Digital Commons Network^™

In Vitro And In Vivo Replication For Scanning Electron Microscopy Of The Cervical Region Of Human Teeth, Joan Bevenius, Kjell Hultenby

Scanning Microscopy

A replica technique for scanning electron microscopy (SEM) of the cervical region of human teeth was evaluated on extracted premolar teeth by comparing the replicas and the original specimens in the SEM. For in vivo application, the technique was modified to circumvent contamination by saliva and gingival exudate. Impressions were taken with an addition silicone polyvinylsiloxane material and the replicas were poured in epoxy resin die material. A surface active dentine conditioner facilitated flow of the impression material into irregular surface areas; in vivo a scavenger impression was used to remove surface debris. Custom trays were made of light-cured acrylic …

Effect Of A Platinum Chemotherapy Drug On Intracellular Elements During The Cell Cycle, Using X-Ray Microanalysis, P. G. Edwards, M. D. Kendall, I. W. Morris

Scanning Microscopy

Intracellular elemental concentrations were measured in the cytoplasm, nucleus and nucleolus of cultured Chinese hamster ovary (CHO) cells, using energy dispersive electron probe X-ray microanalysis and transmission electron microscopy. Synchronous CHO cell populations were analyzed at different times during the growth cycle. The elements K, P, Mg and Zn were all more concentrated in the nucleus and nucleolus than in the cytoplasm, while no specific subcellular compartmentalization was evident for the elements Na, Cl, Ca, Fe and S. Significant changes in intracellular elemental concentrations were associated with the progression of cells from G₁ phase to S phase of the …

Beam Sensitivity Of Globoid Crystals Within Seed Protein Bodies And Commercially Prepared Phytates During X-Ray Microanalysis, Irene Ockenden, John N. A. Lott

Scanning Microscopy

Magnesium, potassium, calcium and phosphorus are stored in seed tissues in spherical particles called globoid crystals. The main component of globoid crystals is phytin, a salt of myo-inositol hexaphosphoric acid. Based on chemical similarity, commercially available phytates may be suitable standards for quantitative energy dispersive X-ray (EDX) microanalysis of globoid crystals. The stability of globoid crystals and commercial phytates was different when analyzed under identical conditions. Phytates in globoid crystals from Cucurbita maxima cotyledons, were relatively stable in the electron beam during EDX microanalysis at room temperature, but there was a loss of potassium. No loss of potassium occurred during …

The Vestibular Epithelia In Experimental Hydrops, K. C. Horner, S. Rydmarker

Scanning Microscopy

Morpho-pathological features, observed by scanning electron microscopy, in guinea pigs with endolymphatic hydrops of 4-14 months included shortening of the hair cell tufts, loss of tufts, retraction of sensory hair cells away from the surrounding tissue and hair cell loss. After 22 months of hydrops, there was complete loss of hair cells with indifferentiation of the epithelium. The loss of ciliary tufts involved loss of both stereocilia and kinocilia identified as short stubs and holes respectively. Control macular epithelia showed no hair tuft loss although ampullae in control ears could show some loss.

Morphological Study Of Calcospherites In Rat And Rabbit Incisor Dentin, H. Mishima, T. Sakae, Y. Kozawa

Scanning Microscopy

Calcospherites from incisors of rats and rabbits were studied, by means of secondary and backscattered electron images, using scanning electron microscopy. Part of each incisor specimen was made anorganic to allow observation of the surface of the mineralization front by scanning electron microscopy; the other part was ground on one side for observation by scanning electron microscopy with a backscattered electron image detector. In both species the labial mineralization front was wavy and the image showed fused calcospherites, whereas the lingual mineralization front appeared to consist of a combination of linear and globular forms. In rat labial dentin the calcospherites …

