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Art Historical Research Of The Intercession Of Christ And The Virgin, Attributed To Lorenzo Monaco, Lauren Watts May 2025

Art Historical Research Of The Intercession Of Christ And The Virgin, Attributed To Lorenzo Monaco, Lauren Watts

Curiosity

This research examines The Intercession of Christ and the Virgin, a painting attributed to Lorenzo Monaco. The painting is tempera on canvas, and is housed at the Metropolitan Museum of Art. This research looks at the painting through a phenomenological lens in context of its original site, iconographic themes, and feminine spirituality. The research posits that the painting’s intercessory figures—Christ and the Virgin Mary—construct a narrative amplifying themes of feminine spirituality, challenging the misogynistic structures of quattrocento Florentine ecclesiastical culture while remaining within the bounds of contemporary orthodoxy. Studying the painting at Metropolitan Museum facilitated in-depth visual analysis, supplemented by …


“Gang, We Have A Mystery On Our Hands!”: A Folkloristic Approach To Conspiracy Thinking In Scooby-Doo! Mystery Incorporated, Sydney K. Brooks May 2025

“Gang, We Have A Mystery On Our Hands!”: A Folkloristic Approach To Conspiracy Thinking In Scooby-Doo! Mystery Incorporated, Sydney K. Brooks

All Graduate Reports and Creative Projects, Fall 2023 to Present

In this thesis I examined how the 2010 children’s television show Scooby-Doo! Mystery Incorporated both utilizes and critiques conspiracy, embedded within its mystery-solving structure. Through a close analysis of the members of the gang—Fred, Daphne, Velma, Shaggy, and Scooby-Doo—I broke down their individual behaviors, interactions, and contributions to conspiratorial belief. I then transitioned into a case study of the episode “Where Stalks the Scarebear,” showcasing its connection to larger real-world conspiracy narratives, particularly those related to corporate and environmental cover-ups. Finally, I examined the show as a complete narrative, reflecting on its complex, serial exploration of conspiracy through the framework …


Selected Projects: Set Design And Projection Design, Audrey Kearl Coleman May 2025

Selected Projects: Set Design And Projection Design, Audrey Kearl Coleman

All Graduate Reports and Creative Projects, Fall 2023 to Present

Theatrical scenic and projection design for the stage.


Optimizing Nutrient Recycling To Achieve Zero–Discharge Management For Controlled Environment Agriculture, Noah James Langenfeld May 2025

Optimizing Nutrient Recycling To Achieve Zero–Discharge Management For Controlled Environment Agriculture, Noah James Langenfeld

All Graduate Theses and Dissertations, Fall 2023 to Present

Controlled environment agriculture (CEA) allows for human control of one or more environmental factors affecting crop production. Optimizing nutrient management in such systems allows for translation and predictions of sustainable management strategies in the field. These systems will be used to supply fresh food as public and private space agencies are planning to send humans to the Moon and Mars in the near future. Food resupply becomes increasingly difficult as the distance from Earth increases. Plants can be grown off-Earth as a food source, but their growth must be carefully managed to ensure long-term sustainability. I describe a model used …


Effects Of Plant Biostimulants On Onion Growth And Production Under Greenhouse And Field Conditions, Prakriti Nepal May 2025

Effects Of Plant Biostimulants On Onion Growth And Production Under Greenhouse And Field Conditions, Prakriti Nepal

All Graduate Theses and Dissertations, Fall 2023 to Present

The escalating impact of climate change on agricultural production underscores the need to investigate sustainable crop enhancement methods. This research explores the use of plant biostimulants to grow onion (Allium cepa L.) under varying environmental conditions. In the initial germination and greenhouse screening, seven biostimulants were tested on ‘Vaquero’ onion seeds: three bacterial products (Continuum, Spectrum DS, and Tribus Original), two mycorrhizal products (Mighty Mycorrhizae and Myco Apply), one seaweed extract product (Seaweed), and one product containing humic acid (Huma Pro 16). Mighty Mycorrhizae, Continuum, and Myco Apply supported faster germination, while seeds treated with Spectrum and Seaweed took …


High-Throughput Phenotyping Of Early Generation Small Grains Breeding Lines To Indirectly Select For Grain Yield, Dalton Jones May 2025

High-Throughput Phenotyping Of Early Generation Small Grains Breeding Lines To Indirectly Select For Grain Yield, Dalton Jones

