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Acute Toxicity Of Atrazine, Alachlor, And Chlorpyrifos Mixtures To Honey Bees, Christopher J. Fellows, Troy D. Anderson, Daniel R. Swale Nov 2022

Acute Toxicity Of Atrazine, Alachlor, And Chlorpyrifos Mixtures To Honey Bees, Christopher J. Fellows, Troy D. Anderson, Daniel R. Swale

Department of Entomology: Faculty Publications

The acute toxicity of chlorpyrifos and chlorpyrifos-oxon (organophosphorothioate insecticides) was examined alone and in combination with atrazine (triazine herbicide) and alachlor (chloroacetanilide herbicide) to honey bees (Apis mellifera). Atrazine and alachlor were observed to not be acutely toxic to bees at doses up to 10 and 4 μg per bee, respectively. However, atrazine significantly increased chlorpyrifos toxicity by 3-fold while reducing chlorpyrifos-oxon toxicity by 1.8-fold. These changes in toxicity are correlated with significant 1.3- and 1.2-fold inhibition of acetylcholinesterase (AChE) activity in bees exposed to chlorpyrifos and chlorpyrifos-oxon, respectively. Atrazine significantly increased cytochrome P450, general esterase, and glutathione …


A Method Of Assessing Peripheral Stent Abrasiveness Under Cyclic Deformations Experienced During Limb Movement, Courtney Keiser, Kaspars Maleckis, Pauline Struczewska, Majid Jadidi, Jason N. Mactaggart, Alexey Kamenskiy Nov 2022

A Method Of Assessing Peripheral Stent Abrasiveness Under Cyclic Deformations Experienced During Limb Movement, Courtney Keiser, Kaspars Maleckis, Pauline Struczewska, Majid Jadidi, Jason N. Mactaggart, Alexey Kamenskiy

Department of Mechanical and Materials Engineering: Faculty Publications

Poor outcomes of peripheral arterial disease stenting are often attributed to the inability of stents to accommodate the complex biomechanics of the flexed lower limb. Abrasion damage caused by rubbing of the stent against the artery wall during limb movement plays a significant role in reconstruction failure but has not been characterized. Our goals were to develop a method of assessing the abrasiveness of peripheral nitinol stents and apply it to several commercial devices. Misago, AbsolutePro, Innova, Zilver, SmartControl, SmartFlex, and Supera stents were deployed inside electrospun nanofibrillar tubes with femoropopliteal artery-mimicking mechanical properties and subjected to cyclic axial compression …


Identifying Early-Life Behavior To Predict Mothering Ability In Swine Utilizing NuTrack System, Savannah Gabrielle Millburn Nov 2022

Identifying Early-Life Behavior To Predict Mothering Ability In Swine Utilizing NuTrack System, Savannah Gabrielle Millburn

Department of Animal Science: Dissertations, Theses, and Student Research

Early recognition of indicator traits for swine reproduction and longevity supports economical selection decision making. Gilt activity is a key variable impacting a sow’s herd life and productivity. The purpose of this study was to examine early- life behaviors contributing to farrowing traits including gestation length (GL), number born alive (NBA), number weaned (NW), and herd life (HL). Herd life was a binary trait representing if a gilt was culled after one parity. Beginning at approximately 20 weeks of age, video recordings were taken on 480 gilts for 7 consecutive days and processed using the NUtrack system. Activity traits …


The Effects Of Surface Composition On 6-Weeks Of Plyometric Training, Cameron D. Addie, Richard S. Farley, Sandra L. Stevens, Halle J. Brandt3, Jocelyn E. Arnett, Jennifer L. Caputo Oct 2022

The Effects Of Surface Composition On 6-Weeks Of Plyometric Training, Cameron D. Addie, Richard S. Farley, Sandra L. Stevens, Halle J. Brandt3, Jocelyn E. Arnett, Jennifer L. Caputo

Department of Nutrition and Health Sciences: Faculty Publications

Background: Plyometric training programs may be performed on a hard surface or a soft surface to target specific training adaptations and enhance jump performance. However, it is unknown how surface compliance impacts jump performance. Objective: To compare changes in horizontal lower body power following a 6-week plyometric training program performed on a soft surface (n = 9) and a hard surface (n = 11). Methods: This was a quasi-experimental study. University students (N = 20; males = 11, females = 9; age: 20.4 ± 3.7 yr; body mass: 68.4 ± 12.5 kg; height 1.7 …


