Digital Commons Network^™

Efficacy And Safety Of Low-Dose Versus High-Dose Hydrocortisone To Treat Hypotension In Neonates: A Protocol For A Systematic Review And Meta-Analysis, Katelyn Sushko, Nada Al-Rawahi, Kristi L. Watterberg, John Van Den Anker, Catherine Litalien, Jacques Lacroix, Abdul Razak, Samira Samiee-Zafarghandy

Pediatrics Research and Scholarship

BACKGROUND: Impaired adrenal function is a well-described entity in critically ill term and preterm neonates with systemic hypotension. The standard treatment for neonatal hypotension includes volume expanders and vasopressors. Recent evidence supports the use of glucocorticoids for the primary or rescue treatment of neonatal hypotension associated with impaired adrenal function. However, inconsistency regarding the prescribed dosing regimen to provide the best balance between efficacy and safety in this vulnerable population remains an area of concern.

METHODS: We will conduct a systematic review and meta-analysis to evaluate low-dosing compared with high-dosing regimens of hydrocortisone for the treatment of hypotension in critically …

Nuanced Networks: How Social Relationships And Power Influence Participation In Private Lands Conservation Programs, Ellen Loechner

Geography ETDs

Because the majority of land in the Midwestern United States is privately owned, the responsibility for creating and maintaining space for wildlife conservation falls upon private landowners. Numerous state and federal agencies and non-governmental organizations (NGOs) host private lands conservation programs that offer landowners financial and technical support to complete conservation projects on their property (Echols, Front, and Cummins 2019). While the individual motivations and priorities of a landowner may inform how they use and manage their property, a landowner’s ability to connect with the agricultural and conservation communities may determine if they have the opportunity to participate in these …

Microrna Analysis Of Human Stroke Brain Tissue Resected During Decompressive Craniectomy/Stroke-Ectomy Surgery, Andrew P. Carlson, William Mckay, Jeremy S. Edwards, Radha Swaminathan, Karen S. Santacruz, Ron L. Mims, Howard Yonas, Tamara Roitbak

Pathology Research and Scholarship

BACKGROUND: Signaling pathways mediated by microRNAs (miRNAs) have been identified as one of the mechanisms that regulate stroke progression and recovery. Recent investigations using stroke patient blood and cerebrospinal fluid (CSF) demonstrated disease-specific alterations in miRNA expression. In this study, for the first time, we investigated miRNA expression signatures in freshly removed human stroke brain tissue.

METHODS: Human brain samples were obtained during craniectomy and brain tissue resection in severe stroke patients with life-threatening brain swelling. The tissue samples were subjected to histopathological and immunofluorescence microscopy evaluation, next generation miRNA sequencing (NGS), and bioinformatic analysis.

RESULTS: miRNA NGS analysis detected …

Bioengineered Models To Study Microenvironmental Regulation Of Glioblastoma Metabolism, Joseph Chen, Hyunchul Lee, Philipp Schmitt, Caleb J. Choy, Donald M. Miller, Brian J. Williams, Elaine L. Bearer, Hermann B. Frieboes

Pathology Research and Scholarship

Despite extensive research and aggressive therapies, glioblastoma (GBM) remains a central nervous system malignancy with poor prognosis. The varied histopathology of GBM suggests a landscape of differing microenvironments and clonal expansions, which may influence metabolism, driving tumor progression. Indeed, GBM metabolic plasticity in response to differing nutrient supply within these microenvironments has emerged as a key driver of aggressiveness. Additionally, emergent biophysical and biochemical interactions in the tumor microenvironment (TME) are offering new perspectives on GBM metabolism. Perivascular and hypoxic niches exert crucial roles in tumor maintenance and progression, facilitating metabolic relationships between stromal and tumor cells. Alterations in extracellular …

Relief And Reward Drinking Across Time In Community And Treatment-Seeking Samples, Julia E. Swan

Psychology ETDs

Classifying people with alcohol use disorder into homogenous groups based on observed characteristics (i.e., phenotypes) could match individuals to specific treatments. The reward-relief phenotype classifies individuals based on whether they drink to enhance rewarding experiences and/or to relieve negative states. The current study is a secondary data analysis of a community sample of non-treatment seeking heavy drinkers (n = 189) and two randomized clinical trial samples of individuals with alcohol use disorder (n = 1726, 1383) that aimed to determine if the reward-relief phenotype is identified across samples, is stable over time, and predicts long-term alcohol consumption and consequences. We …

