Digital Commons Network^™

Overcoming Genetic Paucity Of Camelina Sativa: Possibilities For Interspecific Hybridization Conditioned By The Genus Evolution Pathway, Rostyslav Y. Blume, Ruslan Kalendar, Liang Guo, Edgar B. Cahoon, Yaroslav B. Blume

Center for Plant Science Innovation: Faculty Publications

Camelina or false flax (Camelina sativa) is an emerging oilseed crop and a feedstock for biofuel production. This species is believed to originate from Western Asian and Eastern European regions, where the center of diversity of the Camelina genus is located. Cultivated Camelina species arose via a series of polyploidization events, serving as bottlenecks narrowing genetic diversity of the species. The genetic paucity of C. sativa is foreseen as the most crucial limitation for successful breeding and improvement of this crop. A potential solution to this challenge could be gene introgression from Camelina wild species or from …

Simultaneous 3d Construction And Imaging Of Plant Cells Using Plasmonic Nanoprobe-Assisted Multimodal Nonlinear Optical Microscopy, Kun Liu, Yutian Lei, Dawei Li

Department of Civil and Environmental Engineering: Faculty Publications

Nonlinear optical (NLO) imaging has emerged as a promising plant cell imaging technique due to its large optical penetration, inherent 3D spatial resolution, and reduced photodamage; exogenous nanoprobes are usually needed for nonsignal target cell analysis. Here, we report in vivo, simultaneous 3D labeling and imaging of potato cell structures using plasmonic nanoprobe-assisted multimodal NLO microscopy. Experimental results show that the complete cell structure can be imaged via the combination of second-harmonic generation (SHG) and two-photon luminescence (TPL) when noble metal silver or gold ions are added. In contrast, without the noble metal ion solution, no NLO signals from the …

Simultaneous 3d Construction And Imaging Of Plant Cells Using Plasmonic Nanoprobe-Assisted Multimodal Nonlinear Optical Microscopy, Kun Liu, Yutian Lei, Dawei Li

Department of Civil and Environmental Engineering: Faculty Publications

Nonlinear optical (NLO) imaging has emerged as a promising plant cell imaging technique due to its large optical penetration, inherent 3D spatial resolution, and reduced photodamage; exogenous nanoprobes are usually needed for nonsignal target cell analysis. Here, we report in vivo, simultaneous 3D labeling and imaging of potato cell structures using plasmonic nanoprobe-assisted multimodal NLO microscopy. Experimental results show that the complete cell structure can be imaged via the combination of second-harmonic generation (SHG) and two-photon luminescence (TPL) when noble metal silver or gold ions are added. In contrast, without the noble metal ion solution, no NLO signals from the …

The Localization Of Centromere Protein A Is Conserved Among Tissues, Eleonora Cappelletti, Francesca M. Piras, Lorenzo Sola, Marco Santagostino, Jessica L. Petersen, Rebecca R. Bellone, Carrie J. Finno, Sichong Peng, Ted S. Kalbfleisch, Ernest Bailey, Solomon G. Nergadze, Elena Giulotto

Department of Animal Science: Faculty Publications

Centromeres are epigenetically specified by the histone H3 variant CENP-A. Although mammalian centromeres are typically associated with satellite DNA, we previously demonstrated that the centromere of horse chromosome 11 (ECA11) is completely devoid of satellite DNA. We also showed that the localization of its CENP-A binding domain is not fixed but slides within an about 500 kb region in different individuals, giving rise to positional alleles. These epialleles are inherited as Mendelian traits but their position can move in one generation. It is still unknown whether centromere sliding occurs during meiosis or during development. Here, we first improve the sequence …

Toward A Brighter Future For Entomological Collections, Susan Weller

University of Nebraska State Museum: Entomology Papers

Entomology collections, and natural history collections more generally, are critical research infrastructure. Specimens and metadata such as specimen labels, field notebooks, images, dissections, genetic sequences, and host plant data (to name a few) enable team science to examine biological diversity through time and study the impacts of humans on landscapes and climate (Johnson et al. 2023). However, some do not grasp the connection between drawers of insect specimens and modern science driven by advances in computational methods, molecular biology, and big data. Scientific specimens are key to connecting large-scale research questions within and among entomology and other disciplines.

