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Virtual Fencing In Livestock Management: Effects On Beef Cattle Stress And Enhancing Wildlife Conservation, Kaitlyn E. Dozler Jul 2024

Virtual Fencing In Livestock Management: Effects On Beef Cattle Stress And Enhancing Wildlife Conservation, Kaitlyn E. Dozler

School of Natural Resources: Dissertations, Theses, and Student Research

I examined the effects of virtual fencing on beef cattle stress and how implementing the technology can aid wildlife habitat conservation at the University of Nebraska Gudmundsen Sandhills laboratory (GSL) near Whitman, NE. First, I examined how virtual fencing influences acute heart rate changes in beef cattle fitted with the virtual fencing collars. No significant difference in the average heart rate of a cow (P > 0.05) was observed between the 30 min control and virtual fenced periods for both trial dates. Second, I examined the influence of topography on wildlife species richness and presence at GSL using wildlife camera …


Spatial Ecology Of Mountain Lions Throughout Their Distribution In California, Kyle D. Dougherty Jul 2024

Spatial Ecology Of Mountain Lions Throughout Their Distribution In California, Kyle D. Dougherty

School of Natural Resources: Dissertations, Theses, and Student Research

Mountain lions (Puma concolor) have been studied extensively throughout their wide geographic distribution in the Western Hemisphere, but most research has been conducted at relatively small spatial scales. We used GPS telemetry data contributed by researchers in 16 study areas throughout California to advance understanding of mountain lion spatial ecology. First, we investigated multi-scale resource selection of mountain lions relative to spatially varying risk of intentional human-caused mortality associated with livestock depredation management. We show that individuals that proactively avoided mortality risk when selecting home ranges were able to relax their avoidance of risk when making resource selection …


My Ai Companion: An Examination Of The Removal Of Erotic Role Play From Replika Through User Discussion On Reddit, Chelsee M. Allen Jul 2024

My Ai Companion: An Examination Of The Removal Of Erotic Role Play From Replika Through User Discussion On Reddit, Chelsee M. Allen

Department of Sociology: Dissertations, Theses, and Student Research

The development of artificial intelligence (AI) software has expanded rapidly in recent years, and thus has emerged the importance of exploring human relationships with AI chatbots. Replika, an app which uses AI to mimic human conversation, removed a function called Erotic Role Play (ERP) that allowed for sexual conversation with users’ customizable chatbots in February of 2023. This exploratory qualitative study examines the aftermath of ERP’s removal through an analysis of user interactions on Reddit. Five overarching themes emerged through the analysis of top posts to a Replika-specific subreddit, encompassing topics around mental health, stigma, coping, sex work and gendered …


French Interwar Popular Romance And Ideals Of Femininity: A Literary And Historical Study Of Magali, Kelly Kamrath Jul 2024

French Interwar Popular Romance And Ideals Of Femininity: A Literary And Historical Study Of Magali, Kelly Kamrath

Department of Modern Languages and Literatures: Dissertations, Theses, and Student Research

Against a background of political turmoil, economic crises, and cultural upsets from both within and abroad, France between the two World Wars was home to a flourishing romance novel industry. At first glance lighthearted love stories seem simple and frivolous in comparison to the canonical writings of the time, such as those of Simone de Beauvoir and Jean-Paul Sartre. However, this literature was far from art for art’s sake. The romance novels of interwar France, written half a century before the scholarship of romance fiction would truly begin, have yet to receive the attention they deserve. During the interwar period, …


Integrating Remote Sensing And Machine Learning To Determine Past, Current And Future Crop Water Use From The Nubian Sandstone Aquifer System, Moaz Ishag Jul 2024

Integrating Remote Sensing And Machine Learning To Determine Past, Current And Future Crop Water Use From The Nubian Sandstone Aquifer System, Moaz Ishag

Department of Agricultural and Biological Systems Engineering: Dissertations, Theses, and Student Research

