Digital Commons Network^™

Greater Aperture Counteracts Effects Of Reduced Stomatal Density On Water Use Efficiency: A Case Study On Sugarcane And Meta-Analysis, Daniel Lunn, Baskaran Kannan, Amandine Germon, Alistair Leverett, Thomas E. Clemente, Fredy Altpeter, Andrew D.B. Leakey

Department of Agronomy and Horticulture: Faculty Publications

Stomata regulate CO₂ and water vapor exchange between leaves and the atmosphere. Stomata are a target for engineering to improve crop intrinsic water use efficiency (iWUE). One example is by expressing genes that lower stomatal density (SD) and reduce stomatal conductance (g_sw). However, the quantitative relationship between reduced SD, g_sw, and the mechanisms underlying it is poorly understood. We addressed this knowledge gap using low-SD sugarcane (Saccharum spp. hybrid) as a case study alongside a meta-analysis of data from 10 species. Transgenic expression of EPIDERMAL PATTERNING FACTOR 2 from Sorghum bicolor (SbEPF2) in sugarcane …

Development And Evaluation Of An Expedited System For Creation Of Single Walled Carbon Nanotube Platforms, Ivon Acosta Ramirez, Omer Sadak, Wali Sohail, Xi Huang, Yongfeng Lu, Nicole M. Iverson

Department of Electrical and Computer Engineering: Faculty Publications

Single-walled carbon nanotubes (SWNT) have a strong and stable near-infrared (nIR) fluorescence that can be used to selectively detect target analytes, even at the single molecule level, through changes in either their fluorescence intensity or emission peak wavelength. SWNTs have been employed as NIR optical sensors for detecting a variety of analytes. However, high costs, long fabrication times, and poor distributions limit the current methods for immobilizing SWNT sensors on solid substrates. Recently, our group reported a protocol for SWNT immobilization with high fluorescence yield, longevity, fluorescence distribution, and sensor response, unfortunately this process takes 5 days to complete. Herein …

Assessment Of Genetic Diversity And Population Structure Of U.S. Polypay Sheep From Breed Origins To Future Genomic Selection, Carrie S. Wilson, Jessica Lynn Petersen, Luiz F. Brito, Brad A. Freking, Sara M. Nilson, J. Bret Taylor, Thomas W. Murphy, Ronald M. Lewis

Department of Animal Science: Faculty Publications

Knowledge of past and present genetic diversity within a breed is critical for the design and optimization of breeding programs as well as the development of strategies for the conservation of genetic resources. The Polypay sheep breed was developed at the U.S. Sheep Experiment Station (USSES) in 1968 with the goal of improving productivity in Western U.S. range flocks. It has since flourished in the more intensively managed production systems throughout the U.S. The genetic diversity of the breed has yet to be documented. Therefore, the primary objective of this study was to perform a comprehensive evaluation of the genetic …

Segundo Congreso Internacional De Iconografía Precolombina, 2023. Actas, Victòria Solanilla Demestre

Zea E-Books Collection

El objetivo de este II Congreso fue, como en el anterior, abrir un espacio de reflexión propositiva y multidisciplinaria para que, a partir de análisis de objetos plásticos u otras manifestaciones concretas, así como de documentos escritos, se pudiera replantear la importancia de la imagen y de la interpretación iconográfica como fuente de conocimiento de las culturas precolombinas. Es el momento de seguir aceptando nuevos enfoques pluridisciplinares o si se quiere más “humanísticos” que nos den una visión lo más completa posible, a través de la iconografía, del pasado precolombino tan rico en imágenes y símbolos.

Contribuciones de Luz Adriana …

Optimized Gadolinium-Do3a Loading In Raftpolymerized Copolymers For Superior Mr Imaging Of Aging Blood–Brain Barrier, Hunter A. Miller, Aaron Priester, Evan T. Curtis, Krista Hilmas, Ashleigh Abbott, Forrest Kievit, Anthony J. Covertine

Department of Agricultural and Biological Systems Engineering: Faculty Publications

The development of gadolinium-based contrast agents (GBCAs) has been pivotal in advancing magnetic resonance imaging (MRI), offering enhanced soft tissue contrast without ionizing radiation exposure. Despite their widespread clinical use, the need for improved GBCAs has led to innovations in ligand chemistry and polymer science. We report a novel approach using methacrylate-functionalized DO3A ligands to synthesize a series of copolymers through direct reversible addition-fragmentation chain transfer (RAFT) polymerization. This technique enables precise control over the gadolinium content within the polymers, circumventing the need for subsequent conjugation and purification steps, and facilitates the addition of other components such as targeting ligands. …

