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Agricultural Research Magazine, Vol. 62, No. 4, April 2014, United States Department Of Agriculture

Agricultural Research Magazine

Forum-ARS Plays an Integral Role in Combating a Virulent Threat Targeting Pheromones in Fire Ants Male Chromosome Hinders Female Cattle Reproduction Nitrite Significant Role in Nitrous Oxide Emissions from Soil New-and More Economical-Pyrolysis Techniques for Bio-oil Production New Ornamental Tung Tree a Story of Loss and Restorations Fighting a Pernicious Weed with Fire Looking to Wheat's Wild Ancestors to Solve a Modern Problem Locations Featured in This Magazine Issue New Food Ingredient from Rice Bran Oil May Help Make Foods’ Fat Profile More Healthful

Agricultural Research Magazine, Vol. 62, No. 5, May/June 2014, United States Department Of Agriculture

Agricultural Research Magazine

Forum—Valuing U.S. Agriculture Identifying Yellow Perch Gender to Improve Production "Supershedders" Role in Spreading E. coli Scrutinized Transparent Coating for Glass, Plastics—and More—May Have Bright Future Foraging Worms Guarantee Slug Bait Failure Dissecting a Potato Pathogen's Hiding Place in Its Insect Vector Advance May Speed Development of Seed Rot-Resistant Soybeans Taming Extreme Environments by Exploring Algae in Space Pigs Useful in Immune and Obesity Research Controlling Odors and Greenhouse Gas Emissions from Stored Swine Manure New Class of Animal Deworming Agent Locations Featured in This Magazine Issue

Agricultural Research Magazine, Vol. 62, No. 6, July 2014, United States Department Of Agriculture

Agricultural Research Magazine

Forum—Enhancing Food Safety by Supporting Regulatory Agencies and Industry A Lab That Keeps Us All Out of a Pickle Genetic Mapping of Rust-resistance Genes in Sunflower Orange-Fleshed Honeydew Melon: Ripe for Beta-Carotene Analysis Making a Barley Fish Feed Product Details Matter in Modeling Carbon Sequestration and Cropping Systems Reducing Fertilizer Needs by Accounting for Soil Microbes Data Analyses Show How Weather Patterns Can Affect Cattle Production Whacking Weeds Organically Treatment Has Potential to Reduce Allergenicity of Peanuts Locations Featured in This Magazine Issue

Agricultural Research Magazine, Vol. 62, No, 9, October 2014, United States Department Of Agriculture

Agricultural Research Magazine

Forum-Keeping Pace with Agriculture's Challenges New Imaging Techniques Leads to Better Understanding of Freezing in Plants Better Information for Improved Beef Manure Management Improving Trout Resistance to a Deadly Disease Pest's Taster for Fine Wine May Prove Its Undoing Mandarin Oranges: Protecting the Flavor of This Popular Fruit Experimental Website Helps Teens Eat More Veggies Latest Update of USDA's Nutrient Database Released The Greatest Show on Turf Assessing Cotton Fiber Quality From a Tiny Sample A Steady Pace of Cellulosic Ethanol Research in Peoria Using Location Information to Improve Literature Searches Locations Featured in This Magazine Issue

Agricultural Research Magazine, Vol. 62, No. 10, November/December 2014, United States Department Of Agriculture

Agricultural Research Magazine

Forum-The Never Ending Insect Control Contest Fire Ants Are No Match for Poison Frogs Controlling Native Stink Bugs in the Southeast Closing in on Butanol for Biofuel Assessing the Ricks of HoBi-like Virus in Cattle Making Peach Trees Fit Better on the Field Finding Rice Traits That Tackle Climate-change Challenges New Test Count Total Phenolics in Fruits and Vegetables Delicious, Nutritious, and a Colorful Dish for the Holidays! Markers Highlight the Way to Disease Resistance in Peas Your Brain and Comfort Foods: Neuroimages Capture Effect Locations Featured in This Magazine Issue

Fitness In Animals Correlates With Proximity To Discontinuities In Body Mass Distributions, David G. Angeler, Craig R. Allen, Anna Vila-Gispert, David Almeida

