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Lead Poisoning In A Whooping Crane, S. Bret Synder, Michael J. Richard, John P. Thilsted, Roderick C. Drewien, James C. Lewis Jan 1992

Lead Poisoning In A Whooping Crane, S. Bret Synder, Michael J. Richard, John P. Thilsted, Roderick C. Drewien, James C. Lewis

Proceedings of the North American Crane Workshop

The first known incident of lead poisoning in a whooping crane (Grus americana) occurred as the affected bird completed its second migration from Grays Lake National Wildlife Refuge (NWR), Idaho to Bosque del Apache NWR, New Mexico. It fell ill about 4 1/2 weeks after reaching the winter site and was captured. The bird exhibited signs of anorexia and starvation, weakness with drooped wings, greenish watery diarrhea and an unusual gaping of the mouth with abnormal swallowing motions. Blood showed marked polychromasia (>50%) and reticulocytosis (>80%) of RBC's. Serum chemistry values were not diagnostic. A radiograph …


Crane Migration In Northern New Mexico, Dale W. Stahlecker Jan 1992

Crane Migration In Northern New Mexico, Dale W. Stahlecker

Proceedings of the North American Crane Workshop

Greater sandhill cranes (Grus canadensis tabida) and foster-reared whooping cranes (G. americana) were monitored on a 345 km migration between their traditional stopover in southcentral Colorado and their winter grounds in central New Mexico during 4 autumns (1984-87) and 1 spring (1985). Autumn sandhill crane counts totaled 17,363 in 1984, 9,317 in 1985, 29,053 in 1986, and 26,552 in 1987. Peak flights of over 7,000 cranes in 1 day were recorded in both 1986 and 1987. More than 50% of the cranes were counted on just 4 days in 1984, 1986 and 1987. At least 27% …


Nesting Of Greater Sandhill Cranes On Seney National Wildlife Refuge, Richard P. Urbanek, Theodore A. Bookhout Jan 1992

Nesting Of Greater Sandhill Cranes On Seney National Wildlife Refuge, Richard P. Urbanek, Theodore A. Bookhout

Proceedings of the North American Crane Workshop

During 1987, 59 nests of 57 pairs of greater sandhill cranes (Grus canadensis tabida) were located, mainly from the air, on or near the Seney National Wildlife Refuge, Michigan, and 52 nests were ground-checked. Nests were in available palustrine classes without tree canopies. Only 19% were in Sphagnum bogs, in which most nests from other areas of the Upper Peninsula have been found. Cattail (Typha latifolia) marshes, most prevalent in the managed area of the refuge, contabed 44% of the nests, and sedge (Carex spp.) marshes accounted for 37%. Important co-dominant plant species were leatherleaf …


Renesting Of Mississippi Sandhill Cranes In Jackson County, Mississippi 1965–1989, Jacob M. Valentine Jr. Jan 1992

Renesting Of Mississippi Sandhill Cranes In Jackson County, Mississippi 1965–1989, Jacob M. Valentine Jr.

Proceedings of the North American Crane Workshop

Among 118 active nests of Mississippi sandhill cranes (Grus canadensis pulla) in Jackson County, Mississippi, 1%5-1989, 13 were renests. Three chicks from 19 wild and 3 from 3 Patuxent Wildlife Research Center (PWRC) switched eggs hatched in 13 first nests, but 5 died early and 1 after 2 weeks. Ten eggs were dead (infertile or the embryos died) in or at the nest. Two clutches (3 eggs) were destroyed by mammalian predators, 2 eggs were taken for captive propagation, and 1 was pecked and destroyed by the crane pair. Three chicks hatched from 22 wild eggs and 1 …


The Study Of Relatedness And Genetic Diversity In Cranes, George F. Gee, Herbert C. Dessauer, Jonathan Longmire, W. Elwood Briles, Raymond C. Simon Jan 1992