Environmental Influence On Stickleback Fish Phenotype, Alaina A. Young Oct 2022

Environmental Influence On Stickleback Fish Phenotype, Alaina A. Young

RURALS: Review of Undergraduate Research in Agricultural and Life Sciences

Abstract

Sticklebacks have grown to be a model organism in the last few decades because they have undergone a rapid adaptive radiation event. Portions of the original marine population have migrated to isolated freshwater ecosystems and their fundamental niche has expanded. Variation among select phenotypic traits is a direct result of selective pressures imposed by new environment types. Lateral plates have been the most widely analyzed trait, it is hypothesized that great variation in plate quantity has resulted from expansion events. Lateral plates are pieces of an exoskeleton in addition to the already established endoskeleton. This study aims to assess …


Near-Atomic, Non-Icosahedrally Averaged Structure Of Giant Virus Paramecium Bursaria Chlorella Virus 1, Qianqian Shao, Irina V. Agarkova, Eric A. Noel, David D. Dunigan, Yunshu Liu, Aohan Wang, Mingcheng Guo, Linlin Xie, Xinyue Zhao, Michael G. Rossmann, James L. Van Etten, Thomas Klose, Qianglin Fang Oct 2022

Near-Atomic, Non-Icosahedrally Averaged Structure Of Giant Virus Paramecium Bursaria Chlorella Virus 1, Qianqian Shao, Irina V. Agarkova, Eric A. Noel, David D. Dunigan, Yunshu Liu, Aohan Wang, Mingcheng Guo, Linlin Xie, Xinyue Zhao, Michael G. Rossmann, James L. Van Etten, Thomas Klose, Qianglin Fang

Department of Plant Pathology: Faculty Publications

Giant viruses are a large group of viruses that infect many eukaryotes. Although components that do not obey the overall icosahedral symmetry of their capsids have been observed and found to play critical roles in the viral life cycles, identities and high-resolution structures of these components remain unknown. Here, by determining a near-atomic-resolution, five-fold averaged structure of Parameciumbursaria chlorella virus 1, we unexpectedly found the viral capsid possesses up to five major capsid protein variants and a penton protein variant. These variants create varied capsidmicroenvironments for the associations of fibers, a vesicle, and previously unresolved minor capsid proteins. Our structure …


Host Selection And Nesting Behavior Of Nearctic Trapdoor Spider-Hunting Spider Wasps (Hymenoptera: Pompilidae: Pepsinae, Pompilinae), Frank E. Kurczewski, Rick C. West Oct 2022

Host Selection And Nesting Behavior Of Nearctic Trapdoor Spider-Hunting Spider Wasps (Hymenoptera: Pompilidae: Pepsinae, Pompilinae), Frank E. Kurczewski, Rick C. West

Insecta Mundi

Host records and nesting behavior of the Nearctic trapdoor spider-hunting spider wasps (Hy­menoptera: Pompilidae) Calopompilus Ashmead and Priocnemissus Haupt (Pepsinae: Pepsini) and Aporus Spinola and Psorthaspis Banks (Pompilinae: Aporini) are reviewed, investigated, compared, and discussed. First time incidental trapdoor spider host records for Priocnemis (Priocnemissus) minorata Banks (Pepsi­nae: Pepsini), Anoplius (Lophopompilus) carolina (Banks) (Pompilinae: Pompilini), and Notocyphus dorsalis dorsalis Cresson (Notocyphinae: Notocyphini) are included, although they are not typical trapdoor spider-hunting spider wasp species. The Palearctic Aporus (Aporus) unicolor Spinola, A. (Aporus) bicolor Spinola and A. (Aporus) planiceps (Latreille) are …


First Host Record, Nesting Behavior, And Taxonomic Position Of The Spider Wasp Genus Hesperopompilus Evans And Some Other Evans Genera (Hymenoptera: Pompilidae), Frank E. Kurczewski, Rick C. West, James P. Pitts Oct 2022

First Host Record, Nesting Behavior, And Taxonomic Position Of The Spider Wasp Genus Hesperopompilus Evans And Some Other Evans Genera (Hymenoptera: Pompilidae), Frank E. Kurczewski, Rick C. West, James P. Pitts

Insecta Mundi

First host record, prey transport, and burrow excavation are described for Hesperopompilus sp., an undescribed, rare spider wasp (Hymenoptera: Pompilidae) from Texas. Taxonomic, ecological, and behav­ioral examination of the genus subsequently led to an investigation of the previously related Perissopompilus Evans and Xerochares Evans. Taxonomic, host preference, nesting behavior, and phylogenomic relationships of the three taxa are discussed along with those of Xenopompilus Evans. The molecular connection of Perisso­pompilus and Allochares Banks is supported by their common use of host species of Filistatidae.

