Digital Commons Network^™

Microbial Dark Matter: Culturing The Uncultured In Search Of Novel Chemotaxonomy, Sarah J. Kennedy

USF Tampa Graduate Theses and Dissertations

Although <1% of environmental isolates can be cultured, insights into the culturable fraction refine future techniques to unlock the cryptic biodiversity. This cryptic biodiversity is omnipresent within culture-independent surveys of environmental bacterial populations. Subsequently, these elusive organisms are termed “microbial dark matter” due to the intrigue surrounding their potential taxonomic and biosynthetic impacts. To this end of investigating bacterial-specific dark matter, we employed high-throughput culturing techniques to examine Gulf of Mexico sediments and sponges for chemotaxonomically important bacteria. Through stepwise implementation of permissive and selective recovery techniques, we succeeded in recovering over 150 unique bacterial isolates. We observed several isolates that pose as chemotaxonomically interesting, including several strains that belong to critical bioremediation taxa. In addition to these biotechnological-amenable isolates, we have also succeeded in culturing taxa historically attributed with antibacterial discoveries. One such isolate was a previously sponge-associated organism belonging to the rarely cultured Verrucosispora genus. Through extensive comparative genomics, we identified it as a novel species and subsequently named it Verrucosispora sioxanthis sp. nov. with the published genome serving as a resource for continued microbial dark matter investigations. Following continued passaging of the organism, we observed improved growth, biomass, and liquid growth dispersal within each increasingly sub-passaged generation of the isolate. As such, the laboratory attenuation, or domestication, of the organism warranted in-depth whole genomic analyses to generate insight into the increased laboratory sustainability. Through phenotypically-guided bioinformatics, we determined that the domestication was mediated through genome-wide selective pseudo- or non-pseudogenization of genes. As the previously sponge-associated organism evolved with the selection pressures of the static laboratory, we demonstrate that isolate effectively underwent genomic atrophy to resolve into a largely stable, sustainable laboratory-domesticated strain. Collectively, our chemotaxonomic discovery pipeline expands on the culturable bacterial biodiversity and ultimately provides a previously uncultured model organism for initial forays into resolving the microbial dark matter knowledge gap.

Learning, Planning For, And Reflecting On Teaching Mathematics For Social Justice: A Case Study Investigation Of A High School Mathematics Methods Course, Queshonda J. Kudaisi

USF Tampa Graduate Theses and Dissertations

While much is known about learning to teach for social justice in other content areas, less is known about how mathematics education students learn to teach mathematics for social justice, specifically at the high school level. Hence, this mixed-methods convergent case study addresses this gap in the research literature by examining and describing the experiences of mathematics education students learning to teach mathematics for social justice within the context of a high school mathematics methods course at a large research university in the southeastern United States. The data sources accessed for this study include a survey measuring beliefs about teaching …

Online Perceptions Of Panamanian Prisons And Incarcerated Persons: An Analysis Of Youtube User Comments, Mahaleth J. Sotelo

USF Tampa Graduate Theses and Dissertations

The purpose of this study is to explore and understand the frameworks in which prisons and incarcerated persons are discussed amongst commenters under YouTube videos displaying media on Panamanian prisons. The study incorporates a mixed methods approach by conducting a general content analysis of YouTuber comments to address themes within the discussion. Additionally, these themes were quantified and modeled using predictive variables collected such as number of comment likes, number of comment dislikes, and number of comment replies, alias type (screen name or name-like), presence of profile picture, and profile picture type. The themes found were 1) punitive, 2) justifying …

Sanitation Marketing In Uganda: The Impact Of Gender On Participation And Outcomes, Elizabeth Vicario

USF Tampa Graduate Theses and Dissertations

Uganda aims to reach universal access to basic sanitation under Sustainable Development Goal #6, but achieving this target by 2030 would require doubling the annual rate of progress. Women are disproportionately affected by poor sanitation due to their health needs and risk of facing violence, but they have historically been excluded from sanitation planning. Low household demand and poor service delivery have stalled progress in Uganda. Market-based sanitation (MBS) is a novel method of developing a sanitation market through stimulating demand and supporting a competitive atmosphere for product and service providers. This research studies the sanitation marketing activities implemented by …

A Functional Characterization Of The Omega (Ω) Subunit Of Rna Polymerase In Staphylococcus Aureus, Shrushti B. Patil