Social Networks And Violence Victimization And Perpetration Among Youth: A Longitudinal Analysis, Natira Mullet, Emily A. Waterman, Katie M. Edwards, Victoria Banyard, Thomas W. Valente

Nebraska Center for Research on Children, Youth, Families, and Schools: Faculty Publications

Interpersonal violence (IV) is a serious concern for adolescents in the United States that has devastating impacts for individuals and communities. Given the increased importance placed on friendships during adolescence, the purpose of the current study was to examine the extent to which IV experiences cluster within youths' friendship networks. Participants were students (N = 1303) in grades 7th to 10th who completed surveys at the beginning and end of an academic year. Results showed that friends' average perpetration (i.e., the percentage of the friends they nominated who perpetrated IV) was strongly associated with likelihood of individual perpetration at …

Analogue Model Of Rift Linkage And Inversion With Application To The Western Alps, Nicolas Dall’Asta, Yoann Denèle, Vincent Regard, Anne Frayssignes, Guilhem Hoareau, Sylvie Leroy, Thibaut Pires

Department of Earth and Atmospheric Sciences: Faculty Publications

Along-strike segmentation of orogens raises questions because its causes may predate orogeny in relation to structural inheritance. Here we focus on rift/margin linkage domains and their inversion by using analogue models with image analysis to extract the 3D strain field. Extensional models document, depending on the strike-perpendicular offset and the brittle-crust thickness, three types of rift linkage modes: (1) oblique linkage with early T-fault, (2) strongly-oblique linkage with R-fault network and, (3) transfer-linkage with late Y-strike-slip fault. Analogue model of inverted rift basins is used to analyse the misunderstood tectonic evolution of transition zones in the segmented Western Alpine belt.

Conduction Mechanism Switching From Coulomb Blockade To Classical Critical Percolation Behavior In Disordered Nanoparticle Array, Abhijeet Prasad, Jay Min Lim, Ravi Saraf

Department of Chemical and Biomolecular Engineering: Faculty Publications

Large, open-gate transistors made from metal nanoparticle arrays offer possibilities to build new electronic devices, such as sensors. A nanoparticle necklace network (N³) of Au particles from 300 K to cryogenic temperatures exhibit a nonohmic I–V_d behavior, I ≈ (V_d–V_T)^𝜻, where V_T is a conduction gap and 𝜻 is a constant critical exponent. The conduction gap in N³, made from disordered networks of 1D chains of 10 nm diameter Au particles exhibits room temperature (RT) gating. Although the I–V_d behavior at RT is identical to Coulomb blockade, …

U.S. Trade And Investment Restrictions: Laudable But Costly Goals, Christine Mcdaniel

Yeutter Institute International Trade Policy Review

U.S. Commerce Secretary Raimondo’s recent visit to China resulted in the announcement of a new “export control enforcement information exchange” between the United States and China. The laudable goal is to prevent China from using U.S. technology for military purposes against the United States or our allies. An information exchange may be a way to explain things to each other, but the fact remains that the export controls are indeed in place. China represents large revenue streams for three of the largest US chip producers—about 20% for Nvidia, 60% for Qualcomm, and 20-30% for Intel. If these U.S. companies cannot …

Allostatic Load Index Effectively Measures Chronic Stress Status In Zoo-Housed Giraffes, Haley N. Beer, Lisa K. Karr, Trenton C. Schrader, Dustin T. Yates

Department of Animal Science: Faculty Publications

For giraffes, few standardized methods exist for quantifying chronic stress. Allostatic load index is quantified from a panel of multi-system stress biomarkers to estimate cumulative stress. Our objective was to determine whether a panel of biomarkers selected for their role in allostatic load would reflect the number of documented stress events experienced by giraffes. Cortisol, DHEA-S, cholesterol, non-esterified fatty acids (NEFA), and fructosamine were determined in serum samples from zoo-housed giraffes (n = 18). These were correlated with the overall number and frequency of ZIMS-documented stress events experienced prior to blood collection. We also compared giraffes grouped by high vs. …

Optimization Of Removal Of Toluene From Industrial Wastewater Using Rsm Box– Behnken Experimental Design, Dariush Jafari, Morteza Esfandyari, Mehdi Mojahed

