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Relationship Between Secondary Metabolism And Fungal Development, Ana M. Calvo, Richard A. Wilson, Jin Woo Bok, Nancy P. Keller Sep 2002

Relationship Between Secondary Metabolism And Fungal Development, Ana M. Calvo, Richard A. Wilson, Jin Woo Bok, Nancy P. Keller

Fungal Molecular Plant-Microbe Interactions

Details findings related to the relationship between secondary metabolism and fungal development.


Mycobacterium Smegmatis L-Alanine Dehydrogenase (Ald) Is Required For Proficient Utilization Of Alanine As A Sole Nitrogen Source And Sustained Anaerobic Growth, Zhengyu Feng, Nancy E. Caceres, Gautam Sarath, Raul G. Barletta Sep 2002

Mycobacterium Smegmatis L-Alanine Dehydrogenase (Ald) Is Required For Proficient Utilization Of Alanine As A Sole Nitrogen Source And Sustained Anaerobic Growth, Zhengyu Feng, Nancy E. Caceres, Gautam Sarath, Raul G. Barletta

School of Veterinary and Biomedical Sciences: Faculty Publications

NAD(H)-dependent L-alanine dehydrogenase (EC 1.4.1.1) (Ald) catalyzes the oxidative deamination of L-alanine and the reductive amination of pyruvate. To assess the physiological role of Ald in Mycobacterium smegmatis, we cloned the ald gene, identified its promoter, determined the protein expression levels, and analyzed the combined effects of nutrient supplementation, oxygen availability, and growth stage on enzyme activity. High Ald activities were observed in cells grown in the presence of L- or D-alanine regardless of the oxygen availability and growth stage. In exponentially growing cells under aerobic conditions, supplementation with alanine resulted in a 25- to 50-fold increase in the …


In Situ Corrosion Studies On The Battleship Uss Arizona, John D. Makinson, James D. Carr, Matthew A. Russell, David L. Conlin, Larry E. Murphy Sep 2002

In Situ Corrosion Studies On The Battleship Uss Arizona, John D. Makinson, James D. Carr, Matthew A. Russell, David L. Conlin, Larry E. Murphy

Department of Mechanical and Materials Engineering: Faculty Publications

U.S. National Park Service Submerged Resources Center archaeologists and University of Nebraska-Lincoln metallurgists are assessing hull corrosion by drilling through accumulated concretions and measuring pH and corrosion potentials. Concretion samples are being analyzed to determine the role of microbes in the corrosion process, identify chemical species, and measure electrical and physical properties. The lowest values of pH and E corr occur at the metal/concretion interface. Analysis suggests a variable corrosion rate supported by hydrogen discharge and/or oxygen reduction inside the concretion.


Use Of Main Or Branch Library Web Pages On Public Access Computers In Academic Branch Libraries: Results Of A Listserv Inquiry, Anita Breckbill, Virginia A. Baldwin Sep 2002

Use Of Main Or Branch Library Web Pages On Public Access Computers In Academic Branch Libraries: Results Of A Listserv Inquiry, Anita Breckbill, Virginia A. Baldwin

University of Nebraska-Lincoln Libraries: Faculty Publications

Some academic branch libraries display a main library Web page as the default home page for their public computers. Others display a branch library page. In response to an inquiry sent to selected listservs, librarians from sixty-three academic institutions provided information about the choice and content of their default home pages. Although the numbers from the study do not clearly favor either choice, this paper, in summarizing the librarians' input, provides insight into other available options and the considerations that contribute to maximum user benefit for a given library and library system.


Endangered And Threatened Wildlife And Plants: Designation Of Critical Habitat For The Topeka Shiner, Proposed Rule Aug 2002

Endangered And Threatened Wildlife And Plants: Designation Of Critical Habitat For The Topeka Shiner, Proposed Rule

Endangered Species Bulletin

We, the U.S. Fish and Wildlife Service, propose designation of critical habitat pursuant to the Endangered Species Act of 1973, as amended, for the Topeka shiner (Notropis topeka). This proposal is made in response to a court settlement in Biodiversity Legal Foundation et al. v. Ralph Morganweck et al. C00–D–1180, committing the Service to submit for publication in the Federal Register a proposal to withdraw the existing ‘‘not prudent’’ critical habitat determination together with a new proposed critical habitat determination for the Topeka shiner by August 13, 2002. We are proposing to designate as critical habitat a …


