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Is Outdoor Recreation Associated With Greater Climate Change Concern In The United States?, Kyle W. Knight, Feng Hao Jan 2022

Is Outdoor Recreation Associated With Greater Climate Change Concern In The United States?, Kyle W. Knight, Feng Hao

Sociology Sarasota Manatee Campus Faculty Publications

There has been extensive research on the association between environmental attitudes and outdoor recreation (or nature-based leisure activities) since the 1970s. There is now considerable evidence to support the claim that spending time in nature leads to greater connectedness to nature and thereby greater pro-environmental attitudes and behavior. However, there is an absence of research focused specifically on the association between outdoor recreation and concern for climate change, which is arguably the most pressing environmental problem facing the world today. We build on previous research by using the 2021 General Social Survey and structural equation modeling to analyze the association …

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Enacting Economic Resilience: A Synthesis Of Economic And Communication Frameworks, Timothy Betts, Patrice M. Buzzanell Jan 2022

Enacting Economic Resilience: A Synthesis Of Economic And Communication Frameworks, Timothy Betts, Patrice M. Buzzanell

Communication Faculty Publications

This work examines three frameworks for responding to economic disruption: risk mitigation, systemic recovery, and economic resilience. Specifically, by reviewing the metatheoretical commitments, analytic contexts, and implications of two economic perspectives, represented by risk mitigation and systemic recovery, we argue that current approaches to understanding resilience in academic economics have failed to address ongoing and emergent disruptions in the economic and social world. In response, this work also reviews a possible synthesis of economic and communication frameworks. This review places the economic resilience framework, inspired by the communication theory of resilience, in conversation with extant literature in economics, communication studies, …

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Towards A “Digital” Sports Journalism: Field Theory, Changing Boundaries And Evolving Technologies, Gregory P. Perreault, Travis R. Bell Jan 2022

Towards A “Digital” Sports Journalism: Field Theory, Changing Boundaries And Evolving Technologies, Gregory P. Perreault, Travis R. Bell

School of Advertising & Mass Communications Faculty Publications

Long trivialized as the “toy department,” sports journalism nevertheless represents an enduring, and vital subfield within journalism. As with many niches of journalism, sports journalism has needed to adjust to changes resulting from the technology of the field. In particular, digital sports journalism faces pressure from adjacent fields, represented in team and player media, which perform many of the same tasks historically attributed to sports journalism. Through the lens of field theory, the present study reports on long-form interviews with 47 sports journalists who self-defined their work as digital journalism. This study argues that the perception of insurgents—team media that …

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Media And Mass Shootings: Field Theory In Cnn News Coverage Of The Columbine High School And Parkland High School Mass Shootings, Hannah Hume, Gregory P. Perreault Jan 2022

Media And Mass Shootings: Field Theory In Cnn News Coverage Of The Columbine High School And Parkland High School Mass Shootings, Hannah Hume, Gregory P. Perreault

School of Advertising & Mass Communications Faculty Publications

Through discourse analysis, this article seeks to compare the cable news coverage of the Columbine High School shooting and the Marjory Stoneman Douglas High School shooting (n = 81) in the first two days of coverage using field theory, with CNN broadcast transcripts as the unit of analysis. The research showed that the shooter was the dominant shaping force in the CNN discourse in both school shooting events.

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Discourse Of Gaming: A Conceptual Framework Of Gaming As An Interpretive Community, Gregory P. Perreault, Teresa Lynch Jan 2022

Discourse Of Gaming: A Conceptual Framework Of Gaming As An Interpretive Community, Gregory P. Perreault, Teresa Lynch

School of Advertising & Mass Communications Faculty Publications

Situating gaming as a cultural practice aimed at con-structing issues of power through cultural discussions necessitates a framework of discourse of gaming to explain how meanings around gaming practice develop. Built on the premise that gaming offers mul-timodal opportunities for interaction, we introduce the discourse of gaming framework to connect pro-cesses of boundary work, definition making, and legit-imization to the activities of gaming, the gamer’s identity, and the consequences of gaming. Through a survey of Reddit gaming fan communities, discourse of gaming here reflects the power perceived outsiders (e.g., journalists, academics, and politicians) have on shaping the discourse.

