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Targeting Of Tsc13p To Nucleus–Vacuole Junctions: A Role For Very-Long-Chain Fatty Acids In The Biogenesis Of Microautophagic Vesicles, Erik Kvam, Kenneth Gable, Teresa M. Dunn, David S. Goldfarb

Uniformed Services University of the Health Sciences

TSC13 is required for the biosynthesis of very-long-chain fatty acids (VLCFAs) in yeast. Tsc13p is a polytopic endoplasmic reticulum (ER) membrane protein that accumulates at nucleus–vacuole (NV) junctions, which are formed through Velcro-like interactions between Nvj1p in the perinuclear ER and Vac8p on the vacuole membrane. NV junctions mediate piecemeal microautophagy of the nucleus (PMN), during which bleb-like portions of the nucleus are extruded into invaginations of the vacuole membrane and degraded in the vacuole lumen. We report that Tsc13p is sequestered into NV junctions from the peripheral ER through Vac8p-independent interactions with Nvj1p. During nutrient limitation, Tsc13p is incorporated …

University Of Nebraska- Lincoln: Fact Book 2004-2005

University of Nebraska-Lincoln Administration: Reports, Papers, Publications, and Presentations

Fact Book 2004-2005 Table of Contents

General Information

Role and Mission Statement....................................................................4

Institutional & Professional Accreditations......................................................7

UNL Organizational Chart..................................................................9

Student Credit Hours

Student Credit Hours UNL Student Credit Hours Total Fall, by College 2000 to 2004................................................................10

UNL Student Credit Hours Total Spring, By College 2000 to 2004....................................................11

UNL Student Credit Hours Total Fall & Spring Semester, by College 1999—2003..........................12

Summer Sessions 2004 Student Credit Hours..........................................13

Retention, Degrees and Majors

Retention, Degrees and Majors UNL Student Retention & Graduation Rate Analysis..................................................................................14

Total Degrees conferred by UNL Fiscal Year 1994-95 to 2003-2004............................................................15

Degrees Conferred July 1, 2003 through June 30, 2004...............................................................................16

Types …

Heating Systems For Wean-To-Finish Facilities, Mike Brumm, Sheryl Colgan, Richard R. Stowell

Nebraska Swine Reports

Research was conducted to assess the effects of the type of zone heater and floor mat used in a wean-to-finish facility on pig performance and operating cost. Gas-fired brooder heaters were compared to 250W heat lamps and farm-cut wood sheathing was compared to commercial (unheated) rubber floor mats for a 21-day post-weaning period. There was no effect of heating system or mat type on pig performance, either during the 21- day period immediately post-weaning or to slaughter. Black globe temperatures near the pig zone were slightly higher for the propane-fired heaters compared to the heat lamps, most likely due to …

Incidence And Inheritance Of Splayleg In Nebraska Litter Size Selection Lines, Justin W. Holl, Rodger K. Johnson

Nebraska Swine Reports

Incidence of abnormalities at birth is low in most populations, but accounts for a significant proportion of preweaning deaths. Splayleg pigs (SL) is the most common defect in newborn pigs and a high percentage of SL pigs die before weaning. In research at other institutions, SL incidence was associated with the Landrace breed and with large litters; however, a genetic association with litter size was not demonstrated. The University of Nebraska selection lines originated from a Landrace-Large White composite population and have been selected for 22 generations for increased litter size. These lines provided an excellent resource for the objectives …

Research Proposal Summary: Effects Of Nutrition During Gilt Development On Sow Lifetime Productivity Of Two Prolific Maternal Lines, Rodger K. Johnson, Phillip S. Miller

Nebraska Swine Reports

A four-parity study is proposed to examine the effects of nutritional manipulation during gilt development on subsequent sow performance. Gilts will be selected from two genetic lines that are highly prolific, but differ in rate and composition of growth. During the development period (45 to 250 lb), gilts will be provided either; i) ad libitum access to feed, or ii) ad libitum access to feed until 120 days of age (180 lb) and 75% of ad libitum feed intake thereafter (until first post-pubertal estrus). Sow and litter performance criteria will be examined. In addition, the economics of sow productivity and …

