Digital Commons Network^™

Control System For Variable-Orifice Mechatronic Spray Nozzle For Regulation Of Application Rate And Droplet Size Spectra, Rodney Rohrer

Department of Agricultural and Biological Systems Engineering: Dissertations, Theses, and Student Research

Traditional fixed-orifice spray nozzles are selected for flow rate and droplet spectra required for a given pesticide application. Although limited variation in flow can be achieved by adjusting system pressure, this can adversely affect spray quality. Other nozzle configurations, such as Pulse Width Modulated (PWM) nozzles or passive variable-orifice designs, are intended to maintain consistent droplet size and spray pattern as flow rate is changed but those too have limitations.

A variable-orifice nozzle modified by Luck (2012) can span multiple droplet spectra and offers independent control of flow and droplet size; however, an advanced control system must be developed to …

Emerging Approaches To Measure Photosynthesis From The Leaf To The Ecosystem, Matthew H. Siebers, Nuria Gomez-Casanovas, Peng Fu, Katherine Meacham-Hensold, Caitlin E. Moore, Carl J. Bernacchi

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Measuring photosynthesis is critical for quantifying and modeling leaf to regional scale productivity of managed and natural ecosystems. This review explores existing and novel advances in photosynthesis measurements that are certain to provide innovative directions in plant science research. First, we address gas exchange approaches from leaf to ecosystem scales. Leaf level gas exchange is a mature method but recent improvements to the user interface and environmental controls of commercial systems have resulted in faster and higher quality data collection. Canopy chamber and micrometeorological methods have also become more standardized tools and have an advanced understanding of ecosystem functioning under …

Participatory On-Farm Research During Graduate School: Challenges And Opportunities, Fernanda Souza Krupek, Carlos Bonini Pires, Gabriela Inveninato Carmona

Department of Entomology: Faculty Publications

Including farmers, growers, and ranchers (hereinafter referred to as producers) as active participants in the development of knowledge, through extension and on-farm research programs, is an effective method of agricultural technology and innovation transfer. The emerging interest in understanding agricultural systems’ complexity has fostered collaborations between producers and land grant universities, particularly across graduate programs focused on applied research. While the concept of on-farm research is not new, the scale and impact of on-farm studies conducted by researchers in partnership with producers are expanding (Kyveryga, 2019). Can master’s and Ph.D. research projects be conducted at the farm scale and generate …

Population Genomics Of Invasive Rodents On Islands: Genetic Consequences Of Colonization And Prospects For Localized Synthetic Gene Drive, Kevin P. Oh, Aaron B. Shiels, Laura Shiels, Dimitri V. Blondel, Karl J. Campbell, J. Royden Saah, Alun L. Lloyd, Paul Q. Thomas, Fred Gould, Zaid Abdo, John R. Godwin, Antoinette J. Piaggio

United States Department of Agriculture Wildlife Services: Staff Publications

Introduced rodent populations pose significant threats worldwide, with particularly severe impacts on islands. Advancements in genome editing have motivated interest in synthetic gene drives that could potentially provide efficient and localized suppression of invasive rodent populations. Application of such technologies will require rigorous population genomic surveys to evaluate population connectivity, taxonomic identification, and to inform design of gene drive localization mechanisms. One proposed approach leverages the predicted shifts in genetic variation that accompany island colonization, wherein founder effects, genetic drift, and island-specific selection are expected to result in locally fixed alleles (LFA) that are variable in neighboring nontarget populations. Engineering …

Phenological Corrections To A Field-Scale, Et-Based Crop Stress Indicator: An Application To Yield Forecasting Across The U.S. Corn Belt, Yang Yang, Martha C. Anderson, Feng Gao, David M. Johnson, Yun Yang, Liang Sun, Wayne Dulaney, Christopher R. Hain, Jason A. Otkin, John Prueger, Tilden P. Meyers, Carl J. Bernacchi, Caitlin E. Moore

