes of feedstuffs were subdivided into more related subclasses. Furthermore, data from all feeds were pooled together then subgrouped into more related subgroups in an attempt to gain high precision in prediction of digestible proximate nutrients and TDN from a single chemical entity by the use of simple regression models (Y = bo + b1x …


Biphenyl Hydroxylation By Vole Hepatic Microsomes, Moheb H. Makary May 1981

Biphenyl Hydroxylation By Vole Hepatic Microsomes, Moheb H. Makary

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Metabolism of biphenyl by meadow voles (Microtus montanus) liver microsomes was studied by gas liquid chromatography. The microsomal biphenyl hydroxylation of Swiss Webster mice (outbred) was also investigated to provide a species comparison. 4-hydroxybiphenyl (major metabolite), 3-hydroxy biphenyl (minor) and smaller amounts of 2-hydroxybiphenyl were identified as metabolites of biphenyl incubated with vole liver microsomes. Liver microsomes from Swiss mice formed 4-hydroxy biphenyl (major metabolite) and almost equal amounts of 2- and 3-hydroxy biphenyl (minor metabolite). The amount of the hydroxy biphenyls formed by vole liver microsomes was considerably less per milligram of microsomal protein than for mice. …


Petrology Of The Middle Cambrian Blacksmith Formation, Southeastern Idaho And Northernmost Utah, David Paul Zelazek May 1981

Petrology Of The Middle Cambrian Blacksmith Formation, Southeastern Idaho And Northernmost Utah, David Paul Zelazek

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The Blacksmith Formation of Middle Cambrian (Albertan) age was studied in southeastern Idaho and northernmost Utah. Lithology and sedimentary structures were compared with modern environments and ancient rocks to determine environments of deposition, paleo-geography, diagenetic alterations and patterns of dolomitization.

The Blacksmith Formation can be divided into five basic rock types. Rock type A has cryptalgalaminae, mudcracks, and lacks bioturbation. Rock type A is inferred to have been deposited in the upper intertidal to supratidal environment. Rock type B is extremely dolomitized and brecciated. It is inferred that rock type B was deposited in the upper intertidal to supratidal environment. …


Seasonal Temperature Preference Of Adult Mountain Whitefish, Prosopium Williamsoni, Jean M. Ihnat May 1981

Seasonal Temperature Preference Of Adult Mountain Whitefish, Prosopium Williamsoni, Jean M. Ihnat

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Temperatures selected seasonally by adult mountain whitefish were measured in the laboratory in a horizontal gradient. Final preferendum estimates, based on acute (3-hour) preference tests conducted with fish acclimated to 5, 10, and 15 C each season, were 17.7 C (pre-spawning), 11.9 C (post-spawning), 9.9 C (winter), and 16.3 C (spring). Seasonal influence on temperature selection was evident on the basis of differences in final preferenda, covariance analysis of responses of laboratory-acclimated fish, and temperature selection by fish held at ambient river temperatures. Post-spawning and winter groups selected lower temperatures than did pre-spawning and spring groups. Pre-spawning fish selected temperatures …


Some Morphological And Chemical Responses Of Blackbrush (Coleogyne Ramosissima) To Goat Browsing: Influences On Dietary Blackbrush Selection By Goats And Cattle, Frederick D. Provenza May 1981

Some Morphological And Chemical Responses Of Blackbrush (Coleogyne Ramosissima) To Goat Browsing: Influences On Dietary Blackbrush Selection By Goats And Cattle, Frederick D. Provenza

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Domestic goats were used to modify the growth form of blackbrush, a spinescent shrub occurring in nearly monospecific stands on several million hectares of rangeland in the southwestern United States. The objective of this research was to evaluate goat browsing as a means of improving these rangelands for cattle. Winter goat browsing stimulated spring twig growth from basal and axillary buds which resulted in increased production.

