Digital Commons Network^™

Complete Genome Sequence Of Uv-Resistant Campylobacter Jejuni Rm3194, Including An 81.08-Kilobase Plasmid, Nereus W. Gunther Iv, Erin R. Reichenberger, James L. Bono

Roman L. Hruska U.S. Meat Animal Research Center: Reports

Annually, Campylobacter spp. are responsible for the greatest number of foodborne gastrointestinal bacterial infections in the developed world (1, 2). However, Campylobacter spp. are nutritionally fastidious organisms requiring a microaerobic environment for survival (3). It remains uncertain by what mechanisms Campylobacter survives within multiple hostile environments in sufficient numbers to cause such significant amounts of human disease.

Campylobacter jejuni strain RM3194 was isolated from a clinical sample in 1994 from a human enteritis case at the Red Cross War Memorial Children’s Hospital in Cape Town, South Africa, and was supplied to our laboratory by Robert Mandrell (ARS, Albany, CA) (4, …

Annual Report: Nebraska Extension In Lancaster County, January 1, 2016 Through December 31, 2016

Lancaster County, Nebraska Extension: Reports

Nebraska Extension accomplishes University of Nebraska- Lincoln 's landgrant mission by extending reliable, research-based information to the public for use in everyday life. It provides programs and resources in the areas of horticulture, pest management, nutrition & food safety, farms & acreages, strong families, youth development, environment of the home, and leadership development.

Agroforestry Horizons: Experiences And Reflections: Report-Out, Steve Rasmussen

United States Department of Agriculture, Forest Service / University of Nebraska-Lincoln: Faculty Publications

The USDA National Agroforestry Center (NAC) is charting a course for the next five years. As part of this process, NAC and the USDA Interagency Agroforestry Team hosted a meeting of leaders in the fields of agroforestry, agriculture, and forestry, from government, universities, and the private sector, to share experiences and insights on agroforestry opportunities and challenges. The meeting occurred on April 27th and 28th, 2016, in Washington, DC, and entailed presentations and discussions on current and potential roles of agroforestry in supporting water quality, soil health, economic diversification, food security, and pollinator/wildlife habitat. This report-out provides a summary of …

Voxel-Based Morphometry Reveals Brain Gray Matter Volume Changes In Successful Dieters, Robyn A. Honea, Amanda N. Szabo-Reed, Rebecca J. Lepping, Rodrigo Perea, Florence Breslin, Laura E. Martin, William M. Brooks, Joseph E. Donnelly, Cary R. Savage

Center for Brain, Biology, and Behavior: Faculty Publications

Objective: To compare regional brain volume predictors of percent weight loss (WL) in dieters with obesity (DwO) and in the same participants categorized as “successful” (≥7% WL) or “unsuccessful” dieters (

Limited Mitogenomic Degradation In Response To A Parasitic Lifestyle In Orobanchaceae, Weishu Fan, Andan Zhu, Melisa Kozaczek, Neethu Shah, Natalia Pabón-Mora, Favio González, Jeffrey P. Mower

Center for Plant Science Innovation: Faculty Publications

In parasitic plants, the reduction in plastid genome (plastome) size and content is driven predominantly by the loss of photosynthetic genes. The first completed mitochondrial genomes (mitogenomes) from parasitic mistletoes also exhibit significant degradation, but the generality of this observation for other parasitic plants is unclear. We sequenced the complete mitogenome and plastome of the hemiparasite Castilleja paramensis (Orobanchaceae) and compared them with additional holoparasitic, hemiparasitic and nonparasitic species from Orobanchaceae. Comparative mitogenomic analysis revealed minimal gene loss among the seven Orobanchaceae species, indicating the retention of typical mitochondrial function among Orobanchaceae species. Phylogenetic analysis demonstrated that the mobile cox1 …

Identification Of Homogentisate Dioxygenase As A Target For Vitamin E Biofortification In Oilseeds, Minviluz G. Stacey, Rebecca E. Cahoon, Hanh T. Nguyen, Yaya Cui, Shirley Sato, Cuong T. Nguyen, Nongnat Phoka, Kerry M. Clark, Yan Liang, Joe Forrester, Josef Batek, Phat Tien Do, David A. Sleper, Thomas E. Clemente, Edgar B. Cahoon, Gary Stacey

