Digital Commons Network^™

An Efficient And Improved Method For Virus-Induced Gene Silencing In Sorghum, Dharmendra Kumar Singh, Hee-Kyung Lee, Ismail M. Dweikat, Kirankumar S. Mysore

Department of Agronomy and Horticulture: Faculty Publications

Background: Although the draft genome of sorghum is available, the understanding of gene function is limited due to the lack of extensive mutant resources. Virus-induced gene silencing (VIGS) is an alternative to mutant resources to study gene function. This study reports an improved and efficient method for Brome mosaic virus (BMV)-based VIGS in sorghum.

Methods: Sorghum plants were rub-inoculated with sap prepared by grinding 2 g of infected Nicotiana benthamiana leaf in 1 ml 10 mM potassium phosphate buffer (pH 6.8) and 100 mg of carborundum abrasive. The sap was rubbed on two to three top leaves of sorghum. Inoculated …

Irrigation Pumps - Preserving Performance, Derrel L. Martin

Department of Agricultural and Biological Systems Engineering: Presentations and White Papers

This paper describes how pumps must be matched to operating conditions and power units to provide the desired performance and efficiency. We have recently seen installations where one or more components are not properly matched causing higher than necessary pumping costs. Inspection and maintenance of systems and measurement of flow and pressure will go a long way toward monitoring if the proper conditions are being met. This paper also covers types of pumps, pump components, pump curves, impeller speed and trim, pump staging, affinity laws, pump drives, power units, cavitation, and net positive suction head.

Detection Of Multi-Tomato Leaf Diseases (Late Blight, Target And Bacterial Spots) In Different Stages By Using A Spectral-Based Sensor, Jinzhu Lu, Reza Ehsani, Yeyin Shi, Ana Isabel De Castro, Shuang Wang

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Several diseases have threatened tomato production in Florida, resulting in large losses, especially in fresh markets. In this study, a high-resolution portable spectral sensor was used to investigate the feasibility of detecting multi-diseased tomato leaves in different stages, including early or asymptomatic stages. One healthy leaf and three diseased tomato leaves (late blight, target and bacterial spots) were defined into four stages (healthy, asymptomatic, early stage and late stage) and collected from a field. Fifty-seven spectral vegetation indices (SVIs) were calculated in accordance with methods published in previous studies and established in this study. Principal component analysis was …

Integration Of Hydrogeophysical Datasets And Empirical Orthogonal Functions For Improved Irrigation Water Management, Catherine E. Finkenbiner, Trenton E. Franz, Justin P. Gibson, Derek M. Heeren, J. D. Luck

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Precision agriculture offers the technologies to manage for infield variability and incorporate variability into irrigation management decisions. The major limitation of this technology often lies in the reconciliation of disparate data sources and the generation of irrigation prescription maps. Here the authors explore the utility of the cosmic-ray neutron probe (CRNP) which measures volumetric soil water content (SWC) in the top ~ 30 cm of the soil profile. The key advantages of CRNP is that the sensor is passive, non-invasive, mobile and soil temperature-invariant, making data collection more compatible with existing farm operations and extending the mapping period. The objectives …

Prediction Of Agricultural Implement Hydraulic Power Requirements Using Controller Area Network Bus Data, Gabriel P. Stoll, Rodney A. Rohrer, Santosh K. Pitla, Joe D. Luck

Department of Agricultural and Biological Systems Engineering: Faculty Publications

One of the important challenges in agricultural machinery research is the ability to effectively determine power requirements of a given field operation. The Controller Area Network (CAN) Bus, also known as ISOBUS, has proven to be an effective digital tool for tractor and implement data collection. This study attempted to determine implement hydraulic power requirements using a combination of existing public tractor CAN messages and minimal added sensors. The sensor signals were published on the CAN bus for ease of simultaneous sensor signal and CAN message data collection. Based upon the available CAN messages, this study attempted to measure hydraulic …

Effects Of Alternate Wetting And Drying Irrigation Regime And Nitrogen Fertilizer On Yield And Nitrogen Use Efficiency Of Irrigated Rice In The Sahel, Koffi Djaman, Valere C. Mel, Lamine Diop, Abdoulaye Sow, Raafat El-Namaky, Baboucarr Manneh, Kazuki Saito, Koichi Futakuchi, Suat Irmak

Department of Agricultural and Biological Systems Engineering: Faculty Publications