All Graduate Theses and Dissertations, Fall 2023 to Present

In small grains breeding programs, breeders aim to develop better varieties by selecting for important traits like grain yield. However, it takes many years before yield can be measured because several generations of selection are required to fix lines and increase sufficient seed for yield measurements. This process can be slow and expensive

High-throughput phenotyping (HTP) is a modern approach that can increase breeding efficiency by leveraging technology to quickly measure secondary traits which can be used to indirectly select for important traits in crop plants. For example, researchers can use cameras on unmanned aerial vehicles (UAVs, i.e., drones) to …


A Model Of Changeover Behavior In Concurrent Schedules Of Reinforcement, Matias A. Avellaneda May 2025

A Model Of Changeover Behavior In Concurrent Schedules Of Reinforcement, Matias A. Avellaneda

All Graduate Theses and Dissertations, Fall 2023 to Present

Animals in the wild have access to a variety of food sources, and they must allocate their time between them in an efficient manner. This requires deciding when to depart from the source currently being exploited to travel to a different one. A general model of this decision process can be derived from three assumptions well supported by the learning literature. The first one is the so-called matching law, which says that the proportion of time allocated to any one behavior equals the proportion of total value obtained from that option. The second one is that the dwell times (i.e., …


Analyzing Microalgae-Bacteria Population Changes In Response To Environmental Parameters For Improved Wastewater Treatment, Amanda M. Moravek May 2025

Analyzing Microalgae-Bacteria Population Changes In Response To Environmental Parameters For Improved Wastewater Treatment, Amanda M. Moravek

All Graduate Theses and Dissertations, Fall 2023 to Present

Microalgae are incredible organisms that have a variety of uses such as wastewater treatment, fertilizer, livestock feed, and bio-compound production. Microalgae require a light source and minimal nutrients space to proliferate, making them an environmentally friendly and sustainable option for such processes. The Sustainable Wastes-to-Bioproducts Engineering Center at Utah State University is developing and implementing a Rotating Algae Biofilm Reactor (RABR) to remove total nitrogen and phosphorous from the Central Valley Wastewater Reclamation Facility wastewater effluent. Harmful compounds such as nitrates and phosphates are metabolized by the microalgae and converted into useful bioproducts, resulting in a circular economy. These RABRs …


Seeding Rate, Planting Date, And Herbicides For Dormant Seeded Safflower In Utah, Rodney Daniel Nelson May 2025

Seeding Rate, Planting Date, And Herbicides For Dormant Seeded Safflower In Utah, Rodney Daniel Nelson

All Graduate Theses and Dissertations, Fall 2023 to Present

Dryland crop production in the Intermountain West is largely limited by the amount of precipitation received. Increasing dryland safflower (Carthamus tinctorius L.) production yields in a semi-arid climate is possible with a dormant planting but is not well understood. A two-year (2020-22) project was designed to gain an understanding of the effects of variety, seeding rate, and planting date, on dormant seeded safflower. Chemical weed control options for safflower were also evaluated. One study, conducted at three Utah State University research farms near Blue Creek, Clarkston, and Kaysville, UT, evaluated four varieties, S-208, S-333, 7325 and Hybrid-446, seeded at …


Potamogeton Crispus Invasion: Impacts On Native Aquatic Plants And Associated Faunal Habitat, Meghan-Grace Slocombe May 2025

Potamogeton Crispus Invasion: Impacts On Native Aquatic Plants And Associated Faunal Habitat, Meghan-Grace Slocombe

All Graduate Theses and Dissertations, Fall 2023 to Present

Aquatic invasive plants are non-native plants that invade a water body and displace native aquatic plants. Invasion depletes the native plant community's ability to provide ecosystem services, including improved water quality, decreased problematic algae, and increased animal habitat. The loss of these ecosystem services reduces the value of fishing, boating, and swimming opportunities in aquatic ecosystems. The potential changes in plant communities and services are particularly important at sites targeted for restoration because project goals typically include increasing ecosystem services, including wildlife habitat. Curly-leaf pondweed (Potamogeton crispus L.) is a problematic invasive species in North America. To better manage …


Leadership, Identity, And Student Voice In Secondary Schools, Gordon S. Geddes May 2025

