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Genomewide Identification Of Pseudomonas Syringae Pv.Tomato Dc3000 Promoters Controlled By The Hrpl Alternative Sigma Factor, Derrick E. Fouts, Robert B. Abramovitch, James R. Alfano, Angela M. Baldo, C. Robin Buell, Samuel Cartinhour, Arn K. Chatterjee, Mark D'Ascenzo, Michelle L. Gwinn, Sondra G. Lazarowitz, Na-Chun Lin, Gregory B. Martin, Amos H. Rehm, David J. Schneider, Karin V. Van Dijk, Xiaoyan Tang, Alan Collmer

Center for Plant Science Innovation: Faculty Publications

The ability of Pseudomonas syringae pv. tomato DC3000 to parasitize tomato and Arabidopsis thaliana depends on genes activated by the HrpL alternative sigma factor. To support various functional genomic analyses of DC3000, and specifically, to identify genes involved in pathogenesis, we developed a draft sequence of DC3000 and used an iterative process involving computational and gene expression techniques to identify virulence-implicated genes downstream of HrpLresponsive promoters. Hypersensitive response and pathogenicity (Hrp) promoters are known to control genes encoding the Hrp (type III protein secretion) machinery and a few type III effector proteins in DC3000. This process involved (i) identification of …

Ianr's Contributions To Nebraska's Future, John Owens

John Owens: Speeches and Appearances

One in four Nebraskans depends upon agriculture for employment. That fact found in the 2001 Nebraska-Agriculture Fact Card, a cooperative effort of the Nebraska Bankers Association, the Nebraska AgRelations Council, and the Nebraska Department of Agriculture, Underscores the importance of agriculture to Nebraska, and the importance of the work conducted in the Institute of Agriculture and Natural Resources.

Effects Of Herbivory On The Reproductive Effort Of 4 Prairie Perennials, Erica Spotswood, Kate L. Bradley, Johannes M. H. Knops

School of Biological Sciences: Faculty Publications

Background: Herbivory can affect every aspect of a plant's life. Damaged individuals may show decreased survivorship and reproductive output. Additionally, specific plant species (legumes) and tissues (flowers) are often selectively targeted by herbivores, like deer. These types of herbivory influence a plant's growth and abundance. The objective of this study was to identify the effects of leaf and meristem removal (simulated herbivory within an exclosure) on fruit and flower production in four species (Rhus glabra, Rosa arkansana, Lathyrus venosus, and Phlox pilosa) which are known targets of deer herbivory.

Results: Lathyrus never flowered or went to …

First Records Of Loosely Coiled Valve Snail In North Dakota, Bruce A. Hanson, Ned H. Euliss Jr., David M. Mushet

United States Geological Survey, Northern Prairie Wildlife Research Center: Publications

On 14 June 1994 we collected a living specimen of Valvata lewisii morph ontariensis at the Cottonwood Lake Study Area, Stutsman County, North Dakota. The voucher specimen is housed in the aquatic invertebrate collection at Northern Prairie Wildlife Research Center. We captured the specimen in a funnel trap (Swanson 1978) in the shallow marsh zone of a seasonal wetland. This is in contrast to the typical habitats for this species, which are large lakes and rivers (Clarke 1981). Water depth at the site where we collected Valvata lewisii ontariensis was 33 cm. Shallow marsh plants in the wetland included water …

Comparison Of Models For Estimation Of Genetic Parameters For Mature Weight Of Hereford Cattle, J. M. Rumph, R. M. Koch, K. E. Gregory, L. V. Cundiff, L. Dale Van Vleck

Department of Animal Science: Faculty Publications

Genetic parameters of mature weight are needed for effective selection and genetic evaluation. Data for estimating these parameters were collected from 1963 to 1985 and consisted of 32,018 mature weight records of 4,175 Hereford cows that were in one control and three selection lines that had been selected for weaning weight, for yearling weight, or for an index combining yearling weight and muscle score for 22 yr. Several models and subsets of the data were considered. The mature weight records consisted of a maximum of three seasonal weights taken each year, at brand clipping (February and March), before breeding (May …

Expression Of Active Human Factor Ix In Mammary Tissue And Of Milk Non Human Transgenic Mammals , William H. Velander, William N. Drohan

