Digital Commons Network^™

Teaching Insetcs: A Four Lesson Plan To Introduce Insects To Kids, Heather Wilkins

Department of Entomology: Distance Master of Science Projects

This teaching guide is meant to be flexible for grades, time, and scope. The lesson plan is designed for grades 2 – 3, but with additional materials can be expounded on for higher grades or simplified for lower grades. It can be used by utilizing all the suggested materials, assignments, and supplements, or can be easily pared down if say you can’t find a book or don’t have internet access for videos. It is a compilation of videos, books, printable pages, interactive activities, and experiments to teach children using fun and interesting methods. It approaches insects with methods for kinetic, …

High School Arthropod Display Case, Daniel Taylor

Department of Entomology: Distance Master of Science Projects

Background: I teach at a high school in Northfield, Minnesota. I love teaching kids science and getting them excited about it. Unfortunately, I currently only teach chemistry and there are almost no elective science classes at our high school. I would love to get students interested in learning about entomology with me or on their own. Few students have ever been exposed to insect in a positive light ever before.

I have had some success at our school with entomological engagement. Each year during the zoology unit I go in to the biology classes as a “guest lecturer” and give …

Gender And Age Of Migration Differences In Mortality Among Older Mexican Americans, Adriana M. Reyes, Marc A. Garcia

Department of Sociology: Faculty Publications

Objectives: Using a gendered life course perspective, we examine whether the relationship between age of migration and mortality is moderated by gender among a cohort of older Mexican-Americans.

Methods: Data from the Hispanic Established Populations for the Epidemiological Study of the Elderly and recently matched mortality data are used to estimate Cox proportional hazard models.

Results: Our findings indicate the relationship between age of migration and mortality is moderated by gender suggesting a more nuanced perspective of the immigrant mortality paradox. Among men, midlife migrants exhibit an 18 percent lower risk of mortality compared to their U.S.-born …

Give Us A Twirl: Male Baton Twirlers’ Embodied Resistance In A Feminized Terrain, Trenton M. Haltom

Department of Sociology: Faculty Publications

There is a persistent perception that baton twirling is only for girls. This insight is not unwarranted because more often than not, audiences only see girls twirling. Baton twirling, however, is not exclusive to girls; boys twirl too.

In this chapter, I explore how the participation of men and boys in the sport of baton twirling exemplifies forms of embodied resistance. In defiance of more masculine and male-dominated sports, men and boys who twirl exhibit embodied resistance in three key ways: through their participation in this feminized sport, by redefining components of twirling to downplay gender, and through choreography involving …

A Red-State View Of Religious Liberty And Nondiscrimination Protection For Lgbt People, Kelsy Burke, Emily Kazyak

Department of Sociology: Faculty Publications

Despite the legalization of same-sex marriage in 2015, two issues remain contentious in national debates about LGBT rights: employment non-discrimination and religious liberty. About half of all states in the U.S. offer protection against workplace discrimination on the basis of sexual orientation and gender identity. Twenty-one states have religious exemption laws that offer protection for people to refuse services based on their religious beliefs. Debates about such religious exemption laws as they relate to LGBT discrimination have become increasingly common. For instance, a Mississippi law (HB 1523)—passed in April 2016 and implemented in October 2017 after a series of court …

Childhood Misfortune And Handgrip Strength Among Black, White, And Hispanic Americans, Natalie R. Smith, Kenneth F. Ferraro, Blakelee R. Kemp, Patricia M. Morton, Sarah A. Mustillo, Jacqueline L. Angel

Department of Sociology: Faculty Publications

Objective: Although early-life insults may affect health, few studies use objective physical measures of adult health. This study investigated whether experiencing misfortune during childhood is associated with handgrip strength (HGS) in later life.

Method: Data on childhood misfortune and adult characteristics from the Health and Retirement Study were used to predict baseline and longitudinal change in HGS among White, Black, and Hispanic American men and women.

