Digital Commons Network^™

Considering Augmentative And Alternative Communication Research For Brain-Computer Interface Practice, Kevin M. Pitt, Jonathan S. Brumberg, Adrienne R. Pitt

Department of Special Education and Communication Disorders: Faculty Publications

Purpose: Brain-computer interfaces (BCIs) aim to provide access to augmentative and alternative communication (AAC) devices via brain activity alone. However, while BCI technology is expanding in the laboratory setting, there is minimal incorporation into clinical practice. Building upon established AAC research and clinical best practices may aid the clinical translation of BCI practice, allowing advancements in both fields to be fully leveraged.

Method: A multidisciplinary team developed considerations for how BCI products, practice, and policy may build upon existing AAC research, based upon published reports of existing AAC and BCI procedures.

Outcomes/Benefits: Within each consideration, a review of BCI research …

Fish & Wildlife News: Summer 2019, Matt Trott

Fish and Wildlife News

No abstract provided.

Water Current, Volume 51, No. 1, Summer 2019

Water Current Newsletter

NWC Summer Spotlight: New Research, Events and Impacts

NWC Projects and Programs Are Heating Up

Bioproduction Of Adipic Acid Using Engineered Pseudomonas Putida Kt2440 From Lignin-Derived Aromatics, Howard Willett

Department of Chemical and Biomolecular Engineering: Dissertations, Theses, and Student Research

Current industrial synthesis of adipic acid is nonrenewable and depends on a carcinogenic starting material, benzene. Biocatalysis with an engineered microorganism could turn a renewable feedstock into a value-added chemical such as adipic acid. Here we engineered P. putida KT2440 to transform lignin-derived aromatics, coumarate and ferulate, into adipic acid. Lignin is a recalcitrant plant biopolymer burned for thermal energy. Conversion of lignin into a value-added chemical will improve the efficiency of lignocellulose processing plants. The best performing engineered KT2440 strain produces 2.52 mM adipate at a 9.5% (mole/mole) yield. This was achieved by the genetic insertion of non-natural biosynthetic …

Tailoring Cell-Material Interactions Via Poly(Acrylic Acid) Brushes To Enhance Nonviral Substrate-Mediated Gene Delivery, Amy Mantz

Department of Agricultural and Biological Systems Engineering: Dissertations, Theses, and Student Research

Nonviral gene delivery modifies gene expression by transferring exogenous genetic material into cells and tissues, typically through a bolus of complexes formed by electrostatic interactions between cationic lipid or polymer vectors with negatively charged nucleic acids (e.g. DNA). Although nonviral gene delivery is safer, more cost-effective, and more flexible compared to viral systems, nonviral transfection suffers from low efficiency due to extracellular and intracellular barriers. Much research has focused on tuning physiochemical properties of the complexing vectors to improve transfection, yet the cell-material interface may prove a better platform to immobilize DNA complexes for substrate-mediated delivery (SMD) and modulate the …

Relative Density And Resource Selection Of Urban Red Foxes In Lincoln, Nebraska, Kyle Dougherty

School of Natural Resources: Dissertations, Theses, and Student Research

Since early reports of urban red fox (Vulpes vulpes) populations in Great Britain, red fox populations have been studied in many large cities throughout Europe, North America, and Australia. However, there has been relatively little research conducted in moderately sized North American cities. To further the ecological understanding of red foxes in moderately sized cities, we investigated relative density and resource selection of the urban fox population in Lincoln, Nebraska. We used presence-only data collected from a citizen science project and inhomogeneous point process models to investigate relative density of urban foxes and deployed GPS collars on ten red …

Microbial Transport As Affected By Residue Cover And Manure Application Rate, Lisa M. Durso, John E. Gilley, Dave B. Marx, Chance A. Thayer, Brian L. Woodbury