The Study Of Relatedness And Genetic Diversity In Cranes, George F. Gee, Herbert C. Dessauer, Jonathan Longmire, W. Elwood Briles, Raymond C. Simon

Proceedings of the North American Crane Workshop

The U.S. Fish and Wildlife Service (Service) is responsible for recovery of endangered species in the wild and, when necessary, maintenance in captivity. These programs provide an immediate measure of insurance against extinction. A prerequisite inherent in all of these programs is the preservation of enough genetic diversity to maintain a viable population and to maintain the capacity of the population to respond to change. Measures of genetic diversity examine polymorphic genes that are not influenced by selection pressures. Examples of these techniques and those used to determine relatedness are discussed. Studies of genetic diversity, electrophoresis of blood proteins, relatedness, …


Mycobacterium Bovis Infection In North American Elk (Cervus Elaphus), Charles O. Thoen, William J. Quinn, Lyle D. Miller, Larry L. Stackhouse, Bradford F. Newcomb, James M. Ferrell Jan 1992

Mycobacterium Bovis Infection In North American Elk (Cervus Elaphus), Charles O. Thoen, William J. Quinn, Lyle D. Miller, Larry L. Stackhouse, Bradford F. Newcomb, James M. Ferrell

Michigan Bovine Tuberculosis Bibliography and Database

A naturally occurring outbreak of Mycobacterium bovid infection in captive wild elk (wapiti) in Montana was confirmed by mycobacteriologic examination. Twenty-eight of 143 elk responded to M. bovis purified protein derivative (PPD) tuberculin injected intradermally in the cervical region (SCT). The results of comparative cervical tuberculin skin tests conducted within 9 days of SCT revealed greater responses to M. bovis PPD tuberculin than to M. avium PPD tuberculin in 23 of 28 elk responding. At necropsy, several grossly visible tuberculous lesions were observed in the parenchyma of the lung, thoracic lymph nodes, and submandibular lymph nodes. Microscopic examination of appropriately …


A Implementation Plan For The Rainwater Basin Joint Venture, Richard A. Gersib, Kenneth F. Dinan, Jon D. Kauffeld, Michael D. Onnen, P. Joe Gabig, John E. Cornely, Gerald E. Jasmer, Joseph M. Hyland, Kenneth J. Strom Jan 1992

A Implementation Plan For The Rainwater Basin Joint Venture, Richard A. Gersib, Kenneth F. Dinan, Jon D. Kauffeld, Michael D. Onnen, P. Joe Gabig, John E. Cornely, Gerald E. Jasmer, Joseph M. Hyland, Kenneth J. Strom

Nebraska Game and Parks Commission: White Papers, Conference Presentations, and Manuscripts

Nebraska's Rainwater Basin (RWB) wetland area is identified by the North American Waterfowl Management Plan (NAWMP) as a waterfowl habitat area of major concern in North America. The Rainwater Basin area is recognized as the focal point of a spring migration corridor used by millions of ducks and geese annually (Figure 1). This migration corridor is shaped like an hourglass, with the Rainwater Basin and Central Platte River located at the constriction.


Environmental Contaminants Monitoring In Selected Wetlands Of Wyoming [Biologically Active Elements Study], Pedro Ramirez Jr., Joni Armstrong Jan 1992

Environmental Contaminants Monitoring In Selected Wetlands Of Wyoming [Biologically Active Elements Study], Pedro Ramirez Jr., Joni Armstrong

United States Fish and Wildlife Service: Publications

Sediment, water and biota were collected from selected wetlands in Wyoming for the Biologically Active Elements (BAE) Study in 1988, 1989 and 1990 to identify contaminant problems that may warrant further study. This report also will assist in developing a comprehensive contaminants monitoring program for the state and will provide baseline contaminants data for wetland areas. Sites surveyed included areas affected by historical and current mining; wetlands receiving oilfield or oil refinery discharges; wetlands receiving irrigation return flows; stock ponds and natural depressions in areas with seleniferous soils and wetlands influenced by urban runoff. Monitoring is recommended for wetlands receiving …


Contaminant Groundwater Interception - Rma, S. Paul Miller, William L. Murphy Jan 1992

Contaminant Groundwater Interception - Rma, S. Paul Miller, William L. Murphy

United States Army: Publications

Resolution of groundwater contamination is the major environmental concern at most contamination sites. At some sites immediate control is required to prevent further damage. This paper describes a pioneer groundwater interception and treatment system developed and evaluated at a major Army installation.


