Digital Commons Network^™

Isolation And Characterization Of New Wild-Type Isolates Of Bovine Lentivirus, David Suarez, Martin Van Der Maaten, Charles Wood, Ceclia Whetstone

Nebraska Center for Virology: Faculty Publications

Two new isolates of bovine lentivirus, also known as bovine immunodeficiency-like virus (BIV), were obtained from a seropositive cattle herd in Florida. This is the first report of new isolates of BIV since the original BIV strain, R29, was isolated in 1%9. The two new BIV isolates were derived from blood butFy coat cells cocultivated in vitro with fetal bovine lung cell cultures. The new isolates differed in vitro from the original R29 isolate in replication and syncytium formation in fetal bovine lung cells. Both new isolates were confirmed as BIV by immunofluorescence assay, Western blotting (immunoblotting), and polymerase chain …

Inheritance Of Freezing Resistance In Tuber-Bearing Solanum Species: Evidence For Independent Genetic Control Of Nonacclimated Freezing Tolerance And Cold Acclimation Capacity, Julie M. Stone, Jiwan P. Palta, John B. Bamberg, Laurie S. Weiss, James F. Harbage

Department of Biochemistry: Faculty Publications

Frost or winter survival is regarded as a complex trait with polygenic inheritance. Two maijor components of this survival in crop plants are freezing tolerance in the nonacclimated state and cold acclimation capacity. To date researchers have not distinguished the two components as separate heritable traits. The mode of inheritance of these two traits was investigated in F1 and backcross populations of two wild diploid potato species (Solanum commersonii and Solanum cardiophyllum) exhibiting extremes of freezing tolerance and acclimation capacity. Precise assessment of these two traits allowed distinction of small but significant differences among genotypes. The two traits …

Nubenocephalus Nebraskensis N. Gen., N. Sp. (Apicomplexa: Actinocephalidae) From Adults Of Argia Bipunctulata (Odonata: Zygoptera), Richard E. Clopton, Tamara J. Percival Cook, John J. Janovy Jr.

John J. Janovy, Jr. Publications

Nubenocephalus nebraskensis n. gen., n. sp. (Apicomplexa: Actinocephalidae) is described from trophozoites, sporonts, gamonts, and oocysts collected from adult Argia bipunctulata (Odonata: Zygoptera). The new genus is distinguished from existing acanthosporine genera by elongate dodecahedral oocysts, without equatorial faces, that are hexagonal in equatorial cross section with equatorial and terminal spines. The epimerite is very broadly ovoid and truncated posteriorly with equatorial lobiform tumidi that are posteriorly bilobate and do not form hooks, spines, or digitiform processes. The epimerite is borne on a long, slender stalk. The new species is also reported from naiads ofArgia bipunctulata and adults and naiads …

Nonenzymatic Glycosylation Of Lepidopteran-Active Bacillus Thuringiensis Protein Crystals, Meenakshi Bhattacharyi, Bradley A. Plantz, Jane D. Swanson-Kobler, Kenneth W. Nickerson

Papers in Microbiology

We used high-pH anion-exchange chromatography with pulsed amperometric detection to quantify the monosaccharides covalently attached to Bacillus thuringiensis HD-1 (Dipel) crystals. The crystals contained 0.54% sugars, including, in decreasing order of prevalence, glucose, fucose, arabinose/rhamnose, galactose, galactosamine, glucosamine, xylose, and mannose. Three lines of evidence indicated that these sugars arose from nonenzymatic glycosylation: (i) the sugars could not be removed by N- or O-glycanases; (ii) the sugars attached were influenced both by the medium in which the bacteria had been grown and by the time at which the crystals were harvested; and (iii) the chemical identity and stoichiometry of the …

Cellular Track Model For Study Of Heavy Ion Beams, Judy L. Shinn, Robert Katz, Francis A. Cucinotta, John W. Wilson, Duc M. Ngo

Robert Katz Publications

Track theory is combined with a realistic model of a heavy ion beam to study the effects of nuclear fragmentation on cell survival and biological effectiveness . The effects of secondary reaction products are studied as a function of depth in a water column. Good agreement is found with experimental results for the survival of human T-1 cells exposed to monoenergetic carbon, neon, and argon beams under aerobic and hypoxic conditions. The present calculation, which includes the effect of target fragmentation, is a significant improvement over an earlier calculation because of the use of a vastly improved beam model with …