Revisiting Fr13a For Submergence Tolerance: Beyond The Sub1a Gene, Waseem Hussain, Mahender Anumalla, Abdelbagi M. Ismail, Harkamal Walia, Vistas Kumar Singh, Ajay Kohli, Sankalp Bhosale, Hans Bhardwaj

Department of Agronomy and Horticulture: Faculty Publications

Rice landrace FR13A is the original donor of the SUB1A gene. FR13A harbors hidden genetic variation that surpasses the submergence tolerance level of SUB1A. Here, we provide an overview of the unique features of FR13A associated with exceptional recovery ability that have been overlooked since the significant discovery of the SUB1 locus. We present a detailed overview of how to underpin the new genes associated with the recovery ability seen in FR13A. Finally, we propose a unique approach to develop rice cultivars that surpass submergence tolerance beyond the SUB1 locus from FR13A.

Lymphoid Tissues Contribute To Plasma Viral Clonotypes Early After Antiretroviral Therapy Interruption In Siv-Infected Rhesus Macaques, Antonio Solis-Leal, Nongthombam Boby, Suvadip Mallick, Yilun Cheng, Fei Wu, Grey De La Torre, Jason Dufour, Xavier Alvarez, Vinay Shivanna, Yaozhong Liu, Christine M. Fennessey, Jeffrey D. Lifson, Qingsheng Li, Brandon F. Keele, Binhua Ling

School of Biological Sciences: Faculty Publications

The rebound-competent viral reservoir, composed of a virus that is able to persist during antiretroviral therapy (ART) and mediate reactivation of systemic viral replication and rebound viremia after ART interruption (ATI), remains the biggest obstacle to treating HIV infection. A better understanding of the cellular and tissue origins and the dynamics of viral populations that initiate rebound upon ATI could help develop therapeutic strategies for reducing the rebound-competent viral reservoir. In this study, barcoded simian immunodeficiency virus (SIV), SIVmac239M, was used to infect rhesus macaques to enable monitoring of viral barcode clonotypes contributing to virus detectable in plasma after ATI. …

Evaluation Of The Capacity And Effective Angle Of Thalamic Damage For 2 Commercially Available Captive Bolt Tool Types On Cadaver Heads From Sows >200 Kg Body Weight, Karly N. Anderson, Ruth Woiwode, Ashlynn A. Kirk, Jennifer Berger, Arquimides A. Reyes, Perle E. Zhitnitskiy, Kurt D. Vogel

Department of Animal Science: Faculty Publications

This study evaluated the ability of 2 penetrating captive bolt (PCB) types (PISTOL, INLINE) to reach and disrupt the thalamus when applied in 2 placements (FRONTAL, BEHIND EAR) to chilled cadaver heads (N = 60) from sows >200 kg. Heads were randomly distributed across 6 treatments (n = 10): FRONTAL-INLINE, FRONTAL-PISTOL, FRONTAL-NO SHOT, BEHIND EAR-INLINE, BEHIND EAR-PISTOL, and BEHIND EAR-NO SHOT. The FRONTAL shot was placed 3.5 cm superior to the optic orbits at the midline; the BEHIND EAR shot was placed directly caudal to the pinna of the ear on the same plane as the eyes and targeting the …

Resources In Northeast Nebraska's Waste Could Power The Future, Reese Vanosdel, Emma Kurtz, Tyler Knaak

Project Energy Nebraska: Student Projects

Environmental Engagement in the Community projects, summer 2024

A poster titled Resources in Northeast Nebraska's Waste Could Power the Future for clients City of Norfolk, Nebraska (Steven Rames) and City of Wayne, Nebraska (Wes Blecke and Sandy Brown).

Advisor: David Gosselin

Second Language Learning In Older Adults Modulates Stroop Task Performance And Brain Activation, Douglas H. Schultz, Alison Gansemer, Kiley Allgood, Mariah Gentz, Lauren Secilmis, Zoha Deldar, Cary R. Savage, Ladan Ghazi Saidi

Center for Brain, Biology, and Behavior: Faculty Publications

Introduction: Numerous studies have highlighted cognitive benefits in lifelong bilinguals during aging, manifesting as superior performance on cognitive tasks compared to monolingual counterparts. Yet, the cognitive impacts of acquiring a new language in older adulthood remain unexplored. In this study, we assessed both behavioral and fMRI responses during a Stroop task in older adults, pre- and post language-learning intervention.