Nebraska Cooperative Fish and Wildlife Research Unit: Staff Publications

Discontinuous structure in landscapes may cause discontinuous, aggregated species body-mass patterns, reflecting the scales of structure available to animal communities within a landscape. Empirical analyses have shown that the location of species within body mass aggregations, which reflect this scalespecific organization, is non-random with regard to several ecological phenomena, including species extinctions. The propensity of declining species to have body masses proximate to discontinuities suggests that transition zones between scaling regimes ultimately decreases the ecological fitness for some species. We test this proposition using vulnerable and unthreatened fish species in Mediterranean streams with differing levels of human impact. We show …

Severe Morbidity And Mortality Risk From Malaria In The United States, 1985–2011, Jimee Hwang, Karen A. Cullen, S. Patrick Kachur, Paul M. Arguin, J. Kevin Baird

United States Public Health Resources

Background. Recent reports of Plasmodium vivax associated with severe syndromes and mortality from malaria endemic areas questions the “benign” course of non-falciparum malarias.

Methods. We retrospectively analyzed data from patients reported to the US Centers for Disease Control and Prevention with a diagnosis of malaria parasite single-species infection between 1985 and 2011. Patients classified as having severe illness were further classified according to outcome (survival versus death) and clinical syndrome.

Results. Among all cases, .9% of Plasmodium falciparum cases resulted in death and 9.3% were classified as severe, whereas .09% of P. vivax cases resulted in death and 1.3% were …

State-Specific Estimates Of Complete Smoke-Free Home Rules Among Postpartum Women, 2010, Van T. Tong, Yalonda Hutchings, Sherry L. Farr, Denise D'Angelo, Stephen Babb

United States Public Health Resources

Background: Secondhand smoke exposure increases an infant's risk of morbidity and mortality. We provide state-specific estimates for and characterize postpartum women with complete smoke-free home rules.

Methods: Data were analyzed from 26 states and New York City (n= 37,698) from the 2010 Pregnancy Risk Assessment Monitoring System, a population-based survey of women who recently delivered live-born infants. We calculated state-specific estimates of complete rules and assessed associations between complete rules and selected characteristics.

Results: Overall, 93.6% (95% CI: 93.1–94.1) of women with recent live births had complete smoke-free home rules (86.8% [West Virginia] to 98.6% [Utah]). Demographic groups with the …

Caregiver Behavior Change For Child Survival And Development In Low- And Middle-Income Countries: An Examination Of The Evidence, John P. Elder, Willo Pequegnat, Saifuddin Ahmed, Gretchen Bachman, Merry Bullock, Waldemar A. Carlo, Venkatrama Chandra- Mouli, Nathan A. Fox, Sara Harkness, Gillian Huebner, Joan Lombardi, Velma Mcbride Murry, Allisyn Moran, Maureen Norton, Jennifer Mulik, Will Parks, Helen Raikes, Joseph Smyser, Caroline Sugg, Michael Sweat

United States Public Health Resources

In June of 2012, representatives from more than 80 countries promulgated a Child Survival Call to Action, which called for reducing child mortality to 20 or fewer child deaths per 1,000 live births in every country by 2035. To address the problem of ending preventable child deaths, the U.S. Agency for International Development and the United Nations Children’s Fund convened, on June 3–4, 2013, an Evidence Summit on Enhancing Child Survival and Development in Lower- and Middle-Income Countries by Achieving Population-Level Behavior Change. Six evidence review teams were established on different topics related to child survival and healthy development to …

Bottle-Feeding Practices During Early Infancy And Eating Behaviors At 6 Years Of Age, Ruowei Li, Kelley S. Scanlon, Ashleigh May, Chelsea Rose, Leann L. Birch

United States Public Health Resources

BACKGROUND: Evidence suggests an association of breastfeeding with a maternal feeding style (MFS) that is less controlling than formula feeding, which, in turn, may improve a child’s self-regulation of eating. This study examines associations of bottle-feeding practices during infancy with MFS and children’s eating behavior (CEB) at 6 years old.