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The Lifestyle Of Lifestyle Journalism: How Reporters Discursively Manage Their Aspirations In Their Daily Work, Gregory P. Perreault, Valerie Harlow Belair-Gagnon Jan 2022

The Lifestyle Of Lifestyle Journalism: How Reporters Discursively Manage Their Aspirations In Their Daily Work, Gregory P. Perreault, Valerie Harlow Belair-Gagnon

School of Advertising & Mass Communications Faculty Publications

This study seeks to explore the motivations and labor of lifestyle, or “soft news,” journalists. Rooted in the lens of discursive institutionalism and through 30 interviews with lifestyle journalists in the United States, this study reflects on the aspirational labor—the opportunity to “do what you love”—that motivates entry into journalism but also can encourage disengagement. This study finds that while lifestyle journalists are motivated to enter the profession because of their own personal connection to the topic, their desire to be embedded in the topic and their love for the people in the genre, they also have to negotiate institutional …

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Freshwater Macrophytes Harbor Viruses Representing All Five Major Phyla Of The Rna Viral Kingdom Orthornavirae, Karyna Rosario, Noémi Van Bogaert, Natalia B. López-Figueroa, Haris Paliogiannis, Mason Kerr, Mya Breitbart Jan 2022

Freshwater Macrophytes Harbor Viruses Representing All Five Major Phyla Of The Rna Viral Kingdom Orthornavirae, Karyna Rosario, Noémi Van Bogaert, Natalia B. López-Figueroa, Haris Paliogiannis, Mason Kerr, Mya Breitbart

Marine Science Faculty Publications

Research on aquatic plant viruses is lagging behind that of their terrestrial counterparts. To address this knowledge gap, here we identified viruses associated with freshwater macrophytes, a taxonomically diverse group of aquatic phototrophs that are visible with the naked eye. We surveyed pooled macrophyte samples collected at four spring sites in Florida, USA through next generation sequencing of RNA extracted from purified viral particles. Sequencing efforts resulted in the detection of 156 freshwater macrophyte associated (FMA) viral contigs, 37 of which approximate complete genomes or segments. FMA viral contigs represent putative members from all five major phyla of the RNA …

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Applying The Age-Friendly-Health System 4m Paradigm To Reframe Climate-Related Disaster Preparedness For Nursing Home Populations, David Dosa, Dylan Jester, Lindsay Peterson, Debra Dobbs, Kathy Black, Lisa Brown Jan 2022

Applying The Age-Friendly-Health System 4m Paradigm To Reframe Climate-Related Disaster Preparedness For Nursing Home Populations, David Dosa, Dylan Jester, Lindsay Peterson, Debra Dobbs, Kathy Black, Lisa Brown

Aging Studies Sarasota Manatee Campus Faculty Publications

No abstract provided.

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Field Insurgency In Lifestyle Journalism: How Lifestyle Journalists Marginalize Instagram Influencers And Protect Their Autonomy, Gregory P. Perreault, Folker Hanusch Jan 2022

Field Insurgency In Lifestyle Journalism: How Lifestyle Journalists Marginalize Instagram Influencers And Protect Their Autonomy, Gregory P. Perreault, Folker Hanusch

School of Advertising & Mass Communications Faculty Publications

While Facebook and Twitter have received significant scholarly attention for their role in shaping the journalistic field, Instagram has received sparse attention in comparison. The present study examines how lifestyle journalists (n = 63) from Austria and the United States perceive Instagram influencers operating in relation to the journalistic field. Instagram influencers, empowered by the digital medium, would seem to be in direct competition with lifestyle journalists in terms of content. Through the theoretical lenses of boundary work and field, this study argues that lifestyle journalists—long relegated to the periphery of the journalistic field—discursively leverage the presence of influencers …

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When Journalists Are Voiceless: How Lifestyle Journalists Cover Hate And Mitigate Harassment, Gregory P. Perreault, Kaitlin Miller Jan 2022

