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Assessment Of Dietary Patterns, Physical Activity And Obesity From A National Survey: Rural-Urban Health Disparities In Older Adults, Steven A. Cohen, Mary L. Greaney, Natalie J. Sabik

Public Health Faculty Publications

Background

Obesity is a critical public health issue, affecting over one-third of all Americans, and is an underlying cause of numerous health issues across the lifespan. For older adults, obesity is linked to premature declines in physical and mental health and cognitive functioning. The occurrence of obesity and related health behaviors and chronic diseases are higher in rural areas than in urban areas. Furthermore, rural areas of the United States have a higher proportion of older adults than urban areas. Few studies, to date, have explored rural-urban differences in the relationships between dietary patterns and obesity among older adults. Therefore, …

Perceptions Of Commercial And Recreational Fishers On The Potential Ecological Impacts Of The Block Island Wind Farm (Us), Talya S. Ten Brink, Tracey Dalton

Marine Affairs Faculty Publications

Offshore wind is gaining momentum in the United States as a viable source for meeting domestic energy needs. Although offshore wind farms have been developed in Europe and Asia, the Block Island Wind Farm (BIWF) is the first offshore wind farm built in North America. To improve marine resource management, it is critical to understand the impacts of the wind farm on marine resource users in context. Little is known about the impacts of offshore wind farms on marine resource users in the United States. This study investigates recreational and commercial fishers' perceptions of the impacts of the BIWF on …

Influence Of Commercial Dna Extraction Kit Choice On Prokaryotic Community Metrics In Marine Sediment, Gustavo Ramirez, Dennis Graham, Steven D’Hondt

Graduate School of Oceanography Faculty Publications

Commercial DNA extraction kits are widely used for cultivation‐free surveys of marine sediment. However, the consequences of popular extraction‐kit choices for sequence‐based biological inferences about marine sedimentary communities have not previously been exhaustively assessed. To address this issue, we extracted DNA from multiple paired subsamples of marine sediment using two popular commercial extraction kits (MO BIO Laboratories PowerSoil® DNA isolation kit and MP Biomedicals FastDNATM Spin Kit for Soil). We report comparisons of (1) total DNA yield, (2) extract purity, (3) gene‐targeted quantification, and (4) post‐sequencing ecological inferences in near‐seafloor (< 1 meter below seafloor [mbsf]) and subsurface (> 1 mbsf) marine sediment. In near‐seafloor sediment, the MP …

Asymmetric Biotic Interactions And Abiotic Niche Differences Revealed By A Dynamic Joint Species Distribution Model, Nina K. Lany, Phoebe Zarnetske, Erin M. Schliep, Robert N. Schaeffer, Colin M. Orians, David A. Orwig, Evan L. Preisser

Biological Sciences Faculty Publications

A species’ distribution and abundance are determined by abiotic conditions and biotic interactions with other species in the community. Most species distribution models correlate the occurrence of a single species with environmental variables only, and leave out biotic interactions. To test the importance of biotic interactions on occurrence and abundance, we compared a multivariate spatiotemporal model of the joint abundance of two invasive insects that share a host plant, hemlock woolly adelgid (HWA; Adelges tsugae) and elongate hemlock scale (EHS; Fiorina externa), to independent models that do not account for dependence among co‐occurring species. The joint model revealed …

Benthic Habitat Mapping And Its Applications To Coastal Resource Management, Monique Lafrance Bartley

Open Access Dissertations

This dissertation is comprised of three chapters focused on benthic habitat mapping of coastal waters within northeast region of the United States to support science-based regulatory and management strategies. The first chapter is entitled: Shallow water benthic habitat mapping utilizing the Coastal and Marine Ecological Classification Standard (CMECS) to establish baseline conditions post-Hurricane Sandy at Fire Island National Seashore, New York. In response to Hurricane Sandy, a benthic habitat mapping study was conducted at Fire Island National Seashore (FIIS), New York, representing one of the first comprehensive mapping efforts undertaken by the National Park Service. FIIS was of particular interest …

Fine-Scale Habitat Comparison Of Two Sympatric Cottontail Species In Eastern Connecticut, Amy E. Gottfried Mayer, Thomas J. Mcgreevy Jr., Mary E. Sullivan, Bill Buffum, Thomas P. Husband

