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Cochlear And Vestibular Epithelia From A Patient With Meniere's Disease: A Case Study, K. C. Horner

Scanning Microscopy

Scanning electron microscopy observations were carried out on the cochlear and vestibular epithelia of the left temporal bone of a Meniere' s patient. There was almost complete absence of hair cells in the basal turn of the cochlea. The outer hair cells of the second turn presented an abnormal shortening of the shorter stereocilia within a tuft, reminiscent of the specific atrophy of the short and middle stereocilia in the ciliary tufts of outer hair cells in the guinea pig with experimental hydrops. The cilia of the inner hair cells showed fusion and giant cilia formation. Hair cells were observed …

The Splenic Microvasculature Of The Red-Eared Turtle (Chrysemys Scripta Elegans): A Study Concerning The Question Whether The Circulation Is Anatomically Open Or Closed, R. Blumer, H. Ditrich, H. Splechtna

Scanning Microscopy

The splenic microvasculature of the turtle Chrysemys scripta elegans was studied by means of scanning electron microscopy of vascular corrosion casts and critical point dried tissue. In addition light- and transmission electron microscopic investigations were carried out. Within the organ the arteries are surrounded by periarterial lymphoid sheaths. The majority of the arterial capillaries, which emerge from these central arteries have open endings within the reticular meshwork of the red pulp. Approximately 10% of the capillaries directly connect with the venous origins. Since these venous capillaries of C. scripta elegans resemble those of nonsinusal mammalian spleens we termed them pulp …

Response Of The Esophageal Epithelium To Concomitant Cis-Dichlorodiammineplatinum(Ii) And Radiation Treatment. An Electron Microscopic Study In Rabbits, M. Albertsson, M. Cwikiel, C. H. Håkansson, M. Palmegren

Scanning Microscopy

The rabbit esophageal mucosa was irradiated with daily fractions of 2 Gy up to an accumulated dose of 20 Gy (total dose 2, 6, 10. 16 or 20 Gy). Fifteen to forty-five minutes before the start of each irradiation 0.3 mg Cis-dichlorodiammineplatinum (cis-DDP, cisplatinum) was given by intraperitoneal injection to each rabbit. Examinations were carried out 1-10 days after each fractionation schedule, when specimens were taken for morphological investigations.

Scanning electron microscope (SEM) examination showed a gradual development of damage with cell loss and structural disarrangement of the microridges and whorls on the surface. However, with further treatment the esophageal …

Can Antibiotic Test Kits Be Useful?, Bart Weimer

Archived Food and Health Publications

Publication discusses whether or not antibiotic test kits are useful and what the various results mean.

Utah Science Vol. 53 No. 3, Fall 1992

Utah Science

68 Native vs. Introduced Species: The New Range War

79 Wilderness Use Difficult to Predict

83 Research in Brief

87 Globemallows Ready for Release

89 Grazing Effects: There' More to Learn

92 USU Study Examines Costs, Benefit of Wilderness Designation

98 Revegetating Rangelands: Small Phenomena Make a Big Difference

The Effect Of Varying Environmental Conditions On Phytolith Morphometries In Two Species Of Grass (Bouteloua Curtipendula And Panicum Virgatum), T. B. Ball, J. D. Brotherson

Scanning Microscopy

Solid deposits of SiO₂ (phytoliths) accumulate in many plants in specific intracellular and extracellular locations. Phytoliths have morphological characteristics unique to some taxa and therefore have taxonomic significance. Phytoliths persist and maintain their morphological integrity long after a plant has died, thus becoming a microfossil of the plant that produced them. Development of phytolith sytematics for microfossil phytoliths has traditionally followed a typological approach based on simple verbal descriptions of shape. A new method for use in phytolith sytematics is the morphometric approach which employs computer-based Image Analysis Systems to make quantified measurements of morphological parameters (size, shape, texture, …

Scanning Electron Microscopy Of Nerve Fibers In The Dog Cochlea, Hirofumi Morita, Tomoyuki Hoshino, Kunihiro Mizuta

Scanning Microscopy

The nerve fiber arrangement inside the organ of Corti in the dog was studied. Thick sections were cut serially from celloidin-embedded cochleas and observed with a scanning electron microscope (SEM). The nerve fibers in the organ of Corti were clearly exposed in sections cut in the horizontal plane. The arrangement of nerve fibers in the dog showed considerable difference from that of other species. The tunnel basilar fibers in the dog curved basalward, and took a long longitudinal course to form a broad bundle in the center of the tunnel floor. This bundle has not been found in adult animals …

Microwear Studies Of Early African Hominid Teeth, Pierre-François Puech

Scanning Microscopy

Studies of microwear on fossil hominid teeth have become an important approach in paleoanthropology. Since there are very few samples of early hominids from East Africa with suitable teeth, this paper presents a review of the occurrence of some dental wear characteristics to provide a data base for reconstructing the diet of the early hominids.

