Digital Commons Network^™

An Overlooked Epidemic: Pediatric Oral Health Concerns On The Rise In Rural Communities, Emma Verbrugge

Honors Program: Senior Projects (Public)

Pediatric oral health is a concern for many dentists and parents as they lay the foundation for long-term proper hygiene in children. Early childhood caries (ECC) is a disease of chronic tooth decay marked as the most common chronic disease of childhood in the world. Children living in rural areas are even more susceptible to this disease and thus significant attention is needed to curve these numbers. The consequences of ECC are much more than just cosmetic, but include heightened risk of gingivitis, periodontal disease, diabetes, cardiovascular disease, and much more. A foundational cause of poor dental hygiene lies with …

Influence Of Fertilizer Rate On Swollen Stem Formation (“Bulbing”) And Vitamin C Content In Different Kohlrabi Cultivars, Mark Iradukunda, Paul E. Read

Honors Program: Senior Projects (Public)

Kohlrabi (Brassica oleracea Gongylodes Group) is a vegetable crop found in the Brassicaceae, and it is rich in antioxidants such as vitamin C. Vitamin C helps in oxidative damage repair and strengthens the immune system, so exploring vitamin C sources may contribute to improving human health. The objective of this study was to determine the effect of different fertilizer rates on kohlrabi yield, in terms of the swollen stem and the vitamin C content, and if those effects are similar in different kohlrabi cultivars. In this greenhouse study, we fertilized four potted kohlrabi cultivars with a common water-soluble greenhouse fertilizer …

A Glass Bead Semi‑Hydroponic System For Intact Maize Root Exudate Analysis And Phenotyping, Martha G. Lopez-Guerrero, Peng Wang, Felicia Phares4, Daniel P. Schachtman, Sophie Alvarez, Karin Van Dijk

Department of Biochemistry: Faculty Publications

Background: Although there have been numerous studies describing plant growth systems for root exudate collection, a common limitation is that these systems require disruption of the plant root system to facilitate exudate collection. Here, we present a newly designed semi-hydroponic system that uses glass beads as solid support to simulate soil impedance, which combined with drip irrigation, facilitates growth of healthy maize plants, collection and analysis of root exudates, and phenotyping of the roots with minimal growth disturbance or root damage.

Results: This system was used to collect root exudates from seven maize genotypes using water or 1 mM CaCl …

Disruption Of Claudin-Made Tight Junction Barriers By Clostridium Perfringens Enterotoxin: Insights From Structural Biology, Chinemerem P. Ogbu, Sourav Roy, Alex J. Vecchio

Department of Biochemistry: Faculty Publications

Claudins are a family of integral membrane proteins that enable epithelial cell/cell interactions by localizing to and driving the formation of tight junctions. Via claudin self-assembly within the membranes of adjoining cells, their extracellular domains interact, forming barriers to the paracellular transport of small molecules and ions. The bacterium Clostridium perfringens causes prevalent gastrointestinal disorders in mammals by employing an enterotoxin (CpE) that targets claudins. CpE binds to claudins at or near tight junctions in the gut and disrupts their barrier function, potentially by disabling their assembly or via cell signaling means—the mechanism(s) remain unclear. CpE ultimately destroys claudin-expressing cells …

Expression Of Atwri1 And Atdgat1 During Soybean Embryo Development Influences Oil And Carbohydrate Metabolism, Cintia Lucía Arias, Truyen Quach, Tu Huynh, Hanh Nguyen, Ademar Moretti, Yu Shi, Ming Guo, Amira Rasoul, Kyujung Van, Leah Mchale, Thomas E. Clemente, Ana Paula Alonso, Chi Zhang

Center for Plant Science Innovation: Faculty Publications

Soybean oil is one of the most consumed vegetable oils worldwide. Genetic improvement of its concentration in seeds has been historically pursued due to its direct association with its market value. Engineering attempts aiming to increase soybean seed oil presented different degrees of success that varied with the genetic design and the specific variety considered. Understanding the embryo’s responses to the genetic modifications introduced, is a critical step to successful approaches. In this work, the metabolic and transcriptional responses to AtWRI1 and AtDGAT1 expression in soybean seeds were evaluated. AtWRI1 is a master regulator of fatty acid (FA) biosynthesis, and …