Nebraska Summary: S128 Massey Ferguson 393, Nebraska Tractor Test Lab Jan 1992

Nebraska Summary: S128 Massey Ferguson 393, Nebraska Tractor Test Lab

Nebraska Tractor Tests

ABOUT THE TEST REPORT AND USE OF THE DATA The test data contained in this report are a tabulation of the results of a series of tests. Due to the restricted format of these pages, only a limited amount of data and not all of the tractor specifications are included. The full OECD report contains usually about 30 pages of data and specifications. The test data were obtained for each tractor under similar conditions and therefore, provide a means of comparison of performance based on a limited set of reported data. EXPLANATION OF THE TEST PROCEDURES Purpose The purpose of …


Nebraska Summary: S127 Massey Ferguson 253, Nebraska Tractor Test Lab Jan 1992

Nebraska Summary: S127 Massey Ferguson 253, Nebraska Tractor Test Lab

Nebraska Tractor Tests

ABOUT THE TEST REPORT AND USE OF THE DATA The test data contained in this report are a tabulation of the results of a series of tests. Due to the restricted format of these pages, only a limited amount of data and not all of the tractor specifications are included. The full OECD report contains usually about 30 pages of data and specifications. The test data were obtained for each tractor under similar conditions and therefore, provide a means of comparison of performance based on a limited set of reported data. EXPLANATION OF THE TEST PROCEDURES Purpose The purpose of …


Nebraska Summary: S129 Massey Ferguson 396, Nebraska Tractor Test Lab Jan 1992

Nebraska Summary: S129 Massey Ferguson 396, Nebraska Tractor Test Lab

Nebraska Tractor Tests

ABOUT THE TEST REPORT AND USE OF THE DATA The test data contained in this report are a tabulation of the results of a series of tests. Due to the restricted format of these pages, only a limited amount of data and not all of the tractor specifications are included. The full OECD report contains usually about 30 pages of data and specifications. The test data were obtained for each tractor under similar conditions and therefore, provide a means of comparison of performance based on a limited set of reported data. EXPLANATION OF THE TEST PROCEDURES Purpose The purpose of …


Methyl Jasmonate Inhibition Of Root Growth And Induction Of A Leaf Protein Are Decreased In An Arabidopsis Thaliana Mutant, Paul E. Staswick, Wenpei Su, Stephen H. Howell Jan 1992

Methyl Jasmonate Inhibition Of Root Growth And Induction Of A Leaf Protein Are Decreased In An Arabidopsis Thaliana Mutant, Paul E. Staswick, Wenpei Su, Stephen H. Howell

Department of Agronomy and Horticulture: Faculty Publications

Jasmonic acid and its methyl ester, methyl jasmonate (MeJA), are plant signaling molecules that affect plant growth and gene expression. Primary root growth of wild-type Arabidopsis thaliana seedlings was inhibited 50% when seedlings were grown on agar medium containing 0.1 μM MeJA. An ethyl methanesulfonate mutant (jar1) with decreased sensitivity to MeJA inhibition of root elongation was isolated and characterized. Genetic data indicated the trait was recessive and controlled by a single Mendelian factor. MeJA induced polypeptides were detected in Arabidopsis leaves by antiserum to a MeJA-inducible vegetative storage protein from soybean. The induction of these proteins by …


