Digital Commons Network^™

Dynamic Field Programmable Logic-Driven Soft Exosuit, Frances Cleary, Witawas Srisa-An, David C. Henshall, Sasitharan Balasubramaniam

School of Computing: Faculty Publications

The next generation of etextiles foresees an era of smart wearable garments where embedded seamless intelligence provides the ability to sense, process and perform. Core to this vision is embedded textile functionality enabling dynamic configuration. In this paper we detail a methodology, design and implementation of a dynamic field programmable logic-driven fabric soft exosuit. Dynamic field programmability allows the soft exosuit to alter its functionality and adapt to specific exercise programs depending on the wearers need. The dynamic field programmability is enabled through motion based control arm movements of the soft exosuit triggering momentary sensors embedded in the fabric exosuit …

Creative Connections: A Guidebook For Arts-Based Inquiry, Julia Marshall, Kimberley D'Adamo, Ann Ledo Lane

Kimberley D'Adamo: Art, Curriculum, Publications, and Other Scholarly Works

Art-based inquiry is an approach to art education that frames art making as a form of inquiry—a way of exploring and learning about ideas, ourselves and the world. The goals of art-based inquiry are understanding important topics, imagining possibilities, and inventing new realities. Students create art in order to meet those goals and they do it with a teacher’s guidance. For Creative Connections, art-based inquiry calls for a slight shift in perspective and change in practice. Adopting art-based inquiry does not mean throwing out everything you do. It means seeing art class diff erently and adapting it to a more …

Fgf Ligands And Receptors In Osteochondral Tissues Of The Temporomandibular Joint In Young And Aging Mice, Eliane H. Dutra, Po-Jung Chen, Zana Kalajzic, Sunil Wadhwa, Marja Hurley, Sumit Yadav

College of Dentistry: Faculty Publications

Objective. Fibroblast growth factors (FGFs) are a family of 22 proteins and 4 FGF receptors (FGFRs) that are crucial elements for normal development. The contribution of different FGFs and FGFRs for the homeostasis or disease of the cartilage from the mandibular condyle is unknown. Therefore, our goal was to characterize age-related alterations in the protein expression of FGF ligands and FGFRs in the mandibular condyle of mice. Method. Mandibular condyles of 1-, 6-, 12-, 18-, and 24-month-old C57BL/6J male mice (5 per group) were collected and histologically sectioned. Immunofluorescence for FGFs that have been reported to be relevant …

A Method For Successful Collection Of Multicores And Gravity Cores From Antarctic Subglacial Lakes, Brad E. Rosenheim, Alexander B. Michaud, James Broda, Alan Gagnon, Ryan A. Venturelli, Timothy D. Campbell, Amy Leventer, Molly Patterson, Matthew J. Siegfried, Brent C. Christner, Dennis Duling, David M. Harwood, John E. Dore, Martyn Tranter, Mark L. Skidmore, John C. Priscu, Salsa Science Team

Department of Earth and Atmospheric Sciences: Faculty Publications

During the 2018–2019 Antarctic field season, the Subglacial Antarctic Lakes Scientific Access project team cleanly accessed Mercer Subglacial Lake, West Antarctica, to sample water and sediments beneath 1087 m of overlying ice. A multicorer was successful in sampling the sediment–water interface, with 4 deployments retrieving 10 cores between 0.3 and 0.4 m in length. Gravity coring was also successful, retrieving cores of 0.97 and 1.78 m in glacial diamict. However, sediment cores retrieved by the gravity cores were shorter than the core barrel penetration (as measured by mud streaks on the outside of the coring system), indicating that the system …

Exploring The Impact Of Pediatric Short Bowel Syndrome On Parent Well‐Being Using A Disease‐Specific Pilot Survey, Marie L. Neumann, Jessica Y. Allen, Amy Ladner, Swapna Kakani, Meaghann S. Weaver, David F. Mercer

Department of Communication Studies: Faculty Publications

Background: Children with short bowel syndrome (SBS) have complex care needs, most of which are met in the home by family caregivers who may experience a range of stressors unique to this experience. Prior research suggests that parents of children with SBS have poorer health‐related quality of life than peers parenting children without health needs, but the mechanisms shaping parent outcomes are understudied.

