Digital Commons Network^™

Dolores Archaeological Program: Anasazi Communities At Dolores: Middle Canyon Area, Book 2 Of 2, United States Department Of The Interior, Bureau Of Reclamation

Archaeology/Anthropology

This volume is a collection of published reports from the Middle Canyon area of the Dolores Project in southwestern Colorado. Included in the volume are 4 site reports and 3 analytical chapters produced by the Dolores Archaeological Program. This volume includes information on the environmental setting of the Middle Canyon area, a summary of Dolores Archaeological Program systematics, and a brief description of Dolores cultural history.

Dolores Archaeological Program: Anasazi Communities At Dolores: Middle Canyon Area, Book 1 Of 2, United States Department Of The Interior, Bureau Of Reclamation

Archaeology/Anthropology

This volume is a collection of published reports from the Middle Canyon area of the Dolores Project in southwestern Colorado. Included in the volume are 4 site reports and 3 analytical chapters produced by the Dolores Archaeological Program. This volume includes information on the environmental setting of the Middle Canyon area, a summary of Dolores Archaeological Program systematics, and a brief description of Dolores cultural history.

The Biomineralization In Social Wasps (Vespinae): The Presence Of Statoliths, J. S. Ishay, T. Shimony, L. Arcan

Scanning Electron Microscopy

In social wasps granular aggregates occur mainly in the head region and the body integument. In the former location they are present both on the outer and inner side of the frons. On the outer side of the frons, in a groove traversing its center, there is a deep pit surrounded by a membrane which forms a cyst-like sac. In the pit of the hornet Vespa orientalis (Linne, 1771), there are aggregates comprising silicon (Sl), potassium (K), calcium (Ca) and iron (Fe), which are arranged in morula-like fashion. A granular material is secreted at the base of sensory hairs …

Permanent Noise-Induced Damage To Stereocilia: A Scanning Electron Microscopic Study Of The Lizard's Cochlea, Michael J. Mulroy

Scanning Electron Microscopy

Alligator lizards were exposed to broadband noise ranging in intensity from 106 to 132 dB SPL for two hours and permitted to recover from 19 to 62 days. Hearing loss was assessed by comparing the auditory nerve component of the cochlear potential recorded at the end of the recovery period with that recorded before the noise exposure. The stereocilia in these ears were examined with a scanning electron microscope. These sensory hairs showed pathological changes similar to those described in mammalian cochleas with noise-induced damage. In decreasing order of severity the damage included completely missing auditory papillas, missing hair cells, …

Genes, Manganese, And Zinc In Formation Of Otoconia: Labeling, Recovery, And Maternal Effects, L. C. Erway, N. A. Purichia, E. R. Netzler, M. A. D'Amore, D. Esses, M. Levine

Scanning Electron Microscopy

Published studies indicate that genes and dietary manganese deficiency cause vestibular defects and ataxic behaviors. Manganese deficiency during development causes otoconial defects in mice, rats, guinea pigs, and chicks. Mutant genes cause otoconial defects in mice, mink, and poultry. Manganese supplementation prevents the otoconial defects in some mutant mice and mink. Manganese is essential, before crystallization of the otoconia, for synthesis of mucopolysaccharides and otoconial matrix. Such defects can be induced, after otoconia are crystallized during fetal development, by dietary zinc deficiency and sulfonamide treatment (inhibits carbonic anhydrase, a zinc-requiring enzyme). Manganese and/or zinc supplementation ameliorates otoconial defects in pallid …

Topical Modes In The Preparation Of Human Spleen Specimens For Routine Scanning Electron Microscopy Studies, Haim Gamliel, Harvey M. Golomb, Eliezer Avinoah, Dorit Gurfel, Aaron Polliack

Scanning Electron Microscopy

Various preparatory techniques were used to improve scanning electron microscopy images of the fine structure of vascular, cellular, and cordal-reticular components of normal human spleens. The progressive method of fixation (GTGO) applied in the present study, allowed air drying of the tissues and rendered the specimens conductive even in newly fractured surfaces. Vascular perfusion proved necessary only in studies of the splenic blood vessels, while a simple immersion of tissue blocks in the washing solution resulted in better images of the white pulps. Interstitial (trans-splenic) perfusion was found to be superior to vascular perfusion for routine preparation of spleen tissues, …