METHODS: We linked data from the Infant Feeding Practices Study II to the Year 6 Follow-Up, which include 8 MFS and CEB measures adapted from previous validated instruments. Bottle-feeding practices during the first 6 months estimated by using the Infant Feeding Practices Study II were bottle-feeding intensity (BFI), mother’s …

Rapid Detection Of Shigella And Enteroinvasive Escherichia Coli In Produce Enrichments By A Conventional Multiplex Pcr Assay, Rachel Binet, Deanne M. Deer, Samantha J. Uhlfelder

United States Public Health Resources

Faster detection of contaminated foods can prevent adulterated foods from being consumed and minimize the risk of an outbreak of foodborne illness. A sensitive molecular detection method is especially important for Shigella because ingestion of as few as 10 of these bacterial pathogens can cause disease. The objectives of this study were to compare the ability of four DNA extraction methods to detect Shigella in six types of produce, post-enrichment, and to evaluate a new and rapid conventional multiplex assay that targets the Shigella ipaH, virB and mxiC virulence genes. This assay can detect less than two Shigella cells …

Aflatoxin M1 And Ochratoxin A In Human Milk In Ribeirão Preto-Sp, Brazil, Maria Helena Iha, Cynara Baltazar Barbosa, Anália Ribeiro Heck, Mary W. Trucksess

United States Public Health Resources

The objective of this study was to determine the extent of aflatoxin M₁ (AFM₁) and ochratoxin A (OTA) contamination in human breast milk in the city of Ribeirão Preto, São Paulo State, Brazil. During 2012, 100 samples of human milk were collected at the local Human Milk Bank. The method comprised, immunoaffinity column purification and isolation, liquid chromatography separation and fluorescence detection. The average percentage recoveries of AFM₁ and OTA spiked at 20 and 50 ng/L in control human milk were 78.1 ± 11.7% and 73.7 ± 9.6%, respectively. The average relative standard deviations of AFM …

Isolation And Molecular Characterization Of Salmonella Enterica Serovar Enteritidis From Poultry House And Clinical Samples During 2010, Ezat H. Mezal, Ashley Sabol, Mariam A. Khan, Nawab Ali, Rossina Stefanova, Ashraf A. Khan

United States Public Health Resources

A total of 60 Salmonella enterica serovar (ser.) Enteritidis isolates, 28 from poultry houses and 32 from clinical samples, were isolated during 2010. These isolates were subjected to testing and analyzed for antibiotic resistance, virulence genes, plasmids and plasmid replicon types. To assess genetic diversity, pulsed-field gel electrophoresis (PFGE) fingerprinting, using the XbaI restriction enzyme, Multiple-Locus Variable-Number Tandem Repeat Analysis (MLVA) and plasmid profiles were performed. All isolates from poultry, and 10 out of 32 clinical isolates were sensitive to ampicillin, chloramphenicol, gentamicin, kanamycin, nalidixic acid, sulfisoxazole, streptomycin, and tetracycline. Twenty-one of thirty-two clinical isolates were resistant to ampicillin and …

Detection Of Five Shiga Toxin-Producing Escherichia Coli Genes With Multiplex Pcr, Insook Son, Rachel Binet, Anna Maounounen-Laasri, Andrew Lin, Thomas S. Hammack, Julie A. Kase

United States Public Health Resources

Escherichia coli serogroup O157 is the pathogen most commonly associated with foodborne disease outbreaks, but epidemiological studies suggest that non-O157 Shiga toxin-producing E. coli (STEC) is a major player as well. The ten most clinically relevant STECs belong to serogroups O26, O103, O111, O145, O157, O91, O113, O128, O45, and O121; but emerging strains, such as O104:H4 that was identified with the 2011 German outbreak, could become more prevalent in the future. A 75-min conventional multiplex PCR assay, IS-5P, targeting the four virulence factors stx1, stx2, eae, and ehxA plus the O157:H7- specific +93 uidA single nucleotide …

Increasing Dietary Neutral Detergent Fiber Concentration Decreases Ruminal Hydrogen Sulfide Concentrations In Steers Fed High-Sulfur Diets Based On Ethanol Coproducts, S. J. Morine, M. E. Drewnoski, S. L. Hansen

Department of Animal Science: Faculty Publications

Cattle feedlot diets commonly contain ethanol coproducts that are high in S. This dietary S is reduced in the rumen by sulfate reducing bacteria, resulting in an accumulation of hydrogen sulfide (H2S), increasing the risk for S toxicity. A negative correlation between H₂S and ruminal pH has been observed previously. The objective of this study was to determine the effect of varying dietary NDF from chopped bromegrass hay (66% NDF) on performance, ruminal pH, and ruminal H2S gas concentration of steers fed a high-S finishing diet. One hundred fifty crossbred steers (359 ± 51 kg BW) were blocked …