Extending The Symbiotype Concept To Host Voucher Specimens, Daniel R. Brooks

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Recently, Frey, Yates, Duszynski, Gannon, and Gardner (1992) suggested that parasitologists deposit specimens of hosts from which type specimens of new parasite species are collected. Their proposal was directed especially at systematic and evolutionary biologists. Frey et al. (1992) pointed out that, whether one emphasizes microevolutionary (e.g., Price, 1980) or macroevolutionary (e.g., Brooks and Mc- Lennan, 1993) aspects of parasite evolutionary biology, much of the context of evolution involves hosts. Robust estimates of the selective effects and the patterns of diversification of host associations require the best possible estimates of the host species involved. This is …

Hexapod Herald - Vol. 4, No. 4, July 1, 1993

Hexapod Herald and Other Entomology Department Newsletters

Publications

Grants

Thank you

Congratulations

Welcome

Calendar of events

Travelers

Miscellaneous

Happy birthday

Nebline, July 1993

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

On-the-Grow: Horticulture News

Ag Update: Agricultural News & Events

Food, Families, and Finance

The Cloverline: 4-H News & Events Extension Calendar

Expanded Food and Nutrition Education Program (EFNEP)

Home Extension Club News

and other extension news and events

The Probe, Issue 134 - July 1993

The Probe: Newsletter of the National Animal Damage Control Association

CONTENTS:
Bats, bats, bats...Dealing With Unwanted Guests
CALENDAR OF UPCOMING EVENTS
ADC Shares Wildlife Society's Group Achievement Award
Senate Bill Bans Hunters From California Desert
Vultures Pull the Plug in Tennessee
Bison Brucellosis Research Halted
Oregon Refinery Plans to "Scare" Birds
Nominations Open for 1993-94 NADCA Officers
New Pest Control Handbook Out

Wildlife Health Centre Newsletter, Volume 1-3, Summer 1993

Canadian Cooperative Wildlife Health Centre: Newsletters and Publications

Work Underway at CCWHC
Residents in Wildlife Pathology
Rabies in Canadian Wildlife
Drowning of herring gull chicks
Antifreeze poisoning in a raccoon
Egg binding in a cormorant and a northern gannet
Loon mortality
Unhatched piping plover eggs
Salmonellosis in ring-billed gull
Lead poisoning of swans
Loon Mortalities
Pesticide poisoning of birds
Congenital defects in piscivorous birds
Yersiniosis in beavers
Canine distemper
Botulism
Mycotoxins in blighted grain: a risk to waterfowl?
Anthrax in bison in the Northwest Territories
Duck plague in British Columbia
Strychnine poisoning in gulls
Tree swallow nestling mortality
Phorate poisoning in northern harriers

Center For Sustainable Agricultural Systems Newsletter, July/August 1993

Center for Sustainable Agricultural Systems: Newsletter (1993–2000)

Contents:

Proposed North Central Institute for Agroecology

Call for LISA/ACE Preproposals

New Agency Formed in Interior Department

National Sustainable Ag Advisory Council Visits Nebraska

President's Council on Sustainable Development

Earthbound Project at Nebraska State Fair

Midwest Flooding Increases Soil Erosion

Report Calls for Action on Pesticides

Status of Federal Programs

Effects Of Offshore Oil And Gas Development: A Quarterly Current Awareness Bibliography, July 1993, Sue Ann Gardner

University of Nebraska-Lincoln Libraries: Faculty Publications

The July 1993 issue of Effects of Offshore Oil and Gas Development: A Quarterly Current Awareness Bibliography.

Agricultural Research Division 107th Annual Report 1993

Agricultural Research Division: News and Annual Reports

It is a pleasure to provide the 107th Annual Report of the UNL Agricultural Research Division (ARD). This report contains lists of current faculty; active research projects; refereed journal articles, books, and book chapters published; theses and dissertations written; germplasm/cultivars released; and awards received by faculty. Also included are brief descriptions of accomplishments from selected research projects and the financial report for the period July 1, 1992, to June 30,1993. This report was compiled in compliance with the intent of the law of the State of Nebraska that established the Nebraska Agricultural Experiment Station on March 31, 1887.