Results: Regression analyses revealed that multiple indicators of childhood misfortune were related to HGS at baseline, but the relationships were distinct for men and women. Over the study, having one childhood impairment predicted steeper …

Avoiding The Major Causes Of Death: Does Childhood Misfortune Reduce The Likelihood Of Being Disease Free In Later Life?, Monica M. Williams, Blakelee R. Kemp, Kenneth F. Ferraro, Sarah A. Mustillo

Department of Sociology: Faculty Publications

Objectives: Although previous research reveals the detrimental effects of early misfortune on the development of chronic diseases in later life, few studies have investigated its effects on remaining disease free. This study draws on cumulative inequality theory to investigate whether experiencing childhood misfortune reduces the likelihood of remaining disease free over time.

Method: This study utilizes five waves of data from the Health and Retirement Study to test whether five domains of childhood misfortune predict being disease free at baseline (2004) and developing disease over time (2004–2012).

Results: Respondents reporting risky parental behaviors during childhood were less likely to be …

Partner Congruence On Fertility Intentions And Values: Implications For Birth Outcomes, Karina M. Shreffler, Stacy Tiemeyer, Julia Mcquillan, Arthur L. Greil, Tiffany Spierling

Department of Sociology: Faculty Publications

In heterosexual couples, both partners’ intentions to have a baby (or not) are associated with the likelihood of a subsequent birth, yet most studies only measure women’s intentions. Therefore, little is known about the potential association of couple agreement or disagreement on intentions or on such values as importance of parenthood, career, and leisure and the implications for childbearing. The goal of this article is to assess whether couple-level agreement or disagreement in fertility intentions and values are associated with the likelihood of a subsequent birth. Guided by the Theory of Conjunctural Action, we use couple data from two waves …

Sexual Behaviours Of Adolescents In Creek Town, Cross River State, Nigeria, Kabiru K. Salami, Rowland E. Edet

Department of Sociology: Faculty Publications

Risky sexual behaviors of adolescents in Creek Town have not been fully profiled. This study investigated the sexual behaviors of adolescents and their various dimensions in Creek Town, in Cross River State, Nigeria. This cross-sectional survey design adopted multi-stage sampling procedure to administer a 112-items questionnaire to 422 adolescents, to elicit information on their sexual behaviors and practices. Four focus group discussion sessions were also conducted with in-school and out-of-school adolescents. The mean age of adolescents was 17 years. Majority (65.4%) of the adolescents were sexually active. The mean age at sexual debut was 15 years for both male and …

It Is Not A Cohort Thing: Interrogating The Relationship Between Age, Cohort And Support For The Environment, Philip Schwadel, Erik W. Johnson

Department of Sociology: Faculty Publications

Cohort replacement is one widely implicated, but seldom studied, mechanism of long-term change in public opinion toward environmental protection. A key difficulty in extant research has been empirically distinguishing cohort effects from those of age. Applying recent methodological advances in age– period–cohort models, we examine the disaggregated effects of age, time period, and birth cohort on changes in Americans’ support of federal spending for environmental protection between 1973 and 2016. Results suggest that cohort replacement provides little explanatory power. Instead, we find large age effects, with the young more likely to be pro-environmental in their views, and substantial changes across …

Variation In Reproductive Timing For The Northern Long-Eared Myotis (Myotis Septentrionalis) Across Nebraska, Keith Geluso, Cliff Lemen, Patricia Freeman, Brett R. Andersen, Jeremy A. White, Heather M. Johnson

School of Natural Resources: Faculty Publications

The northern long-eared myotis (Myotis septentrionalis), a federally threatened species, occurs in extreme northern, eastern, and southern Nebraska. These regions vary in climate due to geographic location, topography, and elevation. During a 1-week period in early July 2015, we surveyed bats across the state and observed striking variation in the reproductive status for M. septentrionalis. We examined whether or not growing degree days, an abiotic climatic factor used mainly for agricultural practices, was associated with this reproductive variation in a mammalian species. In early July, we captured only pregnant females in the Pine Ridge region of northwestern Nebraska, …

