Digital Commons Network^™

Organic Anion Lithium Ionic Cocrystal Compounds And Compositions, Michael John Zaworotko, Naga Duggirala, Adam John Smith, Roland Douglas Shytle

USF Patents

A cocrystal having the formula LiX·aM, or a solvate or hydrate thereof, wherein X is a conjugate base of an organic acid, M is a neutral organic molecule, and a is from 0.5 to 4, pharmaceutical compositions comprising such cocrystals, cocrystal solvates, or cocrystal hydrates, and methods of preparing such cocrystals, cocrystal solvates, or cocrystal hydrates, and such pharmaceutical compositions.

Iditarod’S Effect On Myotonic Dystrophy Type 1 Disease Progression In A Drosophila Melanogaster Model, Delaney Grace Humphrey

Departmental Honors & Graduate Capstone Projects

Myotonic Dystrophy type 1 (DM1) is caused by CTG-repeat expansion in the DMPK gene, leading to skeletal muscle weakening and wasting. Irisin, a muscle-secreted cytokine released during exercise, promotes muscle growth, repair, and metabolism. Given irisin’s role in promoting muscle growth, we explored how activity-induced Idit expression impacts muscle function in a Drosophila model of DM1. Healthy control (CUG20) and DM1 (CUG250) flies were generated using the UAS-Gal4 system. CUG20 and CUG250 flies underwent a three-week exercise regimen, with the goal of increasing levels of Iditarod protein, the fruit fly homolog of irisin. Following exercise training, muscle function was assessed …

The Effect Of Cellular Glucose Levels On Inotersen-Mediated Target Reduction In In Vitro Cell Models, Andrea Durwin

Honors Scholar Theses

Antisense oligonucleotide (ASO) drugs are a new therapeutic modality to treat rare as well as common diseases. These drugs work to reduce disease by targeting the mRNA of the gene that causes the disease, therefore limiting the proteins that further propagate the signs and symptoms of the disorder. Many people who suffer from more severe diseases, such as Alzheimer’s, Parkinson’s, Duchenne Muscular Dystrophy (DMD), and a wide array of cancers, utilize ASO drugs for treatment, but the drug is also used with those suffering from more common diseases such as diabetes. The efficacy of ASO drugs to silence their mRNA …

Bacterial Microbiomes In Different Depths Of Sand At The Mississippi Gulf Coast, Hays S. Roth

Honors Theses

Microbial communities in sand play essential roles in their ecosystems, yet little is known about how bacterial diversity and function vary across different sand depths. This study investigated bacterial microbiomes at varying depths (0, 10, 20, 30, and 40 cm) on Biloxi East Beach, on the Mississippi Gulf Coast, using 16S rRNA gene sequencing and assays of extracellular enzyme activity. 11,161 amplicon sequence variants (ASVs) were identified across all samples, with the most abundant bacterial phyla being Planctomycetota, Gammaproteobacteria, and Bacteroidota. Depth had a significant impact on bacterial community composition, with surface layers having more diverse bacterial communities. Microbial diversity …

Using The Million Mutation Project Strains Of C. Elegans To Identify Candidate Genes Linked To Abnormal Amphetamine-Induced Behaviors, Nicole Litvinchuk

Harriet L Wilkes Honors College Theses

Amphetamine (Amph) is a psychostimulant drug that increases extracellular dopamine at the dopaminergic synapses, making chronic Amph users liable to addiction. This project aims to identify yet uncharacterized candidate genes within the dopaminergic system and determine their involvement in amphetamine-induced behaviors. In C. elegans, Amph causes a dopaminedependent behavior named Swimming induced paralysis (Swip). We used Swip to screen animals for altered Amph response. To facilitate the identification of genes involved in the mechanism of action of Amph, we utilized C. elegans strains from The Million Mutation Project (MMP) bearing random but well-characterized mutations. Out of all the MMP strains …

Powering Up Clinical Practice: Mastering Clinical Literature Search Techniques In Embase, Evelyn Wang

HSLIC Faculty and Staff Instructional Resources

This workshop presentation, "Powering Up Clinical Practice: Mastering Clinical Literature Search Techniques in EMBASE," is designed to enhance healthcare professionals’ skills in conducting effective clinical literature searches using EMBASE. The session covers efficient navigation of EMBASE, transforming clinical questions into search concepts, developing advanced search strategies with keywords and Emtree terms, applying search tips (Boolean logic, truncation, wildcards), conducting pilot searches, refining search strategies, and managing results for clinical decision-making. Additional resources and HSLIC services are introduced to support literature review and research workflows.

