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Exocytosis From Large Dense Cored Vesicles As A Mechanism For Neuropeptide Release In The Peripheral And Central Nervous System, Asa Thureson-Klein, Richard L. Klein, Pei-Chun Zhu

Scanning Electron Microscopy

Nerve terminals often contain morphological-distinct populations of large (75-110 nm) and small (45-55 nm) vesicles. The small vesicles are speculated to account for release of transmitter quanta as they accumulate at presynaptic membranes. Large vesicles can co-store neuropeptides and classical transmitters but their function in neurotransmission has been disputed because they do not appear to accumulate at chemical synapses. However, there is now evidence that the large vesicles play a role in neuro-transmission or its modulation even though they may not be eminently involved in synaptic release. Thus, exocytosis occurs along the synapse-lacking membranes of peripheral noradrenergic varicosities. Large vesicles …

Insites, Laep Week 1986, Utah State University

inSites

Magazine of the Department of Landscape Architecture and Environmental Planning

LAEP week special edition published by the student chapter of ASLA at USU

Utah Science Vol. 47 No. 1, Spring 1986

Utah Science

2 ANNUAL FLOWERS W. A. Varga Looking for ways to add color and interest to the landscape? A USU horticulturist reviews the attributes of some annual flowers.

6 UNDERSTANDING PLANT-MICROBE COMMUNICATION Anne Anderson Eavesdropping on the "conversations" between plants and microbes may help researchers genetically engineer plants more resistant to pathogens and that can better utilize beneficial microorganisms.

9 WHOLE MILK OR MILK REPLACERS: A NUTRITIONAL AND ECONOMIC COMPARISON S. L. Maxfield. J. L. Walters, R. D. White, and S. D. Allen Even though prices for whole milk have declined in recent months, it still isn't economical to substitute marketable …

Calcium And Cystic Fibrosis, Godfried M. Roomans

Scanning Electron Microscopy

Cystic fibrosis (CF) is a generally lethal, congenital, genetic disease of unknown etiology. It is likely that a defective regulation of ion and water transport in exocrine glands and possibly also in other epithelial cells has a central role in the pathogenesis of this disease. Calcium has been implicated in the basic defect underlying CF because of findings of abnormally high calcium levels in some secreted fluids and some cells of CF patients.

Using X-ray microanalysis, we have demonstrated elevated calcium concentrations in cultured fibroblasts and in goblet cells of the bronchial epithelium of CF patients. A factor produced by …

Quantitative Renal Vascular Casting In Nephrology Research, Vincent H. Gattone Ii, Andrew P. Evan

Scanning Electron Microscopy

The present paper describes the use of a quantitative renal vascular casting method to study the changes associated with kidney disease. Several animal models of hypertension (spontaneously hypertensive rat, SHR, with its normotensive rat the Wistar Kyoto, WKY; Dahl salt sensitive DS - hypertensive, and salt resistant DR -normotensive) were examined at time points when the systemic blood pressure was rising (6 and 12 weeks of age) and following renal denervation (in SHR-WKY rats). The SHR appears to have a smaller caliber afferent arteriole at both 6 and 12 weeks of age. This difference is probably not entirely due to …

Plant Ultrastructure In The Scanning Electron Microscope, Susan H. Barnes, Stephen Blackmore

Scanning Electron Microscopy

Preparative techniques which have been used to study internal details of plant cells in the scanning electron microscope are reviewed. A number of methods have previously been described which involve selective extraction of materials from freeze-fractured surfaces and can be referred to as freeze-fracture and cytoplasmic maceration. One of these techniques which involves an extended period of cytoplasmic maceration with dilute osmium tetroxide has been applied to the study of Cichorium intybus (chicory) pollen ontogeny. The results obtained, including changes in the numbers of mitochondria and the form of endoplasmic reticulum during the course of development demonstrate the value of …

Cytoskeletal Changes During Adhesion And Release: A Comparison Of Human And Nonhuman Primate Platelets, J. C. Lewis, M. S. White, T. Prater, K. R. Porter, R. J. Steele