Methods: A group of 41 participants (age: 60–80) from a predominantly monolingual environment underwent a four-month online language course, selecting a new language of their preference. This intervention mandated engagement for 90 minutes a day, five days a week. …

Strengthening Linkages Between Health Care And Public Health: A Novel Framework For Public Health Action In Health Care Settings To Improve Cardiovascular Disease Risk, Candice Fryda, Liz Gebhart-Morgan, Mindy Anderson-Knott, Nicole Roseberry, Jamie Hahn, Kelli Niemeier, Jennifer Rutt, Debra Miller

UNL Office of Research and Innovation, Social/Behavioral Sciences Research Consortium

The Chronic Disease Prevention and Control Program (CDPCP) at the Nebraska Department of Health and Human Services developed a novel public health framework and tools to translate public health knowledge, grant work, and terminology to a health care audience in order to inform clinical practice changes in the management of hyperlipidemia and hypertension. The CDPCP piloted the tools with 2 accountable care organizations that included 19 clinics and then funded 9 independent clinics. The project sought to empower clinics to design and implement interventions for reducing high blood pressure and high blood cholesterol focused on populations disproportionately at risk for …

A Two-Part Project, Phil Warburton

Department of Entomology: Distance Master of Science Projects

1) an analysis of the constraints to the use of entomological observations collected by citizen scientists for research

The online database, iNaturalist, is transforming observational entomology with a massive and valuable database of photographed insect sightings in their environment. Increasingly, iNaturalist is becoming a rich source of scientific data for research purposes. However, iNaturalist faces a hidden but potentially serious crisis-in-the making. The phenomenal growth threatens to outstrip the capacity of expert insect identifiers.

As the database grows rapidly, the rate of identification of insect observations to the “Research Grade” standard is in decline. Furthermore, the number of expert identifiers …

Water Current, Volume 56, No. 2, Summer 2024

Water Current Newsletter

No abstract provided.

Evaluating A Precision Sprayer For Detecting Weeds And Spraying Herbicides In Real Time (Spot Spray) For Weed Management In Corn And Soybean, Adam Eric Leise

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Herbicide sprayers are becoming larger and more efficient in applications in crop fields. A precise and efficient herbicide application is advantageous in effective weed control. New precision sprayers were introduced in hopes of reducing the amount of herbicide applied. Greeneye Technology and John Deere have developed precision sprayers for the detection of weeds in crop fields, which come equipped with a dual tank system that allows for two different herbicides to be separated from tank to tip. The objectives of this study were to evaluate the broadleaf and grass weed control efficacy of spot spray (SS) technology when applied in …

Blanket Statements, Issue 164, Summer 2024

Blanket Statements: News Publication of the American Quilt Study Group

Blanket Statements, Issue 164, Summer 2024

Utilization Of Probiotics To Compete With Clostridioides Difficile For Nutrient-Niches In A Variety Of In Vitro Contexts, April Elizabeth Johnson

Department of Food Science and Technology: Dissertations, Theses, and Student Research

Clostridium difficile is a major threat to public health and primarily affects at risk groups such as elderly, immunocompromised individuals, and individuals on antibiotics. Many decades of research have been conducted to figure out ways to exclude or reduce C. difficile colonization and toxin production. Two major proposed mechanisms for colonization resistance are production of secondary bile salts and competition for nutrients.

In Chapter 2, we found that antibiotic treatment disrupted bile salt metabolism increasing the levels of the primary bile salt cholate and decreasing the levels of deoxycholate, a secondary bile salt. However, bile salts were not required …

Design And Evaluation Of An Esa-Based Method Of Ensemble Subsetting For A Wofs (Warn On Forecast-Like System), Daniel J. Butler

Department of Earth and Atmospheric Sciences: Dissertations, Theses, and Student Research

Forecasting severe thunderstorm environments in the southeastern United States can be challenging due to mesoscale heterogeneities such as shortwave troughs, pre-existing airmass boundaries, cold fronts aloft, low-level jets, dry air intrusions, and mesoscale lows. To combat these challenges, ensemble sensitivity analysis (ESA) may be applied to a Warn-on-Forecast (WOF)-like ensemble to improve forecasts of severe convection through ensemble weighting and subsetting. Ensemble-based weighting and subsetting uses ensemble members that most accurately represent the thunderstorm environment in areas of mesoscale heterogeneity. This study creates and evaluates the ensemble-based weighting and subsetting in four cases of severe thunderstorm occurrence. The open parameter …

