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Fibroblast And Epidermal Cell-Type I Collagen Interactions: Cell Culture And Human Studies, Charles J. Doillon, Frederick H. Silver, Robert M. Olson, Chandrakala Y. Kamath, Richard A. Berg

Scanning Microscopy

Fibroblast and epidermal cell-type I collagen sponge interactions were studied in cell culture as well as in humans. In cell culture, fibroblasts were observed to migrate and proliferate throughout a type I collagen sponge containing either hyaluronic acid (HA) or fibronectin (FN). Fibroblasts accumulated in the center of the pores in sponges containing HA and appeared to surround themselves with newly synthesized extracellular matrix. In sponges containing FN, fibroblasts attached to and elongated along the collagen fibers of the sponge. In the absence of FN or HA protein synthesis of fibroblasts appeared to be inhibited by the presence of the …

The Role Of Scanning Electron Microscopy In Periodontal Research, A. Carrassi, S. Abati, G. Santarelli

Scanning Microscopy

During recent years a great amount of research has led to a better understanding of the etiology, pathogenesis and pattern of progression of periodontal diseases. Scanning electron microscopy (SEM) has contributed to this improvement, mainly with respect to histology of periodontal tissues, the description of the morphology and distribution of bacteria on the exposed root surface, analysis of the host-parasite interactions on the gingival pocket wall, and morphological evaluation of root treatment. This review deals with all these topics. Unusual types of SEM research are also described and discussed. Uncommon sample preparation techniques for SEM in periodontal research are described. …

Ion And Water Retention By Permeabilized Cells, Carlton F. Hazlewood, Miklos Kellermayer

Scanning Microscopy

Nonionic detergents, Triton X-100 and Brij 58, removed, within 2-5 minutes, lipid membrane of suspended thymus lymphocytes and monolayer H-50 cells grown in culture. Studies of hydration, ionic asymmetry, and ionic and protein release kinetics were conducted on these membraneless cellular preparations. The hydration of nuclei isolated by Triton X-100 procedures appears to be influenced strongly by the monovalent ionic concentration of the buffer bathing the organelles. The putative monovalent ionic concentration of the cellular aqueous phase (i.e., 150 meq/L) caused nuclei to swell and coalesce. Monovalent ionic concentrations of 30 meq/L or less caused minimal changes in volume and …

Surface Morphology Of Human Airway Mucosa: Normal, Carcinoma Or Cystic Fibrosis, P. K. Jeffery, A. P. R. Brain

Scanning Microscopy

The study presents preliminary qualitative findings of an investigation of grossly normal main and lobar bronchi at sites distant to well circumscribed tumour (n=15), adjacent to tumour (n=5) or of airways obtained during heart/lung transplantation in patients with cystic fibrosis (CF, n=3). In the normal airways the surface epithelium was on average 50 𝝁m thick, pseudostratified and rested on a roughly contoured basement membrane. A variety of cell types were identified although many were obscured by a dense covering of cilia, occasionally interrupted by foci of squamous metaplasia. Submucosal gland structure was observed in chance vertical fractures of the airway …

Scanning Electron Microscopy Of Oral Mucosa In Vivo And In Vitro: A Review, Kristina Arvidson, Roland C. Grafström, Anders Pemer

Scanning Microscopy

The oral mucosa is classified by function into lining, masticatory and specialized oral mucosa, with regional structural adaptation. In this review, the surface structures of the human oral mucosa have been studied in the scanning electron microscope (SEM). Regional variations in regard to keratinization, cell arrangements and microplications with related specific structures observed in SEM are described and correlated with the appearance of similar areas observed in the light microscope. Furthermore, human oral tissue and cell cultures have also been studied. These systems offer usable and complementary models for performing similar studies in vitro under controlled experimental conditions. We now …

The Role Of The Cytoskeleton In Endothelial Repair, Avrum I. Gotlieb, Michael K. K. Wong, Patricia Boden, Alan Choo Fone

