Digital Commons Network^™

Regulation Of ΣB Levels And Activity In Bacillus Subtilis, Andrew K. Benson, William G. Haldenwang

Department of Food Science and Technology: Faculty Publications

The sigB operon of Bacilus subtilis encodes (σ^B plus three additional proteins (RsbV, RsbW, and RsbX) that regulate σ^B activity. Using an anti-σ^B monoclonal antibody to monitor the levels of (σ^B protein, P_SPAC to control the expression of the sigB operon, and a ctc-lacZ reporter system to monitor σ^B activity, we observed that the rsbV and rsbW products control (σ^B activity at the ctc promoter independently of their effects on (σ^B levels. In contrast, RsbX was found to have no effect on expression of ctc when the sigB operon was controlled by …

Bacillus Subtilis ΣB Is Regulated By A Binding Protein (Rsbw) That Blocks Its Association With Core Rna Polymerase, Andrew K. Benson, William G. Haldenwang

Department of Food Science and Technology: Faculty Publications

σ^B is a secondary σ factor of Bacillus subtilis. RNA polymerase containing cr transcribes a subset of genes that are expressed after heat shock or the onset of the stationary phase of growth. Three genes (rsbV, rsbW, and rsbX), cotranscribed with the σ^B structural gene (sigB), regulate o dependent gene expression. RsbW is the primary inhibitor of this system with the other gene products acting upstream of RsbW in the σ^B regulatory pathway. Evidence is now presented that RsbW inhibits σ^B-dependent transcription by binding to crB and blocking …

Sex, Lies, And Goffman: Embodiment And Fabrication In The Age Of Aids, Michael R. Hill

Department of Sociology: Faculty Publications

The frequently inexorable fatality, pansexual communicability, and lengthy period of asymptomatic latency of HIV combine to form biological realities and ecological dangers that are individually and socially problematic at very deep levels. How can we think sociologically about the AIDS epidemic? Talcott Parsons’ concept of “sick role,”2 a venerable and productive staple of medical sociology, gives us little to work with here, precisely as sexually active, HIV-positive but undiagnosed and asymptomatic persons do not see themselves as “sick.” Nor, importantly, do others, including: sexual intimates, friends, family members, employers, and health professionals. The interpersonal face of HIV is often trusted, …

Time-Stratigraphic Reconstruction And Integration Of Paleopedologic, Sedimentologic, And Biotic Events (Willwood Formation, Lower Eocene, Northwest Wyoming, U.S.A.), Thomas M. Bown, Mary J. Kraus

United States Geological Survey: Staff Publications

Relative paleosol maturities are inversely proportional to the accumulation rates of the sediment upon which they formed, and are therefore excellent relative indicators of how much geologic time elapsed between any two horizons. An empirically-based model is advanced using paleosol maturities to estimate the relative geologic time separating any stratigraphic levels within the lower Eocene Willwood Formation. The revised Willwood time stratigraphy from this analysis helps evaluate the nature, tempo, and possible causes of three major episodes of mammalian appearance and disappearance. These faunal events are directly correlated with certain aspects of paleosol evolution in the Willwood Formation. That evolution …

Three New Species Of Nematodes Associated With Endemic Grape (Vitis) In California, Luma Al-Banna, Scott Lyell Gardner

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Three new species of nematodes were encountered during a study of natural diversity of nematode associates of native species of Vitis L. in California. Achromadora walkeri sp. n. was found in rhizosoil of the native California grape Vitis californica Bentham and is characterized by the position of the amphid (within the vicinity of both dorsal and ventral teeth), a relatively long stoma, and the absence of a prerectum. The other 2 species were plant parasitic criconematids: Criconemoides featherensis sp. n., found in association with roots of V. californica, is characterized by possessing strongly retrose annuli posterior to the vulva, …

The ΣB-Dependent Promoter Of The Bacillus Subtilis Sigb Operon Is Induced By Heat Shock, Andrew K. Benson, W. G. Haldenwang

Department of Food Science and Technology: Faculty Publications

σ^B, a secondary sigma factor of Bacillus subtilis, was found to increase 5- to 10-fold when cultures were shifted from 37 to 48°C. Western blot (immunoblot) analyses, in which monoclonal antibodies specific for the sigB operon products RsbV, RsbW, and σ^B were used to probe extracts from wild-type and mutant B. subtilis strains, revealed that all three proteins increased coordinately after heat shock and that this increase was dependent on σ^B but not RsbV, a positive regulator normally essential for σ^B-dependent sigB expression. Nuclease protection experiments of RNA synthesized after heat shock supported …