USF Tampa Graduate Theses and Dissertations

In bacteria, RNA polymerase (RNAP) is a well-characterized and highly conserved multi-subunit enzyme complex responsible for transcription of DNA into RNA. The ω subunit (rpoZ/RpoZ), one of the smaller accessory subunits of RNAP, is often overlooked and under-studied, however, particularly in Gram-positive species. We have previously shown that deleting ω impacts the integrity of RNA polymerase in S. aureus, especially the β' subunit, and alters preference of the core enzyme for sigma factors, skewing heavily towards σB instead of the housekeeping σ factor. Consequently, this causes deregulation of myriad transcriptional processes, strongly rewiring gene expression circuits, and ultimately impairing the …

Mobile Response Teams And The Youth Emergency Behavioral Health System, Paige J. Alitz

USF Tampa Graduate Theses and Dissertations

Introduction: This dissertation includes three separate manuscripts that coalesce under the shared topic of mobile crisis response and the emergency behavioral health system. Method: The first manuscript includes a synthesis of the research on mobile crisis response from the 1960s to present day to assess whether mobile crisis services can improve mental health care access in the U.S. for youth and adults. The second manuscript includes bivariate and multivariate analyses of MRT participant data to characterize participants who receive an involuntary psychiatric evaluation versus those who do not, and to assess factors associated with involuntary psychiatric evaluation or referral to …

Application Of Gold Nanoshells For Photothermal Therapy In Lung Cancer Cells, Yva K. Luc

USF Tampa Graduate Theses and Dissertations

Lung cancer is the leading cause of cancer deaths in the world. For the year 2020, it was estimated 1.8 million deaths were due to lung cancer alone, making it more lethal than breast cancer and prostate cancer combined. For the year 2021, it is estimated 235,760 new cases of lung cancer and 50% will lead to death. The primary cause of lung cancer is due to cigarette smoking, which accounts for 80% of all lung cancer deaths. Lung cancer screening relies on only different methods, biopsy, bronchoscopy, ultrasound, chest x-ray film, and sputum cytology. These techniques are used to …

Investigation Of An Alternative Protocol For The Production Of Sars-Cov-2 Antigenic Proteins, Nichole Ninaltowski

USF Tampa Graduate Theses and Dissertations

With the COVID-19 pandemic showing no signs of slowing down, large-scale antigenic protein production is still needed for surveillance using serologic assays. From screening to vaccines to biotherapeutics, being able to produce the proteins for these assays is essential; however, the current gold standard method for producing SARS-CoV-2 spike proteins is prohibitively expensive for most research groups.

Alternative methods of transfecting mammalian cells to produce recombinant proteins that are relatively inexpensive have been used for years. Unlike the expensive, commercially available lipid-based methods, other established methods such as polyethyleneimine (PEI), are considerably easier, and cheaper to meet the needs of …

Community Assessment Of Water Perceptions And Household Point-Of-Use Treatment Methods In Madagascar, Isabella Rose Silverman

USF Tampa Graduate Theses and Dissertations

One in four people around the world do not have access to safe drinking water, which means that two billion people globally lack access. In high-income countries, 0.02% of yearly deaths are attributed to unsafe water sources; however, an alarming 10.6% of deaths occur in Madagascar, for the same reason.

To achieve Sustainable Development Goal 6: Ensure access to water and sanitation for all, a collaboration between public health and engineering is critical. Within the public health sector, social marketing is commonly utilized to address a problem by using commercial marketing principles to change behavior for the good of society. …

Laboratory Examination Of Lead Weights Harvested From Pitcher Pumps In Eastern Madagascar, Madelyn Wilson

USF Tampa Graduate Theses and Dissertations

Previous sampling campaigns in Tamatave, Madagascar, conducted by the University of South Florida (USF) have identified lead contamination in drinking water sourced from self-supply pitcher pumps. The pitcher pump piston and foot valves were identified as the primary source of lead contamination since they are often manufactured from recycled lead acid batteries. The previous field sampling technique consisted of collecting an aliquot of a 10-15-liter composite sample from the pitcher pump for dissolved lead analysis; however, this technique (1) neglects the contribution of particulate lead, (2) does not properly account for the effects of water stagnation inside the pitcher pump …

Online And Adjusted Human Activities Recognition With Statistical Learning, Yanjia Zhang

USF Tampa Graduate Theses and Dissertations

Wearable human activity recognition (HAR) is a widely application system for our daily life. It hasbeen built in many devices, such as smartphone, smartwatch, activity tracker, and health monitor. Many researchers try to develop a system which requires less memory space and power, but has fast and accurate classification results. Moreover, the objective of adjusting the classifier by the system self is also a study direction. In the present study, we introduced the machine learning methods to both smartphone data and smartwatch data and an adjusted model with the continuous generating data. Further, we also proposed a new HAR system …

The Weekly Challenger, October 28, 2021, The Weekly Challenger, Et Al

Weekly Challenger Newspaper Collection

The October 28, 2021, issue of The Weekly Challenger.