Distinctive microwear features such as furrows, crenulations, stress lines and deep grooves, are interpretive tools that can be used in a biomechanical approach. Occurrence of the same dental microwear patterns in extant species with known dietary differences is indicative of foods that have probably been …

Comparison Of Cryopreparation Techniques For Electron Probe Microanalysis Of Cells As Exemplified By Human Erythrocytes, Karl Zierold

Scanning Microscopy

Erythrocytes in human blood were used to evaluate the reliability of cryopreparation techniques for electron probe X-ray microanalysis of biological cells and tissues. The elemental content determined by X-ray microanalysis of ultrathin freeze-dried cryosections was found to be consistent with data known from the literature. Considerable redistribution of the intracellular elemental composition was found after freeze-substitution as well as after freeze-drying followed by resin embedding. Two conclusions are drawn from this study: 1. Erythrocytes in human blood are a suitable reference specimen for evaluation of specimen preparation techniques for microanalysis. 2. At present, freeze-dried cryosections are the most reliable specimen …

Information And Misinformation Regarding Ischemia Of Heart Muscle Tissue. The Cause Of Cell Death During Blood Reperfusion And Reactivation Of Heart Muscle Tissue After Prolonged Ischemia, Fritiof S. Sjöstrand

Scanning Microscopy

An electron microscopic study of heart muscle tissue exposed to six hours ischemia and prepared according to the low denaturation embedding technique revealed a structural modification confined to the mitochondrial cristae. The modification consisted of a removal of Krebs cycle enzymes from the cristae. Reperfusion of the ischemic tissue after four hours ischemia led to extensive breakdown of the mitochondrial structure and contractility could not be restored. However, when after six hours ischemia the ischemic tissue was reperfused with blood, the composition of which had been modified to stimulate mitochondrial function, no additional structural changes were observed and contractility was …

Morphological Study Of Gastric Lesions Developing In The Rat Under Several Damaging Conditions: Modifications Induced By Pretreatment With Zinc Acexamate, M. Luisa Bravo, Ginés Escolar, Carmen Navarro, Ramón Fontarnau, Oriol Bulbena

Scanning Microscopy

Lesions developing in the gastric mucosa of the rat after exposure to different gastric damaging agents (100 mg/kg aspirin, and 70% or 100% ethanol) were assessed by scanning electron microscopy. The severity of the lesions was quantified according to morphological criteria. Modifications in the severity of these lesions induced by pretreatment with zinc acexamate were also analyzed. The scanning electron microscope revealed that with the exception of absolute ethanol, which caused distinctive morphological features, lesions found under the different experimental agents shared a common pattern of progression. Ultrastructural lesions on surface epithelial cells preceded further alterations of parietal cells. After …

Electron Probe Microanalysis Of The Otolithic Membrane. A Methodological And Quantitative Study, J. A. López-Escámez, F. J. Cañizares, P. V. Crespo, A. Campos

Scanning Microscopy

The effect of tissue preparation on calcium and potassium weight percents in the otoconial layer in the utricle and saccule was studied in four groups of OF1 mice with electron probe X-ray microanalysis. Glutaraldehyde and freeze-drying, glutaraldehyde and air-drying, air-drying, and cryo-fixation and freeze-drying were compared. Ca and K changed significantly in the utricle depending on the method used (P < 0.001), and K changed significantly in the saccule (P < 0.001 ). We chose cryo-fixation with freeze-drying for the quantitative analysis of the otolithic membrane because this method provided the highest values of Ca and K with minimum loss of Ca and K . Microcrystalline salt standards mounted on scanning electron microscopy holders were used for the quantification of Ca and K by the peak-to-local-background (P/B) ratio method. The P/B ratio in standards with reproducible results, when plotted against weight percent, gave a straight line for Ca (r = 0.99, P < 0.001) and K (r = 0.98, P < 0.001). The Ca and K weight percents in otoconia showed similar frequency distributions in the utricle and saccule.