Center for Plant Science Innovation: Faculty Publications

Soybean (Glycine max) is a major plant source of protein and oil and produces important secondary metabolites beneficial for human health. As a tool for gene function discovery and improvement of this important crop, a mutant population was generated using fast neutron irradiation. Visual screening of mutagenized seeds identified a mutant line, designated MO12, which produced brown seeds as opposed to the yellow seeds produced by the unmodified Williams 82 parental cultivar. Using forward genetic methods combined with comparative genome hybridization analysis, we were able to establish that deletion of the GmHGO1 gene is the genetic basis of …

The Ecological Role Of Marine Mammal-Eating Killer Whales In The North Pacific Ocean Surrounding Alaska

United States Marine Mammal Commission

No abstract provided.

Getting Farming On The Agenda: Planning, Policymaking, And Governance Practices Of Urban Agriculture In New York City, Lindsay K. Campbell

United States Department of Agriculture, Forest Service / University of Nebraska-Lincoln: Faculty Publications

How and why is urban agriculture taken up into local food policies and sustainability plans? This paper uses a case study of urban agriculture policymaking in New York City from 2007 to 2011 to examine the power-laden operation of urban environmental governance. It explores several ‘faces of power,’ including overt authority, institutionalized ‘rules of the game,’ and hegemony. It also investigates how multiple actors interact in policymaking processes, including through the construction and use of broad discursive concepts. Findings draw upon analysis of policy documents and semi-structured interviews with 43 subjects engaged in food systems policymaking. Some municipal decision-makers questioned …

Size Difference In Whooping Cranes Reared For Two Reintroduction Methods, Richard P. Urbanek, Marianne M. Wellington, Sabrina Servanty

Proceedings of the North American Crane Workshop

We investigated a possible size difference in whooping cranes (Grus americana) captive-reared for 2 reintroduction methods to establish a migratory population in eastern North America. Cranes reared for ultralight aircraft-led migration (UL) to Florida were significantly larger than cranes reared for direct autumn release (DAR) on the natal area in central Wisconsin. Mean tarsal length was 315.5 ± 0.98 (1 SE) and 308.1 ± 1.87 mm, respectively, for UL and DAR males and 296.9 ± 1.03 and 290.8 ± 2.60 mm, respectively, for UL and DAR females. Because of the different rearing schedules, eggs for the DAR method …

Evaluation Of A Nutraceutical Joint Supplement In Cranes, Kendra L. Bauer, Ellen S. Dierenfeld, Barry K. Hartup

Proceedings of the North American Crane Workshop

Osteoarthritis is a problem that threatens the reproductive capabilities of captive populations of endangered cranes. In our pilot study, we used 5 cranes with a history of unilateral, chronic tarsal pathology in a cross-over design to gauge the effects of the primary ingredient (NEM®, ESM Technologies LLC, Carthage, MO) of the nutraceutical Steadfast® (Novus Nutrition Brands, LLC, St. Charles, MO). We evaluated the ingredient for acceptance, safety, and short-term efficacy. To evaluate efficacy, we collected goniometric measures to determine range of motion in each tarsal joint before and after a 5-week experimental period where NEM® was offered in pelleted feed. …

Serum Chemistry, Blood Gas, And Physiological Measures Of Sandhill Cranes Sedated With Alpha-Chloralose, Richard R. Sim, Barry K. Hartup

Proceedings of the North American Crane Workshop

Capture techniques that lessen handling stress may also lessen pathologic influences on physiologic measures, improving the validity of these measures for use in individual health assessment of freeranging wildlife. Since 1990, the International Crane Foundation (ICF) has successfully used chemical immobilization with alpha-chloralose (AC; C₆H₁₁Cl₃O₆), a chloral derivative of glucose, to facilitate captures of sandhill cranes (Grus canadensis tabida) for ecological studies (Hayes et al. 2003). Although this chemical has been used orally for the immobilization of many species, the physiologic effects of AC are not well understood in cranes. …