The Extracellular Matrix As A Barrier To C1q Synapse Tagging, Kayla Difede

Harriet L Wilkes Honors College Theses

This project examined C1q’s distribution in the extracellular matrix (ECM) and its specialized structures, perineuronal nets (PNNs), to explore the potential protective role the ECM plays against synaptic pruning. Traditionally, C1q deposits at weak synapses, initiating the complement cascade and signaling microglia to remove them. Preliminary data from the lab show that C1q accumulates in the ECM, suggesting a sequestering mechanism to protect synapses from C1q “tagging” and further elimination. In fact, initial observations indicate that most C1q clusters were not directly associated at the synapse. Using microscopy with fluorescent synaptic markers (Gephyrin-GFP, Homer-GFP), we assessed C1q’s spatial distribution relative …

Pparα Regulates Er–Lipid Droplet Protein Calsyntenin-3Β To Promote Ketogenesis In Hepatocytes, Lauren F. Uchiyama, Alexander Nguyen, Kevin Qian, Liujuan Cui, Khoi T. Pham, Xu Xiao, Yajing Gao, Yuta Shimanaka, Marcus J. Tol, Laurent Vergnes, Karen Reue, Peter Tontonoz

Physiology Faculty Publications

Ketogenesis requires fatty acid flux from intracellular (lipid droplets) and extrahepatic (adipose tissue) lipid stores to hepatocyte mitochondria. However, whether interorganelle contact sites regulate this process is unknown. Recent studies have revealed a role for Calsyntenin-3β (CLSTN3β), an endoplasmic reticulum–lipid droplet contact site protein, in the control of lipid utilization in adipose tissue. Here, we show that Clstn3b expression is induced in the liver by the nuclear receptor PPARα in settings of high lipid utilization, including fasting and ketogenic diet feeding. Hepatocyte-specific loss of CLSTN3β in mice impairs ketogenesis independent of changes in PPARα activation. Conversely, hepatic overexpression of CLSTN3β …

A Mean Field Theory For Pulse-Coupled Neural Oscillators Based On The Spike Time Response Curve, Carmen C. Canavier

School of Medicine Faculty Publications

A mean field method for pulse-coupled oscillators with delays used a self-connected oscillator to represent a synchronous cluster of N - 1 oscillators and a single oscillator assumed to be perturbed from the cluster. A periodic train of biexponential conductance input was divided into a tonic and a phasic component representing the mean field input. A single cycle of the phasic conductance from the cluster was applied to the single oscillator embedded in the tonic component at different phases to measure the change in the cycle length in which the perturbation was initiated, that is, the first-order phase response curve …

Non-Finite Clause Use In Disciplinary Research Writing: A Formulaic Sequence-Based Functional Comparison Between Expert And Student Writers, Xixin Qiu

Faculty Scholarship

Non-finite clauses (NFCs), despite their increasingly recognized role in second language (L2) acquisition and academic writing as part of a multidimensional conceptualization of syntactic complexity, have not been analyzed in functional and discipline-specific perspectives. This study addresses this gap by providing a linguistic-descriptive account of NFC use in expert and advanced student English research writing. Using a 2.26-million-word corpus of published research articles and student manuscripts in Agricultural Science, this study profiles the distribution of NFC subtypes and identifies frequent discoursal functions realized by non-finite verb-centered formulaic sequences. The findings reveal significant differences in NFC use across writer groups, with …

Percutaneous Transluminal Angioplasty And Stenting (Ptas) In Patients With Symptomatic Intracranial Vertebrobasilar Artery Stenosis (Ivbs), Ramtin Pourahmad, Kiarash Saleki, Sina Zoghi, Ramtin Hajibeygi, Hamed Ghorani, Amin Javanbakht, Sina Goodarzi, Parsa Alijanizadeh, Kelly Trinh, Ravi Shastri, Mohammad Ghasemi-Rad