Chemical Tracing Techniques For Evaluating Rill Hydraulics, John E. Gilley, Eugene R. Kottwitz

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Development of water erοsiοn and surface water quality control practices requires information concerning the hydraulic characteristics of upland areas. The relatively small flow rates normally found within rills make measurement of hydraulic parameters difficult. Chemical tracing procedures, originally developed for stream and river systems, have been successfully used to measure rill flow properties. A chemical tracer of known concentration is added to the rill and by knowing the degree of dilutiοn at a downstream sampling point, flow rate can be calculated. Rill flow velocity can be measured by determining the time required for a slug of tracer material to travel …

Quail Iii: National Quail Symposium (Kansas City, Missouri, United States, July 14-17, 1992), Kevin E. Church, Thomas V. Dailey

Galliformes Specialist Group and Affiliated Societies: Conference Proceedings

General

The history of quail management with comments on pen-rearing, Edward L. Kozicky

Taxonomy and biogeography of New Norld quail, R. J. Gutierrez

Bobwhite and the "new" biology, John L. Roseberry

Quail methodology: Where are we and where do we need to be? Dean F. Stauffer

Attitudes of a select group of Illinois quail hunters, John L. Roseberry and W. D. Klimstra

Population biology

Population trends of quails in North America, Kevin E. Church, John R. Sauer, and Sam Droege

Potential polygamous breeding behavior in northern bobwhite, Paul D. Curtis, Brad S. Mueller, Phillip D. Doerr, Charles F. Robinette, and …

The Niobrara Valley Preserve: An Inventory Of A Biogeographical Crossroads, Gail E. Kantak, Steven P. Churchill

Transactions of the Nebraska Academy of Sciences and Affiliated Societies

This paper synthesizes results of a biological inventory of a 219-km² nature preserve in the central Niobrara Valley of Nebraska. The two-year inventory documented the presence of 80 species of mosses and liverworts, 581 of vascular plants, 130 of aquatic Invertebrates and 278 of vertebrate animals. Comparison with distribution records from four other counties in Nebraska shows that the Niobrara study site has higher vertebrate species diversity despite its smaller area. Although the study area is in the central grasslands of North America, many of the species here are more typical of forest biomes far to the east, north …

The Status Of Nebraska Fishes In The Missouri River. 1. Paddlefish (Polyodontidae: Polyodon Spathula), Larry W. Hesse, Gerald E. Mestl

Transactions of the Nebraska Academy of Sciences and Affiliated Societies

The mean larval paddlefish density was 60 times higher in the upper unchannelized section of the Missouri River in Nebraska compared to the lower unchannelized section, and was three times higher than the channelized section's density. Within the upper unchannelized section, 96.2% of the larvae were collected in the discharge of two tributaries, which lie in the lower one-third of the reach. Survival from larval to young-of-the-year stage (June through August) was highest during 1991 due, in part, to the recent drought. Reduced fluctuation of river stages occurred as a result of reduced runoff, which minimized the need to reduce …

The Status Of Nebraska Fishes In The Missouri River. 2. Burbot (Gadidae: Lota Lota), Larry W. Hesse

Transactions of the Nebraska Academy of Sciences and Affiliated Societies

Burbot harvest represented 5.1% of the fish-harvest in the tailwater of Gavins Point Dam in July 1956, one year after the dam was closed. Harvest increased from 510 in 1956 to 4,280 in 1958, but represented only 2% of the 1958 harvest. By 1961 burbot were no longer routinely caught in the tailwater, and their density decreased in the remainder of Nebraska's portion of the Missouri River. At present they are very rare and should be listed as endangered in Nebraska.

Transactions Of The Nebraska Academy Of Sciences Table Of Contents: Volume Xx (1993)

Transactions of the Nebraska Academy of Sciences and Affiliated Societies

Editorial Contents

Nebraska Academy of Sciences Officers, Policy Committee ............ ii

Editorial Board ............ iv

Membership Objectives and Friends of the Academy ............ v

Nebraska Association of Teachers and Science ............ vi

Preparation of manuscripts ............ inside back cover

Papers

The Niobrara Valley Preserve: And inventory of a biogeographical crossroads (Gail E. Kantak and Steven P. Churchill) ............ 1

The saline wetland-meadow vegetation and flora of the North Platte River Valley in the Nebraska panhandle (Steven B. Rolfsmeier) ............ 13

Seasonal trends in forage quality of plants in subirrigated meadows of the Nebraska Sandhills (Nichols, Duncan, and Clanton) ............ 25 …

Woody Vegetation Of A Disjunct Bur Oak (Quercus Macrocarpa) Forest In East-Central Nebraska, Debra Ann Beightol, Thomas B. Bragg

