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Relationships Of Nematodirus Species And Nematodirus Battus Isolates (Nematoda: Trichostrongyloidea) Based On Nuclear Ribosomal Dna Sequences, Steven A. Nadler, Eric P. Hoberg, Deborah S.S. Hudspeth, Lora G. Rickard

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Nuclear ribosomal sequence data from the internal transcribed spacers (ITS-1 and ITS-2), 5.8S subunit, and regions of the 18S and 28S genes were used to investigate sequence diversity among geographic samples of Nematodirus battus, and to infer phylogenetic relationships among Nematodirus species. Phylogenetic analysis of these data yielded strong support for relationships among species, depicting Nematodirus helvetianus and Nematodirus spathiger as sister-taxa and a clade of these two species and Nematodirus filicollis. This tree is consistent with caprine bovids as ancestral hosts, with a subsequent host shift to Bovinae in N. helvetianus. Eleven of fourteen N. battus sequences …

Population Trends In Furbearers In Nebraska, L. M. Landholt, Hugh H. Genoways

University of Nebraska State Museum: Mammalogy Papers

Population trends are documented from 1941 to 1997 for the 12 species of furbearing mammals harvested in Nebraska. Populations of red fox (Vulpes vulpes, raccoon (Procyon lotor), beaver (Castor canadensis), coyote (Canis lupus), and bobcat (Lynx rufus) have increased during this period. Populations of muskrat (Ondatra zibethicus), mink (Mustela vison), eastern spotted skunk (Spilogale putorius), long-tailed weasel (Mustela frenata), and striped skunk (Mephitis mephitis) have decreased. Populations of mink, eastern spotted skunk, long-tailed weasel, and striped skunk may have decreased …

Track Theory Predictions For Single-Hit Cell Survival, Robert Katz, Francis Cucinotta

Robert Katz Publications

The track theory of cell survival after heavy-ion irradiation is based on the probability of survival after a single-particle transit, called ‘‘ion-kill’’ (not track core), joined to the cumulative effect of δ rays from adjacent ions in a beam, called ‘‘gamma-kill’’ (not penumbra). The model offers a set of equations containing four parameters, E₀ and m for the single-hit multitarget statistical model for gamma-kill, and additionally σ₀ and κ for ion-kill. A single set of parameters is used to fit a family of survival curves obtained with ions of different LET simultaneously.

Expression Of A Dominant-Negative Mutant Of P21ras Inhibits Induction Of Nitric Oxide Synthase And Activation Of Nuclear Factor-Kb In Primary Astrocytes, Kalipada Pahan, Xiaojuan Lu, Michael J. Mckinney, Charles Wood, Faruk G. Sheikh, John R. Raymond

School of Biological Sciences: Faculty Publications

The present study underlines the importance of p21ras in regulating the inducible nitric oxide synthase (iNOS) in primary astrocytes. Bacterial lipopolysaccharides induced the GTP loading of p21ras, and the expression of a dominant-negative mutant of p21ras (Dp21ras) inhibited lipopolysaccharide-induced GTP loading in rat primary astrocytes. To delineate the role of p21ras in the induction of iNOS, we examined the effect of Dp21ras on the expression of iNOS and the production of nitric oxide. It is interesting that expression of Dp21ras markedly inhibited the production of nitric oxide and the expression of iNOS in lipopolysaccharide- and pro-inflammatory cytokine (tumor necrosis factor-a, …

Plant Quality And Spider Predation Affects Grasshoppers (Acrididae): Food-Quality-Dependent Compensatory Mortality, Mark A. Oedekoven, Anthony Joern

School of Biological Sciences: Faculty Publications

For insect herbivores, population responses reflect interactions among multiple factors, including abiotic conditions, food plant availability, and biotic interactions guided by food limitation and natural enemies. In this set of field experiments, we evaluated the interactive, dynamic effects of limited food supply and predation from wolf spiders (Lycosidae) on mortality in Ageneotettix deorum, a common grasshopper (Acrididae) from North American sandhills grasslands. We attempted to determine whether the combined effects of food availability and spider predation on mortality were (1) noncompensatory, with the effects of each factor summing to determine ultimate population size, or (2) compensatory, where the effect …