The agriculture sector is a significant consumer of water, and sustainable water use begins with monitoring irrigated land. Delineating irrigated land supports decision-makers and promotes the sustainable use of this crucial resource. This study focuses on the Nubian Sandstone Aquifer System (NSAS), the largest aquifers in the world, which spans Egypt, Sudan, Libya, and Chad. The study aims to: 1) quantify the increase in irrigated hectares (both pivot and non-pivot) from 2000-2001 to 2023-2024; 2) identify major irrigated crop types and their water requirements; and 3) quantify groundwater crop water use from the NSAS using remote sensing via the Google …


Structural Analysis Of Dj-1 Glyoxalase Activity By Mix-And-Inject Serial Synchrotron Crystallography, Coleman Dolamore Jul 2024

Structural Analysis Of Dj-1 Glyoxalase Activity By Mix-And-Inject Serial Synchrotron Crystallography, Coleman Dolamore

Department of Biochemistry: Dissertations, Theses, and Student Research

Observing enzyme structures while they are catalyzing a reaction has been a major goal of both enzymology and structural biology for decades. With the advent of time-resolved serial X-ray crystallography using X-ray free electron lasers (XFELs) and synchrotron sources, enzyme reactions can be monitored in real time in crystallo. Here, we use two different approaches to study related enzymes involved in methylglyoxal detoxification. For human DJ-1, we used pink beam mix-and-inject serial crystallography (MISC) at the Advanced Photon Source (BioCARS 14-ID) to probe the controversial mechanism of DJ-1’s action on methylglyoxal by using serial Laue diffraction. The high flux …


Data-Driven Drug Repurposing For Immune System-Related Diseases, Sabyasachi Mohanty Jul 2024

Data-Driven Drug Repurposing For Immune System-Related Diseases, Sabyasachi Mohanty

Department of Biochemistry: Dissertations, Theses, and Student Research

In this research, we explore a data-driven approach for drug repurposing to enhance the immunomodulatory effect by integrating pattern-based search and genome-scale metabolic modeling. This research helps to find a solution to the preexisting problems of drug discovery which includes expenses and a high amount of time consumption. By leveraging the pre-existing data of the approved drugs to identify the major metabolic pathway changes, we can find new drugs with similar effects with less off-target effects.

The focus on the immune system modulating drugs is due to the high prevalence of immune system-involved diseases and the growing demand for effective …


Enhancing Water Sustainability In North Africa: Literature Review And Synthesis Of Current Knowledge Gaps In Sudan, Osman M. A. Adam, Aaron R. Mittelstet, Derek M. Heeren, Troy E. Gilmore Jul 2024

Enhancing Water Sustainability In North Africa: Literature Review And Synthesis Of Current Knowledge Gaps In Sudan, Osman M. A. Adam, Aaron R. Mittelstet, Derek M. Heeren, Troy E. Gilmore

Department of Agricultural and Biological Systems Engineering: Presentations and White Papers

This study delves into the critical role of groundwater in addressing global water challenges, with a focus on the Nubian Sandstone Aquifer System (NSAS) in North Africa. Groundwater constitutes a source of potable water, irrigation, and industrial use, especially in arid regions where surface water is limited. We analyzed the status of water quantity, withdrawals, recharge, and geological characteristics in the NSAS, specifically in Sudan, Egypt, Libya, and Chad. Though the NSAS is largely an untapped resource, we evaluated various scenarios to determine the quantity of cropland that can be sustainably irrigated. The NSAS is located in an arid region, …


Assessing Soil Properties For Optimized Irrigation Development In Sudan, Northern Africa, Suhib Hamid, Derek M. Heeren, Aaron R. Mittelstet, Saleh Taghvaeian, Randall S. Ritzema Jul 2024

Assessing Soil Properties For Optimized Irrigation Development In Sudan, Northern Africa, Suhib Hamid, Derek M. Heeren, Aaron R. Mittelstet, Saleh Taghvaeian, Randall S. Ritzema

Department of Agricultural and Biological Systems Engineering: Presentations and White Papers

Sustainable irrigation methods maximize agricultural productivity in Northern African countries like Sudan. This project evaluates soil properties to develop optimized irrigation strategies for Sudan's arid and semi-arid climate. Assessing soil characteristics such as texture, infiltration rates, and nutrient content informs the selection of modern irrigation systems. Data from 3,192 locations, collected using GPS and ring infiltrometers for infiltration rates, alongside laboratory analyses for soil properties, were employed. A ranking system determined suitable irrigation systems for specific soil classes, considering factors like slope, wind, crop tolerance, available water capacity (AWC), drainage, germination, capital cost, labor cost, maintenance, and evapotranspiration (ET). The …