Comparative Phylogeography Across Multiple Scales: Small Mammals, Their Ecology, Pathogens, And Drivers Of Diversification, Schuyler W. Liphardt

Biology ETDs

Comparative phylogeography has historically been defined as the study of how genetic variation of co-distributed species has been shaped by biogeographical history. This is a mature field of study out of which several techniques have been developed directed at identifying the role ecology and geography in diversification patterns across time. I employ the tools developed in classical comparative phylogeography across multiple taxonomic scales and across regions that historically have been understudied. My first two chapters study the complex history of host-switching, codiversification, and reassortment of hantaviruses in their mammal hosts across North America. By taking a host-centric comparative phylogeographic approach …

Quantification Of Soil Microbial Biomass In The Mcmurdo Dry Valleys, Antarctica, Emily Ruth Reynebeau

Biology ETDs

Quantifying microbial biomass is important for understanding microbial contribution to nutrient cycling and population flux. Widely accepted biomass estimation methods are sufficient for well-studied and high biomass systems, though are often not sensitive enough for systems with extremely low biomass. The McMurdo Dry Valleys of Antarctica are characterized as an extremely harsh environment with severe restrictions on life, with perhaps some of the lowest biomass in the world. We measured prokaryote biomass across Taylor and Wright Valleys using four estimation metrics to determine the most appropriate method for this ecosystem. The chloroform fumigation method was not sensitive enough for the …

Breathing Can Be Dangerous: Coccidioides And The Diverse Community Of The Lung Mycobiome, Paris S. Salazar-Hamm

Biology ETDs

Coccidioidomycosis, caused by soil-dwelling species of Coccidioides, is endemic to the Southwestern United States. It can present as a mild respiratory illness to life-threatening disseminated infections.Multi-gene phylogenetics and tracing patient histories revealed the presence of both Coccidioides species in New Mexico, and Native Americans may represent an unrecognized risk group. A PCR method developed distinguishes Coccidioides species by targeting regions of the mitochondrial genome. Recent advancements in molecular genetics solidifies lungs are not sterile. Illumina community data from 199 small mammals from Arizona, California, and New Mexico demonstrated a great diversity of fungi inhabiting lung tissues. One dominant order, Onygenales, …

Effectiveness Of Growth Mindset Intervention On Elementary Students’ Academic Performance: A Systematic Review, Salma Yeasmin

Teacher Education, Educational Leadership & Policy ETDs

The lack of academic success of the U.S. adolescents and wide achievement gap have become a persistent problem of the United States. Therefore, several educational reforms have been made to increase U.S. students’ academic performance as well as close achievement gap. However, despite the various reforms and efforts that have been enacted in educational sectors, U.S. students’ academic performance seemed not enough. Therefore, psychological factors were considered in the educational reform strategy, in which promoting growth mindset among students has become a way to accelerate students’ academic performance as well as reduce the achievement gap. Since then, numerous growth mindset …

Tracing Transmission Of Sin Nombre Virus And Discovery Of Infection In Multiple Rodent Species, Samuel M Goodfellow, Robert A. Nofchissey, Kurt C. Schwalm, Joseph A. Cook, Jonathan L. Dunnum, Yan Guo, Chunyan Ye, Gregory J. Mertz, Kartik Chandran, Michelle Harkins, Daryl B. Domman, Darrell L. Dinwiddie, Steven B. Bradfute

Pediatrics Research and Scholarship

Sin Nombre orthohantavirus (SNV), a negative-sense, single-stranded RNA virus that is carried and transmitted by the North American deer mouse Peromyscus maniculatus, can cause infection in humans through inhalation of aerosolized excreta from infected rodents. This infection can lead to hantavirus cardiopulmonary syndrome (HCPS), which has an ∼36% case-fatality rate. We used reverse transcriptase quantitative PCR (RT-qPCR) to confirm SNV infection in a patient and identified SNV in lung tissues in wild-caught rodents from potential sites of exposure. Using viral whole-genome sequencing (WGS), we identified the likely site of transmission and discovered SNV in multiple rodent species not previously known …