Department of Civil and Environmental Engineering: Faculty Publications

The study is concerned with the adsorption of toluene from real wastewater using granular beads of activated carbon. The adsorbent was analyzed before and after the process using Scanning Electron Microscope analysis to characterize its surface characteristics. The adsorption parameters including solution pH, contact time, dosage of adsorbent, temperature and toluene initial concentration were optimized using response surface methodology (RSM) Box-Behnken experimental design to maximize the toluene adsorption. The adsorption capacity of the adsorbent was 298 mg g⁻¹ and the maximum toluene removal was 99.5% which was achieved in the following optimal conditions: pH: 2, 100 min, adsorbent dosage: …

Optimization Of Removal Of Toluene From Industrial Wastewater Using Rsm Box– Behnken Experimental Design, Dariush Jafari, Morteza Esfandyari, Mehdi Mojahed

Department of Civil and Environmental Engineering: Faculty Publications

The study is concerned with the adsorption of toluene from real wastewater using granular beads of activated carbon. The adsorbent was analyzed before and after the process using Scanning Electron Microscope analysis to characterize its surface characteristics. The adsorption parameters including solution pH, contact time, dosage of adsorbent, temperature and toluene initial concentration were optimized using response surface methodology (RSM) Box-Behnken experimental design to maximize the toluene adsorption. The adsorption capacity of the adsorbent was 298 mg g^{− 1} and the maximum toluene removal was 99.5% which was achieved in the following optimal conditions: pH: 2, 100 min, adsorbent …

Genetic Improvement Of Tocotrienol Content Enhances The Oxidative Stability Of Canola Oil, Min Deng, Hao Chen, Wei Zhang, Edgar B. Cahoon, Yongming Zhou, Chunyu Zhang

Center for Plant Science Innovation: Faculty Publications

Background: Tocotrienols and tocopherols, which are synthesized in plastids of plant cells with similar functionalities, comprise vitamin E to serve as a potent lipid-soluble antioxidant in plants. The synthesis of tocopherols involves the condensation of homogentisic acid (HGA) and phytyl diphosphate (PDP) under the catalysis of homogentisate phytyltransferase (HPT). Tocotrienol synthesis is initiated by the condensation of HGA and geranylgeranyl diphosphate (GGDP) mediated by homogentisate geranylgeranyl transferase (HGGT). As one of the most important oil crops, canola seed is regarded as an ideal plant to efficiently improve the production of vitamin E tocochromanols through genetic engineering approaches. However, only …

Animal Board Invited Review: Practical Applications Of Genomic Information In Livestock, D. P. Berry, Matthew L. Spangler

Department of Animal Science: Faculty Publications

Access to high-dimensional genomic information in many livestock species is accelerating. This has been greatly aided not only by continual reductions in genotyping costs but also an expansion in the services available that leverage genomic information to create a greater return-on-investment. Genomic information on individual animals has many uses including (1) parentage verification and discovery, (2) traceability, (3) karyotyping, (4) sex determination, (5) reporting and monitoring of mutations conferring major effects or congenital defects, (6) better estimating inbreeding of individuals and coancestry among individuals, (7) mating advice, (8) determining breed composition, (9) enabling precision management, and (10) genomic evaluations; genomic …

A Naturally Derived Watercress Flower-Based Phenethyl Isothiocyanate-Enriched Extract Induces The Activation Of Intrinsic Apoptosis Via Subcellular Ultrastructural And Ca2+ Efflux Alterations In An In Vitro Model Of Human Malignant Melanoma, Sotiris Kyriakou, Louiza Potamiti, Nikoletta Demosthenous, Tom Amery, Kyle Stewart, Paul G. Winyard, Rodrigo Franco, Aglaia Pappa, Mihalis I. Panayiotidis

School of Veterinary and Biomedical Sciences: Faculty Publications

The aim of the current study was to (i) extract isolated fractions of watercress flowers enriched in polyphenols, phenethyl isothiocyanate and glucosinolates and (ii) characterize the anticancer mode of action of non-lethal, sub-lethal and lethal concentrations of the most potent extract fraction in primary (A375) and metastatic (COLO-679) melanoma cells as well as non-tumorigenic immortalized keratinocyte (HaCaT) cells. Cytotoxicity was assessed via the Alamar Blue assay, whereas ultrastructural alterations in mitochondria and the endoplasmic reticulum were determined via transmission electron microscopy. Mitochondrial membrane depolarization was determined using Mito-MP dye, whereas apoptosis was evaluated through the activation of caspases-3, -8 and …