Faculty, Staff and Students Publications

Background: Approximately 20% of all transient ischaemic attacks (TIAs) and ischaemic strokes occur within the posterior circulation, with vertebrobasilar stenosis identified as the cause in roughly 25% of the cases. Studies have shown that about a quarter of these patients have atherosclerotic stenosis of at least 50% of the vertebrobasilar artery. Stenosis has been shown to be associated with an increased risk of 90-day recurrent vertebrobasilar stroke, particularly in the first few weeks, which is significantly higher when compared with patients with stenosis of the anterior circulation. Therefore, aggressive treatment is important for the patient's prognosis. Stenting is emerging as …

Mapping The Developmental Profile Of Ventricular Zone-Derived Neurons In The Human Cerebellum, Anders W Erickson, Henry Tan, Liam D Hendrikse, Jake Millman, Zachary Thomson, Joseph Golser, Omar Khan, Guanyi He, Kathleen Bach, Arpit Suresh Mishra, Janja Kopic, Zeljka Krsnik, Ferechte Encha-Razavi, Giulia Petrilli, Fabien Guimiot, Evelina Silvestri, Kimberly A Aldinger, Michael D Taylor, Kathleen J Millen, Parthiv Haldipur

Faculty, Staff and Students Publications

The cerebellar ventricular zone (VZ) is the primary source of progenitors that generate cerebellar GABAergic neurons, including Purkinje cells (PCs) and interneurons (INs). This study provides detailed characterization of human cerebellar GABAergic neurogenesis using transcriptomic and histopathological analyses and reveals conserved and unique features compared to rodents. We show that the sequential progression of neurogenesis is conserved and occurs before 8 postconception weeks. Notably, PC differentiation occurs in the outer subventricular zone (SVZ), a region absent in the mouse cerebellum. Human PCs are generated during a compact two-week period before the onset of cerebral cortex histogenesis. A subset of human …

Immune Cells And Arrhythmias, Joshua A Keefe, Jian Wang, Jiangping Song, Li Ni, Xander H T Wehrens

Faculty, Staff and Students Publications

Cardiac arrhythmias are a significant cause of morbidity and mortality worldwide. Emerging evidence has demonstrated that resident and infiltrating cardiac immune cells play direct, mechanistic roles in arrhythmia onset and progression. In this review, we provide a comprehensive summary and expert commentary on the role of each immune cell subtype in the pathogenesis of atrial and ventricular arrhythmias.

Towards An Explicit Understanding Of Network Governance In Urban Forestry Management: A Case Study Of Portland (Or), Usa, Quadri Olatunbosun Taiwo, Vivek Shandas

Geography Faculty Publications and Presentations

As the climate continues to warm, and municipal leaders look for cost-effective and timely approaches to urban sustainability, one increasingly sought-out approach is the use of tree canopy to cool neighborhoods. Despite widespread efforts to expand tree canopy in cities, an overwhelming body of evidence suggests that urban green space is declining across the U.S., yet little is known about the factors that propel these changes. Understanding the institutional and governance systems can help identify the opportunities for slow consistent declines. Using social network analysis (SNA) metrics, we examined stakeholder roles in power structures and decision-making processes within Portland, Oregon’s …

Evaluation Of The Impact Of Urban Stream Syndrome On Macroinvertebrate Functional Feeding Diversity And Organic Matter Processing In Streams, Helena Loucas

Longwood Senior Theses

Urbanization causes the degradation of streams described as the Urban Stream Syndrome, resulting in increased contamination, altered stream morphology, and reduced taxonomic richness. Macroinvertebrates vary in their tolerance to the impacts of urbanization, therefore the reduction of taxonomic richness increases the abundance of tolerant species and decreases the abundance of intolerant species. Aquatic macroinvertebrates are specialized feeders, falling into five functional feeding groups which affect ecosystem processes such as the decomposition of organic matter. Through a reduction of intolerant taxa, functional feeding diversity will be reduced because the functional feeding groups are not equally distributed across all tolerance levels. This …

Synthesis Of Organosilicon Substituted Fₓpczn For The Preparation Of Silica-Based Photo-Catalysts, Reagan E. Daly

Seton Hall University Dissertations and Theses (ETDs)

This study began with the synthesis, purification, and catalytic application of hexadeca fluoro phthalocyanine zinc (F₁₆PcZn) derivatives functionalized with organosilane consuming thiol groups. The F₁₆PcZn was synthesized by reacting tetrafluorophthalonitrile (TFPN) with zinc acetate (Zn(OAc)₂) in 1-chloronaphthalene as a solvent, under a controlled heating process at 185°C for 90 minutes. The resulting blue solid was purified via vacuum filtration and recrystallization techniques to remove residual solvents and by-products, yielding pure F₁₆PcZn. The purity of the product was assessed through UV-Vis spectroscopy, MALDI analysis, and a molar extinction coefficient was determined.