Scanning Microscopy

The injured endothelium undergoes rapid repair of areas of cell desquamation in order to maintain the structural integrity of the endothelial surface. Endothelial repair involves a series of processes which include endothelial cell spreading, translocation, and proliferation. These processes are well defined events which occur sequentially in time. Spreading and translocation are mediated by the cell cytoskeleton - F-actin microfilaments and microtubules and associated centrosomes. The regulation of these processes is complex and is likely due to soluble factors present at the site of injury which are released from activated endothelial cells, platelets, the subendothelial substratum, activated serum factors, and …

Scanning Electron Microscopy Of Black Stain On Human Permanent Teeth, J. Theilade, K. M. Pang

Scanning Microscopy

Black stain may develop on the coronal surfaces of human teeth, and this type of stain is common in the Hong Kong Chinese population. The present study was undertaken to ascertain if the deposit conforms in composition to the black stain found elsewhere, and to describe its ultrastructure using the scanning electron microscope. Gram-stained smears were made from black stain on the teeth of 11 adult Hong Kong Chinese and studied microscopically. From another 15 persons extracted teeth exhibiting black stain were obtained and fixed. Two ground sections were made from each tooth, one was stained with toluidine blue, the …

Ultrastructural Analysis Of Enamel Formation During In Vitro Development Using Chemically-Defined Medium, P. Bringas Jr., M. Nakamura, E. Nakamura, J. Evans, H. C. Slavkin

Scanning Microscopy

To test the hypothesis that enamel biomineralization is regulated by sequential expression of an intrinsic genetic program, we designed experiments to determine if a serumless, chemically-defined medium is permissive for position-dependent ameloblast differentiation and subsequent enamel tissue-specific biomineralization in vitro. In the absence of serum or other exogenous growth factors, Swiss Webster strain mouse embryonic (15-and 16-days gestation) mandibular first molar tooth organs (cap stage) developed within 21 days in vitro into well-defined molar tooth organs expressing dentine and enamel biomineralization. Analysis of data obtained from von Kossa histochemistry for calcium salt formation, as well as ultrastructural information obtained …

Effects Of Drought On The Survival Of Rhizobium Leguminosarum Biovar Trifolii And The Nodulation Of Subterranean Clover In An Acid Soil, Carmen Bueno

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Twenty-nine Rhizobium leguminosarum biovar trifolii strains were tested for acidity tolerance in acidified liquid medium. Only 41% of the strains grew at pH 4.1. One acid-tolerant strain, USDA 2160, and one acid-sensitive strain, 162-X-103 from Nitragin Co., were inoculated on seeds of 'Nungarin', 'Seaton Park' and 'Clare' subclover cultivars. The inoculated and pelleted seeds were sown in potted Cluff soil with pH 5.7. Three desiccation levels were imposed by delaying watering for 0, 15 or 30 days. Four gravimetric soil water contents (6.0, 6.6, 10.5 and 12.5%) were maintained under a greenhouse line-source sprinkler system for 7 weeks. The desiccation …

Honoring The Farm: Identity And Meaning In Personal Narratives, Jeannie Banks Thomas

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

This thesis employs the literary folklorist methodology to explore personal narratives. Personal narratives told by Elizabeth (Beth) Wyatt Winn were analyzed. It was discovered that these narratives provide an eyewitness account of history, reveal world views, and encapsulate experiences into values and personal meanings. The depth of meaning found in Elizabeth (Beth) Wyatt Winn's personal narratives illustrates the importance of personal narratives in historical research and historical re-creation and simulation.

Appendices include several oral interviews containing personal narratives.

Exploring Folk Art, Michael Owen Jones

All USU Press Publications

Jones explores the human impulse to create, the necessity for having aesthetically satisfying experiences, and the craving for tradition. He also considers topics such as making chairs, remodeling houses, using and preserving soda-fountain slang, preparing and eating food, and sculpting lifelike figures out of cement.