When Journalists Are Voiceless: How Lifestyle Journalists Cover Hate And Mitigate Harassment, Gregory P. Perreault, Kaitlin Miller

School of Advertising & Mass Communications Faculty Publications

Often trivialized within the broader journalistic field, lifestyle journalists would seem to have the dream job: the opportunity to get paid to do what they love. The present study explores an under-discussed but material aspect of the job; namely, how lifestyle journalists undertake issues of hostility. Through the lens of the theory of hostility towards the press and in-depth interviews with lifestyle journalists (n = 24), this study argues that journalists tend to cover issues of hate against their audience members but seek to ignore harassment when directed at them.

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Covering Hate: Field Theory And Journalistic Role Conception In Reporting On White Nationalist Rallies, Gregory P. Perreault, Brett Johnson, Leslie Klein Jan 2022

Covering Hate: Field Theory And Journalistic Role Conception In Reporting On White Nationalist Rallies, Gregory P. Perreault, Brett Johnson, Leslie Klein

School of Advertising & Mass Communications Faculty Publications

In the United States, journalists covering white nationalist groups find themselves in an impossible situation: how do you cover the newsworthy rallies—and the concerns raised by the local community—without providing a platform for hate speech? The present study conducts in-depth, semi-structured interviews with 18 journalists who have covered white nationalist rallies. Through the lens of field theory, this study seeks to understand how journalists conceive of their role in such coverage, how they situate themselves within the field, and how they articulate the best practices for this challenging form of reporting. This study finds that white nationalist rallies presented a …

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“It’S Not Hate But … ”: Marginal Categories In Rural Journalism, Gregory P. Perreault, Ruth Moon, Jessica Fargen Walsh, Mildred F. Perreault Jan 2022

“It’S Not Hate But … ”: Marginal Categories In Rural Journalism, Gregory P. Perreault, Ruth Moon, Jessica Fargen Walsh, Mildred F. Perreault

School of Advertising & Mass Communications Faculty Publications

Journalists who cover rural areas in the United States say they are afraid to report on hate groups, and this fear is exacerbated by close community ties and limited resources among rural journalists. We examine the concept of “hate speech” as a boundary object, analyzing in-depth interviews with U.S. journalists reporting in rural communities (n = 33) to better understand how rural journalists report on hate. We find that rural journalists articulate a clear definition for hate speech but struggle to apply that definition to events within their communities, even as they articulate numerous forms of hate. Journalists often dismissed …

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What Does It Mean To Be A Female Character In “Indie” Game Storytelling? Narrative Framing And Humanization In Independently Developed Video Games, Mildred F. Perreault, Gregory P. Perreault, Andrea Suarez Jan 2022

What Does It Mean To Be A Female Character In “Indie” Game Storytelling? Narrative Framing And Humanization In Independently Developed Video Games, Mildred F. Perreault, Gregory P. Perreault, Andrea Suarez

School of Advertising & Mass Communications Faculty Publications

Video games have long held a spotty history in their narratives regarding women. Most research has examined large budget games and identified issues of simplification, oversexualization, and a general lack of agency among female characters. The present study explores the gaming niche of “indie”—or independent game developer—video games in their representations of women in particular with Never Alone, Gone Home, and Her Story. These games were released around the time frame of the GamerGate controversy—a controversy which drew attention to the treatment of women in gaming culture—and hence, the games are used to reflect on a potential shift in games …

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Metajournalistic Discourse As A Stabilizer Within The Journalistic Field: Journalistic Practice In The Covid-19 Pandemic, Gregory P. Perreault, Mildred F. Perreault, Phoebe Maares Jan 2022

Metajournalistic Discourse As A Stabilizer Within The Journalistic Field: Journalistic Practice In The Covid-19 Pandemic, Gregory P. Perreault, Mildred F. Perreault, Phoebe Maares