Biosystematics And Evolution Of The Triatominae = Biossistemática E Evolução De Triatomíneos, Christopher J. Schofield

Harold W. Manter Laboratory of Parasitology: Library Materials

Abstract

In this paper we summarize the systematics of the 130 currently recognized species of Triatominae and the key features of their evolutionary background. There is increasing evidence that the subfamily has polyphyletic origins, with the various tribes and species groups probably arising from different reduviid lineages in relatively recent times.

Resumo

Neste trabalho resume-se a sistemática das 130 espécies de triatomíneos atualmente reconhecidas, com os elementos principais de sua evolução. Existem evidências crescentes para a origem polifilética da subfamília. As diferentes tribos e grupos de espécies que a compõem teriam surgido recentemente, a partir de diferentes linhagens de reduviídeos.

Recognition Of Treponematoses In Post Repatriation X Ray And Cd Rom Nebraska Record, Karl J. Reinhard, Bruce Rothschild, Christine Rothschild, Larry Martin

Karl Reinhard Publications

Repatriation has compromised the opportunity to directly examine skeletons and to apply new diagnostic criteria and techniques. Pre-repatriation approaches to non-metric data acquisition, must make a number of assumptions: ( I ) Phenomena must be correctly identitied and segregated; (2) Criteria for severity must be specific to the phenomena studied; and (3) As criteria for disease rewgnition may change with time, it is valuable only as long as the raw data is also recorded. As part of data preservation, x-rays and CD-ROM images were recorded for skeletons trom Nebraska sites undergoing repatriation. This report concentrates on four of them 250K …

The Role Of Mummy Studies In Paleoparasitology, Adauto Araujo, Karl J. Reinhard, Luiz Fernando Ferreira

Karl Reinhard Publications

Paieoparasitology has advance during the past decade to the status of a statistically based science focused on problems of disease ecology and geographic distribution of parasitism. For most of its development, paleoparasitology has focused on the analysis of coprolites and latrine sediments. During the past few years, mummies have been increasingly included in paleoparasitology studies. We evaluate in this paper the interpretive value of mummies relative to other sources of paleoparasitological data.

EI estudio de la paleoparasitologia ha logrado transformarse, en esta ultima decada, en una ciencia basada en la estadistica, enfocada a temas tales como la ecologia de enfermedades …

Archaeoparasitology, Karl Reinhard

Karl Reinhard Publications

The field devoted to the identification of parasite remains in the archaeological record and the reconstruction of past human-parasite interactions. Parasitic disease has always been a major problem. Recent summaries of the prevalence of parasitic diseases in the world today show that there are 4.5 billion infections with all species of parasitic worm, 1 billion infections with giant intestinal roundworm (Ascaris lumbricoides), 750,000,000 infections with whipworms (Trichuris trichiura), 900,000,000 infections with hookworm (Ancylostoma duodenale and Necator americanus), 657,000,000 infections with filarial worms, 200,000,000 with blood flukes (schistosome species), and 489,000,000 infections with malaria. These infections cause between 1,590,000 to 3,130,000 …

Coprolite Analysis: The Analysis Of Ancient Human Feces For Dietary Data, Karl Reinhard

Karl Reinhard Publications

Although archaeological fieldwork is hot and dirty, the most “earthy” side of the discipline is the laboratory analysis of coprolites. Each coprolite contains the remains of one to several actual meals eaten in prehistory, and analysis of many coprolites provides a picture of ancient diet that is unique in accuracy.

The term coprolite originally referred to fossilized feces in paleontological context. In archaeology, the term broadened to refer to any formed fecal mass, including mineralized, desiccated, or frozen feces and even the intestinal contents of mummies. Coprolites contain the remains of animals (parasites) that lived in the humans, the foods …

Paleopharmacology, Karl Reinhard

Karl Reinhard Publications

An emerging field devoted to the exploration of the archaeological record for evidence of medicinal plants.