Methods: A pilot survey was developed using a community‐driven research design to measure the impact of disease‐specific items on parent‐perceived well‐being. The cross‐sectional survey, which included both closed‐ended and open‐ended items, was distributed to a …

Characterization Of Western Corn Rootworm (Coleoptera: Chrysomelidae) Susceptibility To Foliar Insecticides In Northeast Nebraska, Timothy B. Dang, Ana M. Vélez, Arnubio Valencia-Jiménez, Jordan Reinders, Emily E. Stricklin, Matthew W. Carroll, Clinton D. Pilcher, Jeffrey T. Krumm, Graham P. Head, Lance Meinke

Department of Entomology: Faculty Publications

Foliar-applied insecticides are commonly used for adult Western Corn Rootworm (WCR), Diabrotica virgifera virgifera LeConte (Coleoptera: Chrysomelidae), control in Nebraska but little efficacy data is available. Anecdotal reports of reduced efficacy in areas of northeast Nebraska led to the conduct of this study. Objectives were to (i) evaluate the efficacy of commercial applications of commonly used formulated insecticides (bifenthrin, lambda-cyhalothrin, chlorpyrifos, or tank mixes) for WCR control in 7 northeast Nebraska counties during 2019 and 2020 and (ii) conduct adult WCR concentration-response vial bioassays with bifenthrin, chlorpyrifos, and dimethoate active ingredients on a subset of field populations. Whole plant counts …

Insect And Pest Management For Sustaining Crop Production Under Changing Climatic Patterns Of Drylands, Ruchika Kashyap, Sajjan Grover, Heena Puri, Sandeep Kaur, Jagmohan Singh, Karansher S. Sandhu, Gagandeep Kaur, Hinal Kharva, Shivreet Kaur, Balwinder Kaur

Department of Entomology: Faculty Publications

Climate change is alarming, particularly for agriculturists as it severely impacts the development, distribution, and survival of insects and pests, affecting crop production globally. Over time, climate change is drastically tumbling the crop productivity in all the cropping systems, whereas the dryland agriculture with existing low productivity is immensely hit. While all the existing species in drylands, including humans, are coping with extreme climate variations for millennia, future climate change predictions put dryland agriculture in a threat zone. Drylands support 38% of the world’s population; therefore, climate change coupled with population growth and global food security draws the attention of …

Indigenous Cultural Identity Protects Against Intergenerational Transmission Of Aces Among Indigenous Caregivers And Their Children, Katie Edwards, Emily A. Waterman, Natira Mullet, Ramona Herrington, Sloane Cornelius, Skyler Hopfauf, Preciouse Trujillo, Lorey A. Wheeler, Arielle R. Deutsch

Department of Educational Psychology: Faculty Publications

A large body of empirical research has demonstrated that caregiver adverse childhood experiences (ACEs) predict ACEs in one’s child, a phenomenon known as the intergenerational transmission of ACEs. Little of this empirical research, however, has focused specifically on Indigenous peoples despite a growing body of theoretical literature and the wisdom of Elders and Traditional Knowledge Keepers that speaks to the presence of this phenomenon within Indigenous communities as well as the protective role of Indigenous cultural identity in preventing the intergenerational transmission of ACEs. The purpose of the current study was to conduct an empirical evaluation of this hypothesis, specifically …

Early-Phase Drive To The Precursor Pool: Chloroviruses Dive Into The Deep End Of Nucleotide Metabolism, David Dunigan, Irina Agarkova, Ahmed Esmael, Sophie Alvarez, James L. Van Etten

Nebraska Center for Virology: Faculty Publications

Viruses face many challenges on their road to successful replication, and they meet those challenges by reprogramming the intracellular environment. Two major issues challenging Paramecium bursaria chlorella virus 1 (PBCV-1, genus Chlorovirus, family Phycodnaviridae) at the level of DNA replication are (i) the host cell has a DNA G+C content of 66%, while the virus is 40%; and (ii) the initial quantity of DNA in the haploid host cell is approximately 50 fg, yet the virus will make approximately 350 fg of DNA within hours of infection to produce approximately 1000 virions per cell. Thus, the quality and quantity of …

The Persistence And Stabilization Of Auxiliary Genes In The Human Skin Virome, Ema H. Graham, Wesley A. Tom, Alison C. Neujahr, Michael Adamowicz, Jennifer Clarke, Joshua R. Herr, Samodha C. Fernando

Nebraska Center for Virology: Faculty Publications

Background The human skin contains a diverse microbiome that provides protective functions against environmental pathogens. Studies have demonstrated that bacteriophages modulate bacterial community composition and facilitate the transfer of host-specific genes, potentially influencing host cellular functions. However, little is known about the human skin virome and its role in human health. Especially, how viral-host relationships influence skin microbiome structure and function is poorly understood.

