Digital Commons Network^™

Challenges In Gluten Analysis: A Comparison Of Four Commercial Sandwich Elisa Kits, Plaimein Amnuaycheewa, Lynn Niemann, Richard E. Goodman, Joseph L. Baumert, Steve L. Taylor

Department of Food Science and Technology: Faculty Publications

Gluten is composed of prolamin and glutelin proteins from several related grains. Because these proteins are not present in identical ratios in the various grains and because they have some differences in sequence, the ability to accurately quantify the overall amount of gluten in various food matrices to support gluten-free labeling is difficult. Four sandwich ELISAs (the R-Biopharm AG R5 RIDASCREEN®, the Neogen Veratox® R5, the Romer Labs AgraQuant® G12, and the Morinaga Wheat kits) were evaluated for their performance to quantify gluten concentrations in various foods and ingredients. The Morinaga and AgraQuant® G12 tests yielded results comparable to the …

Tissue Losses And Metabolic Adaptations Both Contribute To The Reduction In Resting Metabolic Rate Following Weight Loss, Alexandra Martin, Darius Fox, Chaise A. Murphy, Hande Hofmann, Karsten Koehler

Department of Nutrition and Health Sciences: Faculty Publications

OBJECTIVE: To characterize the contributions of the loss of energy-expending tissues and metabolic adaptations to the reduction in resting metabolic rate (RMR) following weight loss.

METHODS: A secondary analysis was conducted on data from the Comprehensive Assessment of Long-term Effects of Reducing Intake of Energy study. Changes in RMR, body composition, and metabolic hormones were examined over 12 months of calorie restriction in 109 individuals. The contribution of tissue losses to the decline in RMR was determined by weighing changes in the size of energy-expending tissues and organs (skeletal muscle, adipose tissue, bone, brain, inner organs, residual mass) …

A Review Of The Impacts Of Invasive Wild Pigs On Native Vertebrates, Matthew T. Mcdonough, Stephen S. Ditchkoff, Mark D. Smith, Kurt C. Vercauteren

United States Department of Agriculture Wildlife Services: Staff Publications

The wild pig (Sus scrofa) is a successful invasive species that has become well established outside of its native range in Eurasia. The invasive wild pig is the result of released or escaped domesticated livestock becoming feral, or Eurasian boar introduced for hunting purposes. The global spread of wild pigs has recently been exacerbated in some areas, such as the USA, by anthropogenically assisted dispersal. Once established in novel ecosystems, wild pigs have the potential to have significant negative impacts on the ecosystem, and the scientific literature is replete with examples. It is generally accepted that wild pigs …

Role Of Specialized Pro-Resolving Mediators In Reducing Neuroinflammation In Neurodegenerative Disorders, Jana Ponce, Arzu Ulu, Corrine Hanson, Erin Cameron-Smith, Jenna Wuebker, John Bertoni, Alfred Fisher, Ka-Chun Siu, Vivien Marmelat, Jiri Adamec, Danish Bhatti

Department of Biochemistry: Faculty Publications

Alzheimer’s disease (AD) and Parkinson’s disease (PD) are neurodegenerative disorders that affect millions of individuals worldwide. As incidence of these conditions increases with age, there will undoubtedly be an increased prevalence of cases in the near future. Neuroinflammation is a hallmark in the development and progression of neurodegenerative diseases and prevention or resolution of chronic neuroinflammation may represent a novel approach to treatment. The present review highlights the potential of the anti-inflammatory and pro-resolving effects of polyunsaturated fatty acid (PUFA)- derived mediators (Specialized Pro-resolving Mediators—SPM) in neurodegenerative disorders. PUFA-derived SPM are biosynthesized in response to chemicals produced from acute inflammatory …

What Kigali’S Open‑Air Markets Reveal About Achieving Food And Nutrition Security: The Role Of African Indigenous Crops, Eugene Baraka, Mary S. Willis, Brice A. Ishimwe

Department of Nutrition and Health Sciences: Faculty Publications

Background: Household dietary diversity in Rwanda remains low and significantly contributes to the double burden of malnutrition. Rwanda has one of the highest under five stunting rates globally, and malnutrition remains one of the most pressing public health issues; therefore, factors that shape food and nutrition security are of utmost concern. Globally, the variety of foods available in open-air markets has been shown to affect dietary diversity. Furthermore, the consumption of indigenous foods can contribute to a diverse diet and improve nutrition status. At present, there are limited data on foods available for purchase in open-air markets in Africa. …