Utah Science Vol. 52 No. 2, Summer 1991

Utah Science

42 A Focus on Natural Resources Range Science Department

43 Determining How Rangeland Plants Get Along

44 Desert Crusts: Irreplaceable Veneer or Ecological Frosting?

47 Rhizomes vs. Tussocks: Which Grow Better?

48 Like Us, Plants will Notice Ozone Depletion

50 Grazing May Be Unfairly Blamed for Changes in Pristine Areas

51 Underground Events May Be Root of Grass Behavior Geography and Earth Resources Department

53 New Geography Simplifies Complexity Fisheries and Wildlife Department

55 Complexity, Human Expectations Alter Fisheries and Wildlife Research

56 Healthy Fish--Barometer of a Healthy Environment

59 USU Program Helps Manage Forests for Fish

60 Millions Flock …

Scanning Electron Microscopy Of Corrosion Casting In Medicine, M. A. Konerding

Scanning Microscopy

The aims of this review are: 1. to provide a bibliography of the publications that have used the corrosion casting technique; 2. to describe the advantages and limitations of the methodology; 3. to illustrate possible applications in the field of medicine, and 4. to highlight the significance of this method in the teaching of medical students. Thus, this paper is primarily focused on the scanning electron microscopical examination of vascular corrosion casts.

The unsurpassed three-dimensionality of the corrosion casting technique compared to any other means stands out in particular. This can be especially useful when complex vascular-anatomical relationships are present. …

Preparation Of Inner Ear Sensory Hair Bundles For High Resolution Scanning Electron Microscopy, M. P. Osborne, S. D. Comis

Scanning Microscopy

Chemical fixation techniques for preservation of sensory hair bundles in the mammalian inner ear for scanning electron microscopy (SEM) are reviewed. Fixatives employed were glutaraldehyde, glutaraldehyde-picrate, glutaraldehyde-tannic acid, glutaraldehyde-formaldehyde, glutaraldehyde followed by postfixation with osmium tetroxide and the osmium thiocarbohydrazide (OTOTO) method. Dehydration was routinely accomplished with ascending grades of acetone followed by critical point drying with liquid CO₂ or fluorocarbon sublimation. Specimens other than those prepared by the OTOTO method were metal coated with gold, gold-palladium or platinum. Material was viewed at high resolution (2-3 nm) in a transmission electron microscope (TEM) fitted with a scanning system and …

Mink Research: Wet Vs. Wet/Pellet Feeding Programs, Troy W. Hobbs

Undergraduate Honors Capstone Projects

Hobbs Fur Farm, of Franklin, Idaho, has been feeding the Fur-Pro pellet (made by Fur Breeders CO-OP, Midvale, Utah) for the past 8 years. Tim Hobbs, the owner, questioned the efficacy of the pellet feed. His concern was the quality of pelt being produced by mink on the wet/pellet feed program. A comparison of mink raised on the wet/pellet feed program vs. mink raised solely on a wet feed program was performed to determine if any significant differences could be noted.

Dichotemies Of The Human Perception Of The Environment, Janet K. Robson

Undergraduate Honors Capstone Projects

Environmentalism was not introduced in the year 1991, nor was it introduced with the era of the sixties. Perhaps, it is best to say that environmentalism has existed since the dawn of time--an environmentalism consisting of a "hands-off" policy. However, movements based on the environment have experienced an evolution in the past 30 years pertaining to the nature of preservation of wilderness areas and natural resources.

Elemental Mapping Of Cryosections From Cnidarian Nematocytes, Karl Zierold, Pierre Tardent, Sergey V. Buravkov

Scanning Microscopy

The distribution of elements in stinging capsules containing cells called nematocytes is shown by pseudocoloured maps representing the X-ray intensity collected from freeze-dried cryosections. This method provides a distinct overview in addition to the quantitative evaluation of single X-ray spectra. Selected examples illustrate the elemental compartmentation in various cnidarian animals. In particular the matrix of capsules in Hydra vulgaris contains high amounts of K in comparison to the tubule, the surrounding capsule wall and the cytoplasm, whereas in Actinia eguina capsules have either high concentration of Ca or Mg, the latter accompanied by S.