Isotopic Composition Of Groundwater And Precipitation In Nebraska, Usa, Mikaela L. Cherry, Troy E. Gilmore, Aaron R. Mittelstet, Didier Gastmans, John Gates

School of Natural Resources: Faculty Publications

Groundwater is vital worldwide for water supply, agriculture and industry. Nearly 60% of all water use in Nebraska is from groundwater. Over 90% of groundwater is used for irrigation in Nebraska, which has the largest area of irrigated land in the United States. Many Nebraskans depend on groundwater for drinking water, both from private wells and municipal wells. The sustainability of groundwater resources is dependent on groundwater recharge. The recharge processes, as well as climatic patterns, influence the stable isotope signatures.

Based on weekly samples collected at two monitoring stations managed by the National Atmospheric Deposition Program (NADP), Harvey (2001) …

Engaging Preservice Secondary Science Teachers In An Ngss-Based Energy Lesson: A Nanoscience Context, Deepika Menon, Mary Sajini Devadas

Department of Teaching, Learning, and Teacher Education: Faculty Publications

The new approach to teaching science presented by the Next Generation Science Standards (NGSS) warrants training high-quality science, technology, engineering, and mathematics (STEM) teachers to prepare the future STEM workforce. We share the implementation of an energy lesson using a nanoscience approach, well-aligned with the NGSS vision, in a secondary- STEM-education course for preservice science teachers. First, we engaged preservice teachers in discussions related to alternate sources of energy; this was followed by a case-study approach to illustrate a real-world problem of energy deficiency and solar energy (solar cells using nanoparticles) as one potential solution because it is cost-efficient, clean, …

Urolithin A, A Gut Metabolite, Improves Insulin Sensitivity Through Augmentation Of Mitochondrial Function And Biogenesis, Ashley Mulcahy Toney, Rong Fan, Yibo Xian, Virginia Chaidez, Amanda E. Ramer-Tait, Soonkyu Chung

Department of Nutrition and Health Sciences: Faculty Publications

Objective: Urolithin A (UroA) is a major metabolite of ellagic acid produced following microbial catabolism in the gut. Emerging evidence has suggested that UroA modulates energy metabolism in various cells. However, UroA’s physiological functions related to obesity and insulin resistance remain unclear. Methods: Male mice were intraperitoneally administrated either UroA or dimethyl sulfoxide (vehicle) along with a high-fat diet for 12 weeks. Insulin sensitivity was evaluated via glucose and insulin tolerance tests and acute insulin signaling. The effects of UroA on hepatic triglyceride accumulation, adipocyte size, mitochondrial DNA content, and proinflammatory gene expressions were determined. The impact of UroA on …

Milk-Derived Exosomes And Metabolic Regulation, Janos Zempleni, Sonal Sukreet, Fang Zhou, Di Wu, Ezra Mutai

Department of Nutrition and Health Sciences: Faculty Publications

Exosomes are natural nanoparticles that play an important role in cell-to-cell communication. Communication is achieved through the transfer of cargos, such as microRNAs, from donor to recipient cells and binding of exosomes to cell surface receptors. Exosomes and their cargos are also obtained from dietary sources, such as milk. Exosome and cell glycoproteins are crucial for intestinal uptake. A large fraction of milk exosomes accumulates in the brain, whereas the tissue distribution of microRNA cargos varies among distinct species of microRNA. The fraction of milk exosomes that escapes absorption elicits changes in microbial communities in the gut. Dietary depletion of …

Biological Aspects Of Mountain Pine Beetle In Lodgepole Pine Stands Of Different Densities In Colorado, Usa, Jose Negron

United States Department of Agriculture, Forest Service / University of Nebraska-Lincoln: Faculty Publications

Research Highlights: The biology of mountain pine beetle (MPB), Dendroctonus ponderosae Hopkins, in Colorado’s lodgepole pine forests exhibits similarities and differences to other parts of its range. Brood emergence was not influenced by stand density nor related to tree diameter. The probability of individual tree attack is influenced by stocking and tree size. Findings have implications for understanding MPB as a disturbance agent and for developing management strategies. Background and Objectives: MPB causes extensive tree mortality of lodgepole pine, Pinus contorta Douglas ex Loudon, across the western US and Canada and is probably the most studied bark beetle in North …