The Crucial Role Of Sestrins In Oxidative Stress Response And Disease: An Analysis Of Phosphorylation Mutation S110a In Hsesn2, Julianne Fay

Honors Program: Senior Projects (Public)

Sestrins are highly conserved proteins which are induced under environmentally stressful conditions to adjust the cell’s response to such disturbances. This protein possesses two domains critical for its dual function in reducing reactive oxygen species (ROS) and regulating the mTORC1 complex. Sestrins may use diverse transcription factors, such as Nrf2, peroxiredoxin reductase activity, and a catalytic cysteine to regenerate oxidized species; however this was confirmed to be through a mechanism likely different than AhpD, for which there was distance sequence similarity. Furthermore, Sestrins are important regulators of TORC1 and AMPK, which are crucial energy sensors able to control cellular metabolism …

Regional Minimums In The U.S. Beef Complex, Elliott James Dennis, Bradley Lubben

Center for Agricultural Profitability

This report shows how the currently proposed policies differ; shows how these policies have aligned with historical market behavior; provides alternative specifications to regional minimums; and suggests policy alternatives to regional minimums.

The main purpose of this report is to show how current and potential alternative specifications of regional minimums would have historically aligned with observed market behavior. However, the fundamental question in the debate of the validity and effectiveness of regional minimums first rests on whether robust price discovery has historically occurred over time and within each USDA-AMS region. If there has been a lack of price discovery during …

Evaluation Of Hermetic Storage Bags For The Preservation Of Yellow Maize In Poultry Farms In Dormaa Ahenkro, Ghana, Bismark Opoku, Enoch Adjei Osekre, George Opit, Augustine Bosomtwe, Georgina Bingham

Department of Entomology: Faculty Publications

Using low-quality maize, resulting from insect pests and fungal attack, for formulating feed reduces chicken performance. This study evaluated the effectiveness of hermetic storage bags to keep insect pest and mycotoxin levels in check in yellow maize. The study was conducted in storehouses at three poultry farms in Dormaa Ahenkro, Bono Region, Ghana. The experiment was set up in a Randomized Complete Block Design with ZeroFly®Hermetic (ZFH), Purdue Improved Crop Storage (PICS), and Polypropylene (PP) bags as treatments. In each treatment, twelve 50 kg samples of untreated maize were each put in 100 kg capacity bags. Two bags in each …

Water Insecurity, Water Borrowing, And Psychosocial Stress Among Daasanach Pastoralists In Northern Kenya, Leslie Ford, Hilary J. Bethancourt, Zane Swanson, Rosemary Nzunza, Amber Wutich, Alexandra Brewis, Sera Young, David Almeida, Matthew J. Douglass, Emmanuel K. Ndiema, David R. Braun, Herman Pontzer, Asher Y. Rosinger

School of Natural Resources: Faculty Publications

This article quantifies Daasanach water insecurity experiences in Northern Kenya, examines how water insecurity is associated with water borrowing and psychosocial stress, and evaluates if water borrowing mitigates the stress from water insecurity. Of 133 households interviewed in 7 communities, 95% were water insecure and 74.4% borrowed water three or more times in the prior month. Regression analyses demonstrate water borrowing frequency moderates the relationship between water insecurity and psychosocial stress. Only those who rarely or never borrowed water reported greater stress with higher water insecurity. The coping mechanism of water borrowing may help blunt water insecurity-related stress.