Field Worker Exposure To Selected Insecticides Applied To Com Via Center-Pivot Irrigation, Shripat T. Kamble, Matthew E. Byers, John F. Witkowski, Clyde L. Ogg, Gerald W. Echtenkamp Jan 1992

Field Worker Exposure To Selected Insecticides Applied To Com Via Center-Pivot Irrigation, Shripat T. Kamble, Matthew E. Byers, John F. Witkowski, Clyde L. Ogg, Gerald W. Echtenkamp

Department of Entomology: Faculty Publications

Field workerswere monitored for dermal and respiratory exposure to chlorpyrifos (with and without crop oil), carbaryl, and permethrin at reentry intervals of 2, 4, 8, 24, and 48 h after application. Insecticides were applied to R3 stage corn through an overhead center-pivot irrigation system. Dermal exposure was measured by analyzing 18 gauze pads attached to the clothing of workers to represent human body regions. Hand exposure was determined using cotton gloves. Respiratory exposure was determined using portable air samplers equipped with polyurethane foam plugs to trap ambient insecticide residues. Gas liquid chromatography was used to quantify residues of chlorpyrifos and …


Proposed System Of Nomenclature For Biotypes Of Hessian Fly (Diptera: Cecidomyiidae) In North America, F. L. Patterson, John E. Foster, H. W. Ohm, J. H. Hatchett, P. L. Taylor Jan 1992

Proposed System Of Nomenclature For Biotypes Of Hessian Fly (Diptera: Cecidomyiidae) In North America, F. L. Patterson, John E. Foster, H. W. Ohm, J. H. Hatchett, P. L. Taylor

Department of Entomology: Faculty Publications

Twenty genes in wheat, Triticum spp., for resistance to Hessian fly, Mayetiola destructor (Say),have been previously designated H1 to H20. The location on wheat chromosomes of some of the genes is known, but several have not yet been assigned to specific chromosomes. Four wheat differential cultivars have been used to identify 16 possible biotypes of Hessian fly; biotypes were designated GP and A-O. If an additional differential host genotype were added, it is apparent that there are not enough letters in the alphabet to designate all of the biotypes. Therefore, a new system of biotype designation is proposed. Three …


Sensitivity To 2,4-D In Sunflower As An Indicator Of Tolerance To The Sunflower Midge (Diptera: Cecidomyiidae), Marc D. Anderson, Gary J. Brewer Jan 1992

Sensitivity To 2,4-D In Sunflower As An Indicator Of Tolerance To The Sunflower Midge (Diptera: Cecidomyiidae), Marc D. Anderson, Gary J. Brewer

Department of Entomology: Faculty Publications

Nine sunflower hybrids were evaluated for their sensitivity to 2,4-dichlorophenoxyacetic acid (2,4-D) as measured by the production of ethylene. Sensitivity to 2,4-D was then compared with the degree of field tolerance to the sunflower midge, Contarinia schulzi Gagné, exhibited by each hybrid. For the hybrids evaluated, ethylene production increased with 2,4-D concentration and sensitivity to 2,4-D was inversely proportional to midge tolerance. The procedure may be useful in identifying midge tolerant germplasm.


Degradation And Biological Efficacy Of Chlorpyrifos-Methyl On Wheat Stored At Five Temperatures And Three Moisture Contents, Frank H. Arthur, James E. Throne, Richard A. Simonaitis Jan 1992

Degradation And Biological Efficacy Of Chlorpyrifos-Methyl On Wheat Stored At Five Temperatures And Three Moisture Contents, Frank H. Arthur, James E. Throne, Richard A. Simonaitis

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Soft red winter wheat was treated with a calculated dosage of 6 ppm chlorpyrifos-methyl and stored at 15, 20, 25, 30, or 35°C and either 11.2, 12.1, or 13.7% moisture content (15 combinations). Measured residue deposition on the wheat was 4.39 ± 0.57 ppm, a 27.2% reduction from the calculated dosage. Residue degradation was asymptotic at all combinations except 15°C and 11.2 and 12.1% moisture content. Residue loss during the initial months of storage increased with increases in both temperature and moisture content within temperature. Rice weevils, Sitophilus oryzae (L.), did not survive bimonthly bioassays on wheat stored at either …