Simulating Detection-Censored Movement Records For Home Range Analysis Planning, Lyndsie S. Wszola, Victoria L. Simonsen, Lucía Corral, Christopher J. Chizinski, Joseph J. Fontaine

Nebraska Cooperative Fish and Wildlife Research Unit: Staff Publications

Home range estimation is an important analytical method in applied spatial ecology, yet best practices for addressing the effects of spatial variation in detection probability on home range estimates remain elusive. We introduce the R package “DiagnoseHR,” simulation tools for assessing how variation in detection probability arising from landscape, animal behavior, and methodological processes affects home range inference. We demonstrate the utility of simulation methods for home range analysis planning by comparing bias arising from three home range estimation methods under multiple detection scenarios. We simulated correlated random walks in three landscapes that varied in detection probability and constructed home …

Report Of The N|150 Commission, Donde Ashmos Plowman, William G. Thomas Iii

University of Nebraska-Lincoln Administration: Reports, Papers, Publications, and Presentations

As the campus prepares to mark its 150th year, the University of Nebraska–Lincoln is poised to chart a bold and distinctive course for the next generation. UNL Chancellor Ronnie Green appointed more than 150 stakeholders of the university – faculty, staff, students, alumni, and community members – to the Nebraska Commission of 150 to envision how the university can best serve Nebraska and the world for the next 25 years.

The university’s inaugural charter in 1869 as a land-grant institution offered a sweeping vision for our founding:

To afford the inhabitants of this state with the means of acquiring a …

Efficacy Of A T Cell-Biased Adenovirus Vector As A Zika Virus Vaccine, Brianna L. Bullard, Brigette N. Corder, Matthew J. Gorman, Michael S. Diamond, Eric A. Weaver

Nebraska Center for Virology: Faculty Publications

Zika virus (ZIKV) is a major public health concern due to the risk of congenital Zika syndrome in developing fetuses and Guillain-Barre syndrome in adults. Currently, there are no approved vaccines available to protect against infection. Adenoviruses are safe and highly immunogenic vaccine vectors capable of inducing lasting humoral and cellular immune responses. Here, we developed two Adenovirus (Ad) vectored Zika virus vaccines by inserting a ZIKV prM-E gene expression cassette into human Ad types 4 (Ad4-prM-E) and 5 (Ad5-prM-E). Immune correlates indicate that Ad5-prM-E vaccination induces both an anti-ZIKV antibody and T-cell responses whereas Ad4-prM-E vaccination only induces a …

Seasonality Of Nitrogen Balances In A Mediterranean Climate Watershed, Oregon, Us, Jiajia Lin, Jana E. Compton, Scott G. Leibowitz, George Mueller-Warrant, William Matthews, Stephen H. Schoenholtz, Daniel M. Evans, Rob A. Coulombe

United States Environmental Protection Agency: Publications

We constructed a seasonal nitrogen (N) budget for the year 2008 in the Calapooia River Watershed (CRW), an agriculturally dominated tributary of the Willamette River (Oregon, U.S.) under Mediterranean climate. Synthetic fertilizer application to agricultural land (dominated by grass seed crops) was the source of 90% of total N input to the CRW. Over 70% of the stream N export occurred during the wet winter, the primary time of fertilization and precipitation, and the lowest export occurred in the dry summer. Averaging across all 58 tributary subwatersheds, 19% of annual N inputs were exported by streams, and 41% by crop …

Repeats Of Unusual Size In Plant Mitochondrial Genomes: Identification, Incidence And Evolution, Emily L. Wynn, Alan C. Christensen