The objectives of this study were to investigate water saving strategies in the paddy field and to evaluate the performance of some of the newly released rice varieties. Field experiments were conducted at Fanaye in the Senegal River Valley during two rice growing seasons in 2015. Three irrigation regimes ((i) continuous flooding, (ii) trigging irrigation at soil matric potential (SMP) of 30 kPa, (iii) trigging irrigation at SMP of 60 kPa) were tested in an irrigated lowland rice field. Irrigation regimes (ii) and (iii) are alternate wetting and drying (AWD) cycles. Four inbred rice varieties (NERICA S-21, NERICA S-44, Sahel …

Hydrologic Evaluation Of Residential Rain Gardens Using A Stormwater Runoff Simulator, A. R. Anderson, T. G. Franti, D. P. Shelton

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Engineered bioretention cells with underdrains have shown water quality and hydrologic benefits for abating urban stormwater problems. Less is known about the hydrologic performance of residential rain gardens that rely on in situ soil infiltration as the primary mechanism of volume control. Eleven residential rain gardens in Lincoln, Nebraska, were evaluated using a variable-rate stormwater runoff simulator. A volume-based water quality volume (WQV) design storm of 3.0 cm was applied to each rain garden as an SCS Type II runoff hydrograph until the system began overflowing to test the rain gardens for surface and subsurface storage capacity, drawdown rate, ponding …

Registration For Optical Multimodal Remote Sensing Images Based On Fast Detection,Window Selection, And Histogram Specification, Xiaoyang Zhao, Jian Zhang, Chenghai Yang, Huaibo Song, Yeyin Shi, Xingen Zhou, Dongyan Zhang, Guozhong Zhang

Department of Agricultural and Biological Systems Engineering: Faculty Publications

In recent years, digital frame cameras have been increasingly used for remote sensing applications. However, it is always a challenge to align or register images captured with different cameras or different imaging sensor units. In this research, a novel registration method was proposed. Coarse registration was first applied to approximately align the sensed and reference images. Window selection was then used to reduce the search space and a histogram specification was applied to optimize the grayscale similarity between the images. After comparisons with other commonly-used detectors, the fast corner detector, FAST (Features from Accelerated Segment Test), was selected to extract …

Impact Of Eastern Redcedar Proliferation On Water Resources In The Great Plains Usa—Current State Of Knowledge, Chris B. Zou, Dirac Twidwell, Christine H. Bielski, Dillon T. Fogarty, Aaron R. Mittelstet, Patrick J. Starks, Rodney E. Will, Yu Zhong, Bharat Sharma Acharya

Department of Agricultural and Biological Systems Engineering: Faculty Publications

In the Great Plains of the central United States, water resources for human and aquatic life rely primarily on surface runoff and local recharge from rangelands that are under rapid transformation to woodland by the encroachment of Eastern redcedar (redcedar; Juniperus virginiana) trees. In this synthesis, the current understanding and impact of redcedar encroachment on the water budget and water resources available for non-ecosystem use are reviewed. Existing studies concluded that the conversion from herbaceous-dominated rangeland to redcedar woodland increases precipitation loss to canopy interception and vegetation transpiration. The decrease of soil moisture, particularly for the subsurface soil layer, …

Investigation Of Organic Solvents’ Effects On Kenaf (Hibiscus Cannabinus L.) Biomass Conversion In Subcritical Water, Bahar Meryemoglu, Arif Hasanoglu, Mehtap Kurtulus, Sibel Irmak

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Kenaf biomass was hydrolyzed under subcritical water conditions in the presence of various organic solvents. The solvents tested were tetrahydrofuran (THF), acetone, xylene (mixed isomers) and methanol. The organic compounds released into hydrolysates, total organic contents, water-soluble total phenols, and the molecular weight distributions of the polysaccharides in the hydrolysates, solid residues leftover after hydrolysis and gaseous products formed during the solubilization process were determined. The results showed that organic solvents significantly enhanced the dissolution of kenaf biomass (methanol < (omp)xylene ≤ acetone ~ tetrahydrofuran). The hydrolysis percentage was found to be between 75-82% depending on the type of the solvent. Hydrolysis yield and total organic carbons released into hydrolysates highly differed when the solubilization process was performed under carbon dioxide pressure and this effect considerably varied based on the type of solvent used in hydrolysis process. The main gas product formed during hydrolysis process was carbon dioxide with ~80% composition. Morphological measurements of the solid biomass residues left after hydrolysis showed substantial degradations with increasing number of pores on the biomass surfaces.