Prrsv Negative Herds: A Survival Analysis, Locke Karriker, Ruby Destajo

Nebraska Swine Reports

Despite a significant body of research, interventions for PRRSV infection remain elusive. Traditional approaches to managing the risk of diseases have not been successful in many cases of PRRSV infection. While elimination of the virus from farms is possible, it is not without cost and re-infection is common. This survey sought to quantify the expected duration of PRRSV negative status on farms that were repopulated with PRRSV negative animals or had undergone a PRRSV elimination program. Results of 96 cases reveals a range of <1 to 312+ weeks duration of negative status. A survival analysis of 84 farms revealed a probability of surviving with negative status for two full years of 58.3% with a standard error of 11.5%. The probability of survival through 4 years was 42% with a standard error of 16%. A greater percentage of farms that were reinfected shared resources such as equipment, personnel, and/or vehicles with known positive farms. Positive farms also had a relatively shorter average distance to known positive farms than those remaining negative. The results of this study indicate that PRRSV-negative farms are not very likely to remain negative for a long duration given current technologies. Longer survival of negative status appears to be associated with greater distance from known positive farms and stricter biosecurity. Sharing of equipment and other resources as well as a closer distance to other farms should be considered risk factors that can lessen the probability of farms maintaining negative status.

2005 Nebraska Swine Report

Nebraska Swine Reports

2005 Nebraska Swine Report

Measurement Equivalence Of The Center For Epidemiological Studies Depression Scale For Latino And Anglo Adolescents: A National Study, Lisa J. Crockett, Brandy A. Randall, Yuh-Ling Shen, S T. Russell, Anne K. Driscoll

Department of Psychology: Faculty Publications

The cross-ethnic measurement equivalence of the Center for Epidemiologic Studies Depression Scale (CES-D; L. S. Radloff, 1977) was examined using a subsample of adolescents (N = 10,691) from the National Longitudinal Study of Adolescent Health, Configural and metric invariance, as well as functional and scalar equivalence, were examined for Anglo American, Mexican American, Cuban American, and Puerto Rican American youths age 12-18 years. Confirmatory factor analysis (CFA) in each group provided evidence of configural invariance for European and Mexican American adolescents but not for Cuban and Puerto Rican youths. A 2-group CFA for Anglo and Mexican Americans demonstrated partial metric …

Comparison Of Two Development Systems For March-Born Replacement Beef Heifers, Kelly W. Creighton, Jacqueline A. Musgrave, Terry J. Klopfenstein, Richard T. Clark, Don C. Adams

Nebraska Beef Cattle Reports

A three-year study (2001-2003) was conducted to determine the effect of development system on reproductive performance of first-calf heifers. March-born heifers (n=261) were developed to reach either 55% of mature body weight (MBW) before a 45-day breeding season or 50% of MBW before a 60-day breeding season. Extending the breeding season 15 days for heifers developed to 50% of MBW prior to the first breeding season resulted in equal pregnancy, calving and weaning rates to heifers developed to 55% of MBW. Furthermore, the reduction in development costs in the 50% of MBW system more than offset the reduced income from …

Direct-Fed Microbial Products For Escherichia Coli O157:H7 In Market Ready Feedlot Cattle, Robert E. Peterson, David R. Smith, Jeffrey D. Folmer, Galen E. Erickson, Susan Hinkley, Rodney A. Moxley

Nebraska Beef Cattle Reports

A clinical trial was conducted during the summers of 2002 and 2003 to evaluate the effect of a direct-fed microbial product (DFM) on the prevalence of E. coli O157:H7 in feces of feedlot steers. The DFM consisted of Lactobacillus acidophilus (NPC 747) fed at the rate of 1x109 colony forming units (CFUs) per head per day. Treatments included supplemental DFM or no supplemental DFM. Feedlot steers supplemented with DFM were 35% less likely to shed E. coli O157:H7 in the feces compared with steers that were not supplemented with the DFM. Finishing performance was not affected by adding a DFM …