Natural Resources Science Faculty Publications

Changing landscapes in the Northeastern United States over the past century have had a profound effect on the abundance and distribution of native wildlife species that prefer early successional habitat, including New England cottontail (Sylvilagus transitionalis). Populations of New England cottontail have been in decline for several decades, whereas during this same time period the nonnative eastern cottontail (S. floridanus) range has expanded. We conducted intensive vegetation analyses at 17 known locations of New England cottontail and 19 known locations of eastern cottontail in Connecticut to better describe their chosen habitat and identify any difference in habitat used by the …

Individual And Non‐Additive Effects Of Exotic Sap‐Feeders On Root Functional And Mycorrhizal Traits Of A Shared Conifer Host, Robert N. Schaeffer, Claire M. Wilson, Laura Radville, Mauri Barrett, Elizabeth Whitney, Sofia Roitman, Esther R. Miller, Benjamin E. Wolfe, Carol S. Thornber, Colin M. Orians, Evan L. Preisser

Biological Sciences Faculty Publications

1. Forest pests drive tree mortality through disruption of functional traits linked to nutrient acquisition, growth and reproduction. The impacts of attack by individual or multiple above‐ground herbivores on root functional traits critical to tree health have received little attention. This is especially true for exotic herbivores, organisms often found in disturbed forests.
2. We excavated whole‐root systems from eastern hemlock (Tsuga canadensis) individuals experimentally infested with hemlock woolly adelgid (HWA: Adelges tsugae) and elongate hemlock scale (EHS: Fiorina externa) individually, or in combination, for periods of 2 and 4 years. Below‐ground root biomass, functional traits and storage …

Advances In The Application Of Amino Acid Nitrogen Isotopic Analysis In Ecological And Biogeochemical Studies, Naohiko Ohkouchi, Yoshito Chikaraishi, Hilary Close, Brian Fry, Thomas Larsen, Daniel J. Madigan, Matthew D. Mccarthy, Kelton Mcmahon, Toshi Nagata, Yuichi I. Naito, Nanako O. Ogawa, Brian N. Popp, Shawn Steffan, Yoshinori Takano, Ichiro Tayasu, Alex S. J. Wyatt, Yasuhiko T. Yamaguchi, Yusuke Yokoyama

Graduate School of Oceanography Faculty Publications

Compound-specific isotopic analysis of amino acids (CSIA-AA) has emerged in the last decade as a powerful approach for tracing the origins and fate of nitrogen in ecological and biogeochemical studies. This approach is based on the empirical observation that source amino acids (SAAs) (i.e., phenylalanine), fractionate ¹⁵N very little (< 0.5‰) during trophic transfer, whereas trophic AAs (TAAs) (i.e., glutamic acid), are greatly (∼6–8‰) enriched in ¹⁵N during each trophic step. The differential fractionation of these two AA groups can provide a valuable estimate of consumer trophic position that is internally indexed to the baseline δ¹⁵N value of the integrated food web. In this paper, we critically review the analytical methods for …

Determinants Of Spread In An Urban Landscape By An Introduced Lizard, Jason J. Kolbe, Paul Vanmiddlesworth, Andrew C. Battles, James T. Stroud, Bill Buffum, Richard T. T. Forman, Jonathan B. Losos

Biological Sciences Faculty Publications

Context: Urban landscapes are a mixture of built structures, human-altered vegetation, and remnant semi-natural areas. The spatial arrangement of abiotic and biotic conditions resulting from urbanization doubtless influences the establishment and spread of non-native species in a city.

Objectives: We investigated the effects of habitat structure, thermal microclimates, and species coexistence on the spread of a non-native lizard (Anolis cristatellus) in the Miami metropolitan area of South Florida (USA).