Left-Handed Dna: The Right Way Forward, Tracy Mallette

Shared Knowledge Conference

Rapid detection of emerging pathogens, environmental monitoring of waterways, and cancer treatments that operate on a cell by cell basis are all technological advances which could be achieved with DNA-based molecular computers. DNA encodes the building blocks of life and researchers have already shown that it can be used as a powerful tool for us to build nanoscale devices that can interact with the molecular world. However, DNA-based molecular devices are susceptible to degradation by natural defense mechanisms called nucleases. This makes operating in the desired biological settings challenging and has thus limited their practical application. My NSF-funded research in …

Moderate Prenatal Alcohol Exposure Alters Protein Expression Of Stress And Immune Factors In The Amygdala Following Early Life Stress., Annette Fernandez

Shared Knowledge Conference

Fetal alcohol spectrum disorder (FASD) is the outcome of prenatal alcohol exposure (PAE) that results in cognitive, mood and neuroimmune disorders. PAE negatively impacts the brain stress-response system by dysregulating the expression of stress neuropeptides such as corticotropin releasing hormone (CRH), and neuroimmune factors including interlukin-1beta (IL-1β). Both CRH and IL-1β are suspected to play a role in chronic anxiety and depression. In animal models, studies have characterized the key brain region, the amygdala, in regulating stress and emotion such as anxiety and depression. Prior research demonstrates that stress enhances amygdala activity and alters the expression of neuroimmune factors. However, …

Evaluation Of Thermal Infrared Imaging From Uninhabited Aerial Vehicles For Arboreal Wildlife Surveillance, Blair Mirka

Shared Knowledge Conference

An important component of wildlife management and conservation is monitoring the health and population size of wildlife species. Monitoring the population size of an animal group can inform researchers of habitat use, potential changes in habitat and the resulting behavioral adaptation, individual health, and the effectiveness of conservation efforts. Arboreal primates are difficult to monitor as their habitat is often poorly accessible and most primate species have some degree of camouflage, making them hard to observe in and below the tree canopy. Surveys conducted using uninhabited aerial vehicles (UAVs) equipped with thermal infrared (TIR) cameras can help overcome these limitations …

Distributional Effect Of Exposure To Cafos: A Case Study Of Large Dairies In New Mexico, Suraj Ghimire

Shared Knowledge Conference

The industrialization of the agricultural sector has turned into a double-edged sword for the future of humanity on this planet. On the one hand, without industrialized agriculture, there would be insufficient food and fiber to feed the growing human population. On the other hand, the rapid expansion of large-scale farms has resulted in an unprecedented amount of negative externalities impacting the environment and human health. While the benefits of abundance have been enjoyed evenly across the board, the negative impacts are borne mainly by and concentrated in the historically underprivileged communities. The goal of this research is to look at …

Integrin Activation Is An Essential Component Of Sars-Cov-2 Infection, Peter Simons, Derek A. Rinaldi, Virginie Bondu, Alison M. Kell, Steven Bradfute, Diane S. Lidke, Tione Buranda

Pathology Research and Scholarship

SARS-CoV-2 infection depends on binding its spike (S) protein to angiotensin-converting enzyme 2 (ACE2). The S protein expresses an RGD motif, suggesting that integrins may be co-receptors. Here, we UV-inactivated SARS-CoV-2 and fluorescently labeled the envelope membrane with octadecyl rhodamine B (R18) to explore the role of integrin activation in mediating cell entry and productive infection. We used flow cytometry and confocal microscopy to show that SARS-CoV-2^R18 particles engage basal-state integrins. Furthermore, we demonstrate that Mn2+, which induces integrin extension, enhances cell entry of SARS-CoV-2^R18. We also show that one class of integrin antagonist, which binds to …

Data Sharing Workshop, Lori D. Sloane

Posters and Presentations

Learning Objectives:

• Identify the key elements of planning clinical research data management
• Outline steps needed to develop a data collection plan
• Locate standards, validated instruments, and coding schemes for research
• Describe and implement the different components of a data management plan
• Introduction to OpenRefine data tool

Tetraspanin Cd82 Regulates S1pr, Chelsea A. Saito-Reis, Victoria D. Balise, Erica M. Pascetti, Magdalena Jiminez, Jennifer M. Gillette