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Manure is applied to cropland areas with varying surface cover to meet single- or multiple-year crop nutrient requirements. The objectives of this field study were to (1) examine microbial transport following land application of manure to sites with and without wheat residue, (2) compare microbial loads following land application to meet the 0, 1, 2, 4, and 8-year P-based requirements for corn, and (3) evaluate the effects of rainfall simulation run on microbial transport. Manure was added and incorporated by disking plots that were 0.75 m wide by 2.0 m long. Three 30 min simulated rainfall events, separated by 24 …

The Effects Of Economic Factors And Groundwater Policies On The Timing Of Well Drilling, Qianyu Zhang

Department of Agricultural Economics: Dissertations, Theses, and Student Research

Understanding the factors that affect farmers’ irrigation decisions is critical for a better groundwater management. This study addresses the question of how economic, agronomic, and policy variables affect the timing of irrigation adoption in Nebraska. The key contribution of this paper is to identify farmers’ strategic responses to a particular type of policy intervention, a moratorium on well drilling. Our results estimate how farmers respond when a moratorium is announced but not enforced yet, and when neighboring areas implement a moratorium, which we refer as pre-regulation effect and policy spillover effect, respectively. Results show that farmers are more likely to …

In Vivo Human-Like Robotic Phenotyping Of Leaf And Stem Traits In Maize And Sorghum In Greenhouse, Abbas Atefi

Department of Agricultural and Biological Systems Engineering: Dissertations, Theses, and Student Research

In plant phenotyping, the measurement of morphological, physiological and chemical traits of leaves and stems is needed to investigate and monitor the condition of plants. The manual measurement of these properties is time consuming, tedious, error prone, and laborious. The use of robots is a new approach to accomplish such endeavors, which enables automatic monitoring with minimal human intervention. In this study, two plant phenotyping robotic systems were developed to realize automated measurement of plant leaf properties and stem diameter which could reduce the tediousness of data collection compare to manual measurements. The robotic systems comprised of a four degree …

Herbicide Drift Influence On Amaranthus Spp. Herbicide Resistance Evolution, Bruno Canella Vieira

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

The adverse consequences of herbicide drift towards sensitive crops have been extensively reported in the literature. However, no information is available on the consequences of herbicide drift onto weed species inhabiting boundaries of agricultural fields. Exposure to herbicide drift could be detrimental to long-term weed management as several weed species have evolved herbicide resistance after recurrent selection with low herbicide rates. Despite the herbicide drift exposure and its potential implications on resistance evolution and weed management, resistance prone weed species such as Palmer amaranth (Amaranthus palmeri) and waterhemp (Amaranthus tuberculatus) are often neglected and not properly …

Not All Surveillance Data Are Created Equal—A Multi‐Method Dynamic Occupancy Approach To Determine Rabies Elimination From Wildlife, Amy J. Davis, Jordona D. Kirby, Richard B. Chipman, Kathleen M. Nelson, Tatiana Xifara, Colleen T. Webb, Ryan Wallace, Amy T. Gilbert, Kim M. Pepin

United States Department of Agriculture Wildlife Services: Staff Publications

1. A necessary component of elimination programmes for wildlife disease is effective surveillance. The ability to distinguish between disease freedom and non‐detection can mean the difference between a successful elimination campaign and new epizootics. Understanding the contribution of different surveillance methods helps to optimize and better allocate effort and develop more effective surveillance programmes.

2. We evaluated the probability of rabies virus elimination (disease freedom) in an enzootic area with active management using dynamic occupancy modelling of 10 years of raccoon rabies virus (RABV) surveillance data (2006–2015) collected from three states in the eastern United States. We estimated detection probability …

Uncoordinated Maturation Of Developing And Regenerating Postnatal Mammalian Vestibular Hair Cells, Tian Wang, Mamiko Niwa, Zahra N. Sayyid, Davood K. Hosseini, Nicole Pham, Sherri M. Jones, Anthony J. Ricci, Alan G. Cheng

Department of Special Education and Communication Disorders: Faculty Publications