Particle Probe Analysis In The Study Of Skin Physiology, Bo Forslind

Scanning Electron Microscopy

The basic function of provide a barrier which the epidermis is will separate the body compartment from the environment thus protecting the organism from excessive loss of water and to hinder the entrance of noxious agents. A continuous renewal of the actual barrier makes it possible to fulfill these requirements. Using particle probe analysis, electron microprobe (EMP) and proton microprobe (PMP) analysis we have demonstrated the feasibility of these techniques in the study of skin physiology. The results reported here have been obtained on quench frozen skin specimens inertly prepared by cryotechniques to produce freeze-dried sections presenting cross sections of …

Scanning Electron Microscopic Study Of The Surface Of Feline Gastric Epithelium: A Simple Method Of Removing The Coating Material, M. Al-Tikriti, R. W. Henry, F. K. Al-Bagdadi, J. Hoskins, C. Titkemeyer

Scanning Electron Microscopy

Scanning electron microscopic examination of the gastric surface epithelial cells is often hindered by the presence of a coating material. Several methods for removal of coating material on feline gastric mucosa were utilized. The cleansed tissues were evaluated using the scanning electron microscope to assess damage caused by the use of various cleansing methods to surface epithelial cells. The stretched stomach washed several times, including rubbing the mucosal surface with gloved fingers, yielded the best results with no apparent damage to the surface epithelial cells. Flushing unstretched stomachs with saline only did not adequately remove coating material. Flushing unstretched stomachs …

Calcification In Aging Canine Aortic Valve, Kookmin M. Kim, S. H. Chang, B. F. Trump, H. Spurgeon

Scanning Electron Microscopy

Aging changes of aortic valves are thought to underlie the mechanism of calcification, which leads to calcific aortic stenosis in humans. The study of calcification in the aging valvular connective tissue has been hindered by the lack of a suitable animal model. In search of the model, canine aortic valves demonstrated age changes including calcification remarkably similar to those in humans. The mechanism of calcification was studied in the aortic valves of aged Beagles by electron microscopy. Fibroblasts in the canine aortic valves showed the most prominent age changes. The cells accumulated numerous residual bodies and appeared to disintegrate. The …

Scanning Electron Microscopic Features Of Spleen In The Rat And Human: A Comparative Study, S. Sasou, R. Satodate, T. Masuda, K. Takayama

Scanning Electron Microscopy

The marginal zone, white pulp and red pulp of rat and human spleen were studied by scanning electron microscopy and were compared. The marginal zone was observed in both species. The arterial termination in the marginal zone was quite different between both species. The follicular arteries terminated at the boundary of the white pulp and formed a vascular net regarded as the marginal sinus in rat. On the other hand, numerous arterial termini of the follicular and sheathed arteries were scatteringly found in the marginal zone in man. The central artery was surrounded with flat reticular cells in rat and …

Characterization Of Mucus Glycoconjugates In Normal Human Conjunctiva By Lectins In Light Microscopy, Transmission And Scanning Electron Microscopy, P. Versura, M. C. Maltarello, F. Bonvicini, R. Caramazza, R. Laschi

Scanning Electron Microscopy

Maintenance of tear film in normal conditions is dependent on 1) mucus layer integrity and 2) the presence and distribution of conjunctival epithelial cell microvilli.

In the present work a new methodology has been developed to gain correlative information about microprojection assessment and mucus composition, from the same specimen, by Light Microscopy (LM), Transmission Electron Microscopy (TEM) and Scanning Electron Microscopy (SEM).

We have characterized the glycosidic residues secreted by goblet cells in normal human conjunctiva, by means of four lectins (WGA, ConA, PNA and SBA), conjugated with FITC for LM and with colloidal gold for TEM and SEM.

The …

Adhesion, Spreading And Fragmentation Of Human Megakaryocytes Exposed To Subendothelial Extracellular Matrix: A Scanning Electron Microscopy Study, Y. G. Caine, I. Vlodavsky, M. Hersh, A. Polliack, D. Gurfel, R. Or, R. F. Levine, A. Eldor

Scanning Electron Microscopy

Platelet agonists and subendothelial extra-cellular matrix (ECM) induce morphological and biochemical changes in animal megakaryocytes, reminiscent of the response of platelets to the same substances. We have examined the behavior of human megakaryocytes exposed for up to 36 hours to the ECM produced by cultured bovine corneal endothelial cells. By phase contrast and scanning electron microscopy these megakaryocytes demonstrated non-reversible adherence and flattening with formation of long filopodia, thus confirming that human megakaryocytes acquire platelet functional capacities. In addition, megakaryocyte fragmentation into prospective platelets was apparently induced by the ECM. Up to 50% of the adherent megakaryocytes underwent spontaneous fragmentation …

