Digital Commons Network^™

Use Of Flow Cytometry, Fluorescence Microscopy, And Pcr-Based Techniques To Assess Intraspecific And Interspecific Matings Of Armillaria Species, Mee-Sook Kim, Ned B. Klopfenstein, Geral I. Mcdonald, Kathiravetpillai Arumuganathan, Anne K. Vidaver

Department of Plant Pathology: Faculty Publications

For assessments of intraspecific mating using flow cytometry and fluorescence microscopy, two compatible basidiospore-derived isolates were selected from each of four parental basidiomata of North American Biological Species (NABS) X. The nuclear status in NABS X varied with basidiospore-derived isolates. Nuclei within basidiospore-derived isolates existed as haploids, diploids (doubled haploids), or a mixture of haploids and diploids (doubled haploids). Depending on the nuclear status of the basidiospore-derived lines of NABS X, intraspecifically mated cultures can exist as diploids or tetraploids, and possibly triploids or aneuploids under in vitro conditions. Based on previous in vitro mating studies, seven basidiospore isolates were …

Relative Kinematics Of The Rib Cage And Abdomen During Speech And Nonspeech Behaviors Of 15-Month-Old Children, Christopher A. Moore, Tammy J. Caulfield, Jordan R. Green

Department of Special Education and Communication Disorders: Faculty Publications

Speech motor control emerges in the neurophysiologic context of widely distributed, powerful coordinative mechanisms, including those mediating respiratory function. It is unknown, however, whether developing children are able to exploit the capabilities of neural circuits controlling homeostasis for the production of speech and voice. Speech and rest breathing were investigated in eleven 15-month-old children using inductance plethysmography (Respitrace). Rib cage and abdominal kinematics were studied using a time-varying correlational index of thoracoabdominal coupling (i.e., reflecting the synchrony of movement of the rib cage and abdomen) as well as simple classification of the moment-to-moment kinematic relationship of these two functional components …

Nebline, February 2001

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

We’re Here When You May Need Us Most! http://lancaster.unl.edu
It's a Hit!
“But I don’t have a computer”
What Does the Future Hold for This Web Site?
Pruning Mature Deciduous Shrubs
2001 All America Selection
Basil for Your Garden
We need your help!
Beginning Beekeeping Classes: Kearney and Omaha
Editors Comment
Prevent Rabbit Damage to Trees and Shrubs
Computerized Financial Recordkeeping
What is Organic? Now We Know
New EPA Regulations
Display Tips for Farmers Markets
Egg Eating by Chickens
Lawn Care Management Focus of March UNL Workshop
It’s Garden Seed Catalog Time
Top Ten List
Healthy Eating
Helping of Holiday …

Water Current, Volume 33, No. 1. February 2001.

Water Current Newsletter

• Researchers Pool Talents To Protect Nebraska’s Premier Trout Fishing Locale by Steve Ress

• From the Director

• Meet the Faculty

• LB699 As A Sales Tax For Natural Resources Programs by Dean E. Edson

• 30th Annual Water Conference Will be Groundwater Quality Monitoring Workshop

• Water News Briefs

Predicting Forage Quality In Switchgrass And Big Bluestem, Rob Mitchell, John Fritz, Ken Moore, Lowell E. Moser, Ken Vogel, Daren D. Redfearn, David Wester

Department of Agronomy and Horticulture: Faculty Publications

Predicting forage quality would help producers scheduled hay harvesting to obtain desired hay quality. Our objective was to determine if growing degree day (GDD), day of the year (DOY), mean stage count (MSC), and mean stage weight (MSW) could be used to predict in vitro digestible dry matter (IVDDM), crude protein (CP), and neutral-detergent fiber (NDF) of ‘Trailblazer’ switchgrass (Panicum virgatum.) and ‘Pawnee’ big bluestem (Andropogon gerardii Vitman) grown in Nebraska and Kansas. This field study was conducted from 1990 to 1993 at Mead, NE on Typic Argiudoll soils and from 1992 to 1993 at Manhattan, KS …