Sensory hair cells are mechanoreceptors required for hearing and balance functions. From embryonic development, hair cells acquire apical stereociliary bundles for mechanosensation, basolateral ion channels that shape receptor potential, and synaptic contacts for conveying information centrally. These key maturation steps are sequential and presumed coupled; however, whether hair cells emerging postnatally mature similarly is unknown. Here, we show that in vivo postnatally generated and regenerated hair cells in the utricle, a vestibular organ detecting linear acceleration, acquired some mature somatic features but hair bundles appeared nonfunctional and short. The utricle consists of two hair cell subtypes with distinct morphological, electrophysiological …

Media And Repository Support Unit, University Of Nebraska–Lincoln Libraries, Annual Report July 2018–June 2019, Deeann Allison, Linnea Fredrickson, Sue Ann Gardner, Richard Graham, Paul Royster, John Wiese, Andrew Cano, Kate Kane, Jennifer L. Thoegersen

Copyright, Fair Use, Scholarly Communication, etc.

Increasingly, libraries are recognizing the importance of providing access to the research output of their universities. In a June 10, 2019, news release from the provosts of the Big Ten Academic Alliance (BTAA) titled “Sustaining Values and Scholarship” (available at https://tinyurl.com/yyu94aa9), they state, “The Big Ten Academic Alliance will continue its advocacy for a sustainable and open ecosystem of publication. . . . Collectively, our institutions’ more than 50,000 faculty are supported by over $10 billion (2017) in research funding, and our institutions have similarly invested significantly in our capacity to further our missions to advance knowledge. Together, we produce …

Expression Of Multi-Domain Lytic Peptide Genes In Transgenic Plants For Disease Resistance, George Biliarski

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Four non-plant multi-domain lytic peptide genes coding for antimicrobial peptides were expressed in Nicotiana benthamiana plants and tested against three fungal pathogens: Sclerotinia sclerotiorum, Rhizoctonia solani, and Pythium sp. Detached-leaf bioassay was performed for the transgenic plants carrying multi-domain lytic peptide constructs and compared with transgenic and wild type control plants. Symptom area of each leaf was measured with high precision using the Compu-Eye software and processed by SAS statistical package. The transgenic lines ORF13 and RSL1 showed substantial resistance to Sclerotinia sclerotiorum infection producing significantly smaller lesion areas compared to control plants. However, these lines were not …

Impact Of Beta-Adrenergic Agonist Supplementation And Heat Stress On The Microbiome And Gastrointestinal Transcriptome Of Sheep, Erin Margaret Duffy

Department of Animal Science: Dissertations, Theses, and Student Research

Improving animal growth and efficiency are critical points of research as the world’s population and demand for agriculture products increase. Therefore, adaptions or changes in the gut are of interest to maximize growth efficiency and wellbeing of livestock. The gastrointestinal tract of the rumen plays many critical roles with the assistance of the associated microbial community. One way to improve animal performance is supplementation of β-adrenergic agonists (β-AA) which are commonly fed to cattle during the last 20-40 days of the finishing period, improving muscle growth by decreasing adipose deposition and increasing muscle accretion. Two β-AA, Ractopamine HCl (β1-AA) and …

Undergraduate Success In Animal Science Courses Based On Demographics, Motivation, And Online Courses, Haylee Anne Lavoie

Department of Animal Science: Dissertations, Theses, and Student Research

A deeper understanding of student demographics, motivation, and outlook of online vs. face to face courses in Animal Science is needed to allow educators to facilitate greater student success. The purpose of this study was to investigate student factors which effected their final grades. The following courses were surveyed at the University of Nebraska- Lincoln Animal Science Department: Animal Products (ASCI 210), Anatomy and Physiology of Domestic Animals (ASCI 240), Introduction to Companion Animals (ASCI 251), Companion Animal Behavior (ASCI 271), Companion Animal Nutrition (ASCI 321), Equine. Nutrition (ASCI 322), Equine Reproduction (ASCI 342), Animal Welfare (ASCI 370), Processed Meats …

Nationalism And Education: A Case Study Of Germany And Japan, Sarah Vrtiska

Honors Program: Senior Projects (Public)