In the future, archaeology will have a role in pharmaceutical research. In the late twentieth century, much of the world’s pharmaceutical research has been based on ethnographic documentation of tribally recognized medicinal plants. Once the active chemical compounds in the plants are identified, they are either extracted or synthesized for commercial use. The American film Medicine Man depicts this approach. As shown in the film, such pharmacological research is threatened by declining tribal populations with loss of traditional herbal knowledge coupled with declining biodiversity. …

The Systematics Of The Genus Trichinella With A Key To Species, K. D. Murrell, J. Ralph Lichtenfels, Dante S. Zarlenga, E. Pozio

Harold W. Manter Laboratory of Parasitology: Faculty Publications

The authors review the major biological, biochemical, and molecular characters that are used to distinguish the seven Trichinella species (T. spiralis, T. nativa, T. britovi, T. pseudospiralis, T. murrelli, T. nelsoni, T. papuae) and three genotypes whose taxonomic status is yet uncertain (T-6, T-8, T-9). A comparison of host specificity, morphology, reproductive abilities, nurse cell development and freeze resistance is presented, along with useful biochemical and molecular markers. Finally, this information is used to construct a diagnostic key for the species. A phylogenetic classification of the species is needed.

Soil-Moisture Preferences And Soil-Use Behaviors Of Northern Pocket Gophers, Ray T. Sterner

United States Department of Agriculture Wildlife Services: Staff Publications

Factors affecting soil-contact and –manipulation behaviors of pocket gophers (Thomomys and Geomys spp.) are poorly understood. Delineation of these behaviors is crucial to development of new repellent systems that seek to exploit the fossorial activity of these rodents. In a laboratory study involving northern pocket gophers (Thomomys talpoides), I examined the effects(s) of gravimetric soil moisture (i.e., 0%, 5%, 10%, 15%, 20%, 25%) upon soil-contact and –use behaviors. Six gophers received successive, 0.5 h/day exposures to one of the moist soils compared to dry (0%) soil in a 2-choice apparatus. Times in each compartment and observed …

Strychnine Alkaloid And Avian Reproduction: Effects Occur At Lower Dietary Concentrations With Mallard Ducks Than With Bobwhite Quail, C. A. Pedersen, R. T. Sterner, M. J. Goodall

United States Department of Agriculture Wildlife Services: Staff Publications

Separate subchronic reproductive toxicity studies were conducted using mallard ducks (Anas platyrhynchos) and northern bobwhite quail (Colinus virginianus). Three groups (32/group; 16 male-female pairs) of 17-week-old ducks (F₀ generation) were fed Purina® Game Bird Breeder Layena® diets containing mean (±SD) 33.2 (±2.7), 68.9 (±1.8), and 140.9 (± 5.1) μg/g strychnine for 20 weeks, with some pairs in each group fed control diet during a subsequent 3-week recovery period. Three groups (32/group; 16 male-female pairs) of 19-week-old quail (F₀ generation) were fed similar diets containing mean (±SD) 279.2 (±10.1), 557.4 (±43.5), and 1,113.6 (±46.6) μg/g …

Integrated Pest Management Of Black Bear Reforestation Damage, Gary W. Witmer, Dale L. Nolte, William Stewart

United States Department of Agriculture Wildlife Services: Staff Publications

Black bear damage to commercial, coniferous trees on intensively managed public and private forest lands of the Pacific Northwest continues to be a problem for forest managers. Historically, methods such as relocation or spring hunts have been used in an effort to reduce bear density and damage. More recently, supplemental feeding has been used in an attempt to provide for the nutritional needs of bears during the damage period. Alternative silvicultural practices and repellents are being investigated for their ability to reduce the likelihood of bear damage. These and other methods need to be examined for their effectiveness, especially in …