Nitrogen And Dry Matter Distribution By Culm And Leaf Position At Two Stages Of Vegetative Growth In Winter Wheat, Wally Wilhelm, G. S. Mcmaster, Dorothy M. Harrell Aug 2002

Nitrogen And Dry Matter Distribution By Culm And Leaf Position At Two Stages Of Vegetative Growth In Winter Wheat, Wally Wilhelm, G. S. Mcmaster, Dorothy M. Harrell

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Knowledge of N and assimilate partitioning in wheat (Triticum aestivum L.) improves management efficacy and crop model development. Our purpose was to describe N and dry matter distribution during vegetative growth of blades, sheaths, and internodes on four culms. Winter wheat grown at the Colorado State University Horticultural Farm was sampled at Haun Stage 5 and jointing. Samples were dried, weighed, and analyzed for N. As the canopy developed and older tissue contributed more of total tissue, N concentration decreased although N mass and dry weight increased. Dry matter and N mass decreased from MC to T1 and T2 …


Discovering Lactic Acid Bacteria By Genomics, Todd Klaenhammer, Eric Altermann, Fabrizio Arigoni, Alexander Bolotin, Fred Breidt, Jeffrey Broadbent, Raul Cano, Stephane Chaillou, Josef Deutscher, Mike Gasson, Maarten Van De Gutche, Jean Guzzo, Axel Hartke, Trevor Hawkins, Pascal Hols, Robert W. Hutkins, Michiel Kleerebezem, Jan Kok, Oscar Kuipers, Mark Lubbers, Emanuelle Maguin, Larry Mckay, David Mills, Arjen Nauta, Ross Overbeek, Herman Pel, David Pridmore, Milton Saier, Douwe Van Sinderen, Alexei Sorokin, James Steele, Daniel O'Sullivan, Willem De Vos, Bart Weimer, Monique Zagorec, Roland Seizen Aug 2002

Discovering Lactic Acid Bacteria By Genomics, Todd Klaenhammer, Eric Altermann, Fabrizio Arigoni, Alexander Bolotin, Fred Breidt, Jeffrey Broadbent, Raul Cano, Stephane Chaillou, Josef Deutscher, Mike Gasson, Maarten Van De Gutche, Jean Guzzo, Axel Hartke, Trevor Hawkins, Pascal Hols, Robert W. Hutkins, Michiel Kleerebezem, Jan Kok, Oscar Kuipers, Mark Lubbers, Emanuelle Maguin, Larry Mckay, David Mills, Arjen Nauta, Ross Overbeek, Herman Pel, David Pridmore, Milton Saier, Douwe Van Sinderen, Alexei Sorokin, James Steele, Daniel O'Sullivan, Willem De Vos, Bart Weimer, Monique Zagorec, Roland Seizen

Department of Food Science and Technology: Faculty Publications

This review summarizes a collection of lactic acid bacteria that are now undergoing genomic sequencing and analysis. Summaries are presented on twenty different species, with each overview discussing the organisms fundamental and practical significance, environmental habitat, and its role in fermentation, bioprocessing, or probiotics. For those projects where genome sequence data were available by March 2002, summaries include a listing of key statistics and interesting genomic features. These efforts will revolutionize our molecular view of Gram-positive bacteria, as up to 15 genomes from the low GC content lactic acid bacteria are expected to be available in the public domain by …


Cropwatch No. 2002-19, Aug. 9, 2002, Lisa Brown Jasa Aug 2002

Cropwatch No. 2002-19, Aug. 9, 2002, Lisa Brown Jasa

Crop Watch (1994–2005)

Inside

Field updates ......................... 174

Corn Growers Tour ................. 174

Nitrates and prussic acid ....... 176

Cowpea aphid .................. ...... 177

Planting alfalfa ....................... 178

Blister beetles ......................... 178

Irrigation field tours .............. 179

Nutrient research tour ............ 179

Agronomy classes ................. 180

Preventing wheat diseases .... ......................... 180

Forage poisoning misconceptions .................. 180


Hexapod Herald - Vol. 14, No. 4, [August?] 2002 Aug 2002

Hexapod Herald - Vol. 14, No. 4, [August?] 2002

Hexapod Herald and Other Entomology Department Newsletters

Graduate student news

From the office

Grants

Publications

Travel

This 'n that


United States Patent Application Publication: Isolation And Characterization Of Heat Shock Protein, Thomas Elthon, Adrian A. Lund, Dinakar Bhattramakki, David Rhoads Aug 2002