New Diets For Production Of House Flies And Stable Flies (Diptera: Muscidae) In The Laboratory, Jerome Hogsette Jan 1992

New Diets For Production Of House Flies And Stable Flies (Diptera: Muscidae) In The Laboratory, Jerome Hogsette

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

A diet for rearing the house fly, Musca domestica (L.), was developed from feed constituents available on a year-round basis in Gainesville, FL. The diet, called the Gainesville House Fly Diet, performed as well or better than the Chemical Specialties Manufacturers' Association fly larval medium (CSMA) and can be mixed, bagged, and delivered by a local feed mill within 3 d. By adding pelleted peanut hulls 1:1 by volume, the house fly diet becomes suitable for rearing the stable fly, Stomoxys calcitrans (L.). Use of these diets and the economics involved are discussed further.


Insect, Mite, And Nematode Pests Of Oat, Jaime E. Araya, John E. Foster Jan 1992

Insect, Mite, And Nematode Pests Of Oat, Jaime E. Araya, John E. Foster

Department of Entomology: Faculty Publications

Various arthropods and nematodes cause damage to oat (Avena sativa L. and A. byzantina K. Koch.) plants throughout their life. No stage is free from damage. Crops can be affected from the seedling stage until the grain is harvested. Pests of oat are either polyphagous (damaging a wide range of plants) or oligophagous (feeding on only a few plant species). Few, if any pests, are truly specific to oat crops.

The presence of various pest species in oat fields does not necessarily imply that yield reduction will result. Hundreds of arthropod species feed on oat cultivated in the USA …


Altered Expression Of Adenovirus 12 Dna-Binding Protein But Not Dna Polymerase During Abortive Infection Of Hamster Cells, Lynne A. Lucher, Benjawan Khuntirat, Jiansheng Zhao, Peter C. Angeletti Jan 1992

Altered Expression Of Adenovirus 12 Dna-Binding Protein But Not Dna Polymerase During Abortive Infection Of Hamster Cells, Lynne A. Lucher, Benjawan Khuntirat, Jiansheng Zhao, Peter C. Angeletti

Nebraska Center for Virology: Faculty Publications

Replication of human adenovirus type 12 DNA is blocked in abortively infected baby hamster kidney cells. The activity and accumulation of adenovirus 12 DNA polymerase is equivalent in infected hamster and human cell extracts. However, the accumulation of adenovirus type 12 DNA-binding protein is approximately 120-fold lower in extracts from infected hamster cells when compared to infected permissive human cells. This difference in accumulation is not because of replication of viral DNA during productive infection, since this difference is observed in the presence of hydroxyurea. The DNA-binding protein from infected hamster cells retains the ability to bind denatured DNA-cellulose. An …


Biological Assessment Missouri National Recreational River Jan 1992

Biological Assessment Missouri National Recreational River

United States Army Corps of Engineers, Omaha District

This is in response to your May 21, 1992, letter requesting review and concurrence on a biological assessment prepared to evaluate routine actions of Section 404 and Section 10 of the Clean Water Act along the Missouri National Recreational River. This response has been prepared in coordination with the U.S. Fish and Wildlife Service's (Service) Nebraska Fish and Wildlife Enhancement Office.