Foreword ... …

Estimation Of The Parameters Involved In A First-Order Autoregressive Process For Contemporary Groups, K. M. Wade, R. L. Quaas, L. Dale Van Vleck

Department of Animal Science: Faculty Publications

A methodology was developed for estimating the parameters involved in a first-order autoregressive process; these parameters comprise a variance component associated with the random effect, a correlation coefficient, p, and a residual variance. These parameters were estimated using REML with an expectation maximization algorithm. For two single trait analyses (milk and fat production being the dependent variable), the example chosen for the analyses was year-month -- treated as random and following a first-order autoregressive process within fixed herd. Initially, estimates failed to converge, possibly because of a time trend in the data, which was not accounted for by the model. …

The Prairie Naturalist Volume 25. No. 2. June 1993

The Prairie Naturalist

Paul B. Kannowski, Editor

Nikki R. Seabloom, Assistant Editor

Douglas H. Johnson, Book Review Editor

CONTENTS

RAPID GROWTH OF CHANNEL CATFISH IN FLAMING GORGE RESERVOIR, WYOMING ▪ C. B. Alexander and W. A. Hubert

MANAGEMENT EVALUATION OF BODY CONDITION AND POPULATION SIZE STRUCTURE FOR PADDLEFISH: A UNIQUE CASE ▪ M L. Brown and B. R. Murphy

DISTRIBUTION OF THE RIBBON LEECH IN NORTH DAKOTA ▪ C. M. Pennuto and M. G. Butler

SELENIUM IN EARED GREBE EMBRYOS FROM STEWART LAKE NATIONAL WILDLIFE REFUGE, NORTH DAKOTA ▪ M. M. Olson and D. Welsh

USING GEOGRAPHIC VARIATION TO PREDICT BREEDING LOCALES OF …

The Probe, Issue 133 - June 1993

The Probe: Newsletter of the National Animal Damage Control Association

CONTENTS:
ADC Eastern Region Highlights and Initiatives
Access to Information
Wildlife Hazards at Airports
CALENDAR OF UPCOMING EVENTS
Animal Rights Position Statement Issued by Minnesota
Hunters Should Check Local Requirements Before Planning Out-of-State Forays
New Live Trap Available
NADCA Nominating Committee Needs Members
Berry man Institute Dedicated
Get Your NADCA Hats Here!
Publications Available
Proceedings Available
Membership Votes Dues Increase
NRA Internships Available
Aquaculture Initiatives
Endangered Species Protection and Wildlife Enhancement

Nebline, June 1993

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

On-the-Grow: Horticulture News

Ag Update: Agricultural News & Events

Food, Families, and Finance

The Cloverline: 4-H News & Events

Extension Calendar

Expanded Food and Nutrition Education Program (EFNEP)

Home Extension Club News

and other extension news and events

Ard News June 1993

Agricultural Research Division: News and Annual Reports

CONTENTS:
COMMENTS FROM THE DEAN
ARD ADVISORY COUNCIL UPDATE
FY 1994 CSRS BUDGET
ROUTING RESEARCH PROPOSALS
IMPORTANCE OF PROJECT REVIEWS
INDIRECT COST RATES IN THE NORTH CENTRAL REGION
PROTECTING INTELLECTUAL PROPERTY
GRANTS AND CONTRACTS RECEIVED APRIL & MAY, 1993
FEDERAL REGISTER GRANT NOTICES
SECOND NATIONAL IPM SYMPOSIUM
PROPOSALS SUBMITTED FOR FEDERAL GRANTS
NEW OR REVISED PROJECTS
WHAT A DOLLAR SPENT FOR FOOD PAID FOR IN 1992

Two New Species Of Lestodiplosini (Diptera: Cecidomyiidae) Preying On Homoptera And Thysanoptera In Southern Florida, Raymond Gagne, Fred Bennett

USDA Systematic Entomology Laboratory

Two new species of predaceous Lestodiplosini from southern Florida are described and illustrated. Lestodiplosis laticaulis Gagne preys on Diaspis echinocacti (Bouche) (Homoptera: Coccoidea: Diaspididae), and Thripsobremia thripivora Gagne preys on Gynaikothrips ficorum (Marchal) (Thysanoptera: Phlaeothripidae) and Trioza magnoliae (Ashmead) (Homoptera: Psyllidae). The new species are compared to their congeners and the status of Thripsobremia is discussed.