Examining Dietary Disparities And Associated Factors Among Young Children Across Diverse Racial, Ethnic And Immigrant Households In Nebraska, Naveta Bhatti

College of Education and Human Sciences: Dissertations, Theses, and Student Research

Early childhood is a crucial stage for developing eating behaviors which in turn plays a major role in determining health outcomes. Assessing children’s diet plays a crucial role in tackling the obesity epidemic. One of the ways to assess children’s dietary intake is by evaluating their dietary quality. Dietary quality refers to the overall healthfulness and nutritional value of an individual's diet, considering the combination of foods consumed over time. Research has consistently demonstrated that a high-quality diet is associated with a reduced risk of several health issues, such as all-cause mortality, cardiovascular disease incidence or mortality, cancer incidence or …

Development Of A Rule-Based Monitoring System For Autonomous Heavy Equipment Safety, Amirpooya Shirazi

Department of Construction Engineering and Management: Dissertations, Theses, and Student Research

Roadway construction work zones are constantly exposed to interactions among construction equipment, workers, and vehicles. Furthermore, ensuring safety in these areas is considered a challenging task due to the complexity of the environment. As shown in the rising trend of fatal accidents in roadway work zones, current OSHA regulations in construction safety are insufficient in effectively detecting unsafe situations and mitigating the risks. Furthermore, best practices, such as internal traffic control planning (ITCP), exhibit critical limitations requiring continuous monitoring of active work zones as well as adjustments to the site coordination plans due to the dynamic nature of work zone …

Party Over Democracy? When Do Americans Stand By Democratic Principles, Zenat Ahmed

Department of Political Science: Dissertations, Theses, and Student Research

Studies show that people support their party’s representatives even when their behavior violates fundamental democratic principles for partisan gain. We test the effects of two interventions- each randomly and independently assigned—to investigate the conditions under which people will prioritize democracy over party goals. In the first intervention, we explicitly point out how an in-party politician’s behavior (e.g., banning public gatherings of their opponents) violates a core democratic principle (e.g., free assembly). The second intervention is a question-order manipulation to test if participants are less tolerant of politicians’ antidemocratic behavior when support for democratic principles is reported first. We find that …

Parenting Identity And Parental Mental Health In Chinese Parents In Mainland China, Zhenqiao Yang

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

In the modern context of mainland China, parents of young children face unique parenting stressors, making parental mental health during the early stages of parenting a significant public concern. Increasing awareness of the role of self-concepts in mental health underscores the importance of exploring the relationships between parenting identity concepts, parenting stress, parental mental health, and familial factors. This study aims to examine the relationship between parenting identity and parental mental health, specifically depression and anxiety, in the context of early childhood parenting stress. Data were collected from 456 parents at four daycare centers and public spaces in an Eastern …

Exploring Producers' Willingness To Pay For Ecosystem Services: Three Essays On Soil Health, Location Preferences, And Cover Crop Adoption, Kaouter Essakkat

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

This dissertation comprises three essays: one assessing Midwest producers' willingness-to-pay (WTP) for soil health-enhancing ecosystem services (ES), another examining the impact of policy implementation locations on WTP, and a third identifying factors influencing producers' adoption of cover crops (CC).

The first essay pioneers the understanding of agricultural producers’ valuation of soil health-associated ES amid growing concerns of soil degradation due to agricultural intensification. Using a discrete choice experiment, this study assesses producers’ WTP for a policy that incentivizes ES provision, specifically improved water quality, carbon sequestration, and enhanced crop yield. Surveying producers across Iowa, Kansas, and Nebraska, our results show …

Assessment Of Foam Decontamination In Tomato Washing Process, Basim M. Alohali

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

The work of the dissertation investigated the efficacy of foam-based decontamination treatments to enhance microbial safety in Roma tomatoes, specifically targeting natural microflora and Salmonella contamination. Growing concerns over foodborne illnesses linked to fresh tomatoes and the limitations of conventional decontamination techniques, such as organic matter built-up and limited microbial reduction, are addressed. The primary objectives were to characterize the physiochemical properties of foams formed using various concentrations of Tween 20 (T20) and sodium dodecyl sulfate (SDS) with and without the addition of sodium hypochlorite (NaOCl) and peracetic acid (PAA), compare the efficacy of foam and traditional dump tank (DT) …

Development Of A Durable And Low-Dose Universal Vaccine Against Swine Influenza A Virus, Erika Petro-Turnquist