Sesamum Indicum Diet Prevents Hyperlipidemia In Experimental Rats, Muinat M. Adeyanju, Idris A. Saheed, Oluwaseun I. Oyelekan, Taiwo A. Dele-Osibanjo, Ayodeji A. Adelegan, Adekunle J. Raimi, Samuel O. Olalekan, Olugbenga S. Alabi, Khadijat M. Alli

Department of Biochemistry: Faculty Publications

Cardiovascular diseases and metabolic complications caused by hyperlipidemia are the leading cause of death globally. In this study, the hypolipidemic potency of Sesamum indicum (SI) seeds was investigated. Of the thirty-five (35) male rats used in the study, five (5) were randomly selected for baseline measurements and thirty (30) were fed high fat diet (HFD) for four (4) weeks before random assignment into three (3) groups. The experimental group was treated with 50% SI seed, the positive control group was given a hypolipidemic drug, atorvastatin (5 mg/kg/day) while the untreated group served as the negative control. With SI administration, the …

No-Till Farming And Greenhouse Gas Fluxes: Insights From Literature And Experimental Data, S. J. Ruis, H. Blanco-Canqui, P. J. Jasa, V. L. Jin

Department of Agronomy and Horticulture: Faculty Publications

Tillage intensity may differently impact gaseous losses of C and N to the atmosphere, but data from long-term experiments are relatively few. Yet, this information is needed to better understand C and N losses and gains in agricultural systems. The objective of this study was to determine how tillage intensity affects soil greenhouse gas (GHG) fluxes (CO₂, N₂O, and CH₄) by comparing experimental data from moldboard plow (MP), chisel plow (CP), double disk (DD), and no-till (NT) soils after 38–40 yr of management in a rainfed corn (Zea mays L.)- soybean (Glycine …

Center For Grassland Studies, March 2022

Center for Grassland Studies: Newsletters

Contents

New Course: Research Experiences in Grasslands by Dan Uden

Center for Grassland Studies Policy Advisory Committee

Director’s Column by Jerry Volesky (Interim Director)

Educational Trunk to Focus on Grasslands by Cheryl Dunn

Form a Team and Grab Your Clubs by Scott Holly

Collaborative Adaptive Management for the Barta Brothers Ranch by Craig Allen

Nebraska Range Short Course, June 20-23

Registration Opens for 2022 Nebraska Ranch Practicum by Troy Walz

Nebraska Sandhills Rates as World’s Most Intact Prairie by Scott Schrage

Nebraska Youth Range Camp, June 6-9, Nebraska College of Technical Agriculture, Curtis, Nebraska

Timber Talk, Vol. 60, No. 1, March 2022

Timber Talk: Nebraska Forest Industry Newsletter

Lumber market reports

Hardwood lumber prices

Log matchmaking program launch, Heather Kingery

Save the date! – “Managing CarbonThrough Healthy Forests in theSouthern Great Plains”

Upcoming NFS cedar managementand utilization workshop series

Timber sales

Trading post

Untangling The Placentome Gene Network Of Beef Heifers In Early Gestation, Wellison J.S. Diniz, Lawrence P. Reynolds, Alison K. Ward, Pawel P. Borowicz, Kevin K. Sedivec, Kacie L. Mccarthy, Cierrah J. Kassetas, Friederike Baumgaertner, James D. Kirsch, Sheri T. Dorsam, Tammi L. Neville, J. Chris Forcherio, Ronald R. Scott, Joel S. Caton, Carl R. Dahlen

Department of Animal Science: Faculty Publications

The cotyledon and caruncle tissues provide a functional bridge between the fetus and the dam. However, the relationship between these tissues and the transcriptomic profile that underlies the tissue functions remains elusive. Herein we investigate the expression profile of cotyledon and caruncle from nulliparous beef heifers carrying female fetuses at day 83 of pregnancy to identify changes occurring across tissues that contribute to placental function and their tissue-specific roles. We identified 2654 differentially expressed genes [padj ≤ 0.05, abs(log2FC) ≥ 1], including nutrient transporters and paternally imprinted genes. We found key regulators of tissue function and differentiation, including FOXO4, GATA2, …

Annual Color Photo Section

Nebraska Bird Review

The next seven pages show examples of this year's theme: cavity nesting birds. A cavity nest can be in a birdhouse, a hole in a tree, a hole in a stream bank, a burrow in the ground, or even a mud nest the birds construct themselves.