Transactions of the Nebraska Academy of Sciences and Affiliated Societies

Woody plant composition was assessed for three tree-size classes in two ravines of Oak Glen Wildlife Management Area, a disjunct oak forest in Seward County, Nebraska, using Importance Values (IV) obtained by the Point-Quarter method. Bur oak (Quercus macrocarpa Michx.) dominated the large-size class (>30 cm dbh) (IV = 258) but elms (Ulmus americana L. and U. rubra Muhl.) (IV = 130) dominated the forest in one ravine in the medium-size class (10-30cm dbh) and elm and hackberry (Celtis occidentalis L.) (IV = 114 and 27 respectively) dominated the small-size class (<10cm dbh). Bur oak in the small-size was low in abundance (IV = 9) and was absent from one of the study transects. These data on tree-size distribution in the extant forest suggests that the forest is likely to succeed from one presently dominated by bur oak, which appears to have characterized the presettlement forest, to one dominated primarily by elm or hackberry. Current management is likely to encourage a continuation of this succession.

Floristic Diversity In Ten Tallgrass Prairie Remnants Of Eastern Nebraska, Judith F. Boettcher, Thomas B. Bragg, David M. Sutherland

Transactions of the Nebraska Academy of Sciences and Affiliated Societies

Ten eastern Nebraska tallgrass prairie remnants, varying in size from one to 18 ha, were studied or visited during the growing seasons from 1979 to 1993. A total of 217 prairie plant species were recorded. Big bluestem (Andropogon gerardii), little bluestem (Andropogon scoparius), smooth brome (Bromus inermis subsp. inermis), New Jersey tea (Ceanothus herbaceus var. pubescens), false sunflower (Heliopsis helianthoides var. scabra), Kentucky bluegrass (Poa pratensis), and porcupine-grass (Stipa spartea) were prevalent in all the prairies although the specific floristic composition has been reported to vary …

Role Of Macroinvertebrates In Spring Diet And Habitat Use Of Sandhill Cranes, Craig A. Davis, Paul A. Vohs

Transactions of the Nebraska Academy of Sciences and Affiliated Societies

In the springs of 1989 and 1990, we collected 15 lesser sandhill cranes from native grasslands along the Platte River. Macroinvertebrates constituted 89% of the diet, whereas plants constituted 11%. Scarab beetles were the predominant macroinvertebrate food. Other foods included earthworms, snail shells, sedge tubers, ground beetles, and crane fly larvae. Cranes exhibited differential use patterns on the study area; they extensively used areas composed of wet meadow and lowland grassland, but minimally used areas composed entirely of lowland grassland. Macroinvertebrate numbers and biomass were significantly different between areas extensively used by cranes and areas minimally used. Recommendations for management …

The Saline Wetland-Meadow Vegetation And Flora Of The North Platte River Valley In The Nebraska Panhandle, Steven B. Rolfsmeier

Transactions of the Nebraska Academy of Sciences and Affiliated Societies

The saline wetlands associated with the Platte River have received very little attention from botanists since the 1890s, and several plants widespread in these habitats, such as rayless salt-marsh aster (Aster brachyactis), annual goldenweed (Haplopappus annuus) and thelypody (Thelypodium integrifolium) have been considered rare in the State. A floristic inventory of sixteen tracts of saline lowlands near the North Platte River in Garden, Morrill, and Scotts Bluff counties reveals the presence of 231 species of vascular plants (169 native and 62 introduced), including three Eurasian halophytes (Althaea officinalis, Najas marina, Spergularia marina) …

Nebraska Summary: S299 Agco Allis 5660, Nebraska Tractor Test Lab

Nebraska Tractor Tests

ABOUT THE TEST REPORT AND USE OF THE DATA The test data contained in this report are a tabulation of the results of a series of tests. Due to the restricted format of these pages, only a limited amount of data and not all of the tractor specifications are included. The full OECD report contains usually about 30 pages of data and specifications. The test data were obtained for each tractor under similar conditions and therefore, provide a means of comparison of performance based on a limited set of reported data. EXPLANATION OF THE TEST PROCEDURES Purpose The purpose of …

Nebraska Summary: S298 Agco Allis 5650, Nebraska Tractor Test Lab

Nebraska Tractor Tests

ABOUT THE TEST REPORT AND USE OF THE DATA The test data contained in this report are a tabulation of the results of a series of tests. Due to the restricted format of these pages, only a limited amount of data and not all of the tractor specifications are included. The full OECD report contains usually about 30 pages of data and specifications. The test data were obtained for each tractor under similar conditions and therefore, provide a means of comparison of performance based on a limited set of reported data. EXPLANATION OF THE TEST PROCEDURES Purpose The purpose of …