Cormorant Research And Impacts To Southern Aquaculture, Scott Werner

United States Department of Agriculture Wildlife Services: Staff Publications

Several North American waterbird species were negatively affected by compromised environmental quality by the mid-twentieth century. Double-crested cormorant populations responded to increased environmental regulations in the United States in the early 1970s. The abundance of cormorants wintering in southern states (especially Alabama, Arkansas, Louisiana, and Mississippi) increased concurrently with a marked increase in catfish, crawfish, and bait fish production in these states since 1980, thus increasing regional concern regarding production losses to these industries. Cormorants wintering in Mississippi have increased nearly 225% since 1990. Food habit studies, bioenergetic predictions, and captive-bird foraging experiments indicate that individual cormorants consume approximately 0.5 …

Deer Population Management Through Hunting In A Suburban Nature Area In Eastern Nebraska, Kurt C. Vercauteren, Scott E. Hygnstrom

United States Department of Agriculture Wildlife Services: Staff Publications

The Fontenelle Forest Nature Area (FF) maintained a hands-off management policy for 30 years until it was recognized that white-tailed deer (Odocoileus virginianus) populations had grown to such levels that they were severely degrading native plant communities. In 1995, members of a community task force decided to sponsor annual nine-day hunting seasons on FF after learning that densities exceeded 28 deer/km². Archers harvested 85 antlerless deer in the FF upland areas adjacent to residential Bellevue, Nebraska during 1996 to 1998. Muzzleloader hunters removed 53 antlerless deer from the FF lowland areas. Archery and muzzleloader hunters harvested …

Evaluation Of A Radar-Activated, Demand-Performance Bird Hazing System, Gwen R. Stevens, Jamie Rogue, Richard Weber, Larry Clark

United States Department of Agriculture Wildlife Services: Staff Publications

We evaluated a radar-activated integrated hazing system for the protection of waterfowl at large contaminated ponds at a power plant. The hazing devices in the system included acoustic alarm calls, pyrotechnics and chemical repellents dispersed in the form of a bird tear-gas. Unlike, timed interval systems, or systems with random activation sequences, birds did not habituate to the demand-performance system tested. Over the course of a year, we documented that waterfowl were 12.5 times less likely to fly over the hazed contaminated ponds relative to a non-hazed control pond. Of the waterfowl that did fly over both ponds, the likelihood …

Urban-Suburban Prairie Dog Management: Opportunities And Challenges, Gary W. Witmer, Kurt C. Vercauteren, Karen Manci, Donna Dees

United States Department of Agriculture Wildlife Services: Staff Publications

The historic range of the black-tailed prairie dog has undergone dramatic declines in the last century, prompting concern about the species' long-term viability. While considered a pest by many, others believe that the species is a "keystone" element of prairie ecosystems. Urban-suburban land managers are challenged with preserving colonies of prairie dogs on public lands while dealing with many conflicting interests, social costs, and risks. We review the management plans that municipalities have designed to reduce conflicts by using public input, zoned management, and a variety of management techniques. Areas of difficulty and research needs are also discussed.

Restoring Ecological Integrity Of Great Rivers: Historical Hydrographs Aid In Defining Reference Conditions For The Missouri River, David L. Galat, Robin Lipkin

United States Geological Survey: Staff Publications

Restoring the ecological integrity of regulated large rivers necessitates characterizing the natural flow regime. We applied ‘Indicators of Hydrologic Alteration’ to assess the natural range of variation of the Missouri River’s flow regime at 11 locations before (1929–1948) and after (1967–1996) mainstem impoundment. The 3768 km long Missouri River was divided into three sections: upper basin least-altered from flow regulation, including the lower Yellowstone River; middle basin inter-reservoir, and lower basin channelized. Flow regulation was associated with a reduction in magnitude and duration of the annual flood pulse, an increase in magnitude and duration of annual discharge minima, a reduction …

Phylogeny Of Calostoma, The Gelatinous-Stalked Puffball, Based On Nuclear And Mitochondrial Ribosomal Dna Sequences, Brandi D. Hughey, Gerard C. Adams, Tom D. Bruns, David S. Hibbett