Arthropathies Associated With Basic Calcium Phosphate Crystals, Paul B. Halverson

Scanning Microscopy

Basic calcium phosphate (BCP) crystals refer to a family of crystals including partially carbonate substituted hydroxyapatite, octacalcium phosphate, and tricalcium phosphate. These crystals have been found in and around joints and have been associated with several forms of arthritis and periarthritis. Identification of BCP crystals remains problematic because of the lack of a simple, reliable analytic procedure. Methods currently in use include alizarin red S staining, labelled diphosphonate binding, scanning and transmission electron microscopy with energy dispersive X-ray microanalysis, X-ray diffraction, and atomic force microscopy. Periarthropathies associated with BCP crystals include calcific tendinitis and bursitis. Intra-articular BCP crystal deposition is …

Selection And Development Of Replacement Beef Heifers, Norris J. Stenquist, James A. Bennett

Archived Agriculture Publications

Selection, development, management, and nutrition of heifer calves to be used as herd replacements are key factors in maintaining beef herd productivity. A cow/calf producer, to be successful, must have some goals or criterion for heifer selection and development. For practically all Utah cattle production areas, economics dictate that heifers must calve as two-year-olds. Heifers must be properly developed and managed to permit this. The following factors are important: Heifers should 1) become pregnant in the first 25 days of the breeding season, 2) give birth to a live calf with little or no calving difficulty, 3) raise a calf …

Photosynthesis And Leaf Morphology Of Liquidambar Styraciflua L. Under Variable Urban Radiant Energy Conditions, Roger Kjelgren, James R. Clark

Plants, Soils, and Climate Faculty Publications

Diminished sunlight, characteristic of urban canyons, has been suggested as being potentially limiting to plant growth. This study investigated the response of sweetgum (Liquidambar styraciflua L.) to variable irradiance in a range of urban locations. Diurnal photosynthesis was measured in situ on mature trees, comparing an open site at an urban park with an urban canyon that received 4 h of midday sun in midsummer. Photosynthesis for trees growing in the canyon was lower both during shaded and sunlit periods compared with trees at the park. Photosynthesis of detached shoots in a growth chamber was greater in canyon than park …

Luminal Constrictions On Corrosion Casts Of Capillaries And Postcapillary Venules In Rat Exocrine Pancreas Correspond To Pericyte Processes, Seyedhossein Aharinejad, Peter Böck

Scanning Microscopy

The rat exocrine pancreas was studied as a model to demonstrate morphological features of different types of capillaries, using scanning electron microscopy of vascular corrosion casts and transmission electron microscopy of tissue sections. Two types of capillaries were discerned. The first type represents less undulated, slender, straight capillaries with numerous, shallow, circular or semilunar furrows on its cast's surface. In tissue sections, this type probably corresponds to non-fenestrated capillaries. The numerous grooves on its cast correspond to pericyte processes beneath the endothelial lining. The second type comprises capillaries of an undulated course and variable diameter with less numerous furrows. In …

Appearance Of Venous Sphincters In The Pulmonary Microvascular Bed Of Normotensive And Spontaneously Hypertensive Rats, Seyedhossein Aharinejad, Peter Böck

Scanning Microscopy

The appearance of pulmonary venous sphincters was studied in normotensive and spontaneously hypertensive rats using scanning electron microscopy of microvascular corrosion casts and transmission electron microscopy of tissue sections. Vascular casts were prepared either after lavage with Tyrode solution or after glutaraldehyde prefixation. Pronounced pulmonary venous sphincters were more frequently identified in spontaneously hypertensive rats as compared to corresponding circular indentations in normotensive rats. Tissue sections established venous sphincters in hypertensive animals as consisting of multiple layers of smooth muscle cells in the venous walls. We did not observe any autonomic nerve terminals in close proximity to these bundles of …

Histochemical Demonstration And Microanalysis Of Possible Calcium Binding Sites In The Enamel Organ Of Rat Incisors, Y. Takano