Methods: We used transect surveys to estimate lizard occurrence and abundance on trees and to measure vegetation characteristics, and we assessed forest cover and impervious surface using GIS. We …

Nitrogen And Warming Effects On Nitrous Oxide Production Associated With Coastal Macroinvertibrates, Melanie H. Gárate

Open Access Master's Theses

Bivalve shellfish potentially reduce excess nitrogen in the water column, however they can also be involved in the emission of nitrous oxide (N₂O), a potent greenhouse gas. Environmental controls on N₂O production from bivalves have not been well quantified. We tested responses of N2O production by three bivalves (Mytilus edulis, Mercenaria mercenaria and Crassostrea virginica) to nitrogen (N) loading and/or warming after immediate (1 day) and short-term (14-28 days) exposure. This two-factor laboratory study had four treatments: (1) ammonium nitrate (N) addition (targeting 100μM-N), (2) warming (22^oC), (3) N addition + …

Temperature Effects On The Proteome Of Ciona Intestinalis, Chelsea Lopez

Open Access Master's Theses

Ciona intestinalis is a solitary ascidian that lives in temperate waters around the globe. C. intestinalis in Rhode Island exhibit normal embryonic development at water temperatures between 10° C and 18° C. This thesis is interested in what changes occur in the ovary tissue at temperatures above 18° C and below 10° C. A preliminary study to assess the reproductive fitness of C. intestinalis, found that C. intestinalis raised at 22 °C had lower embryo viability when compared to the normal temperature range. 22 °C was chosen to mimic the high temperature predicted in some climate change models (IPCC, …

Antimicrobial Resistance Patterns And Protective Effects Of Statins In Bacteremic Patients, Ajinkya Pawar

Open Access Dissertations

The significant increase in antimicrobial resistance over the past few years is a serious global public health concern, particularly as the development of new antimicrobial agents had been slow for many years. Infections with resistant organisms are associated with poor clinical outcomes and higher cost burdens. Determining antimicrobial resistance patterns can help identify problem areas and modify treatment practices to improve clinical outcomes. Additionally, identifying adjunctive therapies can also help improve clinical outcomes among infected patients. The objectives, hypotheses, methods and results of this dissertation are threefold:

Manuscript 1: The objective was to analyze antimicrobial resistance trends in E. faecalis …

Diet Of The Endangered Big-Headed Turtle Platysternon Megacephalum, Yik-Hei Sung, Billy C.H. Hau, Nancy E. Karraker

Natural Resources Science Faculty Publications

Populations of the big-headed turtle Platysternon megacephalum are declining at unprecedented rates across most of its distribution in Southeast Asia owing to unsustainable harvest for pet, food, and Chinese medicine markets. Research on Asian freshwater turtles becomes more challenging as populations decline and basic ecological information is needed to inform conservation efforts. We examined fecal samples collected from P. megacephalum in five streams in Hong Kong to quantify the diet, and we compared the germination success of ingested and uningested seeds. Fruits, primarily of Machilus spp., were most frequently consumed, followed by insects, plant matter, crabs and mollusks. The niche …

Metagenomic Signatures Of Bacterial Adaptation To Life In The Phyllosphere Of A Salt-Secreting Desert Tree, Omri M. Finkel, Tom O. Delmont, Anton F. Post, Shimshon Belkin

Graduate School of Oceanography Faculty Publications

The leaves of Tamarix aphylla, a globally distributed, salt-secreting desert tree, are dotted with alkaline droplets of high salinity. To successfully inhabit these organic carbon-rich droplets, bacteria need to be adapted to multiple stress factors, including high salinity, high alkalinity, high UV radiation, and periodic desiccation. To identify genes that are important for survival in this harsh habitat, microbial community DNA was extracted from the leaf surfaces of 10 Tamarix aphylla trees along a 350-km longitudinal gradient. Shotgun metagenomic sequencing, contig assembly, and binning yielded 17 genome bins, six of which were >80% complete. These genomic bins, representing three phyla …

Different Populations Of Blacklegged Tick Nymphs Exhibit Differences In Questing Behavior That Have Implications For Human Lyme Disease Risk, Isis M. Arsnoe, Graham J. Hickling, Howard S. Ginsberg, Richard Mcelreath, Jean I. Tsao

Plant Sciences and Entomology Faculty Publications

Animal behavior can have profound effects on pathogen transmission and disease incidence. We studied the questing (= host-seeking) behavior of blacklegged tick (Ixodes scapularis) nymphs, which are the primary vectors of Lyme disease in the eastern United States. Lyme disease is common in northern but not in southern regions, and prior ecological studies have found that standard methods used to collect host-seeking nymphs in northern regions are unsuccessful in the south. This led us to hypothesize that there are behavior differences between northern and southern nymphs that alter how readily they are collected, and how likely they are …

