Digital Commons Network^™

Engineering Neurotoxin-Functionalized Exosomes For Targeted Delivery To The Peripheral Nervous System, Mena Asha Krishnan, Olawale A. Alimi, Tianshu Pan, Mitchell Kuss, Zeljka Korade, Guoku Hu, Bo Liu, Bin Duan

Department of Mechanical and Materials Engineering: Faculty Publications

The administration of therapeutics to peripheral nerve tissue is challenging due to the complexities of peripheral neuroanatomy and the limitations imposed by the blood–nerve barrier (BNB). Therefore, there is a pressing need to enhance delivery effectiveness and implement targeted delivery methods. Recently, erythrocyte-derived exosomes (Exos) have gained widespread attention as biocompatible vehicles for therapeutics in clinical applications. However, engineering targeted Exos for the peripheral nervous system (PNS) is still challenging. This study aims to develop a targeted Exo delivery system specifically designed for presynaptic terminals of peripheral nerve tissue. The clostridium neurotoxin, tetanus toxin-C fragment (TTC), was tethered to the …

Diagnosis Of Disease Affecting Gait With A Body Acceleration‑Based Model Using Reflected Marker Data For Training And A Wearable Accelerometer For Implementation, Mohammad Ali Takallou, Farahnaz Fallahtafti, Mahdi Hassan, Ali Al‑Ramini, Basheer Qolomany, Iraklis I. Pipinos, Sara A. Myers, F. M. Alsaleem

Durham School of Architectural Engineering and Construction: Faculty Publications

This paper demonstrates the value of a framework for processing data on body acceleration as a uniquely valuable tool for diagnosing diseases that affect gait early. As a case study, we used this model to identify individuals with peripheral artery disease (PAD) and distinguish them from those without PAD. The framework uses acceleration data extracted from anatomical reflective markers placed in different body locations to train the diagnostic models and a wearable accelerometer carried at the waist for validation. Reflective marker data have been used for decades in studies evaluating and monitoring human gait. They are widely available for many …

Twenty-Eight Years Of Gm Food And Feed Without Harm: Why Not Accept Them?, Richard E. Goodman

Department of Food Science and Technology: Faculty Publications

Since the first genetically engineered or modified crops or organisms (GMO) were approved for commercial production in 1995, no new GMO has been proven to be a hazard or cause harm to human consumers. These modifications have improved crop efficiency, reduced losses to insect pests, reduced losses to viral and microbial plant pathogens and improved drought tolerance. A few have focused on nutritional improvements producing beta carotene in Golden Rice. Regulators in the United States and countries signing the CODEX Alimentarius and Cartagena Biosafety agreements have evaluated human and animal food safety considering potential risks of allergenicity, toxicity, nutritional and …

Twenty-Eight Years Of Gm Food And Feed Without Harm: Why Not Accept Them?, Richard E. Goodman

Department of Food Science and Technology: Faculty Publications

Since the first genetically engineered or modified crops or organisms (GMO) were approved for commercial production in 1995, no new GMO has been proven to be a hazard or cause harm to human consumers. These modifications have improved crop efficiency, reduced losses to insect pests, reduced losses to viral and microbial plant pathogens and improved drought tolerance. A few have focused on nutritional improvements producing beta carotene in Golden Rice. Regulators in the United States and countries signing the CODEX Alimentarius and Cartagena Biosafety agreements have evaluated human and animal food safety considering potential risks of allergenicity, toxicity, nutritional and …

Yap Mechanotransduction Under Cyclic Mechanical Stretch Loading For Mesenchymal Stem Cell Osteogenesis Is Regulated By Rock, Eunju Kim, Brandon D. Riehl, Tasneem Bouzid, Ruiguo Yang, Bin Duan, Henry J. Donahue, Jung Yul Lim

Department of Mechanical and Materials Engineering: Faculty Publications

While yes-associated protein (YAP) is now recognized as a potent mechanosensitive transcriptional regulator to affect cell growth and differentiation including the osteogenic transcription of mesenchymal stem cells (MSCs), most studies have reported the YAP mechanosensing of static mechanophysical cues such as substrate stiffness. We tested MSC response to dynamic loading, i.e., cyclic mechanical stretching, and assessed YAP mechanosensing and resultant MSC osteogenesis. We showed that cyclic stretching at 10% strain and 1 Hz frequency triggered YAP nuclear import in MSCs. YAP phosphorylation at S127 and S397, which is required for YAP cytoplasmic retention, was suppressed by cyclic stretch. We also …