In this piece I ask the question: How has education contributed to the formation or prevention of nationalism in Germany and Japan? In examining this, after defining the standard conceptions of nationalism, I apply these definitions to pre-war and post-war Germany and Japan. Ultimately, I conclude that the goals of education, concepts of national identity that are taught, history curricula, and control of education all historically have the potential to contribute to the rise of nationalism within a country. Based on these fields, I find that although there are similar nationalist trends in both countries during the pre-war period, in …

South American Coccinellidae (Coleoptera), Part Xix: Overview Of Cryptognathini And Systematic Revision Of South American Cryptognatha Mulsant, Guillermo González, Guy A. Hanley, Robert D. Gordon

Insecta Mundi

Genera of Cryptognathini (Coleoptera: Coccinellidae) are discussed and a key to all recognized genera is provided. Cryptognatha is revised, and species of this genus are keyed.

Survival, Fidelity, And Dispersal Of Double-Crested Cormorants On Two Lake Michigan Islands, Christopher R. Ayers, Katie C. Hanson-Dorr, Ken Stromborg, Todd W. Arnold, Jacob S. Ivan, Brian S. Dorr

United States Department of Agriculture Wildlife Services: Staff Publications

Colony fidelity and dispersal can have important consequences on the population dynamics of colonial-nesting birds. We studied survival and inter-colony movements of Double-crested Cormorants (Phalacrocorax auritus; cormorants) nesting at Spider and Pilot islands, located 9 km apart in western Lake Michigan, during 2008–2014. We used live resighting and dead recovery data from both colonies, plus dead recoveries from throughout North America, in a multistate live and dead encounter model to estimate annual survival, inter-colony movements, plus temporary and permanent emigration to unmonitored sites. Annual survival averaged 0.37 (annual process variation, ˆ = 0.07) for hatch-year, 0.78 (ˆ= 0.08) for secondyear, …

Fibrinogen, Factor Xiii And Fibronectin: A Biophysical And Kinetic Characterization Of Their Interactions, Frank Fabian

Department of Chemical and Biomolecular Engineering: Dissertations, Theses, and Student Research

The development of recombinant-based liquid fibrin tissue sealants having enhanced hemostatic and wound healing properties will involve understanding as yet not well characterized interactions between fibrinogen, fibrin (Fbn) factor XIII, thrombin and fibronectin. We study these phenomena in the context of comparing plasma derived fibrinogen to recombinant fibrinogen (rFI) produced in the milk of transgenic cows. An abundance of purified γγ and γγ’ FI subspecies enables detailed study of γγ or γγ’ biomonomer and their respective Fbn biopolymer formation as having different substrate behaviors of activated plasma derived factor XIII (pFXIIIa₂b₂). High pressure size exclusion (HPSEC) …

Use Of Unmanned Aircraft Systems (Uas) And Multispectral Imagery For Quantifying Agricultural Areas Damaged By Wild Pigs, Justin W. Fischer, Kelsey Greiner, Mark W. Lutman, Bryson L. Webber, Kurt C. Vercauteren

United States Department of Agriculture Wildlife Services: Staff Publications

Wild pigs (Sus scrofa) cause extensive damage to agricultural crops, resulting in lost production and income. A major challenge associated with assessing damage to crops is locating and quantifying damaged areas within agricultural fields. We evaluated a novel method using multispectral high-resolution aerial imagery, collected from sensors mounted on unmanned aircraft systems (UAS), and feature extraction techniques to detect and map areas of corn fields damaged by wild pigs in southern Missouri, USA. Damaged areas were extracted from orthomosaics using visible and near-infrared band combinations, an object-based classification approach, and hierarchical learning cycles. To validate estimates we also collected ground …

High‑Throughput Analysis Of Leaf Physiological And Chemical Traits With Vis–Nir–Swir Spectroscopy: A Case Study With A Maize Diversity Panel, Yufeng Ge, Abbas Atefi, Huichun Zhang, Chenyong Miao, Raghuprakash Kastoori Ramamurthy, Brandi Sigmon, Jinliang Yang, James C. Schnable