Characterization Of The Laminated Layer Of In Vitro Cultivated Echinococcus Vogeli Metacestodes, Katrin Ingold, Wenjuan Dai, Robert L. Rausch, Bruno Gottstein, Andrew Hemphill

Harold W. Manter Laboratory of Parasitology: Faculty Publications

The metacestode (larval) stages of the cestode parasites Echinococcus vogeli and E. multilocularis were isolated from the peritoneal cavity of experimentally infected C57BL/6 mice and were cultured in vitro for a period of up to four months under conditions normally applied for the in vitro cultivation of E. multilocularis metacestodes. In contrast to E. multilocularis, E. vogeli did not exhibit extensive exogenous budding and proliferation but increased in size with a final diameter of up to 10 mm. Most metacestodes contained protoscoleces, singly or in groups, either associated with brood capsules or growing directly out of the germinal layer. …

The History Of South American Tropical Precipitation For The Past 25,000 Years, Paul A. Baker, Geoffrey O. Seltzer, Sherilyn C. Fritz, Robert B. Dunbar, Matthew J. Grove, Pedro M. Tapia, Scott L. Cross, Harold D. Rowe, James P. Broda

Department of Earth and Atmospheric Sciences: Faculty Publications

Long sediment cores recovered from the deep portions of Lake Titicaca are used to reconstruct the precipitation history of tropical South America for the past 25,000 years. Lake Titicaca was a deep, fresh, and continuously overflowing lake during the last glacial stage, from before 25,000 to 15,000 calibrated years before the present (cal yr B.P.), signifying that during the last glacial maximum (LGM), the Altiplano of Bolivia and Peru and much of the Amazon basin were wetter than today. The LGM in this part of the Andes is dated at 21,000 cal yr B.P., approximately coincident with the global LGM. …

Understanding Vole Problems In Direct Seeding — Strategies For Management, Gary W. Witmer, Kurt C. Vercauteren

United States Department of Agriculture Wildlife Services: Staff Publications

Crop fields can provide habitat to a variety of wildlife and crop damage can result (Wywialowski 1996, 1998; Conover 1998). Among the vertebrates, damage can occur from numerous species of birds and mammals. Worldwide concern, however, has focused on rodents and a large number of species cause substantial agricultural losses each year (Witmer et al. 1995). After the advent of effective herbicides and "clean farming" practices in North America, however, many rodent problems became insignificant (Hines and Hygnstrom 2000). This is, in large part, because the fields were plowed each year, disrupting burrows and removing ground cover. The fields often …

Monitoring Changes In Feral Swine Abundance And Spatial Distribution, Richard M. Engeman, Bernice Constantin, Mark Nelson, John Woolard, Jean Bourassa

United States Department of Agriculture Wildlife Services: Staff Publications

Swine (Sus scrofa) have been introduced in many places throughout the world, and in many places they adversely affect the environment, economically impact agriculture, and/or harbor diseases transmittable to domestic livestock or humans. An easily applied method to assess their abundance is an important need for their management. To monitor efficacy of a swine control program in Florida, data from passive tracking plots provide an index of feral swine abundance. The same track data coupled with plot locations to numerically describe the spatial pattern of swine activity gave an index of pervasiveness, and a simple rate of interception …

A Review Of The Hazards And Mitigation For Airstrikes From Canada Geese In The Anchorage, Alaska Bowl, Darryl L. York, Richard M. Engeman, John L. Cummings, Corey L. Rossi, David R. Sinnett

United States Department of Agriculture Wildlife Services: Staff Publications

Bird strikes to aircraft are a globally pervasive safety and economic concern. In particular, the Anchorage, Alaska area holds substantial risk for destructive collisions between birds and aircraft. An international airport, a municipal airport, a seaplane base, and a U.S. Air Force base are situated throughout the area. Anchorage also has a burgeoning population of Canada geese (Branta canadensis parvipes), one of the species identified as posing the greatest airstrike hazards. A variety of methods are applied and a variety of research has been conducted to reduce the potential for airstrikes with geese around Anchorage.We review the nature …