United States Patent Application Publication: Isolation And Characterization Of Heat Shock Protein, Thomas Elthon, Adrian A. Lund, Dinakar Bhattramakki, David Rhoads

Center for Plant Science Innovation: Faculty Publications

This invention relates to the identification and characterization of the maize HSP22 protein including the corresponding nucleic acid molecules, nucleic acid fragments and protein fragments. Further, this invention relates to both polyclonal and monoclonal antibodies to HSP22 and the production thereof. Those antibodies can be used in a method for detecting the presence of HSP22 expression in whole leaf samples. Further, the nucleic acid molecule and fragments thereof can be used in assays to detect the levels of nucleic acid molecules involved in HSP22 expression. The levels of both HSP22 and HSP22 mRNA are then utilized as an indication of …


University Of Nebraska–Lincoln Extension Connect, August 2002 Aug 2002

University Of Nebraska–Lincoln Extension Connect, August 2002

Connect (University of Nebraska-Lincoln Extension)

Inside this issue:

pg. 3 Environmental education
pg. 4 Grape-growing ventures
pg. 7 Plant Disease Central


Ard News August 2002 Aug 2002

Ard News August 2002

Agricultural Research Division: News and Annual Reports

CONTENTS:

Comments from the Dean
ARD Unit Resources and Performance Indicators for FY 2001
Status of FY 2003 CSREES Appropriations
Nebraska Research Initiative Grants
Research Authorizations in the 2002 Farm Bill
Undergraduate Honors Research Program
New or Revised Projects
Proposals Submitted for Federal Grants
Grants and Contracts Received June and July, 2002
Hardin Distinguished Graduate Fellowship for 2002-2003
Widaman Trust Distinguished Graduate Assistant Award


Sodium Dodecyl Sulfate Hypersensitivity Of Clpp And Clpb Mutants Of Escherichia Coli, Soumitra Rajagopal, Narasimhan Sudarsan, Kenneth W. Nickerson Aug 2002

Sodium Dodecyl Sulfate Hypersensitivity Of Clpp And Clpb Mutants Of Escherichia Coli, Soumitra Rajagopal, Narasimhan Sudarsan, Kenneth W. Nickerson

Papers in Microbiology

We studied the hypersensitivity of clpP and clpB mutants of Escherichia coli to sodium dodecyl sulfate (SDS). Both wild-type E. coli MC4100 and lon mutants grew in the presence of 10% SDS, whereas isogenic clpP and clpB single mutants could not grow above 0.5% SDS and clpA and clpX single mutants could not grow above 5.0% SDS. For wild-type E. coli, cellular ClpP levels as determined by Western immunoblot analysis increased ca. sixfold as the levels of added SDS increased from 0 to 2%. Capsular colanic acid, measured as uronic acid, increased ca. sixfold as the levels of added …


Testing For Genetic Evidence Of Population Expansion And Contraction: An Empirical Analysis Of Microsatellite Dna Variation Using A Hierarchical Bayesian Model, Jay F. Storz, Mark Beaumont Aug 2002

Testing For Genetic Evidence Of Population Expansion And Contraction: An Empirical Analysis Of Microsatellite Dna Variation Using A Hierarchical Bayesian Model, Jay F. Storz, Mark Beaumont

Jay F. Storz Publications

The role of past climatic change in shaping the distributions of tropical rain forest vertebrates is central to long-standing hypotheses about the legacy of the Quaternary ice ages. One approach to testing such hypotheses is to use genetic data to infer the demographic history of codistributed species. Population genetic theory that relates the structure of allelic genealogies to historical changes in effective population size can be used to detect a past history of demographic expansion or contraction. The fruit bats Cynopterus sphinx and C. brachyotis (Chiroptera: Pteropodidae) exhibit markedly different distribution patterns across the Indomalayan region and therefore represent an …


Extended Visions July-August 2002 Aug 2002

Extended Visions July-August 2002

Extended Visions Newsletter (ARDC)

No abstract provided.