Female Yellowbellied Sapsucker, photographer's residence, Garfield Co., 5 Jan 2022. Photo by Bob Gerten.

Louisiana Waterthrush with young at nest under bank overhang, Rock Creek Station State Historical Park, Jefferson Co., 18 May 2016. Photo by John Carlini.

Pygmy Nuthatch, GilbertBaker WMA, Sioux Co., 11 July 2021. Photo by Colleen Childers.

Winter Wren, Platte …

The Nebraska Bird Review Volume 90 Number 1, March 2022

Nebraska Bird Review

Winter Field Report, Dec. 2021 - Feb. 2022 by W. Ross Silcock

2021-2022 Christmas Bird Counts by Don Paseka

Annual Color Photo Section

Annual Treasurer's Report for 2021 by Jan Johnson

Subscription and Organization Information

2021-2022 Christmas Bird Counts, Don Paseka

Nebraska Bird Review

With the addition of the new Fort Niobrara circle, 19 counts were run during the 2021-2022 CBC season. Thirteen of them were run from December 14 through December 21 and the other six were done December 27 or later. The weather was mild to start the count period with almost all water open. As the count period progressed, the weather became more seasonable and much of the standing water froze, but conditions remained fairly benign with the exception of the Lake McConaughy count on January 2. Though it was cold but sunny on that day, the previous day’s snow and …

Winter Field Report, December 2021-February 2022, W. Ross Silcock

Nebraska Bird Review

After compiling and reviewing the observations reported to NEBIRDS, various Facebook groups, and eBird, I reiterate my leadoff comments from last winter: the increasing numbers of observers taking time to report turned up some fascinating trends. Those cited as well as those not cited, all contributed to the firmness of the database that allows better-supported conclusions to be drawn than from our past reliance all too often on anecdotal supposition. Continuing a trend pointed out last winter were the many records of species north and west of their expected winter ranges. As I suggested last winter, “it is tempting to …

Bacteriophage That Infect Gordonia Species Show Varying Host Specificity And Infection, Lindsay Birge

Honors Program: Senior Projects (Public)

Bacteriophage (phage) are an incredibly abundant species of virus that infect bacteria. One major characteristic of phage is their small host range- many phage are known to infect only one or a few hosts. This phage and host relationship has led to phage therapy recently becoming highlighted as a possible alternative to traditional antibiotics in light of the growing antibiotic resistance crisis. The study of phage host specificity has risen in line with this concept, as a phage with a more extensive host range can provide more opportunities for use as an antibiotic. Samples of phage previously isolated on a …

Impacts Of Temperature And Carbon Dioxide On Boro Rice Production, Sabiha Ferdous

Department of Agricultural and Biological Systems Engineering: Dissertations, Theses, and Student Research

There are different anticipated impacts of climate change (CC) on agriculture all over the world. Bangladesh as a developing country has potential vulnerability and negative impacts on CC. This change in meteorological parameters creates negative impacts on agricultural production which is a threat to food security and economic development. The country has recently achieved food security in rice which is mainly due to increased yields and area coverage of irrigated dry season (Boro) rice over the last decades. It is necessary to predict the future Boro rice production in accordance with CC as well as take supplementary actions to ensure …

Clash And Cooperation Of Ecofeminism And Postmodern Feminism: The Intersection Of Two Theories In Dystopic Literature, Ashton Koch

Honors Program: Senior Projects (Public)