Purification Of Anthrax Toxin Protective Antigen Component And Characterization Of Its Binding Interaction With Bovine Kidney Cells, Daniel Dalton Martin

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Protective antigen component of B. anthracis toxin was produced and purified to the >99% level. Toxin was purified from culture supernatant utilizing concentration and liquid chromatography techniques. Purity was determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis.

The purified protective antigen retained biological and antigenic activity as evidenced respectively by lethality in Fischer 344 rats when injected in combination with lethal factor, and by positive results on the Ouchterlony double diffussion assay.

Radioiodinated protective antigen was used both in the in vivo and the in vitro experiments.

In vivo distribution of labelled protective antigen was determined in Fischer 344 rats. Assay …

Cable Television Utilization In Logan, Utah, Kathryn Whittle

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The recent expansion in the cable television industry has brought about a kind of media revolution. Many more types of programs are being made available to the public via cable than would have been possible through regular network broadcasting.

Folk Art And Art Worlds, John Michael Vlach, Simon J. Bronner

All USU Press Publications

Respected voices combine in this volume to address the fundamental issues pertaining to American folk art. The eleven original essays explore the changing face of folk art in its different contexts or folk art "worlds"--the worlds of the creator, the academic, the art dealer, the gallery or museum, and the community. A new Introduction considers supplemental works on American Folk Art that have appeared since the volume was first published.

Survival And Growth Of Adult Human Oligodendrocytes In Culture: Scanning Electron Microscopy, Seung U. Kim, Kookmin M. Kim, Giuseppe Moretto, Doo H. Shin

Scanning Electron Microscopy

The oligodendrocytes in culture serve as an important model for the study of demyelination diseases. We have previously originated a method of isolating human oligodendrocytes. In order to establish their three dimensional morphology, scanning electron microscopy of the cultured oligodendrocytes was performed.

The oligodendrocytes bulk isolated from adult human brain were maintained in culture for more than 2 months. At the various periods in culture, the cells were studied by scanning electron microscopy and immunofluorescence staining using marker antibodies for the identification of oligodendrocytes. The three dimensional organization and the surface morphology of the cultured oligodendrocytes were investigated. They displayed …

Problems Associated With The Preparation Of Cell Suspensions For X-Ray Microanalysis Highlighted By The Comparison Of Results With Those Obtained From Tissue Sections, A. Warley, M. D. Kendall, I. W. Morris

Scanning Electron Microscopy

Fully quantitative X-ray microanalysis of freeze dried frozen sections allowed the concentration of monovalent ions in the nuclei of mouse thymocytes in tissue sections to be compared with those from thymocytes which had been isolated by a simple preparation procedure. Isolated cells showed increased concentrations of Na and Cl. This was further investigated in the rat where the size of the thymus a 11 owed comparison between different isolation media using cells derived from the same animal. Use of autologous serum as the final suspending medium gave reproducible results, whereas with suspension in BSS, or 20% dextran the results were …

Fluoride Effects On The Growth And Morphology Of Two Thermophilic Cyanophytes Synechcoccus Lividus And Phormidium Laminosum, Ibrahim A. Arif

Biology

The effects of various concentrations of fluoride on growth of two thermophilic cyanophytes, Synechococcus lividus (unicellular) and Phormidium laminosum (filamentous) were studied. Growth of both algae was inhibited by fluoride in the concentrations tested. As the concentrations of fluoride increased, an extended adaptation lag phase was encountered. Synechcoccus lividus was more sensitive to fluoride than was Phormidium laminosum, being inhibited in total growth as well as growth rate. Fluoride effects on growth were compared to chloride and to chloride plus fluoride. The growth of S. lividus was stimulated by chloride alone and in combination with fluoride at all concentrations used; …

Infection Of Oriental Mustard By Nematospora: A Fluorescence And Scanning Electron Microscope Study, Richard A. Holley, Beverley E. Phipps-Todd, Suk H. Yiu

Food Structure

Fluorescence light microscopy and scanning electron microscopy were used to study penetration by the yeast Nematospora caryli through the seed coat and into the embryonic tissues of oriental mustard seed (Brasslca iuncea).