Evaluation Of Cocoa- And Coffee-Derived Methylxanthines As Toxicants For The Control Of Pest Coyotes, John J. Johnston

United States Department of Agriculture Wildlife Services: Staff Publications

Methylxanthines were quantified in coffee, tea, and chocolate products. Tarajuilie tea from India, cocoa powder, and cocoa nibs contained the highest levels of methylxanthines. Theobromine, caffeine, and theophylline combined in the ratios observed in tea and chocolate were ingested by coyotes. Although both mixtures induced acute toxicity, the symptoms accompanying the chocolate methylxanthine mimic were preferable. Manipulation of the ratios of methylxanthines in the chocolate mimic led to the identification of a 5:1 theobromine/caffeine mixture as a promising coyote toxicant. This mixture was then administered to coyotes using the coyote lure operative device (CLOD). Mortality occurred in every coyote that …

Quality Of Transgenic Laboratory Strains Of Cochliomyia Hominivorax (Diptera: Calliphoridae), Margaret L. Allen, Philip J. Scholl

Department of Entomology: Faculty Publications

Genetically modified, mass reared insects present novel possibilities for the future of insect control. One concern about manipulation of insects is a possible loss of strain quality due to the introduction of a foreign gene of any sort into the insect genome. Eight transgenic strains of screwworm, Cochliomyia hominivorax (Coquerel) (Diptera: Calliphoridae), were compared with the wild-type parental laboratory strain in laboratory culture. Measurements of average fertility, fecundity, larval productivity, and longevity were analyzed. Two transgenic strains had significantly lower larval productivity than controls, one of which was explained by a homozygous lethal insertion of the transgene. Another strain produced …

Grazing Evaluation Of Big Bluestems Bred For Improved Forage Yield And Digestibility, R. B. Mitchell, Kenneth P. Vogel, T. J. Klopfenstein, B. E. Anderson, R. A. Masters

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Two big bluestem (Andropogon gerardii Vitman) strains (Pawnee C3 and Kaw C3) developed by three breeding cycles for increased forage yield and in vitro dry matter digestibility (IVDMD) were compared with the base populations of 'Pawnee' and 'Kaw' in a 3-yr grazing trial. Pastures were seeded in May 1998 near Mead, NE. Experimental units were three 0.4-ha pastures of each strain or cultivar in a randomized complete block design. Pastures were burned and fertilized with ammonium nitrate at 112 kg N ha^-1 in the spring of 2000, 2001, and 2002 before grazing. Each pasture was continuously stocked with …

Evaluation Of Oviposition Substrates And Organic Infusions On Collection Of Culex In Florida, Sandra Allan, Ulrich R. Bernier, David Kline

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Gravid mosquito traps are commonly used for both arbovirus surveillance and population surveillance of mosquitoes of the genus Culex. Oviposition substrates, used as baits in these traps, were tested against Culex under laboratory and field conditions. In the laboratory all substrates tested as 1% and 10% dilutions in 2-choice bioassays against female Cx. quinquefascians were significantly more effective than well water controls in eliciting oviposition. Strongest responses were to dilutions of dairy effluent, followed by larval water and infusions of alfalfa hay, alfalfa pellets, Bermuda hay, oak leaves, and Typha leaves, with lowest responses to cow manure infusion. …

Influence Of Dietary Crude Protein Concentration And Source On Potential Ammonia Emissions From Beef Cattle Manure, N. A. Cole, R. N. Clark, R. W. Todd, C. R. Richardson, A. Gueye, L. W. Greene, K. Mcbride

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Emissions of ammonia, as well as other gases and particulates, to the atmosphere are a growing concern of livestock producers, the general public, and regulators. The concentration and ruminal degradability of CP in beef cattle diets may affect urinary and fecal excretion of N and thus may affect ammonia emissions from beef cattle feed yards. To determine the effects of dietary CP concentration and degradability on potential ammonia emissions, 54 steers were randomly assigned to nine dietary treatments in a 3 × 3 factorial arrangement of treatments. Treatments consisted of three dietary CP concentrations (11.5, 13, and 14.5%) and three …