Subsequent nucleophilic substitution of the fluorine atoms on F₁₆PcZn with …

Comparing Parasitism And Resistance To Flow In Two Populations Of Elimia Virginica, Morgan G. Hicok, Abbie Tomba, Kate Green

Departmental Honors & Graduate Capstone Projects

Snails are important in freshwater ecosystems as primary consumers and are good indicators of the stability of a habitat. Trematodes are parasitic flatworms that infect freshwater snails and can cause changes in metabolic function and growth. Flow rates and water levels are now more variable due to climate change, increasing stress and dislodgment in the snail population. This study investigates how parasites, shell morphology, and native flow environment influence resistance to disturbance. Elimia populations were sampled for differences in parasitism, shell morphology, and ability to withstand high flow environments in two different rivers. Sample sites at the Potomac River were …

Exploring Bis-Dinitrocarboxyphenyl-Kryptofix-22 Derivatives As Precursors For Heterogeneous Catalysts In Biodiesel Production, Priscilla Agrinsoni

Harriet L Wilkes Honors College Theses

The growing demand for sustainable fuel alternatives has increased interest in biodiesel production. Current methods are costly due to the need for high-purity feedstocks. Alkaline homogeneous catalysts are commonly used due to their low cost and easy accessibility; however, they are non-reusable, produce low-quality glycerol, and have extensive purification requirements. This research investigates the catalytic performance of a bis-dinitrocarboxyphenylkryptofix- 22 derivative. This derivative is referred to as a “linker”. Its catalytic activity was tested through transesterification reactions. It appears to exhibit limited catalytic activity. This research seeks to lay the groundwork for the future development of metal-organic frameworks (MOFs) as …

Assessing The Effects Of Antibody Glycosylation And Conjugation On Fc-Mediated Effector Functions, Lina Wu

Undergraduate Honors Theses

Increasing research has been conducted on antibody drug conjugates (ADCs) for their oncology applications and potential as treatments for immunological diseases. ADCs usually covalently bind a cytotoxic drug (payload) via a linker to a monoclonal antibody, which targets specific antigens expressed on tumors, delivering the drug directly to cancer cells while minimizing off-target effects. In addition to binding antigens on tumor cells, antibodies also interact with immune cells to activate “effector functions”. This activation is largely driven by the binding of Fcγ-receptors (FcγR) to the Fc region of IgG, which connects the humoral immune responses and cell-mediated immune responses. Antibody …

The Central Role Of Endothelial Cells In Various Contexts Of Neuroinflammation, Joseph M. Kagan

Harriet L Wilkes Honors College Theses

This paper aims to summarize the key differences in neuroinflammation across various contexts beyond neurodegenerative diseases, specifically senescence, diet, infection, cancer treatments, and psychostimulant usage. While each of these conditions presents unique inflammatory characteristics, they share core mechanistic themes, particularly the disruption of the blood-brain barrier (BBB). BBB dysfunction serves as a crucial regulatory point, influenced by endothelial cells (ECs) within the neurovascular unit (NVU). ECs play an essential role in neuroinflammation through pathways involving tight junction (TJ) proteins and matrix metalloproteinases (MMPs), which mediate BBB integrity and permeability. Additionally, ECs interact with various cellular components and signaling pathways, further …

The Lived Experiences Of High School Students Serving As Peer Mentors, Heather S. Mitchell

Dissertations

Youth mentoring is an intervention that has demonstrated a tremendous amount of potential in mitigating a variety of struggles experienced by youth (DuBois & Rhodes, 2006; Grossman & Tierney, 1998; Herrera et al., 2008; “Impact Evaluation”, 2009; Karcher, 2009; Karcher, 2010; Kraus & Cleveland, 2016; Lerner et al., 2006; McQuillin et al., 2020). The exploration of the possible benefits to adolescents serving as mentors, especially nontraditional student mentors, has received little attention (Besnoy & McDaniel, 2016; Deane et al., 2018; James et al., 2014; Karcher, 2009; Karcher et al., 2010; Larson et al., 2020; Opara et al., 2023; Quimby et …