School of Biological Sciences: Faculty Publications

Plant mitochondrial genomes have excessive size relative to coding capacity, a low mutation rate in genes and a high rearrangement rate. They also have abundant non-tandem repeats often including pairs of large repeats which cause isomerization of the genome by recombination, and numerous repeats of up to several hundred base pairs that recombine only when the genome is stressed by DNA damaging agents or mutations in DNA repair pathway genes. Early work on mitochondrial genomes led to the suggestion that repeats in the size range from several hundred to a few thousand base pair are underrepresented. The repeats themselves are …

Methanogens: Pushing The Boundaries Of Biology, Nicole R. Buan

Department of Biochemistry: Faculty Publications

Methanogens are anaerobic archaea that grow by producing methane gas. These microbes and their exotic metabolism have inspired decades of microbial physiology research that continues to push the boundary of what we know about how microbes conserve energy to grow. The study of methanogens has helped to elucidate the thermodynamic and bioenergetics basis of life, contributed our understanding of evolution and biodiversity, and has garnered an appreciation for the societal utility of studying trophic interactions between environmental microbes, as methanogens are important in microbial conversion of biogenic carbon into methane, a high-energy fuel. This review discusses the theoretical basis for …

Methanogens: Pushing The Boundaries Of Biology, Nicole R. Buan

Department of Biochemistry: Faculty Publications

Methanogens are anaerobic archaea that grow by producing methane gas. These microbes and their exotic metabolism have inspired decades of microbial physiology research that continues to push the boundary of what we know about how microbes conserve energy to grow. The study of methanogens has helped to elucidate the thermodynamic and bioenergetics basis of life, contributed our understanding of evolution and biodiversity, and has garnered an appreciation for the societal utility of studying trophic interactions between environmental microbes, as methanogens are important in microbial conversion of biogenic carbon into methane, a high-energy fuel. This review discusses the theoretical basis for …

Assessing Responses Of Betula Papyrifera To Climate Variability In A Remnant Population Along The Niobrara River Valley In Nebraska U.S. Through Dendroecological And Remote Sensing Techniques, Evan Bumann, Tala Awada, Brian Wardlow, Michael Hayes, Jane A. Okalebo, C. Helzer, Anastasios Mazis, J. Hiller, Paolo Cherubini

School of Natural Resources: Faculty Publications

Remnant populations of Betula papyrifera have persisted in the Great Plains after the Wisconsin Glaciation along the Niobrara River Valley, Nebraska. Population health has declined in recent years, and has been hypothesized to be due to climate change. We used dendrochronological techniques to assess the response of B. papyrifera to microclimate (1950-2014), and satellite imagery [Landsat 5 TM (1985-2011) and MODIS (2000-2014)] derived NDVI as a proxy for population health. Growing-season streamflow and precipitation were positively correlated with raw and standardized tree-ring widths and basal area increment increase. Increasing winter and spring temperatures were unfavorable for tree growth while increasing …

The Influence Of Depth And Velocity On Age‐0 Scaphirhynchus Sturgeon Prey Consumption: Implications For Aquatic Habitat Restoration, T R. Gemeinhardt, Nathan J.C. Gosch, Anthony P. Civiello, Nathan J. Chrisman, Heather H. Shaughnessy, Tracy L. Brown, James M. Long, Joseph L. Bonneau

United States Army Corps of Engineers, Omaha District

After the pallid sturgeon (Scaphirhynchus albus) was listed as endangered in 1990, a variety of management actions focusing on early life history needs have been implemented to aid species recovery. Given the scarcity of age‐0 pallid sturgeon, managers and scientists have relied on sympatric congeners to evaluate the effectiveness of management actions in the short term; however, increased understanding of habitat requirements for age‐0 Scaphirhynchus sturgeon is still needed to appropriately focus management efforts. Recently, a lack of food‐producing and foraging habitats were proposed as potential limiting factors for pallid sturgeon, and the purpose of this study was to evaluate …

Gene Flow Mediates The Role Of Sex Chromosome Meiotic Drive During Complex Speciation, Colin D. Meiklejohn, Emily L. Landeen, Kathleen E. Gordon, Thomas Rzatkiewicz, Sarah B. Kingan, Anthony J. Geneva, Jeffrey P. Vedanayagam, Christina A. Muirhead, Daniel Garrigan, Daven C. Presgraves, David L. Stern