The Interaction Of Harvester Ants And Vesicular-Arbuscular Mycorrhizal Fungi In A Patchy Environment: The Effects Of Mound Structure On Fungal Dispersion And Establishment, Carl F. Friese

Biology

The major objective of this dissertation was to describe some of the factors that may be important in the establishment of vesicular-arbuscular (VA) mycorrhizal fungi following a disturbance. The specific focus of the dissertation is primarily on the interaction of the western harvester ant and VA mycorrhizal fungi and how this interaction affects the dispersion and establishment of these mutualistic endophytes. Methods were developed to test the use of polyclonal antibodies on field and greenhouse samples. This approach proved viable and allowed me to differentiate between both the extramatrical hyphae and spores of introduced and native VA mycorrhizal fungi. I …

Immunocytochemical And Histopathological Investigation Of The Hypothalamic-Pituitary-Testicular Axis Of The Primary Infertile Dark Mink (Mustela Vison), Baha M. Alak

Biology

This study was designed to investigate the hypothalamic-pituitary-testicular axis of the primary infertile dark male mink. The specific objectives were (1) to describe the morphology and localization of the neurons containing gonadotropin-releasing hormone (GnRH) and its precursor (proGnRH) within the preoptic-basal hypothalamus (POA-BH) of the sexually mature dark male mink, (2) to ascertain any differences in the number or the distribution of GnRH- and proGnRH-containing neurons between the fertile and infertile dark male mink, (3) to determine if GnRH and proGnRH are produced in insufficient amounts by the hypothalami of primary infertile dark male mink, and (4) to describe the …

Valuing Diversity In Business, Kimberly Nalder

Undergraduate Honors Capstone Projects

The purpose of this project was to create, plan, and implement an original instructional module on the topic of valuing diversity in business. My areas of emphasis were gender, sex role issues, and communication.

My responsibilities included: coordination with the instructor in all stages of planning and implementation; researching appropriate learning models for such a course; identifying topics of greatest significance within diversity; preparing and presenting one complete day of instruction; adapting cases and worksheets for both the course introduction and the gender issues/ communication sections; conducting personal interviews with appropriate resource individuals; creating an original group project; arranging for …

Spider Woman, Michelle Henrie

Undergraduate Honors Capstone Projects

This paper is an exercise in thinking and in writing, both of which I model after Elie Wiesel's Messengers of God: Biblical Portraits and Legends.

Creating Ethnicity In The Hydraulic Village Of The Mormon West, Charles M. Hatch

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

This study has looked behind the mask of nineteenth-century theocracy to see Mormons in the Great Basin creating a democratic society of regionally concentrated kin groups where obligations and rewards for individuals were increasingly determined by age and life cycle position. As generations of young adults acted together in self-interest dispersing their villages on receding frontiers, they forged a balance between competition and cooperation which merged the immediate need of individuals to establish and support families with the collective memory of their Mormon past. In so doing, they created an identity for themselves which was unique in the arid West. …

Ethical Beliefs And Practices: Do Psychologists Differ From Other Health-Care Professionals, Gary Percival

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Ethical codes and principles, and laws govern the behavior of health-care professionals. Yet, the impact that ethical codes and laws have on the actual moral behaviors of health-care professionals is relatively unknown. A survey on the ethical beliefs and practices of health-care professionals was sent to the United States membership of the Association for Applied Psychophysiology and Biofeedback. AAPB's heterogeneous membership offers a unique opportunity to compare the ethical beliefs and behaviors of professionals across various health-care professions, educational levels, licensure and certification statuses, age, years in practice, and gender. The survey examined the respondents' ethical beliefs and practices in …

Economic Strategies For White-Tailed Deer And Livestock Production On Central And South Texas Ranches, Will E. Cohen

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Recently, hunting and associated recreation have become an important business for Texas landowners. Most ranchers want to realize maximum net return from their investments. This study's objectives were to develop white-tailed deer (Odocoileus virginianus) and livestock enterprise budgets and to determine optimal economic combinations and levels of deer and livestock enterprises to maximize net returns. Questionnaires were mailed to landowners in the Edwards Plateau and South Texas Plains who purchased shooting preserve licenses in 1985. Five hundred and twenty-four ranchers responded (23.4%). Respondents were segregated by enterprises (deer, cattle, sheep, and Angora and Spanish goats) and a 5% …

The Play Of Visually Impaired Preschoolers With Their Mothers, Claudia Weber

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

This thesis answers the following questions: (a) Is the Play Assessment Scale a true measure of development? (b) Does the mother have a significant, positive influence on the child's level of development? (c) Does the mother's interactional style influence the child's development as measured by the Play Assessment Scale and the Battelle Developmental Inventory? The subjects were 13 visually impaired preschoolers. Development was measured with the Play Assessment Scale and the Battelle Developmental Inventory. Maternal interactional style was assessed with the Parent/Caregiver Involvement Scale. The study indicated that the Play Assessment Scale is a valid, reliable measure of development in …