Effects Of Antimicrobial Addition On Lipid Oxidation Of Rendered Chicken Fat, Janak Dhakal, Dalton Holt, Charles G. Aldrich, Carl Knueven

Department of Food Science and Technology: Faculty Publications

This study evaluated the effects of antimicrobial acidulant addition on lipid oxidation of rendered chicken fat. Chicken fat was untreated (control) or treated with either sodium bisulfate (SBS) or lactic acid (LA) at 0.5% w/w and incubated for 6 wk at 40 °C. Peroxide value (PV), p-anisidine value (AV), and free fatty acid (FFA) levels were measured at days 0 (D0), 1(D1), 3 (D3), 5 (D5), and 7 (D7), and weeks 2 (W2), 3 (W3), 4 (W4), 5 (W5), and 6 (W6). The FFA level of untreated-control fat was ~7% and remained consistent throughout the incubation until W6 (~8.5%; P …

Assessment Of Bacterial Inoculant Delivery Methods For Cereal Crops, Yen Ning Chai, Stephanie Futrell, Daniel P. Schachtman

Department of Agronomy and Horticulture: Faculty Publications

Despite growing evidence that plant growth-promoting bacteria can be used to improve crop vigor, a comparison of the different methods of delivery to determine which is optimal has not been published. An optimal inoculation method ensures that the inoculant colonizes the host plant so that its potential for plant growth-promotion is fully evaluated. The objective of this study was to compare the efficacy of three seed coating methods, seedling priming, and soil drench for delivering three bacterial inoculants to the sorghum rhizosphere and root endosphere. The methods were compared across multiple time points under axenic conditions and colonization efficiency was …

Evaluation Of Bioactive Properties Of Lipophilic Fractions Of Edible And Non-Edible Parts Of Nasturtium Officinale (Watercress) In A Model Of Human Malignant Melanoma Cells, Sotiris Kyriakou, Venetia Tragkola, Heba Alghol, Ioannis Anestopoulos, Tom Amery, Kyle Stewart, Paul G. Winyard, Dimitrios T. Trafalis, Rodrigo Franco, Aglaia Pappa, Mihalis I. Panayiotidis

School of Veterinary and Biomedical Sciences: Faculty Publications

Watercress is an enriched source of phenethyl isothiocyanate (PEITC), among other phytochemicals, with an antioxidant capacity. The aim of this study was to (i) chemically characterize and (ii) biologically evaluate the profile of the main health-promoting compounds contained in edible (i.e., mixture of leaves and lateral buds) and non-edible (i.e., stems) parts of watercress in an in vitro model of malignant melanoma consisting of human malignant melanoma (A375), non-melanoma (A431) and keratinocyte (HaCaT) cells. The extraction of the main constituents of watercress was performed by subjecting the freeze-dried edible and non-edible samples through different extraction protocols, whereas their concentration was …

Preliminary Evidence That Lectins In Infant Soy Formula Apparently Bind Bovine Milk Exosomes And Prevent Their Absorption In Healthy Adults, Ezra Mutai, Alice Kah Hui Ngu, Janos Zempleni

Department of Nutrition and Health Sciences: Faculty Publications

Background: Milk exosomes and their microRNA (miR) cargos are bioavailable. The content of exosomes and miRs is negligible in infant formulas compared to human milk, and dietary depletion of exosomes led to changes in bacterial communities and impaired gut health in juvenile mice. Adverse effects of formula feeding may be compounded by using soy formulas due to exosome binding by abundant lectins in that matrix. The purpose of this study was to assess the bioavailability of milk exosomes and their miR cargos added to soy formula in adults, as well as the potential role of soy lectins in exosome …

Genetic Parameters, Heterosis, And Breed Effects For Body Condition Score And Mature Cow Weight In Beef Cattle, André Mauric F. Ribeiro, Leticia P. Sanglard, Warren M. Snelling, R. Mark Thallman, Larry A. Kuehn, Matthew L. Spangler

Department of Animal Science: Faculty Publications

Understanding the genetic relationship between mature cow weight (MWT) and body condition score (BCS) is useful to implement selection programs focused on cow efficiency. The objectives of this study were to estimate genetic parameters, heterosis, and breed effects for MWT and BCS. In total, 25,035 and 24,522 overlapping records were available for MWT and BCS on 6,138 and 6,131 cows, respectively, from the Germplasm Evaluation program, a crossbred beef population at the U.S. Meat Animal Research Center. Pedigree was available for 48,013 individuals. Univariate animal models were used to estimate heritabilities for each trait by parity. …

Corn Nitrogen Nutrition Index Prediction Improved By Integrating Genetic, Environmental, And Management Factors With Active Canopy Sensing Using Machine Learning, Dan Li, Yuxin Miao, Curtis J. Ransom, Gregory Mac Bean, Newell R. Kitchen, Fabián G. Fernández, John E. Sawyer, James J. Camberato, Paul R. Carter, Richard B. Ferguson, David W. Franzen, Carrie A. M. Laboski, Emerson D. Nafziger, John F. Shanahan