Infection of the seed was associated with its physical injury; however, it was evident that the yeast was capable of successfully invading healthy plant cells. The pathological process was followed in parallel using both the above types of microscopy. Foci of yeast infection on the seed coat outer surface were characterized by swelling of the infected epidermal cells. Nematospora hyphae were seen in the lumina of the seed …

A Mass Media Campaign To Promote Divorce Adjustment, James C. Gardiner

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Introduction. This study produced and evaluated a mass media campaign designed to promote emotional adjustment to divorce.

Hypotheses. (a) Sending a promotional newsletter to divorced persons will increase their reported utilization of the campaign. (b) Divorced persons who report heavy utilization of the campaign will report greater emotional improvement than divorced persons who report light or no utilization of the campaign.

Method. A field experiment was conducted in rural northern Utah. The names of all recently (less than 12 months) divorced persons were obtained from the county clerk and randomly divided into an experimental group, who received a newsletter promoting …

Electron Microscopic Observation Of The Development Of A Bovine Rotavirus In Madin-Darby Bovine Kidney Cells, Corey H. Mjaatvedt

Biology

The purpose of this study was to describe some of the ultrastructural events which occur in Madin-Darby bovine kidney cells infected with bovine rotavirus. Several observations were made in this study which have not been previously reported. First, distension of the rough endoplasmic reticulum cisternae was observed to occur before the entry of virus particles. Second, an increase in ribosome-like particles in the cytoplasm was seen to occur. Third, bundles of microfilaments were often seen near the nuclear envelope, mitochondria, and the rough endoplasmic reticulum. Fourth, two different densities of viroplasm were observed. Fifth, progeny virus particles were observed in …

Time Spent In Home Care Tasks Related To Ownership And Uses Of Home Care Equipment, Jeena C. H. Nilson

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The data for this research were taken from Utah's contribution to the regional research project "An Interstate Comparison of Urban/Rural Families' Time Use." Data were collected between May 1977 and August 1978 from 210 two-parent/ two-child families.

This thesis research studied the relationship between ownership and use of nine selected household appliances and time spent on the related housekeeping tasks for 208 of the families studied. Statistical analysis was done using t-tests for comparisons of time spent on the related task by owners and non-owners of each appliance. Analysis of variance was used to compare time spent on combined activities …

Effect Of Toxaphene On Collagen Synthesis In Fish Tissue: Organ Culture Studies And Prolyl Hydroxylase Activity Assay, Charles Franklin Luke

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Toxaphene is reported to cause defects in the collagen of fish. Chronic exposure to toxhaphene weakens the backbone of fish by decreasing the amount of collagen and usually increasing the amount of calcium in the bone which results in a more brittle and fragile bone.

We investigated the possible direct action of toxaphene on collagen synthesis by exposing vertebral and swim bladder organ cultures obtained from unexposed rainbow trout (Salmo gairdneri) fingerlings to the same lot of toxaphene found to cause this defect in vivio. Collagen produced by these organ cultures was measured by: (1) total ^{3 …}

Socio-Demographic And Economic Factors Affecting Fertility In Rural And Urban Thailand, Pichit Pitaktepsombati

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The major purpose of this study is to measure the relationship between socio-demographic and economic factors and fertility of rural and urban women in Thailand, utilizing national level survey data collected in 1972 and 1973. Specifically, a regression model of fertility and socio-demographic and economic variables will be developed and analyzed. Also, a general comparison will be made between the results of the present study, based on the 1972 and 1973 surveys, with those from an earlier round of surveys conducted in 1969 and 1970.