Dynamic Metabolic Flux Analysis Amidst Data Variability: Sphingolipid Biosynthesis Case Study In Arabidopsis Thaliana Cell Cultures, Abraham Boluwatife Osinuga Jul 2024

Dynamic Metabolic Flux Analysis Amidst Data Variability: Sphingolipid Biosynthesis Case Study In Arabidopsis Thaliana Cell Cultures, Abraham Boluwatife Osinuga

Department of Chemical and Biomolecular Engineering: Dissertations, Theses, and Student Research

Sphingolipids are pivotal for plant development and stress responses. Growing interest has been directed towards fully comprehending the regulatory mechanisms of the sphingolipid pathway. In this study, we explore its de novo biosynthesis and homeostasis in Arabidopsis thaliana cell cultures, shedding light on fundamental metabolic mechanisms. Employing 15N isotope labeling and quantitative dynamic modeling approach, we obtained data with notable variations and developed a regularized and constraint-based Dynamic Metabolic Flux Analysis (r-DMFA) framework to predict metabolic shifts due to enzymatic changes. Our analysis revealed key enzymes such as sphingoid-base hydroxylase (SBH) and long-chain-base kinase …


Archaeoentomology: Arthropods And Humans, Current Works In Archaeoentomology, Paz Vigil Jul 2024

Archaeoentomology: Arthropods And Humans, Current Works In Archaeoentomology, Paz Vigil

Department of Entomology: Distance Master of Science Projects

This paper presents a brief literature review, exploring human and insect interaction, and how such research aids the ongoing construction of the archaeological and entomological record. This field, a combined subset of both entomology and archaeology, is both complex and rich as well as young and still growing. This literature review is not meant to be an exhaustive or comprehensive exploration of this subject, but it will serve as part of a foundation for those interested in the topic or wanting to conduct further exploratory study in this field. The intent is to highlight the practical applications of Archaeoentomology, using …


Exploring Gen Z’S Identity Formation And Its Influence On Consumption Of Pop Culture And Entertainment Merchandise, Melisa Annette Spilinek Jul 2024

Exploring Gen Z’S Identity Formation And Its Influence On Consumption Of Pop Culture And Entertainment Merchandise, Melisa Annette Spilinek

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

Fandoms have grown in size and influence, becoming a complex and dynamic cultural phenomenon that richly impacts the consumption of media and consumer behavior. The capitalistic United States with its diverse and media-savvy population, is a prime destination to study fan studies, particularly among young adults who are a crucial component of fandom influence.

The internet has grown to be a major component of the fan experience, an integral part of fandom culture. Online communities provide avenues for Generation Z to connect with others who share similar struggles, challenges, and interests, highlighting the importance of digital platofrms on the evolution …


Torus Surgery, Fibrations, Multisections, And Spun 4-Manifolds, Nicholas Paul Meyer Jul 2024

Torus Surgery, Fibrations, Multisections, And Spun 4-Manifolds, Nicholas Paul Meyer

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

A compact n-manifold X is fibered if it is a fiber bundle where the fiber F and base space B are manifolds. Fibered manifolds are particularly nice, as they are essentially classified by their monodromy maps. Two common examples of 4-dimensional fibered manifolds are surface bundles over surfaces and 3-manifold bundles over the circle.

The main focus of this dissertation is to investigate fibered 4-manifolds whose boundaries are the 3-torus and how these manifolds glue together to give new closed, fibered 4-manifolds. In particular, suppose W is diffeomorphic to S1 × EY (K) where Y …


Singer’S Guide To Hak Jun Yoon’S Selected Art Songs, Sunmin Cha Jul 2024

Singer’S Guide To Hak Jun Yoon’S Selected Art Songs, Sunmin Cha

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

Composer Hak Jun Yoon is at the forefront of popularizing Korean art songs through the combination of artistry and mass appeal, positioning him as one of the representative composers of contemporary Korea. Recently, Yoon’s songs gained significant popularity after being featured on the Korean vocal competition TV program “Phantom Singer,” playing a major role in sparking public interest in Korean art songs in general. This document aims to widen that circle to more classical singers by analyzing poetic and musical elements in four of Yoon’s representative songs: “On the Way to You,” “A Flower Blooms Alone,” “Lingering Scent,” and “The …