Two New Absolute Gravity Base Stations Established In Lincoln, Ne, Kris Guthrie, Irina Filina

Transactions of the Nebraska Academy of Sciences and Affiliated Societies

Two new absolute gravity base stations were established on the University of Nebraska-Lincoln City Campus in April 2022. The values were transferred with a relative gravity meter from four existing stations within 238 miles and were later validated by the National Oceanic and Atmospheric Administration. The final values at 22.5 cm above marker height are 980177.781 ± 0.010 mGal at the Lincoln Bell Tower Station and 980178.079 ± 0.010 mGal at the Lincoln Museum station with a gravity gradient of -3.105 µGal/cm. This paper describes the process of transferring gravity values and outlines recommendations for similar projects in the future.

Climate Change Impacts And Adaptation To Permafrost Change In High Mountain Asia: A Comprehensive Review, Prashant Baral, Simon Allen, Jakob F. Steiner, Tika R. Gurung, Graham Mcdowell

Department of Earth and Atmospheric Sciences: Faculty Publications

Changing climatic conditions in High Mountain Asia (HMA), especially regional warming and changing precipitation patterns, have led to notable effects on mountain permafrost. Comprehensive knowledge of mountain permafrost in HMA is mostly limited to the mountains of the Qinghai-Tibetan Plateau, with a strong cluster of research activity related to critical infrastructure providing a basis for related climate adaptation measures. Insights related to the extent and changing characteristics of permafrost in the Hindu Kush Himalaya (HKH), are much more limited. This study provides the first comprehensive review of peer-reviewed journal articles, focused on hydrological, ecological, and geomorphic impacts associated with thawing …

Measurement Of The Top Quark Mass Using A Profile Likelihood Approach With The Lepton + Jets Final States In Proton–Proton Collisions At √ S = 13tev, A. Tumasyan

Department of Physics and Astronomy: Faculty Publications

The mass of the top quark is measured in 36.3 fb⁻¹ of LHC proton–proton collision data collected with the CMS detector at √ s = 13 TeV. The measurement uses a sample of top quark pair candidate events containing one isolated electron or muon and at least four jets in the final state. For each event, the mass is reconstructed from a kinematic fit of the decay products to a top quark pair hypothesis. A profile likelihood method is applied using up to four observables per event to extract the top quark mass. The top quark mass is measured …

Measurement Of The Top Quark Mass Using A Profile Likelihood Approach With The Lepton + Jets Final States In Proton–Proton Collisions At √S = 13tev, A. Tumasyan

Department of Physics and Astronomy: Faculty Publications

The mass of the top quark is measured in 36.3 fb⁻¹ of LHC proton–proton collision data collected with the CMS detector at √ s = 13 TeV. The measurement uses a sample of top quark pair candidate events containing one isolated electron or muon and at least four jets in the final state. For each event, the mass is reconstructed from a kinematic fit of the decay products to a top quark pair hypothesis. A profile likelihood method is applied using up to four observables per event to extract the top quark mass. The top quark mass is measured …

Findings From Seven Years Of Child Welfare Workforce Interventions, Quality Improvement Center For Workforce Development (Qic-Wd)

Other QIC-WD Products

The Quality Improvement Center for WorkforceDevelopment (QIC-WD) operated for seven years and worked with 57 distinct public and tribal child welfare jurisdictions to address workforce issues. We used agency data, an evidence-based management approach, and were supported by site teams that included human resources (HR) and child welfare professionals.