This revision of your March 6, 1991, assessment addressed many of the concerns and recommendations that we brought to your attention in our April 2, 1991, letter to Mr. Kenneth Cooper and subsequent meeting on May 6, 1991. However, …


Final Environmental Assessment For Endangered Species Habitat Enhancement/Creation Along Tile Missouri River Main Stem System Jan 1992

Final Environmental Assessment For Endangered Species Habitat Enhancement/Creation Along Tile Missouri River Main Stem System

United States Army Corps of Engineers, Omaha District

The interior least tern (Sterna antillarum) and the piping plover (Charadrius melodus) are federally endangered and threatened species, respectively, which nest on sandbars in the Missouri River. This nesting habitat has been decreasing in past years, at least in part due to vegetative encroachment. Vegetation is no longer regularly scoured from sandbars by heavy spring flows and/or ice, primarily because flows are regulated by the main stem dams. New sandbar creation is uncommon because the river carries less sediment and is no longer meandering along much of its course. Bank erosion still continues to supply sediment …


Jasmonate, Genes, And Fragrant Signals, Paul E. Staswick Jan 1992

Jasmonate, Genes, And Fragrant Signals, Paul E. Staswick

Department of Agronomy and Horticulture: Faculty Publications

Although first recognized for its growth-inhibiting activity about 20 years ago, JA2 and its fragrant methyl ester MeJA (referred to here collectively as jasmonate) are receiving renewed interest as potentially important signaling molecules in plants. This is because jasmonate markedly increases the expression of specific plant genes, some of which are wound responsive. Similarities with fatty acid-derived stress signaling molecules in animals (1) make jasmonate of general biological interest.


Darcy-Weisbach Roughness Coefficients For Gravel And Cobble Surfaces, John E. Gilley, Eugene R. Kottwitz, Gary A. Wieman Jan 1992

Darcy-Weisbach Roughness Coefficients For Gravel And Cobble Surfaces, John E. Gilley, Eugene R. Kottwitz, Gary A. Wieman

Department of Agricultural and Biological Systems Engineering: Presentations and White Papers

A laboratory study is conducted to measure Darcy-Weisbach roughness coefficients for selected gravel and cobble materials. Varying rates of flow are introduced into a flume in which a given size class of gravel or cobble material is securely attached. Roughness coefficients are calculated from measurements of discharge rate and flow velocity. The laboratory data are used to develop regression equations for relating roughness coefficients to surface cover and Reynolds number. Accurate prediction of roughness coefficients for gravel and cobble surfaces will improve our ability to understand and properly model upland flow hydraulics.


Sediment Delivery On Rill And Interrill Areas, John E. Gilley, D. C. Kincaid, W. J. Elliot, J. M. Laflen Jan 1992

Sediment Delivery On Rill And Interrill Areas, John E. Gilley, D. C. Kincaid, W. J. Elliot, J. M. Laflen

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Equations which relate sediment delivery to a power function of flow rate and slope gradient were evaluated in this study. The data used to parameterize the calculations were obtained from sites where crop residues had been removed, and moldboard plowing and disking had occurred. Measurements of sediment delivery resulting from simulated rainfall were obtained from preformed rills and interrill areas. The equations provided reliable sediment delivery estimates for selected soils located throughout the United States. To use the sediment delivery equations, soil-related parameter values must he identified. Multiple regression analyses were performed to relate parameter values used in the equations …


Cultivators For Conservation Tillage, Robert Grisso, Paul J. Jasa, Elbert C. Dickey Jan 1992

Cultivators For Conservation Tillage, Robert Grisso, Paul J. Jasa, Elbert C. Dickey

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Cultivation, a proven weed control methods, should be used primarily to control weeds, rebuild ridges, or aerate poorly drained or crusted soils. Cultivating just to stir the soil usually dries the soil and can result in lower yields during drier cropping conditions. For example, cultivating during warm and windy conditions can result in ¼ to ¾ inches of soil moisture loss.