Department of Plant Pathology: Faculty Publications

Nucleotide sequence of the nuclear small and large subunit and the mitochondrial small and large ribosomal genes of Calostoma were used to investigate evolutionary relationships with other Basidiomycetes. In separate analyses with each gene, Calostoma was placed within the Boletales and outside the clades containing most gilled mushrooms, puffballs, stalked puffballs, earthstars, stinkhorns, and nonboletalean Gasteromycetes. This contradicted previous taxonomic hypotheses. Calostoma was most closely related to Gyroporus, Pisolithus, and Scleroderma based on maximum parsimony and maximum likelihood analysis of the mitochondrial large ribosomal gene database, which contained several representatives of most genera of the Boletales. In three …

Dissertations And Theses Sponsored By The Department Of Sociology In The University Of Nebraska-Lincoln 1905-1999: Alphabetical And Chronological Lists, Michael R. Hill

Department of Sociology: Faculty Publications

The year 2000 marks the centennial of the formal departmental organization of sociology at the University of Nebraska. The compilations presented here recognize and celebrate the achievements of hundreds of graduate students, my fellow alumni, who have completed masters theses and doctoral dissertations under the auspices of the Department of Sociology. These student works are constructive, often innovative additions to the advancement of knowledge, and several have been abridged in journal articles or published as books (cf., Hill 1988b). The doctoral dissertation, in particular, is a major rite de passage in the transition from student to intellectual (Deegan and Hill …

Holocene Environmental And Climatic Change In The Northern Great Plains As Recorded In The Geochemistry Of Sediments In Pickerel Lake, South Dakota, Walter E. Dean, Antje Schwalb

United States Geological Survey: Staff Publications

The sediments in Pickerel Lake, northeastern South Dakota, provide a continuous record of climatic and environmental change for the last 12000 yr. Sediments deposited between 12 and 6 ka (radiocarbon) show extreme variations in composition, oxygen and carbon isotopic composition of bulk carbonate, carbon isotopic composition of organic matter, and magnetic susceptibility. These variations reflect changes in sources of moisture, regional vegetation types, precipitation-evaporation balance, ground- and surface-water influx, water residence time, erosion, lake productivity, water level, and water temperature. The total carbonate content of late Pleistocene sediments steadily increased from <20% at the base of the core to as much as 80% in sediments deposited between 11 and 9 ka. By about 8 ka, the total carbonate content of the sediments had declined to about 40% where it remained with little variation for the past 8 kyr, suggesting relatively stable conditions. There are marked increases in values of δ¹³C and δ¹⁸O in bulk carbonate, …

Predicting The Probability Of Detecting Organochlorine Pesticides And Polychlorinated Biphenyls In Stream Systems On The Basis Of Land Use In The Pacific Northwest, Usa, Robert W. Black, Alan L. Haggland, Frank D. Voss

United States Geological Survey: Staff Publications

We analyzed streambed sediment and fish tissue (Cottus sp.) at 30 sites in the Puget Sound and Willamette basins in Washington and Oregon, USA, respectively, for organochlorine pesticides and polychlorinated biphenyls (PCBs). The study was designed to determine the concentrations of organochlorine pesticides and PCBs in fish tissue and sediment by land use within these basins and to develop an empirical relation between land use and the probability of detecting these compounds in fish tissue or sediment. We identified 14 organochlorines in fish tissue and sediment; three compounds were unique to either fish tissue or sediment samples. The …

Viability Of Piping Plover Charadrius Melodus Metapopulations, Jonathan H. Plissner, Susan M. Haig

United States Geological Survey: Staff Publications

The meta-population viability analysis package, vortex, was used to examine viability and recovery objectives for piping plovers Charadrius melodus, an endangered shorebird that breeds in three distinct regions of North America. Baseline models indicate that while Atlantic Coast populations, under current management practices, are at little risk of near-term extinction, Great Plains and Great Lakes populations require 36% higher mean fecundity for a significant probability of persisting for the next 100 years. Meta-population structure (i.e. the delineation of populations within the meta-population) and inter-population dispersal rates had varying effects on model results; however, spatially-structured meta-populations exhibited lower viability than …