Understanding Vole Problems In Direct Seeding-Strategies For Management, Gary W. Witmer, Kurt C. Vercauteren

United States Department of Agriculture Wildlife Services: Staff Publications

Crop fields can provide habitat to a variety of wildlife and crop damage can result (Wywialowski 1996,1998; Conover 1998). Among the vertebrates, damage can occur from numerous species of birds and mammals. Worldwide concern, however, has focused on rodents and a large number of species cause substantial agriculture losses each year (Witmer et al, 1995). After the advent of effective herbicides and "clean farming" practices in North America, however, many rodent problems became insignificant (Hines and Hygnstrom 2000). This is, in large part, because the fields were plowed each year, disrupting burrows and removing ground cover. The fields often lay …

Habitat Characteristics Around Fall Blackbird Roosts, Mark W. Lutman, George M. Linz, William J. Bleier

United States Department of Agriculture Wildlife Services: Staff Publications

In the fall, blackbirds form large flocks and roost overnight in cattail marshes. These blackbirds cause millions of dollars in damage to ripening sunflower fields in North Dakota, South Dakota, and Minnesota. By knowing the habitat characteristics around fall blackbird roosts sites, management officials may be able to predict future roost locations and then implement control techniques to help reduce blackbird damage to sunflower fields. We present data on the available habitat around blackbird roosts to determine if there is a relationship between the habitat and fall roost sites.

Husker Feed Grains And Soybean Conference, John Owens

John Owens: Speeches and Appearances

Thank you for inviting me to speak with you today. It is a pleasure to be here. I look forward to working with you in the days ahead; in fact, I look forward “to attending” this conference in the future and knowing many of you by name.

Evaluation Of Gel Filtration Resins For The Removal Of Pcr-Inhibitory Substances From Soils And Sediments, Daniel N. Miller

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

A variety of gel filtration resins (Sephadex G200 and G150; Sepharose 6B, 4B and 2B; Bio-Gel P100, P200; and Toyopearl HW 55, HW 65, and HW 75) were evaluated for their efficacy in removing PCR-inhibitory substances from feedlot soil DNA crude extracts using gravity-flow disposable columns. Sepharose resins demonstrated the best properties for DNA purification when compared to other gel filtration resins, and Sepharose 2B was the most efficient purification resin based upon flow rate and the elution of DNA and humic acids from the columns. A method for purifying large solution volumes of DNA extract economically was also developed …

Coyote Responses To Changing Jackrabbit Abundance Affect Sheep Predation, L. Charles Stoddart, Richard E. Griffiths, Frederick F. Knowlton

United States Department of Agriculture Wildlife Services: Staff Publications

Domestic sheep ranchers generally perceive abundances of natural prey and coyotes (Canis latrans) as important factors affecting coyote predation rates on sheep. To determine the effect of a changing natural prey base on coyote predation rates, we estimated coyote density and predation rates on ewes and lambs during part of 1 cycle of black-tailed jackrabbit (Lepus californicus) abundance on a 2,300 km² area of the Idaho National Engineering Laboratory in south-central Idaho from 1979–1985. We used 100, 1.6-km scat collection lines and 80, 1.6-km flushing transects to assess coyote and jackrabbit densities, respectively. Ewe and …

Agriculture Builders Of Nebraska Inc., John Owens

John Owens: Speeches and Appearances

It’s a pleasure to be able to speak with you today. I want you to know that Virginia and I are delighted to be in Nebraska. And I’m especially happy to have arrived in the state at a time where I can, on my 7th day on the job, learn so much about UNL just by reading the state’s largest newspaper.