Involvement Of Acetosyringone In Plant–Pathogen Recognition, C. Jacyn Baker, Norton M. Mock, Bruce D. Whitaker, Daniel P. Roberts, Clifford P. Rice, Kenneth L. Deahl, Andrey A. Aver'yanov

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

In this study, acetosyringone was identified as one of the major extracellular phenolics in tobacco suspension cells and was shown to have bioactive properties that influence early events in plant–bacterial pathogenesis. In our model system, tobacco cell suspensions treated with bacterial isolate Pseudomonas syringae WT (HR+) undergo a resistant interaction characterized by a burst in oxygen uptake several hours after inoculation. When the extracellular concentration of acetosyringone in tobacco cell suspensions was supplemented with exogenous acetosyringone, the burst in oxygen uptake occurred as much as 1.5 h earlier. The exogenous acetosyringone had no effect on tobacco suspensions undergoing susceptible interactions …

Induction Of Redox Sensitive Extracellular Phenolics During Plant–Bacterial Interactions, C. Jacyn Baker, Bruce D. Whitaker, Daniel P. Roberts, Norton M. Mock, Clifford P. Rice, Kenneth L. Deahl, Andrey A. Aver'yanov

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

This study focuses on the transient and complex nature of phenolics that accumulate in the extracellular environment of plant suspension cells during the first few hours of the interaction between these plant cells and bacterial pathogens. Using suspension cells of Nicotiana tabacum we identified four acetophenones and four hydroxycinnamic acid amides that accumulate in this extracellular environment. Treatment of the suspension cells with isolates of the plant pathogen Pseudomonas syringae or heat-killed bacteria increased elicitation of extracellular phenolics and changed the composition of the compounds that accumulated. These phenolics were sensitive to oxidative stress; when suspension cells were treated with …

Lamb Production Of Dorper, Katahdin, And St. Croix Bred In Summer, Winter, Or Spring In The Southeastern United States, J. M. Burke

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Ewe production traits and ability to breed out of season were compared for the Dorper (DO), Katahdin (KA), and St. Croix (SC) breeds between 2000 and 2005. Sheep were managed on grass pasture and were supplemented with corn/soybean meal and free-choice, trace-mineral mix. Ewes were exposed to rams of their respective breeds in late summer (August/September), winter (December), or spring (April/ May) for 30-day breeding periods. Lambs were weighed at birth and 60 days of age. Pregnancy and lambing rates and litter birth weight were greater for all breeds bred in winter and lowest in spring. Pregnancy losses were greater …

Growth Performance Of Barbados Blackbelly, Katahdin And St. Croix Hair Sheep Lambs Fed Pasture- Or Hay-Based Diets, S. Wildeus, K. E. Turner, J. R. Collins

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Two experiments evaluated growth of mixed-sex Barbados Blackbelly, Katahdin, and St. Croix hair sheep lambs raised on pasture or hay-based diets with moderate levels of energy supplementation. In Experiment 1, 36 ewe and wether lambs were allocated to a pasture or pen feeding group in May. Pasture animals rotationally grazed tall fescue pasture, while pen animals were offered chopped alfalfa hay, and both groups were supplemented with corn/soybean meal at 0.75% of body weight. In Experiment 2, 72 lambs were allocated to pen and pasture in April, and provided either a low or high crude protein concentration corn/soybean meal supplement …

Composition Of Irrigation Water Salinity Affects Growth Characteristics And Uptake Of Selenium And Salt Ions By Soybean, D. Wang, C. M. Grieve, D. L. Suarez

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

A greenhouse study was conducted to determine the effects of irrigation waters differing in salt composition on growth characteristics, salt ion and selenium (Se) accumulation, and distribution in plant components of the soybean (Glycine max L. Merr.) cultivar “Manokin.” Plants were grown in sand cultures and irrigated with isoosmotic solutions containing (1) Cl⁻ as the dominant anion, or (2) a mixture of salts containing equal molar amounts of SO_^2-4  and Cl⁻. Six treatments of each salinity type were imposed. Electrical conductivities of the irrigation waters ranged from 2.1 to 13.0 dSm⁻¹. Selenium …