Ecofeminism and postmodern feminism are regarded as opposite theories in their attempts to solve for ecological and patriarchal oppression. This thesis argues that these two theories are not mutually exclusive, and must work together to effectively combat oppression faced by the environment and by women. It uses two works of literature, Our Life in the Forest by Marie Darrieussecq and Oryx and Crake by Margaret Atwood to argue for the combination of ecofeminist and postmodern feminist strategies. Both works are dystopic in nature, portraying the destruction of the environment and patriarchal discourse against women. Ultimately, this thesis analyzes the strategies …

Biogeosciences Perspectives On Integrated, Coordinated, Open, Networked (Icon) Science, D. Dwivedi, A. L.D. Santos, M. A. Barnard, T. M. Crimmins, A. Malhotra, K. A. Rod, K. S. Aho, S. M. Bell, B. Bomfim, F. Q. Brearley, H. Cadillo-Quiroz, J. Chen, C. M. Gough, E. B. Graham, C. R. Hakkenberg, L. Haygood, G. Koren, E. A. Lilleskov, L. K. Meredith, S. Naeher, Z. L. Nickerson, O. Pourret, H. S. Song, M. Stahl, N. Taş, R. Vargas, S. Weintraub-Leff

Department of Agricultural and Biological Systems Engineering: Faculty Publications

This article is composed of three independent commentaries about the state of Integrated, Coordinated, Open, Networked (ICON) principles in the American Geophysical Union Biogeosciences section, and discussion on the opportunities and challenges of adopting them. Each commentary focuses on a different topic: (a) Global collaboration, technology transfer, and application (Section 2), (b) Community engagement, community science, education, and stakeholder involvement (Section 3), and (c) Field, experimental, remote sensing, and real-time data research and application (Section 4). We discuss needs and strategies for implementing ICON and outline short- and long-term goals. The inclusion of global data and international community engagement are …

Instructional Models For Course-Based Research Experience (Cre) Teaching, David I. Hanauer, Mark J. Graham, Rachel J. Arnold, Mary A. Ayuk, Mitchell F. Balish, Andrea R. Beyer, Kristen A. Butela, Christine A. Byrum, Catherine P. Chia, Hui Min Chung, Kari L. Clase, Stephanie Conant, Roy J. Coomans, Tom D'Elia, Jason Diaz, Arturo Diaz, Jean A. Doty, Nicholas P. Edgington, Dustin C. Edwards, Elvira Eivazova, Christine B. Emmons, Kayla M. Fast, Viknesh Sivanathan, And About 50 Others

School of Biological Sciences: Faculty Publications

The course-based research experience (CRE) with its documented educational benefits is increasingly being implemented in science, technology, engineering, and mathematics education. This article reports on a study that was done over a period of 3 years to explicate the instructional processes involved in teaching an undergraduate CRE. One hundred and two instructors from the established and large multi-institutional SEA-PHAGES program were surveyed for their understanding of the aims and practices of CRE teaching. This was followed by large-scale feedback sessions with the cohort of instructors at the annual SEA Faculty Meeting and subsequently with a small focus group of expert …

Assessing Pre-Literacy Behaviors In Infants And Toddlers: Psychometric Evaluation Of The Infant Toddler Literacy Assessment (Itla-3), Barbara J. Jackson, Christine Marvin, Amy J. Encinger, Nicole Buchholz, Becky Zessin

Department of Special Education and Communication Disorders: Faculty Publications

Assessment of emerging literacy in young children is generally limited to either skill development in children over 3 years of age or the quality and context of young children’s early literacy experiences. Although there has been promotion of their early literacy experiences, assessment of emerging pre-literacy behaviors in children younger than 3 years has yet to be organized into a single tool.