Pharmacological Targeting Of The Mammalian Clock Regulates Sleep Architecture And Emotional Behaviour, Subhashis Banerjee, Yongjun Wang, Laura A. Solt, Kristine Griffett, Melissa Kazantzis, Ariadna Amador, Bahaa M. El-Gendy, Salvador Huitron-Resendiz, Amanda J. Roberts, Youseung Shin, Theodore M. Kamenecka, Thomas P. Burris

Department of Nutrition and Health Sciences: Faculty Publications

Synthetic drug-like molecules that directly modulate the activity of key clock proteins offer the potential to directly modulate the endogenous circadian rhythm and treat diseases associated with clock dysfunction. Here we demonstrate that synthetic ligands targeting a key component of the mammalian clock, the nuclear receptors REV-ERBα and β, regulate sleep architecture and emotional behaviour in mice. REV-ERB agonists induce wakefulness and reduce REM and slow-wave sleep. Interestingly, REV-ERB agonists also reduce anxiety-like behaviour. These data are consistent with increased anxiety-like behaviour of REV-ERBβ-null mice, in which REV-ERB agonists have no effect. These results indicate that pharmacological targeting of REV-ERB …

The Influence Of National Culture On Effectiveness Of Safety Trainings During Postdisaster Reconstruction, Behzad Esmaeili, Kevin Grosskopf, Amy Javernick-Will

Durham School of Architectural Engineering and Construction: Faculty Publications

Non-English speaking workers constitute a disproportionately high number of workers involved in postdisaster reconstruction. Additionally, the rate of fatality among these workers is higher than the industry average. Research shows this population is more prone to unsafe behaviors in the working environment, conceivably because many of these workers are sent into the field prior to any formalized training. Recent studies show that the native culture of construction workers can impact risk-taking behavior. While numerous researchers have attempted to develop training materials for Hispanic workers, the number of studies that consider the impact of native culture on safety behavior is minimal. …

Quorum Sensing Activity And Control Of Yeast-Mycelium Dimorphism In Ophiostoma Floccosum, Alexander Berrocal, Claudia Oviedo, Kenneth W. Nickerson, José Navarrete

School of Biological Sciences: Faculty Publications

Quorum sensing (QS) activity in Ophiostoma fungi has not been described. We have examined the growth conditions on the control of dimorphism in Ophiostoma floccosum, an attractive biocontrol agent against blue-stain fungi, and its relationship with QS activity. In a defined culture medium with l-proline as the N source, a high inoculum size (10⁷ c.f.u. ml^-1) was the principal factor that promoted yeast-like growth. Inoculum size effect can be explained by the secretion of a QS molecule(s) (QSMs) responsible for inducing yeast morphology. QSM candidates were extracted from spent medium and their structure was determined by GC–MS. …

Comparative Analyses Of Three Chlorella Species In Response To Light And Sugar Reveal Distinctive Lipid Accumulation Patterns In The Microalga C. Sorokiniana, Julian N. Rosenberg, Naoko Kobayashi, Austin Barnes, Eric A. Noel, Michael J. Betenbaugh, George A. Oyler

Department of Biochemistry: Faculty Publications

While photosynthetic microalgae, such as Chlorella, serve as feedstocks for nutritional oils and biofuels, heterotrophic cultivation can augment growth rates, support high cell densities, and increase triacylglycerol (TAG) lipid content. However, these species differ significantly in their photoautotrophic and heterotrophic characteristics. In this study, the phylogeny of thirty Chlorella strains was determined in order to inform bioprospecting efforts and detailed physiological assessment of three species. The growth kinetics and lipid biochemistry of C. protothecoides UTEX 411, C. vulgaris UTEX 265, and C. sorokiniana UTEX 1230 were quantified during photoautotrophy in Bold’s basal medium (BBM) and heterotrophy in BBM supplemented …

Plant Acyl-Coa:Lysophosphatidylcholine Acyltransferases (Lpcats) Have Different Specificities In Their Forward And Reverse Reactions, Ida Lager, Jenny Lindberg Yilmaz, Xue-Rong Zhou, Katarzyna Jasieniecka, Michael Kazachkov, Peng Wang, Jitao Zou, Randall Weselake, Mark A. Smith, Shen Bayon, John M. Dyer, Jay Shockey, Ernst Heinz, Allan Green, Antoni Banas, Sten Stymne

Department of Biochemistry: Faculty Publications

Background: Acyl-CoA:lysophosphatidylcholine acyltransferase (LPCAT) enzymes have central roles in acyl editing of phosphatidylcholine.