Twig production by heavily browsed plants (>95 percent removal of current season's twigs) was a function of precipitation, soil depth, branch location on the plant, and period of rest after browsing. As precipitation …


Natural Pastures Of The Macquarie Region Of New South Wales: Their Origin, Composition And Management, David Leslie Michaelk May 1981

Natural Pastures Of The Macquarie Region Of New South Wales: Their Origin, Composition And Management, David Leslie Michaelk

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Two experiments were conducted in the semi-arid (400 millimeter annual rainfall) Macquarie region of New South Wales, Australia, at the Trangie Agricultural Research Station (31° 59'S; 147° S7'E), to examine (a) the way botanical parameters can be used to separate grazing and climatic impacts on range vegetation, and (b) how this delineation affects application of range science tenets (site, condition and trend) to different pasture types.

Two range sites were studied: Site 1, a light soil type, was dominated by annual grasses, legumes and forbs, whereas perennial grasses (mainly Chloris acieularis and Chloris truncata) dominated the heavy soils of …


Economic Analysis Of Long-Term Management Strategies For Two Sizes Of Utah Cattle Ranches, Roger E. Banner May 1981

Economic Analysis Of Long-Term Management Strategies For Two Sizes Of Utah Cattle Ranches, Roger E. Banner

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Utah cattle ranchers realize relatively little profit from ranch ownership and management. This study represents an attempt to identify ranch management strategies that produce more profit over time than do conventional strategies.

To identify optimum management strategies for the long term, analyses of ranches under both normal and adverse ranch operation conditions using the COPLAN linear programming model were made for strategy comparison. To depict these ranch business environmental conditions, production levels were estimated from available biological data and price levels were estimated by indexing 1977 ranch product prices (the most current budget data available for Utah). The variability of …


Diversity And Production Of Herbaceous Vegetation In A Northern Utah Subalpine Chronosequence, Gary A. Reese May 1981

Diversity And Production Of Herbaceous Vegetation In A Northern Utah Subalpine Chronosequence, Gary A. Reese

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Successional trends in herbaceous plant production and diversity were studied in an age sequence of sites, i.e. chronosequence, inferred to represent a meadow to aspen to fir to spruce-fir sere. Primary production was observed to decrease in a linear fashion with successional development. Three components of diversity; richness, heterogeneity, and equitability or evenness, each had low early successional values, reaching maximum diversity in mid-succession, and declining to intermediate levels with maturity. The magnitude of these trends varied greatly, depending on the methods used to determine plant dominance. Characteristics of various dominance indices and their applicability to this study were examined. …


Effect Of Mild Water Stress And Enhanced Ultraviolet-B Irradiation On Leaf Growth Of Rumex Obtusifolius L. And Rumex Patientia L. (Polygonaceae)., Steve R. Holman May 1981

Effect Of Mild Water Stress And Enhanced Ultraviolet-B Irradiation On Leaf Growth Of Rumex Obtusifolius L. And Rumex Patientia L. (Polygonaceae)., Steve R. Holman

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Leaves of Rumex obtusifolius L. and R. patientia L.were exposed to combinations of mild water stress and enhanced ultraviolet-B irradiation during their ontogeny. Two UV-B treatments (enhanced UV-B and control) and three water stress treatments (-0.0 MPa, -0.2 MPa and -0.4 MPa rooting medium matric potentials) were employed. The impact of the stress interaction was assessed on the basis of changes in leaf area, average adaxial epidermal cell size, and total number of adaxial epidermal cells per leaf. Although the level of UV-B irradiation applied was insufficient to significantly alter leaf growth at any given water stress, UV-B did interact …


The Energy Expenditure Of Heifers Grazing Crested Wheatgrass Rangeland In West-Central Utah, Kris M. Havstad May 1981

The Energy Expenditure Of Heifers Grazing Crested Wheatgrass Rangeland In West-Central Utah, Kris M. Havstad