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Hyperspectral reflectance data in the visible, near infrared and shortwave infrared range (VIS–NIR– SWIR, 400–2500 nm) are commonly used to nondestructively measure plant leaf properties. We investigated the usefulness of VIS–NIR–SWIR as a high-throughput tool to measure six leaf properties of maize plants including chlorophyll content (CHL), leaf water content (LWC), specific leaf area (SLA), nitrogen (N), phosphorus (P), and potassium (K). This assessment was performed using the lines of the maize diversity panel. Data were collected from plants grown in greenhouse condition, as well as in the field under two nitrogen application regimes. Leaf-level hyperspectral data were collected with …

"Flagella Base Model" And "Flagellin Monomer", Brandon Lasalle, Rebecca Roston

3-D Printed Model Structural Files

"Flagella Base Model" and "Flagellin monomer"

Description: This is a teaching model of the proteins that make a bacterial flagella. All models are depicted in space-fill. The Flagellin monomer and the Flagella base can slot together to show protein quaternary structure and filamentous protein assembly.

Printable models are already uploaded to Shapeways.com in the MacroMolecules shop under the names "Flagella Base Model" and "Flagellin monomer".

This model has been printed successfully using these parameters on Shapeways’ laser sintering printer in the following material: Processed Versatile Plastic (Strong & Flexible Plastic).

Model designer: Brandon Lasalle Authors: Brandon Lasalle and Rebecca Roston …

A “Rule-Of-Five” Framework For Models And Modeling To Unify Mathematicians And Biologists And Improve Student Learning, C. Diaz Eaton, H. C. Highlander, K. D. Dahlquist, G. Ledder, M.D. Lamar, R.C. Schugart

Department of Mathematics: Faculty Publications

Despite widespread calls for the incorporation of mathematical modeling into the undergraduate biology curriculum, there is lack of a common understanding around the definition of modeling, which inhibits progress. In this paper, we extend the “Rule-of-Four,” initially used in calculus reform efforts, to a “Rule-of-Five” framework for models and modeling that is inclusive of varying disciplinary definitions of each. This unifying framework allows us to both build on strengths that each discipline and its students bring, but also identify gaps in modeling activities practiced by each discipline. We also discuss benefits to student learning and interdisciplinary collaboration.

Confronting Models With Data: The Challenges Of Estimating Disease Spillover, Paul C. Cross, Diann J. Prosser, Andrew M. Ramey, Ephraim M. Hanks, Kim M. Pepin

United States Department of Agriculture Wildlife Services: Staff Publications

For pathogens known to transmit across host species, strategic investment in disease control requires knowledge about where and when spillover transmission is likely. One approach to estimating spillover is to directly correlate observed spillover events with covariates. An alternative is to mechanistically combine information on host density, distribution and pathogen prevalence to predict where and when spillover events are expected to occur. We use several case studies at the wildlife–livestock disease interface to highlight the challenges, and potential solutions, to estimating spatiotemporal variation in spillover risk. Datasets on multiple host species often do not align in space, time or resolution, …

Life-History Parameters Of Striacosta Albicosta (Lepidoptera: Noctuidae) Under Laboratory Conditions, Débora G. Montezano, Thomas E. Hunt, Alexandre Specht, Priscila M. C. Luz, Julie A. Peterson

Department of Entomology: Faculty Publications

Striacosta albicosta (Smith) is a key pest of maize and dry beans in North America. It has expanded its distribution from the western Great Plains of the United States to the Great Lakes region in the United States and Canada. There has been limited research on the baseline biological aspects of this insect under controlled conditions. The objective of this study was to detail the biological parameters of S. albicosta feeding on an artificial diet under laboratory conditions. Overall survival from neonate to adult at 26.6 ± 1°C was 36.72% and the total developmental time was approximately 110 d. Survival …