Evans (1951), in his taxonomic study of the spider wasp tribe Pompilini (Hymenoptera: Pompilidae: Pompilinae), described the …


Synopsis Of The Subfamily Carventinae In New Zealand (Heteroptera: Aradidae), Marie-Claude Larivière, André Larochelle Oct 2022

Synopsis Of The Subfamily Carventinae In New Zealand (Heteroptera: Aradidae), Marie-Claude Larivière, André Larochelle

Insecta Mundi

The subfamily Carventinae (Heteroptera: Aradidae) is revised for New Zealand. Eight genera and fifteen species are recognized.

One genus and six species are described as new: Carventaptera hallae Larivière and Larochelle new species, Lissaptera heissi Larivière and Larochelle new species, Modicarventus kirmani Larivière and Larochelle new species, Neocarventus montanus Larivière and Larochelle new species, Neocarventus northlandicus Larivière and Larochelle new species, Neocarventus potterae Larivière and Larochelle new species, Tuataraptera Larivière and Larochelle new genus.

One new combination is established: Neocarventus uncus Kirman, 1989 = Tuataraptera unca (Kirman, 1989).

One new synonymy is made: …


The 3p Framework: A Comprehensive Approach To Coping With The Emerging Infectious Disease Crisis, Orsolya Molnár, Eric Hoberg, Valeria Trivellone, Gábor Földvári, Daniel R. Brooks Oct 2022

The 3p Framework: A Comprehensive Approach To Coping With The Emerging Infectious Disease Crisis, Orsolya Molnár, Eric Hoberg, Valeria Trivellone, Gábor Földvári, Daniel R. Brooks

MANTER: Journal of Parasite Biodiversity

The COVID-19 pandemic is the latest example of the profound socioeconomic impact of the emerging infectious disease (EID) crisis. Current health security measures are based on a failed evolutionary paradigm that presumes EID is rare and cannot be predicted because emergence requires the prior evolution of novel genetic capacities for colonizing a new host. Consequently, crisis response through preparation for previously emerged diseases and palliation following outbreaks have been the only health security options, which have become unsustainably expensive and unsuccessful. The Stockholm paradigm (SP) is an alternative evolutionary framework that suggests host changes are the result of changing conditions …


Dynamics Of Crop Evapotranspiration Of Four Major Crops On A Large Commercial Farm: Case Of The Navajo Agricultural Products Industry, New Mexico, Usa, Koffi Djaman, Komlan Koudahe, Ali T. Mohammed Oct 2022

Dynamics Of Crop Evapotranspiration Of Four Major Crops On A Large Commercial Farm: Case Of The Navajo Agricultural Products Industry, New Mexico, Usa, Koffi Djaman, Komlan Koudahe, Ali T. Mohammed

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Crop evapotranspiration (ETa) is the main source of water loss in farms and watersheds, and with its effects felt at a regional scale, it calls for irrigation professionals and water resource managers to accurately assess water requirements to meet crop water use. On a multi-crop commercial farm, different factors affect cropland allocation, among which crop evapotranspiration is one of the most important factors regarding the seasonally or annually available water resources for irrigation in combination with the in-season effective precipitation. The objective of the present study was to estimate crop evapotranspiration for four major crops grown on the Navajo Agricultural …


The Effect Of Arbuscular Mycorrhizae On Milkweed Growth And Its Implications For Western Monarchs, Rylee Mattson, Estrella Silva, Katherine Klassen, Laurie Dizney Oct 2022

The Effect Of Arbuscular Mycorrhizae On Milkweed Growth And Its Implications For Western Monarchs, Rylee Mattson, Estrella Silva, Katherine Klassen, Laurie Dizney

RURALS: Review of Undergraduate Research in Agricultural and Life Sciences

Western monarch butterfly (Danaus plexippus plexippus) populations in the Pacific Northwest have decreased by approximately 99% in recent decades due in part to the destruction of milkweed (Asclepias spp.) habitat. Milkweeds are one of the only plant genera upon which monarchs will lay eggs and the larvae will feed, so milkweed habitat restoration is critical for the future survival and rehabilitation of western monarch butterfly populations. This research aimed to investigate the effect of the root symbiont arbuscular mycorrhizal fungus (AMF) on growth for two species of milkweed native to Oregon’s Willamette Valley. In showy milkweed …


Electrophilic Aldehyde 4-Hydroxy-2-Nonenal Mediated Signaling And Mitochondrial Dysfunction, Sudha Sharma, Papori Sharma, Tara Bailey, Susmita Bhattarai, Utsab Subedi, Chloe Miller, Hosne Ara, Srivatsan Kidambi, Hong Sun, Manikandan Panchatcharam, Sumitra Miriyala Oct 2022