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The free-roaming ruminant requires energy for the demands of vii grazing, traveling and thermoregulation that are not required by its confined counterpart. Literature estimates of these additional costs range from 10 to 170 percent above maintenance. The uncertain magnitude of this increased demand and the factors that contribute to it impede the ability of the rangeland ruminant nutritionist to establish guidelines for the energy requirements of the free-roaming herbivore. This study was designed to estimate the energy expenditure of yearling Angus heifers while grazing a declining supply of available crested wheatgrass forage (Agropyron cristatum) on rangeland in west-central …


Lifestyle As A Determinant Of Participation Among Dispersed Forest Recreationists, John R. Butler May 1981

Lifestyle As A Determinant Of Participation Among Dispersed Forest Recreationists, John R. Butler

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

This study assesses the usefulness of lifestyle as a determinant of outdoor recreation behavior. Two objectives of the study are: (1) develop an operationalized concept of lifestyle that is based on theory, and (2) apply this concept in a model which uses lifestyle as a variable influencing recreation behavior.

The first objective was approached through an integrative review of the literature. Lifestyle was set in context of cultural theory. The basic postulate of the proposed theory of lifestyle is: If an individual's lifestyle is similar to that of another, certain social psychological processes are similar. Therefore, their patterns of needs, …


Simulated Use Of The Exponential Polynomial/Maximum Likelihood Technique In Developing Suitability Of Use Functions For Fish Habitat, Kenneth A. Voos May 1981

Simulated Use Of The Exponential Polynomial/Maximum Likelihood Technique In Developing Suitability Of Use Functions For Fish Habitat, Kenneth A. Voos

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The purpose of this paper is to present a new methodology for producing fish habitat suitability at use functions. The suitability functions are relative measures of the voluntary or involuntary preference for combinations of the values of environmental attributes by a fish species and age class. This suitability function is used to determine the relative usability of a physical stream environment as fish habitat.

A generalized function form, exponential polynomials, was selected for the development of these suitability of use functions. The maximum likelihood technique was used for function parameter solution. Derivation of the suitability functions was simulated for several …


Crown And Root Rot Of Alfalfa In Utah, Victoria Allard Turner May 1981

Crown And Root Rot Of Alfalfa In Utah, Victoria Allard Turner

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Symptoms of dark crown necrosis were found in all fields and varieties of alfalfa examined throughout the state. No significant differences were noted among varieties in 1 year old test plots in the amount of disease (Disease Index = D.I.) present in crown tissue. Significant differences were noted in plots 2.4 years and older among the different varieties. Varieties common to all plots did not differ overall; however, varieties responded differently according to location.

Fusarium spp. were most frequently isolated from affected crowns throughout the state, yet, differences in occurrence of species among the different aged plots and fields were …


Impact, Epidemiology And Control Of Crown And Root Rot Of Spring Barley In Utah, Rastri Dey Kidambi May 1981

Impact, Epidemiology And Control Of Crown And Root Rot Of Spring Barley In Utah, Rastri Dey Kidambi

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

A three year study was initiated to study impact, epidemiology and control aspects of crown and root rot of irrigated and dryland spring barley in Utah. Isolations from leading edges of necrotic lesions of infected plants showed Cochliobolus sativus to be most frequently isolated. Yield loss studies showed that the disease had it's greatest effect in kernel weight reduction.

Time course studies of disease initiation, development and progress were made throughout two consecutive growing seasons. Disease initiation began 25-30 days after planting and reached maximum levels at approximately 60-80 days. Soil inoculum levels were determined by the flotation viability method …


Hydraulic Conductivity And Drought Acclimation Of Cotton Root Systems, Derrick M. Oosterhuis May 1981

Hydraulic Conductivity And Drought Acclimation Of Cotton Root Systems, Derrick M. Oosterhuis