Antibiotic-Induced Gut Dysbiosis Elicits Gut-Brain Axis Relevant Multi-Omic Signatures And Behavioral And Neuroendocrine Changes In A Nonhuman Primate Model, Shivdeep S. Hayer, Mackenzie Conrin, Jeffrey French, Andrew K. Benson, Sophie Alvarez, Kathryn Dempsey, Anne Fischer, Zahraa Wajih Alsafwani, William Gasper, Mallory J. Suhr Van Haute, Haley R. Hassenstab, Shayda Azadmanesh, Missy Briardy, Skyler Gerbers, Aliyah Jabenis, Jennifer L. Thompson, Jonathan B. Clayton

Food for Health: Publications

Emerging evidence indicates that antibiotic-induced dysbiosis can play an etiological role in the pathogenesis of neuropsychiatric disorders. However, most of this evidence comes from rodent models. The objective of this study was to evaluate if antibiotic-induced gut dysbiosis can elicit changes in gut metabolites and behavior indicative of gut-brain axis disruption in common marmosets (Callithrix jacchus) – a nonhuman primate model often used to study sociability and stress. We were able to successfully induce dysbiosis in marmosets using a custom antibiotic cocktail (vancomycin, enrofloxacin and neomycin) administered orally for 28 days. This gut dysbiosis altered gut metabolite profiles, …

Naturally Derived Phenethyl Isothiocyanate Modulates Induction Of Oxidative Stress Via Its N-Acetylated Cysteine Conjugated Form In Malignant Melanoma, Sotiris Kyriakou, Nikoletta Demosthenous, Tom Amery, Kyle J. Stewart, Paul G. Winyard, Rodrigo Franco, Aglaia Pappa, Mihalis I. Panayiotidis

School of Veterinary and Biomedical Sciences: Faculty Publications

Phenethyl isothiocyanate (PEITC) is a secondary metabolic product yielded upon the hydrolysis of gluconasturtiin and it is highly accumulated in the flowers of watercress. The aim of the current study was to assess the role of a naturally derived PEITC-enriched extract in the induction of oxidative stress and to evaluate its anti-melanoma potency through the regulation of its metabolism with the concurrent production of the N-acetyl cysteine conjugated by-product. For this purpose, an in vitro melanoma model was utilized consisting of human primary (A375) cells as well as metastatic (COLO-679) malignant melanoma cells together with non-tumorigenic immortalized keratinocytes (HaCaT). …

Enhancing Estimation Of Cover Crop Biomass Using Field-Based High-Throughput Phenotyping And Machine Learning Models, Geng Bai, Katja Koehler-Cole, David Scoby, Vesh R. Thapa, Andrea D. Basche, Yufeng Ge

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Incorporating cover crops into cropping systems offers numerous potential benefits, including the reduction of soil erosion, suppression of weeds, decreased nitrogen requirements for subsequent crops, and increased carbon sequestration. The aboveground biomass (AGB) of cover crops strongly influences their performance in delivering these benefits. Despite the significance of AGB, a comprehensive field-based high-throughput phenotyping study to quantify AGB of multiple cover crops in the U.S. Midwest has not been found. This study presents a two-year field experiment carried out in Eastern Nebraska, USA, to estimate AGB of five different cover crop species [canola (Brassica napus L.), rye (Secale …

Course Portfolio For Biochemistry 1: Structure And Metabolism (Bioc431), Didier Mena

UNL Faculty Course Portfolios

This benchmark portfolio encapsulates a comprehensive exploration aimed at enhancing the educational landscape of the BIOC431 course, a part of the general biochemistry course series (431 and 432). These courses are designed to offer a general introduction to the structure and function of cells in the body, along with their chemical reactions. Specifically, BIOC431 focuses on the structure, function, and metabolism of proteins, lipids, carbohydrates, and other major metabolic pathways. The three primary objectives addressed in this portfolio were the reevaluation of learning objectives, reassessment of assessment methods, and documentation of effective classroom strategies. Through background design, the learning objectives …

Vibroscape Analysis Reveals Acoustic Niche Overlap And Plastic Alteration Of Vibratory Courtship Signals In Ground-Dwelling Wolf Spiders, Noori Choi, Pat Miller, Eileen A. Hebets

School of Biological Sciences: Faculty Publications

To expand the scope of soundscape ecology to encompass substrate-borne vibrations (i.e. vibroscapes), we analyzed the vibroscape of a deciduous forest floor using contact microphone arrays followed by automated processing of large audio datasets. We then focused on vibratory signaling of ground-dwelling Schizocosa wolf spiders to test for (i) acoustic niche partitioning and (ii) plastic behavioral responses that might reduce the risk of signal interference from substrate-borne noise and conspecific/heterospecific signaling. Two closely related species - S. stridulans and S. uetzi - showed high acoustic niche overlap across space, time, and dominant frequency. Both species show plastic behavioral …

Supportive Supervision And Resiliency Ohio - Resilience Alliance Facilitator Manual, Quality Improvement Center For Workforce Development (Qic-Wd)

Intervention Summaries

No abstract provided.