Leadership, Identity, And Student Voice In Secondary Schools, Gordon S. Geddes

All Graduate Theses and Dissertations, Fall 2023 to Present

Many student leadership programs in U.S. high schools operate without clear guidance, often leaving students as passive participants in systems that may not effectively prepare them for leadership roles both in school settings and elsewhere. This study explores how listening to students’ voices can improve leadership programs by better understanding how young people see themselves as leaders. Using a case study approach at one school, I observed student leaders over one school year and analyzed their experiences through leadership development theories. The findings suggest that when schools take students' perspectives into account, they can create leadership programs that are more …


The Effect Of Dietary Protein Source On Gut Health, Spencer Lawson Schow May 2025

The Effect Of Dietary Protein Source On Gut Health, Spencer Lawson Schow

All Graduate Theses and Dissertations, Fall 2023 to Present

We investigated the impact of protein source in a western-style diet on colon cancer, colitis, and the gut microbiome. The Western dietary pattern is energy-dense, low in fiber, and has a sub-optimal micronutrient profile. Our group has formulated the Total Western Diet (TWD) that emulates the Western dietary pattern using survey data (NHANES), and we have demonstrated that this diet increases gut inflammation and colorectal cancer relative to a healthy control diet (AIN-93G), which is optimized for mouse health. However, the effect of protein source in the TWD and its effects on gut health have yet to be investigated.

Recent …


Managing For Disturbance: Trade-Offs Associated With Population Resilience And Ecosystem Size, Ellie Wallace May 2025

Managing For Disturbance: Trade-Offs Associated With Population Resilience And Ecosystem Size, Ellie Wallace

All Graduate Theses and Dissertations, Fall 2023 to Present

Populations can recover from and withstand disturbance through characteristics such as reproduction and maintaining large abundances. While these traits are beneficial to native species conservation, they can simultaneously challenge effective invasive species management. Effective invasive species control requires sufficient efforts to overcome a population's ability to recover from disturbance, such as harvest. My dissertation aimed to address how the size of an invasive population and its ability to recover from disturbance impact a suite of invasive species control methods, including those that (1) target mainly larger and older individuals (i.e., mechanical removal), (2) target all sizes of individuals (i.e., targeted …


Uv-A Light And Heat-Activated Antimicrobial And Biodegradable Materials For Food Packaging And Preservation, Michael J. Bodily May 2025

Uv-A Light And Heat-Activated Antimicrobial And Biodegradable Materials For Food Packaging And Preservation, Michael J. Bodily

All Graduate Theses and Dissertations, Fall 2023 to Present

Current food packaging materials are often non-biodegradable and harmful for ingestion, which poses a threat to the environment and public health. Thus far, the dangers of microbial contamination of food on human health have outweighed the risks associated with using these conventional packaging plastics. A possible solution to these issues is to use a combination of innovative materials and microbial inactivation methods that can potentially both reduce plastic waste and combat harmful microorganisms. In this study, two antimicrobial, biodegradable composites were developed and tested for their ability to inactivate two types of bacterium, Escherichia coli K12 and Listeria innocua, …


Greater Sage-Grouse Habitat Dynamics And Grazing, A Study Of Vegetation Indices And Survival, Codi R. Backen May 2025

Greater Sage-Grouse Habitat Dynamics And Grazing, A Study Of Vegetation Indices And Survival, Codi R. Backen

All Graduate Theses and Dissertations, Fall 2023 to Present

North American rangelands are vast, open landscapes that support diverse ecosystems and provide many ecosystem services. Many wildlife call sagebrush rangelands home, including the Greater sage-grouse (sage-grouse; Centrocercus urophasianus). Sage-grouse habitat encompasses 165 million acres across western North America, and this bird has been of conservation concern as their populations drastically declined since the 1960s. Rangelands are also grazed by livestock, which has the potential to negatively affect sagebrush ecosystems when overgrazed or overstocked, which could be detrimental to sage-grouse nesting habitat. Additionally, sagebrush rangelands undergo annual variations in precipitation, temperature, growing season length, and vegetation vigor, which has …


Disentangling Factors Contributing To Population Performance Of Zion Bighorn Sheep, Ian Montgomery May 2025

Disentangling Factors Contributing To Population Performance Of Zion Bighorn Sheep, Ian Montgomery

All Graduate Theses and Dissertations, Fall 2023 to Present

Bighorn sheep populations across the western United States face increasing challenges from habitat loss, disease, and predation. In Zion National Park and surrounding areas, survey-based estimates indicate that the desert bighorn sheep population declined from over 800 individuals in 2018 to fewer than 500 in 2021. Understanding what drives these population changes is critical for effective wildlife management and conservation. This research examined key factors influencing population growth and individual survival to inform strategies for recovery in this bighorn population.