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Hydraulic conductivity of single roots and whole root systems was studied for soil, sand and solution-grown cotton plants (Gossypium hirsutum L.). Conductivity varied widely depending on plant age and growing conditions. Total conductivity for soil-grown plants decreased from 357 x 10-6 µ1 s-1 bar-1 cm-1 one week after germination to 5 x 10-6 µ1 s-1 bar-1 cm-1 at boll set. There was a concomitant increase in total root length. Solution-grown plants had higher root conductivities than soil- or sand-grown plants. Root conductance was decreased by low temperatures, lack of oxygen and …


Examination Of The Effect Of Physical Exercise On Metabolic Control In Type I Insulin-Dependent Youth With Diabetes Mellitus, Gayle Ann Brazzi May 1981

Examination Of The Effect Of Physical Exercise On Metabolic Control In Type I Insulin-Dependent Youth With Diabetes Mellitus, Gayle Ann Brazzi

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

There is evidence for the importance of physical exercise balanced with diet and insulin therapy in the treatment and control of Type I insulin-dependent diabetes mellitus. Recent attention in the literature has focused on the once neglected component of treatment--exercise. A well-controlled exercise state is generally believed to be beneficial for the individual with diabetes. Limited studies are available on the effect of exercise on long-term metabolic control. Before exercise levels can be professionally prescribed for a given population of youth with diabetes, current exercise patterns should be evaluated as to the effect on long-term metabolic control.

It is the …


An Investigation Of The Rework Defect In Process Cheese, Patricia Ramirez Baca May 1981

An Investigation Of The Rework Defect In Process Cheese, Patricia Ramirez Baca

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The effect of time that processed cheese was held ex- posed to heat alone or with agitation, the level of rework cheese, and the type and level of emulsifying salt were evaluated by determining finess of emulsion based on scanning electron microscopy measurements, meltability, and rheological measurements using a Universal Testing Machine.

Process cheese held in the cooker at 82°C for up to 4o minutes became less meltable and more firm and the emulsion became finer while that held at 82°C outside of the cooker without agitation was only slightly affected. Loss of meltability and increased firmness associated with pro-longed …


Effect Of Atmospheric Oxidation On Bioavailability Of Meat Iron And Liver Weights In Rats, Kathleen M. Cardon May 1981

Effect Of Atmospheric Oxidation On Bioavailability Of Meat Iron And Liver Weights In Rats, Kathleen M. Cardon

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Iron bioavailability of diets containing oxidized turkey and oxidized beef meat was investigated in two experiments. In both studies male, weanling rats were made anemic by consumption of a low iron diet and bleeding. The experimental diets were prepared so that meat was the only source of iron. Hemoglobin regeneration served as the basis for measuring iron utilization. In experiment 1, lyophilized, uncooked turkey meat was allowed to oxidize at 20-22°C for 0, 48, 96, 144, 216, or 264 hours and then fed to the rats. The length of oxidation time of the turkey meat did not significantly affect the …


Bovine Muscle Cathepsin D: Purification And Proteolytic Activity On Muscle Proteins, Paul Hwaleun Fan May 1981

Bovine Muscle Cathepsin D: Purification And Proteolytic Activity On Muscle Proteins, Paul Hwaleun Fan

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

An affinity column for cathepsin D was prepared making use of the strong affinity of pepstatin for cathepsin D. Pepstatin is an N-acylated pentapeptide from Actinomycetes with the following structure: isovaleryl-L-valyl-L-valyl-4-amino-3-hydroxy-6-methylheptanoyl-L-alanyl-4-amino-3-hydroxy- 6-methyl heptanoic acid. A relatively rapid and efficient method for cathepsin D purification has been developed; Steps include homogenization, ammonium sulfate fractionation, and chromatography on pepstatin-Sepharose column. The final preparation has a specific activity of 38 units/mg. and shows a single protein band on polyacrylamide gel electrophoresis in sodium dodecyl sulfate corresponding to a subunit molecular weight of 42,000. Polyacrylamide gel electrophoresis studies did not reveal any impurities. The …


Bioavailability Of Calcium, Fluoride And Iron In Mechanically-Deboned Beef Fed To Growing Rats, Kathryn Mclaughlin May 1981