Nebline, August 2002 Aug 2002

Nebline, August 2002

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

4-H School Enrichment in Action!
NEP/4-H School Enrichment Participants Eat Healthier
A Community of Character!
Perennials with Colorful Foliage
An Easy Way to Dry Flowers
All America Rose Selections, All Time Classics
2002 August/September Garden Calendar
Stinging Wasps and Bees
Water Conservation Tips
Pesticides in Schools
Bat Sightings Increase in August
Is Fall or Spring Seeding of Alfalfa Best?
Farm Bill Informational Sessions Scheduled
Predicting the Last Irrigation
Deadline Approaches to Terminate Oral Farm Leases
Designing a Low Maintenance Landscape
Free Disease Testing for Backyard Poultry Flocks
New Pesticide Disposal Instructions
NU Landscape Connections: A Showcase Event
Other August/September UNL …


Water Current, Volume 34, No. 4. August 2002 Aug 2002

Water Current, Volume 34, No. 4. August 2002

Water Current Newsletter

• Summer Tour Attracts More Than 80 by Steve Ress

• From the Director

• Meet the Faculty

• We All Live Within One System by Mary Harding

• Has the Time Come To Look at Urban Stormwater Differently? By Glenn D. Johnson

• Mapping Groundwater Recharge for Nebraska

• Nebraska Water Marketing Policy Choices by J. David Aiken

• Water News Briefs

• A Union of Festibal of Color and Gardener’s Gala by Steve Ress


A Regional Gis-Based Analysis Of Elk Habitat Suitability In Northeast Nebraska, J W. Fisher Aug 2002

A Regional Gis-Based Analysis Of Elk Habitat Suitability In Northeast Nebraska, J W. Fisher

School of Natural Resources: Dissertations, Theses, and Student Research

No abstract provided.


A Regional Gis-Based Analysis Of Elk Habitat Suitability In Northwestern Nebraska, Justin W. Fischer Aug 2002

A Regional Gis-Based Analysis Of Elk Habitat Suitability In Northwestern Nebraska, Justin W. Fischer

School of Natural Resources: Dissertations, Theses, and Student Research

The need to accurately assess the use and suitability of elk (Cervus elaphus spp.) habitat at regional scales will continue to increase as human development encroaches into what was once optimum elk habitat. The objectives of this research were to calculate the relative proportions of habitat use by elk in the Pine Ridge region of Northwestern Nebraska from a set of radio-collared elk locations and generate a habitat suitability model. Habitat variables (Iandcover type, aspect, slope, distance to a road, road density, and distance from the ponderosa pine edge) were measured for each elk location (n = 5,787 dating …


Influence Of Light And Nutrients On Atrazine Toxicity To Freshwater Algae, Crystal J. Hansen Aug 2002

Influence Of Light And Nutrients On Atrazine Toxicity To Freshwater Algae, Crystal J. Hansen

School of Natural Resources: Dissertations, Theses, and Student Research

No abstract provided.


Re-Test Of Rhinocyllus Conicus Host Specificity, And The Prediction Of Ecological Risk In Biological Control, Amy E. Arnett, Svata M. Louda Aug 2002

Re-Test Of Rhinocyllus Conicus Host Specificity, And The Prediction Of Ecological Risk In Biological Control, Amy E. Arnett, Svata M. Louda

School of Biological Sciences: Faculty Publications

Biological control is proposed as an ecological strategy to manage the threat of invasive plants, especially in natural areas. To pursue this strategy, we need to know that the host specificity criteria used to evaluate ecological risk with deliberate introduction of an exotic insect for biocontrol are sufficient to predict potential impact on native species. Host specificity is defined by adult feeding and oviposition preferences and larval development. One way to evaluate the criteria is to re-examine case histories where ecological effects are recorded, such as that of Rhinocyllus conicus Frölich. This flower head weevil, released in North America in …


Dietary Overlap Between Wolves And Coyotes In Northwestern Montana, Wendy M. Arjo, Daniel H. Pletscher, Robert R. Ream Aug 2002

Dietary Overlap Between Wolves And Coyotes In Northwestern Montana, Wendy M. Arjo, Daniel H. Pletscher, Robert R. Ream