Struvite Crystalluria And Urolithiasis In Cross Labradors, A. L. Rodgers, R. A. Irving, M. Mezzabotta, P. Benloulou

Scanning Electron Microscopy

Recurrent struvite crystalluria and urolithiasis in a Cross-Labrador bitch was studied using a combined Coulter-Counter and scanning electron microscope (SEM) approach. Staphylococcus bacteria were cultured from the patient's urine as well as from the calculi themselves. Urine samples were subjected to particle counting and sizing during active and non-active periods of stone formation. Size distribution curves so obtained were identical as were those derived from sterile and non-sterile specimens. These showed a peak incidence at a diameter of 5 μm. Particle sizes for 6 controls were also determined and showed an even distribution over a much wider range with small …

Effects Of Endotoxin On The Splenic Microcirculation And Its Cellularity, L. T. Chen

Scanning Electron Microscopy

This report describes the effects of endotoxin treatment on the intrasplenic microcirculation and cellularity in rats. Four and 16 h after a single intravenous injection of endotoxin (2 mg/100g body weight), altered intrasplenic microcirculation was observed. The open circulation was reduced from 97% in the control rats to 79% in the endotoxin treated rats, while the closed circulation increased from 3% in the controls to 21% in the endotoxin treated rats. Such changes in the splenic microcirculation may be partly due to the presence of fibrin and the pooling of polymorphonuclear leukocytes and red blood cells in the red pulp. …

Progress Report For The Dairy Research Advisory Board, Various Authors

Other Documents

No abstract provided.

The Three-Dimensional Microstructure Of The Liver A Review By Scanning Electron Microscopy, G. Macchiarelli, P. M. Motta

Scanning Electron Microscopy

The improvement in scanning electron microscopy (SEM) techniques has permitted us to describe the microstructure of the liver. By SEM, the liver peritoneal surface is composed of flat mesothelial cells possessing microvilli and cilia. Hepatic sinusoids connect the portal vessels with the terminal branches of the hepatic vein (central veins). Endothelial cells of the portal space arteries are elongated and arranged longitudinally, while those of the central and portal veins are polygonal and flattened, possessing microvilli. The sinusoidal endothelial cells show both small fenestrations (sieve plates), up to 200 nm in diameter, and large ones, up to 1 𝝁m. Within …

Proton Microprobe Analysis In Biology, Klas G. Malmqvist

Scanning Electron Microscopy

This paper is intended as an introduction to the field of proton microprobe analysis with special emphasis on applications in biological sciences. It is mainly intended for users of electron microscopes equipped with microprobes or other analytical equipment. The basic principles of Particle Induced X-ray Emission analysis are discussed as well as the instrumental requirements for the proton microprobe. The analytical characteristics including quantification procedures are compared with those of the electron microprobe and a review is given of various analytical applications of the proton microprobe within biology and medicine.

The Morphologic Categorization Of Cell Death Induced By Mild Hyperthermia And Comparison With Death Induced By Ionizing Radiation And Cytotoxic Drugs, David J. Allan, Brian V. Harmon

Scanning Electron Microscopy

This paper presents 1. a summary of the morphological categorization of cell death, 2. results of two in vivo studies on the cell death induced by mild hyperthermia in rat small intestine and mouse mastocytoma, and 3. a comparison of the cell death induced by hyperthermia, radiation and cytotoxic drugs.

Two distinct forms of cell death, apoptosis and necrosis, can be recognized on morphologic grounds. Apoptosis appears to be a process of active cellular self-destruction to which a biologically meaningful role can usually be attributed, whereas necrosis is a passive degenerative phenomenon that results from irreversible cellular injury.