Department of Agronomy and Horticulture: Faculty Publications

Accurate nitrogen (N) diagnosis early in the growing season across diverse soil, weather, and management conditions is challenging. Strategies using multi-source data are hypothesized to perform significantly better than approaches using crop sensing information alone. The objective of this study was to evaluate, across diverse environments, the potential for integrating genetic (e.g., comparative relative maturity and growing degree units to key developmental growth stages), environmental (e.g., soil and weather), and management (e.g., seeding rate, irrigation, previous crop, and preplant N rate) information with active canopy sensor data for improved corn N nutrition index (NNI) prediction using machine learning methods. Thirteen …

Applications Of Stretching Technique And Time Window Effects On Ultrasonic Velocity Monitoring In Concrete, Bibo Zhong, Jinying Zhu

Department of Civil and Environmental Engineering: Faculty Publications

Coda wave interferometry (CWI) has been used to measure the relative wave-velocity change (dV/V) caused by small changes in materials. This study uses the stretching processing technique which has been used for CWI analysis to investigate velocity changes of direct longitudinal (P) wave, direct shear (S) wave, and coda wave in concrete by choosing different time windows of ultrasonic signals. It is found that the obtained wave-velocity change depends on the time window position, because the relative contribution of P wave and S wave is different in each signal window. This paper presents three experimental scenarios of velocity …

Omega-6 And Omega-3 Fatty Acid-Derived Oxylipins From The Lipoxygenase Pathway In Maternal And Umbilical Cord Plasma At Delivery And Their Relationship With Infant Growth, Maranda Thompson, Arzu Ulu, Ana G. Yuil-Valdes, Maheswari Mukherjee, Melissa K. Thoene, Matthew Van Ormer, Rebecca Slotkowski, Elizabeth Lyden, Ann Anderson-Berry, Corrine K. Hanson, Tara M. Nordgren, Sathish Kumar Natarajan

Department of Nutrition and Health Sciences: Faculty Publications

Omega-3 and omega-6 fatty acids are important for neonatal development and health. One mechanism by which omega-3 and omega-6 fatty acids exert their effects is through their metabolism into oxylipins and specialized pro-resolving mediators. However, the influence of oxylipins on fetal growth is not well understood. Therefore, the objective of this study was to identify oxylipins present in maternal and umbilical cord plasma and investigate their relationship with infant growth. Liquid chromatography–tandem mass spectrometry was used to quantify oxylipin levels in plasma collected at the time of delivery. Spearman’s correlations highlighted significant correlations between metabolite levels and infant growth. They …

Scavenger Receptors In Host Defense: From Functional Aspects To Mode Of Action, Qamar Taban, Peerzada Tajamul Mumtaz, Khalid Z. Masoodi, Ehtishamul Haq, Syed Mudasir Ahmad

Department of Nutrition and Health Sciences: Faculty Publications

Scavenger receptors belong to a superfamily of proteins that are structurally heterogeneous and encompass the miscellaneous group of transmembrane proteins and soluble secretory extracellular domain. They are functionally diverse as they are involved in various disorders and biological pathways and their major function in innate immunity and homeostasis. Numerous scavenger receptors have been discovered so far and are apportioned in various classes (A-L). Scavenger receptors are documented as pattern recognition receptors and known to act in coordination with other co-receptors such as Toll-like receptors in generating the immune responses against a repertoire of ligands such as microbial pathogens, non-self, intracellular …

Effects Of Increasing Dietary Zinc Sulfate Fed To Gestating Ewes: Ii. Milk Somatic Cell Count, Microbial Populations, And Fatty Acid Composition, C. M. Page, R. M. Knuth, T. W. Murphy, D. C. Rule, B. Bisha, J. B. Taylor, W. C. Stewart

Roman L. Hruska U.S. Meat Animal Research Center: Reports

Objective: The objective of the research was to evaluate the effects of increasing dietary Zn sulfate concentration for primiparous gestating ewes on subsequent milk SCC, intramammary microbial identifications, and fatty acid composition.