Cancerdiscover: An Integrative Pipeline For Cancer Biomarker And Cancer Class Prediction From High-Throughput Sequencing Data, Akram Mohammed, Greyson Biegert, Jiri Adamec, Tomáš Helikar

Department of Biochemistry: Faculty Publications

Accurate identification of cancer biomarkers and classification of cancer type and subtype from High Throughput Sequencing (HTS) data is a challenging problem because it requires manual processing of raw HTS data from various sequencing platforms, quality control, and normalization, which are both tedious and timeconsuming. Machine learning techniques for cancer class prediction and biomarker discovery can hasten cancer detection and significantly improve prognosis. To date, great research efforts have been taken for cancer biomarker identification and cancer class prediction. However, currently available tools and pipelines lack flexibility in data preprocessing, running multiple feature selection methods and learning algorithms, therefore, developing …

Endosomal Escape Of Antisense Oligonucleotides Internalized By Stabilin Receptors Is Regulated By Rab5c And Eea1 During Endosomal Maturation, Colton M. Miller, W. Brad Wan, Punit P. Seth, Edward N. Harris

Department of Biochemistry: Faculty Publications

Second-generation (Gen 2) Antisense oligonucleotides (ASOs) show increased nuclease stability and affinity for their RNA targets, which has translated to improved potency and therapeutic index in the clinic. Gen 2 ASOs are typically modified using the phosphorothioate (PS) backbone modification, which enhances ASO interactions with plasma, cell surface, and intracellular proteins. This facilitates ASO distribution to peripheral tissues and also promotes cellular uptake after injection into animals. Previous work identified that Stabilin receptors specifically internalize PS-ASOs in the sinusoidal endothelial cells of the liver and the spleen. By modulating expression of specific proteins involved in the trafficking and maturation of …

3-O Sulfation Of Heparin Leads To Hepatotropism And Longer Circulatory Half-Life, Colton M. Miller, Yongmei Xu, Katrina M. Kudrna, Blake E. Hass, Brianna M. Kellar, Andrew W. Egger, Jian Liu, Edward N. Harris

Department of Biochemistry: Faculty Publications

Introduction: Heparins are common blood anticoagulants that are critical for many surgical and biomedical procedures used in modern medicine. In contrast to natural heparin derived from porcine gut mucosa, synthetic heparins are homogenous by mass, polymer length, and chemistry.

Materials & methods: Stable cell lines expressing the human and mouse Stabilin receptors were used to evaluate endocytosis of natural and synthetic heparin. We chemoenzymatically produced synthetic heparin consisting of 12 sugars (dodecamers) containing 14 sulfate groups resulting in a non-3-O sulfated structure (n12mer). Half of the n12mer was modified with a 3-O sulfate on a single GlcNS sugar producing the …

Editorial: Structure And Function Of Chloroplasts, Rebecca L. Roston, Juliette Jouhet, Fei Yu, Hongbo Gao

Department of Biochemistry: Faculty Publications

The primary energy resource of life on earth is the sun, whose energy is captured in the form of usable carbons by a process called photosynthesis. Photosynthesis occurs within a cellular organelle adapted to that purpose, called the chloroplast. Chloroplasts are unique metabolic and sensory organelles restricted to plants, algae, and a few protists. In this special topic, we aimed to gather new research, hypotheses, and reviews that would help us to better understand the important role of chloroplasts in all photosynthetic organisms. We were fortunate enough to have submissions from many talented chloroplast researchers. This topic contains a total …

Identification Of Genes Encoding Enzymes Catalyzing The Early Steps Of Carrot Polyacetylene Biosynthesis, Lucas Busta, Won Cheol Yim, Evan William Labrant, Peng Wang, Lindsey Grimes, Kiah Malyszka, John C. Cushman, Patricia Santos, Dylan K. Kosma, Edgar B. Cahoon

Department of Biochemistry: Faculty Publications

Polyacetylenic lipids accumulate in various Apiaceae species after pathogen attack, suggesting that these compounds are naturally occurring pesticides and potentially valuable resources for crop improvement. These compounds also promote human health and slow tumor growth. Even though polyacetylenic lipids were discovered decades ago, the biosynthetic pathway underlying their production is largely unknown. To begin filling this gap and ultimately enable polyacetylene engineering, we studied polyacetylenes and their biosynthesis in the major Apiaceae crop carrot (Daucus carota subsp. sativus). Using gas chromatography and mass spectrometry, we identified three known polyacetylenes and assigned provisional structures to two novel polyacetylenes. We …