Pathology Research and Scholarship

Hematopoietic stem and progenitor cell (HSPC) mobilization into the blood occurs under normal physiological conditions and is stimulated in the clinic to enable the isolation of HSPCs for transplantation therapies. In the present study, we identify the tetraspanin CD82 as a novel regulator of HSPC mobilization. Using a global CD82 knockout (CD82KO) mouse, we measure enhanced HSPC mobilization after granulocyte-colony stimulating factor (G-CSF) or AMD3100 treatment, which we find is promoted by increased surface expression of the sphingosine 1-phosphate receptor 1 (S1PR

Tet2 And Dnmt3a Mutations Exert Divergent Effects On Dna Repair And Sensitivity Of Leukemia Cells To Parp Inhibitors, Silvia Maifrede, Bac Viet Le, Margaret Nieborowska-Skorska, Konstantin Golovine, Katherine Sullivan-Reed, Wangisa M B Dunuwille, Joseph Nacson, Michael Hulse, Kelsey Keith, Jozef Madzo, Lisa Beatrice Caruso, Zachary Gazze, Zhaorui Lian, Antonella Padella, Kumaraswamy N. Chitrala, Boris A. Bartholdy, Ksenia Matlawska-Wasowska, Daniela Di Marcantonio, Giorgia Simonetti, Georg Greiner, Stephen M Sykes, Peter Valent, Elisabeth M. Paietta, Martin S. Tallman, Hugo F. Fernandez, Mark R. Litzow, Mark D. Minden, Jian Huang, Giovanni Martinelli, George S. Vassiliou, Italo Tempera, Katarzyna Piwocka, Neil Johnson, Grant A. Challen, Tomasz Skorski

Pediatrics Research and Scholarship

Somatic variants in TET2 and DNMT3A are founding mutations in hematological malignancies that affect the epigenetic regulation of DNA methylation. Mutations in both genes often co-occur with activating mutations in genes encoding oncogenic tyrosine kinases such as FLT3ITD, BCR-ABL1, JAK2V617F , and MPLW515L , or with mutations affecting related signaling pathways such as NRASG12D and CALRdel52 . Here, we show that TET2 and DNMT3A mutations exert divergent roles in regulating DNA repair activities in leukemia cells expressing these oncogenes. Malignant TET2-deficient cells displayed downregulation of BRCA1 and LIG4, resulting in reduced activity of BRCA1/2-mediated homologous recombination (HR) and DNA-PK-mediated non-homologous …

Trade-Offs In Growth And Development: A Life History Approach, Anna Medendorp Rautman

Anthropology ETDs

Life history theory (LHT) is the conceptualization of tradeoffs in resources allocation. Plasticity, the ability of phenotypes to vary in response to environmental cues, is integral to LHT. Prior to completed maturation, resources are distributed towards maintenance or somatic maturation, a combination of growth and development (G&D). This dissertation presents an examination of intragroup variation in the G&D of the dental and skeletal systems within a sample of 20th century White females to consider whether they provide evidence for tradeoffs within the subadult interval. In a series of individual research chapters, I consider the following: 1) how does the relative …

Servitudes Done "Proper"Ly: Propriety, Not Contract Law, Elizabeth Elia

Faculty Scholarship

The law of servitudes has changed dramatically in the last hundred years. Legal scholars have been successful in documenting these changes in case law, but less successful in articulating a unified set of principles that guide the current law of servitudes. One explanation that received significant attention is the idea that efficient recording systems in the United States have liberated property owners from the constraints of traditional property laws disfavoring servitudes and have allowed these owners the broader freedoms of contract law. However, this explanation, and the sweeping common law reforms that flow from it, have not resonated with courts. …

Decreased Incidence Of Infections Caused By Pathogens Transmitted Commonly Through Food During The Covid-19 Pandemic - Foodborne Diseases Active Surveillance Network, 10 U.S. Sites, 2017-2020, Logan C. Ray, Jennifer P. Collins, Patricia M. Griffin, Hazel J. Shah, Michelle M. Boyle, Paul R. Cieslak, John Dunn, Sarah Lathrop, Suzanne Mcguire, Tamara Rissman, Elaine J. Scallan Walter, Kirk Smith, Melissa Tobin-D'Angelo, Katie Wymore, Joanna Zablotsky Kufel, Beverly J. Wolpert, Robert Tauxe, Daniel C. Payne