Supportive Supervision And Resiliency Ohio - Resilience Alliance Participant Handbook, Quality Improvement Center For Workforce Development (Qic-Wd)

Intervention Summaries

No abstract provided.

Soil Carbon And Nitrogen After Eight Years Of Rotational Grazing In The Nebraska Sandhills Meadows, Gandura O. Abagandura, Martha Mamo, W. H. Schacht, Aaron Shropshire, Jerry Volesky

Department of Agronomy and Horticulture: Faculty Publications

Grassland provides many ecosystem services; therefore, sustainable management practices of grassland are crucial for maintaining and enhancing its ecosystem health and resilience. Rotational grazing at a high stocking density (a.k.a., ultrahigh stocking density) is purported to sequester greater amounts of carbon (C) in grassland soils than rotational grazing at low stocking densities. This study was conducted in the Nebraska Sandhills meadows for eight years to evaluate how rotational grazing with different stocking densities can affect soil C and total nitrogen (TN) in bulk soils, soil organic matter fractions, and sequestration rate. The grazing management included a high stocking density with …

Genome-Wide Association And Genomic Prediction For Iron And Zinc Concentration And Iron Bioavailability In A Collection Of Yellow Dry Beans, Paulo Izquierdo, Rie Sadohara, Jason Wiesinger, Raymond Glahn, Carlos Urrea, Karen Cichy

Department of Agronomy and Horticulture: Faculty Publications

Dry bean is a nutrient-dense food targeted in biofortification programs to increase seed iron and zinc levels. The underlying assumption of breeding for higher mineral content is that enhanced iron and zinc levels will deliver health benefits to the consumers of these biofortified foods. This study characterized a diversity panel of 275 genotypes comprising the Yellow Bean Collection (YBC) for seed Fe and Zn concentration, Fe bioavailability (FeBio), and seed yield across 2 years in two field locations. The genetic architecture of each trait was elucidated via genome-wide association studies (GWAS) and the efficacy of genomic prediction (GP) was assessed. …

Habitat Characteristics, Distribution, And Abundance Of Cicindelidia Haemorrhagica (Leconte) (Coleoptera: Cicindelidae) In Yellowstone National Park, K. A. Willemssens, J. L. Bowley, L. Cavallini, E. Oberg, R.K.D. Peterson, Leon G. Higley

School of Natural Resources: Faculty Publications

No abstract provided.

Dynamic Rates Of Freshwater Drum Near The Northern Extent Of Their Range: Evidence Of Environment-Recruitment Relationships, M. Stuart, N. Kludt, M. A. Pegg, F. Montesanto, C. Lyon, C. J. Chance-Ossowski

School of Natural Resources: Faculty Publications

No abstract provided.

Long-Term Croplands Water Productivity In Response To Management And Climate In The Western Us Corn Belt, M. Khorchani, Tala Awada, M. Schmer, V. Jin, G. Birru, S.R.S. Dangal, Andrew E. Suyker, A. Freidenreich

School of Natural Resources: Faculty Publications

No abstract provided.

Exploring Dress, Gender, And Bodily Capital Through Pre- And Protohistoric Funerary Contexts: Case Studies From Southwestern Europe, Francisco B. Gomes, Catarina Costeira, Anna Maria Desiderio, Arianna Esposito, Giacomo Bardelli

Textile Crossroads: Exploring European Clothing, Identity, and Culture across Millennia

While uneven in their scope and reach, studies of dress and dress complements (fibulae, belt buckles, buttons, etc.) have a significant tradition within the broader study of the pre- and protohistory of Mediterranean Europe. Many of these studies, however, have had a strong focus on the typology of the dress complements and ornaments themselves, either as chronological indicators, ethnic markers, or both. In more recent years, however, a shift in research agendas has ushered in the introduction of new perspectives and new ways of thinking about dress and bodily adornment.