Landscape Pattern And Change Through Integration Of Remote Sensing And Stone Wall Feature Identification, Rebecca J. Trueman

Open Access Master's Theses

Stone walls are relics of an agricultural civilization that once flourished in New England. By identifying the locations of both historical and present day stone walls, compositions of post-agricultural landscapes common across the New England region can be assessed with inclusion of historic human-land use interactions. I selected the town of New Shoreham, known as Block Island, as the study site for this thesis. Block Island is located approximately 14.5 km south of the Rhode Island mainland. The Island has rich land use history which resulted in an extensive network of stone walls still present across the landscape. Through visual …

The Influence Of Salt Marsh Fucoid Algae (Ecads) On Sediment Dynamics Of Northwest Atlantic Marshes, M. C. Tyrrell, Carol S. Thornber, J. A. Burkhardt, M. Congretel

Biological Sciences Faculty Publications

Resilience is currently a key theme within salt marsh ecological studies. Understanding the factors that affect salt marsh accretion and elevation gains are of paramount importance if management of these ecosystems is to be successful under increasing synergistic stresses of storm surge, inundation period, and eutrophication. We present the results of salt marsh fucoid algae (ecads) removal experiments on Spartina alterniflora abundance, production and decomposition and the sedimentary dynamics of two marshes on Cape Cod, Massachusetts. The presence of the thick layer of marsh fucoids had a significant and positive influence on sediment deposition, accretion, concentration of water column particulates, …

The Marine Microbial Eukaryote Transcriptome Sequencing Project (Mmetsp): Illuminating The Functional Diversity Of Eukaryotic Life In The Oceans Through Transcriptome Sequencing, Patrick J. Keeling, Bethany D. Jenkins, Susanne Menden-Deuer, Et Al

Cell and Molecular Biology Faculty Publications

Funding: This project was funded by the Gordon and Betty Moore Foundation (GBMF; Grants GBMF2637 and GBMF3111) to the National Center for Genome Resources (NCGR) and the National Center for Marine Algae and Microbiota (NCMA). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Authors apart from NCGR and NCMA affiliates, FB and HMW (who performed 18S rRNA gene analyses), are community members who submitted samples for sequencing, including members of the advisory committee, but did not receive GBMF funds directly in support of these efforts.

Profiling Gene Expression To Distinguish The Likely Active Diazotrophs From A Sea Of Genetic Potential In Marine Sediments, S M. Brown, Bethany D. Jenkins

Cell and Molecular Biology Faculty Publications

Nitrogen (N) cycling microbial communities in marine sediments are extremely diverse, and it is unknown whether this diversity reflects extensive functional redundancy. Sedimentary denitrifiers remove significant amounts of N from the coastal ocean and diazotrophs are typically regarded as inconsequential. Recently, N fixation has been shown to be a potentially important source of N in estuarine and continental shelf sediments. Analysis of expressed genes for nitrite reductase (nirS) and a nitrogenase subunit (nifH) was used to identify the likely active denitrifiers and nitrogen fixers in surface sediments from different seasons in Narragansett Bay (Rhode Island, USA). …

Using Non-Dietary Gastropods In Coastal Shell Middens To Infer Kelp And Seagrass Harvesting And Paleoenvironmental Conditions, Amira F. Ainis, René Vellanoweth, Queeny G. Lapeña, Carol S. Thornber

Biological Sciences Faculty Publications

Archaeologists analyzing shell middens typically focus on larger (>2 cm) mollusks to examine subsistence practices, impacts on littoral habitats, and paleoenvironmental conditions as well as a host of other natural and cultural phenomena. Small (<2 cm), non-dietary gastropods in archaeological shell middens also provide important clues regarding human resource procurement in littoral areas and coastal paleoenvironments. We present data from two sites on the California Channel Islands to demonstrate the range of information that can be gained by analyzing small gastropod shells. Identifications revealed the remains of over 4500 non-dietary small gastropods from 75 taxa. Human harvesting of marine macrophytes is suggested by the presence of 18 species that are predominantly associated with seaweeds and seagrasses. Quantification measures revealed high diversity and equitability indices, oscillating taxonomic richness, and decreasing densities through time at both sites. Likelihood ratio tests revealed differences in assemblage composition between Early Holocene and later components at one site, and demonstrated similarities in the relative composition of non-dietary shell assemblages between both sites during the Middle Holocene. Incorporating detailed studies of less conspicuous “incidental” shellfish remains in archaeological midden studies has the potential to contribute to our understanding of past human land use practices and littoral paleoecology. Our findings are applicable to archaeologists working in coastal settings around the world, as well as marine ecologists interested in intertidal paleo-habitats and kelp forest ecosystems.