Scanning Electron Microscopy

The organization of cytoskeletal proteins in whole-mount adherent platelets from African green monkeys and normal human volunteers has been studied by SEM, high vacuum electron microscopy (HVEM) and conventional (120 kV) electron microscopy. We describe three distinct organizational zones, the Central Matrix, the Trabecular Zone and the Peripheral Web in spread platelets from both sources. The Central Matrix is an ill-defined superstructure of 80-100 Å filaments of short length which enshrouded the granules, dense bodies, mitochondria and elements of the open-channel and dense-tubular systems. The latter, identified through the use of peroxidase cytochemistry with the whole mounts, is an anastomosing …

Determination Of Internal Color Of Beef Ribeye Steaks Using Digital Image Analysis, K. Unklesbay, N. Unklesbay, J. Keller

Food Structure

Objective measurements of beef ribeye steaks were made to determine the color distribution throughout their interior after heat processing. Steaks from eight animals were grilled to five degrees of doneness according to traditional internal temperature specifications. Images of the interior of these steaks, as seen by a digital image processing system through red, green, and blue filters were analyzed. The mean, standard deviation, skewness, and kurtosis of the histograms of light intensity at all points throughout the surface were determined. The steaks were also analyzed raw and it was determined that little variation in the color of muscle tissue occurred …

Wild Mustangs, Parley J. Paskett

All USU Press Publications

A mustang is a wild horse, a broomtail, a cayuse, a fantail, or any of several other terms cowboys use to describe this tame animal gone wild. To you, my children, and your children and theirs, I give this book of stories about mustangs. The action and excitement I experienced while capturing mustangs are described here.

Using helicopters today to run down wild horses removes both the thrill and sport in their capture. It was surely more exciting and certainly a greater challenge to pit a saddle horse and rider against the fear and speed of a wild horse. The …

Variation In Individual Growth Rates And Population Abundances Of Ephemerellid Mayflies, Charles P. Hawkins

Watershed Sciences Faculty Publications

Individual growth and densities of six taxa of ephemerellid mayflies were examined in relation to differences in temperature and food among streams of western North America. For most taxa, growth rate was not a simple function of either temperature or food. Growth periods of taxa differed relative to seasonal changes in temperature. As a result of the interaction between size and temperature, growth rates and shapes of the growth curve varied among taxa. The significance of temperature for life history phenomena therefore cannot be easily generalized to explain phenological patterns among stream insects. Also, little evidence was found that implicated …

Proceedings From The 23rd Annual Marschall Invitational, Various Authors

Cheese Industry Conference

No abstract provided.

Mountain Pine Beetle Outbreaks In The Rocky Mountains: Regulators Of Primary Productivity?, W. H. Romme, D. H. Knight, J. B. Yavitt

The Bark Beetles, Fuels, and Fire Bibliography

We consider the hypothesis that mountain pine beetles function as cybernetic regulators of primary productivity in ecosystems of lodgepole pine forest through their selective killing of dominant trees and the subsequent redistribution of resources. Following a recent major beetle outbreak in Yellowstone and Grand Teton national parks, surviving trees did grow significantly faster (P < .1); wood production was redistributed among canopy, subcanopy, and understory trees; and annual wood production per hectare usually returned to pre-attack levels or exceeded them within 10-15 yr. However, reconstructions of annual wood production over the last 70-80 yr indicate that the beetle outbreak did not reduce the variation in productivity; rather, the beetles introduced more variation than would have existed in their absence. Hence, our results do not support the hypothesis that the beetles function as cybernetic regulators (in the strict sense). Nevertheless, the beetle-pine system that we studied shows great resilience, and the effects of beetles on primary productivity do not appear to be as severe as conventional wisdom maintains. Annual wood production per hectare returned quickly to previous levels in the stands we studied, and associated ecological changes can be considered generally benign or even beneficial.

The Microstructure Of The Hen's Egg Shell - A Short Review, R. M. G. Hamilton

Food Structure

The structure of the hen's egg shell can be divided into five separate layers . The Innermost layer consists of two distinct membranes known as the inner and outer shell membranes. These membranes are compos ed of networks of protein/polysaccharide fibres and are =70 mm thick. Attached to the outer fibres of the outer membrane are polycrystals of calcite (calcium carbonate) which extend outward in an inverse conical manner until the cones from several sites of crystal i nit iation fuse together. The fibre/ crystal attachment sites, known as basal caps, and the cones form the mammillary knobs layer which …

Accuracy And Utility Of Sarcomere Length Assessment By Laser Diffraction, Peter A. Koolmees, Frank Korteknie, Frans J.M. Smulders