Materials and Methods: Commercial white-face (WF; n = 27) and black-face (BF; n = 24) ewes (age ≈18 mo; BW = 87.48 ± 8.37 kg) were sorted into breed-type groups and within groups ranked by BW, and then, they were randomly divided into 3 dietary supplement treatment groups: CON (n = 13; 40 mg/kg Zn; ≈1 × NASEM recommendations), Zn500 (n = 21; 500 mg/kg Zn; …

A Unified Dataset Of Colocated Sewage Pollution, Periphyton, And Benthic Macroinvertebrate Community And Food Web Structure From Lake Baikal (Siberia), Michael F. Meyer, Ted Ozersky, Kara H. Woo, Kirill Shchapov, Aaron W. E. Galloway, Julie B. Schram, Daniel D. Snow, Maxim A. Timofeyev, Dmitry Yu. Karnaukhov, Matthew R. Brousil, Stephanie E. Hampton

School of Natural Resources: Faculty Publications

Sewage released from lakeside development can introduce nutrients and micropollutants that can restructure aquatic ecosystems. Lake Baikal, the world’s most ancient, biodiverse, and voluminous freshwater lake, has been experiencing localized sewage pollution from lakeside settlements. Nearby increasing filamentous algal abundance suggests benthic communities are responding to localized pollution. We surveyed 40-km of Lake Baikal’s southwestern shoreline from 19 to 23 August 2015 for sewage indicators, including pharmaceuticals, personal care products, and microplastics, with colocated periphyton, macroinvertebrate, stable isotope, and fatty acid samplings. The data are structured in a tidy format (a tabular arrangement familiar to limnologists) to encourage reuse. Unique …

Adaptive Capacity Beyond The Household: A Systematic Review Of Empirical Social-Ecological Research, S. Vallury, S. Banerjee, D. Twidwell Jr., D. Uden, C. Allen

School of Natural Resources: Faculty Publications

No abstract provided.

Long Term Agroecosystem Research Experimental Watershed Network, D. C. Goodrich, D. Bosch, R. Bryant, M. H. Cosh, D. Endale, T. Veith, P. Kleinman, E. Langendoen, G. Mccarty, F. Pierson, H. Schomberg, D. Smith, P. Starks, T. Strickland, T. Tsegaye, T. Awada, H. Swain, J. Derner, B. Bestelmeyer, M. Schmer, J. Baker, B. Carlson, D. Huggins, D. Archer, G. Armendariz

School of Natural Resources: Faculty Publications

No abstract provided.

Mind The Gaps For The Best Practices: Enhancing The Management Of Lake Victoria Fisheries Resources, Christopher Mulanda Aura, Amber Roegner, Horace Owiti, Dorothy Birungi, Kathryn J. Fiorella, Jessica R. Corman, Robert Kayanda, Patrick Mbullo, Chrisphine S. Nyamweya, Geofrey Mchau, Miles Daniels, Richard Oginga Abila

School of Natural Resources: Faculty Publications

Fisheries resources face a confluence of socio-ecological challenges, the resolution of which requires interdisciplinary scientific information for sustainable utilization and management. The present study assessed gaps and challenges in Lake Victoria fisheries resources management for better research focus, policy formulation and improved governance of the fishery towards sustainability. Using key informant interviews and a plenary discussion with trans-disciplinary experts regarding Lake Victoria fisheries research, management and policy sectors, the present study identified current management challenges, gaps and priorities. The present study results indicate a constantly increasing fishing effort, poor enforcement of existing regulations and pollution and invasive weeds pose the …

Evaluation Of Aggregate Stability Methods For Soil Health, E. L. Reike, D. K. Bagnall, C.L.S. Morgan, K. D. Flynn, Andrew E. Suyker Et Al.

School of Natural Resources: Faculty Publications

No abstract provided.

Selecting Soil Hydraulic Properties As Indicators Of Soil Health: Measurement Response To Management And Site Characteristics, D. K. Bagnall, C.L.S. Morgan, G. Mac Bean, D. Liptzin, Andrew E. Suyker Et Al.

School of Natural Resources: Faculty Publications

No abstract provided.