Papers in Biotechnology

Recombinant Factor IX characterized by a high percentage of active protein can be obtained in the milk of transgenic animals that incorporate chimeric DNA molecules according to the present invention. Transgenic animals of the present invention are produced by introducing into developing embryos DNA that encodes Factor IX, such that the foreign DNA is stably incorporated in the DNA of germ line cells of the mature animal. Particularly efficient expression was accomplished using a chimeric construct comprising a mammary gland specific promoter, Factor IX cDNA that lacked the complete or any portion of the 5'-untranslated and 3'-untranslated region, which is …

Population Increases Of Large Bird Species In Relation To Impact Standards For Aircraft Engines And Airframes, Richard A. Dolbeer

United States Department of Agriculture Wildlife Services: Staff Publications

Bird-aircraft collisions (bird strikes) are an increasing safety and economic concern to the USA civil aviation industry, costing over $400 million each year. One approach to reducing risks associated with strikes is to require commercial aircraft components to meet certain standards of safe performance in the event of a bird strike. The Federal Aviation Administration has developed airworthiness standards for airframes, windshields and engines using a single 4-lb (1.82-kg) bird mass as the maximum that must be tested (with the exception of a single 8-lb bird for the empennage, 6-lb bird for certain mid-sized engines that may be developed in …

Characterization Of Vadose Zone Sediment: Uncontaminated Rcra Borehole Core Samples And Composite Samples, R. J. Serne, B. N. Bjornstad, H. T. Schaef, B. A. Williams, D. C. Lanigan, D. G. Horton, R. E. Clayton, A. V. Mitroshkov, V. L. Legore, M. J. O’Hara, C. F. Brown, K. E. Parker, I. V. Kutnyakov, J. N. Serne, G. V. Last, Steven Smith, C. W. Lindenmeier, John M. Zachara, D. B. Burke

United States Department of Energy: Publications

The overall goal of the of the Tank Farm Vadose Zone Project, led by CH2M HILL Hanford Group, Inc., is to define risks from past and future single-shell tank farm activities. To meet this goal, CH2M HILL Hanford Group, Inc. asked scientists from Pacific Northwest National Laboratory to perform detailed analyses on vadose zone sediment from within the S-SX Waste Management Area. This report is one in a series of four reports to present the results of these analyses. Specifically, this report contains all the geologic, geochemical, and selected physical characterization data collected on vadose zone sediment recovered from Resource …

Relationship Of Effective To Census Size In Fluctuating Populations, Steven Kalinowski, Robin Waples

United States Department of Commerce: Staff Publications

The effective size of a population (N_e) rather than the census size (N) determines its rate of genetic drift. Knowing the ratio of effective to census size, N_e / N , is useful for estimating the effective size of a population from census data and for examining how different ecological factors influence effective size. Two different multigenerational ratios have been used in the literature based on either the arithmetic mean or the harmonic mean in the denominator. We clarify the interpretation and meaning of these ratios. The arithmetic mean N_e / N ratio …

Long-Term Manure And Fertilizer Application Effects On Phosphorus And Nitrogen In Runoff, Bahman Eghball, John E. Gilley, David D. Baltensperger, J. M. Blumenthal

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Long–term manure and fertilizer applications to a soil can increase phosphorus (P) and nitrogen (N) transport in runoff. This study was conducted to determine P and N transport in runoff following long–term (since 1953) manure and fertilizer applications. Duplicate soil samples (32) were collected in 1998 from the top 0.1 m of selected plots of a long–term manure and fertilizer applications field experiment and later placed in 1 m² soil pans in the laboratory. Manure and fertilizer were mixed with 16 of the soil samples, while no treatment was applied to the other half (long–term residual effect). Simulated rainfall …

Ard News February 2002

Agricultural Research Division: News and Annual Reports

CONTENTS:
Comments from the Dean
UNL Indicators of Institutional Quality
Distribution of Indirect Cost Facilities Projects Update
Priority Program Enhancement Proposals
North Central SARE Regional Coordinator
Scholarly Enhancement Program in CHRFS
Food and Agricultural Science Exhibition
Grants and Contracts Received December 2001 and January 2002
Proposals Submitted for Federal Grants

Visual Predators Select For Crypticity And Polymorphism In Virtual Prey, Alan B. Bond, Alan Kamil

Papers in Behavior in Biological Sciences

Cryptically colored animals commonly occur in several distinct pattern variants. Such phenotypic diversity may be promoted by frequency-dependent predation, in which more abundant variants are attacked disproportionately often, but the hypothesis has never been explicitly tested. Here we report the first controlled experiment on the effects of visual predators on prey crypticity and phenotypic variance, in which blue jays (Cyanocitta cristata) searched for digital moths on computer monitors. Moth phenotypes evolved via a genetic algorithm in which individuals detected by the jays were much less likely to reproduce. Jays often failed to detect atypical cryptic moths, confirming frequency- …