Ecoevo-Maps: An Ecology And Evolution Assessment For Introductory Through Advanced Undergraduates, Mindi M. Summers, Brian A. Couch, Jennifer K. Knight, Sara E. Brownell, Alison J. Crowe, Katharine Semsar, Christian D. Wright, Michelle K. Smith

School of Biological Sciences: Faculty Publications

A new assessment tool, Ecology and Evolution–Measuring Achievement and Progression in Science or EcoEvo-MAPS, measures student thinking in ecology and evolution during an undergraduate course of study. EcoEvo-MAPS targets foundational concepts in ecology and evolution and uses a novel approach that asks students to evaluate a series of predictions, conclusions, or interpretations as likely or unlikely to be true given a specific scenario. We collected evidence of validity and reliability for EcoEvo-MAPS through an iterative process of faculty review, student interviews, and analyses of assessment data from more than 3000 students at 34 associate’s-, bachelor’s-, master’s-, and doctoral-granting institutions. The …

Mitochondrial Dysfunction And Infection Generate Immunity–Fecundity Tradeoffs In Drosophila, Justin L. Buchanan, Colin D. Meiklejohn, Kristi L. Montooth

School of Biological Sciences: Faculty Publications

Physiological responses to short-term environmental stressors, such as infection, can have long-term consequences for fitness, particularly if the responses are inappropriate or nutrient resources are limited. Genetic variation affecting energy acquisition, storage, and usage can limit cellular energy availability and may influence resourceallocation tradeoffs even when environmental nutrients are plentiful. Here, we utilized Drosophila mitochondrial– nuclear genotypes to test whether disrupted mitochondrial function interferes with nutrient-sensing pathways, and whether this disruption has consequences for tradeoffs between immunity and fecundity. We found that an energetically-compromised genotype was relatively resistant to rapamycin—a drug that targets nutrient-sensing pathways and mimics resource limitation. Dietary …

Time-Lagged Effects Of Weather On Plant Demography: Drought And Astragalus Scaphoides, Brigitte Tenhumberg, Elizabeth E. Crone, Satu Ramula, Andrew J. Tyre

School of Biological Sciences: Faculty Publications

Temperature and precipitation determine the conditions where plant species can occur. Despite their significance, to date, surprisingly few demographic field studies have considered the effects of abiotic drivers. This is problematic because anticipating the effect of global climate change on plant population viability requires understanding how weather variables affect population dynamics. One possible reason for omitting the effect of weather variables in demographic studies is the difficulty in detecting tight associations between vital rates and environmental drivers. In this paper, we applied Functional Linear Models (FLMs) to long-term demographic data of the perennial wildflower, Astragalus scaphoides, and explored sensitivity of …

Near Infrared Fluorescent Single Walled Carbon Nanotubes As Tissue Localizable Biosensors, Nicole M. Iverson, Michael S. Strano, Nigel F. Strano, Thomas P. Mcnicholas

Department of Agricultural and Biological Systems Engineering: Faculty Publications

A nanosensor for detecting an analyte can include a sub strate, a photoluminescent nanostructure, and a polymer interacting with the photoluminescent nanostructure. The nanosensor can be used in in vivo for biomedical applications.

Impact Of Vegetative Treatment Systems On Multiple Measures Of Antibiotic Resistance In Agricultural Wastewater, Lisa M. Durso, Daniel N. Miller, Christopher G. Henry

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Wastewater is an important vector of antibiotic resistant bacteria and antibiotic resistance genes (ARB/G). While there is broad agreement that ARB/G from agricultural (ag) wastewaters can be transported through the environment and may contribute to untreatable infectious disease in humans and animals, there remain large knowledge gaps surrounding applied details on the types and amounts of ARB/G associated with different agricultural wastewater treatment options and different ag production systems. This study evaluates a vegetative treatment system (VTS) built to treat the wastewater from a beef cattle feedlot. Samples were collected for three years, and plated on multiple media types to …

Special Issue: Microbial Community Modeling: Prediction Of Microbial Interactions And Community Dynamics, Hyun-Seob Song

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Microbial communities are networks of species, the interaction structure of which dynamically reorganizes in a varying environment. Even in a static condition, community dynamics are often difficult to predict due to highly nonlinear interspecies interactions. Understanding the fundamental principles of microbial interactions is therefore key for predicting and harnessing community function and properties. As extensively reviewed previously, mathematical models and computational methods that can predictively link interactions to community behaviors are indispensable tools for achieving this goal [1].