School of Advertising & Mass Communications Faculty Publications

The COVID-19 Pandemic created a two-fold challenge for journalists: first, the task of gathering and distributing information vital to the responses of the public, and second, the challenge of mitigating the complexities of the journalism field. The purpose of this study is to connect the theoretical frameworks of metajournalistic discourse and field theory, using the touch point of journalistic practice. Prior research has postulated that metajournalistic discourse operates as a stabilizing force in the journalistic field. Using the timely test of the COVID-19 pandemic, this study seeks to explore the discursive construction of journalistic practice during a pandemic through the …

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Associations And Disease–Disease Interactions Of Covid-19 With Congenital And Genetic Disorders: A Comprehensive Review, Altijana Hromić-Jahjefendić, Debmalya Barh, Cecília Horta Ramalho Pinto, Lucas Gabriel Rodrigues Gomes, Jéssica Lígia Picanço Machado, Oladapo Olawale Afolabi, Sandeep Tiwari, Alaa A.A. Aljabali, Murtaza M. Tambuwala, Ángel Serrano-Aroca, Elrashdy M. Redwan, Vladimir N. Uversky, Kenneth Lundstrom Jan 2022

Associations And Disease–Disease Interactions Of Covid-19 With Congenital And Genetic Disorders: A Comprehensive Review, Altijana Hromić-Jahjefendić, Debmalya Barh, Cecília Horta Ramalho Pinto, Lucas Gabriel Rodrigues Gomes, Jéssica Lígia Picanço Machado, Oladapo Olawale Afolabi, Sandeep Tiwari, Alaa A.A. Aljabali, Murtaza M. Tambuwala, Ángel Serrano-Aroca, Elrashdy M. Redwan, Vladimir N. Uversky, Kenneth Lundstrom

Molecular Medicine Faculty Publications

Since December 2019, the COVID-19 pandemic, which originated in Wuhan, China, has resulted in over six million deaths worldwide. Millions of people who survived this SARS-CoV-2 infection show a number of post-COVID complications. Although, the comorbid conditions and post-COVID complexities are to some extent well reviewed and known, the impact of COVID-19 on pre-existing congenital anomalies and genetic diseases are only documented in isolated case reports and case series, so far. In the present review, we analyzed the PubMed indexed literature published between December 2019 and January 2022 to understand this relationship from various points of view, such as susceptibility, …

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Bioactive Peptides: Synthesis, Sources, Applications, And Proposed Mechanisms Of Action, Mohsen Akbarian, Ali Khani, Sara Eghbalpour, Vladimir N. Uversky Jan 2022

Bioactive Peptides: Synthesis, Sources, Applications, And Proposed Mechanisms Of Action, Mohsen Akbarian, Ali Khani, Sara Eghbalpour, Vladimir N. Uversky

Molecular Medicine Faculty Publications

Bioactive peptides are a group of biological molecules that are normally buried in the structure of parent proteins and become active after the cleavage of the proteins. Another group of peptides is actively produced and found in many microorganisms and the body of organisms. Today, many groups of bioactive peptides have been marketed chemically or recombinantly. This article reviews the various production methods and sources of these important/ubiquitous and useful biomolecules. Their applications, such as antimicrobial, antihypertensive, antioxidant activities, blood-lipid-lowering effect, opioid role, antiobesity, ability to bind minerals, antidiabetic, and antiaging effects, will be explored. The types of pathways proposed …

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Erythropoietin Interacts With Specific S100 Proteins, Alexey S. Kazakov, Eugenia I. Deryusheva, Andrey S. Sokolov, Maria E. Permyakova, Ekaterina A. Litus, Victoria A. Rastrygina, Vladimir N. Uversky, Eugene A. Permyakov, Sergei E. Permyakov Jan 2022

Erythropoietin Interacts With Specific S100 Proteins, Alexey S. Kazakov, Eugenia I. Deryusheva, Andrey S. Sokolov, Maria E. Permyakova, Ekaterina A. Litus, Victoria A. Rastrygina, Vladimir N. Uversky, Eugene A. Permyakov, Sergei E. Permyakov