A Case Study Of Mathematical Instructional Leadership, Theresa Haack Jul 2024

A Case Study Of Mathematical Instructional Leadership, Theresa Haack

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

This dissertation looks at the impact of instructional leadership in mathematics teaching on teachers at various stages in their careers. Using a case study methodology, the researcher explores the nuanced needs and effective strategies for beginning, mid-career, and established teachers. Peer instructional leaders provided data that involved a mix of qualitative methods, including surveys, interviews, and questionnaires, targeting five assistant principals selected randomly from diverse school environments. The study identifies common themes and differences in instructional approaches, highlighting how strategies vary and overlap across different career stages. Analysis gives insights into the practical application of leadership in educational settings, emphasizing …


Electronic Properties Of Group-Iii Nitride Semiconductors And Device Structures Probed By Thz Optical Hall, Nerijus Armakavicius, Philipp Kühne, Alexis Papamichail, Hengfang Zhang, Sean Knight, Axel Persson, Vallery Stanishev, Jr-Tai Chen, Plamen Paskov, Mathias Schubert, Vanya Darakchieva Jun 2024

Electronic Properties Of Group-Iii Nitride Semiconductors And Device Structures Probed By Thz Optical Hall, Nerijus Armakavicius, Philipp Kühne, Alexis Papamichail, Hengfang Zhang, Sean Knight, Axel Persson, Vallery Stanishev, Jr-Tai Chen, Plamen Paskov, Mathias Schubert, Vanya Darakchieva

Department of Electrical and Computer Engineering: Faculty Publications

Group-III nitrides have transformed solid-state lighting and are strategically positioned to revolutionize high-power and high-frequency electronics. To drive this development forward, a deep understanding of fundamental material properties, such as charge carrier behavior, is essential and can also unveil new and unforeseen applications. This underscores the necessity for novel characterization tools to study group-III nitride materials and devices. The optical Hall effect (OHE) emerges as a contactless method for exploring the transport and electronic properties of semiconductor materials, simultaneously offering insights into their dielectric function. This nondestructive technique employs spectroscopic ellipsometry at long wavelengths in the presence of a magnetic …


Exploring The Associations Of Afterschool Science Participation And Friendships With Science Identities, Patricia Wonch Hill, Grace M. Kelly, Julia Mcquillan, Jorge Ledesma, Miranda Melson, G. Robin Gauthier Jun 2024

Exploring The Associations Of Afterschool Science Participation And Friendships With Science Identities, Patricia Wonch Hill, Grace M. Kelly, Julia Mcquillan, Jorge Ledesma, Miranda Melson, G. Robin Gauthier

Worlds of Connections Resources

Building on previous research that demonstrates the association of youth experiences in afterschool science and higher science identities, this paper presents a network study of 421 middle school students that examines afterschool science participation, friendship ties, and science identities. Participation in afterschool science clubs is associated with higher science identity, but the mechanisms and order of causality are unclear. Youth form friendships inside and outside of school, and peers may influence participation in afterschool activities, as empirical research on friendships shows that they are associated with youth interests. These peer interactions also have the potential to shape identity development during …


Evaluation Of An Autonomous Acoustic Surveying Technique For Grassland Bird Communities In Nebraska, Grace E. Schuster, Leroy J. Walston, Andrew R. Little Jun 2024

Evaluation Of An Autonomous Acoustic Surveying Technique For Grassland Bird Communities In Nebraska, Grace E. Schuster, Leroy J. Walston, Andrew R. Little

School of Natural Resources: Faculty Publications

Monitoring trends in wildlife communities is integral to making informed land management decisions and applying conservation strategies. Birds inhabit most niches in every environment and because of this they are widely accepted as an indicator species for environmental health. Traditionally, point counts are the common method to survey bird populations, however, passive acoustic monitoring approaches using autonomous recording units have been shown to be cost-effective alternatives to point count surveys. Advancements in automatic acoustic classification technologies, such as BirdNET, can aid in these efforts by quickly processing large volumes of acoustic recordings to identify bird species. While the utility of …