Electrophilic Aldehyde 4-Hydroxy-2-Nonenal Mediated Signaling And Mitochondrial Dysfunction, Sudha Sharma, Papori Sharma, Tara Bailey, Susmita Bhattarai, Utsab Subedi, Chloe Miller, Hosne Ara, Srivatsan Kidambi, Hong Sun, Manikandan Panchatcharam, Sumitra Miriyala

Department of Chemical and Biomolecular Engineering: Faculty Publications

Reactive oxygen species (ROS), a by-product of aerobic life, are highly reactive molecules with unpaired electrons. The excess of ROS leads to oxidative stress, instigating the peroxidation of polyunsaturated fatty acids (PUFA) in the lipid membrane through a free radical chain reaction and the formation of the most bioactive aldehyde, known as 4-hydroxynonenal (4-HNE). 4-HNE functions as a signaling molecule and toxic product and acts mainly by forming covalent adducts with nucleophilic functional groups in proteins, nucleic acids, and lipids. The mitochondria have been implicated as a site for 4-HNE generation and adduction. Several studies clarified how 4-HNE affects the …


Multi‐Scale Effects Of Land Cover, Weather, And Fire On Columbian Sharp‐Tailed Grouse, Bryan S. Stevens, Courtney J. Conway, Jeffrey M. Knetter, Shane B. Roberts, Patrick Donnelly Oct 2022

Multi‐Scale Effects Of Land Cover, Weather, And Fire On Columbian Sharp‐Tailed Grouse, Bryan S. Stevens, Courtney J. Conway, Jeffrey M. Knetter, Shane B. Roberts, Patrick Donnelly

United States Fish and Wildlife Service: Publications

Columbian sharp‐tailed grouse (Tympanuchus phasianellus columbianus) are endemic to grassland and shrub‐steppe ecosystems of western North America, yet their distribution has contracted to <10% of their historical range. Primary threats to Columbian sharp‐tailed grouse include loss of native habitat and conversion to agriculture, reductions in habitat once provided by the Conservation Reserve Program (CRP), wildfire, and drought conditions, yet population‐level consequences of these threats and their spatio‐temporal scales of effect are poorly understood. We evaluated multi‐scale effects of land cover, weather, and fire histories on patterns of abundance and productivity for Columbian sharp‐tailed grouse populations during 1995–2020 in Idaho, USA, using mixedeffects generalized regression and remotely sensed data. We demonstrated negative effects of fire, tree encroachment, and bare ground, positive effects of spring and summer precipitation and cover of shrubs and perennial forbs and grasses, and positive effects of CRP on grouse abundance that changed in magnitude with cover of perennials and shrubs near leks (i.e., strongest effects when average cover of shrubs and perennial forbs and grasses were less abundant). We also demonstrated per capita recruitment of Columbian sharp‐tailed grouse is positively associated with late‐summer greenness. Our results show that several suspected threats have measurable, population‐level impacts to Columbian sharp‐tailed grouse within Idaho. Moreover, our results suggest ongoing changes occurring within the core range of Columbian sharp‐tailed grouse, including loss of CRP cover to tilled agriculture and changes to wildfire and precipitation dynamics are likely to have negative effects on populations.


Function Of Bidirectional Sensitivity In The Otolith Organs Established By Transcription Factor Emx2, Young Rae Ji, Yosuke Tona, Talah Wafa, Matthew E. Christman, Edward D. Tourney, Tao Jiang, Sho Ohta, Hui Cheng, Tracy Fitzgerald, Bernd Fritzsch, Sherri M. Jones, Kathleen E. Cullen, Doris K. Wu Oct 2022

Function Of Bidirectional Sensitivity In The Otolith Organs Established By Transcription Factor Emx2, Young Rae Ji, Yosuke Tona, Talah Wafa, Matthew E. Christman, Edward D. Tourney, Tao Jiang, Sho Ohta, Hui Cheng, Tracy Fitzgerald, Bernd Fritzsch, Sherri M. Jones, Kathleen E. Cullen, Doris K. Wu

Department of Special Education and Communication Disorders: Faculty Publications

Otolith organs of the inner ear are innervated by two parallel afferent projections to the brainstem and cerebellum. These innervations were proposed to segregate across the line of polarity reversal (LPR) within each otolith organ, which divides the organ into two regions of hair cells (HC) with opposite stereociliary orientation. The relationship and functional significance of these anatomical features are not known.Here, we show regional expression of Emx2 in otolith organs, which establishes LPR, mediates the neuronal segregation across LPR and constitutes the bidirectional sensitivity function. Conditional knockout (cKO) of Emx2 in HCs lacks LPR. Tmie cKO, in which mechanotransduction …