Dictyostelium Lvsb Mutants Model The Lysosomal Defects Associated With Chediak-Higashi Syndrome, Ed Harris, Ning Wang, Wei-I Wu, Alisha Weatherford, Arturo De Lozanne, James Cardelli

Department of Biochemistry: Faculty Publications

Chediak-Higashi syndrome is a genetic disorder caused by mutations in a gene encoding a protein named LYST in humans (“lysosomal trafficking regulator”) or Beige in mice. A prominent feature of this disease is the accumulation of enlarged lysosome-related granules in a variety of cells. The genome of Dictyostelium discoideum contains six genes encoding proteins that are related to LYST/Beige in amino acid sequence, and disruption of one of these genes, lvsA (large volume sphere), results in profound defects in cytokinesis. To better understand the function of this family of proteins in membrane trafficking, we have analyzed mutants disrupted in lvsA, …

Education And Policy In The New Latino Diaspora, Edmund T. Hamann, Stanton Wortham, Enrique G. Murillo Jr.

Department of Teaching, Learning, and Teacher Education: Faculty Publications

Increasing numbers of Latinos (many immigrant, and some from elsewhere in the United States) are settling both temporarily and permanently in areas of the United States that have not traditionally been home to Latinos-for example, North Carolina, Maine, Georgia, Indiana, Arkansas, rural Illinois, and near resort communities in Colorado.' Enrique Murillo and Sofia Villenas have called this the New Latino Diaspora (Murillo and Vienas, 1997). Newcomer Latinos are confronted with novel challenges to their senses of identity, status, and community. Instead of arriving in settings, like the Southwest, where Latinos have lived for centuries, those in the New Latino Diaspora …

Extended Visions January-February 2002

Extended Visions Newsletter (ARDC)

No abstract provided.

Nebline, February 2002

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

2002 is 4-H Centennial
2002 All America Selections
West Nile Virus Expected in Nebraska This Summer
Bugs Coming out of Firewood? Don’t Panic
Invasion of the Tiny Mites
Beginning Beekeeping Workshop
Rats (and Mice) ... Did you Know That ...
Farmers Tax Guides Available
Safely Feeding Moldy Hay Requires Observation
Renting Livestock Facilities
Fertilizing with Phosphorus
Fertilizing Crop Land with Biosolids
Landscape Lighting
Excess Solids May Harm Septic Systems
Pruning Overgrown Lilacs
Working with the Right Tools for Pruning
Add a Little Spice (and Herbs) to Your Life!
Healthy Eating: Enjoy Nebraska Foods
What Does 65 Pounds of Sugar Have …

Water Current, Volume 34, No. 1. February 2002

Water Current Newsletter

• UNL Chemistry Laboratory Analyzes, Teaches Groundwater’s Story by Steve Ress

• From the Director

• Meet the Faculty

• Summer Water and Natural Resources Tour Follows North Platte River

• April Water Faculty Forum in Lincoln

• Kansas v. Nebraska and Colorado by Don Blankenau

• Community Options for Wellhead Protection Areas by J. David Aiken

• Water News Briefs

• Discarded Computers and Cell Phones Pose Potential Risk to Groundwater

Functional Characterization And Localization Of The Aspergillus Nidulan Formin Sepa, Kathryn E. Sharpless, Steven D. Harris

Department of Plant Pathology: Faculty Publications

Formins are a family of multidomain scaffold proteins involved in actin-dependent morphogenetic events. In Aspergillus nidulans, the formin SEPA participates in two actin-mediated processes, septum formation and polarized growth. In this study, we use a new null mutant to demonstrate that SEPA is required for the formation of actin rings at septation sites. In addition, we find that a functional SEPA::GFP fusion protein localizes simultaneously to septation sites and hyphal tips, and that SEPA colocalizes with actin at each site. Using live imaging, we show that SEPA localization at septation sites and hyphal tips is dynamic. Notably, at septation …

Book Review: Interrelationships Of The Platyhelminthes, Scott Lyell Gardner

Harold W. Manter Laboratory of Parasitology: Faculty Publications

A review by Scott L. Gardner of Interrelationships of the Playthelminths by D. T. Littlewood and R. A. Bray (London: Taylor & Francis, 2001; 365 p.; ISBN: 0748409033).