The main difference between a conservation tillage cultivator and a conventional cultivator is the ability to handle residue and penetrate the soil. By definition, conservation tillage should leave at least 30 percent of the soil surface covered with crop …


Definitions Of Tillage Systems For Corn, J. C. Siemens, Elbert C. Dickey, E. D. Threadgill Jan 1992

Definitions Of Tillage Systems For Corn, J. C. Siemens, Elbert C. Dickey, E. D. Threadgill

Department of Agricultural and Biological Systems Engineering: Faculty Publications

If "tillage" is defined as the mechanical manipulation of soil, it follows, then, that a "tillage system" would be the sequence of soil-manipulation operations performed in producing a crop. Today, however, such a definition is recognized as inadequate. We know, for instance, that the management of non-harvested plant tissue (i.e., residue) affects both crop production and soil erosion, and that field operations in which the soil is not tilled have a marked influence on soil condition.

Therefore, in this publication, a tillage system is the sequence of all operations involved in producing the crop, including soil manipulation, harvesting, chopping or …


Management Of Feedlot Runoff Control Systems, Elbert C. Dickey, Gerald R. Bodman Jan 1992

Management Of Feedlot Runoff Control Systems, Elbert C. Dickey, Gerald R. Bodman

Department of Agricultural and Biological Systems Engineering: Faculty Publications

During recent years, most open feedlot operators have found it desirable and, in some cases, necessary to install runoff control facilities. Even though the primary purpose of feedlot runoff control systems is the prevention of water pollution, many producers have found that good management of these systems also offers limited economic benefits in the form of fertilizer nutrients and supplemental irrigation water. Well managed runoff control systems also reduce weeds, odors, insects and muddy areas at or near feedlot edges. The runoff control system that has proved most satisfactory for all areas of the country is one consisting of clean …


Monoclonal Antibodies Specific For Mercuric Ions, Dwane E. Wylie, Di Lu, Larry D. Carlson, Randy Carlson, K. Funda Babacan, Sheldon M. Schuster, Fred W. Wagner Jan 1992

Monoclonal Antibodies Specific For Mercuric Ions, Dwane E. Wylie, Di Lu, Larry D. Carlson, Randy Carlson, K. Funda Babacan, Sheldon M. Schuster, Fred W. Wagner

Department of Biochemistry: Faculty Publications

Monoclonal antibodies (mAbs) that react with soluble mercuric ions have been produced by i 'ection of BALB/c mice with a hapten-carrier complex designed to maximize exposure of the metal to the immune system. Three hybridomas producing antibodies that reacted with bovine serum albumin (BSA)-glutathione-HgCI, but not with BSAglutathione, were isolated from the spleen of a mouse given multiple iqjections with glutathione-HgCl conjugated to keyhole limpet hemocyanin. Stable subclones were established from two of these antibodies, designated mAb 4A10 and mAb IF10. The binding of both antibodies to immobilized BSAglutathione- HgCl was inhibited by soluble HgCl2, and dissociation constants …


Light Activation Of Maize Phosphoenolpyruvate Carboxylase Protein-Serine Kinase Activity Is Inhibited By Mesophyll And Bundle Sheath-Directed Photosynthesis Inhibitors, Jin-An Jiao, Raymond Chollet Jan 1992

Light Activation Of Maize Phosphoenolpyruvate Carboxylase Protein-Serine Kinase Activity Is Inhibited By Mesophyll And Bundle Sheath-Directed Photosynthesis Inhibitors, Jin-An Jiao, Raymond Chollet

Department of Biochemistry: Faculty Publications

C4 phosphoenolpyruvate carboxylase (PEPC) is post-translationally regulated by reversible phosphorylation of a specific N-terminal seryl residue in response to light/dark transitions of the parent leaf tissue. The protein-serine kinase (PEPC-PK) that phosphorylates/activates this mesophyll-cytoplasm target enzyme is slowly, but strikingly, activated by high light and inactivated in darkness in vivo by a mechanism involving cytoplasmic protein synthesis/degradation as a primary component. In this report, evidence is presented indicating that the inhibition of Calvin cycle activity by a variety of mesophyll (3-(3,4-dichlorophenyl)-1,1-dimethylurea, isocil, methyl viologen) and bundle sheath (DL-glyceraldehyde)-directed photosynthesis inhibitors blocks the light activation of maize (Zea mays …