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Soil moisture deficiency is a major factor in determining crop yields in water-limited agricultural production regions. Evapotranspiration (ET), which consists of crop water use through transpiration and water loss through direct soil evaporation, is a good indicator of soil moisture availability and vegetation health. ET therefore has been an integral part of many yield estimation efforts. The Evaporative Stress Index (ESI) is an ET-based crop stress indicator that describes temporal anomalies in a normalized evapotranspiration metric as derived from satellite remote sensing. ESI has demonstrated the capacity to explain regional yield variability in water-limited regions. However, its performance in some …

Studies Of The Dutpase Of The Western Corn Rootworm, Carlos A. Riera-Ruiz

School of Biological Sciences: Dissertations, Theses, and Student Research

The western corn rootworm (WCR), Diabrotica virgifera virgifera, is a major corn pest in the United States and Europe. WCR has developed resistance to multiple management strategies, including Cry proteins. Even though the biology and ecology have been thoroughly studied in WCR, their genome and molecular mechanisms are understudied. This work focuses on the ubiquitous enzyme deoxyuridine triphosphatase (dUTPase) encoded by the DUT gene. dUTPase hydrolyzes dUTP into dUMP and pyrophosphate. It contributes to genome stability by keeping the uracil-to-thymine ratio at a certain level. In WCR, two dUTPase isoforms were predicted using transcriptome analyses. These two potential isoforms …

Characterization Of A Novel Glycerol-3-Phosphate Dehydrogenase (Gpd2) In The Alga Chlamydomonas Reinhardtii, Itzela A. Cruz Powell

School of Biological Sciences: Dissertations, Theses, and Student Research

The green alga Chlamydomonas reinhardtii, like many eukaryotic microalgae, accumulates triacylglycerol (TAG) under certain environmental stresses, such as nitrogen deprivation. TAG is of interest because it is an essential precursor for biofuel production. Canonical glycerol-3-phosphate dehydrogenases catalyze the synthesis of glycerol-3-phosphate (G3P), a key precursor for glycerolipid and TAG synthesis in eukaryotes. The C. reinhardtii genome encodes six GPD homologs. Interestingly, GPD2 is a novel multidomain enzyme, consisting of a phosphatase motif fused to a G3P dehydrogenase domain. GPD2 expression is significantly up-regulated under nutrient deprivation or high salinity, coincidental with the accumulation of TAG or glycerol. Conversely, RNA-mediated …

Deliberate Indifference: An Exploration Of The Student Survivor Activism Group Movement, Shyla Kallhoff

Department of Educational Administration: Dissertations, Theses, and Student Research

#MeToo. It’s On Us. End Rape on Campus. #BeTheSwede. Dear UNL. These phrases have united people all over the world to use their voices and speak out about sexual violence. In higher education, these statements empower students to make their voices heard, and simultaneously invoke fear in campus administrators who do not want to be held accountable for the mishandling/lack of Title IX cases. Student survivor activism groups, the subject of this study, have formed at universities around the country and often use similar statements to advocate for changes they feel need to happen. Finding no previous research, it is …

“I Always Felt Like I Belonged:” A Case Study On A First-Generation Focused Student Success Program And Sense Of Belonging, Stephanie Zobac

Department of Educational Administration: Dissertations, Theses, and Student Research

This qualitative case study explored if and how a first-generation focused student success program fostered sense of belonging amongst first-generation college students. Utilizing the theoretical framework of sense of belonging (Strayhorn, 2012), the following research questions guided this study: (1) How do first-generation students experience a first-generation focused student success program? (2) How do first-generation students experience sense of belonging when participating in a first-generation student success program, if at all? (3) How can institutional policies and practices, in the form of a first-generation student success program support the sense of belonging of first-generation students, if at all? Participants included …

Understanding Rumen Microbial Community Structure And Function Towards Decreasing Methane Emissions, Allison L. Knoell

Department of Animal Science: Dissertations, Theses, and Student Research

Resource use is continually being limited due to this growth, therefore, production of high-quality animal protein sources, such as meat and milk, are challenged. The rumen microbiome is extensive and serves to provide several metabolic requirements for the animal for growth. Recently, a significant amount of research is being driven towards understanding the rumen microbiome due to its large effect on metabolic requirements.