Scanning Microscopy

The rat incisors obtained from rats perfused with high-calcium solution containing 30 to 50 mM CaCl₂ were processed for rapid freeze/freeze-substitution and embedded in epoxy resin. GBHA staining, a histochemical staining for calcium, of unhydrously prepared sections revealed a large number of granular Ca-GBHA reactions in the enamel organ, most of which being located along the lateral plasma membranes of the ameloblasts. In the ameloblast layer, the reaction was negative in the presecretory stage, became intense in concert with the onset of enamel matrix formation, and remained so by the end of the transitional stage where the reaction gradually …

Functional And Morphological Studies Of Mitochondria Exposed To Undecagold Clusters: Biologic Surfaces Labeling With Gold Clusters, E. Valdivia, C. Gabel, J. E. Reardon, C. A. Cajacob, H. Yang, R. S. Wehbi, G. L. Scott, P. A. Frey, L. A. Fahien

Scanning Microscopy

This study reports morphological and functional alterations observed in respiring isolated mitochondria when they are exposed to nonpenetrating, positive electrostatically charged synthetic undecagold clusters. Modification of the undecagold clusters positive charges change or prevent the functional effects and the binding to the outside surface of the mitochondria. The mitochondrial functional alterations are dependent on the oxidative phosphorylation capacity of the isolated organelles. The results of these experiments indicate that artificial undecagold may be useful to explore the molecular mechanisms of biological energy transducers which require electric charges separation, ionic fluxes, and electric surface properties.

Scanning X-Ray Microanalysis Of Microcarrier Cultured Endothelial Cells: Elemental Changes During The Transition To Confluency And The Effect Of Ionophore A23187, S. R. Hall, D. C. Sigee, J. E. Beesley

Scanning Microscopy

Porcine endothelial cells were grown on microcarrier beads and examined by scanning electron microscopy (SEM) at various times after initiation of culture. Total cell coverage on the bead surface varied from mean values of approximately 7% (3h) to 80% (96h). Beam penetration into the subcellular matrix presents a major problem with SEM X-ray microanalysis of microcarrier cultured cells and necessitates the use of an accelerating voltage not exceeding 10kV. At this voltage and below, X-ray contribution from elements present in the microcarrier bead has minimal effect on the determination of cell elemental levels. Washing the cells with 0.15M sucrose was …

Utah Science Vol. 53 No. 2, Summer 1992

Utah Science

34 Alfalfa: Still a Regal Crop

39 Good Seed A Prerequisite for Good Alfalfa

40 Eructate? Tympany? Hint: Both In lye Alfalfa and Air

44 Drought-Resistant Alfalfa: Maybe Only Relatives from the Gobi Desert Can Help

47 Research in Brief

51 Nematodes: Where They're At is Less Important

53 Than What You Do

56 Alfalfa May Find Wider Use on Rangelands Hay Quality: Opinions Still Count

60 N, P and K: It's Not A Simple As It Seems

64 Improving Nitrogen-Fixing Bacteria

Mechanisms Of Ciliary Movement: Contributions From Electron Microscopy, Peter Satir

Scanning Microscopy

A brief review of important contributions of electron microscopy to the study of ciliary motility is presented. The electron microscope was used to show the universality of axonemal structure of cilia, and to develop the sliding microtubule model of ciliary motility and later the switch point hypothesis to explain the conversion of sliding into bending. Unexpectedly, insights into the importance of cilia in human health have stemmed from these studies.

Microwear And Striae Of Retzius Of Canines Of The Wild Japanese Monkeys (Macaca Fuscata), Teruyuki Hojo

Scanning Microscopy

This is a description of microwear and striae of Retzius of canine teeth of wild-caught female Japanese monkeys (Macaca fuscata) from Oita Takasakiyama, Kyushu. Micrographs were taken of high resolution casts using a scanning electron microscope (SEM). Before the casts were made, the diet of the captive monkeys was water-softened for three or four days. This softened diet probably added little wear to the surfaces of the teeth. These animals are part of a sample used in a previous report published in this journal. Others have observed that wild Japanese monkeys eat hard foods (such as grasshoppers, cicads …

Early Effects On The Morphology Of Mouse Small Intestine Of Single Or Combined Modality Treatment With Hyperthermia And X-Irradiation, G. R. Dickson, H. M. H. Kamel, S. P. Hume, M. Jaber, K. E. Carr

Scanning Microscopy

This study describes the effects of hyperthermia and X-irradiation on the morphological appearance of normal, at risk tissues in the ileum of the mouse. The early morphological effects day after a combined modality treatment are compared with those due to either hyperthermia or X-irradiation given alone. The response was assessed qualitatively and semiquantitatively using scanning electron microscopy and a villous scoring technique.