Molecular Medicine Faculty Publications

Erythropoietin (EPO) is a clinically significant four-helical cytokine, exhibiting erythropoietic, cytoprotective, immunomodulatory, and cancer-promoting activities. Despite vast knowledge on its signaling pathways and physiological effects, extracellular factors regulating EPO activity remain underexplored. Here we show by surface plasmon resonance spectroscopy, that among eighteen members of Ca2+-binding proteins of the S100 protein family studied, only S100A2, S100A6 and S100P proteins specifically recognize EPO with equilibrium dissociation constants ranging from 81 nM to 0.5 µM. The interactions occur exclusively under calcium excess. Bioinformatics analysis showed that the EPO-S100 interactions could be relevant to progression of neoplastic diseases, including cancer, and other diseases. …

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Arrangement Of Hydrogen Bonds In Aqueous Solutions Of Different Globular Proteins, Amber R. Titus, Pedro P. Madeira, Luisa A. Ferreira, Alexander I. Belgovskiy, Elizabeth K. Mann, Jay Adin Mann Jr., William V. Meyer, Anthony E. Smart, Vladimir N. Uversky, Boris Zaslavsky Jan 2022

Arrangement Of Hydrogen Bonds In Aqueous Solutions Of Different Globular Proteins, Amber R. Titus, Pedro P. Madeira, Luisa A. Ferreira, Alexander I. Belgovskiy, Elizabeth K. Mann, Jay Adin Mann Jr., William V. Meyer, Anthony E. Smart, Vladimir N. Uversky, Boris Zaslavsky

Molecular Medicine Faculty Publications

This work presents the first evidence that dissolved globular proteins change the arrangement of hydrogen bonds in water, with different proteins showing quantitatively different effects. Using ATR-FTIR (attenuated total reflection—Fourier transform infrared) spectroscopic analysis of OH-stretch bands, we obtain quantitative estimates of the relative amounts of the previously reported four subpopulations of water structures coexisting in a variety of aqueous solutions. Where solvatochromic dyes can measure the properties of solutions of non-ionic polymers, the results correlate well with ATR-FTIR measurements. In protein solutions to which solvatochromic dye probes cannot be applied, NMR (nuclear magnetic resonance) spectroscopy was used for the …

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Editorial: Intrinsically Disordered Proteins And Regions: The Challenge To The Structure-Function Relationship, Angelo Toto, Pietro Sormanni, Cristina Paissoni, Vladimir N. Uversky Jan 2022

Editorial: Intrinsically Disordered Proteins And Regions: The Challenge To The Structure-Function Relationship, Angelo Toto, Pietro Sormanni, Cristina Paissoni, Vladimir N. Uversky

Molecular Medicine Faculty Publications

No abstract provided.

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Biapss: A Comprehensive Physicochemical Analyzer Of Proteins Undergoing Liquid–Liquid Phase Separation, Aleksandra E. Badaczewska-Dawid, Vladimir N. Uversky, Davit A. Potoyan Jan 2022

Biapss: A Comprehensive Physicochemical Analyzer Of Proteins Undergoing Liquid–Liquid Phase Separation, Aleksandra E. Badaczewska-Dawid, Vladimir N. Uversky, Davit A. Potoyan

Molecular Medicine Faculty Publications

The liquid–liquid phase separation (LLPS) of biomolecules is a phenomenon which is nowadays recognized as the driving force for the biogenesis of numerous functional membraneless organelles and cellular bodies. The interplay between the protein primary sequence and phase separation remains poorly understood, despite intensive research. To uncover the sequence-encoded signals of protein capable of undergoing LLPS, we developed a novel web platform named BIAPSS (Bioinformatics Analysis of LLPS Sequences). This web server provides on-the-fly analysis, visualization, and interpretation of the physicochemical and structural features for the superset of curated LLPS proteins.