Results Population dynamics and genetic diversity of bacteriophage communities in viral metagenomic data collected from three anatomical skin locations from 60 subjects at five different time points revealed that cutaneous bacteriophage populations are mainly composed of …

Elections And Post Traumatic Stress: Evidence From The 2020 U.S. Presidential Election, Timothy Fraser, Costas Panagopoulos, Kevin Smith

Department of Political Science: Faculty Publications

The 2020 U.S. presidential election saw rising political tensions among ordinary voters and political elites, with fears of election violence culminating in the January 6th riot. We hypothesized that the 2020 election might have been traumatic for some voters, producing measurable symptoms of post-traumatic stress disorder (PTSD). We also hypothesized that negative sentiment towards the opposing party correlates with PTSD. We measured PTSD with a modified PCL-5, a validated PTSD screener, for 573 individuals from a nationally representative YouGov sample. We modeled the association between affective polarization and PTSD, controlling for political, demographic, and psychological traits. We estimate that 12.5% …

Helminth And Protozoan Parasites Of Subterranean Rodents (Chordata, Mammalia, Rodentia) Of The World, Altangerel Tsogtsaikhan Dursahinhan, Daniel A. Kenkel, Scott Lyell Gardner

Scott L. Gardner Publications

Published studies and ten new unpublished records included herein reveal that approximately 174 species of endoparasites (helminths and protozoans) are known from 65 of 163 species of rodents that occupy the subterranean ecotope globally. Of those, 94 endoparasite species were originally described from these rodents. A total of 282 host-parasite associations are summarized from four major zoogeographic regions including Ethiopian, Palearctic/Oriental, Nearctic, and Neotropical. Thirty-four parasite records from the literature have been identified to only the level of the genus. In this summary, ten new records have been added, and the most current taxonomic status of each parasite species is …

Intercontinental Comparisons Of Subterranean Host-Parasite Communities Using Bipartite Network Analyses, Altangerel Tsogtsaikhan Dursahinhan, Sebastian Botero-Cañola, Scott L. Gardner

Scott L. Gardner Publications

Rodents living in a subterranean ecotope face a unique combination of evolutionary and ecological pressures and while host species evolution may be driven by the selective pressure from the parasites they harbour, the parasites may be responding to the selective pressures of the host. Here we obtained all available subterranean rodent host-parasite records from the literature and integrated these data by utilizing a bipartite network analysis to determine multiple critical parameters to quantify and measure the structure and interactions of the organisms present in host-parasite communities. A total of 163 species of subterranean rodent hosts, 174 parasite species, and 282 …

“Revisiting The Past”: A Redescription Of Physaloptera Retusa (Nemata, Physalopteridae) From Material Deposited In Museums And New Material From Amazon Lizards = “Revisitando O Passado”: Uma Redescrição De Physaloptera Retusa (Nemata, Physalopteridae) A Partir De Material Depositado Em Museus E Novo Material De Lagartos Amazônicos, Lílian Cristina Macedo, Yuri Willkens, Leandro Maurício Oliveira Da Silva, Scott Lyell Gardner, Francisco Tiago De Vasconcelos Melo, Jeannie Nascimento Dos Santos

Scott L. Gardner Publications

Abstract

Physaloptera Rudolphi, 1819 is a genus of nematodes that includes approximately 100 species parasitic in vertebrates around the world. From these, approximately 30 occur in the Neotropical region, with nine reported from neotropical reptiles. Physaloptera spp. are recognized by their distinct morphology of the apical end and characters of the reproductive system. However, despite the fact that the morphological characters for species diagnosis have been firmly established, we frequently find identification problems regarding poorly detailed descriptions and poorly preserved specimens. These may lead to taxonomic incongruencies. Physaloptera retusa (Rudolphi, 1819) is the most common species of the genus and …