A study was conducted to replace alfalfa with nonforage fiber sources in dairy cows. It was determined milk yield and intake are maintained when nonforage fibers replace forage sources, while decreasing methane levels. Water consumption decreased when …

Environmental Impacts Of Overdesign In Small Community Water Resource Recovery Facilities, Andrew Pham

Department of Environmental Engineering: Theses and Other Student Research

Although water resource recovery facilities (WRRFs) reduce environmental impacts related to water quality, their construction and operation result in negative environmental impacts in other categories. Past research into Nebraska WRRFs investigated variables determining energy intensity, opportunities and barriers for energy efficiency improvements, and environmental impacts of the construction and operation phase. This leads to the research question of what design practices can be considered to reduce the environmental impacts.

The Life Cycle Assessment (LCA) methodology was used to evaluate and compare the inventory and environmental impacts of nine small WRRFs, most of which are serving slow growing or declining populations. …

Nitrogen Supply And Physical Disturbance Shapes Arctic Stream Nitrogen Uptake Through Effects On Metabolic Activity, Maria Myrstener, Steven A. Thomas, Reiner Giesler, Ryan A. Sponseller

School of Natural Resources: Faculty Publications

1. Climate change in the Arctic is altering the delivery of nutrients from terrestrial to aquatic ecosystems. The impact of these changes on downstream lakes and rivers is influenced by the capacity of small streams to retain such inputs. Given the potential for nutrient limitation in oligotrophic Arctic streams, biotic demand should be high, unless harsh environmental conditions maintain low biomass standing stocks that limit nutrient uptake capacity.

2. We assessed the drivers of nutrient uptake in two contrasting headwater environments in Arctic Sweden: one stream draining upland tundra and the other draining an alluvial valley with birch forest. At …

On Atrypanius Cretiger (White, 1855) And Atrypanius Punctatellus (Bates, 1872) (Coleoptera: Cerambycidae: Lamiinae: Acanthocinini), Antonio Santos-Silva, Juan Pablo Botero

Insecta Mundi

Atrypanius punctatellus (Bates, 1872) (Coleoptera: Cerambycidae: Lamiinae: Acanthocinini) is for­mally proposed as a junior synonym of A. cretiger (White, 1855). Atrypanius cretiger is redescribed and the type locality is confirmed as Colombia.

Despite the large amount of work on Acanthocinini published in the past fifty years, the tribe still has many problems. This is particularly true with regard to the limits and definition of the genera. Due to the large num­ber of species in this tribe, especially in the Neotropical region (146 genera and 1,138 species – Tavakilian and Chevillotte 2020; Roguet 2021), it is difficult to perceive eventual synonyms, …

First Record Of Palonica Viridia (Ball, 1903) (Hemiptera: Membracidae) In Utah, Lawrence Barringer

Insecta Mundi

Palonica viridia (Hemiptera: Membracidae) is reported from Utah for the first time. Ecological and trapping information is also provided.

The genus Palonica Ball, 1931 contains seven species, four of which occur in the United States (Deitz and Wal­lace 2012; Wallace 2015). Palonica nogalana Ball is found only in Arizona while P. pyramidata (Uhler) and P. tremulata (Ball) have wide distributions across much of the United States and parts of Canada (Deitz and Wallace 2012). Palonica viridia (Ball) ranges west from Arizona, north to British Columbia and east to Texas and New York into Quebec. Presented is the first record of …

A New Species Of Myrmecotypus Pickard-Cambridge Spider (Araneae: Corinnidae: Castianeirinae) From The Bolivian Orocline, Imitating One Of The World’S Most Aggressive Ants, Robert Perger, Gonzalo D. Rubio

Insecta Mundi

A new species of ant-mimicking spider of the subfamily Castianeirinae, Myrmecotypus rubrofemo­ratus Perger and Rubio, new species (Araneae: Corinnidae), is described from the Pre-Andean area of the Bolivian orocline. Adults of M. rubrofemoratus new species resemble the carpenter ant Camponotus femora­tus Forel, 1907, which is considered one of the most aggressive ants in the world.