We engaged in in-depth, multi-year projects in eight of the sites, and highlights from those experiences are featured in this short video. A consistent approach to implementation was applied and rigorous evaluation methods were used in those workforce intervention sites, with the goals of improving worker performance, well-being, and retention. Interventions …

Elevated Risk Of Compound Extreme Precipitation Preceded By Extreme Heat Events In The Upper Midwestern United States, Manas Khan, Rabin Bhattarai, Liang Chen

Department of Earth and Atmospheric Sciences: Faculty Publications

Compound extreme events can potentially cause deadlier socio-economic consequences. Although several studies focused on individual extreme climate events, the occurrence of compound extreme events is still not well studied in the upper Midwestern United States. In this study, compound extreme precipitation preceded by extreme hot day events was investigated. Results showed a strong linkage between extreme precipitation events and extreme hot days. A significant increasing trend was noticed mainly in Iowa (10.1%), northern parts of Illinois (5.04%), and Michigan (5.04%). Results also showed a higher intensity of extreme precipitation events preceded by an extremely hot day compared to the intensity …

Aptamer-Based Proteomics Measuring Preoperative Cerebrospinal Fluid Protein Alterations Associated With Postoperative Delirium, Simon T. Dillon, Sarinnapha M. Vasunilashorn, Hasan H. Otu, Long Ngo, Tamara Fong, Xuesong Gu, Michele Cavallari, Alexandra Touroutoglou, Mouhsin Shafi, Sharon K. Inouye, Zhongcong Xie, Edward R. Marcantonio, Towia A. Libermann

Department of Electrical and Computer Engineering: Faculty Publications

Delirium is a common postoperative complication among older patients with many adverse outcomes. Due to a lack of validated biomarkers, prediction and monitoring of delirium by biological testing is not currently feasible. Circulating proteins in cerebrospinal fluid (CSF) may reflect biological processes causing delirium. Our goal was to discover and investigate candidate protein biomarkers in preoperative CSF that were associated with the development of postoperative delirium in older surgical patients. We employed a nested case–control study design coupled with high multiplex affinity proteomics analysis to measure 1305 proteins in preoperative CSF. Twenty-four matched delirium cases and non-delirium controls were selected …

Salmonella Enterica Induces Biogeography-Specific Changes In The Gut Microbiome Of Pigs, Joao Carlos Gomes-Neto, Natasha Pavlovikj, Samantha A. Naberhaus, Bailey Arruda, Andrew K. Benson, Amanda J. Kreuder

Department of Food Science and Technology: Faculty Publications

Swine are a major reservoir of an array of zoonotic Salmonella enterica subsp. enterica lineage I serovars including Derby, Typhimurium, and 4,[5],12:i:- (a.k.a. Monophasic Typhimurium). In this study, we assessed the gastrointestinal (GI) microbiome composition of pigs in different intestinal compartments and the feces following infection with specific zoonotic serovars of S. enterica (S. Derby, S. Monophasic, and S. Typhimurium). 16S rRNA based microbiome analysis was performed to assess for GI microbiome changes in terms of diversity (alpha and beta), community structure and volatility, and specific taxa alterations across GI biogeography (small and large intestine, feces) …

Broken Tails In Holstein Dairy Cattle: A Cross-Sectional Study, Hannah E. Olsen, Karly N. Anderson, Katherine C. Creutzinger, Kurt D. Vogel

Department of Animal Science: Faculty Publications

Dairy cows are regularly handled when moved to the milking parlor and during other routine procedures. Low-stress handling methods are important in avoiding negative welfare states for dairy cattle. Tail twisting is used by some handlers to prompt cattle movement. However, when used inappropriately with excessive force, tail twisting can lead to a broken tail. The aim of this cross-sectional study was to determine cow-level factors that may be associated with the prevalence of broken tails in dairy cattle. A subset of 229 Holstein dairy cows (68 primiparous and 161 multiparous) at a single dairy were assessed for broken tails …

Revealing Gene Regulation-Based Neural Network Computing In Bacteria, Samitha S. Somathilaka, Sasitharan Balasubramaniam, Daniel P. Martins, Xu Li

School of Computing: Faculty Publications

Bacteria are known to interpret a range of external molecular signals that are crucial for sensing environmental conditions and adapting their behaviors accordingly. These external signals are processed through a multitude of signaling transduction networks that include the gene regulatory network (GRN). From close observation, the GRN resembles and exhibits structural and functional properties that are similar to artificial neural networks. An in-depth analysis of gene expression dynamics further provides a new viewpoint of characterizing the inherited computing properties underlying the GRN of bacteria despite being non-neuronal organisms. In this study, we introduce a model to quantify the gene-to-gene interaction …

Resilin Distribution And Abundance In Apis Mellifera Across Biological Age Classes And Castes, Audrey Anderson, Noah Keime, Chandler Fong, Andrew Kraemer, Carol Fassbinder-Orth