Results: Plant LPCATs were expressed in yeast and biochemically characterized.

Conclusion: LPCATs can edit acyl composition of phosphatidylcholine through their combined forward and reverse reactions.

Significance: Plant LPCATs play a role in editing both sn-positions of PC and remove ricinoleic acid with high selectivity from this lipid.

Emerging Regulatory Paradigms In Glutathione Metabolism, Yilin Liu, Annastasia S. Hyde, Melanie A. Simpson, Joseph J. Barycki

Department of Biochemistry: Faculty Publications

One of the hallmarks of cancer is the ability to generate and withstand unusual levels of oxidative stress. In part, this property of tumor cells is conferred by elevation of the cellular redox buffer glutathione. Though enzymes of the glutathione synthesis and salvage pathways have been characterized for several decades, we still lack a comprehensive understanding of their independent and coordinate regulatory mechanisms. Recent studies have further revealed that overall central metabolic pathways are frequently altered in various tumor types, resulting in significant increases in biosynthetic capacity, and feeding into glutathione synthesis. In this review, we will discuss the enzymes …

Hepatoprotective Role Of Sestrin2 Against Chronic Er Stress, Hwan-Woo Park, Haeli Park, Seung-Hyun Ro, Insook Jang, Ian A. Semple, David N. Kim, Myungjin Kim, Myeongjin Nam, Deqiang Zhang, Lei Yin, Jun Hee Lee

Department of Biochemistry: Faculty Publications

Upon prolonged endoplasmic reticulum (ER) stress, cells attenuate protein translation to prevent accumulation of unfolded proteins. Here we show that Sestrin2 is critical for this process. Sestrin2 expression is induced by an ER stress-activated transcription factor CCAATenhancer- binding protein beta (c/EBPβ). Once induced, Sestrin2 halts protein synthesis by inhibiting mammalian target of rapamycin complex 1 (mTORC1). As Sestrin2-deficient cells continue to translate a large amount of proteins during ER stress, they are highly susceptible to ER stress-associated cell death. Accordingly, dietary or genetically induced obesity, which does not lead to any pathological indication other than simple fat accumulation in the …

Effect Of Prey Richness On A Consumer’S Intrinsic Growth Rate, Brian J. Darby, Michael A. Herman

School of Biological Sciences: Faculty Publications

The intrinsic growth rate of nonselective microbivores increases asymptotically with increasing prey biomass, but we do not know how intrinsic growth rate is affected by prey richness. The objective of this experiment was to determine the effect of prey richness on the growth kinetics of nematode predators while grazing on mixed bacterial lawns. We found that the intrinsic growth rate of Caenorhabditis elegans in laboratory culture increased asymptotically with prey richness. The mechanism of this pattern was primarily due to the best available prey species in the mixture: the intrinsic growth rate of the consumer feeding on a mixture of …

Variation In The Local Population Dynamics Of The Short-Lived Opuntia Macrorhiza (Cactaceae), Chirakkal V. Haridas, Kathleen H. Keeler, Brigitte Tenhumberg

School of Biological Sciences: Faculty Publications

Spatio-temporal variation in demographic rates can have profound effects for population persistence, especially for dispersal-limited species living in fragmented landscapes. Long-term studies of plants in such habitats help with understanding the impacts of fragmentation on population persistence but such studies are rare. In this work we reanalyzed demographic data from seven years of the short-lived cactus Opuntia macrorhiza var. macrorhiza at five plots in Boulder, Colorado. Previous work combining data from all years and all plots predicted a stable population (deterministic log λ ≈ 0). This approach assumed that all five plots were part of a single population. Since the …

The Body-Size Dependence Of Mutual Interference, John P. Delong

School of Biological Sciences: Faculty Publications

The parameters that drive population dynamics typically show a relationship with body size. By contrast, there is no theoretical or empirical support for a body-size dependence of mutual interference, which links foraging rates to consumer density. Here, I develop a model to predict that interference may be positively or negatively related to body size depending on how resource body size scales with consumer body size. Over a wide range of body sizes, however, the model predicts that interference will be body-size independent. This prediction was supported by a new data set on interference and consumer body size. The stabilizing effect …