Consequences Of Antenna Design In Telemetry Studies Of Small Passerines, Steve J. Dougill, Luanne Johnson, Paul C. Banko, Dan M. Goltz, Michael R. Wiley, John D. Semones

United States Geological Survey: Staff Publications

Entanglement and mortality of Palila (Loxioides bailleui), an endangered Hawaiian honeycreeper, occurred when birds were radio-tagged with transmitters equipped with a long, limp, solder-tipped antenna. Birds were found suspended in trees by their transmitter antenna on eight occasions. Although these birds eventually freed themselves or were freed by us, at least one bird died afterwards. For radio telemetry studies of small passerine species we recommend avoiding transmitters equipped with an antenna that is bulbous at the tip, >16 cm in length, limp, and shiny.

Single-Dilution Enzyme-Linked Immunosorbent Assay For Quantification Of Antigen-Specific Salmonid Antibody, Stewart W. Alcorn, Ronald J. Pascho

United States Geological Survey: Staff Publications

An enzyme-linked immunosorbent assay (ELISA) was developed on the basis of testing a single dilution of serum to quantify the level of antibody to the p57 protein of Renibacterium salmoninarum in sockeye salmon (Oncorhynchus nerka). The levels of antibody were interpolated from a standard curve constructed by relating the optical densities (OD) produced by several dilutions of a high-titer rainbow trout (O. mykiss) antiserum to the p57 protein. The ELISA OD values produced by as many as 36 test sera on each microplate were compared with the standard curve to calculate the antigen-specific antibody activity. Repeated …

Natural And Anthropogenic Influences On The Distribution Of The Threatened Neosho Madtom In A Midwestern Warmwater Stream, Mark L. Wildhaber, Vernon M. Tabor, Daniel W. Mulhern, Kenneth L. Powell, Scott P. Sowa

United States Geological Survey: Staff Publications

We attempted to discern the contributions of physical habitat, water chemistry, nutrients, and contaminants from historic lead-zinc mining activities on the riffle-dwelling benthic fish community of the Spring River, a mid western warm water stream that originates in Missouri and flows into Kansas and Oklahoma. The Spring River has a fish community that includes the Neosho madtom Noturus placidus, a species federally listed as threatened. Although anthropogenic factors such as contaminants limited populations and densities of fishes, an integrated assessment of natural and anthropogenic factors was necessary to effectively estimate the influence of the latter. Fish populations in the …

A New Eimeria Sp. From The Plumbeous Central American Caecilian, Dermophis Mexicanus (Amphibia: Gymnophiona) From Volcán, Tajumulco, Department Of San Marcos, Guatemala, Ingrid M. Asmundsson, Jonathan A. Campbell, Donald W. Duszynski

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Fresh fecal samples from 5 caecilians (Dermophis mexicanus) were collected and examined for coccidia in the summer of 1998. The caecilians were collected in the Department of San Marcos, Guatemala. Two of the 5 (40%) specimens of caecilians contained an Eimeria species that is described here as new. This represents the first coccidia described from a gymnophionian host. Sporulated oocysts are spheroidal to subspheroidal, 19.5 × 17.7 (16–23 × 15–21) μm, micropyle and oocyst residuum are absent, and 3 (or more) polar granules are always present. Sporocysts are ovoidal, 11.0 × 7.2 (10–12 × 6–9); a Stieda body …

Coccidia (Apicomplexa: Eimeriidae) Of The Mammalian Order Insectivora, Donald W. Duszynski, Steve J. Upton

Harold W. Manter Laboratory of Parasitology: Faculty Publications

The coccidia (protistan phylum Apicomplexa Levine, 1970) comprise a large group of obligate intracellular parasites commonly found in all classes of vertebrate hosts and in some invertebrates. This review focuses on those species within the Eimeriidae because they are among the most prevalent and speciose of all parasite groups, and there is no taxonomic summation currently available for those species that infect insectivores. All published species descriptions in the genera Cyclospora, Eimeria and Isospora that infect insectivores are reviewed and evaluated. Some of the named species are invalid, either because rules concerning the naming of new species (International …