Development Of Small-Scale Power Supplies For Wearable Medical Diagnostic Devices, Donny Stiner

USF Tampa Graduate Theses and Dissertations

As devices continue the trend of becoming increasingly smaller in scale to allowfor wearability, the power supply modules driving these technologies will in turn become reduced in size as well. This progression will call for advanced techniques to fabricate power supplies on wearable device platforms which still permit the circuit to operate effectively. One such crucial technique, which is discussed in this thesis, is the implementation of power saving measures to increase battery lifespan on wearable devices.

Equipment found in the medical field today can be quite bulky and requireinconvenient means of transportation and take up an excessive amount of …

Quercetin Loaded Nanoparticles For The Treatment Of Dry Eye Disease, Bassma Sami Nashaat Hamed Eltanameli

USF Tampa Graduate Theses and Dissertations

Dry eye disease (DED) is a common condition that affects the eye's anterior surface due to tear deficiency or excessive evaporation. The lack of tears leads to dryness and initiation of an inflammatory response which can irritate the eye and interfere with daily activities. Current treatment depends mainly on artificial tears eye drops which offer temporary relief and require repetitive administration due to frequent blinking. Prescription medications are also available but need around three months to relieve dry eye symptoms. Quercetin is a flavonoid that has an anti-inflammatory and antioxidant effect. However, it suffers from low water solubility and low …

Evaluating Daily Systemic Betaine Delivery In Alleviating Dry-Amd Pathophysiology, Dawin Khiev

USF Tampa Graduate Theses and Dissertations

MnSod (Sod2) deletion in the retinal pigment epithelium (RPE) of Sod2flox/floxVMD2-cre mice leads to oxidative stress-induced apoptosis of the RPE and photoreceptor cells that show salient features of dry-AMD. The purpose of this study was to investigate the potential antioxidant effect of betaine in protecting retinal health in Sod2flox/floxVMD2-cre mice. Betaine low dose (50mg/Kg Body Weight) and high dose (500mg/kg Body Weight, n=9) were administered intraperitoneally to 8-weeks old male and female Sod2flox/floxVMD2-cre mice daily for four months, while the control group (Sod2flox/floxVMD2-cre mice; n=9) received saline. We used spectral-domain optical coherence tomography (SD-OCT), retinal outer nuclear layer (ONL) thickness …

Seasonality In Competence To Transmit West Nile Virus For A Widespread Reservoir, Kyle L. Koller

USF Tampa Graduate Theses and Dissertations

All organisms face a central conflict; a limited supply of resources must beoptimally allocated to competing biological functions. Utilizing robust immune defenses can be energetically costly;, thus, immune function is often diminished when other processes, such as reproduction, molt, or migration demand energy. As wild animals residing in temporally dynamic environments face seasonal patterns in resource availability, infectious disease risk, and environmental suitability, many species have evolved endogenous biological rhythms so that the performance of reproduction, molt, or migration and the associated trade-off with immune function be completed when doing so best promotes fitness. In turn, this demand to optimize …

The Media Reproduction Of Racial Violence: A Content Analysis Of News Coverage Following The Death Of George Floyd Jr., Keylon Lovett

USF Tampa Graduate Theses and Dissertations

The media has played a critical role in reproducing anti-Black violence in the United States, which has often harmed African American communities. Historically, the white press has depicted graphic imagery and descriptions of Black people being brutalized, with little ethical regard to their harmful effects. The Black press has historically challenged negative portrayals in the white media and shown more nuance, to protect the Black audience it represents. This dynamic underpins media depictions of racial violence still seen today. Darnella Frazier’s video capture of George Floyd’s death by Minneapolis police, was widely shared in the weeks following the incident, across …

Multi-Material Hybrid Additive And Subtractive Manufacturing Of Photonic Devices, Roger Brandon Tipton

USF Tampa Graduate Theses and Dissertations

Optoelectronics focuses on the application of electronic devices that utilize forms of electromagnetic radiation including visible light and other wavelengths including ultraviolet and infrared. Optoelectronics typically include devices that emit light and detect light. Devices created using this technology include light-emitting diodes, laser diodes, photoresistors, photodiodes, solar cells, phototransistors, and fiber optics. Fiber optic interconnects are interesting devices in that they are used in conjunction with optoelectronic devices to transmit information between individual optoelectronic components.