Subtype C Hiv-1 Reservoirs Throughout The Body In Art-Suppressed Individuals, Zhou Liu, Peter Julius, Guobin Kang, John T. West, Charles Wood Oct 2022

Subtype C Hiv-1 Reservoirs Throughout The Body In Art-Suppressed Individuals, Zhou Liu, Peter Julius, Guobin Kang, John T. West, Charles Wood

School of Biological Sciences: Faculty Publications

Subtype B HIV-1 reservoirs have been intensively investigated, but reservoirs in other subtypes and how they respond to antiretroviral therapy (ART) is substantially less established. To characterize subtype C HIV-1 reservoirs, we implemented postmortem frozen, as well as formalin fixed paraffin embedded (FFPE) tissue sampling of central nervous system (CNS) and peripheral tissues. HIV-1 LTR, gag, envelope (env) DNA and RNA was quantified using genomic DNA and RNA extracted from frozen tissues. RNAscope was used to localize subtype C HIV-1 DNA and RNA in FFPE tissue. Despite uniform viral load suppression in our cohort, PCR results …


Design And Development Of A Broiler Mortality Removal Robot, Guoming Li, Gary Daniel Chesser, Joseph L. Purswell, Christopher L. Magee, Richard S. Gates, Yijie Xiong Oct 2022

Design And Development Of A Broiler Mortality Removal Robot, Guoming Li, Gary Daniel Chesser, Joseph L. Purswell, Christopher L. Magee, Richard S. Gates, Yijie Xiong

Department of Animal Science: Faculty Publications

Manual collection of broiler mortality is time-consuming, unpleasant, and laborious. The objectives of this research were: (1) to design and fabricate a broiler mortality removal robot from commercially available components to automatically collect dead birds; (2) to compare and evaluate deep learning models and image processing algorithms for detecting and locating dead birds; and (3) to examine detection and mortality pickup performance of the robot under different light intensities. The robot consisted of a two-finger gripper, a robot arm, a camera mounted on the robot’s arm, and a computer controller. The robot arm was mounted on a table, and 64 …


Microbial Community Structure From Southern High Plains Beef Cattle Feedyard Manure And Relationship With Nitrous Oxide Emissions, Heidi Waldrip, David Parker, Sierra Miller, Lisa M. Durso, Byeng R. Min, Daniel N. Miller, Kenneth Casey, Bryan Woodbury, Mindy J. Spiehs Oct 2022

Microbial Community Structure From Southern High Plains Beef Cattle Feedyard Manure And Relationship With Nitrous Oxide Emissions, Heidi Waldrip, David Parker, Sierra Miller, Lisa M. Durso, Byeng R. Min, Daniel N. Miller, Kenneth Casey, Bryan Woodbury, Mindy J. Spiehs

Roman L. Hruska U.S. Meat Animal Research Center: Reports

Modern molecular techniques enable characterization of the microbial biome in livestock manure, from which there is particular concern over emission of greenhouse gases. This study evaluated how sampling depth, time, temperature, and artificial rainfall affected microbial community structure in feedyard manure, and relationships between the manure biome and known parameters related to nitrous oxide (N2O) emissions. In three large incubation chambers, maintained at different temperatures that received two applications of artificial rainfall, we evaluated manure microbiome composition and abundance of N2O-producing enzymes (nirK and nirS) using quantitative polymerase chain reaction (qPCR). These data were …


Accuracies Of Field Co2–H2O Data From Open-Path Eddy-Covariance Flux Systems: Assessment Based On Atmospheric Physics And Biological Environment, Xinhua Zhou, Tian Gao, Ning Zheng, Bai Yang, Yanlei Li, Fengyuan Yu, Tala Awada, Jiaojun Zhu Oct 2022

Accuracies Of Field Co2–H2O Data From Open-Path Eddy-Covariance Flux Systems: Assessment Based On Atmospheric Physics And Biological Environment, Xinhua Zhou, Tian Gao, Ning Zheng, Bai Yang, Yanlei Li, Fengyuan Yu, Tala Awada, Jiaojun Zhu

School of Natural Resources: Faculty Publications

Ecosystem CO2–H2O data measured by infrared gas analyzers in open-path eddy-covariance (OPEC) systems have numerous applications, such as estimations of CO2 and H2O fluxes in the atmospheric boundary layer. To assess the applicability of the data for these estimations, data uncertainties from analyzer measurements are needed. The uncertainties are sourced from the analyzers in zero drift, gain drift, cross-sensitivity, and precision variability. These four uncertainty sources are individually specified for analyzer performance, but so far no methodology exists yet to combine these individual sources into a composite uncertainty for the specification of an …