First, we analyzed which vital rates contributed most to population trends by estimating survival and reproduction rates from ground and …


The Impact Of Visually Presented Distractor Taboo Words During A Bilateral Attention Task, Chloe Taylor May 2025

The Impact Of Visually Presented Distractor Taboo Words During A Bilateral Attention Task, Chloe Taylor

All Graduate Theses and Dissertations, Fall 2023 to Present

Attention is a limited resource. Some events are able to capture one’s attention more efficiently than others, such as swear or sexual words. These events have preferential access to attentional resources and increase our arousal levels due to the activity of a neurotransmitter called norepinephrine. We investigate how well swear/sexual words capture peoples’ attention and interpret these effects in terms of norepinephrine activity.

Participants performed a visual task where they responded to different shapes presented while ignoring neutral or swear/sexual words presented on opposing sides of the computer screen.

Participants were significantly more inaccurate in identifying the shape present when …


Color Expressions In Euripides’ Ion, Erik Shelley May 2025

Color Expressions In Euripides’ Ion, Erik Shelley

All Graduate Theses and Dissertations, Fall 2023 to Present

This analysis explores color expressions in the play Ion by the ancient Greek playwright Euripides. Because the ancient Greeks experienced color differently, I examined words used in the play, including light, dark, fire, green, water, blood, and gold. These expressions imply different themes and concepts that Euripides uses to portray the duality between his two main characters, Ion and Creusa. This thesis showed how the mother-son pair acts as a foil for the different aspects of color found throughout the play that reflect ancient Greek culture.


Investigating Changes In Hydroclimate, Land Cover, And Evapotranspiration Across The Great Salt Lake Basin And Its Major Subbasins, Bhuwan Ghimire May 2025

Investigating Changes In Hydroclimate, Land Cover, And Evapotranspiration Across The Great Salt Lake Basin And Its Major Subbasins, Bhuwan Ghimire

All Graduate Theses and Dissertations, Fall 2023 to Present

The Great Salt Lake (GSL) is a lake of environmental and economic importance for Utah, and in recent years lake levels have declined to critically low levels. The lake level is dependent on the balance between the inflow and outflow. Inflow into the lake is governed primarily by the streamflow originating from the Bear, Weber and Jordan River basins (GSL subbasins), direct precipitation over the lake and groundwater entering into the lake. The GSL is a terminal lake, with outflow via evaporation from the lake.

In this study, we examined hydrologic processes within the river basins that relate to streamflow …


A Pavlovian, Conditioned-Reinforcement Approach To Reducing Impulsive Choice, Katherine Garland May 2025

A Pavlovian, Conditioned-Reinforcement Approach To Reducing Impulsive Choice, Katherine Garland

All Graduate Theses and Dissertations, Fall 2023 to Present

Persistent impulsive choice (the preference for a smaller-sooner over a larger-later reward) is associated with consequential life outcomes. Procedures that reduce nonhuman impulsive choice have long training durations that may reduce their translational utility. The purpose of this experiment was to test a new behavioral intervention to reduce impulsivity that requires few sessions to implement. Delay-bridging stimuli (stimuli presented during the delay to a reward) have been shown to reduce impulsive choice, but delay-bridging stimuli may also be aversive. Conditioned stimuli (CS) established through Pavlovian training are, on the other hand, attractive, potentially even functioning as a conditioned reinforcer (CR). …


The Desert Shall Rejoice: Lds Relational Values And Multispecies Justice In The Intermountain West, Sarah A. Woodbury May 2025

The Desert Shall Rejoice: Lds Relational Values And Multispecies Justice In The Intermountain West, Sarah A. Woodbury

All Graduate Theses and Dissertations, Fall 2023 to Present

In the American West, climate change is shifting weather and climate patterns in ways that may dramatically change lands, livelihoods, and communities. In the face of these threats, conservationists and local communities must work collaboratively to ensure that environmental and human communities are healthy enough to weather approaching shifts. Church of Jesus Christ of Latter-day Saint (LDS) values, practices, and histories strongly influence the Intermountain West, yet there is little research on LDS environmental values and perceptions. This study seeks to answer questions that work in this gap. It documents the knowledge and values of communities in the Intermountain West …