Proceedings Of The 13th North American Crane Workshop Abstracts

Proceedings of the North American Crane Workshop

AGE-SPECIFIC SURVIVAL OF GREATER SANDHILL CRANE COLTS IN NEVADA Chad August, Jim Sedinger, and Chris Nicolai 111

WHOOPING CRANE DISTRIBUTION AND HABITAT USE: PAST, PRESENT, AND FUTURE? Jane Austin, Matthew Hayes, and Jeb Barzen 111

BEHAVIORAL MOVEMENTS OF ARANSAS-WOOD BUFFALO WHOOPING CRANES: ANOMALIES OR INDICATIONS OF WAYS TO FURTHER ENHANCE SPECIES RECOVERY. David Baasch, Mark Bidwell, Wade Harrell, Kris Metzger, Aaron Pearse, and Mary Harner 112

ON NEST SUCCESS IN A REINTRODUCED POPULATION OF WHOOPING CRANES .Jeb Barzen, Sarah Converse, Peter Adler, Elmer Gray, Anne Lacy, Eva Szyszkoski, and Andrew Gossens 113

DETERMINING DIET COMPOSITION AND INGESTION RATE OF CRANES …

Proceedings Of The Thirteenth North American Crane Workshop, 14–17 April 2014, Lafayette, Louisiana, Usa, David A. Aborn, Richard P. Urbanek

Proceedings of the North American Crane Workshop

RESEARCH PAPERS

HABITAT SELECTION BY BREEDING SANDHILL CRANES IN CENTRAL WISCONSIN. Tamara P. Miller and Jeb A. Barzen 1

HIGH NEST DENSITY OF SANDHILL CRANES IN CENTRAL WISCONSIN.Jeb A. Barzen, Liying Su, Anne E. Lacy, Andrew P. Gossens, and Dorn M. Moore 13

DIFFERENTIAL DETECTION OF TERRITORIAL AND NON-TERRITORIAL GREATER SANDHILL CRANES IN SUMMER. Lindsey F. McKinney, Jeb A. Barzen, Jason D. Riddle, Shelli A. Dubay, and Tim F. Ginnett 25

TIMING OF FAMILY DISSOCIATION DOES NOT AFFECT LONG-TERM SURVIVAL ESTIMATES OF SANDHILL CRANE CHICKS Matthew A. Hayes and Jeb A. Barzen 33

A REVIEW OF FALL SANDHILL CRANE MIGRATION …

Improving Outcomes For Infants With Single Ventricle Physiology Through Standardized Feeding During The Interstage, Cindy Weston, S. Adil Husain, Christopher L. Curzon, Steve Neish, Gemma T. Kennedy, Krista Bonagurio, Kevin Gosselin

College of Nursing--Lincoln Campus: Faculty Publications

Congenital heart disease is identified as the most common birth defect with single ventricle physiology carrying the highest mortality. Staged surgical palliation is required for treatment, with mortality historically as high as 22% in the four- to six-month period from the first- to second-stage surgical palliation, known as the interstage. A standardized postoperative feeding approach was implemented through an evidence-based protocol, parent engagement, and interprofessional team rounds. Five infants with single ventricle physiology preprotocol were compared with five infants who received the standardized feeding approach. Mann- Whitney 𝑈 tests were conducted to evaluate the hypotheses that infants in the intervention …

A Urinary Metabolic Signature For Multiple Sclerosis And Neuromyelitis Optica, Teklab Gebregiworgis, Helle H. Nielsen, Chandirasegara Massilamany, Arunakumar Gangaplara, Jay Reddy, Zsolt Illes, Robert Powers

Jay Reddy Publications

Urine is a metabolite-rich biofluid that reflects the body’s effort to maintain chemical and osmotic homeostasis. Clinical diagnosis routinely relies on urine samples because the collection process is easy and noninvasive. Despite these advantages, urine is an under-investigated source of biomarkers for multiple sclerosis (MS). Nuclear magnetic resonance spectroscopy (NMR) has become a common approach for analyzing urinary metabolites for disease diagnosis and biomarker discovery. For illustration of the potential of urinary metabolites for diagnosing and treating MS patients, and for differentiating between MS and other illnesses, 38 urine samples were collected from healthy controls, MS patients, and neuromyelitis optica-spectrum …