This contribution explores one such perspective in particular — namely, …

Tractor Test 2282: John Deere 9rt 590 Diesel, Nebraska Tractor Test Lab

Nebraska Tractor Tests

ABOUT THE TEST REPORT AND USE OF THE DATA The test data contained in this report are a tabulation of the results of a series of tests. Due to the restricted format of these pages, only a limited amount of data and not all of the tractor specifications are included. The full OECD report contains usually about 30 pages of data and specifications. The test data were obtained for each tractor under similar conditions and therefore, provide a means of comparison of performance based on a limited set of reported data. EXPLANATION OF THE TEST PROCEDURES Purpose The purpose of …

Current Challenges And Future Of Agricultural Genomes To Phenomes In The Usa, C. K. Tuggle, J. L. Clarke, B. M. Murdoch, E. Lyons, N. M. Scott, B. Benes, J. D. Campbell, H. Ching, C. L. Daigle, S. D. Choudhury, J.C. M. Dekkers, J.R.R. Dorea, D. S. Erti, B. O. Fragomeni, J. E. Fulton, C. R. Guadagno, D. E. Hagen, A. S. Hess, L. M. Kramer, C. J. Lawrence-Dill, A. E. Lipka, T. Lubberstedt, F. M. Mccarthy, P. S. Schnable

School of Natural Resources: Faculty Publications

No abstract provided.

15-Yr Biomass Production In Semiarid Nebraska Sandhills Grasslands: Part 1—Plant Functional Group Analysis, B. Zhao, B. M. Stephenson, T. Awada, J. Volesky, B. Wardlow

School of Natural Resources: Faculty Publications

No abstract provided.

Implementing Interdisciplinary Sustainability Education With The Food-Energy-Water (Few) Nexus, C. B. Romulo, S. Venkataraman, S. Caplow, S. Ajgaonkar, C. R. Allen, A. Anandhi, S. W. Anderson, C. B. Azzarello, K. Brundiers, E. Blavascunas, J. M. Dauer, D. L. Druckenbrod, E. Fairchild, L. R. Horne, K. Lee, M. Mwale, J. Mischler, E. Pappo, N. S. Patel, N. D. Sintov, C. S. Ramsdell, S. G. Vincent

School of Natural Resources: Faculty Publications

No abstract provided.

Behaviour And Attitudes Of Non‑Resident Water Fowl Hunters In Saskatchewan, M. P. Gruntarod, M. P. Vrtiska, C. J. Chizinski

School of Natural Resources: Faculty Publications

No abstract provided.

Duck Hunters And Difficulty Complying With Harvest Regulations, M. P. Gruntarod, M. P. Vrtiska, C. J. Chizinski, J. N. Duberstein, D. C. Fulton, H. W. Harshaw, A. H. Raedeke, J. Spaeth

School of Natural Resources: Faculty Publications

No abstract provided.

Nitrate Removal By Floating Treatment Wetlands Under Aerated And Unaerated Conditions: Field And Laboratory Results, J. M. Mccoy, M. Chaffee, A. Mittelstet, T. Messer, S. Comfort

School of Natural Resources: Faculty Publications

No abstract provided.

Prioritization Of Research On Drought Assessment In A Changing Climate, J. Lisonbee, B. Parker, E. Fleishman, T. W. Ford, R. K. Bocinsky, B. Fuchs Et Al.

School of Natural Resources: Faculty Publications

No abstract provided.

15-Year Biomass Production In Semiarid Nebraska Sandhills Grasslands: Part 1 – Plant Functional Group Analysis, B. Zhao, M. B. Stephenson, Tala Awada, J. D. Volesky, Brian D. Wardlow, Y. Zhou, Y. Shi

School of Natural Resources: Faculty Publications

No abstract provided.

15-Year Biomass Production In Semiarid Nebraska Sandhills Grasslands: Part 1 – Plant Functional Group Analysis, B. Zhao, M. B. Stephenson, Tala Awada, J. D. Volesky, Brian D. Wardlow, Y. Zhou, Y. Shi

School of Natural Resources: Faculty Publications

No abstract provided.

Topography-Driven Microclimate Gradients Shape Forest Structure, Diversity, And Composition In A Temperate Refugial Forest, B. H. Mcnichol, R. Wang, A. Hefner, C. Helzer, S. M. Mcmahon, S. E. Russo

School of Natural Resources: Faculty Publications

No abstract provided.

Understanding The Values That Inform Regenerative Ranching In The Northern U.S. Great Plains, T. Pape, G. Meredith, D. Sandal, M. F. Kabir, S. Banerjee, C. R. Allen, E. Dennis, M. Stephenson

School of Natural Resources: Faculty Publications

No abstract provided.