Landscape Edges Shape Dispersal And Population Structure Of A Migratory Fish, Mark A. Kaemingk, S. E. Swearer, S. J. Bury, J. S. Shima

Nebraska Cooperative Fish and Wildlife Research Unit: Staff Publications

Many freshwater organisms have a life-history stage that can disperse through seawater. This has obvious benefits for colonization and connectivity of fragmented sub-populations, but requires a physiologically challenging migration across a salinity boundary. We consider the role of landscape boundaries between freshwater and seawater habitats, and evaluate their potential effects on traits and developmental histories of larvae and juveniles (i.e., dispersing life-history stages) of an amphidromous fish, Galaxias maculatus. We sampled juvenile fish on their return to 20 rivers in New Zealand: 10 rivers had abrupt transitions to the sea (i.e., emptying to an open coastline); these were paired …

Estimating Leaf Area Index And Aboveground Biomass Of Grazing Pastures Using Sentinel-1, Sentinel-2 And Landsat Images, Jie Wang, Xiangming Xiao, Rajen Bajgain, Patrick Starks, Jean Steiner, Russell B. Doughty, Qing Chang

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Grassland degradation has accelerated in recent decades in response to increased climate variability and human activity. Rangeland and grassland conditions directly affect forage quality, livestock production, and regional grassland resources. In this study, we examined the potential of integrating synthetic aperture radar (SAR, Sentinel-1) and optical remote sensing (Landsat-8 and Sentinel-2) data to monitor the conditions of a native pasture and an introduced pasture in Oklahoma, USA. Leaf area index (LAI) and aboveground biomass (AGB) were used as indicators of pasture conditions under varying climate and human activities. We estimated the seasonal dynamics of LAI and AGB using Sentinel-1 (S1), …

Typha (Cattail) Invasion In North American Wetlands: Biology, Regional Problems, Impacts, Ecosystem Services, And Management, Sheel Bansal, Shane C. Lishawa, Sue Newman, Brian A. Tangen, Douglas Wilcox, Dennis Albert, Michael J. Anteau, Michael J. Chimney, Ryann L. Cressey, Edward Dekeyser, Kenneth J. Elgersma, Sarah A. Finkelstein, Joanna Freeland, Richard Grosshans, Page E. Klug, Daniel J. Larkin, Beth A. Lawrence, George Linz, Joy Marburger, Gregory Noe, Clint Otto, Nicholas Reo, Jennifer Richards, Curtis Richardson, Leroy Rodgers, Amy J. Schrank, Dan Svedarsky, Steven Travis, Nancy Tuchman, Lisamarie Windham-Myers

United States Department of Agriculture Wildlife Services: Staff Publications

Typha is an iconic wetland plant found worldwide. Hybridization and anthropogenic disturbances have resulted in large increases in Typha abundance in wetland ecosystems throughout North America at a cost to native floral and faunal biodiversity. As demonstrated by three regional case studies, Typha is capable of rapidly colonizing habitats and forming monodominant vegetation stands due to traits such as robust size, rapid growth rate, and rhizomatic expansion. Increased nutrient inputs into wetlands and altered hydrologic regimes are among the principal anthropogenic drivers of Typha invasion. Typha is associated with a wide range of negative ecological impacts to wetland and agricultural …

Open Textbook Project [Poster], Sue Ann Gardner

University of Nebraska-Lincoln Libraries: Presentations

Details of a porject undertaken to collaboratively write and produce an open access parasitology textbook for undergraduate and graduate students. The book will be published by Zea Books at the University of Nebraska-Lincoln in 2020 and be available in English and Spanish both online and print-on-demand through lulu.com.

Co-Executive Editors: Scott L. Gardner and Sue Ann Gardner, University of Nebraska-Lincoln. Publisher: Paul Royster/Zea Books, University of Nebraska-Lincoln. Spanish Translator: Yoanna Esquivel Greenwood, University of Nebraska-Lincoln. Project Coordinator: Sue Ann Gardner.

Project website: Concepts in Animal Parasitology.