United States Department of Agriculture Wildlife Services: Staff Publications

We studied effects of recolonizing wolves (Canis lupus) in the North Fork of the Flathead area of northwestern Montana on the diets of coyotes (C. latrans) from 1994 to 1997. Wolf and coyote diets differed in frequency of occurrence of prey species during 3 of the 4 summers and winters (P< 0.001) during the study. Coyote diets contained more murid prey items, and wolf diets contained more deer (Odocoileus virginianus and O. hemionus) in the summer and elk (Cervus elaphus) in the winter. Coyotes and wolves ate prey of different size during both the summer (P < 0.001) and winter (P < 0.001) months in 1994– 1996: wolves took a greater proportion (P < 0.001) of large (>45 kg) prey species and coyotes, small (<2 kg) prey (P < 0.001). Wolves selected a larger proportion of adults (P …


Use Of Sensory Cues By Fish Crows Corvus Ossifragus Preying On Artificial Bird Nests, Leonard Santisteban, Kathryn E. Sieving, Michael L. Avery Jul 2002

Use Of Sensory Cues By Fish Crows Corvus Ossifragus Preying On Artificial Bird Nests, Leonard Santisteban, Kathryn E. Sieving, Michael L. Avery

United States Department of Agriculture Wildlife Services: Staff Publications

How predators locate avian nests is poorly understood and has been subjected to little experimental inquiry. We examined which sensory stimuli were important in the nest-finding behavior of fish crowsCorvus ossifragus, a common nest predator in the southeastern United States. Using an array of potted trees in a large enclosure, we presented artificial nests to captive crows and quantified responses to visual, auditory, and olfactory nest cues, and nest position. Partial ranks of nest-treatment preferences were analyzed using log-linear models. Nest visibility significantly increased the likelihood of predation by fish crows and increasing nest height was a marginally significant influence …


Survey Of Hydrogen Sulfide Concentrations In Vicinity Of Beef Cattle Feedlots, Richard K. Koelsch, Bryan L. Woodbury, David E. Stenberg, Daniel N. Miller, Dennis D. Schulte Jul 2002

Survey Of Hydrogen Sulfide Concentrations In Vicinity Of Beef Cattle Feedlots, Richard K. Koelsch, Bryan L. Woodbury, David E. Stenberg, Daniel N. Miller, Dennis D. Schulte

Department of Agricultural and Biological Systems Engineering: Presentations and White Papers

A field survey of Total Reduced Sulfur (TRS) concentrations in the vicinity of beef cattle feedlots was conducted to compare field observations against current regulatory thresholds. In addition, environmental factors that may contribute to increased TRS emissions were evaluated. It was observed that TRS levels in the vicinity of beef cattle feedlots are not likely to exceed current regulatory thresholds used by mid-western states. It was further noted that concentration of TRS varies with air temperature and time of day. However, wet feedlot surface conditions and wind speed had almost no impact upon observed concentrations.


Cropwatch No. 2002-18, July 26, 2002, Lisa Brown Jasa Jul 2002

Cropwatch No. 2002-18, July 26, 2002, Lisa Brown Jasa

Crop Watch (1994–2005)

Inside

Management tips……….164

Field updates……….……….164

Soybean aphid research……….……….165

Yellow soybeans……….……….167

Clearfield wheat system……….……….168

Volunteer wheat……….……….168

SCREC field day……….……….169

2nd ECB flight……….……….170

Management worksheet……….……….170

Greenbugs in sorghum……….……….171

Forage from soybeans……….……….172

Drought Web page……….……….172


Eicosanoids Mediate Manduca Sexta Cellular Response To The Fungal Pathogen Beauveria Bassiana: A Role For The Lipoxygenase Pathway, Jeffrey C. Lord, Sheri Anderson, David W. Stanley Jul 2002

Eicosanoids Mediate Manduca Sexta Cellular Response To The Fungal Pathogen Beauveria Bassiana: A Role For The Lipoxygenase Pathway, Jeffrey C. Lord, Sheri Anderson, David W. Stanley