Whole Genome Sequencing Of Moraxella Bovis Strains From North America Reveals Two Genotypes With Different Genetic Determinants, Emily L. Wynn, Matthew M. Hille, John Dustin Loy, Gennie Schuller, Kristen L. Kuhn, Aaron M. Dickey, James L. Bono, Michael L. Clawson Oct 2022

Whole Genome Sequencing Of Moraxella Bovis Strains From North America Reveals Two Genotypes With Different Genetic Determinants, Emily L. Wynn, Matthew M. Hille, John Dustin Loy, Gennie Schuller, Kristen L. Kuhn, Aaron M. Dickey, James L. Bono, Michael L. Clawson

School of Veterinary and Biomedical Sciences: Faculty Publications

Background: Moraxella bovis and Moraxella bovoculi both associate with infectious bovine keratoconjunctivitis (IBK), an economically significant and painful ocular disease that affects cattle worldwide. There are two genotypes of M. bovoculi (genotypes 1 and 2) that differ in their gene content and potential virulence factors, although neither have been experimentally shown to cause IBK. M. bovis is a causative IBK agent, however, not all strains carry a complete assortment of known virulence factors. The goals of this study were to determine the population structure and depth of M. bovis genomic diversity, and to compare core and accessory genes and …


The Effect Of Species Diversity And Shade On Milkweed Growth And Cardenolide Concentration, Estrella V. Silva, Rylee Mattson, Katherine Klassen, Laurie Dizney Oct 2022

The Effect Of Species Diversity And Shade On Milkweed Growth And Cardenolide Concentration, Estrella V. Silva, Rylee Mattson, Katherine Klassen, Laurie Dizney

RURALS: Review of Undergraduate Research in Agricultural and Life Sciences

Monarch butterflies (Danaus plexippus) rely upon milkweed plants for survival, as it is the only plant upon which female monarchs oviposit and it is the sole food supply for monarch larvae. Additionally, cardenolide, a defense toxin within milkweed tissue, is sequestered by monarchs, making them unpalatable to most predators. Eastern and Western monarch populations in the United States are in sharp decline, in part due to the growing scarcity of milkweed, and reestablishing milkweed habitat is crucial to the monarch’s long-term survival. This study aimed to study the effects of plant species diversity and shade on the growth …


Genomics And Transcriptomics To Protect Rice (Oryza Sativa L.) From Abiotic Stressors: -Pathways To Achieving Zero Hunger, Mushtaq Ahmad Oct 2022

Genomics And Transcriptomics To Protect Rice (Oryza Sativa L.) From Abiotic Stressors: -Pathways To Achieving Zero Hunger, Mushtaq Ahmad

Center for Plant Science Innovation: Faculty Publications

More over half of the world’s population depends on rice as a major food crop. Rice (Oryza sativa L.) is vulnerable to abiotic challenges including drought, cold, and salinity since it grown in semi-aquatic, tropical, or subtropical settings. Abiotic stress resistance has bred into rice plants since the earliest rice cultivation techniques. Prior to the discovery of the genome, abiotic stressrelated genes were identified using forward genetic methods, and abiotic stress-tolerant lines have developed using traditional breeding methods. Dynamic transcriptome expression represents the degree of gene expression in a specific cell, tissue, or organ of an individual organism at …


Seeing Light From A Different Angle: The Effects Of Diffuse Light Environments On The Function, Structure, And Growth Of Tomato Plants, Kendra B.L. Ellertson, Gregory R. Goldsmith, Z. Carter Berry Oct 2022

Seeing Light From A Different Angle: The Effects Of Diffuse Light Environments On The Function, Structure, And Growth Of Tomato Plants, Kendra B.L. Ellertson, Gregory R. Goldsmith, Z. Carter Berry

RURALS: Review of Undergraduate Research in Agricultural and Life Sciences

While considerable attention has been paid to how plants respond to changes in the spectral distribution, less attention has been paid to how plants respond to changes in the angular qualities of light. Evidence from both leaf- and ecosystem-scale measurements indicate that plants vary in their response to diffuse compared to direct light growing environments. Because of the significant implications for agricultural production, we quantified how changes in the angular quality of light affect the structure, function, and growth of Roma tomatoes in a greenhouse experiment with direct and diffuse light treatments. Diffuse light conditions (ca. 50-60% diffuse) were created …


A Long-Term Vision For An Ecologically Sound Platte River, Andrew J. Caven, Melissa M. Mosier, Kristal Stoner, Bill Taddicken, Brice Krohn, Ashley Gramza, Craig R. Allen, Mike Carter, Michelle Koch, Kirk D. Schroeder, Sarah Bailey, Rich Walters, Brian C. Chaffin, Erica Gnuse, Amy Jones, Kate Bird Oct 2022