Food Structure

In two experiments the computation of sarcomere length from laser diffraction patterns was tested for accuracy against phase-contrast microscopy . Particular attention was paid to methodological factors such as sampling location and computation formulae. Correlation coefficients between the laser diffraction technique and the microscopical method were high (r= 0.96) in both experiments. However, when computed from a simplified formula, the sarcomere length values, determined by laser diffraction patterns tended to be approximately 0. 10 lJm lower. It is recommended to use the correct formula in computing the laser diffraction data and to investigate a limited number of fibres (3) in …

Food Microstructure

Food Structure

No abstract provided.

Freeze Texturation Of Proteins: Effect Of The Alkali, Acid And Freezing Treatments On Texture Formation, Frisco I. Consolacion, Paul Jelen

Food Structure

The effects of alkali extraction, isoelectric precipitation, and rate of ice crystal formation on protein interaction in the freeze texturization process were studied , The protein isolate was obtained from mechanically deboned poultry meat residues by extraction with NaOH at pH 10.5 and precipitation by HCl at pH 5, 0, The presence of large molecular weight subunits in the protein isolate was revealed by SDS-polyacrylamide gel electrophoresis. Scanning electron microscopy demonstrated the nature of the fiber formation and protein cross-linking as affected by freezing at -25'C or -196'C. The transformation of the level of protein organization from the lumpy or …

Progress Report For The Dairy Research Advisory Board, Various Authors

Other Documents

No abstract provided.

Current Concepts Of Muscle Ultrastructure With Emphasis On Z-Line Architecture, M. Yamaguchi, H. Kamisoyama, S. Nada, S. Yamano, M. Izumimoto, Y. Hirai, R. G. Cassens, H. Nasu, M. Muguruma, T. Fukazawa

Food Structure

Invertebrate striated muscle, the Z-line, which defines the sarcomere length, presents diverse structural patterns both in cross section and in longitudinal section. Conflicting models have been proposed to explain the microscopic observations. The protein composition of the Z- line structure is unresolved. o: -Actin in is widely accepted as a Z-line component, and actin filaments extend into wide Z-lines. Based on recent findings from our laboratory and others, we developed a new model applicable to wide and narrow Z-lines. The model allowed the observed ultrastructural patterns of Z-lines to be simulated. Improved electron microscopic techniques should allow further progress to …

Food Microstructure

Food Structure

No abstract provided.

Seventh Biennial Cheese Industry Conference, Various Authors

Cheese Industry Conference

No abstract provided.

The Influence Of Viewing Angle On Elk Hiding Cover In Young Timber Stands, United States Department Of Agriculture, Forest Service

Forestry

Young timber stands, even when providing 100 percent visual concealment for elk when viewed on a horizontal plane, may provide 50 percent or less cover when viewed from an opposing slope at an elevated viewing angle. The higher the viewing angle, the greater the relative cover loss. In a simple linear model, viewing angle explained 52 percent of the variation in hiding cover values. Slightly more variation was accounted for when the data were stratified by tree height. On the average, for a 10-degree elevation in viewing angle, hiding cover decreased by 10 percent. The cover loss relationship was most …

'Hobble Creek' - A Superior Selection Of Low-Elevation Mountain Big Sagebrush, United States Department Of Agriculture, Forest Service

Forestry

This is the foundation document for the release of the 'Hobble Creek' selection of big sagebrush. It is a low-elevation mountain big sagebrush (Artemisia tridentata ssp. vaseyana). 'Hobble Creek' is needed to increase the nutrient content of winter diets of mule deer (Odocoileus hemionus hemionus) and domestic sheep (Ovis aries). This sagebrush exceeds the typical winter forage values in amount of energy-producing compounds, crude protein, phosphorus, and carotene. Of the 186 big sagebrush selections tested. 'Hobble Creek' is the most preferred by wintering mule deer and ranks high in preference by wintering domestic sheep. 'Hobble Creek' does not contain substances …

Proceedings -- National Wilderness Research Conference: Current Research, United States Department Of Agriculture, Forest Service

Forestry

Includes more than 70 reports of current wilderness research. Papers are organized around nine topics: wilderness resource research, including natural fire, air quality, impacts to soil and vegetation, fish and wildlife, and water; and wilderness user research related to recreational use and user characteristics, attitudes and behavior, benefits, and management concepts and tools.