Nebraska Summary: S171 Massey Ferguson 3075, Nebraska Tractor Test Lab

Nebraska Tractor Tests

ABOUT THE TEST REPORT AND USE OF THE DATA The test data contained in this report are a tabulation of the results of a series of tests. Due to the restricted format of these pages, only a limited amount of data and not all of the tractor specifications are included. The full OECD report contains usually about 30 pages of data and specifications. The test data were obtained for each tractor under similar conditions and therefore, provide a means of comparison of performance based on a limited set of reported data. EXPLANATION OF THE TEST PROCEDURES Purpose The purpose of …

Nebraska Summary: S145 Case-Ih 5250, Nebraska Tractor Test Lab

Nebraska Tractor Tests

ABOUT THE TEST REPORT AND USE OF THE DATA The test data contained in this report are a tabulation of the results of a series of tests. Due to the restricted format of these pages, only a limited amount of data and not all of the tractor specifications are included. The full OECD report contains usually about 30 pages of data and specifications. The test data were obtained for each tractor under similar conditions and therefore, provide a means of comparison of performance based on a limited set of reported data. EXPLANATION OF THE TEST PROCEDURES Purpose The purpose of …

Nebraska Summary: S144 Case-Ih 5240, Nebraska Tractor Test Lab

Nebraska Tractor Tests

ABOUT THE TEST REPORT AND USE OF THE DATA The test data contained in this report are a tabulation of the results of a series of tests. Due to the restricted format of these pages, only a limited amount of data and not all of the tractor specifications are included. The full OECD report contains usually about 30 pages of data and specifications. The test data were obtained for each tractor under similar conditions and therefore, provide a means of comparison of performance based on a limited set of reported data. EXPLANATION OF THE TEST PROCEDURES Purpose The purpose of …

Nebraska Summary: S142 Case-Ih 5220, Nebraska Tractor Test Lab

Nebraska Tractor Tests

ABOUT THE TEST REPORT AND USE OF THE DATA The test data contained in this report are a tabulation of the results of a series of tests. Due to the restricted format of these pages, only a limited amount of data and not all of the tractor specifications are included. The full OECD report contains usually about 30 pages of data and specifications. The test data were obtained for each tractor under similar conditions and therefore, provide a means of comparison of performance based on a limited set of reported data. EXPLANATION OF THE TEST PROCEDURES Purpose The purpose of …

Nebraska Summary: S143 Case-Ih 5230, Nebraska Tractor Test Lab

Nebraska Tractor Tests

ABOUT THE TEST REPORT AND USE OF THE DATA The test data contained in this report are a tabulation of the results of a series of tests. Due to the restricted format of these pages, only a limited amount of data and not all of the tractor specifications are included. The full OECD report contains usually about 30 pages of data and specifications. The test data were obtained for each tractor under similar conditions and therefore, provide a means of comparison of performance based on a limited set of reported data. EXPLANATION OF THE TEST PROCEDURES Purpose The purpose of …

High-Level Expression Of A Heterologous Protein In The Milk Of Transgenic Swine Using The Cdna Encoding Human Protein C, William H. Velander, John L. Johnson, Raymond L. Page, Christopher G. Russell, Anuradha Subramanian, Tracy D. Wilkins, Francis C. Gwazdauskas, Christoph Pittius, William N. Drohan

William H. Velander Publications

Transgenic pigs were generated that produced human protein C in their milk at up to 1 g/liter. The gene construct was a fusion gene consisting of the cDNA for human protein C inserted into the first exon of the mouse whey acidic protein gene. These results demonstrate that the mouse whey acidic protein gene contains regulatory elements that can direct cDNA expression at high levels in the pig mammary gland. Recombinant human protein C that was produced at about 380 pg/ml per hr in transgenic pig milk possessed anticoagulant activity that was equivalent to that of protein C derived from …

Water Current, Volume 24, December 1992

Water Current Newsletter

Seminar Series to Feature Global Perspective
Report from the Director
Argentina, Brazil Toured by Group from Nebraska
Czech Water Specialists Enumerate Contamination Problems
UNL Environmental Specialists Impressed by Hungarians
Foreign Influences on Nebraska Water Law
Trade, Environment, and Development, Tied Together in U.S. Trade Policy
Slovakian Grad Student Studies Aquaculture Here; Wants to Establish Fish Farm in Czechoslovakia
EARTH with Nebraska Connections Trains Agriculturists
La Mancha Plateau Aquifer Stressed by Intensive Irrigation
Hungarian Research Says Their Nitrogen Fertilizer Use Down
What's New?
Irrigation Association Centennial Spawns State Water-History Chronicle
Director of International Drought Information Center Receives Award
Dates to Use