The Sequential Development Of Jaw And Lip Control For Speech, Jordan R. Green, Christopher A. Moore, Kevin J. Reilly

Department of Special Education and Communication Disorders: Faculty Publications

Vertical displacements of the upper lip, lower lip, and jaw during speech were recorded for groups of 1-, 2-, and 6-year-olds and adults to examine if control over these articulators develops sequentially. All movement traces were amplitude- and time-normalized. The developmental course of upper lip, lower lip, and jaw control was examined by quantifying age-related changes in the similarity of each articulator’s movement patterns to those produced by adult subjects and by same-age peers. In addition, differences in token-to-token stability of articulatory movement were assessed among the different age groups. The experimental findings revealed that 1- and 2-year-old children’s jaw …

Osmosensitive Release Of Neurotransmitter Amino Acids: Relevance And Mechanisms, Herminia Pasantes-Morales, Rodrigo Franco, Lenin Ochoa, Benito Ordaz

School of Veterinary and Biomedical Sciences: Faculty Publications

Hyposmolarity activates amino acid efflux as part of the corrective volume process in a variety of cells. This review discusses the mechanism of amino acid release in brain cells preparations. Results present evidence of substantial differences between the efflux of taurine and that of GABA and glu-tamate, which besides a possible role as osmolytes, have a main function as synaptic transmitters. The differences found concern the efflux time course, the sensitivity to Cl^– channel blockers, the modulation by tyrosine kinases, the influence of PKC and the effect of cytoskeleton disruptive agents. While taurine efflux features fit well with the …

Wallinia Chavarriae N. Sp. (Trematoda: Macroderoididae) In Astyanax Aeneus (Günther, 1860) And Bryconamericus Scleroparius (Regan, 1908) (Osteichthyes: Characidae) From The Área De Conservación Guanacaste, Costa Rica, Anindo Choudhury, Rita Hartvigsen Daverdin, Daniel R. Brooks

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Wallinia chavarriae n. sp. is described from the small-bodied characids Astyanax aeneus and Bryconamericus scleroparius in the Área de Conservación Guanacaste, northwestern Costa Rica. The species differs from W. valenciae in possessing an acetabulum that is smaller than the oral sucker and vitelline follicles that are ovoid or rounded rather than elongate and tubular. Detailed comparison between these two species is handicapped by the less than satisfactory condition of the type and only museum specimen of W. valenciae. Wallinia chavarriae and W. valenciae belong to a subfamily of trematodes, Walliniinae, that arguably includes Creptotrematina spp., Magnivitellum simplex, and …

“The Policy Exists But You Can’T Really Use It”: Communication And The Structuration Of Work-Family Policies, Erika L. Kirby, Kathleen J. Krone

Department of Communication Studies: Faculty Publications

Although work-family benefits are increasingly important organizational policies, limited research addresses the impact of communication on benefit utilization. However, communication is significant because the perceived appropriateness of work-family benefits emerges through interaction. For example, when coworkers complain about “picking up the slack” for those using family leave, their discourse may impact future decisions of other workers regarding whether they utilize the work-family benefits available to them. We apply Giddens’ (1984) Structuration Theory to examine organizational members’ discursive responses to conditions (and contradictions) present in utilizing work-family benefits in a governmental organization. We argue the daily discursive practices of individuals can …

Lasers As Non-Lethal Avian Repellents: Potential Applications In The Airport Environment, Bradley F. Blackwell, Glen E. Bernhardt, Jonathon D. Cepek, Richard A. Dolbeer

United States Department of Agriculture Wildlife Services: Staff Publications

Research conducted by the U.S. Department of Agriculture’s (USDA) National Wildlife Research Center (NWRC) indicates that low- to moderate-power, long-wavelength lasers (630- 650 nm) provide an effective means of dispersing some “problem” bird species under low-light conditions, while presenting no threat to the animal or the environment. The NWRC researchers tested the hypothesis that birds would avoid the beam or beam spot produced by a laser in low ambient light. In the format of controlled, replicated, 2-choice experiments with captive birds, Canada geese (Branta canadensis; 6 groups; 4 birds/group) exhibited extreme avoidance of laser beams over 80-minute periods, …

Child Sexual Abuse, Eugenia Hsu, Georganna Sedlar, Mary Fran Flood, David J. Hansen

Department of Psychology: Faculty Publications

This chapter describes the case of two adolescent girls who were living in foster care with their maternal aunt and her family, the Kraller family. The girls and their aunt and uncle participated in Project SAFE, a university-based program for sexually abused children and their nonoffending caregivers, which is described in detail in the Course of Treatment section. The Kraller family was referred to Project SAFE by the local Child Advocacy Center. Miriam Kraller contacted the Child Advocacy Center when she learned that her two nieces (her younger sister’s daughters), Gina (age 14) and Suzy (age 13), had been sexually …