Propelling Nebraska's Wind Power, Peter F. Biedenweg Jun 2024

Propelling Nebraska's Wind Power, Peter F. Biedenweg

Nebraska Law Bulletin (Selected Issues)

In 2014 a study for the Nebraska Power Review Board noted: "In the near term, renewable generation developers in Nebraska face competitive disadvantages that include: (a) lower financial incentives from the state; (b) lower wholesale power prices due to transmission congestion; (c) [and] the perception of a more burdensome permitting and regulatory process." Today, these issues persist. State financial incentives are still lacking, transmission congestion has only worsened, and zoning and permitting laws remain burdensome. Through the recommendations of this paper, however, it may be possible to begin addressing these issues.

Nebraska can see more wind development through the Legislature …


Nebraska Farm Real Estate Market Highlights 2023-2024, Jim Jansen, Jeff Stokes Jun 2024

Nebraska Farm Real Estate Market Highlights 2023-2024, Jim Jansen, Jeff Stokes

Nebraska Farm Real Estate Reports

For the fifth consecutive year, the all-land average value in Nebraska rose for the year ending February 1, 2024, averaging approximately 5% higher than the prior year. Figure 2 summarizes these figures and trends along with the percent changes over the preceding year’s all-land average for the eight districts in the state.

The statewide all-land average value for the year ending February 1, 2024, averaged $4,015 per acre, or about a 5% ($180 per acre) increase from the prior year’s value of $3,835 per acre. Rates of increase were the highest in the North and Northeast Districts, as these areas …


Perspectives On Computational Modeling Of Biological Systems And The Significance Of The Sysmod Community, Bhanwar Lal Puniya, Meghna Verma, Chiara Damiani, Shaimaa Bakr, Andreas Dräger Jun 2024

Perspectives On Computational Modeling Of Biological Systems And The Significance Of The Sysmod Community, Bhanwar Lal Puniya, Meghna Verma, Chiara Damiani, Shaimaa Bakr, Andreas Dräger

Department of Biochemistry: Faculty Publications

Motivation: In recent years, applying computational modeling to systems biology has caused a substantial surge in both discovery and practical applications and a significant shift in our understanding of the complexity inherent in biological systems.

Results: In this perspective article, we briefly overview computational modeling in biology, highlighting recent advancements such as multiscale modeling due to the omics revolution, single-cell technology, and integration of artificial intelligence and machine learning approaches. We also discuss the primary challenges faced: integration, standardization, model complexity, scalability, and interdisciplinary collaboration. Lastly, we highlight the contribution made by the Computational Modeling of Biological Systems …


Puberty Classifications In Beef Heifers Are Moderately To Highly Heritable And Associated With Candidate Genes Related To Cyclicity And Timing Of Puberty, Melanie K. Hess, Anteneh Mersha, Sadie S. Ference, Sarah R. Nafziger, Jessica A. Keane, Anna M. Fuller, Scott G. Kurz, Courtney M. Sutton, Matthew L. Spangler, Jessica Lynn Petersen, Andrea S. Cupp Jun 2024

Puberty Classifications In Beef Heifers Are Moderately To Highly Heritable And Associated With Candidate Genes Related To Cyclicity And Timing Of Puberty, Melanie K. Hess, Anteneh Mersha, Sadie S. Ference, Sarah R. Nafziger, Jessica A. Keane, Anna M. Fuller, Scott G. Kurz, Courtney M. Sutton, Matthew L. Spangler, Jessica Lynn Petersen, Andrea S. Cupp

Department of Animal Science: Faculty Publications

Introduction: Pubertal attainment is critical to reproductive longevity in heifers. Previously, four heifer pubertal classifications were identified according to attainment of blood plasma progesterone concentrations > 1 ng/ml: 1) Early; 2) Typical; 3) Start-Stop; and 4) Non-Cycling. Early and Typical heifers initiated and maintained cyclicity, Start-Stop started and then stopped cyclicity and Non- Cycling never initiated cyclicity. Start-Stop heifers segregated into Start-Stop- Discontinuous (SSD) or Start-Stop-Start (SSS), with SSD having similar phenotypes to Non-Cycling and SSS to Typical heifers. We hypothesized that these pubertal classifications are heritable, and loci associated with pubertal classifications could be identified by genome wide association …