Department of Agricultural and Biological Systems Engineering: Faculty Publications

The presence of resilin, an elastomeric protein, in insect vein joints provides the flexible, passive deformations that are crucial to flapping flight. This study investigated the resilin gene expression and autofluorescence dynamics among Apis mellifera (honey bee) worker age classes and drone honey bees. Resilin gene expression was determined via ddPCR on whole honey bees and resilin autofluorescence was measured in the 1m-cu, 2m-cu, Cu-V, and Cu2-V joints on the forewing and the Cu-V joint of the hindwing. Resilin gene expression varied significantly with age, with resilin activity being highest in the pupae. Autofluorescence of the 1m-cu and the Cu-V …

Human Alcohol-Microbiota Mice Have Increased Susceptibility To Bacterial Pneumonia, Kelly C. Cunningham, Deandra R. Smith, Daniel Villageliú, Christi M. Ellis, Amanda E. Ramer-Tait, Jeffrey D. Price, Todd A. Wyatt, Daren L. Knoell, Patricia E. Molina, David A. Welsh, Derrick R. Samuelson

Department of Food Science and Technology: Faculty Publications

Preclinical studies have shown that chronic alcohol abuse leads to alterations in the gastrointestinal microbiota that are associated with behavior changes, physiological alterations, and immunological effects. However, such studies have been limited in their ability to evaluate the direct effects of alcohol-associated dysbiosis. To address this, we developed a humanized alcoholmicrobiota mouse model to systematically evaluate the immunological effects of chronic alcohol abuse mediated by intestinal dysbiosis. Germ-free mice were colonized with human fecal microbiota from individuals with high and low Alcohol Use Disorders Identification Test (AUDIT) scores and bred to produce human alcohol-associated microbiota or human control-microbiota F1 progenies. …

Graduate Student Award Winners In Educational Psychology: What Made Them Successful?, Kenneth A. Kiewra, Saima Hasnin, Jared Soundy, Priya Karimuddanahalli Premkumar, Chris Labenz

Department of Educational Psychology: Faculty Publications

Much is known about the factors that make some educational psychologists highly productive. Beginning nearly 25 years ago, Kiewra and colleagues began a series of six qualitative investigations to uncover the keys to scholarly success in educational psychology. The initial study (Kiewra & Creswell, 2000) investigated Richard Anderson, Richard Mayer, and Michael Pressley, who were ranked as the top scholars in a survey of educational psychologists. The second study (Patterson- Hazley & Kiewra, 2013), more than a decade later, investigated productive scholars Patricia Alexander, Richard Mayer, Dale Schunk, and Barry Zimmerman who were ranked as the top scholars in a …

Stomatal Opening Efficiency Is Controlled By Cell Wall Organization In Arabidopsis Thaliana, Sedighe Keynia, Leila Jaafar, You Zhou, Charles T. Anderson, Joseph A. Turner

Department of Mechanical and Materials Engineering: Faculty Publications

Stomatal function in plants is regulated by the nanoscale architecture of the cell wall and turgor pressure, which together control stomatal pore size to facilitate gas exchange and photosynthesis. The mechanical properties of the cell wall and cell geometry are critical determinants of stomatal dynamics. However, the specific biomechanical functions of wall constituents, for example, cellulose and pectins, and their impact on the work required to open or close the stomatal pore are unclear. Here, we use nanoindentation in normal and lateral directions, computational modeling, and microscopic imaging of cells from the model plant Arabidopsis thaliana to investigate the precise …

What Is The Priestley–Taylor Wet-Surface Evaporation Parameter? Testing Four Hypotheses, Richard D. Crago, Jozsef Szilagyi, Russell J. Qualls

School of Natural Resources: Faculty Publications

This study compares four different hypotheses regarding the nature of the Priestley–Taylor parameter α. They are as follows:

1. α is a universal constant.
2. The Bowen ratio (H/LE, where H is the sensible heat flux, and LE is the latent heat flux) for equilibrium (i.e., saturated air column near the surface) evaporation is a constant times the Bowen ratio at minimal advection (Andreas et al., 2013).
3. Minimal advection over a wet surface corresponds to a particular relative humidity value.
4. α is a constant fraction of the difference from the minimum value of 1 to the maximum value of α …