Synthesis, Secretion, And Perception Of Abscisic Acid Regulates Stress Responses In Chlorella Sorokiniana, Maya Khasin, Rebecca E. Cahoon, Sophie Alvarez, Richard Beckeris, Seong-Il Eyun, Qidong Jia, Jean-Jack Riethoven, Kenneth Nickerson, Wayne R. Riekhof

Kenneth Nickerson Papers

Abscisic acid (ABA) is a phytohormone that has been extensively characterized in higher plants for its roles in seed and bud dormancy, leaf abscission, and stress responses. Genomic studies have identified orthologs for ABA-related genes throughout the Viridiplantae, including in unicellular algae; however, the role of ABA in algal physiology has not been characterized, and the existence of such a role has been a matter of dispute. In this study, we demonstrate that ABA is involved in regulating algal stress responses. Chlorella sorokiniana strain UTEX 1230 contains genes orthologous to those of higher plants which are essential for ABA …

Neonchocotyle Violantei N. Sp. (Monogenea, Hexabothriidae) From Pseudobatos Lentiginosus (Rhinopristiformes, Rhinobatidae) Of Yucatán, Gulf Of Mexico = Neonchocotyle Violantei N. Sp. (Monogenea, Hexabothriidae) Do Pseudobatos Lentiginosus (Rhinopristiformes, Rhinobatidae) De Yucatán, Golfo Do México, Guadalupe Quiterio-Rendon, Scott Monks, Griselda Pulido-Flores

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Abstract

Neonchocotyle violantei n. sp. (Monogenea: Hexabothriidae) infects the gill of the Atlantic guitarfish, Pseudobatos lentiginosus (Rhinopristiformes, Rhinobatidae) from littoral waters of Celestún, Yucatán, Mexico. It is assigned to Neonchocotyle because it has, among other features, an asymmetrical haptor, a seminal receptacle, a smooth oötype, and an egg with two elongate filaments. It differs from Neonchocotyle pastinacae, the only congener, by having a small body (821 long by 315 wide, length to width = 2.6:1), two pairs of microhooks between the haptoral appendix suckers, extracaecal (submarginal) vaginal pores, and 5-9 testes. This is the first record of a species …

Diversity And Phylogenetic Relationships Of European Species Of Crepidostomum Braun, 1900 (Trematoda: Allocreadiidae) Based On Rdna, With Special Reference To Crepidostomum Oschmarini Zhokhov & Pugacheva, 1998, Romualda Petkevičiūtė, Virmantas Stunžėnas, Alexander E. Zhokhov, Larisa G. Poddubnaya, Gražina Stanevičiūtė

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Background

Within the genus Crepidostomum Braun, 1900, identification of species and taxonomic decisions made only on the basis of adult morphology have resulted in great problems associated with evaluating actual diversity and validity of species. Life cycle data, while equal in importance to adult characters, are scarce, controversial or incomplete for most Crepidostomum spp. In this study, rDNA sequences generated from adult and larval Crepidostomum spp. and some other allocreadiid species were analyzed to reveal the diversity and phylogenetic relationships of the species and their host range. Detailed morphological description based on light microscopy, SEM tegumental surface topography and genetic …

In Vitro Digestion And Characterization Of 2s Albumin And Digestion-Resistant Peptides In Pecan, Jelena Spiric, Stef J. Koppelman, Andre Knulst, Julie A. Nordlee, Steve L. Taylor, Joseph L. Baumert

Department of Food Science and Technology: Faculty Publications

The 2S albumins are one of the major protein families involved in severe food allergic reactions to nuts, seeds, and legumes, thus potentially making these proteins clinically relevant for allergic sensitization and potential diagnostic markers. In this study, we sought to purify native 2S albumin protein from pecan to further characterize this putative allergen. The purified 2S albumin, Car i 1, from pecan was found to be resistant to digestion by pepsin in simulated gastric fluid (SGF) and comparatively stable to proteolysis by trypsin and pancreatin in simulated intestinal fluid (SIF). Digestion of purified Car i 1 in SGF and …