Pathology Research and Scholarship

Foodborne illnesses are a substantial and largely preventable public health problem; before 2020 the incidence of most infections transmitted commonly through food had not declined for many years. To evaluate progress toward prevention of foodborne illnesses in the United States, the Foodborne Diseases Active Surveillance Network (FoodNet) of CDC's Emerging Infections Program monitors the incidence of laboratory-diagnosed infections caused by eight pathogens transmitted commonly through food reported by 10 U.S. sites.* FoodNet is a collaboration among CDC, 10 state health departments, the U.S. Department of Agriculture's Food Safety and Inspection Service (USDA-FSIS), and the Food and Drug Administration. This report …

Risk Of Hemolytic Uremic Syndrome Related To Treatment Of Escherichia Coli O157 Infection With Different Antimicrobial Classes, Rajal K. Mody, Robert M. Hoekstra, Magdalena Kendall Scott, John Dunn, Kirk Smith, Melissa Tobin-D'Angelo, Beletshachew Shiferaw, Katie Wymore, Paula Clogher, Amanda Palmer, Nicole Comstock, Kari Burzlaff, Sarah Lathrop, Sharon Hurd, Patricia M. Griffin

Pathology Research and Scholarship

Treatment of Shiga toxin-producing Escherichia coli O157 (O157) diarrhea with antimicrobials might alter the risk of hemolytic uremic syndrome (HUS). However, full characterization of which antimicrobials might affect risk is lacking, particularly among adults. To inform clinical management, we conducted a case-control study of residents of the FoodNet surveillance areas with O157 diarrhea during a 4-year period to assess antimicrobial class-specific associations with HUS among persons with O157 diarrhea. We collected data from medical records and patient interviews. We measured associations between treatment with agents in specific antimicrobial classes during the first week of diarrhea and development of HUS, adjusting …

Mitochondrial Proteins In Mediating Colon Cancer And Potential Theraputics, Kunlun Yin

Chemistry and Chemical Biology ETDs

Cancer is one of the biggest threatens to human health. Many people died because of various types of cancer. Colon cancer is one of the leading causes of new incidence and mortality in the whole world. It is important to understand the mechanism of tumorigenesis and development. Based on the mechanisms, we may develop new therapeutics to improve survival rate. In Chapter 2, we discussed the PINK1 function in suppression colon cancer. We found that PINK1 deficiency can promote colon cancer, and PINK1 overexpression suppressed tumor growth in vivo and in vitro. PINK1 can activate its substrate P53, which activates …

Global Population Divergence Of A Cosmopolian Desert Plant, Victor Ryan Alfaro

Biology ETDs

Genetic and phenotypic variation can have different patterns within a species if it has populations with contrasting histories. Populations can have discrete differences that are shaped by different evolutionary scenarios, but within each population, range, or region, traits and association with fitness can also be affected by both edaphic and landscape variation. For my dissertation, I surveyed and experimentally analyzed variation and adaptive potential in Sahara mustard (Brassica tournefortii), a desert annual that has endemic, invasive, and agricultural populations in Africa, Asia, and the Americas. Although my multi-trait analysis generated complex results, my findings can be applied to …

Long-Term Video Object Detection And Tracking In Collaborative Learning Environments, Sravani Teeparthi

Electrical and Computer Engineering ETDs

Human activity recognition in videos is a challenging problem that has drawn a lot of interest, particularly when the goal requires the analysis of a large video database. The Advancing Out-of-School Learning in Mathematics and Engineering (AOLME) project provides a collaborative learning environment for middle school students to explore mathematics, computer science, and engineering by processing digital images and videos. As part of this project, around 2200 hours of video data were collected for analysis. This data was collected to understand how children learn in situations involving mathematical and programming challenges so as to recognize best teaching practices that support …

Uba2 Variants Underlie A Recognizable Syndrome With Variable Aplasia Cutis Congenita And Ectrodactyly, Rhonda E Schnur, Sairah Yousaf, James Liu, Wendy K Chung, Lindsay Rhodes, Michael Marble, Regina M Zambrano, Nara Sobreira, Parul Jayakar, Mary Ella Pierpont, Matthew J Schultz, Pavel N Pichurin, Rory J Olson, Gail E Graham, Matthew Osmond, Gustavo A Contreras-García, Karina A Campo-Neira, Camilo A Peñaloza-Mantilla, Mark Flage, Srikar Kuppa, Karina Navarro, Maria J Guillen Sacoto, Ingrid M Wentzensen, Maria I Scarano, Jane Juusola, Carlos E Prada, Robert B Hufnagel

Pediatrics Research and Scholarship

PURPOSE: The human chromosome 19q13.11 deletion syndrome is associated with a variable phenotype that includes aplasia cutis congenita (ACC) and ectrodactyly as specific features. UBA2 (ubiquitin-like modifier-activating enzyme 2) lies adjacent to the minimal deletion overlap region. We aimed to define the UBA2-related phenotypic spectrum in humans and zebrafish due to sequence variants and to establish the mechanism of disease.