The Influence Of A Severe Reservoir Drawdown On Springtime Zooplankton And Larval Fish Assemblages In Red Willow Reservoir, Nebraska, Jason A. Deboer, Christa M. Webber, Taylor A. Dixon, Kevin L. Pope

Nebraska Cooperative Fish and Wildlife Research Unit: Staff Publications

Reservoirs can be dynamic systems, often prone to unpredictable and extreme waterlevel fluctuations, and can be environments where survival is difficult for zooplankton and larval fish. Although numerous studies have examined the effects of extreme reservoir drawdown on water quality, few have examined extreme drawdown on both abiotic and biotic characteristics. A fissure in the dam at Red Willow Reservoir in southwest Nebraska necessitated an extreme drawdown; the water level was lowered more than 6 m during a two-month period, reducing reservoir volume by 76%. During the subsequent low-water period (i.e., post-drawdown), spring sampling (April-June) showed dissolved oxygen concentration was …

Nebraska Annual Social Indicators Survey (Nasis) 2015-2016 Methodology Report, Bureau Of Sociological Research

Nebraska Annual Social Indicators Survey (NASIS)

Bureau of Sociological Research

2015-2016 NASIS Methodology Report

Contents

Introduction 3

Mode Selection 3

Design and Item Selection 3

Sampling Design 4

Experimental Design Treatment 4

Data Collection Process 4

Response Rate 5

Data-Entry Training, Supervision, and Quality Control 5

Processing of Completed Surveys 5

Data Cleaning 5

NASIS Sample Weights 6

Design Effects 6

Questions 6

Estimate of Sampling Error 9

Appendices 10

Appendix A: Cover Letters 10

First Mailing Cover Letter 10

Second Mailing Cover Letter 11

Appendix B: Survey Instrument 12

Version 1 (printed in black & white only) 12

Version 2 (printed in color) 28

Version …

Nasis 2016: Nebraska Annual Social Indicators Survey Questionnaire, Bureau Of Sociological Research

Nebraska Annual Social Indicators Survey (NASIS)

We need your help to learn about how Nebraskans think, feel, and live. Researchers from the University of Nebraska and across the state are counting on your help to learn about a variety of issues. Your responses will help shape program and policy development in Nebraska now and into the future.

127 questions; 16 pages

Working To Ensure A Water And Food Secure World Annual Report Fy2016 (July 1, 2015 - June 30, 2016), Robert B. Daugherty Water For Food Global Institute

Daugherty Water for Food Global Institute: Administrative Materials

From rural Nebraska to rural Tanzania, the Water for Food Global Institute is working to achieve greater food security with less pressure on the planet’s scarce water resources, fueling change through conversations, collaborations and on-the-ground impacts. Leveraging Leadership, Partnerships and Resources for Global Impact The Water for Food Global Institute is a vehicle for collaboration, operating through a network of constituent parts that leverages the leadership, resources and rich history of experience and expertise at the University of Nebraska. In addition to our core group of staff, governed by a board of directors and with guidance from internal and external …

Weight Control Practices Of Division I National Collegiate Athletic Association Athletes, Patrick B. Wilson, Leilani Madrigal, Judith M. Burnfield

Athletic Performance Research

Objectives: Altering body weight can have substantial effects on an athlete’s performance and well-being. Limited information is available describing the weight control practices of Division I National Collegiate Athletic Association (NCAA) athletes.

Methods: Weight control practices data from 188 (138 male and 50 female; 18-23 y) Division I NCAA athletes were analyzed as a part of this cross-sectional, retrospective study. Participants completed questionnaires on weight control practices and weight control nutrition knowledge at the end of their season and were classified into weight-sensitive and less weight-sensitive sports.