Vulnerability Of Grazing And Confined Livestock In The Northern Great Plains To Projected Mid- And Late-Twenty-First Century Climate, Justin D. Derner, David Briske, Matt Reeves, T. M. Brown-Brandl, Miranda Meehan, Dana M. Blumenthal, William E. Riebsame

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

The Northern Great Plains (NGP) region of the USA—which comprises Montana, Wyoming, Colorado, North Dakota, South Dakota, and Nebraska—is a largely rural area that provides numerous ecosystem services, including livestock products, cultural services, and conservation of biological diversity. The region contains 25% of the Nation’s beef cattle and approximately one-third of the confined beef cattle, as well as the largest remaining native prairie in the US—the Northern Mixedgrass Prairie. With rising atmospheric CO2, the NGP is projected to experience warmer and longer growing seasons, greater climatic variability, and more extreme events (e.g., increased occurrence of large precipitation events). These climatic …

A Behavioral Mechanistic Investigation Of The Role Of 5-Ht1a Receptors In The Mediation Of Rat Maternal Behavior, Xiaonan Li, Xiaojing Ding, Ruiyong Wu, Leilei Chen, Jun Gao, Gang Hu, Ming Li

Department of Psychology: Faculty Publications

Previous work suggests that 5-HT_1A receptors play a special role in rodent maternal aggression, but not in other aspects of maternal care (e.g. pup retrieval and nest building). The present study re-assessed the basic effects of 5-HT_1A activation or blockade on various maternal responses in postpartum female rats. We also examined the possible behavioral mechanisms underlying the maternal effects of 5-HT_1A. Sprague–Dawley mother rats were injected with a 5-HT_1A agonist 8-OH-DPAT (0.1, 0.5 or 1.0 mg/kg, sc), a 5-HT_1A antagonist WAY-101405 (0.1, 0.5 or 1.0 mg/kg, sc) or 0.9% saline solution on postpartum days …

Synbiotics For Improved Human Health: Recent Developments, Challenges, And Opportunities, Janina A. Krumbeck, Jens Walter, Robert W. Hutkins

School of Biological Sciences: Faculty Publications

Research on combining pro- and prebiotics as synbiotics to enhance human and animal health has accelerated in the past 10 years, including many clinical trials that have assessed a diverse range of synbiotic formulations. In this review, we summarize these studies as well as the commercial applications of synbiotics that are available. In particular, we critically assess the claimed health benefits of synbiotic applications and the ecological and therapeutic factors to consider when designing synbiotics and discuss the implications of these concepts for future research in this field.

Non-Targeted Colonization By The Endomycorrhizal Fungus, Serendipita Vermifera, In Three Weeds Typically Co-Occurring With Switchgrass, Prasun Ray, Yingqing Guo, Jaydeep Kolape

Nebraska Center for Biotechnology: Faculty and Staff Publications

Serendipita vermifera (=Sebacina vermifera; isolate MAFF305830) is a mycorrhizal fungus originally isolated from the roots of an Australian orchid that we have previously shown to be beneficial in enhancing biomass yield and drought tolerance in switchgrass, an important bioenergy crop for cellulosic ethanol production in the United States. However, almost nothing is known about how this root-associated fungus proliferates and grows through the soil matrix. Such information is critical to evaluate the possibility of non-target effects, such as unintended spread to weedy plants growing near a colonized switchgrass plant in a field environment. A microcosm experiment was conducted …

Molecular Basis Of Hemoglobin Adaptation In The High-Flying Bar-Headed Goose, Chandrasekhar Natarajan, Agnieszka Jendroszek, Amit Kumar, Roy E. Weber, Jeremy R. H. Tame, Angela Fago, Jay F. Storz

School of Biological Sciences: Faculty Publications

During the adaptive evolution of a particular trait, some selectively fixed mutations may be directly causative and others may be purely compensatory. The relative contribution of these two classes of mutation to adaptive phenotypic evolution depends on the form and prevalence of mutational pleiotropy. To investigate the nature of adaptive substitutions and their pleiotropic effects, we used a protein engineering approach to characterize the molecular basis of hemoglobin (Hb) adaptation in the high-flying bar-headed goose (Anser indicus), a hypoxia-tolerant species renowned for its trans-Himalayan migratory flights. To test the effects of observed substitutions on evolutionarily relevant genetic backgrounds, …