The Neotropical castianeirine genus Myrmecotypus Pickard-Cambridge is a group of slender, fast-running spi­ders that includes 11 species (World Spider Catalog 2020). Four species are reported from South America and three from Bolivia: M. iguazu Rubio and Arbino, 2009, M. niger Chickering, 1937, and M. …

First Record Of Phaenops Obtusa (Horn) (Coleoptera: Buprestidae) In Massachusetts, Lawrence Barringer

Insecta Mundi

Phaenops obtusa (Coleoptera: Buprestidae) is reported from Massachusetts for the first time. Eco­logical and trapping information is also provided.

The genus Phaenops Dejean, 1833 (Coleoptera: Buprestidae), sometimes treated as a subgenus of Melanophila Eschscholtz (e.g. Bright 1987), contains 15 species in North America, five which occur in the northeast (Nelson et al. 2008; Paiero et al. 2012). Phaenops obtusa (Horn) is primarily distributed in southeastern United States from Louisiana to Florida and South Carolina, with a disjunct occurrence reported from New York (Nelson et al. 2008). Presented here is the first record of P. obtusa from Massachusetts, extending its disjunct …

The Lycaenid Butterfly Fauna (Lepidoptera) Of Cosñipata, Peru: Annotated Checklist, Elevational Patterns, And Rarity, Gerardo Lamas, Michael L. Mcinnis, Robert C. Busby, Robert K. Robbins

Insecta Mundi

Peru’s Cosñipata Region in Cuzco and Madre de Dios Departments is a valley between Manu National Park and the Amarakaeri Communal Reserve that ranges from 400 to 4,000 m elevation. A team of experienced lepidopterists sampled the butterfly fauna of this valley for more than a decade (7,440 field person-hours). We analyze the data for Lycaenidae (Lepidoptera: Papilionoidea), a family for which we have taxonomic expertise. After adding data on the fauna from museums and the literature, we present an annotated checklist of the 340 Lycaenidae species recorded from the Cosñipata Region with notes for each species on the elevations …

A New Genus Cicatrisphaerion, New Species, New Records, And Redescriptions Of Neotropical Cerambycidae (Coleoptera), Steven W. Lingafelter, Roy F. Morris Ii, Frederick W. Skillman Jr., Antonio Santos-Silva

Insecta Mundi

A new genus, Cicatrisphaerion Lingafelter, Morris, Skillman, and Santos-Silva (Coleoptera: Cer­ambycidae), and three new species of the same authorship, C. wappesi from Quintana Roo, Mexico, C. rileyi from Chiapas, Mexico, and Eupogonius wappesi from Quintana Roo, Mexico, are described. New records and clarification on the distribution of Psyrassaforma janzeni Chemsak, 1991 and P. nitida Chemsak, 1991 are provided. Ameriphoderes amoena (Chemsak and Linsley, 1979) is redescribed based on four male specimens and a new country record from Guatemala is documented. New distributional records are provided for Estola flavobasalis Breuning, 1940 (including a new country record for Bolivia) and Estola …

Thyreos Completes Seed Funding From Invest Nebraska, Strategic Angel Investors, Nebraska Combine

The Combine

First paragraph:

Thyreos (https://www.thyreosvaccines.com/), a vaccine company that is developing a novel vaccine platform that protects against a range of herpesviruses, has announced a $750,000 investment round led by Invest Nebraska with participation from other local angel investors in the animal health and veterinary space.