Water Current, Volume 25, No. 1, June 1993

Water Current Newsletter

Water Resources Tour Set to See State's Sites in July
From the Director: Award of Two National Grants Reflects High Level of Activity
New Communications Associate Joins Staff at UNL Water Center
Coalition Issues Agenda
Michigan Video Illustrates Local Wellhead Program
Students Issue Verdict on Dam
Call for Papers
Danube Damming Project Controversial
Study on Sandhills Available
Soil and Water Sciences Tour Features Labs, Urban Water Sites
New Water Organization Formed
UNL Soil Scientist Conducts Pilot Study
FishAmerica Offers Grants
Book Tells History of Nebraska and Its Water
Festival to Celebrate State's Natural Resources

The Polymerase Chain Reaction And Plant Disease Diagnosis, Joan M. Henson, Roy C. French

Department of Plant Pathology: Faculty Publications

The polymerase chain reaction (PCR) provides a simple, ingenious method to exponentially amplify specific DNA sequences by in vitro DNA synthesis. Three essential steps to PCR (Figure 1) include (a) melting of the target (b) annealing of two oligonucleotide primers to the denatured DNA strands, and (c) primer extension by a thermostable DNA polymerase (123). Newly synthesized DNA strands serve as targets for subsequent DNA synthesis as the three steps are repeated up to 50 times. The specificity of the method derives from the synthetic oligonucleotide primers, which base-pair to and define each end of the target sequence to be …

Early Adolescent Family Formation, Lisa J. Crockett

Department of Psychology: Faculty Publications

In the United States, adolescent family formation has been considered a major social problem since the late 1970s. The sources of concern are multiple. First, teenage childbearing is associated with risks for the mother and her child, including health problems, reduced life chances, and a greater likelihood of living in poverty. Second, a large and increasing proportion of births to teenagers are nonmarital; this appears to compound these risks. Third, the rate of teenage childbearing in the United States far exceeds those in other Western, industrialized countries (Alan Guttmacher Institute, 1986). The high rate of adolescent pregnancy in the United …

Leptospira Genomes Are Modified At 5'-Gtac, David Ralph, Quideng Que, James L. Van Etten, Michael Mcclelland

Department of Plant Pathology: Faculty Publications

Genomic DNAs of 14 strains from seven species of the spirochete Leptospira were resistant to cleavage by the restriction endonuclease RsaI 1 (5'-GTAC). A modified base comigrating with m⁴C was detected by chromatography. Genomic DNAs from other spirochetes, Borrelia group VS461, and Serpulina strains were not resistant to h a 1 digestion. Modification at 5'-GTAm⁴C may occur in most or all strains of all species of Leptospira but not in all genera of spirochetes. Genus-wide DNA modification has rarely been observed in bacteria.

Immunocompromise In Gnotobiotic Pigs Induced By Verotoxin-Producing Escherichia Coli (O111:Nm), Jane Christopher-Hennings, Joann A. Willgohs, David H. Francis, Usha A. K. Raman, Rodney A. Moxley, David J. Hurley

School of Veterinary and Biomedical Sciences: Faculty Publications

A verotoxin-producing Escherichia coli serotype O111:NM strain (strain 10049; verotoxin 1 positive) persistently infected experimentally inoculated gnotobiotic pigs, causing attaching-effacing intestinal lesions and chronic diarrhea. Experiments were performed to determine whether persistent infection might be associated with immunocompromise of the host by this organism. Pigs inoculated with this strain had a significant reduction in peripheral blood lymphocytes and lower antibody titers to sheep erythrocytes compared with control pigs. Compared with pigs given a verotoxin-negative pathogenic strain of the same serotype (O111:NM, strain 2430), pigs inoculated with the verotoxin-positive strain had lower peripheral lymphocyte counts and proliferative responses to concanavalin A, …

Ecosystem Valuation: Combining Economics, Philosophy, And Ecology, F. Gregory Hayden

Department of Economics: Faculty Publications

Discussions regarding ecological crises often begin by casually identifying anthropocentric traits as responsible for the crises and by briefly explaining the need for holistic or macroscopic modeling. Yet, when modeling begins, policy scientists, philosophers, and social scientists usually are not included, and the models often are not ecological, but are rather narrowly biological or physical. The main purpose of this paper is to emphasize the need for broader and richer modeling and the need to recognize that environmental protection and enhancement is anthropocentric policymaking. The question is not whether it will be anthropocentric, but which anthropocentric values, beliefs, and philosophies …