Genetic And Phenotypic Parameters For Dietary Selection Of Mountain Big Sagebrush (Artemisia Tridentata Nutt. Ssp. Vaseyana [Rydb] Beetle) In Rambouillet Sheep, G. D. Snowder, J. W. Walker, K. L. Launchbaugh, L. Dale Van Vleck

Department of Animal Science: Faculty Publications

The heritability of diet selection for mountain big sagebrush (Artemisia tridentata Nutt. ssp. vaseyana [Rydb] Beetle) by grazing sheep was estimated from fecal samples collected from 549 Rambouillet ewes. Fecal samples were collected in September and October during 1996 and 1997 from free-grazing ewes on intermountain sagebrush-bunchgrass rangelands at the U.S. Sheep Experiment Station in Idaho. The total number of fecal samples was 1,949. Fecal samples were evaluated for composition of big sagebrush by near-infrared spectroscopy. Percentage of sagebrush in the diet was less in September than in October (21.6 vs 31.7%, respectively). Single-trait and bivariate derivative-free REML analyses …

Atomically Resolved Single-Walled Carbon Nanotube Intramolecular Junctions, Min Ouyang, Jin-Lin Huang, Chin Li Cheung, Charles M. Lieber

Barry Chin Li Cheung Publications

Intramolecular junctions in single-walled carbon nanotubes are potentially ideal structures for building robust, molecular-scale electronics but have only been studied theoretically at the atomic level. Scanning tunneling microscopy was used to determine the atomic structure and electronic properties of such junctions in single-walled nanotube samples. Metal-semiconductor junctions are found to exhibit an electronically sharp interface without localized junction states, whereas a more diffuse interface and low-energy states are found in metal-metal junctions. Tight-binding calculations for models based on observed atomic structures show good agreement with spectroscopy and provide insight into the topological defects forming intramolecular junctions. These studies have important …

Genetic And Biological Variation In Equine Infectious Anemia, Michael Belshan, Prasith Baccum, J. Lindsay Oaks, Brett A. Sponseller, Sean C. Murphy, James Cornette, Susan Carpenter

Nebraska Center for Virology: Faculty Publications

Genetic and biological variation in the regulatory protein Rev of equine infectious anemia virus (EIAV) were examined throughout a clinically dynamic disease course of an experimentally infected pony. Following infection with the virulent EIAV_Wyo, the pony underwent a variable disease course, including an acute fever episode at 12 days postinfection (DPI), multiple recurrent fever episodes until 135 DPI, a prolonged subclinical period, and two late fever episodes. Viral RNA was isolated from the inoculum and sequential sera samples, and the rev exon 2/gp45 overlapping ORFs were amplified, cloned, and sequenced. Novel variants were found throughout infection, and genetic …

Manure Impacts On Interrill Erosion, John E. Gilley, Bahman Eghball, J. M. Blumenthal, David D. Baltensperger

Department of Agricultural and Biological Systems Engineering: Presentations and White Papers

This study was conducted to measure runoff and erosion from interrill areas as affected by the long-term application of manure and fertilizer to a Tripp sandy loam soil located near Mitchell, Nebraska. Soil that had been removed from the top 0.1 m of the soil profile was placed in a 1 m² soil pan. Rainfall was then applied to the soil pan during initial and wet simulation events. Total runoff was similar on the manure and no-manure treatments. The long-term application of manure (55 years) at a rate of 27 Mg ha^-1 (wet basis) per year did not …

Review Of Hazel Rue, Bomby The Bombardier Beetle, Brett C. Ratcliffe

University of Nebraska State Museum: Entomology Papers

Well, I thought that the style of brainwashing seen in this revisionist book went out with the 1950s Cold War era. However, the Institute for Creation Research demonstrates that brainwashing is alive and well as it continues to wage its own cold war against reason in order to replace it with superstition. In this highly disjointed little book, the target is young children, which makes the authors’ sin of deliberate ignorance even more reprehensible. Educating children about the wonders of nature is a delightful endeavor, but here it is a vehicle for blatantly meshing pseudo-natural history with creationist dogma that …