Spartan Daily, April 29, 2025, San Jose State University, School Of Journalism And Mass Communications

Spartan Daily, 2025

Volume 164, Issue 38

Expediting Tool-Use Learning: Effects Of Non-Invasive Neurostimulation Of The Left Anterior Supramarginal Gyrus, Kamila Haliru

Honors Thesis

The left anterior supramarginal gyrus (aSMG) has been implicated as an important brain region in tool use. While anodal transcranial Direct Current Stimulation (atDCS) over the primary motor cortex (M1) has been shown to facilitate skilled tool use, the effects of aSMG stimulation remain largely unexplored. Using a chopstick-marble paradigm, this thesis examined whether atDCS to the left aSMG would enhance tool-use learning. Electroencephalography (EEG) was concurrently recorded to assess atDCS-dependent changes to the beta and gamma frequency bands. Our results demonstrated that atDCS over the left aSMG significantly expedited tool-use learning and was associated with increased gamma band activity …

Overcoming Barriers To Human Papillomavirus Vaccination In Guangdong Province, China, Shuaijing Zhang, Shiqi Li, Jingtai Ma, Guiyuan Ji, Zhifeng Li, Siyi Chen, Fenglin Zhang, Xingfen Yang, Jianpeng Xiao, Rong Cao, Chenggang Wu, Wei Wu

Faculty, Staff and Student Publications

Human papillomavirus (HPV) infection remains a critical public health challenge in China, particularly in Guangdong Province, where HPV-52, 16, and 58 genotypes predominate, and male infection rates exceed 40%. Despite the successful implementation of a government-funded school-based program that has achieved 88% HPV vaccine coverage among adolescent girls, several persistent barriers, including genotype mismatch (the free HPV vaccine covers < 50% of high-risk local strains), regional disparities (80% vs. 60% for first-dose coverage), and exclusion of males, thwart progress toward herd immunity. Financial sustainability risks pose an even more significant threat to the expansion of HPV vaccination programs, especially in Guangdong province where annual expenditures exceed CNY 200 million. This review delves into Guangdong's pioneering efforts and proposes practical solutions: accelerating domestic multivalent HPV vaccine development, adopting gender-neutral vaccination policies, and leveraging mobile clinics for remote populations. These strategies not only provide a roadmap for China but also serve as valuable insight for other LMICs striving to overcome HPV-related inequalities.

An Intranasally Administered Igm Protects Against Antigenically Distinct Subtypes Of Influenza A Viruses, Ashwin Kumar Ramesh, Jwala Priyadarsini Sivaccumar, Xiaohua Ye, Luona Yang, Hailong Guo, Chen-Ni Chin, Sha Ha, John W Shiver, William R Strohl, Yan Xu, Haijuan Du, Tongqing Zhou, Ningyan Zhang, Kai Xu, Xinli Liu, Tong-Ming Fu, Zhiqiang An

Faculty, Staff and Student Publications

Engineering broadly neutralizing monoclonal antibodies (mAbs) targeting the hemagglutinin (HA) of Influenza A virus (IAV) is a promising approach for intervention of seasonal flu. However, HA plasticity often leads to resistant strains that compromise mAb potency as bivalent IgGs. Here we hypothesize that multimerization of anti-IAV antibodies as IgMs can enhance coverage and neutralization potency. Here, we construct 18 IgM antibodies from known broadly neutralizing IgGs targeting different IAV HA epitopes and evaluate their breadth and potency of neutralization against distinct H1N1 and H3N2 IAVs. The IgM version of receptor binding site-specific IgG F045-092 shows increased breadth and antiviral potency …

Enhanced And Interpretable Prediction Of Multiple Cancer Types Using A Stacking Ensemble Approach With Shap Analysis, Shahid Mohammad Ganie, Pijush Kanti Dutta Pramanik, Zhongming Zhao

Faculty, Staff and Student Publications

Background: Cancer is a leading cause of death worldwide, and its early detection is crucial for improving patient outcomes. This study aimed to develop and evaluate ensemble learning models, specifically stacking, for the accurate prediction of lung, breast, and cervical cancers using lifestyle and clinical data.

Methods: 12 base learners were trained on datasets for lung, breast, and cervical cancer. Stacking ensemble models were then developed using these base learners. The models were evaluated for accuracy, precision, recall, F1-score, AUC-ROC, MCC, and kappa. An explainable AI technique, SHAP, was used to interpret model predictions.