Computable Species Descriptions And Nanopublications: Applying Ontology-Based Technologies To Dung Beetles (Coleoptera, Scarabaeinae), Giulio Montanaro, James P. Balhoff, Jennifer C. Girón, Max Söderholm, Sergei Tarasov Jun 2024

Computable Species Descriptions And Nanopublications: Applying Ontology-Based Technologies To Dung Beetles (Coleoptera, Scarabaeinae), Giulio Montanaro, James P. Balhoff, Jennifer C. Girón, Max Söderholm, Sergei Tarasov

Department of Agricultural Leadership, Education, and Communication: Faculty Publications

Taxonomy has long struggled with analysing vast amounts of phenotypic data due to computational and accessibility challenges. Ontology-based technologies provide a framework for modelling semantic phenotypes that are understandable by computers and compliant with FAIR principles. In this paper, we explore the use of Phenoscript, an emerging language designed for creating semantic phenotypes, to produce computable species descriptions. Our case study centers on the application of this approach to dung beetles (Coleoptera, Scarabaeinae).


Production Of The Siderophore Lysochelin In Rich Media Through Maltose-Promoted High-Density Growth Of Lysobacter Sp. 3655, Fang Zhang, Jia Liu, Lin Jiang, Yongbiao Zheng, Lingjun Yu, Liangcheng Du Jun 2024

Production Of The Siderophore Lysochelin In Rich Media Through Maltose-Promoted High-Density Growth Of Lysobacter Sp. 3655, Fang Zhang, Jia Liu, Lin Jiang, Yongbiao Zheng, Lingjun Yu, Liangcheng Du

Department of Chemistry: Faculty Publications

Siderophores are produced by bacteria in iron-restricted conditions. However, we found maltose could induce the biosynthesis of the siderophore lysochelin in Lysobacter sp. 3655 in rich media that are not compatible with siderophore production. Maltose markedly promoted cell growth, with over 300% increase in cell density (OD600) when LB medium was added with maltose (LBM). While lysochelin was not detectable when OD600 in LBM was below 5.0, the siderophore was clearly produced when OD600 reached 7.5 and dramatically increased when OD600 was 15.0. Coincidently, the transcription of lysochelin biosynthesis genes was remarkably enhanced following the …


Concepts In Animal Parasitology, Scott L. Gardner, Sue Ann Gardner Jun 2024

Concepts In Animal Parasitology, Scott L. Gardner, Sue Ann Gardner

Zea E-Books Collection

Note that this version of the textbook has been superceded by the one updated in 2026, available at the UNL Pressbooks site.

The hard copy version that is for sale at lulu.com is the old version.

"This is a textbook covering concepts in animal parasitology. It is meant to be used by students, teachers, professors, researchers, and members of the public who are interested in learning about animal parasite biology, systematics, taxonomy, zoogeography, and ecology. The primary intended audience is upper-level undergraduate or graduate university students who have knowledge of basic biology and, particularly, basic animal biology. (863 pages, …


Tsunami Exposure And Mental Health Consequences: Protective Role Of Cultural Coping Strategies, Thulitha Wickrama, Michael J. Merten, K. A.S. Wickrama, Amanda Terrell Jun 2024

Tsunami Exposure And Mental Health Consequences: Protective Role Of Cultural Coping Strategies, Thulitha Wickrama, Michael J. Merten, K. A.S. Wickrama, Amanda Terrell

Department of Child, Youth, and Family Studies: Faculty Publications

There is a knowledge gap regarding the link between disaster exposure and adolescent mental health problems in developing countries. This study examines the case of Sri Lanka to investigate (a) the immediate and long-term mental health impact of the 2004 tsunami disaster on adolescents and (b) the potential moderating effects of unique cultural and family practices that prevail in Sri Lanka. This study used a random sample of 160 adolescents (ages 12–19) and their mothers who were exposed to the tsunami disaster while living in a southern Sri Lankan village and provided prospective data immediately after the disaster (2005) and …