Suppressors Of Transcriptional Transgenic Silencing In Chlamydomonas Are Sensitive To Dna-Damaging Agents And Reactivate Transposable Elements, Byeong-Ryool Jeong, Dancia Wu-Scharf, Chaomei Zhang, Heriberto D. Cerutti

Center for Plant Science Innovation: Faculty Publications

In the unicellular green alga Chlamydomonas reinhardtii, the epigenetic silencing of transgenes occurs, as in land plants, at both the transcriptional and posttranscriptional levels. In the case of single-copy transgenes, transcriptional silencing takes place without detectable cytosine methylation of the introduced DNA. We have isolated two mutant strains, Mut-9 and Mut-11, that reactivate expression of a transcriptionally silenced single-copy transgene. These suppressors are deficient in the repression of a DNA transposon and a retrotransposon-like element. In addition, the mutants show enhanced sensitivity to DNA-damaging agents, particularly radiomimetic chemicals inducing DNA double- strand breaks. All of these phenotypes are much …

An Economic Analysis Of Predator Removal Approaches For Protecting Marine Turtle Nests At Hobe Sound National Wildlife Refuge, Richard M. Engeman, Stephanie A. Shwiff, Bernice Constantin, Margo Stahl, Henry T. Smith

United States Department of Agriculture Wildlife Services: Staff Publications

Hobe Sound National Wildlife Refuge (HSNWR) on Florida’s east coast provides undisturbed nesting habitat for three species of threatened or endangered marine turtles. Predation by raccoons and armadillos poses the greatest risk to turtle nests, and predator control has been identified as the most important management tool for enhancing nesting productivity. Recently, estimates of the number of nests that would have been lost in the 2000 nesting and incubation season were made using the results from four control approaches. These approaches were, in order of descending complexity: (1) refuge control enhanced by a one person-month contract with federal control specialists, …

Monetary Valuation Methods For Economic Analysis Of The Benefit-Costs Of Protecting Rare Wildlife Species From Predators, Richard M. Engeman, Stephanie A. Shwiff, Henry T. Smith, Bernice Constantin

United States Department of Agriculture Wildlife Services: Staff Publications

Many threatened and endangered species are negatively impacted by predation. Management of the predators that are geographically and temporally in position to threaten species of concern can greatly aid in their conservation. However, decisions on management strategies are based within economic constraints, while the success of management actions is measured by wildlife resource improvement. Here, we review methods for applying monetary valuations for threatened and endangered species so that economic analyses of management actions can be used to help guide and evaluate management decisions. A variety of applied examples are provided to demonstrate the principles.

Series Scarabaeiformia Crowson 1960, Superfamily Scarabaeoidea Latreille 1802, Mary Liz Jameson, Brett C. Ratcliffe

University of Nebraska State Museum: Entomology Papers

The superfamily Scarabaeoidea is a large, diverse, cosmopolitan group of beetles. Scarabaeoids are adapted to most habitats, and they are fungivores, herbivores, necrophages, coprophages, saprophages, and some are carnivores. They are widely distributed, even living in the Arctic in animal burrows. Some scarabs exhibit parental care and sociality. Some are myrmecophilous, termitophilous, or ectoparasitic. Many possess extravagant horns, others are able to roll into a compact ball, and still others are highly armored for inquiline life. Some are agricultural pests that may destroy crops while others are used in the biological control of dung and dung flies. Scarabaeoids are popular …

Yungasicola Travassosi Gen. N., Sp. N. (Digenea: Dicrocoeliidae: Eurytrematinae) From Two Species Of Grass Mice Of The Genus Akodon Meyen (Rodentia: Muridae) From The Yungas Of Bolivia, Scott Lyell Gardner, Gerardo Perez-Ponce De Leon

Harold W. Manter Laboratory of Parasitology: Faculty Publications

A new species and genus of digenetic trematode of the family Dicrocoeliidae is described from 2 species of grass mice, Akodon fumeus and Akodon mimus, both collected from an isolated area in the eastern part of the Andes mountains or Yungas of Bolivia. This species differs from all known dicrocoeliids in the unique disposition of the vitelline fields, the much less extensive uterus through the body of the worm (extending posteriad only just past the midline), and cecal branches that are generally conspicuous and lined with a thick layer of glandular epithelial tissue.