Screening Of A Diversity Of Legumes And Non-Legumes Forb Species At Five Strategic Locations Across Utah For The Development Of Smart Foodscapes, Surbhi Verma May 2025

Screening Of A Diversity Of Legumes And Non-Legumes Forb Species At Five Strategic Locations Across Utah For The Development Of Smart Foodscapes, Surbhi Verma

All Graduate Theses and Dissertations, Fall 2023 to Present

Grass-dominated rangelands in the Western United States often lack sufficient protein for pregnant cows after grasses mature in mid- summer, leading ranchers to rely on expensive feed supplements such as protein cubes or alfalfa hay. The goal of this study was to screen 27 perennial legume and forb species with the potential to remain green into late summer and provide sufficient protein to supplement cows on grass-dominated rangeland. Three experiments were carried out to understand germination of species, plant establishment and persistence at five Utah locations, and agronomic characteristics. The seed of five hard-to-germinate species were studied for the ability …


Engineering Planktonic And Biofilm Microalgae Systems For Productivity, Power Requirements, Cost, And Scalability, Dallin Chugg May 2025

Engineering Planktonic And Biofilm Microalgae Systems For Productivity, Power Requirements, Cost, And Scalability, Dallin Chugg

All Graduate Reports and Creative Projects, Fall 2023 to Present

Microalgae cultivation offers dual benefits as an effective wastewater treatment method and a sustainable source of valuable bioproducts, such as biofuels, bioplastics, and feedstocks. This approach supports sustainability by transforming waste into high-value products while minimizing environmental impact. However, scaling microalgae cultivation to industrial levels presents significant challenges, particularly in optimizing harvesting efficiency, reducing mixing and transport costs, and managing large water requirements.

This report compares the productivity, scalability, cost, and energy requirements of two primary cultivation systems: planktonic and biofilm. Planktonic systems, such as open raceway ponds (ORPs), excel in light distribution and nutrient uptake due to continuous mixing, …


Evaluating The Economic Viability Of Feeding Hydroponic Barley Fodder To Growing Wethers, Fallon Johnson May 2025

Evaluating The Economic Viability Of Feeding Hydroponic Barley Fodder To Growing Wethers, Fallon Johnson

All Graduate Reports and Creative Projects, Fall 2023 to Present

As the livestock industry combats challenges including rising feed costs and ensuring sustainable production practices, some attention is being given to hydroponic fodder as a feed source. Hydroponic fodder offers a potential alternative to traditional feed sources. Research has demonstrated hydroponic fodder production has increased water efficiency while providing a sustainable green feed source throughout the year. This study seeks to understand how using hydroponically produced barley fodder in a total mixed ration for growing wethers compares economically to feeding traditional feed sources. A stochastic simulation model is used to determine the expected total feed costs, costs of gain, and …


Gravity Wave Breaking Over Northern Utah With An Advanced Mesospheric Temperature Mapper And Sodium Lidar, Eric Davis May 2025

Gravity Wave Breaking Over Northern Utah With An Advanced Mesospheric Temperature Mapper And Sodium Lidar, Eric Davis

All Graduate Reports and Creative Projects, Fall 2023 to Present

Considerable progress has been made in recent decades in the understanding of gravity wave (GW) dynamics. However, there remain important aspects and effects that are poorly understood. In particular, the coupling and deposition of their energy and momentum into the mesosphere lower thermosphere (MLT) region and the interaction and instability dynamics that are directly associated with GW breaking. Using an Advanced Mesosphere Temperature Mapper (AMTM) several strong wave breaking events were observed in the summer of 2015 at USU’s BLO research station. These events have exhibited high momentum fluxes and associated strong wave breaking, prompting detailed data analyses. Rossby waves, …


"Death Is The Fifth [Season], And Master Of All": Necropolitical Violence And/As Environmental Violence In The Fifth Season, Mason T. Goodrich May 2025

"Death Is The Fifth [Season], And Master Of All": Necropolitical Violence And/As Environmental Violence In The Fifth Season, Mason T. Goodrich