The Degree Of Chlmpanzee Theory Of Mind And The Evolution Of Modularity, Benjamin Grant Purzycki

Transactions of the Nebraska Academy of Sciences and Affiliated Societies

Whether or not chimpanzees have the ability to mentally represent others' mental states or theory of mind (ToM) has yet to be definitively established. This results from three problems. First, modular theory of mind accounts lead researchers to adopt an either/or approach to psychological faculties which obfuscates both within- and across-species variability. Second, present research continues to rely on the continued trend to polarize nature and nurture. Third, the bulk of the work compares humans with chimpanzees rather than looking at the entire range of primate species. I propose "degree approach" by way of the Integrated Causal Model which particularizes …

Ctla-4 Signaling Regulates The Intensity Of Hypersensitivity Responses To Food Antigens, But Is Not Decisive In The Induction Of Sensitization, Femke Van Wijk, Sanne Hoeks, Stefan Nierkens, Stef J. Koppelman, Peter Van Kooten, Louis Boon, Leon M.J. Knippels, Raymond Pieters

Department of Food Science and Technology: Faculty Publications

Although food allergy has emerged as a major health problem, the mechanisms that are decisive in the development of sensitization to dietary Ag remain largely unknown. CTLA-4 signaling negatively regulates immune activation, and may play a crucial role in preventing induction and/or progression of sensitization to food Ag. To elucidate the role of CTLA-4 signaling in responses to food allergens, a murine model of peanut allergy was used. During oral exposure to peanut protein extract (PPE) together with the mucosal adjuvant cholera toxin (CT), which induces peanut allergy, CTLA-4 ligation was prevented using a CTLA-4 mAb. Additionally, the effect of …

Decline And Recovery Of A High Arctic Wolf-Prey System, L. David Mech

United States Geological Survey, Northern Prairie Wildlife Research Center: Publications

A long-existing system of wolves (Canis lupus), muskoxen (Ovibos moschatus), and arctic hares (Lepus arcticus) in a 2600 km² area of Canada’s High Arctic (80° N latitude) began collapsing in 1997 because of unusual adverse summer weather but recovered to a level at which all three species were reproducing by 2004. Recovery of wolf presence and reproduction appeared to be more dependent on muskox increase than on hare increase.

Un vieux système biologique composé de loups (Canis lupus), de boeufs musqués (Ovibos moschatus) et de lièvres arctiques (Lepus …

Bears Remain Top Summer Predators, Shannon M. Barber, L. David Mech, P. J. White

United States Geological Survey, Northern Prairie Wildlife Research Center: Publications

In the ten years since wolves (Canis lupus) were restored to Yellowstone National Park (YNP), elk (Cervus elaphus) numbers have substantially decreased. The northern range elk herd is the largest elk herd in Yellowstone, and constitutes the majority of the park’s elk population. During 1994–2005, early winter counts of northern Yellowstone elk decreased from 19,045 to 9,545. Also, during winters 2000–2004, calf:cow ratios declined from 29:100 to 12:100, and were among the lowest recorded during the past several decades. Though many factors (e.g., predation, hunting, and drought) likely contributed to this decreasing abundance and low recruitment, …

Replication And Encapsidation Of Papillomaviruses In Saccharomyces Cerevisiae, Peter C. Angeletti

Nebraska Center for Virology: Faculty Publications

Improvements in methodologies to recapitulate and study particular biological functions of the

papillomavirus life cycle have led to great advances in our knowledge of these viruses. Described in

this chapter are techniques that allow low-copy and high-copy replication of full-length human

papillomavirus (HPV) genomes, as well as assembly of virus-like particles, in Saccharomyces

cerevisiae (yeast). This system has several distinct advantages that make it an attractive complement

to the well-established raft-culturing system. First, yeast are inexpensive, rapid, and simple to culture

in the lab. Second, they provide an ever-widening array of genetic tools to analyze HPV functions