Untangling Intra- And Interspecific Effects On Body Size Clines Reveals Divergent Processes Structuring Convergent Patterns In Anolis Lizards, Martha M. Muñoz, Johanna E. Wegener, Adam C. Algar

Biological Sciences Faculty Publications

Bergmann’s rule—the tendency for body size to increase in colder environments—remains controversial today, despite 150 years of research. Considerable debate has revolved around whether the rule applies within or among species. However, this debate has generally not considered that clade-level relationships are caused by both intra- and interspecific effects. In this article, we implement a novel approach that allows for the separation of intra- and interspecific components of trait-environment relationships. We apply this approach to body size clines in two Caribbean clades of Anolis lizards and discover that their similar body size gradients are constructed in very different ways. We …

Impacts Of Management On Fisheries Diversity As A Factor Affecting Social Resilience In Communities Of The Southern New England Region, Tarsila Seara

Open Access Dissertations

The present research aims at examining potential impacts of fisheries management on the diversity of species exploited by fishermen in Southern New England, and examining the relationship between this diversity and the resilience of fishermen and, consequently, communities in adapting to changes in their social and physical environments. One way fishermen are able to adapt to environmental and socio-economic changes is through diversification of the catch. The current study argues that certain management plans, especially those focused on limited entry strategies, have been reducing fishermen’s adaptive flexibility, therefore reducing fishermen’s resilience and that of their communities. Two hypotheses were developed: …

A Case Study Of Student And Teacher Relationships And The Effect On Student Learning, Patricia Brady Gablinske

Open Access Dissertations

This research study explored the affective domain of teacher-student relationships using a single case study design. This single case study produced a synthesis of information that guides a classroom teacher in the development and maintenance of her relationships with her students. The resulting analysis and interpretation provided a description of major themes that developed regarding strong teacher student relationships, as well as, specific components to the interactions considered essential for the student’s learning environment.

The outcome of this study is an account of experiences and procedures that guide the development and maintenance of relationships between a teacher and her students. …

Associations Between Family Food Behaviors, Maternal Depression, And Child Weight Among Low-Income Children, Karen Mccurdy, Kathleen S. Gorman, Tiffani S. Kisler, Elizabeth Metallinos-Katsaras

Human Development and Family Science Faculty Publications

Although low-income children are at greater risk for overweight and obesity than their higher income counterparts, the majority of poor children are not overweight. The current study examined why such variation exists among diverse young children in poor families. Cross-sectional data were collected on 164 low-income, preschool aged children and their mothers living in two Rhode Island cities. Over half of the sample was Hispanic (55%). Mothers completed measures of family food behaviors and depression while trained assistants collected anthropometric data from children at seven day care centers and a Supplemental Nutrition Assistance Program outreach project. Multivariate analysis of covariance …

Sensory Basis For Detection Of Benthic Prey In Two Lake Malawi Cichlids., Margot A. B. Schwalbe, Jacqueline F. Webb

Biological Sciences Faculty Publications

The adaptive radiations of African cichlids resulted in a diversity of feeding morphologies and strategies, but the role of sensory biology in prey detection and feeding ecology remains largely unexplored. Two endemic Lake Malawi cichlid genera, Tramitichromis and Aulonocara, feed on benthic invertebrates, but differ in lateral line morphology (narrow and widened lateral line canals, respectively) and foraging strategy. The hypothesis that they use their lateral line systems differently was tested by looking at the relative contribution of the lateral line system and vision in prey detection by Tramitichromis sp. and comparing results to those from a complementary study using …

Hemlock Woolly Adelgid And Elongate Hemlock Scale Induce Changes In Foliar And Twig Volatiles Of Eastern Hemlock, Joshua Pezet, Joseph Elkinton, Sara Gomez, E. Alexa Mckenzie, Michael Lavine, Evan Preisser