Comparison Of Tolerance To High Moisture Conditions Of The Soil Among Crop Plants: Studies On The Comparative Plant Nutrition [English Translation], Toshiaki Tadano, Kiyokazu Kirimoto, Isao Aoyama, Arika Tanaka

Publications

Tolerance to high moisture conditions in the soil was compared for eighteen crop species, and factors causing differences among species were studied: 1) When the moisture content of a soil was kept high, the soil air contained 10.5 - 17.5% of O2 and 5.0 - 9.8% of CO2, and the soil solution was not very high in Fe, Mn or NO2-N. 2) Cruciferae, Solanaceae, sugar beet, field bean, pea, cucumber and Chrysanthemum coronarium were very susceptible to this condition, but maize, soybean, and onion were tolerant. Rice grew better under this condition than under ordinary upland conditions. 3) Tolerance to …

Religious And Geographic Correlates Of Breast Feeding, Joan Boyington Sellers

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

This study focused mainly upon regional and religious variables influencing a mother's decision for a particular infant feeding practice. The problem of this study was to investigate the breast feeding and maternal attitudes of Mormon and non-Mormon mothers who were raised within the Mountain States region and compare them to the attitudes of Mormon and non-Mormon women who are not residents of this region.

No significant differences were found between the resident and non- resident women in their practice of bottle or breast feeding, nor was any significant difference found between the number of Mormon and non-Mormon women who breast …

Growth And Uptake Dynamics Of Selenastrum Capricornutum Parameterized By Percent Nitrogen, Ronald F. Malone, Kenneth A. Voos, William J. Grenney

Reports

Batch cultures of

The Development And Validation Of A Life-Change Checklist For Juvenile Delinquents, Paul G. Kulcsar

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

A method generated by psychophysics has been used to develop a Checklist consisting of 58 life-events that require varying degrees of readjustment on the part of adolescents experiencing them.

A very high degree of concordance was found to exist among psychologists, social workers and probation officers with regard to the relative value of life-change required by those events.

Information about the occurrence of the amount of life-change was subsequently gathered from a sample of 334 juvenile delinquents and 104 nondelinquents by administering the Checklist. A measure of the degree of severity of delinquency was also obtained for each delinquent subject. …

Detection Of Neonatal Calf Diarrhea Virus (Ncdv) And Human Infant Reolike Diarrhea Virus, Martin Wyatt Peterson

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The purpose of this study was to develop a diagnostic test and conduct a survey for the neonatal calf diarrhea virus (NCDV) and human infant reolike diarrhea virus. Two immunologic methods were developed in this investigation.

Immune electron microscopy (IEM) and the fluorescent viral precipitin test (FVPT) are the methods used to detect NCDV and the human virus. Both methods are based upon the principle that viral aggregates form when the virus is reacted with anti-NCDV antibody. Aggregates in the IEM method are negatively stained and observed with the use of an electron microscope. Fluorescein labeled antibody is used in …

Monoamine Oxidase As A Target Enzyme In Pineal And Testicular Tissue: Adrenal Cortical Steroids And Arginine Vasotocin, Randall James Merrill

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The purpose of the research was twofold: (a) To ascertain what effects the adrenal glucocorticoids and catecholamines have on pineal N-acetyltransferase and monoamine oxidase activity, and (b) to observe what effect arginine vasotocin (a pineal polypeptide) has on testicular monoamine oxidase activity.

Rat pineal N-acetyltransferase activity was numerically increased when rats were injected with cortisol. It was increased significantly when the animals were injected with cortisol plus norepinephrine. Adrenal demedullation significantly increased N-acetyltransferase activity in starved rats when compared with intact control animals. Moreover, bovine pineal monoamine oxidase activity was markedly inhibited in a dose-dependent manner when incubated in vitro …

The Young Child's Concept Of Human Conception And Birth, Julie Ann Broberg Puzey

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The views held by children about the ere at ion and birth of babies are of importance in child development and developmental psychology. However, most of the research done in this area has been of a theoretical nature rather than empirical. The research shows controversy in the child's concept of human conception and birth, and because of this controversy a study was deemed necessary to find out more of what the young child's concept is of human conception and birth.

The purpose of this study, therefore, was to attempt to determine the extent of children's awareness of the nature of …