—most recently notable, …

Biological Opinion On The Operation Of The Missouri River Main Stem System, Missouri River Bank Stabilization And Navigation Project, And Operation Of The Kansas River Reservoir System

United States Army Corps of Engineers, Omaha District

The U.S. Army Corps of Engineers (Corps) prepares an annual report for interested parties in accordance with reporting requirements of the "Biological Opinion (BiOp) on the Operation of the Missouri River Main Stem System, Operation and Maintenance of the Missouri River Bank Stabilization and Navigation Project (BSNP), and Operation of the Kansas River Reservoir System" prepared by the U.S. Fish and Wildlife Service (Service) dated November 30,2000 and the Amendment, thereto, dated December 16,2003.

This annual report documents the Corps threatened and endangered species conservation activities to comply with the provisions of the 2000 BiOp and the 2003 Amendment to …

Husbandry Of Wild-Caught Greater Sage-Grouse, Paul Oesterle, Robert Mclean, Michael Dunbar, Larry Clark

United States Department of Agriculture Wildlife Services: Staff Publications

This study reports the first successful husbandry and breeding in captivity of wild-caught greater sage-grouse (Ccntroccrcus urophasianus). In October 2003, 2 1 hatch-year greater sage-grouse were trapped in northwestern Nevada and transported to Fort Collins, Colorado. We held grouse in pens at the United States Department of Agriculture's National Wildlife Research Center for 8 months. We offered a varied diet, including native food items such as sagebrush (Arternisia tridentata and A. tripartita) and yarrow (Achilles millefolium). We housed grouse in a large flight pen and allowed to them freerange as one flock. Mortdlity rate was 16.7%. Several of the grouse …

Marketing Locally Produced Foods: Consumer And Farmer Opinions In Washington County, Nebraska, Mindi L. Schneider, Charles A. Francis

Department of Agronomy and Horticulture: Faculty Publications

Local food system potentials were studied in Washington County, Nebraska, United States. As a departure from most studies of locally based systems, farmers were surveyed in addition to consumers for potential participation. Data about the current food system and opinions and preferences for local production, marketing, and purchasing of food were collected using self-administered mail questionnaires. The response rate was 35% for the farmer survey and 37% for the consumer survey. Results indicated that, on the farming side of the food system, conventional corn and soybean production and marketing predominated in Washington County, and farmer interest in producing for local …

Species Diversity And Functional Composition Of Pastures That Vary In Landscape Position And Grazing Management, John A. Guretzky, Kenneth J. Moore, E. Charles Brummer, C. Lee Burras

Department of Agronomy and Horticulture: Faculty Publications

The productivity of grasslands depends in part on their diversity of species and functional composition. Our objective was to examine the effects of three landscape positions (summit, backslope, and toe-slope) and three stocking systems (continuous, rotational, and non-grazed) on species diversity and percentage of cover of grass, legume, and weed species functional types in southeastern Iowa pastures.Data were collected in 0.2-m² plots randomly distributed throughout each of four replicate pastures in spring and summer 2000 and 2001. Backslope landscape positions within pastures managed with either continuous or rotational stocking contained the greatest overall diversity of species. Across years, overall …

Evaluating Livestock System Environmental Performance With Whole-Farm Nutrient Balance, Richard K. Koelsch

Department of Agricultural and Biological Systems Engineering: Faculty Publications

As a part of the USEPA’s concentrated animal feeding operation (CAFO) final rule, all CAFOs are required to develop and implement a nutrient management plan (NMP). The USEPA’s emphasis on better management of nutrients appropriately targets a critical environmental issue associated with animal production. The concentration of animals in livestock feeding operations, often separate from feed grain production, requires importing of substantial quantities of feed nutrients. Due to the inefficiencies of nutrient utilization in livestock production, quantities of nitrogen (N) and phosphorus (P) in manure greater than can be utilized in local crop production often result. With the focus of …