Challenges And Opportunities In Precision Irrigation Decision-Support Systems For Center Pivots, Jingwen Zhang, Kaiyu Guan, Bin Peng, Chongya Jiang, Wang Zhou, Yi Yang, Ming Pan, Trenton Franz, Derek M. Heeren, Daran R. Rudnick, Olufemi P. Abimbola, Hyungsuk Kimm, Kelly Caylor, Stephen Good, Madhu Khanna, John Gates, Yaping Cai

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Irrigation is critical to sustain agricultural productivity in dry or semi-dry environments, and center pivots, due to their versatility and ruggedness, are the most widely used irrigation systems. To effectively use center pivot irrigation systems, producers require tools to support their decision-making on when and how much water to irrigate. However, currently producers make these decisions primarily based on experience and/or limited information of weather. Ineffective use of irrigation systems can lead to overuse of water resources, compromise crop productivity, and directly reduce producers’ economic return as well as bring negative impacts on environmental sustainability. In this paper, we surveyed …

Variants At The Asip Locus Contribute To Coat Color Darkening In Nellore Cattle, Beatriz B. Trigo, Adam T.H Utsunomiya, Alvaro A.A.D. Fortunato, Marco Milanesi, Rafaela B.P. Torrecilha, Harrison Lamb, Loan Nguyen, Elizabeth M. Ross, Ben Hayes, Romulo C.M. Padula, Thayla S. Sussai, Ludmilla B. Zabarez, Rafael S. Cipriano, Maria M.T. Caminhas, Flavia L. Lopes, Cassiano Pelle, Tosso Leeb, Danika Bannasch, Derek Bickhart, Timothy P.L. Smith, Tad S. Sonstegard, Jose F. Garcia, Yuri T. Utsunomiya

Roman L. Hruska U.S. Meat Animal Research Center: Reports

Background: Nellore cattle (Bos indicus) are well-known for their adaptation to warm and humid environments. Hair length and coat color may impact heat tolerance. The Nellore breed has been strongly selected for white coat, but bulls generally exhibit darker hair ranging from light grey to black on the head, neck, hump, and knees. Given the potential contribution of coat color variation to the adaptation of cattle populations to tropical and sub-tropical environments, our aim was to map positional and functional candidate genetic variants associated with darkness of hair coat (DHC) in Nellore bulls. Results: We performed a genome-wide association study …

How Do Vanhorniidae (Hymenoptera) Parasitize Eucnemidae (Coleoptera)?, Jyrki Muona

Insecta Mundi

The relationship between the beetle family Eucnemidae and the parasitic proctotrupoid family Vanhorniidae is discussed. The only proven host for Vanhornia eucnemidarum Crawford in North America is an undetermined species of the genus Isorhipis Boisduval and Lacordaire. In Europe, the only known host for Vanhornia leileri Hedqvist is Hylis cariniceps (Reitter). The biologies of the hosts differ radically and it ap­pears unlikely that they could be parasitized in a typical proctotrupoid fashion in which eggs are placed in or on the host larva. This supports the hypothesis that small Vanhorniidae larvae attach themselves to the newly hatched beetle larvae, before …

Bioaccumulation And Partitioning Of Heavy Metals In Cicindelidia Haemorrhagica In Yellowstone National Park, Monica Gotschall

School of Natural Resources: Dissertations, Theses, and Student Research

The tiger beetle Cicindelidia haemorrhagica (Coleoptera: Cicindelidae) are widely distributed in Yellowstone National Park (YNP) and exclusively living on thermal areas. Heavy metals including arsenic, copper, lead, and selenium are present in these thermal springs, presenting an unique environment for C. haemorrhagica. Therefore, from 2018 – 2020 I sampled adult C. haemorrhagica from YNP and adult C. haemorrhagica from a known population in a salt flat in Idaho not associated with a hot spring and measured heavy metal concentrations. All populations of C. haemorrhagica bioaccumulated heavy metals. Cuticular waxes showed small concentrations of metals indicating that those metals are …