Results: The stacking …

Distinct Oxytocin Signaling Pathways Synergistically Mediate Rescue-Like Behavior In Mice, Feng-Rui Zhang, Juan Liu, Jieqi Wen, Zi-Yan Zhang, Yijia Li, Eric Song, Li Hu, Zhou-Feng Chen

2020-Current year OA Pubs

Spontaneous rescue behavior enhances the well-being and survival of social animals, yet the neural mechanisms underlying the recognition and response to conspecifics in need remain unclear. Here, we report that observer mice experience distress when encountering anesthetized conspecifics, prompting spontaneous rescue-like behavior toward the unconscious mice. This behavior facilitates the earlier awakening of anesthetized mice while simultaneously alleviating stress in the helper mice. Our findings reveal that endogenous oxytocin (OXT) release from the hypothalamic paraventricular nucleus (PVN) to the oxytocin receptor (OXTR) in the central nucleus of the amygdala (CeA) regulates the emotional component of rescue-like behavior. In contrast, OXT …

A Secretome Atlas Of Cardiac Fibroblasts From Healthy And Infarcted Mouse Hearts, Jasmin Bahr, Junedh M Amrute, Kory J Lavine, Et Al.

2020-Current year OA Pubs

Cardiac fibroblasts (CF) are key players after myocardial infarction (MI), but their signaling is only incompletely understood. Here we report a first secretome atlas of CF in control (cCF) and post-MI mouse hearts (miCF), combining a rapid cell isolation technique with SILAC and click chemistry. In CF, numerous paracrine factors involved in immune homeostasis are identified. Comparing secretome, transcriptome (SLAMseq), and cellular proteome disclose protein turnover. In miCF at day 5 post-MI, significantly upregulated proteins include SLIT2, FN1, and CRLF1 in mouse and human samples. Comparing the miCF secretome at days 3 and 5 post-MI reveals the dynamic nature of …

Pediatric Liver And Kidney Transplant Recipients Demonstrate Greater Serological Response To Sars-Cov-2 Vaccination Than Adults, Tobias Laue, Maria Pilar Ballester, Lily Meoli, Carl Grabitz, Eva Uson, Lorenzo Dantiga, Valerie Mclin, Montserrat Pujadas, Ângela Carvalho-Gomes, Ivan Sahuco, Ariadna Bono, Federico D'Amico, Raffaela Viganò, Elena Diago, Beatriz Tormo Lanseros, Elvira Inglese, Dani Martinez Vazquez, Annelotte Broekhoven, Marjolein Kikkert, Shessy P.Torres Morales, Sebenzile K. Myeni, Mar Riveiro-Barciela, Adriana Palom, Nicola Zeni, Alessandra Brocca, Annarosa Cussigh, Sara Cmet, Maria Desamparados Escudero-García, Matteo Stocco, Matteo Stocco

Peninsula Medical School

Background. Adult solid organ transplant recipients (SOTRs) have decreased responsiveness to severe acute respiratory syndrome coronavirus type 2 (SARS-CoV-2) vaccination and higher incidence of infection, but there are few data on the serological response in pediatric SOTR. The aim of this study was to determine serological response to SARS-CoV-2 vaccination in pediatric liver (LT) and kidney transplant (KT) recipients and compare it with adult SOTR. Methods. A European, prospective, multicenter study was performed. Samples were taken at 7 and 32 wk following COVID-19 vaccination and serological endpoints were measured by ELISA. Results. A total of 42 pediatric (16 post-LT and …

Bbnj Agreement And The Negotiation Process: An Analysis From Developing Country Perspective With Special Focus On Indonesia, Simi K K, Kavitha Chalakkal

Indonesian Journal of International Law

The governance of marine genetic resources from areas beyond national jurisdiction (ABNJ)and regulating access to this regime in all available and existing forms is a core issue before international policymakers. The recently concluded BBNJ Agreement negotiations for the conservation and sustainable use of marine biological resources from areas beyond national jurisdiction was a platform to negotiate for a new legal instrument to address the core issues related to marine diversity from ABNJ, as agreed under the UNGA Resolution 72/249 to address the problems identified in the 2011 package. Defining MGRs and developing appropriate access and benefit-sharing mechanisms for marine biological …