Early post-irradiation effects on topography did not differ significantly from those observed after small intestine exteriorisation without treatment. The villous scores for the combined modality treatments reflected greater damage than would be expected from the sum of villous …

Utah State University Commencement, 1992 – Main Campus, Utah State University

Commencement Programs

Ninety-Ninth Annual Commencement of Utah State University

Three-Dimensional Characterization Of Dense Bodies In Contracted And Relaxed Mesenteric Artery Smooth Muscle Cells, Linda J. Mcguffee, Sally A. Little

Scanning Microscopy

We have previously shown that dense bodies are not the static planar simple ovoidal structures they appear to be in thin sections. In this report, we present three-dimensional reconstructions from consecutive serial thin sections through shortened and non-shortened large mesenteric artery cells. Profiles of the cell surface, membrane dense bodies, and cytoplasmic dense bodies were reconstructed from consecutive thin sections and the distribution, size, shape, and spatial relationships among these components was examined. Within the cell, membrane dense bodies are numerous and occupy approximately 10% of the cell volume. Membrane dense bodies can attach to the cell surface laterally, obliquely …

Scanning Electron Microscopy Of The Mammalian Organ Of Corti: Assessment Of Preparative Procedures, A. Forge, G. Nevill, G. Zajic, A. Wright

Scanning Microscopy

Different fixation, drying and coating procedures have been applied in preparation of the organ of Corti for scanning electron microscopy (SEM), and structural features of the apical surface of the tissue in unfixed, freeze-fractured preparations used in assessing their effects on morphology. Fixation with glutaraldehyde alone or osmium tetroxide alone causes artefacts that are substantially avoided when tissue is doubly fixed in glutaraldehyde followed by osmium. Significant improvements in preservation are also obtained when tissue is additionally processed through thiocarbohydrazide-osmium (TOTO) processing. In addition to providing a conducting coat, it stabilises the tissue against deformations that might otherwise occur during …

Final Environmental Impact Statement For Rangeland Ecosystem Management On He Uinta National Forest, Provo, Utah, Usda Forest Service

All U.S. Government Documents (Utah Regional Depository)

This Final Environmental Impact Statement describes alternatives, including a "No Action" alternative for management of National Forest Rangeland Resources on the Uinta National Forest. Alternatives range from no change from past management practices, which in some instances have resulted in less than favorable ecological conditions on National Forest Rangelands and riparian resources, to managing these resources to achieve the "Potential Natural Community" in terms of vegetative cover types and condition. The environmental consequences of all alternatives considered in detail are displayed. The alternatives selected for implementation will become an amendment to the Uinta National Forest Land and Resource Management Plan. …

Scanning Electron Microscopic Studies Of The Oral Mucosa And Its Microvasculature: A Review Of The Palatine Mucosa And Its Microvascular Architecture In Mammals, Y. Ohta, S. Okada, I. Toda, H. Ike

Scanning Microscopy

The present paper deals with the microvascular architecture of the palatine mucosa in primates, camivorae, and rodentia utilizing microvascular corrosion castings and epithelium-separated specimens.

The submucous vascular network is under-developed since the bard palatine mucosa was designated the mucoperiosteum, except some areas. The palatine venous plexus appears to show regional differences with animal species differences. The well-developed plexus is observed to be two-layered and may contribute to the process of regurgitation of rough food and assist in mastication with the palatine plicae. Formation and patterns of the arterial network in the lamina propria are in a close relation with connective …

The Use Of Organ Cultures To Study Vessel Wall Pathobiology, Edward W. Y. Koo, Avrum I. Gotlieb

Scanning Microscopy

Organ culture of the vessel wall is an useful in vitro method to study vascular cell biology. The intact vessel allows for the study of cell-cell and cell-substratum interactions including the structure and function of the vessel wall matrix. Long term organ cultures of porcine aorta show that neointimal formation is due primarily to cell proliferation of pre-existing intimal smooth muscle cells. Neointimal formation in these cultures is more pronounced in the presence of an endothelium that is turning over. In endothelial wound repair studies, the endothelium of the organ culture shows some important differences when compared to tissue culture …