Optoelectronics are typically produced by traditional lithography methods including etching, writing, and printing on rigid silicon substrates for fabricating devices. Other techniques like glass or …

Design Of Acoustic Wave Sensor System For Detection Of Btex In Gas Phase, Veselinka Mitevska

USF Tampa Graduate Theses and Dissertations

Benzene, toluene, ethylbenzene, and xylene are classified as volatile organic compounds (VOC), which if present at higher concentrations can cause acute and/or chronic health effects. To protect people from VOCs, the occupational safety and health agency (OSHA) established short term and eight-hour workday exposure limits. The short time levels of exposure for BTEX materials are 5 ppm, 150 ppm, 125 ppm, and 150 ppm, for benzene, toluene, ethylbenzene, and xylene respectively. The eight-hour workday exposure for these same materials are 1 ppm for benzene, 200 ppm for toluene, 100 ppm for ethylbenzene and 100 ppm for xylene. Although there are …

Driving Health Care Results With Authenticity, Kindness, And Vulnerability: A New Model Of Authentic Leadership, John D. Couris

USF Tampa Graduate Theses and Dissertations

Current management scholars have defined authentic leadership as consisting of four dimensions: relational transparency, self-awareness, balanced processing, and internalized moral perspective. This research extends the concept of authentic leadership to include kindness and vulnerability. I created two scales to measure kindness and vulnerability within the context of leadership and used them to examine perceptions of authentic leadership and its impact on three organizational outcomes: trust in one’s leader, work engagement, and psychological safety. Using a two-group, pretest-posttest experimental design, 44 Tampa General Hospital managers were either selected to participate in a four-hour authentic leadership training that included instruction on kindness …

Ay 2021/2022 Fs Meeting Minutes, 2021-10-27, Faculty Senate

Faculty Senate Archives

No abstract provided.

Modeling The Ambient Conditions Of A Manufacturing Environment Using Computational Fluid Dynamics (Cfd), Yang Liu

USF Tampa Graduate Theses and Dissertations

As manufacturing equipment evolve to higher speed and require high precision operations, the impact of environmental changes on machine accuracy becomes critical. Due to thermal expansion, the structure of the machine can change when ambient temperature varies. When the airflow in the laboratory changes, this also alters the operator's thermal comfort. Either the change in machine structure or operator comfort can ultimately affect machine accuracy. The manufacturing industry is currently using heating, ventilation, and air-conditioning (HVAC) systems to regulate the temperature of the working environment. However, since conventional HVAC systems determine whether to activate the HVAC system by collecting the …

The Multifaceted Role Of Ccar-1 In The Alternative Splicing And Germline Regulation In Caenorhabditis Elegans, Doreen Ikhuva Lugano

USF Tampa Graduate Theses and Dissertations

The Cell Division Cycle and Apoptosis Regulator (CCAR) family members are an enigmatic family of proteins regulating metabolism, cancer, apoptosis, DNA damage, and stress. Mammals have CCAR family members, CCAR1 and CCAR2/DBC1, which evolved from the founding family member CCAR-1/LST-3 expressed in Caenorhabditis elegans. Several studies have shown the importance of understanding these proteins' function in standard and altered physiological processes. Our studies aim to understand the genome-wide alternative splicing and germline regulation of Caenorhabditis elegans CCAR-1 in normal and heat shock conditions. Recently, mammalian CCAR family member CCAR2/DBC1 regulates the alternative splicing by forming a complex with ZNF326. This …

Effects Of Nitrate On Arsenic Mobilization During Aquifer Storage And Recovery, Hania Hawasli

USF Tampa Graduate Theses and Dissertations

Aquifer storage and recovery technology is used to sustain water resources and to prevent saltwater intrusion. The injected water can come from various resources, including treated wastewater. In pilot ASR studies in the Tampa Bay region, researchers found high As concentrations in the recovered water from the oxidation of the arsenopyrite that is embedded in the aquifers. The presence of dissolved O2 in the injected water is a major factor in the arsenopyrite oxidation during ASR, however the effects of NO3- on the arsenopyrite has not been studied yet. This is an important knowledge gap because injected water may contain …