Identification Of Potential Adhesins Shared Among Isolates Of Actinobacillus Species And Actinobacillus-Like Bacteria Cultured From Ram Lambs With Clinical Epididymitis, Yu-Wen Liu

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Ram lamb epididymitis, a serious reproductive disease of sheep, is caused principally by bacteria belonging to the genera Haemophilus and Actinobacillus. Six bacteria were studied: the American Type Culture Collection (ATCC) of Actinobacillus seminis (ATCC 15768), ATCC of Actinobacillus actinomycetemcomitans (ATCC 29522), field isolates of A seminis (86722 and 4101) and field isolates of Actin obacillus- like bacteria (Y136 and D107). The objectives of this study were to quantitate the adhesion of these 6 bacteria to bovine kidney epithelial cells (BKECs) and ram epididymal epithelial cells (REECs), evaluate the effect of rabbit polyclonal antibody prepared against ATCC 15768 …

Development And Evaluation Of An Expert System For Use As An Aid In Culling Dairy Cattle, Max L. Checketts

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

An expert system for identifying cows to be culled, MAXCULL, was programmed to run on an IBM or compatible personal computer. It was designed to be used with Dairy Herd Improvement (DHI) records as an aid in decision making. MAXCULL used fifty-two health, seventeen reproduction, and thirty-nine production rules in the analysis.

MAXCULL was initially developed using two expert system tools. VP-Expert and Super Expert both had inductive abilities and were reasonably priced. VP-Expert was selected to continue the development of the MAXCULL system. The program used a rule-based method of storing knowledge, which was obtained from literature reviewed in …

Evaluation Of Antiviral Compounds Against Avian Influenza, Evan W. Call

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Tests in vitro for antiviral activity against avian influenza viruses, A/Turkey/Sanpete/85 (H6N8) and A/Turkey/Sanpete/86 (H10N9), isolated in Sanpete County, Utah, utilized known antiviral agents, amantadine⋅HCl (adamantanamine hydrochloride) and ribavirin (1-β-D ribofuranosyl-1,2,4-triazole-3-carboxamide). The testing involved evaluation of seven drug concentrations. Maximum tolerated dose, minimum inhibitory concentration and therapeutic indexes were determined for each drug used. Both drugs demonstrated reasonable antiviral activity. Then they were tested against the H10N9 strain in 10-day-old Broad White turkey poults. Large- and small-particle aerosol delivery systems were the delivery methods utilized. Various parameters, including water and feed consumption, health score (based on visible signs), recoverable lung …

Factors Affecting Seeds In A Sagebrush-Steppe Ecosystem And Implications For The Dispersion Of An Annual Plant Species, Cheatgrass (Bromus Tectorum L.), Michael Ira Kelrick

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

I investigated how shrub-induced spatial heterogeneity influenced and was manifested by a representative ground-story plant species at a sagebrush-steppe site in southwestern Wyoming. The dispersion of cheatgrass (Bromus tectorum) reflected differences between undershrub (higher plant densities) and interspace (between shrubs, supporting lower densities) microenvironments, hence the population ecology of this annual species served as a biological probe of shrub-associated patch structure in this community. Since cheatgrass is an annual, factors affecting the seed portion of its life cycle were of special interest. First, attributes of the above- and below-ground seed pool were characterized. The environment-wide seed depositional pattern …

Reproductive Strategies Of Northern Goshawks And Cooper's Hawks In North-Central New Mexico, Patricia L. Kennedy

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

I assumed that the reproductive strategies of the two Accipiter species investigated in this study, the northern Goshawk (A. gentilis) and the Cooper's hawk (A. cooperii), would be similar to the strategies of other monogamous raptors with sex role partitioning. Any differences in the species' reproductive strategies, particularly hunting strategies, were expected to be a function of their size differences. Contrary to predictions, results indicated that, in north-central New Mexico during brood rearing, both species had similar hunting strategies and utilized similar prey species. These results also indicated that the reproductive strategies of the Cooper's hawk …

Study Of Ferredoxin And Flavodoxin From Tobacco Leaves And Roots Under Normal And Iron-Stressed Conditions, I-Jen Huang

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

I purified tobacco leaf ferredoxin from normal and iron-stressed leaves. The oxidized form of ferredoxin from normal leaves had absorption maxima at 268, 327, and 422 nm and a shoulder at 463 nm. The oxidized form of ferredoxin from iron-stressed leaves had absorption maxima at 268 and 329 nm but no absorption maxima in the 400-nm range. Higher ferredoxin content was found with higher chlorophyll content in leaves of the same age.