Identification Of A Chlorovirus Pbcv-1 Protein Involved In Degrading The Host Cell Wall During Virus Infection, Irina V. Agarkova, Leslie C. Lane, David D. Dunigan, Cristian F. Quispe, Garry A. Duncan, Elad Milrot, Abraham Minsky, Ahmed Esmael, Jayadri S. Ghosh, James L. Van Etten

Nebraska Center for Virology: Faculty Publications

Chloroviruses are unusual among viruses infecting eukaryotic organisms in that they must, like bacteriophages, penetrate a rigid cell wall to initiate infection. Chlorovirus PBCV-1 infects its host, Chlorella variabilis NC64A by specifically binding to and degrading the cell wall of the host at the point of contact by a virus-packaged enzyme(s). However, PBCV-1 does not use any of the five previously characterized virus-encoded polysaccharide degrading enzymes to digest the Chlorella host cell wall during virus entry because none of the enzymes are packaged in the virion. A search for another PBCV-1-encoded and virion-associated protein identified protein A561L. The fourth domain …

Sorghum Brown Midrib19 (Bmr19) Gene Links Lignin Biosynthesis To Folate Metabolism, Adedayo O. Adeyanju, Scott E. Sattler, Patrick J. Rich, Luis A. Rivera-Burgos, Xiaochen Xu, Gebisa Ejeta

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Genetic analysis of brown midrib sorghum (Sorghum bicolor) mutant lines assembled in our program has previously shown that the mutations fall into four allelic groups, bmr2, bmr6, bmr12 or bmr19. Causal genes for allelic groups bmr2, bmr6 and bmr12, have since been identified. In this report, we provide evidence for the nature of the bmr19 mutation. This was accomplished by introgressing each of the four bmr alleles into nine different genetic backgrounds. Polymorphisms from four resequenced bulks of sorghum introgression lines containing either mutation, relative to those of a resequenced bulk of the nine normal midrib recurrent parent lines, were …

Europium Sulfide Nanoprobes Predict Antiretroviral Drug Delivery Into Hiv-1 Cell And Tissue Reservoirs, Jonathan Herskovitz, Mahmudul Hasan, Jatin Machhi, Insiya Mukadam, Brendan M. Ottemann, James R. Hilaire, Christopher Woldstad, Joellyn Mcmillan, Yutong Liu, Javier Seravalli, Anandakumar Sarella, Howard E. Gendelman, Bhavesh D. Kevadiya

Department of Biochemistry: Faculty Publications

Background: Delivery of long-acting nanoformulated antiretroviral drugs (ARVs) to human immunodeficiency virus type one cell and tissue reservoirs underlies next generation antiretroviral therapeutics. Nanotheranostics, comprised of trackable nanoparticle adjuncts, can facilitate ARV delivery through real-time drug tracking made possible through bioimaging platforms. Methods: To model HIV-1 therapeutic delivery, europium sulfide (EuS) nanoprobes were developed, characterized and then deployed to cells, tissues, and rodents. Tests were performed with nanoformulated rilpivirine (NRPV), a non-nucleoside reverse transcriptase inhibitor (NNRTI) used clinically to suppress or prevent HIV-1 infection. First, CD4+ T cells and monocyte-derived macrophages were EuS-treated with and without endocytic blockers to identify …

Interplay Between Mirnas And Lncrnas: Mode Of Action And Biological Roles In Plant Development And Stress Adaptation, Xianxiang Meng, Aixia Li, Bin Yu, Shengjun Li