Results: A higher proportion of females attempted to lose weight than males among …

Drought And Health In The Context Of Public Engagement, Nicole Wall, Michael Hayes

National Drought Mitigation Center: Faculty Publications

Droughts have profoundly affected societies around the world from the earliest beginnings. A recent estimate from the Centre for Research on the Epidemiology of Disasters (CRED) claims that more than 1 billion people have been affected by drought during the twenty-year period between 1994 and 2013. Because of the characteristics of drought, drought impacts are often difficult to identify and quantify, and this is especially true with public health-oriented drought consequences, including those resulting from low water quantities, poor water quality, mental health and stress, dust and windblown agents, and wildlife intrusion. However, when officials emphasize adopting a proactive risk …

Block And Bridle Annual 2016

Block and Bridle Annual (1933–2021)

Three objectives of the National Block and Bridle Club are to promote animal agriculture through the activities of the club, to supplement students’ study of the animal sciences, and to bring about a closer relationship among students who choose to be a part of the organization. I believe the Block and Bridle club at the University of Nebraska - Lincoln has been successful in accomplishing these goals over the past year and I sincerely thank all of the officers and members who have given their time and effort to make this possible. I am excited to say that our active …

The Association Of Urinary Polycyclic Aromatic Hydrocarbon Biomarkers And Cardiovascular Disease In The Us Population, Omayma Alshaarawy, Hosam A. Elbaz, Michael E. Andrew

United States Public Health Resources

Background: Polycyclic aromatic hydrocarbons (PAHs) are potent atmospheric pollutants produced by incomplete combustion of organic materials. Pre-clinical and occupational studies have reported a positive association of PAHs with oxidative stress, inflammation and subsequent development of atherosclerosis, a major underlying risk factor for cardiovascular disease (CVD). The aim of the current study is to estimate the association between levels of PAH biomarkers and CVD in a national representative sample of United States (US) adults.

Methods: We examined adult participants (≥20 years of age) from the merged US National Health and Nutrition Examination Survey 2001–2010. Logistic regression models were used to estimate …

Assessing Climate Change And Health Vulnerability At The Local Level: Travis County, Texas, Natasha Prudent, Adele Houghton, George Luber

United States Public Health Resources

We created a measure to help comprehend population vulnerability to potential flooding and excessive heat events using health, built environment and social factors. Through principal component analysis (PCA), we created non-weighted sum index scores of literature-reviewed social and built environment characteristics. We created baseline poor health measures using 1999–2005 ageadjusted cardiovascular and combined diabetes and hypertension mortality rates to correspond with social–built environment indices. We mapped US Census block groups by linked ageadjusted mortality and a PCA-created social–built environment index. The goal was to measure flooding and excessive heat event vulnerability as proxies for population vulnerability to climate change for …

Urinary Concentrations Of Pah And Voc Metabolites In Marijuana Users, Binnian Wei, K. Udeni Alwis, Zheng Li, Lanqing Wang, Liza Valentin-Blasini, Connie S. Sosnoff, Yang Xia, Kevin P. Conway, Benjamin C. Blount

United States Public Health Resources

Background: Marijuana is seeing increased therapeutic use, and is the world's third most-popular recreational drug following alcohol and tobacco. This widening use poses increased exposure to potentially toxic combustion by-products from marijuana smoke and the potential for public health concerns.

Objectives: To compare urinary metabolites of polycyclic aromatic hydrocarbons (PAHs) and volatile organic compounds (VOCs) among self-reported recent marijuana users and nonusers, while accounting for tobacco smoke exposure.

Methods: Measurements of PAH and VOC metabolites in urine samples were combined with questionnaire data collected from participants in the National Health and Nutrition Examination Surveys (NHANES) from 2005 to 2012 in …

Cell Non-Autonomous Regulation Of Hepatic Igf-1 And Neonatal Growth By Kinase Suppressor Of Ras 2 (Ksr2), Lili Guo, Diane L. Costanzo-Garvey, Deandra R. Smith, Megan E. Zavorka, Megan Venable-Kang, Richard G. Macdonald, Robert E. Lewis