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Small Heat Shock Protein 22 Improves Cognition And Learning In The Tauopathic Brain, Santiago Rodriguez Ospina, Danielle M. Blazier, Marangelie Criado-Marrero, Lauren A. Gould, Niad T. Gebru, David Beaulieu-Abdelahad, Xinming Wang, Elizabeth Remily-Wood, Dale Chaput, Stanley Stevens Jr., Vladimir N. Uversky, Paula C. Bickford, Chad Anthony Dickey, Laura J. Blair Jan 2022

Small Heat Shock Protein 22 Improves Cognition And Learning In The Tauopathic Brain, Santiago Rodriguez Ospina, Danielle M. Blazier, Marangelie Criado-Marrero, Lauren A. Gould, Niad T. Gebru, David Beaulieu-Abdelahad, Xinming Wang, Elizabeth Remily-Wood, Dale Chaput, Stanley Stevens Jr., Vladimir N. Uversky, Paula C. Bickford, Chad Anthony Dickey, Laura J. Blair

Molecular Medicine Faculty Publications

The microtubule-associated protein tau pathologically accumulates and aggregates in Alzheimer’s disease (AD) and other tauopathies, leading to cognitive dysfunction and neuronal loss. Molecular chaperones, like small heat-shock proteins (sHsps), can help deter the accumulation of misfolded proteins, such as tau. Here, we tested the hypothesis that the overexpression of wild-type Hsp22 (wtHsp22) and its phosphomimetic (S24,57D) Hsp22 mutant (mtHsp22) could slow tau accumulation and preserve memory in a murine model of tauopathy, rTg4510. Our results show that Hsp22 protected against deficits in synaptic plasticity and cognition in the tauopathic brain. However, we did not detect a significant change in tau …

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Ibuprofen Favors Binding Of Amyloid-Β Peptide To Its Depot, Serum Albumin, Ekaterina A. Litus, Alexei S. Kazakov, Eugenia I. Deryusheva, Ekaterina L. Nemashkalova, Marina P. Shevelyova, Andrey V. Machulin, Aliya A. Nazipova, Maria E. Permyakova, Vladimir N. Uversky, Sergei E. Permyakov Jan 2022

Ibuprofen Favors Binding Of Amyloid-Β Peptide To Its Depot, Serum Albumin, Ekaterina A. Litus, Alexei S. Kazakov, Eugenia I. Deryusheva, Ekaterina L. Nemashkalova, Marina P. Shevelyova, Andrey V. Machulin, Aliya A. Nazipova, Maria E. Permyakova, Vladimir N. Uversky, Sergei E. Permyakov

Molecular Medicine Faculty Publications

The deposition of amyloid-β peptide (Aβ) in the brain is a critical event in the progression of Alzheimer’s disease (AD). This Aβ deposition could be prevented by directed enhancement of Aβ binding to its natural depot, human serum albumin (HSA). Previously, we revealed that specific endogenous ligands of HSA improve its affinity to monomeric Aβ. We show here that an exogenous HSA ligand, ibuprofen (IBU), exerts the analogous effect. Plasmon resonance spectroscopy data evidence that a therapeutic IBU level increases HSA affinity to monomeric Aβ40/Aβ42 by a factor of 3–5. Using thioflavin T fluorescence assay and transmission electron microcopy, we …

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Sars-Cov-2 Intermittent Virulence As A Result Of Natural Selection, Alberto Rubio-Casillas, Elrashdy M. Redwan, Vladimir N. Uversky Jan 2022

Sars-Cov-2 Intermittent Virulence As A Result Of Natural Selection, Alberto Rubio-Casillas, Elrashdy M. Redwan, Vladimir N. Uversky

Molecular Medicine Faculty Publications

For the first time in history, we have witnessed the origin and development of a pandemic. To handle the accelerated accumulation of viral mutations and to comprehend the virus’ evolutionary adaptation in humans, an unparalleled program of genetic sequencing and monitoring of SARS-CoV-2 variants has been undertaken. Several scientists have theorized that, with the Omicron surge producing a more contagious but less severe disease, the end of COVID-19 is near. However, by analyzing the behavior shown by this virus for 2 years, we have noted that pandemic viruses do not always show decreased virulence. Instead, it appears there is an …

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Interferon-Β Activity Is Affected By S100b Protein, Alexey S. Kazakov, Alexander D. Sofin, Nadezhda V. Avkhacheva, Eugenia I. Deryusheva, Victoria A. Rastrygina, Maria E. Permyakova, Vladimir N. Uversky, Eugene A. Permyakov, Sergei E. Permyakov Jan 2022