Light and …

Ultrastructure Of Bone Marrow Of Rats After Severe Hemodilution With Starch Or Modified Hemoglobin, Ferrell R. Campbell, Richard D. Rink

Scanning Electron Microscopy

The ultrastructure of bone marrow of rats was studied 24 h after exchange-transfusion with solutions of starch or modified hemoglobin to a hematocrit of 10-15. Blood smears of the transfused rats had 17-20% reticulocytes as compared to 5-6% for sham operated controls. In the transfused rats marrow macrophages had numerous heterolysosomes apparently containing the starch or hemoglobin from the transfused solutions. Endothelial cells and reticular cells also possessed a few heterolysosomes thought to contain starch or hemoglobin. Reticular cells of the transfused rats contained numerous glycogen particles scattered throughout the cytoplasm or arranged in large masses. Synthesis of glycogen may …

Microcorrosion Casting Of The Human Respiratory Acinus, S. A. Dilly

Scanning Electron Microscopy

Microcorrosion casts demonstrating the respiratory acini of human lungs were produced using Tensol Cement No. 70 (a methyl methacrylate mixture). Lung casts were made from foetuses of 19 weeks' gestation to term, a child of 5 years and two adults. Tensol Cement No. 70 was found to infuse the most peripheral airspaces without tissue penetration and could be airdried. Attempts using Batson's medium were hampered by permeation of the airway walls and inability to withstand airdrying. Despite the use of autopsy material, good cell impressions were seen.

At 19 weeks' gestation, the respiratory acinus is simple with only two or …

Ciliated Cells Of The Trachea Of The Rabbit, Treated With Cis-Diamminedichloroplatinum (Ii) Alone, Or In Combination With Ionizing Radiation, M. Albertsson, C. H. Hakansson, C. Mercke

Scanning Electron Microscopy

The ciliated epithelium of the rabbit trachea was irradiated with daily fractions of 2 Gy to an accumulated dose of 20 Gy (TD: 2, 6, 10, 16, or 20 Gy). Fifteen to forty-five minutes before start of the first irradiation ( treatment day 1) , 5 mg cis-DDP was given by intraperitoneal injection to each rabbit. Examination was made 1-10 days after each fractionation schedule, when specimens were ta ken for investigations.

Scanning electron microscope investigations showed a gradual development of ciliary damage from blebs on the cilia to swollen tips, broken and bent cilia and finally an epithelial lining …

The Migration Of Amphibian Primordial Germ Cells In The Chick Embryo, Marjorie A. England, Alma P. Swan, P. Dane

Scanning Electron Microscopy

A fibrous band of extracellular materials on the chick embryo area pellucida/area opaca border is a preferential migratory pathway for chick embryo primordial germ cells (PGC). This band contains fibronectin, collagen Type I and sulphated glycosaminoglycans. It is known that PGCs from Xenopus laevis interact with fibronectin as they undergo migration in the embryo from their site of origin to the gonads. To establish whether this pathway is species specific in chick embryos it was decided to transplant PGC from Xenopus laevis embryos stage 48 on to chick embryos stage 4 fibrous band. Their rapid migration on this extracellular matrix …

Utah Science Vol. 47 No. 2, Summer 1986

Utah Science

30 "CONSIDER THE LILIES OF THE FIELD ... " R. J. Shaw and M. C. Williams A review of the distribution. attributes. and hazards of the naturally occurring Iiles in Utah.

36 IMPROVING PROFITS FOR UTAH DAIRY FARMS: A COMPUTER SIMULATION OF MANAGEMENT PRACTICES D. Bailey. J. c. Andersen and G. L. Helms Economists say feeding balanced rations. altering the crop mix. and herd expansion could help increase profits in dairying.

41 GLOBEMALLOWS: FORBS FOR UTAH RANGELANDS B. M. Pendery and M. D. Rumbaugh Gooseberry-leaf globemallow and Munro globe mallow and Munro globemallow show potential as forages for Utah's rangelands. …

Correlative Scanning Electron Microscopy In The Study Of Human Gastric Mucosa, F. Bonvicini, M. C. Maltarello, P. Versura, D. Bianchi, G. Gasbarrini, R. Laschi

Scanning Electron Microscopy

We studied two aspects of the human gastric mucosa: - the surface morphology of mucous cells, as viewed by scanning electron microscopy (SEM); - the glycosidic components of intracellular mucins, characterized by means of lectins. The latter were conjugated with fluorescein isothiocyanate and with colloidal gold-silver for the visualization of the reaction products in light microscopy (LM) and in SEM (backscattered mode) respectively.