Lagarocotyle Salamandrae N. Gen., N. Sp. (Monogenoidea, Polyonchoinea, Lagarocotylidae N. Ord.) From The Cloaca Of Rhyacotriton Cascadae Good And Wake (Caudata, Rhyacotritonidae) In Washington State, Delane C. Kritsky, Eric P. Hoberg, K. B. Aubry

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Lagarocotyle salamandrae n. gen., n. sp. (Lagarocotylidea n. ord., Lagarocotylidae n. fam.) is described from the cloaca of the Cascade torrent salamander, Rhyacotriton cascadae Good and Wake (Rhyacotritonidae), from the Lewis River and Wind River drainages of south-central Washington. Lagarocotyle n. gen. is characterized, in part, by a haptor armed with 16 hooks (14 submarginal, two subcentral), blind intestinal ceca, a large testis surrounding the germarium, an open male copulatory organ (not tubular), a dextroventral vaginal pore (at level of copulatory complex), and an egg lacking filaments; eyes, head organs, seminal receptacle, and haptoral anchors and bars are lacking. Lagarocotyle …

Dna Strand-Transfer Activity In Pea (Pisum Sativum L.) Chloroplasts, Heriberto D. Cerutti, A. T. Jagendorf

Center for Plant Science Innovation: Faculty Publications

The occurrence of DNA recombination in plastids of higher plants is well documented. However, little is known at the enzymic level. To begin dissecting the biochemical mechanism(s) involved we focused on a key step: strand transfer between homologous parental DNAs. We detected a RecA-like strand transfer activity in stromal extracts from pea (Pisum sativum L.) chloroplasts. Formation of joint molecules requires Mg2+, ATP, and homologous substrates. This activity is inhibited by excess single-stranded DNA (ssDNA), suggesting a necessary stoichiometric relation between enzyme and ssDNA. In a novel assay with Triton X-100-permeabilized chloroplasts, we also detected strand invasion of the …

Treatment Of Pea (Pisum Sativum L.) Protoplasts With Dna-Damaging Agents Induces A 39-Kilodalton Chloroplast Protein Immunologically Related To Escherichia Coli Reca, Heriberto D. Cerutti, H. Z. Ibrahim, A. T. Jagendorf

Center for Plant Science Innovation: Faculty Publications

Organisms must have efficient mechanisms of DNA repair and recombination to prevent alterations in their genetic information due to DNA damage. There is evidence for DNA repair and recombination in plastids of higher plants, although very little is known at the biochemical level. Many chloroplast proteins are of eubacterial ancestry, suggesting that the same could be true for the components of a DNA repair and recombination system. A 39-kD protein, immunologically related to Escherichia coli RecA, is present in chloroplasts of pea (Pisum sativum L.). Bandshift gel assays suggest that it binds single-stranded DNA. Its steady-state level is increased …

Rapid Assessment Of Drug Susceptibilities Of Mycobacterium Tuberculosis By Means Of Luciferase Reporter Phages, William R. Jacobs Jr., Raul G. Barletta, Rupa Udani, John Chan, Gary Kalkut, Gabriel Sosne, Tobias Kieser, Gary J. Sarkis, Graham F. Hatfull, Barry R. Bloom

School of Veterinary and Biomedical Sciences: Faculty Publications

Effective chemotherapy of tuberculosis requires rapid assessment of drug sensitivity because of the emergence of multidrug-resistant Mycobacterium tuberculosis. Drug susceptibility was assessed by a simple method based on the efficient production of photons by viable mycobacteria infected with specific reporter phages expressing the firefly luciferase gene. Light production was dependent on phage infection, expression of the luciferase gene, and the level of cellular adenosine triphosphate. Signals could be detected within minutes after infection of virulent M. tuberculosis with reporter phages. Culture of conventional strains with antituberculosis drugs, including isoniazid or rifampicin, resulted in extinction of light production. In contrast, …

Center For Sustainable Agricultural Systems Newsletter, May/June 1993

Center for Sustainable Agricultural Systems: Newsletter (1993–2000)

Contents:

Sustainable Ag Tour

ERS Sustainable Ag Initiative

Highlights from "Farm Bill" Seminar Series

Rural Policy Symposium Proceedings

SARE Grant Programs

SARE Funding - Chapters 2 & 3

Sustainable Agriculture Directory of Expertise

Integrated Farm Projects - ARDC

Study of Farm Family Health

Conference on Science and Sustainability