All Graduate Reports and Creative Projects, Fall 2023 to Present

While many scholars have already described N.K. Jemisin’s Hugo Award-winning speculative novel The Fifth Season as one that highlights intersections between race, settler-colonialism, neoliberalism, and, most frequently, climate change, I apply a necropolitical lens to The Fifth Season in order evaluate a relationship that exists between systemic violence and environmental degradation. The Fifth Season depicts a socially inscribed relationship between an othered race of humans, called orogenes, and the land, anthropomorphized as “Father” Earth. Orogenes have a paradoxical social status: they are simultaneously feared and revered for their ability to control the Earth. This paradox is the impetus for many …


“Floptok Is Not A Trend. It’S A Lifestyle”: Camp, Performativity, And Community Dynamics In An Adolescent Tiktok Subculture, Drake Hansen May 2025

“Floptok Is Not A Trend. It’S A Lifestyle”: Camp, Performativity, And Community Dynamics In An Adolescent Tiktok Subculture, Drake Hansen

All Graduate Reports and Creative Projects, Fall 2023 to Present

Floptok is an online meme-sharing community or subculture that began to take shape in 2021, united by a particular sensibility and set of memetic materials. To outsiders, Floptok is seen as a loud, brash, and often abrasive community whose memes, characterized by low-quality audio and video, often feature “icons”—a range of invented characters and real people—and audio of pop songs heavily edited to feature hypersexual lyrics from the rapper CupcakKe, as well as portions of the 2019 Chinese pop song “Yěhuāxiāng.” This thesis built on scholarship in digital and young people’s folklore in an ethnographic case study of Floptok’s humor …


Motherhood Within The Patriarchal Family: An Analysis Of “Frankenstein” And “The Scarlet Letter”, Yasamin Osqueezadeh May 2025

Motherhood Within The Patriarchal Family: An Analysis Of “Frankenstein” And “The Scarlet Letter”, Yasamin Osqueezadeh

Undergraduate Honors Capstone Projects

Being a pivotal period in Western feminist history, the literary community in the 18th century introduced pioneering feminists, the most famous of which is Mary Wollstonecraft, who called for a feminist revolution with A Vindication of the Rights of Women. Following the publication of A Vindication of the Rights of Women and other subsequent feminist papers, are Mary Shelley’s 1818 Frankenstein and Nathaniel Hawthorne’s 1850 The Scarlet Letter, two renowned pieces of Gothic literature. Although the two novels may seem disconnected, both Frankenstein and The Scarlet Letter engage in feminist arguments and critiques of the patriarchy in …


Investigation Of Immune/Performance Trade-Offs In Neonate Children's Pythons (Antaresia Childreni), Ellie Hartman May 2025

Investigation Of Immune/Performance Trade-Offs In Neonate Children's Pythons (Antaresia Childreni), Ellie Hartman

Undergraduate Honors Capstone Projects

Energetic costs and constraints are key considerations when analyzing an organism’s life history and may require trade-offs to be made between different life history traits. One of these trade-offs occurs between investment in immunity and performance. The purpose of this study is to discover if this trade-off influences behavior in neonate Children’s pythons (Antaresia childreni). We hypothesize that snakes undergoing an immune response will have reduced behaviors promoting survival. To test this hypothesis, neonate Children’s pythons were randomly assigned to receive an immune challenge of keyhole limpet hemocyanin (KLH) or a vehicle injection as a control. Two days …


Adaptation Of Influenza A Virus Strains A/Anhui/1/2013 (H7n9) And A/Shanghai/1/2013 (H7n9) For Development Of Effective Mouse Models, Spencer T. Mullen May 2025

Adaptation Of Influenza A Virus Strains A/Anhui/1/2013 (H7n9) And A/Shanghai/1/2013 (H7n9) For Development Of Effective Mouse Models, Spencer T. Mullen

Undergraduate Honors Capstone Projects

The emergence of avian Influenza A (H7N9) virus strains in humans poses a significant threat to human health globally due to their pandemic potential, limited human immunity, and lack of virus-specific interventions. This capstone aims to address some of the limitations in antiviral research regarding Influenza A H7N9 virus by adapting two low-pathogenic virus strains A/Anhui/1/2013 (H7N9) and A/Shanghai/1/2013 (H7N9) to BALB/c mice through sequential passaging through lung tissue. Following 12 serial passages, increased virulence was observed as evidenced by increased virus replication, increased weight loss, and increased mortality in infected mice. Titration of the initial non-adapted virus isolates were …