The surface morphology of mucous cells appears to be correlated to the secretory state.In gastric ulcers we found a prevalence of non-secreting cells. A decrease in glycosidic receptors for fucose-binding lectin and galactose-(l-3)-N-acetyl-galactosamine-binding lectin was also observed. …

Intrauterine Device (Iud) Associated Pathology: A Review Of Pathogenic Mechanisms, Waldemar A. Schmidt, Karmen L. Schmidt

Scanning Electron Microscopy

This paper summarizes our studies of IUD-related disease with those previously published by others. Our data are based upon 51 IUDs and 42 index cases of IUD-related disease demonstrating specific processes. Gross, dissecting microscope, scanning electron microscope and X-ray microanalysis examinations were made of selected IUDs and associated tissues. Tissue associated with the IUDs revealed inflammation in 59.4%, calcific material in 6.3% and no abnormality in 34.4%. JUD-associated tissue responses were accompanied by changes of the IUD; these changes involved deposition of substances upon the IUD surface and degradation of the JUD itself. Disintegration of the IUD, its string or …

Scanning Electron Microscopy Of The Microvascular System In The Inner Ear, Yoshiaki Nakai, Haruhiko Masutani, Hiromasa Cho

Scanning Electron Microscopy

In the present work, vessels casts in the inner ear of the rat and guinea pig, prepared by casting method using Mercox resin, were subjected to scanning electron microscopic examination and following results were obtained: 1) In adult guinea pig, numerous capillary nets were found in the following parts: stria vascularis, spiral ligament, spiral prominence, Corti's organ, spiral ganglion, plexus cochlearis, semicircular ampulla, saccule, utricle, and endolymphatic sac. These were consistent with functionally and morophologically important areas in the inner ear. 2) In the central side of the area with capillary nets, arterioles were found to run throughout, like a …

Scanning And Transmission Electron Microscopic Study Of Recovered Porcine Aortic Valved Conduits, D. J. Allen, I. H. Fentie, J. T. Davis, Angela Lineen

Scanning Electron Microscopy

Four explanted porcine aortic valved conduits were examined using scanning and transmission electron microscopy. Sources of obstruction such as neointima or "peel" and calcification were observed. In one sample the neointima was found to possess an unusually large expanse of squamous cells partially lining the luminal surface. This lining much resembled a normal endothelium, which is not an expected feature of neointima. Cells, presumably of host origin, were noted upon the leaflet surfaces. They did not seem as well organized as those found on the neointima. Calcification did not seem greatly advanced but was clearly apparent. Certain treatments proposed by …

Statoconia Formation In Molluscan Statocysts, Michael L. Wiederhold, Christine E. Sheridan, Nancy K. R. Smith

Scanning Electron Microscopy

The gravity sensors of all molluscs phylogenetically below the cephalopods are spherical organs called statocysts. The wall of the sphere contains mechanosensory cells whose sensory cilia project into the lumen of the cyst. The lumen is filled with fluid and dense "stones", the statoconia or statoliths, which sink under the influence of gravity to load, and stimulate, those receptor cells which are at the bottom. The statuconia of Aplysia californica are shown to be calcified about a lamellar arrangement of membranes. Similar lamellar membrane arrangements are seen within the receptor cells, and their possible role in the formation of the …

Cells From Xenopus Laevis Gastrulae Adhere To Fibronectin-Sepharose Beads And Other Lectin Coated Beads, Kurt E. Johnson, Michael H. Silver

Scanning Electron Microscopy

Cells from Xenopus laevis blastulae have a poorly developed ability to adhere to Sepharose beads covalently coupled to bovine plasma fibronectin (FN-beads). They do, however, have the ability to adhere to con A-beads and cytodex-1 and cytodex-3 beads. Beginning at the early gastrula stage, there is a progressively increasing ability of cells to adhere to FN-beads. Gastrula cells adhere to FN-beads by the formation of large ruffling lamellipodia. These cells can translocate on the surface of FN-beads; and when attached to both beads and the surrounding glass substratum of culture vessels, have the ability to move the beads extensively. Gastrula …

Adverse Effects Of Metals On The Alveolar Part Of The Lung, Anne Johansson, Per Camner

Scanning Electron Microscopy

Rabbits were exposed to low levels of metal dust or metal ions by inhalation for 1-8 months, 5 days/week and 6h/day. Following exposure lung tissue was examined by light and electron microscopy, the lung content of phospholipid was analyzed and the morphology and function of alveolar macrophages were investigated.

Metallic nickel dust as well as soluble nickel chloride produced accumulation of macrophages and laminated structures in alveoli and increased volume density of alveolar type II cells. The amount of phospholipids was elevated, mainly due to an increase in disaturated phosphatidylcholine. After one month exposure to metallic nickel dust or soluble …