Biological Sciences Faculty Publications

Eastern hemlock (Tsuga canadensis) is in rapid decline because of infestation by the invasive hemlock woolly adelgid (Adelges tsugae; 'HWA') and, to a lesser extent, the invasive elongate hemlock scale (Fiorinia externa; 'EHS'). For many conifers, induced oleoresin-based defenses play a central role in their response to herbivorous insects; however, it is unknown whether eastern hemlock mobilizes these inducible defenses. We conducted a study to determine if feeding by HWA or EHS induced changes in the volatile resin compounds of eastern hemlock. Young trees were experimentally infested for 3 years with HWA, EHS, or neither insect. Twig and needle resin …

Examination Of Ulva Bloom Species Richness And Relative Abundance Reveals Two Cryptically Co- Occurring Bloom Species In Narragansett Bay, Rhode Island, Michele Guidone, Carol S. Thornber

Biological Sciences Faculty Publications

Blooms caused by the green macroalga Ulva pose a serious threat to coastal ecosystem 20 around the world. Despite numerous studies of the causes and consequences of these blooms, we still have a limited understanding of Ulva bloom species richness and abundance due to difficulties in identifying Ulva species using morphological features. Along the northeastern U.S. coastline, all blooms of distromatic Ulva blades were previously identified as U. lactuca. Recent molecular sequencing, however, discovered the presence of additional distromatic Ulva species. Therefore, in order to determine the relative abundance of Ulva species within blooms, we conducted monthly surveys at four …

Hitting The Right Target: Taxonomic Challenges For, And Of, Plant Invasions, Peter Pyšek, Philip E. Hulme, Laura A. Meyerson, Gideon F. Smith, James S. Boatwright, Neil R. Crouch, Estrela Figueiredo, Llewellyn C. Foxcroft, Vojtěch Jarošik, David M. Richardson, Jan Suda, John R. U. Wilson

Natural Resources Science Faculty Publications

This paper explores how a lack of taxonomic expertise, and by implication a dearth of taxonomic products such as identification tools, has hindered progress in understanding and managing biological invasions. It also explores how the taxonomic endeavour could benefit from studies of invasive species. We review the literature on the current situation in taxonomy with a focus on the challenges of identifying alien plant species and explore how this has affected the study of biological invasions. Biosecurity strategies, legislation dealing with invasive species, quarantine, weed surveillance and monitoring all depend on accurate and rapid identification of non-native taxa. However, such …

Middle School Food Environments And Racial/Ethnic Differences In Sugar-Sweetened Beverage Consumption: Findings From The Healthy Choices Study, Tracy K. Richmond, Jennifer L. Spadano-Gasbarro, Courtney E. Walls, S. Bryn Austin, Mary L. Greaney, Monica L. Wang, Solomon Mezegebu, Karen E. Peterson

Kinesiology Faculty Publications

Background: Prior studies have demonstrated disproportionate clustering of fast food outlets around schools.

Purpose: The purpose of this study is to determine if racial/ethnic differences in middle school student self-reported sugar-sweetened beverage (SSB) consumption is explained by differential distributions of food outlets surrounding their schools.

Methods: Baseline (2005) data were analyzed from 18,281 middle school students in 47 Massachusetts schools participating in Healthy Choices, an obesity prevention program. Linear mixed effects models were used to examine the association of individual race/ethnicity and daily SSB consumption and the potential mediating effect of the density of food outlets (the number of fast …

Using Molecular Tools To Elucidate Controls On Microbes Driving The Nitrogen Cycle In Marine Sediments, Shelley Marie Brown

Open Access Dissertations

Marine sediments harbor metabolically versatile bacteria whose activities can influence the cycle of nutrients on global scales. Microbial communities driving nitrogen (N) cycling are extremely diverse, thus making it difficult to identify the functional groups and elucidate controls on their activity. Denitrifiers in sediments remove significant amounts of N from the coastal ocean, while diazotrophs are typically considered inconsequential. Recently, N fixation has been shown to be a potentially important source of N in coastal sediments, however, the environmental drivers controlling this process are poorly understood. The goal of this dissertation was to identify and target the likely active denitrifiers …