A Long-Term Vision For An Ecologically Sound Platte River, Andrew J. Caven, Melissa M. Mosier, Kristal Stoner, Bill Taddicken, Brice Krohn, Ashley Gramza, Craig R. Allen, Mike Carter, Michelle Koch, Kirk D. Schroeder, Sarah Bailey, Rich Walters, Brian C. Chaffin, Erica Gnuse, Amy Jones, Kate Bird

Zea E-Books Collection

The Platte River extends about 310 mi (499 km) from North Platte, Nebraska, to its terminus at the Missouri River confluence near Plattsmouth, Nebraska. The Platte River Valley is a continentally significant ecosystem that serves as a major stopover for migratory waterbirds in the Central Flyway including the endangered Whooping Crane (Grus americana) and >1 million Sandhill Cranes (Antigone canadensis) at the peak of spring migration. However, the Platte River Valley also supports a great diversity of avifauna including grassland breeding birds, native stream fish, vascular plants, herpetofauna, mammals, pollinators, and aquatic macroinvertebrates. Despite ongoing conservation …


Fabricating 3-Dimensional Human Brown Adipose Microtissues For Transplantation Studies, Ou Wang, Li Han, Haishuang Lin, Mingmei Tian, Shuyang Zhang, Bin Duan, Soonkyu Chung, Chi Zhang, Xiaojun Lian, Yong Wang, Yuguo Lei Oct 2022

Fabricating 3-Dimensional Human Brown Adipose Microtissues For Transplantation Studies, Ou Wang, Li Han, Haishuang Lin, Mingmei Tian, Shuyang Zhang, Bin Duan, Soonkyu Chung, Chi Zhang, Xiaojun Lian, Yong Wang, Yuguo Lei

Department of Chemical and Biomolecular Engineering: Faculty Publications

Transplanting cell cultured brown adipocytes (BAs) represents a promising approach to prevent and treat obesity (OB) and its associated metabolic disorders, including type 2 diabetes mellitus (T2DM). However, transplanted BAs have a very low survival rate in vivo. The enzymatic dissociation during the harvest of fully differentiated BAs also loses significant cells. There is a critical need for novel methods that can avoid cell death during cell preparation, transplantation, and in vivo. Here, we reported that preparing BAs as injectable microtissues could overcome the problem. We found that 3D culture promoted BA differentiation and UCP-1 expression, and the optimal initial …


Patterns Of Population Structure And Introgression Among Recently Differentiated Drosophila Melanogaster Populations, Jenn M. Coughlan, Andrius J. Dagilis, Antonio Serrato-Capuchina, Hope Elias, David Peede, Kristin Isbell, Dean M. Castillo, Brandon S. Cooper, Daniel R. Matute Oct 2022

Patterns Of Population Structure And Introgression Among Recently Differentiated Drosophila Melanogaster Populations, Jenn M. Coughlan, Andrius J. Dagilis, Antonio Serrato-Capuchina, Hope Elias, David Peede, Kristin Isbell, Dean M. Castillo, Brandon S. Cooper, Daniel R. Matute

Nebraska Cooperative Extension: Faculty and Staff Publications

Despite a century of genetic analysis, the evolutionary processes that have generated the patterns of exceptional genetic and phenotypic variation in the model organism Drosophila melanogaster remains poorly understood. In particular, how genetic variation is partitioned within its putative ancestral range in Southern Africa remains unresolved. Here, we study patterns of population genetic structure, admixture, and the spatial structuring of candidate incompatibility alleles across a global sample, including 223 new accessions, predominantly from remote regions in Southern Africa. We identify nine major ancestries, six that primarily occur in Africa and one that has not been previously described. We find evidence …


Comparative Behavioral Ecotoxicology Of Inland Silverside Larvae Exposed To Pyrethroids Across A Salinity Gradient, Sara J. Hutton, Samreen Siddiqui, Emily I. Pedersen, Christopher Y. Markgraf, Amelie Segarra, Michelle L. Hladik, Richard E. Connon, Susanne M. Brander Oct 2022

Comparative Behavioral Ecotoxicology Of Inland Silverside Larvae Exposed To Pyrethroids Across A Salinity Gradient, Sara J. Hutton, Samreen Siddiqui, Emily I. Pedersen, Christopher Y. Markgraf, Amelie Segarra, Michelle L. Hladik, Richard E. Connon, Susanne M. Brander