Inventory Of Rare Plant Species In The Pine Ridge Area Of Nebraska (Dawes And Sioux County, Nebraska), Michael I. Fritz, Joyce Philips Hardy, Steven B. Rolfsmeier

Nebraska Game and Parks Commission: White Papers, Conference Presentations, and Manuscripts

The purpose of this project was to conduct a systematic inventory of rare plant species on the Pine Ridge District of the Nebraska National Forest. The objective of the inventory was to locate rare plant occurrences and compile information on their location, status and any possible threats. Four areas within the Pine Ridge District were identified as priority areas for the inventory fieldwork. The four tracts included the Soldier Creek Wilderness Area, a tract encompassing the East and West Ash Creek drainages, a middle unit tract, and a tract which straddles U.S. Highway 385 and encompasses a number of timber …

Ard News December 1992

Agricultural Research Division: News and Annual Reports

CONTENTS:

SEASONS GREETINGS
ORGANIZATIONS THAT SUPPORT AGRICULTURAL RESEARCH IN LAND GRANT UNIVERSITIES
ARDC INTEGRATED FARM
THE STATE AGRICULTURAL EXPERIMENT STATION SYSTEM
REPRESENTATION ON REGIONAL COMMITTEES
ARD ADMINISTERED GRANT AND AWARDS PROGRAMS
ARD ADVISORY COUNCIL UPDATE
PROPOSALS SUBMITTED FOR FEDERAL GRANTS
NEW OR REVISED PROJECTS
GRANTS & CONTRACTS RECEIVED OCTOBER & NOVEMBER, 1992

The Probe, Issue 128 - December 1992

The Probe: Newsletter of the National Animal Damage Control Association

Contents:

Reflections On An Onerous Deed
CALENDAR OF UPCOMING EVENTS
Coyote Urine Translocated by Flora
PETA Indictments Expected Soon
Plague Claims Bobcat Hunter
Colorado Voters Ban Spring Hunt; Arizonans Defeat Trapping Ban
Charter Member Says Good-Bye
Probe Should Be Advocate for ADC Activities
Bill Fitzwater III
Next Issue Jan/Feb
NADCA Renewals and Donations
New Logo Chosen for NADCA!

The Prairie Naturalist Volume 24, No.4 December 1992

The Prairie Naturalist

NEW VASCULAR PLANT RECORDS FOR NORTIl DAKOTA ▪ B. L. Heidel, A. J. Duxbury, W.T. Barker, and J. R. Challey

GROWTH OF BLUEGILLS AND YELLOW PERCH IN SOUTH DAKOTA WATERS ▪ D.w. Willis, J.P. Lott, C.S. Guy, and D.O. Lucchesi

SEASONAL VARIATION IN SAMPLING DATA FOR WALLEYE AND SAUGER COLLECTED WITH GILL NETS FROM LAKE SAKAKAWEA, NORTH DAKOTA ▪ S.W. Mero andD.W. Willis

OBSERVATIONS ON NESTING OF THE AMERICAN BITTERN IN NORTIlWEST MINNESOTA ▪ W D. Svedarsky

FOOD HABITS OF MOURNING DOVES IN EAST CENTRAL SOUTH DAKOTA ▪ J.T. Van't Hul and J.A. Jenks

DISTRIBUTION OF TIlE PYGMY SHREW IN …

Loss Of Infectivity By Progeny Virus From Alpha Interferon- Treated Human Immunodeficiency Virus Type 1-Infected T Cells Is Associated With Defective Assembly Of Envelope Gp120, Brian D. Hanson, Peter L. Nara, Radha K. Maheshwari, Girmel S. Sidhu, John G. Bernbaum, David Hoekzema, Monte S. Meltzer, Howard Gendelman

Nebraska Center for Virology: Faculty Publications

Levels of human immunodeficiency virus (HIV) DNA, RNA, or p24 antigen and reverse transcriptase activity in T-cell cultures treated with 500 IU of recombinant alpha interferon (rIFNα) per ml were comparable to those in control cultures. Radioimmunoprecipitation analysis of proteins in lysates of IFN-treated T cells documented a marked accumulation of HlV proteins. Localization of gp120 by immunofluorescence showed a diffuse pattern in IFN-treated cells quite distinct from the ring pattern in untreated control cells. That large quantities of a120 in aberrant cell compartments might affect HlV morphogenesis was confirmed in infectivity studies: virions from IFN-treated cells were 100- to …