METHODS: Exome sequencing was used to detect UBA2 sequence variants in 16 subjects in 7 unrelated families. uba2 loss of function was modeled in zebrafish. Effects of human missense variants were assessed in zebrafish rescue experiments.

RESULTS: Seven human UBA2 …

Canonical Nf-Κb Promotes Lung Epithelial Cell Tumour Growth By Downregulating The Metastasis Suppressor Cd82 And Enhancing Epithelial-To-Mesenchymal Cell Transition, Eugenia Roupakia, Evangelia Chavdoula, Georgia Karpathiou, Giannis Vatsellas, Dimitrios Chatzopoulos, Angeliki Mela, Jennifer M. Gillette, Katharina Kriegsmann, Mark Kriegsmann, Anna Batistatou, Anna Goussia, Kenneth B. Marcu, Emmanouil Karteris, Apostolos Klinakis, Evangelos Kolettas

Pathology Research and Scholarship

BACKGROUND: The development of non-small cell lung cancer (NSCLC) involves the progressive accumulation of genetic and epigenetic changes. These include somatic oncogenic

METHODS: Human NSCLC cells were used to knock-down RelA/p65 (RelA/p65

RESULTS: RelA/p65

CONCLUSIONS: Canonical NF-κB signalling promotes NSCLC, in part, by downregulating the metastasis suppressor CD82/KAI1 which inhibits cell migration, EMT and tumour growth.

Wagon Tracks Volume 35, Issue 3 (May 2021)

Wagon Tracks

2 On the Cover: The Trail to Santa Fe by Ron Kil
4 Insights from your President
5 Joanne’s Jottings
2,5-6,9, 15,30 Trail News
7-9 Roots Run Deep on the Santa Fe Trail by Marcia Fox
10-14, 23-20 Diary of William Baskerville, Jr., on the Santa Fe Trail, 1854 by Joy L. Poole
16-17 2021 Symposium
31 In Memoriam: Davy Mitchell, Robbie Roberts
31 Chapter Reports 33 Membership Information
36 American Solar Car Challenge

Towards Creating Smart Cities In Nepal, Ambika P. Adhikari, Keshav Bhattarai

Himalayan Research Papers Archive

Many urban centers in the world are seeking to become smart cities. Nepali city leaders are also aspiring to make their cities smart. A smart city basically has clever improvements made in three sectors of its operations: technological, human, and institutional. Globally, many cities have recently made impressive enhancements in at least one or more of these areas. Nepal’s National Planning Commission (NPC) in 2016 had released a concept paper on smart cities for Nepal, defining smart cities as sustainable, information and technology-based, with high quality services and replicable (NPC 2016). As most Nepali cities still operate with limited infrastructure, …

Longitudinal Assessment Of Cytokine Expression And Plasminogen Activation In Hantavirus Cardiopulmonary Syndrome Reveals Immune Regulatory Dysfunction In End-Stage Disease, Peter Simons, Yan Guo, Virginie Bondu, Susan L. Tigert, Michelle Harkins, Samuel Goodfellow, Cana Tompkins, Devon Chabot-Richards, Xuexian O. Yang, Laura Gonzalez Bosc, Steven Bradfute, Daniel A. Lawrence, Tione Buranda

Pathology Research and Scholarship

Pathogenic New World orthohantaviruses cause hantavirus cardiopulmonary syndrome (HCPS), a severe immunopathogenic disease in humans manifested by pulmonary edema and respiratory distress, with case fatality rates approaching 40%. High levels of inflammatory mediators are present in the lungs and systemic circulation of HCPS patients. Previous studies have provided insights into the pathophysiology of HCPS. However, the longitudinal correlations of innate and adaptive immune responses and disease outcomes remain unresolved. This study analyzed serial immune responses in 13 HCPS cases due to Sin Nombre orthohantavirus (SNV), with 11 severe cases requiring extracorporeal membrane oxygenation (ECMO) treatment and two mild cases. We …