Review Of Cetoniimania, A Publication Devoted To Cetoniinae, Valginae, And Trichiinae., Brett C. Ratcliffe

University of Nebraska State Museum: Entomology Papers

This beautifully produced publication devoted to Cetoniinae (Scarabaeidae) and their relatives is the brainchild of Robert Alexis (editor) and Michel Delpont (director of publication). Volume 1 (No. 1, 52 pp) was printed in September 2000 and contained articles clarifying the status of Potosia cuprea Fabr. (with magnificent color illustrations of beetles, habitats, and range maps), collecting on Krk island in Yugoslavia, the cetoniine fauna of Lesbos island in Greece, and Cetoniidarum Specierum Lexicon, which is a supplemental but integral part of Cetoniimania in which new taxa are described in both French and English (again, with excellent color illustrations and line …

Insulin-Like Growth Factor Binding Proteins In The Bovine Anterior Pituitary, Andrew J. Roberts, Richard N. Funston, Gary E. Moss

Roman L. Hruska U.S. Meat Animal Research Center: Reports

and 2Department of Animal Science, University of Wyoming, Laramie, WY Endocrine, vol. 14, no. 3, 399–406, April 2001 0969–711X/01/14:399–406/$12.00 © 2001 by Humana Press Inc. All rights of any nature whatsoever reserved. 399 Insulin-like growth factor binding proteins (IGFBPs) were characterized in bovine anterior pituitary tissue, pituitary conditioned media, and serum collected during the preovulatory and early luteal phases of the estrous cycle. Effects of in vitro treatments of pituitaries with luteinizing hormone–releasing hormone (LHRH), estradiol, and progesterone on IGFBP secretion were also evaluated. Predominant IGFBPs detected in anterior pituitary tissue by immunoprecipitation, ligand blotting, and Northern blotting were IGFBP-5 …

Abrogation Of Disease Development In Plants Expressing Animal Antiapoptotic Genes, M. B. Dickman, Y. K. Park, T. Oltersdorf, W. Li, T. Clemente, Roy C. French

Department of Plant Pathology: Faculty Publications

An emerging topic in plant biology is whether plants display analogous elements of mammalian programmed cell death during development and defense against pathogen attack. In many plant– pathogen interactions, plant cell death occurs in both susceptible and resistant host responses. For example, specific recognition responses in plants trigger formation of the hypersensitive response and activation of host defense mechanisms, resulting in restriction of pathogen growth and disease development. Several studies indicate that cell death during hypersensitive response involves activation of a plant-encoded pathway for cell death. Many susceptible interactions also result in host cell death, although it is not clear …

Three Distinct Mechanisms Facilitate Genetic Isolation Of Sympatric Wheat Streak Mosaic Virus Lineages, Jeffrey Hall, Roy C. French, Gary L. Hein, Thomas Jack Morris, Drake Stenger

Department of Plant Pathology: Faculty Publications

Cross-protection and vector transmission bottlenecks have been proposed as mechanisms facilitating genetic isolation of sympatric viral lineages. Molecular markers were used to monitor establishment and resolution of mixed infections with genetically defined strains of wheat streak mosaic virus (WSMV). Two closely related WSMV strains from the U.S. (Type and Sidney 81) exhibited reciprocal cross-protection in wheat, confirming this classic phenomenon as a mechanism of genetic isolation. In contrast, cross-protection between either U.S. strain and the divergent El Batan 3 strain from Mexico was unilateral, erratic, and only partially effective. Distribution of WSMV strains within individual leaves of plants supporting a …

Intsormil 2001 Annual Report, John M. Yohe, Thomas Crawford Jr., Joan Frederick, Dorothy Stoner