A protein in tobacco root extract was isolated and found to serve as an electron carrier for ferredoxin-requiring nitrite reduction. This protein had a molecular weight and antigenic …

Heterologous Expression And Characterization Of Three Active Recombinant Fungal Peroxidases From Phanerochaete Chrysosporium Using Baculovirus, Todd M. Johnson

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

In a collaborative effort with Dr. Steven D. Aust's group at Utah State University, this research is the first to describe the heterologous expression of active peroxidases from the filamentous fungus Panerochaete chrysosporium. The cDNA clones λML-1, λML-6, λML-5 and λMP-1, which had been tentatively identified as fungal peroxidase clones, were subcloned into baculovirus transfer vectors and recombinant Autographa californica nuclear polyhedrosis viruses were constructed which contained copies of these potential peroxidase genes. The recombinant baculovirus clones were used to infect insect cells for the heterologous production of active recombinant enzymes. Three recombinant lignin peroxidases (H2, H6 and H8) …

A Comparison Of Estimation Procedures For The Beta Distribution, Huey Yan

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The beta distribution may be used as a stochastic model for continuous proportions in many situations in applied statistics. This thesis was concerned with estimation of the parameters of the beta distribution in three different situations.

Three different estimation procedures-the method of moments, maximum likelihood, and a hybrid of these two methods, which we call the one-step improvement-were compared by computer simulation, for beta data and beta data contaminated by zeros and ones. We also evaluated maximum likelihood estimation in the context of censored data, and Newton's method as a numerical procedure for solving the likelihood equations …

The Swett Homestead: An Oral History 1909-1970, Eric G. Swedin

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Making extensive use of oral interviews with the surviving children, this thesis is an biography of Oscar and Emma Swett and their children, who lived on a homestead in Greendale, Utah, (near Flaming Gorge Reservoir) from 1909 to 1970. The family is representative of a group of families who moved to Greendale and engaged in small-scale cattle ranching. The introduction of new technology changed their lifestyles and homestead economics, while simultaneously Greendale evolved from a rural agricultural environment to become part of a National Recreation Area.

Hydrogen Uptake Genes And Nitrogen Fixation Efficiency Of Rhizobium Species In Symbiosis With Alfalfa, Chickpea And Pigeonpea, G. Mustafa Sajid

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The plasmids pDN211 and pDNll, isolated from the gene bank of the Rhizobium japonicum strain I-110, have been reported to complement two different Nif⁺ Hup^· (nitrogen fixation positive and hydrogen uptake negative) mutants. A 5.9-kb Hindiii DNA fragment of the cosmid pHU52, isolated from the gene bank of R. japonicum strain 122DES, has been reported to code for the two polypeptide subunits of uptake hydrogenase. To determine homology between the structural genes of uptake hydrogenase of the two strains, a Southern blot of the Hindiii restriction fragments of the plasmids pDN211 and pDN11 was hybridized to the 5.9-kb …

Potassium And Sodium Interrelations In Alfalfa Phenotypes Grown On Calcareous Soil, Suresh S. Dhumal

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Three greenhouse experiments were conducted with three phenotypes of alfalfa (Medicago sativa L.) obtained from a potassium (K)-deficient field and with their diallel crosses grown on low K soil. The first experiment was conducted to study the partitioning and broad-sense heritability of K and sodium (Na) between leaves and stems of the three phenotypes which were deficient in K and exhibited normal (N), marginal chlorotic (M), and white spot chlorotic (W) leaflets. The second experiment was conducted to study the partitioning of K and Na in leaves, stems, and roots as influenced by 32 alfalfa crosses obtained from diallel …