United States Geological Survey: Staff Publications

Pyrethroids, a class of commonly used insecticides, are frequently detected in aquatic environments, including estuaries. The influence that salinity has on organism physiology and the partitioning of hydrophobic chemicals, such as pyrethroids, has driven interest in how toxicity changes in saltwater compared to freshwater. Early life exposures in fish to pyrethroids cause toxicity at environmentally relevant concentrations, which can alter behavior. Behavior is a highly sensitive endpoint that influences overall organism fitness and can be used to detect toxicity of environmentally relevant concentrations of aquatic pollutants. Inland Silversides (Menidia beryllina), a commonly used euryhaline model fish species, were exposed …


Updated Perspectives On The Role Of Biomechanics In Copd: Considerations For The Clinician, Jennifer M. Yentes, Wai-Yan Liu, Kuan Zhang, Eric J. Markvicka, Stephen I. Rennard Oct 2022

Updated Perspectives On The Role Of Biomechanics In Copd: Considerations For The Clinician, Jennifer M. Yentes, Wai-Yan Liu, Kuan Zhang, Eric J. Markvicka, Stephen I. Rennard

Department of Electrical and Computer Engineering: Faculty Publications

Patients with chronic obstructive pulmonary disease (COPD) demonstrate extra-pulmonary functional decline such as an increased prevalence of falls. Biomechanics offers insight into functional decline by examining mechanics of abnormal movement patterns. This review discusses biomechanics of functional outcomes, muscle mechanics, and breathing mechanics in patients with COPD as well as future directions and clinical perspectives. Patients with COPD demonstrate changes in their postural sway during quiet standing compared to controls, and these deficits are exacerbated when sensory information (eg, eyes closed) is manipulated. If standing balance is disrupted with a perturbation, patients with COPD are slower to return to baseline …


Proteômica Plasmática Quantitativa De Pacientes Sobreviventes E Não Sobreviventes De Covid-19 Internados No Hospital Revela Potenciais Biomarcadores Prognósticos E Alvos Terapêuticos, D C. Flora, A D. Valle, H Abs Pereira, T F. Garbieri, L. T. Grizzo, T J. Dionisio, A L. Leite, D Mc Rosa, C F. Santos, M Ar Buzalaf Oct 2022

Proteômica Plasmática Quantitativa De Pacientes Sobreviventes E Não Sobreviventes De Covid-19 Internados No Hospital Revela Potenciais Biomarcadores Prognósticos E Alvos Terapêuticos, D C. Flora, A D. Valle, H Abs Pereira, T F. Garbieri, L. T. Grizzo, T J. Dionisio, A L. Leite, D Mc Rosa, C F. Santos, M Ar Buzalaf

Nebraska Center for Virology: Faculty Publications

O desenvolvimento de novas abordagens que permitam a avaliação precoce de quais casos de COVID-19 provavelmente se tornarão críticos e a descoberta de novos alvos terapêuticos são importantes. Neste estudo de coorte, foi avaliado o perfil proteômico e laboratorial do plasma de 163 pacientes internados no Hospital Estadual de Bauru (Bauru, SP, Brasil) entre 4 de maio e 4 de julho de 2020, que foram diagnosticados com COVID-19 por RT-PCR a partir de amostras de swab nasofaríngeo amostras. Amostras de plasma foram coletadas na admissão para análises laboratoriais de rotina e análise proteôomica quantitativa shotgun livre de marcadores. De acordo …


Salt Tolerance Qtls Of An Endemic Rice Landrace, Horkuch At Seedling And Reproductive Stages, Taslima Haque, Sabrina M. Elias, Samsad Razzaque, Sudip Biswas, Sumaiya Farah Khan, G. M. Nurnabi Azad Jewel, Md. Sazzadur Rahman, Thomas E. Juenger, Zeba I. Seraj Oct 2022

Salt Tolerance Qtls Of An Endemic Rice Landrace, Horkuch At Seedling And Reproductive Stages, Taslima Haque, Sabrina M. Elias, Samsad Razzaque, Sudip Biswas, Sumaiya Farah Khan, G. M. Nurnabi Azad Jewel, Md. Sazzadur Rahman, Thomas E. Juenger, Zeba I. Seraj

Department of Agronomy and Horticulture: Faculty Publications

Salinity has a significant negative impact on production of rice. To cope with the increased soil salinity due to climate change, we need to develop salt tolerant rice varieties that can maintain their high yield. Rice landraces indigenous to coastal Bangladesh can be a great resource to study the genetic basis of salt adaptation. In this study, we implemented a QTL analysis framework with a reciprocal mapping population developed from a salt tolerant landrace Horkuch and a high yielding rice variety IR29. Our aim was to detect genetic loci that contributes to the salt adaptive responses of the two …