Tapping Into Natural History Collections To Assess Latitudinal Gradients Of Parasite Diversity, Sebastian Botero-Cañola, Scott Lyell Gardner

Scott L. Gardner Publications

Parasites are key components of the biosphere not only due to their huge diversity, but also because they exert important influences on ecological processes. Nevertheless, we lack an understanding of the biogeographical patterns of parasite diversity. Here, we tap into the potential of biodiversity collections for understanding parasite biogeography. We assess species richness of supracommunities of helminth parasites infecting mammal assemblages in the Nearctic, and describe its relation to latitude, climate, host diversity, and land area. We compiled data from parasitology collections and assessed parasite diversity in Nearctic ecoregions for the entire parasite supracommunity of mammals in each ecoregion, as …

Filling The Gap In Distribution Ranges And Conservation Status In Ctenomys (Rodentia: Ctenomyidae), Diego A. Caraballo, Sabrina Laura Lopez, Sebastian Botero-Cañola, Scott Lyell Gardner

Scott L. Gardner Publications

South American subterranean rodents of the genus Ctenomys (Rodentia, Ctenomyidae, tuco-tuco) are one of the most diverse genera among mammals. Recently described species, new taxonomic revisions, and new distribution range delimitation made the revision of distribution areas and conservation status of these mammals mandatory. Implementing the first part of the DAMA protocol (document, assess, monitor, act), here we compile updated sets of species distribution range maps and use these and the number of collection localities to assess the conservation status of ctenomyids. We integrate potential for conservation in protected areas, and levels of habitat transformation to revise previous conservation status …

A New Species Of Mathevotaenia (Cestoda: Anoplocephalidae) From The Andean Tuco-Tuco, Ctenomys Opimus (Rodentia: Ctenomyidae), On The Altiplano Of Bolivia, Scott Lyell Gardner, Bennett A. Grappone, Alex Lai

Scott L. Gardner Publications

A new species of Mathevotaenia Akumyan, 1946 (Cestoda: Anoplocephalidae) is described from the Andean tuco-tuco, Ctenomys opimus Wagner 1848 (Rodentia: Ctenomyidae), collected in 1984 on the Altiplano of Bolivia. This is the second species of anoplocephalid cestode recorded from rodents of the genus Ctenomys, the first being Monoecocestus torresi Olsen 1976 from the Maule tuco-tuco, Ctenomys maulinus Philippi 1872, documented in southwestern Argentina. The new species of Mathevotaenia described here has more testes per segment than any other described species of the same genus from South America. The description of a new species from a decades-old specimen highlights the …

Mites [Of Dogs And Cats], Susan E. Little, Roberto Cortinas

School of Veterinary and Biomedical Sciences: Faculty Publications

Veterinary parasitology: 20,000-word article details recognition and treatments for mite infestations of dogs and cats. Mite species reviewed include:

Cheyletiella spp. (“Walking Dandruff”)

Demodex spp. (Hair Follicle Mites, Red Mange, Demodicosis, Demodectic Mange)

Sarcoptes scabiei (Itch Mite, Scabies, Sarcoptic Mange)

Notoedres cati (Feline Head and Neck Mange)

Otodectes cynotis (Ear Mites)

Pneumonyssoides caninum (Nasal Mite)

Lynxacarus radovskyi (Fur Mite, Hair Clasping Mite)

Trombiculid Mites (Chiggers, Red Bugs, Berry Bugs, Harvest Mites, Scrub Itch or Grass Itch Mites)

Discussions include Etiology and epidemiology, Pathogenesis, Clinical features, Diagnosis, Treatment and prognosis, Immunity, Prevention, and Public health aspects.

Complete with 209 references.