INTSORMIL Impacts and Bulletins

Presently, worldwide, more than 800 million people are hungry and over one billion are desperately poor, and food demand is increasing rapidly. The majority of poor live in rural areas in developing countries and agricultural and food systems development is vital to economic growth; improving environmental quality; strengthening nutrition, health and child survival; improving the status of women; and promoting democratization. It is estimated that, between 1980 and 2030, the population ofow- and middle-income countries will more than double-to seven billion, compared with one billion for high-income countries. In the next 35 years, 2.5 billion people will be added to …

2001 Platte River Basin Ecosystem Symposium Proceedings

Water Current Newsletter

Over 75 people braved the wintry weather to attend the Eleventh Platte River Basin Ecosystem Symposium that convened in Kearney on 27 February 2001. Icy roads lead to cancellation of only 2 papers. Seventeen papers were presented and 5 posters were on display. Nebraska Public Radio and NTV News covered the event. The presentations were excellent and participants were well satisfied with not only the program but also the food. This year the symposium broadened its charge to include more discussion on community-based environmental protection, sustainable development, and policy-making. The Symposium was sponsored by the University of Nebraska Cooperative Extension …

Fluxnet: A New Tool To Study The Temporal And Spatial Variability Of Ecosystem-Scale Carbon Dioxide, Water Vapor, And Energy Flux Densities, Dennis Baldocchi, Eva Falge, Lianhong Gu, Richard Olson, David Hollinger, Steve Running, Peter Anthoni, Ch. Bernhofer, Kenneth Davis, Robert Evans, Jose Fuente, Allen Goldstein, Gabriel Katul, Beverly Law, Xuhui Lee, Yadvinder Malhi, Tilden Meyers, William Munge, Walt Oechel, K.T. Paw U, Kim Pilegaard, H.P. Schmid, Riccardo Valentini, Shashi Verma, Timo Vesala, Kell Wilson, Steve Wofsy

School of Natural Resources: Faculty Publications

FLUXNET is a global network of micrometeorological flux measurement sites that measure the exchanges of carbon dioxide, water vapor, and energy between the biosphere and atmosphere. At present over 140 sites are operating on a long-term and continuous basis. Vegetation under study includes temperate conifer and broadleaved (deciduous and evergreen) forests, tropical and boreal forests, crops, grasslands, chaparral, wetlands, and tundra. Sites exist on five continents and their latitudinal distribution ranges from 70°N to 30°S.

FLUXNET has several primary functions. First, it provides infrastructure for compiling, archiving, and distributing carbon, water, and energy flux measurement, and meteorological, plant, and soil …

Some Perspectives On Recent In Situ Air Temperature Observations: Modeling The Microclimate Inside The Radiation Shields*, X. Lin, Kenneth Hubbard, Elizabeth A. Walter-Shea, James Brandle, G.E. Meyer

School of Natural Resources: Faculty Publications

Air temperature measurement has inherent biases associated with the particular radiation shield and sensor deployed. The replacement of the Cotton Region Shelter (CRS) with the Maximum–Minimum Temperature System (MMTS) and the introduction of Automated Surface Observing System (ASOS) air temperature observing systems during the NWS modernization introduced bias shifts in federal networks that required quantification. In rapidly developing nonfederal networks, the Gill shield temperature systems are widely used. All of these systems house an air temperature sensor in a radiation shield to prevent radiation loading on the sensors; a side effect is that the air temperature entering a shield is …

Impacts Of Predation On Northern Bobwhite And Scaled Quail, Dale Rollins, John P. Carroll

School of Natural Resources: Faculty Publications

Northern bobwhite (Colinus virginianus) and scaled quail (Callipepla squamata) populations have declined throughout most of their distribution, and these declines have become more dramatic in recent years. In this review, we examine the role of predation in quail management. Predation is the major source of nest loss and of mortality for young and adult quail. Mean nest success across studies reviewed was 28%. Estimates of annual survival rates have varied from 5 to 26% for radiotelemetry studies and from 15 to 30% based on age-ratio studies. Breeding season survival estimates ranged from 13 to 51% in telemetry …