Leaky Termination At Premature Stop Codons Antagonizes Nonsense-Mediated Mrna Decay In S. Cerevisiae, Kim M. Keeling, Jessica Lanier, Ming Du, Joe Salas-Marco, Lin Gao, Anisa Kaenjak-Angeletti, David M. Bedwell

School of Biological Sciences: Faculty Publications

The Nonsense-Mediated mRNA Decay (NMD) pathway mediates the rapid degradation of mRNAs that contain premature stop mutations in eukaryotic organisms. It was recently shown that mutations in three yeast genes that encode proteins involved in the NMD process, UPF1, UPF2, and UPF3, also reduce the efficiency of translation termination. In the current study, we compared the efficiency of translation termination in a upf∆ strain and a [PSI⁺] strain using a collection of translation termination reporter constructs. The [PSI⁺] state is caused by a prion form of the polypeptide chain release factor eRF3 that limits its availability …

Saturated Free Fatty Acids Induce Cholangiocyte Lipoapoptosis, Sathish Kumar Natarajan, Sally A. Ingham, Ashley M. Mohr, Cody J. Wehrkamp, Anuttoma Ray, Sohini Roy, Sophie C. Cazanave, Mary Anne Phillippi, Justin L. Mott

Department of Biochemistry: Faculty Publications

Recent studies have identified a cholestatic variant of nonalcoholic fatty liver disease (NAFLD) with portal inflammation and ductular reaction. Based on reports of biliary damage, as well as increased circulating free fatty acids (FFAs) in NAFLD, we hypothesized the involvement of cholangiocyte lipoapoptosis as a mechanism of cellular injury. Here, we demonstrate that the saturated FFAs palmitate and stearate induced robust and rapid cell death in cholangiocytes. Palmitate and stearate induced cholangiocyte lipoapoptosis in a concentration-dependent manner in multiple cholangiocyte-derived cell lines. The mechanism of lipoapoptosis relied on the activation of caspase 3/7 activity. There was also a significant up-regulation …

Nutrient Composition Of Selected Traditional United States Northern Plains Native American Plant Foods, Katherine M. Phillips, Pamela R. Pehrsson, Wanda W. Agnew, Angela J. Scheett, Jennifer R. Follett, Henry C. Lukaski, Kristine Y. Patterson

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Ten wild plants (cattail broad leaf shoots, chokecherries, beaked hazelnuts, lambsquarters, plains prickly pear, prairie turnips, stinging nettles, wild plums, raspberries, and rose hips) from three Native American reservations in North Dakota were analyzed to expand composition information of traditional foraged plants. Proximates, dietary fiber (DF), vitamins, minerals, carotenoids, and folate vitamers were assayed using standard methods and reference materials. Per serving, all were rich in Mn (100–2808 mg). Several provided >10% DRI of Fe (cattail shoots, steamed lambsquarters, and prairie turnips), Ca (steamed lambsquarters, prickly pear, and prairie turnips), Mg (cattail shoots, lambsquarters, prickly pear, and prairie turnips), vitamins …

Litters Of Photosynthetically Divergent Grasses Exhibit Differential Metabolic Responses To Warming And Elevated Co2, Vidya Suseela, Daniella Triebwasser-Freese, Nora Linscheid, Jack A. Morgan, Nishanth Tharayil

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Climatic stress such as warming would alter physiological pathways in plants leading to changes in tissue chemistry. Elevated CO₂ could partly mitigate warming induced moisture stress, and the degree of this mitigation may vary with plant functional types. We studied the composition of structural and non-structural metabolites in senesced tissues of Bouteloua gracilis (C4) and Pascopyrum smithii (C3) at the Prairie Heating and CO₂ Enrichment experiment, Wyoming, USA. We hypothesized that P. smithii and B. gracilis would respond to unfavorable global change factors by producing structural metabolites and osmoregulatory compounds that are necessary to combat stress. However, due …

2014 Nebraska Beef Cattle Report -- Frontmatter & Contents

Nebraska Beef Cattle Reports

Title page

Acknowledgments

Table of Contents