Preliminary work on the Infant Toddler Literacy Assessment (ITLA) has progressed through initial steps of scale development and shown promise as a criterionbased, standardized assessment for tracking children’s pre-literacy behaviors and guiding practitioners in supporting development of those …

Self-Compassion Mediates The Link Between Attachment Security And Intimate Relationship Quality For Couples Navigating Pregnancy, Tuyen Huynh, Eric Phillips, Rebecca L. Brock

Department of Psychology: Faculty Publications

Millions of couples navigate the transition from pregnancy to postpartum in a given year, and this period of change and adjustment in the family is associated with elevated risk for intimate relationship dysfunction. Self-compassion has the potential to promote skills that are essential for healthy adaptation (e.g., emotion regulation, greater openness and flexibility, more awareness of the needs of oneself and one’s partner). The overarching goal of the present study was to investigate the role of self-compassion in intimate relationship quality during pregnancy. A sample of 159 couples completed semi-structured interviews and questionnaires. Parents engaging in more compassionate self-responding during …

Furthering Women In Policing: How A Police Department's Duty Firearm Selection Process May Mitigate The Gender Disparity In Marksmanship, Jenna Prochnau

Honors Program: Senior Projects (Public)

Previous research has revealed that there are several benefits to increasing the representation of women in law enforcement, including less use of force, increased community trust, and better outcomes for domestic violence and sexual assault victims. While many police departments now recognize these benefits and are aiming to recruit more women, a problem persists because women are less likely to graduate from police academies than their male counterparts. A significant difference has been observed in the area of marksmanship, particularly, with more female recruits failing to meet firearm scoring standards. Currently, very little is known about how police departments select …

Uncovering The Potential Learning In Short-Term Study Abroad, Elizabeth Niehaus, Gudrun Nyunt

Department of Educational Administration: Faculty Publications

The purpose of this quantitative study was to identify the facets of intercultural learning that might be achievable through faculty-led, short-term study abroad. Exploring changes in various facets of students’ intercultural competence pre- to post-study abroad, we found that the largest gains were in the cultural knowledge domain. Findings also point to vast differences in how individual students do, and do not, show gains across multiple measures of intercultural competence after studying abroad.

Challenges In Gluten Analysis: A Comparison Of Four Commercial Sandwich Elisa Kits, Plaimein Amnuaycheewa, Lynn Niemann, Richard E. Goodman, Joseph L. Baumert, Steve L. Taylor

Department of Food Science and Technology: Faculty Publications

Gluten is composed of prolamin and glutelin proteins from several related grains. Because these proteins are not present in identical ratios in the various grains and because they have some differences in sequence, the ability to accurately quantify the overall amount of gluten in various food matrices to support gluten-free labeling is difficult. Four sandwich ELISAs (the R-Biopharm AG R5 RIDASCREEN®, the Neogen Veratox® R5, the Romer Labs AgraQuant® G12, and the Morinaga Wheat kits) were evaluated for their performance to quantify gluten concentrations in various foods and ingredients. The Morinaga and AgraQuant® G12 tests yielded results comparable to the …

Descripción De Una Nueva Especie De Tegmaleurodes Martin (Hemiptera: Aleyrodidae) De Guatemala, José Francisco García-Ochaeta

Insecta Mundi

Se describe Tegmaleurodes sanchezi, nueva especie (Hemiptera: Aleyrodidae), de Guatemala. Se proporcionan microfotografías de estructuras morfológicas del pupario, se comentan las características dife­renciales de la especie con sus congéneres y se incluye una clave para las especies.

Tegmaleurodes sanchezi, new species (Hemiptera: Aleyrodidae), is described from Guatemala. Photomicrographs of the morphological structures of the puparium are provided, distinguishing characteris­tics of the species from its congeners are discussed, and a key to the species is included.

El género de moscas blancas Tegmaleurodes fue descrito por Martin (2005), incluyendo las tres especies neotro­picales Tegmaleurodes lentus Martin, 2005, T. crustatus (Bondar, …

Taxonomic Changes Suggested By The Genomic Analysis Of Hesperiidae (Lepidoptera), Jing Zhang, Qian Cong, Jinhui Shen, Nick V. Grishin