Visitor Attitudes Toward Wilderness Fire Management Policy -- 1971-84, United States Department Of Agriculture, Forest Service

Wildfires

The historical role of naturally occurring fire in shaping the character of many American landscapes has become an accepted ecological principle. Prior to the coming of the Europeans, natural fires had a major influence in producing a variety of vegetational mosaics. Fire suppression policies of the 20th century, however, significantly changed the evolution of many landscapes compared to what would have occurred had natural fires been allowed to continue. Although natural fires can no longer be allowed to burn in many places because of resource values or danger to the public, such fires may be permitted in many wildernesses and …

Bats, Terry A. Messmer, E. Cleary

Wildland Resources Faculty Publications

No abstract provided.

The Skinned Fiber Technique As A Potential Method For Study Of Muscle As A Food, R. G. Cassens, T. J. Eddinger, R. L. Moss

Food Structure

Skeletal, smooth and cardiac muscle cells can be skinned by physical means or a variety of chemical techniques. The skinned fibers have been used to study the molecular mechanisms of contraction and the regulation of contraction by ca++. Skinned fiber preparations are also useful for study of muscle as a food. For example, it is now possible to determine fiber type of skinned fibers following study of their physical properties.

Fixing Conditions In The Freeze Substitution Technique For Light Microscopy Observation Of Frozen Beef Tissue, M. N. Martino, N. E. Zaritzky

Food Structure

The freeze substitution histological technique allows the indirect light microscopicobservation and the quantitative evaluation of ice crystal size in frozen tissues. Theuseofchemical fixatives in substitu ting fluids improves morphological and histochemical preservation of the tissue. Fixation conditions become important since this step can introduce modifications in crystal sizes . Effects of temperature on: a) diffusion rate of fixing solution in tissue, b) recrystallization rate of ice in frozen beef and c) variations of the froze~ water fraction were ana lyzed, establishing that 1sothermal freeze fixation constitutes an appropriate method for histological observation. Fixing at temperatures lower than that of the …

Microstructure Of Mealy And Vitreous Wheat Endosperms (Triticum Durum L.) With Special Emphasis On Location And Polymorphic Behaviour Of Lipids, A. Al Saleh, D. Marion, D. J. Gallant

Food Structure

Dark inclusions observed in osmiophilic zones, already described in mature wheat endosperm using transmission electron microscopy, were confirmed by freeze-fracture electron microscopy to be lipids. The polar lipids (glycolipids, phospholipids, free fatty acides, monoglycerides) were organized in a liquid crystalline phase. The reversed hexagonal or HII phase should be the main lattice which might arise from the transition of lipids present in membranous structures as a lamellar phase. This transition was caused by dehydration occurring during maturation. It is suggested that the water-dependent lamellar hexagonal phase transitions are of considerable importance in cereal food technology.

Features Of Food Microscopy, D. F. Lewis

Food Structure

The value of food microscopy is judged by its practical application to food handling. Hence microscopists must explain the relevance of their findings to food technologists. The food microscopist has to deal with materials that are particularly difficult to prepare for microscopy because they often contain high levels of fat, air, sugar, salt, starch or acid. Sometimes the methods used are unorthodox and could be regarded as questionable by microscopists in more traditional disciplines. This paper considers the relationship between food microscopists, food technologists and other microscopists. An approach to interpretation of images is based on the following features. 1) …

Utah Science Vol. 47 No. 4, Winter 1986

Utah Science

110 REAL-TIME ULTRASOUND: A NEW WAY TO STUDY THE EFFECTS OF POISONOUS PLANTS T. D. Bunch, K. E. Panter and L. F. James Ultrasound is used to monitor normal fetal growth and learn how poisonous plants affect fetal development

114 IMPROVING SYMBIOTIC NITROGEN FIXATION K. A. Lawson, D. A. Johnson and M. D. Rumbaugh Plant and bacterial combinations from Pakistan and Spain may improve the nitrogen-fixing capacity of alfalfa on western rangelands.

120 INSULIN-DEPENDENT DIABETES MELLITUS: A STUDY OF NUTRIENT INTAKE B. M. Prater The food intake of persons with insulin-dependent diabetes appears to meet the RDAs for nutrients.

124 …