Department of Entomology: Faculty Publications

Many studies have documented the involvement of eicosanoids in insect cellular immune responses to bacteria. The use of the fungal pathogen Beauveria bassiana as a nodulation elicitor, with inhibition of phospholipase A2 by dexamethasone, extends the principle to fungi. This study also provides the first evidence of involvement of the lipoxygenase (LOX) pathway rather than the cyclooxygenase (COX) pathway in synthesis of the nodulation mediating eicosanoid(s). The LOX product, 5(S)- hydroperoxyeicosa-6E,8Z,11Z,14Z-tetraenoic acid (5-HPETE), substantially reversed nodulation inhibition caused by dexamethasone and the LOX inhibitors, caffeic acid and esculetin. The COX product, prostaglandin H2 (PGH2), did not reverse …


Waterfowl Population Status, 2002, Pamela R. Garrettson, Timothy J. Moser, Khristi Wilkins Jul 2002

Waterfowl Population Status, 2002, Pamela R. Garrettson, Timothy J. Moser, Khristi Wilkins

United States Fish and Wildlife Service: Publications

In the traditional survey area (strata 1-18, 20-50, and 75-77), total duck abundance was 31.2 + 0.5 [SE] million birds. This was 14% below (P< 0.001) last year’s estimate of 36.1 ± 0.6 million birds, and 6% below the long-term (1955-2001) average (P<0.001). Mallard (Anas platyrhynchos) abundance was 7.5 ± 0.2 million, similar to (P=0.23) the 2001 estimate of 7.9 ± 0.2 million, and essentially identical to the long-term average (P=1.00). Blue-winged teal (A. discors) abundance was 4.2 ± 0.2 million, which was 27% below last year’s estimate of 5.8 ± 0.3 million (P<0.001), but similar to the long-term average (P=0.22). Gadwall (A. strepera; 2.2 ± 0.1 million, -17%), shovelers (A. clypeata; 2.3 ± 0.1 million, -30%), …


Cropwatch No. 2002-17, July 19, 2002, Lisa Brown Jasa Jul 2002

Cropwatch No. 2002-17, July 19, 2002, Lisa Brown Jasa

Crop Watch (1994–2005)

Inside

Management tips……….158

Field updates……….……….158

Correction……….……….158

Drought widens……….……….160

Corn rootworm injury……….……….161

Injury scale diagram……….……….161

Drought meetings……….……….162

Wheat market rally……….……….162

See Ag News and more stories on the Web at http://cropwatch.unl.edu


Beaver Herbivory And Its Effect On Cottonwood Trees: Influence Of Flooding Along Matched Regulated And Unregulated Rivers, Stewart W. Breck, Kenneth R. Wilson, Douglas C. Andersen Jul 2002

Beaver Herbivory And Its Effect On Cottonwood Trees: Influence Of Flooding Along Matched Regulated And Unregulated Rivers, Stewart W. Breck, Kenneth R. Wilson, Douglas C. Andersen

United States Department of Agriculture Wildlife Services: Staff Publications

We compared beaver (Castor canadensis) foraging patterns on Fremont cottonwood (Populus deltoides subsp. wislizenii ) saplings and the probability of saplings being cut on a 10 km reach of the flow-regulated Green River and a 8.6 km reach of the free-flowing Yampa River in northwestern Colorado. We measured the abundance and density of cottonwood on each reach and followed the fates of individually marked saplings in three patches of cottonwood on the Yampa River and two patches on the Green River. Two natural floods on the Yampa River and one controlled flood on the Green River between …


Genetic Evidence For Long-Term Population Decline In A Savannah Dwelling Primate: Inferences From A Hierarchical Bayesian Model, Jay F. Storz, Mark A. Beaumont, Susan C. Alberts Jul 2002

Genetic Evidence For Long-Term Population Decline In A Savannah Dwelling Primate: Inferences From A Hierarchical Bayesian Model, Jay F. Storz, Mark A. Beaumont, Susan C. Alberts

Jay F. Storz Publications

The purpose of this study was to test for evidence that savannah baboons (Papio cynocephalus) underwent a population expansion in concert with a hypothesized expansion of African human and chimpanzee populations during the late Pleistocene. The rationale is that any type of environmental event sufficient to cause simultaneous population expansions in African humans and chimpanzees would also be expected to affect other codistributed mammals. To test for genetic evidence of population expansion or contraction, we performed a coalescent analysis of multilocus microsatellite data using a hierarchical Bayesian model. Markov chain Monte Carlo (MCMC) simulations were used to estimate …