Interferon-Β Activity Is Affected By S100b Protein, Alexey S. Kazakov, Alexander D. Sofin, Nadezhda V. Avkhacheva, Eugenia I. Deryusheva, Victoria A. Rastrygina, Maria E. Permyakova, Vladimir N. Uversky, Eugene A. Permyakov, Sergei E. Permyakov

Molecular Medicine Faculty Publications

Interferon-β (IFN-β) is a pleiotropic cytokine secreted in response to various pathological conditions and is clinically used for therapy of multiple sclerosis. Its application for treatment of cancer, infections and pulmonary diseases is limited by incomplete understanding of regulatory mechanisms of its functioning. Recently, we reported that IFN-β activity is affected by interactions with S100A1, S100A4, S100A6, and S100P proteins, which are members of the S100 protein family of multifunctional Ca2+-binding proteins possessing cytokine-like activities (Int J Mol Sci. 2020;21(24):9473). Here we show that IFN-β interacts with one more representative of the S100 protein family, the S100B protein, involved in …

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Early Taurine Administration As A Means For Halting The Cytokine Storm Progression In Covid-19 Patients, Alberto Rubio-Casillas, Ramesh C. Gupta, Elrashdy M. Redwan, Vladimir N. Uversky, Raied A. Badierah Jan 2022

Early Taurine Administration As A Means For Halting The Cytokine Storm Progression In Covid-19 Patients, Alberto Rubio-Casillas, Ramesh C. Gupta, Elrashdy M. Redwan, Vladimir N. Uversky, Raied A. Badierah

Molecular Medicine Faculty Publications

Around the world, more than 6.2 million individuals have died as a result of coronavirus disease 2019 (COVID-19). According to a recent survey conducted among immunologists, epidemiologists, and virologists, this disease is expected to become endemic. This implies that the disease could have a continuous presence and/or normal frequency in the population. Pharmacological interventions to prevent infection, as well as to treat the patients at an early phase of illness to avoid hospitalization are essential additions to the vaccines. Taurine is known to inhibit the generation of all inflammatory mediators linked to the cytokine storm. It can also protect against …

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New Evidence Of The Importance Of Weak Interactions In The Formation Of Pml-Bodies, Alexander V. Fonin, Sergey A. Silonov, Anna S. Fefilova, Olesya V. Stepanenko, Anastasia A. Gavrilova, Alexey V. Petukhov, Anna E. Romanovich, Anna L. Modina, Tatiana S. Zueva, Evgeniy M. Nedelyaev, Nadejda M. Pleskach, Irina M. Kuznetsova, Vladimir N. Uversky, Konstantin K. Turoverov, Mirya L. Kuranova Jan 2022

New Evidence Of The Importance Of Weak Interactions In The Formation Of Pml-Bodies, Alexander V. Fonin, Sergey A. Silonov, Anna S. Fefilova, Olesya V. Stepanenko, Anastasia A. Gavrilova, Alexey V. Petukhov, Anna E. Romanovich, Anna L. Modina, Tatiana S. Zueva, Evgeniy M. Nedelyaev, Nadejda M. Pleskach, Irina M. Kuznetsova, Vladimir N. Uversky, Konstantin K. Turoverov, Mirya L. Kuranova

Molecular Medicine Faculty Publications

In this work, we performed a comparative study of the formation of PML bodies by full-length PML isoforms and their C-terminal domains in the presence and absence of endogenous PML. Based on the analysis of the distribution of intrinsic disorder predisposition in the amino acid sequences of PML isoforms, regions starting from the amino acid residue 395 (i.e., sequences encoded by exons 4–6) were assigned as the C-terminal domains of these proteins. We demonstrate that each of the full-sized nuclear isoforms of PML is capable of forming nuclear liquid-droplet compartments in the absence of other PML isoforms. These droplets possess …

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Nanoparticles For Coronavirus Control, Maryam Kianpour, Mohsen Akbarian, Vladimir N. Uversky Jan 2022