Malnutrition Alters Protein Expression Of Kndy Neuropeptides In The Arcuate Nucleus Of Mature Ewes, Jennifer F. Thorson, Ligia D. Prezotto Jun 2024

Malnutrition Alters Protein Expression Of Kndy Neuropeptides In The Arcuate Nucleus Of Mature Ewes, Jennifer F. Thorson, Ligia D. Prezotto

Department of Animal Science: Faculty Publications

The neuropeptides kisspeptin, neurokinin B, and dynorphin A are imperative for the pulsatile secretion of gonadotropin-releasing hormone and luteinizing hormone to ultimately regulate reproductive cyclicity. A population of neurons co-expressing these neuropeptides, KNDy neurons, within the arcuate nucleus of the hypothalamus (ARC) are positioned to integrate energy status from afferent neuronal and glial cells. We hypothesized that KNDyexpressing neurons in the ARC of mature ewes are influenced by energy balance. To test this hypothesis, ovary-intact, mature ewes were fed to lose, maintain, or gain body weight and hypothalamic tissue harvested during the luteal phase of the estrous cycle. Fluorescent, multiplex …


Cellmarkerpipe: Cell Marker Identification And Evaluation Pipeline In Single Cell Transcriptomes, Yinglu Jia, Pengchong Ma, Qiuming Yao Jun 2024

Cellmarkerpipe: Cell Marker Identification And Evaluation Pipeline In Single Cell Transcriptomes, Yinglu Jia, Pengchong Ma, Qiuming Yao

School of Computing: Faculty Publications

Assessing marker genes from all cell clusters can be time-consuming and lack systematic strategy. Streamlining this process through a unified computational platform that automates identification and benchmarking will greatly enhance efficiency and ensure a fair evaluation. We therefore developed a novel computational platform, cellMarkerPipe (https:// github. com/ yao- labor atory/ cellM arker Pipe), for automated cell-type specific marker gene identification from scRNA-seq data, coupled with comprehensive evaluation schema. CellMarkerPipe adaptively wraps around a collection of commonly used and state-of-the-art tools, including Seurat, COSG, SC3, SCMarker, COMET, and scGeneFit. From rigorously testing across diverse samples, we ascertain SCMarker’s overall reliable performance …


Violent Conflict Drives World Hunger And Food Insecurity, E. Wesley F. Peterson Jun 2024

Violent Conflict Drives World Hunger And Food Insecurity, E. Wesley F. Peterson

Cornhusker Economics

The World Food Program (WFP) with support from the United States and other countries works to provide food to those in need as a result of conflict, adverse climate effects, poverty, and government mismanagement. It is limited in what it can do, however, by budget constraints and rising food prices. On top of the financial issues, the delivery of food assistance is particularly complicated in areas of conflict where warring adversaries often hijack food supplies intended for the other side. While it is illegal under International Humanitarian Law to use starvation as a weapon of war, Dzerowicz (2023) has identified …


Topological Regression As An Interpretable And Efficient Tool For Quantitative Structureactivity Relationship Modeling, Ruibo Zhang, Daniel Nolte, Cesar Sanchez-Villalobos, Souparno Ghosh, Ranadip Pal Jun 2024

Topological Regression As An Interpretable And Efficient Tool For Quantitative Structureactivity Relationship Modeling, Ruibo Zhang, Daniel Nolte, Cesar Sanchez-Villalobos, Souparno Ghosh, Ranadip Pal

Department of Statistics: Faculty Publications

Quantitative structure-activity relationship (QSAR)modeling is a powerful tool for drug discovery, yet the lack of interpretability of commonly used QSAR models hinders their application inmolecular design.We propose a similaritybased regression framework, topological regression (TR), that offers a statistically grounded, computationally fast, and interpretable technique to predict drug responses. We compare the predictive performance of TR on 530 ChEMBL human target activity datasets against the predictive performance of deep-learning-based QSAR models. Our results suggest that our sparse TR model can achieve equal, if not better, performance than the deep learningbased QSAR models and provide better intuitive interpretation by extracting an approximate …