More People, More Cats, More Parasites: Human Population Density And Temperature Variation Predict Prevalence Of Toxoplasma Gondii Oocyst Shedding In Free-Ranging Domestic And Wild Felids, Sophie Zhu, Elizabeth Vanwormer, Karen Shapiro

School of Veterinary and Biomedical Sciences: Faculty Publications

Toxoplasma gondii is a ubiquitous zoonotic parasite that can infect warm-blooded vertebrates, including humans. Felids, the definitive hosts, drive T. gondii infections by shedding the environmentally resistant stage of the parasite (oocysts) in their feces. Few studies characterize the role of climate and anthropogenic factors in oocyst shedding among free-ranging felids, which are responsible for the majority of environmental contamination. We determined how climate and anthropogenic factors influence oocyst shedding in free-ranging domestic cats and wild felids using generalized linear mixed models. T. gondii oocyst shedding data from 47 studies were systematically reviewed and compiled for domestic cats and six …

Chemical Characterization And Biological Evaluation Of Epilobium Parviflorum Extracts In An In Vitro Model Of Human Malignant Melanoma, Sotiris Kyriakou, Venetia Tragkola, Ioannis Paraskevaidis, Mihalis Plioukas, Dimitrios T. Trafalis, Rodrigo Franco, Aglaia Pappa, Mihalis I. Panayiotidi

School of Veterinary and Biomedical Sciences: Faculty Publications

Malignant melanoma is an aggressive type of skin cancer characterised by high metastatic capacity and mortality rate. On the other hand, Epilobium parviflorum is known for its medicinal properties, including its anticancer potency. In this context, we aimed to (i) isolate various extracts of E. parviflorum, (ii) characterize their phytochemical content, and (iii) determine their cytotoxic potential in an in vitro model of human malignant melanoma. To these ends, we utilized various spectrophotometric and chromatographic (UPLC-MS/MS) approaches to document the higher content of the methanolic extract in polyphenols, soluble sugars, proteins, condensed tannins, and chlorophylls -a and -b as …

Does Swab Type Matter? Comparing Methods For Mannheimia Haemolytica Recovery And Upper Respiratory Microbiome Characterization In Feedlot Cattle, William B. Crosby, Lee Pinnell, John T. Richeson, Cory Wolfe, Jake Castle, John Dustin Loy, Sheryl P. Gow, Keun Seok Seo, Sarah F. Capik, Amelia R. Woolums, Paul S. Morley

School of Veterinary and Biomedical Sciences: Faculty Publications

Background: Bovine respiratory disease (BRD) is caused by interactions among host, environment, and pathogens. One standard method for antemortem pathogen identification in cattle with BRD is deep-guarded nasopharyngeal swabbing, which is challenging, costly, and waste generating. The objective was to compare the ability to recover Mannheimia haemolytica and compare microbial community structure using 29.5 inch (74.9 cm) deep-guarded nasopharyngeal swabs, 16 inch (40.6 cm) unguarded proctology swabs, or 6 inch (15.2 cm) unguarded nasal swabs when characterized using culture, real time-qPCR, and 16S rRNA gene sequencing. Samples for aerobic culture, qPCR, and 16S rRNA gene sequencing were collected from the …

Detection Of Mannheimia Haemolytica-Specific Igg, Igm And Iga In Sera And Their Relationship To Respiratory Disease In Cattle, Korakrit Poonsuk, Carita Kordik, Matthew M. Hille, Ting-Yu Cheng, William B. Crosby, Amelia R. Woolums, Michael L. Clawson, Carol G. Chitko-Mckown, Bruce Brodersen, John Dustin Loy

School of Veterinary and Biomedical Sciences: Faculty Publications

Mannheimia haemolytica is one of the major causes of bovine respiratory disease in cattle. The organism is the primary bacterium isolated from calves and young cattle affected with enzootic pneumonia. Novel indirect ELISAs were developed and evaluated to enable quantification of antibody responses to whole cell antigens using M. haemolytica A1 strain P1148. In this study, the ELISAs were initially developed using sera from both M. haemolytica-culture-free and clinically infected cattle, then the final prototypes were tested in the validation phase using a larger set of known-status M. haemolytica sera (n = 145) collected from feedlot cattle. The test …

First Gene-Edited Calf With Reduced Susceptibility To A Major Viral Pathogen, Aspen M. Workman, Michael P. Heaton, Brian L. Vander Ley, Dennis A. Webster, Luke Sherry, Jonathan R. Bostrom, Sabrina Larson, Theodore S. Kalbfleisch, Gregory P. Harhay, Erin E. Jobman, Daniel F. Carlson, Tad S. Sonstegard