Insecta Mundi

Our expanded efforts in genomic sequencing to cover additional skipper butterfly (Lepidoptera: Hesperiidae) species and populations, including primary type specimens, call for taxonomic changes to re­store monophyly and correct misidentifications by moving taxa between genera and proposing new names. Reconciliation between phenotypic characters and genomic trees suggests three new tribes, two new sub­tribes, 23 new genera, 17 new subgenera and 10 new species that are proposed here: Psolosini Grishin, new tribe (type genus Psolos Staudinger, 1889), Ismini Grishin, new tribe (type genus Isma Distant, 1886), Eetio­nini Grishin, new tribe (type genus Eetion de Nicéville, 1895), Orphina Grishin, new subtribe (type …

Enhancing The Bioaccessibility Of Lycopene From Tomato Processing Byproducts Via Supercritical Carbon Dioxide Extraction, Ali Ubeyitogullari, Ozan N. Ciftci

Department of Food Science and Technology: Faculty Publications

Tomato peel and seed from tomato processing industry are treated as waste; however, they contain lycopene, a high-value bioactive compound. In this study, lycopene was extracted from tomato peel and seed using supercritical carbon dioxide (SC–CO₂) and hexane, and the bioaccessibilities of lycopene in the SC-CO₂- and hexaneextracted oleoresins were investigated for the first time. The (Z)-lycopene content of the SC-CO₂-extracted oleoresin (69%) was higher than that of hexane-extracted oleoresin (45%). Separation of the insoluble fraction from the oleoresins increased the (Z)-lycopene contents of the SC-CO₂- and hexane-extracted oil fractions to 80% …

Synthesis And Evaluation Of 11c-Labeled Triazolones As Probes For Imaging Fatty Acid Synthase Expression By Positron Emission Tomography, James M. Kelly, Thomas M. Jeitner, Nicole N. Waterhouse, Wenchao Qu, Ethan J. Linstad, Banafshe Samani, Clarence Williams Jr., Anastasia Nikolopoulou, Alejandro Amor-Coarasa, Stephen G. Dimagno, John W. Babich

Department of Chemistry: Faculty Publications

Cancer cells require lipids to fulfill energetic, proliferative, and signaling requirements. Even though these cells can take up exogenous fatty acids, the majority exhibit a dependency on de novo fatty acid synthesis. Fatty acid synthase (FASN) is the rate-limiting enzyme in this process. Expression and activity of FASN is elevated in multiple cancers, where it correlates with disease progression and poor prognosis. These observations have sparked interest in developing methods of detecting FASN expression in vivo. One promising approach is the imaging of radiolabeled molecular probes targeting FASN by positron emission tomography (PET). However, although [¹¹C]acetate uptake by …

A Second Species Of The Genus Erebaces Pascoe, 1871 (Curculionidae: Molytinae: Cryptorhynchini) From The Philippines, Robert S. Anderson

Insecta Mundi

Erebaces woodruffi Anderson, new species (Curculionidae: Molytinae: Cryptorhynchini), from Palawan (Philippines) is described and illustrated. This is the second species of the genus Erebaces Pascoe described from the Philippines. It can be separated from Erebaces kidapawanus Pancini by the pair of divergent dorsal pale-scaled lines on the pronotum extended onto the elytra and by the form of the elytral tubercles.

The genus Erebaces Pascoe, 1871 was recently recorded in the Philippines based on the presence of E. kidapawanus Pancini, 2021 from Mindanao. The genus is easily recognized by the eyes finely faceted, protruding (toothed) elytral humeri, the deep rostral canal …

Revision Of The Checkered Beetle Genus Onychotillus Chapin (Coleoptera: Cleridae: Tillinae), Weston Opitz

Insecta Mundi

The West Indian genus Onychotillus Chapin (Coleoptera: Cleridae: Tillinae) is revised and includes O. androwi Opitz, new species, O. apiculus Opitz, new species, O. cinctipennis (Chevrolat,1874), O. cubana de Zayas,1988, O. dimidiatus de Zayas,1988, O. lineatus Opitz, new species, O. minutus de Zayas,1988, O. trinitatis de Zayas,1988, O. woodruffi Opitz, new species, and O. vittatus Chapin,1945.

Robert E. Woodruff was a consummate collector of insects, gems, and items of art. He was a superb taxonomist with a specialty in the beetle family Scarabaeidae. This contribution is dedicated …