Nanoparticles For Coronavirus Control, Maryam Kianpour, Mohsen Akbarian, Vladimir N. Uversky

Molecular Medicine Faculty Publications

More than 2 years have passed since the SARS-CoV-2 outbreak began, and many challenges that existed at the beginning of this pandemic have been solved. Some countries have been able to overcome this global challenge by relying on vaccines against the virus, and vaccination has begun in many countries. Many of the proposed vaccines have nanoparticles as carriers, and there are different nano-based diagnostic approaches for rapid detection of the virus. In this review article, we briefly examine the biology of SARS-CoV-2, including the structure of the virus and what makes it pathogenic, as well as describe biotechnological methods of …

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Looking At The Pathogenesis Of The Rabies Lyssavirus Strain Pasteur Vaccins Through A Prism Of The Disorder-Based Bioinformatics, Surya Dhulipala, Vladimir N. Uversky Jan 2022

Looking At The Pathogenesis Of The Rabies Lyssavirus Strain Pasteur Vaccins Through A Prism Of The Disorder-Based Bioinformatics, Surya Dhulipala, Vladimir N. Uversky

Molecular Medicine Faculty Publications

Rabies is a neurological disease that causes between 40,000 and 70,000 deaths every year. Once a rabies patient has become symptomatic, there is no effective treatment for the illness, and in unvaccinated individuals, the case-fatality rate of rabies is close to 100%. French scientists Louis Pasteur and Émile Roux developed the first vaccine for rabies in 1885. If administered before the virus reaches the brain, the modern rabies vaccine imparts long-lasting immunity to the virus and saves more than 250,000 people every year. However, the rabies virus can suppress the host’s immune response once it has entered the cells of …

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Sars-Cov-2: A Master Of Immune Evasion, Alberto Rubio-Casillas, Elrashdy M. Redwan, Vladimir N. Uversky Jan 2022

Sars-Cov-2: A Master Of Immune Evasion, Alberto Rubio-Casillas, Elrashdy M. Redwan, Vladimir N. Uversky

Molecular Medicine Faculty Publications

Viruses and their hosts have coevolved for a long time. This coevolution places both the pathogen and the human immune system under selective pressure; on the one hand, the immune system has evolved to combat viruses and virally infected cells, while viruses have developed sophisticated mechanisms to escape recognition and destruction by the immune system. SARS-CoV-2, the pathogen that is causing the current COVID-19 pandemic, has shown a remarkable ability to escape antibody neutralization, putting vaccine efficacy at risk. One of the virus’s immune evasion strategies is mitochondrial sabotage: by causing reactive oxygen species (ROS) production, mitochondrial physiology is impaired, …

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Calcium-Bound S100p Protein Is A Promiscuous Binding Partner Of The Four-Helical Cytokines, Alexey S. Kazakov, Eugenia I. Deryusheva, Maria E. Permyakova, Andrey S. Sokolov, Victoria A. Rastrygina, Vladimir N. Uversky, Eugene A. Permyakov, Sergei E. Permyakov Jan 2022

Calcium-Bound S100p Protein Is A Promiscuous Binding Partner Of The Four-Helical Cytokines, Alexey S. Kazakov, Eugenia I. Deryusheva, Maria E. Permyakova, Andrey S. Sokolov, Victoria A. Rastrygina, Vladimir N. Uversky, Eugene A. Permyakov, Sergei E. Permyakov

Molecular Medicine Faculty Publications

S100 proteins are multifunctional calcium-binding proteins of vertebrates that act intracellularly, extracellularly, or both, and are engaged in the progression of many socially significant diseases. Their extracellular action is typically mediated by the recognition of specific receptor proteins. Recent studies indicate the ability of some S100 proteins to affect cytokine signaling through direct interaction with cytokines. S100P was shown to be the S100 protein most actively involved in interactions with some four-helical cytokines. To assess the selectivity of the S100P protein binding to four-helical cytokines, we have probed the interaction of Ca2+-bound recombinant human S100P with a panel of 32 …

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