School of Veterinary and Biomedical Sciences: Faculty Publications

Bovine viral diarrhea virus (BVDV) is one of the most important viruses affecting the health and well-being of bovine species throughout the world. Here, we used CRISPR-mediated homology-directed repair and somatic cell nuclear transfer to produce a live calf with a six amino acid substitution in the BVDV binding domain of bovine CD46. The result was a gene-edited calf with dramatically reduced susceptibility to infection as measured by reduced clinical signs and the lack of viral infection in white blood cells. The edited calf has no off-target edits and appears normal and healthy at 20 months of age without obvious …

Aerobic Exercise And Scaffolds With Hierarchical Porosity Synergistically Promote Functional Recovery Post Volumetric Muscle Loss, Christina Zhu, Karina Skylar, Yori Endo, Mohamadmahdi Samandari,, Mehran Karvar, Azadeh Mostafavi, Jacob Quint, Chiara Rioldi, Wojciech Swieszkowski, Joshua Mauney, Shailesh Agarwal, Ali Tamayol, Indranil Sinha

School of Veterinary and Biomedical Sciences: Faculty Publications

Volumetric muscle loss (VML) is a composite loss of skeletal muscle tissue (greater than 20%) that heals with minimal muscle regeneration, substantial fibrosis, and subsequent functional deficits. Standard treatment, involving free functional muscle transfer and physical therapy, cannot restore full muscle function following VML. Tissue engineered scaffolds, 3D structural templates that mimic native extracellular matrix, are promising to enhance functional muscle formation and recovery. Bioprinted 3D scaffolds are engineered using bioinks, created from scaffolding material, cells, and growth factors, to replicate skeletal muscle architecture with precise control over their spatial deposition. METHODS: The present study evaluates a 3D-printed foam-like scaffold …

Host-Genetic-Based Outcome Of Co-Infection By Pcv2b And Prrsv In Pigs, Christian W. Eaton, Hiep Vu, Arabella L. Hodges, Seth P. Harris, Stephen D. Kachman, Daniel C. Ciobanu

School of Veterinary and Biomedical Sciences: Faculty Publications

Replication of porcine circovirus type 2 (PCV2), an important worldwide swine pathogen, has been demonstrated to be influenced by host genotype. Specifically, a missense DNA polymorphism (SYNGR2 p.Arg63Cys) within the SYNGR2 gene was demonstrated to contribute to variation in PCV2b viral load and subsequent immune response following infection. PCV2 is known to induce immunosuppression leading to an increase in susceptibility to subsequent infections with other viral pathogens such as porcine reproductive and respiratory syndrome virus (PRRSV). In order to assess the role of SYNGR2 p.Arg63Cys in co-infections, pigs homozygous for the favorable SYNGR2 p.63Cys (N = 30) …

Infection Of Feral Phenotype Swine With Japanese Encephalitis Virus, So Lee Park, Yan-Jang S. Huang, Amy C. Lyons, Victoria B. Ayers, Susan M. Hettenbach, D. Scott Mcvey, Leela E. Noronha, Kenneth R. Burton, Stephen Higgs, Dana L. Vanlandingham

School of Veterinary and Biomedical Sciences: Faculty Publications

Background: Japanese encephalitis virus (JEV) is a mosquito-borne zoonotic flavivirus and the leading cause of pediatric encephalitis in the Asian Pacific region. The transmission cycle primarily involves Culex spp. mosquitoes and Ardeid birds, with domestic pigs (Sus scrofa domestica) being the source of infectious viruses for the spillover of JEV from the natural endemic transmission cycle into the human population. Although many studies have concluded that domestic pigs play an important role in the transmission cycle of JEV, and infection of humans, the role of feral pigs in the transmission of JEV remains unclear. Since domestic and …