United States Public Health Resources

Individuals with poor postnatal growth are at risk for cardiovascular and metabolic problems as adults. Here we show that disruption of the molecular scaffold Kinase Suppressor of Ras 2 (KSR2) causes selective inhibition of hepatic GH signaling in neonatal mice with impaired expression of IGF-1 and IGFBP3. ksr2^−/− mice are normal size at birth but show a marked increase in FGF21 accompanied by reduced body mass, shortened body length, and reduced bone mineral density (BMD) and content (BMC) first evident during postnatal development. However, disrupting FGF21 in ksr2^−/− mice does not normalize mass, length, or bone density and …

Feeding Wet Distillers Grains Plus Solubles With And Without A Direct-Fed Microbial To Determine Performance, Carcass Characteristics, And Fecal Shedding Of Escherichia Coli O157:H7 In Feedlot Heifers, B. K. Wilson, B. P. Pulsipher, D. L. Step, M. E. Jacob, D. L. Vanoverbeke, C. J. Richards, T. G. Nagaraja, C. R. Krehbiel

Department of Animal Science: Faculty Publications

The inclusion of wet distillers grains plus solubles (WDGS) in feedlot diets has become a common practice in many regions of the United States due to the expanded production of byproducts and fluctuating corn prices related to ethanol production and other factors. In addition, societal concerns over the continued use of antimicrobials in agriculture production combined with an enhanced interest in disease and pathogen prevention in the food supply have led to an increased interest in use of direct-fed microbials (DFM) in growing and finishing cattle. Direct-fed microbials have been shown to improve ADG and feed efficiency, alter ruminal fermentation, …

2016 Extension Highlights

Lancaster County, Nebraska Extension: Reports

Nebraska Extension is your local connection to university research-based information. We extend knowledge, provide solutions and change lives. Nebraska Extension in Lancaster County is a partnership between the University of Nebraska–Lincoln, Lancaster County and the United States Department of Agriculture.

We extend knowledge, provide solutions and change lives in these educational program areas:
• Cropping & Water Systems
• Beef Systems
• Food, Nutrition & Health
• Community Environment
• Learning Child
• 4-H Youth Development
• Community Vitality

Here’s a look at some of Nebraska Extension in Lancaster County’s 2016 impacts.

Nutrigenomic Functions Of Ppars In Obesogenic Environments, Soonkyu Chung, Young Jun Kim, Soo Jin Yang, Yunkyoung Lee, Myoungsook Lee

Department of Nutrition and Health Sciences: Faculty Publications

Peroxisome proliferator-activated receptors (PPARs) are ligand-activated transcription factors that mediate the effects of several nutrients or drugs through transcriptional regulation of their target genes in obesogenic environments.This review consists of three parts. First, we summarize current knowledge regarding the role of PPARs in governing the development of white and brown/beige adipocytes from uncommitted progenitor cells. Next, we discuss the interactions of dietary bioactive molecules, such as fatty acids and phytochemicals, with PPARs for the modulation of PPAR-dependent transcriptional activities and metabolic consequences. Lastly, the effects of PPAR polymorphism on obesity and metabolic outcomes are discussed. In this review, we aim …

Glucagon Regulates Hepatic Lipid Metabolism Via Camp And Insig-2 Signaling: Implication For The Pathogenesis Of Hypertriglyceridemia And Hepatic Steatosis, Hai Wang, Miaoyun Zhao, Neetu Sud, Patricia Christian, Jing Shen, Yongyan Song, Anjeza Pashaj, Kezhong Zhang, Timothy P. Carr, Qiaozhu Su

Department of Nutrition and Health Sciences: Faculty Publications

Insulin induced gene-2 (Insig-2) is an ER-resident protein that inhibits the activation of sterol regulatory element-binding proteins (SREBPs). However, cellular factors that regulate Insig-2 expression have not yet been identified. Here we reported that cyclic AMP-responsive element-binding protein H (CREBH) positively regulates mRNA and protein expression of a liver specific isoform of Insig-2, Insig-2a, which in turn hinders SREBP-1c activation and inhibits hepatic de novo lipogenesis. CREBH binds to the evolutionally conserved CRE-BP binding elements located in the enhancer region of Insig-2a and upregulates its mRNA and protein expression. Metabolic hormone glucagon and nutritional fasting activated CREBH, which upregulated expression …