School of Biological Sciences: Faculty Publications

During speciation, sex chromosomes often accumulate interspecific genetic incompatibilities faster than the rest of the genome. The drive theory posits that sex chromosomes are susceptible to recurrent bouts of meiotic drive and suppression, causing the evolutionary build- up of divergent cryptic sex-linked drive systems and, incidentally, genetic incompatibilities. To assess the role of drive during speciation, we combine high-resolution genetic mapping of X-linked hybrid male sterility with population genomics analyses of divergence and recent gene flow between the fruitfly species, Drosophila mauritiana and D. simulans. Our findings reveal a high density of genetic incompatibilities and a corresponding dearth of gene …

Fire, Grazing And Climate Shape Plant–Grasshopper Interactions In A Tallgrass Prairie, Ellen A.R. Welti, Fan Qiu, Hannah M. Tetreault, Mark Ungerer, Anthony Joern

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

1. Species interactions are integral to ecological community function, and the structure of species interactions has repercussions for the consequences of species extinctions. Few studies have examined the role of environmental factors in controlling species interaction networks across time.

2. We examined variation in plant–grasshopper network structural properties in response to three major grassland drivers: periodic fire, ungulate grazing and climate.

3. We sequenced a plant barcoding gene from extracted grasshopper gut contents to characterize diets of 26 grasshopper species. Resulting grasshopper species’ diets were combined with long‐term plant and grasshopper surveys to assemble plant–grasshopper networks across 13–19 years for …

The Effects Of Sample Storage Conditions On The Microbial Community Composition In Hydraulic Fracturing Produced Water, Daniel Lipus, Amit Vikram, Richard Hammack, Kyle Bibby, Djuna Gulliver

Roman L. Hruska U.S. Meat Animal Research Center: Reports

The petroleum industry has an increasing interest in understanding the microbial communities driving biofouling and biocorrosion in reservoirs, wells, and infrastructure. However, sampling of the relevant produced fluids from subsurface environments for microbiological analyses is often challenged by high liquid pressures, workplace regulations, operator liability concerns, and remote sampling locations. These challenges result in infrequent sampling opportunities and the need to store and preserve the collected samples for several days or weeks. Maintaining a representative microbial community structure from produced fluid samples throughout storage and handling is essential for accurate results of downstream microbial analyses. Currently, no sample handling or …

Molecular Machinery Of Auxin Synthesis,Secretion, And Perception In The Unicellular Chlorophyte Alga Chlorella Sorokiniana Utex 1230, Maya Khasin, Rebecca E. Cahoon, Kenneth Nickerson, Wayne R. Riekhof

School of Biological Sciences: Faculty Publications

Indole-3-acetic acid is a ubiquitous small molecule found in all domains of life. It is the predominant and most active auxin in seed plants, where it coordinates a variety of complex growth and development processes. The potential origin of auxin signaling in algae remains a matter of some controversy. In order to clarify the evolutionary context of algal auxin signaling, we undertook a genomic survey to assess whether auxin acts as a signaling molecule in the emerging model chlorophyte Chlorella sorokiniana UTEX 1230. C. sorokiniana produces the auxin indole-3-acetic acid (IAA), which was present in both the cell pellet and …

International Commission On Trichinellosis: Recommendations For Genotyping Trichinella Muscle Stage Larvae, Edoardo Pozio, Dante Zarlenga

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Being able to identify the species or genotype of Trichinella is of paramount importance not only for epidemiological studies but to better ascertain the source of outbreaks that still occur worldwide. This has become more critical in recent years given the increase in imported meat products and the relationship that wild animals play in the domestic and sylvatic transmission cycles. In contrast to a time when the genus Trichinella was considered monospecific, research in recent years has revealed that the genus consists of 9 species and at least 3 additional genotypes which have yet to be named. Except for a …