Femtosecond Electronic And Hydrogen Structural Dynamics In Ammonia Imaged With Ultrafast Electron Diffraction, Elio G. Champenois, Nanna H. List, Matthew Ware, Mathew Britton, Philip H. Bucksbaum, Xinxin Cheng, Martin Centurion, James P. Cryan, Ruaridh Forbes, Ian Gabalski, Kareem Hegazy, Matthias C. Hoffmann, Andrew J. Howard, Fuhao Ji, Ming-Fu Lin, J. Pedro F. Nunes, Xiaozhe Shen, Jie Yang, Xijie Wang, Todd J. Martinez, Thomas J. A. Wolf

Department of Physics and Astronomy: Faculty Publications

Directly imaging structural dynamics involving hydrogen atoms by ultrafast diffraction methods is complicated by their low scattering cross sections. Here we demonstrate that megaelectronvolt ultrafast electron diffraction is sufficiently sensitive to follow hydrogen dynamics in isolated molecules. In a study of the photodissociation of gas phase ammonia, we simultaneously observe signatures of the nuclear and corresponding electronic structure changes resulting from the dissociation dynamics in the time-dependent diffraction. Both assignments are confirmed by ab initio simulations of the photochemical dynamics and the resulting diffraction observable. While the temporal resolution of the experiment is insufficient to resolve the dissociation in time, …

Increased Liver Stiffness Promotes Hepatitis B Progression By Impairing Innate Immunity In Ccl4-Induced Fibrotic Hbv+ Transgenic Mice, Grace Bybee, Youra Moeun, Weimin Wang, Kusum K. Kharbanda, Larisa Y. Poluektova, Srivatsan Kidambi, Natalia A. Osna, Murali Ganesan

Department of Chemical and Biomolecular Engineering: Faculty Publications

Background: Hepatitis B virus (HBV) infection develops as an acute or chronic liver disease, which progresses from steatosis, hepatitis, and fibrosis to end-stage liver diseases such as cirrhosis and hepatocellular carcinoma (HCC). An increased stromal stiffness accompanies fibrosis in chronic liver diseases and is considered a strong predictor for disease progression. The goal of this study was to establish the mechanisms by which enhanced liver stiffness regulates HBV infectivity in the fibrotic liver tissue. Methods: For in vitro studies, HBV-transfected HepG2.2.15 cells were cultured on polydimethylsiloxane gels coated by polyelectrolyte multilayer films of 2 kPa (soft) or 24 kPa (stiff) …

Mesenchymal Stromal Cells And Alpha-1 Antitrypsin Have A Strong Synergy In Modulating Inflammation And Its Resolution, Li Han, Xinran Wu, Ou Wang, Xiao Luan, William Velander, Michael Aynardi, E. Scott Halstead, Anthony S. Bonavia, Rong Jin, Guohong Li, Yulong Li, Yong Wang, Cheng Dong, Yuguo Lei

Department of Chemical and Biomolecular Engineering: Faculty Publications

Rationale: Trauma, surgery, and infection can cause severe inflammation. Both dysregulated inflammation intensity and duration can lead to significant tissue injuries, organ dysfunction, mortality, and morbidity. Anti-inflammatory drugs such as steroids and immunosuppressants can dampen inflammation intensity, but they derail inflammation resolution, compromise normal immunity, and have significant adverse effects. The natural inflammation regulator mesenchymal stromal cells (MSCs) have high therapeutic potential because of their unique capabilities to mitigate inflammation intensity, enhance normal immunity, and accelerate inflammation resolution and tissue healing. Furthermore, clinical studies have shown that MSCs are safe and effective. However, they are not potent enough, alone, to …

Correction To: Genetic Characterization And Expression Analysis Of Wheat (Triticum Aestivum) Line 07or1074 Exhibiting Very Low Polyphenol Oxidase (Ppo) Activity, S. M. Hystad, J. M. Martin, R. A. Graybosch, M. J. Giroux

Department of Agronomy and Horticulture: Faculty Publications

The above mentioned article was published in 2015 with an error in the naming of one of the allele sequences for the PPO gene on the D genome. The PPO-D1f allele was incorrectly named as it is identical to the PPO-D1c allele previously published in GenBank by the authors on March 11th, 2014. An alignment between the previously published PPOD1c allele and a resequencing of the parental line 07OR1074 shows that the PPO-D1f allele is identical to the PPO-D1c allele. The allele in the paper referred to as PPO-D1f should be treated as PPO-D1c and can be accessed at NCBI …