School of Biological Sciences: Faculty Publications

Plants employ sophisticated mechanisms to control developmental processes and to cope with environmental changes at transcriptional and post-transcriptional levels. MicroRNAs (miRNAs) and long noncoding RNAs (lncRNAs), two classes of endogenous noncoding RNAs, are key regulators of gene expression in plants. Recent studies have identified the interplay between miRNAs and lncRNAs as a novel regulatory layer of gene expression in plants. On one hand, miRNAs target lncRNAs for the production of phased small interfering RNAs (phasiRNAs). On the other hand, lncRNAs serve as origin of miRNAs or regulate the accumulation or activity of miRNAs at transcription and post-transcriptional levels. Theses lncRNA …

Origin Of Agricultural Plant Pathogens: Diversity And Pathogenicity Of Rhizoctonia Fungi Associated With Native Prairie Grasses In The Sandhills Of Nebraska, Srikanth Kodati, Anthony O. Adesemoye, Gary Y. Yuen, Jerry Volesky, Sydney E. Everhart

Department of Plant Pathology: Faculty Publications

The Sandhills of Nebraska is a complex ecosystem, covering 50,000 km² in central and western Nebraska and predominantly of virgin grassland. Grasslands are the most widespread vegetation in the U.S. and once dominated regions are currently cultivated croplands, so it stands to reason that some of the current plant pathogens of cultivated crops originated from grasslands, particularly soilborne plant pathogens. The anamorphic genus Rhizoctonia includes genetically diverse organisms that are known to be necrotrophic fungal pathogens, saprophytes, mycorrhiza of orchids, and biocontrol agents. This study aimed to evaluate the diversity of Rhizoctonia spp. on four native grasses in the …

A High-Androgen Microenvironment Inhibits Granulosa Cell Proliferation And Alters Cell Identity, Renee M. Mcfee, Sarah M. Romereim, Alexandria P. Snider, Adam F. Summers, William E. Pohlmeier, Scott G. Kurz, Robert A. Cushman, John S. Davis, Jennifer R. Wood, Andrea S. Cupp

School of Veterinary and Biomedical Sciences: Faculty Publications

A naturally occurring bovine model with excess follicular fluid androstenedione (High A4), reduced fertility, and polycystic ovary syndrome (PCOS)-like characteristics has been identified. We hypothesized High A4 granulosa cells (GCs) would exhibit altered cell proliferation and/or steroidogenesis. Microarrays of Control and High A4 GCs combined with Ingenuity Pathway Analysis indicated that High A4 GCs had cell cycle inhibition and increased expression of microRNAs that inhibit cell cycle genes. Granulosa cell culture confirmed that A4 treatment decreased GC proliferation, increased anti-Müllerian hormone, and increased mRNA for CTNNBIP1. Increased CTNNBIP1 prevents CTNNB1 from interacting with members of the WNT signaling pathway thereby …

Early Satellite Cell Communication Creates A Permissive Environment For Long-Term Muscle Growth, Kevin A. Murach, Bailey D. Peck, Robert A. Policastro, Ivan J. Vechetti, Douglas W. Van Pelt, Cory M. Dungan, Lance T. Denes, Xu Fu, Camille R. Brightwell, Gabriel E. Zentner, Esther E. Dupont-Versteegden, Christopher I. Richards, Jeramiah J. Smith, Christopher S. Fry, John J. Mccarthy, Charlotte A. Peterson

Department of Nutrition and Health Sciences: Faculty Publications

Using in vivo muscle stem cell (satellite cell)-specific extracellular vesicle (EV) tracking, satellite cell depletion, in vitro cell culture, and single-cell RNA sequencing, we show satellite cells communicate with other cells in skeletal muscle during mechanical overload. Early satellite cell EV communication primes the muscle milieu for proper long-term extracellular matrix (ECM) deposition and is sufficient to support sustained hypertrophy in adult mice, even in the absence of fusion to muscle fibers. Satellite cells modulate chemokine gene expression across cell types within the first few days of loading, and EV delivery of miR 206 to fibrogenic cells represses Wisp1 expression …