Remnant Prairies And High-Diversity Restorations Work Together To Support Wild Bees Season-Long, Katie Lamke, David A. Wedin, Judy Wu-Smart

School of Natural Resources: Faculty Publications

The presence of diverse bee communities in an ecosystem is vital for maintaining healthy plant communities, promoting habitat resilience, and supporting agriculture. In the US, wild bee declines have led to increased monitoring efforts, but there remain critical data gaps in much of the Midwest. Here, we sought to examine how variation in richness and abundance of flowering forbs influences the richness and abundance of wild bees across “remnant” tallgrass prairies, “high diversity” prairie restorations, and “low diversity” prairie restorations or degraded grasslands in eastern Nebraska. High-diversity plots attracted no bees in the early season due to a lack of …

Research And Creative Activity, July 01, 2021-June 30, 2022: Major Sponsored Programs And Faculty Accomplishments In Research And Creative Activity, University Of Nebraska-Lincoln, Office Of Research And Economic Development, University Of Nebraska-Lincoln

Office of Research and Economic Development: Publications

Foreword by Bob Wilhelm, Vice Chancellor for Research and Economic Development:

This booklet highlights successes in research, scholarship and creative activity by University of Nebraska–Lincoln faculty during the fiscal year running July 1, 2021, to June 30, 2022.

It lists investigators, project titles and funding sources on major grants and sponsored awards that were active during the year; fellowships and other recognitions and honors bestowed on our faculty; books, chapters and creative literature published by faculty; performances, exhibitions and other examples of creative activity; patents and licensing agreements; and conference presentations. In recognition of the important role faculty play in …

‘Read For Nutrition’ Programme Improves Preschool Children’S Liking And Consumption Of Target Vegetable, Maha Elrakaiby, Saima Hasnin, Virginia C. Stage, Dipti Dev

Department of Child, Youth, and Family Studies: Faculty Publications

Objective: To determine whether the ‘Read for Nutrition’ programme would increase liking and consumption of broccoli (a target vegetable) in preschool children and test acceptability and practicality of the programme.

Design: Pilot pre-post intervention study, where childcare teachers received training and coaching followed by reading the book ‘Monsters Don’t Eat Broccoli’ multiple times with the children during a three-week intervention.

Setting: Five classrooms of Educare, Lincoln, Nebraska in 2018.

Participants: Sixty-nine (11 to 16 children per classroom) preschool-aged children and sixteen teachers (minimum, three per classroom).

Results: Average total consumption of broccoli increased 35 % (0·14 ounces or 0·05th cup) …

Correlates Of Children’S Dietary Intake In Childcare Settings: A Systematic Review, Saima Hasnin, Jaclyn A. Saltzman, Dipti A. Dev

Department of Child, Youth, and Family Studies: Faculty Publications

Context: Children consume up to two-thirds of their daily dietary requirements in full-time childcare, making the setting a critical vector for preventing childhood obesity. Objective: To summarize the ecological correlates of children’s dietary intake in childcare settings that were identified and categorized using the Six-Cs developmental ecological model of contributors to overweight and obesity in childhood. Data Sources: A literature search was conducted in 4 electronic databases. Study Selection: English-language, peer-reviewed publications that investigated at least 1 correlate of children’s (ages 2–6 years) dietary intake in childcare settings and measured children’s actual consumption of foods and beverages from food groups …

Genome-Wide Association Study Of Disease Resilience Traits From A Natural Polymicrobial Disease Challenge Model In Pigs Identifies The Importance Of The Major Histocompatibility Complex Region, Jian Cheng, Rohan Fernando, Hao Cheng, Stephen D. Kachman, Kyusang Lim, John C.S. Harding, Michael K. Dyck, Frederic Fortin, Graham S. Plastow, Piggen Canada Research Consortium, Jack C.M. Dekkers

Department of Statistics: Faculty Publications

Infectious diseases cause tremendous financial losses in the pork industry, emphasizing the importance of disease resilience, which is the ability of an animal to maintain performance under disease. Previously, a natural polymicrobial disease challenge model was established, in which pigs were challenged in the late nursery phase by multiple pathogens to maximize expression of genetic differences in disease resilience. Genetic analysis found that performance traits in this model, including growth rate, feed and water intake, and carcass traits, as well as clinical disease phenotypes, were heritable and could be selected for to increase disease resilience of pigs. The objectives of …