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

Swine Influenza A Virus (IAV-S) imposes a significant burden on the pork industry and poses a great threat to public health due to its zoonotic potential. Susceptibility to human, avian, and swine influenza make pigs the ultimate mixing vessels for reassorted influenza A virus strains. Current vaccination strategies induce short-lived and strain-specific responses against IAV-S. Further, the substantial genetic diversity of IAV-S necessitates consistent revaccination and updating of commercial vaccines. Computational platforms have recently emerged as a promising strategy to develop new-age vaccines against infectious diseases in livestock animals. Here, we describe a computationally derived set of vaccine immunogens, called …

Estranged Temporality: How Time Tells Stories In Science Fiction, Phillip H. Howells

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

According to Darko Suvin in his influential critical treatise, Metamorphoses of Science Fiction, estrangement in Science Fiction (SF) gives authors the ability to build worlds related to but distant from our own using specific metaphors. This dissertation takes up this term and applies it to the fulcrum of time in SF as a method of creating possible futures and imaginative pasts in order to illuminate the realities of the present. The realities of the present are congruent with the material circumstances of the past, and this can be seen in the kinds of SF worlds built by members of …

Spatial Ecology Of Mountain Lions Throughout Their Distribution In California, Kyle Duncan Dougherty

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

Mountain lions (Puma concolor) have been studied extensively throughout their wide geographic distribution in the Western Hemisphere, but most research has been conducted at relatively small spatial scales. We used GPS telemetry data contributed by researchers in 16 study areas throughout California to advance understanding of mountain lion spatial ecology. First, we investigated multi-scale resource selection of mountain lions relative to spatially varying risk of intentional human-caused mortality associated with livestock depredation management. We show that individuals that proactively avoided mortality risk when selecting home ranges were able to relax their avoidance of risk when making resource selection …

Genetically Modified Bovine Milk-Derived Small Extracellular Vesicles In Drug Delivery, Alice Kah Hui Ngu

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

Small extracellular vesicles (SEVs, “exosomes”) are natural nanoparticles that can be endogenously synthesized and also obtained from dietary sources, such as bovine milk SEVs (mEVs). mEVs have been extensively studied and discovered with contributive features that potentially play a role in drug delivery and other therapeutic purposes, such as diagnostic tools. Current studies have shown that mEVs have been used preclinically to carry in targeting drugs, nucleotides, biomolecules, and labels to target metabolic diseases and cancers. Extensive studies have focused on further modifying SEVs to enhance their versatility in pharmaceutical use. In this dissertation, we exhibited one of the ways …

Laser 3d Printing Of Diamond-Based Composite Materials For Thermal Management Applications, Nada Kraiem

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

With the trend towards miniaturization of electrical equipment and the constant increase in power density in semiconductor devices, efficient heat management has become a major concern for researchers. Indeed, this technological evolution imposes increasingly strict constraints in terms of thermal dissipation, necessitating innovative solutions to ensure better durability and reliability of components. In this context, the use of composite materials offering high thermal conductivity (TC) and low coefficient of thermal expansion (CTE) compared to pure metals has become essential to address overheating issues in electronic components.

The utilization of advanced materials such as diamond (D), with exceptional TC and hardness …

Risk Factors For Online And In-Person Stalking, Intrusive Harassment, And Violence Within A Community Sample, Elisha J. C. Chan

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

Stalking and intrusive harassment (SIH) and stalking violence are prevalent and serious public health concerns associated with significant physical, psychological, financial, and social consequences. The growing proliferation of technology and the internet has only exacerbated the problem as SIH behaviors can be easily perpetrated via electronic means. Given its prevalence and negative impact, it is imperative to investigate the risk factors for online and in-person SIH and stalking violence to better predict and mitigate its detrimental effects. The current study therefore aimed to 1) examine the descriptive characteristics associated with online and in-person SIH and stalking violence; and 2) identify …

Population Demography, Occupancy, And Connectivity Of Invasive Bigheaded Carp In The Platte River, Nebraska And Its Tributaries, Blake W. Logan

School of Natural Resources: Dissertations, Theses, and Student Research

Silver Carp Hypophthalmichthys molitrix and Bighead Carp H. nobilis, collectively referred to as bigheaded carp, are invasive species in the United States of America that have expanded their range throughout the Missouri River basin in the last three decades. It may be desirable to develop management plans directed at the monitoring, control, or removal of bigheaded carp within the Missouri River basin. However, a knowledge gap exists regarding bigheaded carp ecology in Missouri River tributaries, which may be important for the development of management plans. As such, this study characterized bigheaded carp population demographics, occupancy, and connectivity in …