Criminalizing Lgbtq+ Jamaicans: Social, Legal, And Colonial Influences On Homophobic Policy, Zoe C. Knowles

USF Tampa Graduate Theses and Dissertations

Based on colonial and neocolonial models of oppression, Jamaica has adopted many laws, policies, and systems mandated by the British monarchy. Many of these laws contain anti-LGBTQ+ policies which remain in effect today. To address the criminalization of LGBTQ+ identities, I used queer theory and queer criminology to analyse the ways Jamaica constructs LGBTQ+ people as criminals and how they are treated in the legal and criminal justice systems from a postcolonial standpoint. Using a qualitative text-based feminist and queer policy analysis, I investigated social, legal, and colonial influences on current anti-LGBTQ+ policy by looking at the Offences Against the …

An Assessment Of Nutrient Improvement In Surface Water Due To The Conversion Of Onsite Sewage Treatment And Disposal Systems To Sewerage, Jenelle A. Mohammed

USF Tampa Graduate Theses and Dissertations

Florida represents approximately 12% of all onsite sewage treatment and disposal systems (OSTDS) in the United States. Properly designed, constructed and maintained OSTDS are effective eco- friendly alternatives for the treatment of domestic wastewater from households not serviced by public sewer systems. However, conventional OSTDS are not designed to effectively remove nitrogen. If installed and improperly used, they may contribute other pollutants. OSTDS have also been linked to degraded water quality in some regions. However, it has been difficult to quantify OSTDS impacts on water quality because of various confounding factors such as point and non-point sources of nutrient input. …

Multi-Object Localization In Robotic Hand, Tsing Tsow

USF Tampa Graduate Theses and Dissertations

We have developed a machine learning approach to localized objects inside a robotic hand using only images from 2D cameras. Specifically, we used deep learning method (You Only Look Once, YOLO) and Iterative closest Point (ICP) to estimate the 3D coordinates of the objects in a robotic hand. This method will also output the number of objects inside the robotic hand in addition to the coordinates of the objects. We have demonstrated the performance with simulation and obtained typical accuracy within a few pixels (couple mm) and counting accuracy of about 76%. We have also applied it to real images, …

Residential Curbside Recycle Context Analysis, Ntchanang Mpafe

USF Tampa Graduate Theses and Dissertations

Curbside recycling as a preferred mode of residential and municipal sustainability goals seems to have an overwhelming acceptance and adoption in the US. About 69.8 million out of 97.3 million (72%) single-family households in the United States have access to curbside recycling services (State of Curbside Recycling Report, 2020). Collectively, the programs divert about nine million tons of recyclables from landfill disposal each year (Cottom, 2019).

For a design that started in the 1980s in the US, its rapid universal adoption seems to have precluded a concerted effort in examining the coproduced nature (Households: service receptors and Municipalities: service providers) …

Teachers' Perspectives Of Implementing Restorative Justice, Antonio G. Smith

USF Tampa Graduate Theses and Dissertations

The use of restorative justice in the school system is an emerging philosophy for handling student discipline that seeks alternative methods for solving problematic behavior than traditional or retributive approaches that are detrimental to the education and well-being of students. Student discipline is a controversial topic in education circles, resulting in many school districts looking to implement restorative practice program initiatives. Responses that are restorative, largely decrease the chances of recidivism. Zero-tolerance policies that were originally enforced to keep schools safe, have proven to not have the desired effect as intended, most arguably making schools less safe. There is a …

Experiences Of Saudi Arabian Mothers Of Young Children With Disabilities: An Exploratory Study, Samirah Bahkali

USF Tampa Graduate Theses and Dissertations

Saudi Arabia is one of the countries that seeks to provide and develop services for children with disabilities and their families. As times have progressed, regulations and laws have placed an emphasis on providing suitable and better services for children with disabilities and their families. However, a gap exists in the literature regarding the overall experiences of Saudi Arabian mothers of children with disabilities in the early childhood years from birth to age ten (Al Otaibi & Al Sartawi, 2009; Alazemi, 2010). This study explored the experiences of Saudi Arabian mothers of children with disabilities. A qualitative interview approach, utilizing …