Bioavailability Of Calcium, Fluoride And Iron In Mechanically-Deboned Beef Fed To Growing Rats, Kathryn Mclaughlin

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Weanling rats were fed diets containing raw lyophylized hand-deboned shank beef (HDS) supplemented with CaCO3, retorted lyophylized mechanically deboned shank beef (MDS), or raw lyophylized MDS. Ca in the latter two diets originated from bone during the mechanical deboning process. Ca absorption, Ca retention, bone weight, and bone breaking strength were similar for comparative dietary levels of Ca regardless of the source. Bone ash and Ca content was significantly higher (P < .05) in the MDS 393 (3.93 g Ca/kg diet) diet and in the retorted MDS 460 diet compared with the HDS 488 diet. Relative biological values for the MDS diets calculated relative to the HDS diets ranged from 102 to 132 when the linear regression of Ca consumed, Ca retained, or dietary Ca level vs bone ash or bone Ca content was obtained. The retorted MDS 460 diet contained the highest Fe level and exhibited a lower percent absorption, terminal hemoglobin level, and liver Fe storage. Humerus F content increased as dietary F (also present from bone) level increased. Dietary F level did not determine humerus breaking strength.


Factors Affecting The Measurement Of Undenatured Whey Protein Nitrogen In Dried Whey By A Modified Harland-Ashworth Test, Reyad M. Aboumahmoud May 1981

Factors Affecting The Measurement Of Undenatured Whey Protein Nitrogen In Dried Whey By A Modified Harland-Ashworth Test, Reyad M. Aboumahmoud

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The Harland-Ashworth test for measuring undenatured whey protein nitrogen in nonfat dry milk was modified to measure undenatured whey protein nitrogen in dried whey. The new test was designed to measure absorbance in Harland-Ashworth filtrates at 280 nm to eliminate difficulties associated with turbidimetric measurements. One gram dried whey was reconstituted in 25 ml pH 6.7 phosphate buffer (.1M) in a 25 x 150 mm test tube. Ten grams sodium chloride were added. The test tubes were incubated for 30 min in a water bath at 37C and shaken 8-10 times in the first 15 min. The tubes were removed …


An Enzyme-Linked Immunosorbent Assay (Elisa) For Pantothenate, Allen H. Smith May 1981

An Enzyme-Linked Immunosorbent Assay (Elisa) For Pantothenate, Allen H. Smith

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

An enzyme-linked immunosorbent assay (ELISA) for pantothenate has been developed. Antibodies induced in rabbits against bovine serum albumin-pantothenate conjugate were specifically purified by affinity chromatography. This process served to reduce the amount of endogenous pantothenate attached to the antibody, as well as to purify the antibody. The purified antibodies were covalently linked to alkaline phosphatase (Sigma type VII) with glutaraldehyde (0.05% aqueous solution). An immobilized pantothenate substrate was first obtained by attaching human serum albumin-pantothenate conjugate to the surface of polystyrene culture tubes by passive adsorption. The binding of the enzyme labelled antibody (E-AB) to this substrate is proportionately inhibited …


Vitamin A And Vitamin E Status Of Persons On Hemodialysis, Kathleen S. Johnson May 1981

Vitamin A And Vitamin E Status Of Persons On Hemodialysis, Kathleen S. Johnson

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The study was designed to assess the vitamin A and vitamin E status of persons receiving maintenance hemodialysis. Blood samples from thirty persons receiving hemodialysis, as well as age and sex-matched controls, were analyzed for vitamin A, vitamin E, and carotene using a spectrofluorometric assay for vitamins A and E, and a spectrophotometric assay for carotene. Dietary intakes of vitamins A and E were estimated via verbal questioning. Samples of dialysate were also studied. Results of the investigation showed significantly elevated vitamin A, normal vitamin E, and decreased carotene levels in persons with renal failure receiving maintenance hemodialysis, when compared …