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Nebline, September 2019

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

Read for Resilience Program Helps Young Children Cope After a Disaster

Breakfast — Don’t Leave Home Without It!

Recipe of the Month

Grazing Calves on Annual Forages

Sheet Composting Requires No Turning

Composting Demonstrations

Prepared, Not Scared. Be Ready for Disasters

Plant Asters for Fall Color

Garden Guide: Things to Do This Month

The Lowdown on Lyme Disease

Heart of 4-H Volunteer Award: Brenda Nepper

4-H Teen Council Invites New Members

Support 4-H by Dining at Chipotle on O Street, Sept. 16

New Horticulture 4-H Club Meeting, Sept. 19

4-H Golf Tournament Fundraiser, Sept. 29

Interested in Joining 4-H? Come …

Steering Science Through Output Indicators And Data Capitalism, Ulrich Herb

Copyright, Fair Use, Scholarly Communication, etc.

Since the mid-1920s, a scientist's performance was tracked by using bibliometric information such as the number of publications or their citations. Today, there are unprecedented possibilities for controlling science by analysing data on production and use of scientific information, so that citations only play a subordinate role in the evaluation of science. To illustrate this change, we take a look at the history of the Dutch publishing house Elsevier and its metamorphosis into a research intelligence service provider, because it ideally reflects the new possibilities of logging and controlling science.

Predicting Functional Responses In Agro-Ecosystems From Animal Movement Data To Improve Management Of Invasive Pests, Mark Q. Wilber, Sarah M. Chinn, James C. Beasley, Raoul K. Bourghton, Ryan K. Brooks, Stephen S. Ditchkoff, Justin W. Fischer, Steve B. Hartley, Lindsey K. Holmstrom, John C. Kilgo, Jesse S. Lewis, Ryan S. Miller, Nathan P. Snow, Kurt C. Vercauteren, Samantha M. Wisely, Colleen T. Webb, Kim M. Peen

United States Department of Agriculture Wildlife Services: Staff Publications

Functional responses describe how changing resource availability affects consumer resource use, thus providing a mechanistic approach to prediction of the invasibility and potential damage of invasive alien species (IAS). However, functional responses can be context dependent, varying with resource characteristics and availability, consumer attributes, and environmental variables. Identifying context dependencies can allow invasion and damage risk to be predicted across different ecoregions. Understanding how ecological factors shape the functional response in agro-ecosystems can improve predictions of hotspots of highest impact and inform strategies to mitigate damage across locations with varying crop types and availability. We linked heterogeneous movement data across …

The Impact Of Responsive Feeding Practice Training On Teacher Feeding Behaviors In Tribal Early Care And Education: The Fresh Study, Kaysha Sleet, Susan B. Sisson, Dipti Dev, Charlotte Love, Mary B. Williams, Leah A. Hoffman, Valarie Blue Bird Jernigan

Department of Child, Youth, and Family Studies: Faculty Publications

Background: Establishing healthy eating habits early impacts lifelong dietary intake, which has implications for many health outcomes. With children spending time in early care and education (ECE) programs, teachers establish the daytime meal environment through their feeding practices.

Objective: To determine the effect of a teacher-focused intervention to increase responsive feeding practices in two interventions, one focused exclusively on the teacher’s feeding practices and the other focused on both the teacher’s feeding practices and a nutrition classroom curriculum in ECE teachers in a Native American (NA) community in Oklahoma.

Methods: Nine tribally-affiliated ECE programs were randomly assigned to an intervention: …

Role Of Dietary Flavonoid Compounds In Driving Patterns Of Microbial Community Assembly, Kerry L. Ivey, Andrew T. Chan, Jacques Izard, Aedin Cassidy, Geraint B. Rogers, Eric B. Rimm

Department of Food Science and Technology: Faculty Publications

Flavonoids are a group of polyphenolic dietary compounds found in many different plant-based foods. There is increasing evidence that higher flavonoid intake may be causally linked to a reduced risk of cardiovascular disease and other chronic diseases. The bioactivity and bioavailability of many dietary flavonoids can be influenced by gastrointestinal microbiome metabolism. However, the role that habitual flavonoid intake plays in shaping the human gut microbiome is poorly understood. We describe an application of an ecosystem-based analytic approach to nutritional, microbiome, and questionnaire data from a cohort of more than 240 generally healthy adult males to assess the role of …

Occupied, Judy Diamond, Tom Floyd, Rebecca Smith, Ann Downer-Hazell, Martin Powell, Nick Poliwko, Angie Fox, Amy Spiegel, Patricia Wonch Hill, Julia Mcquillan

Zea E-Books Collection

Our bodies are home to more microbes than human cells. The balance of helpful to harmful microbes in our bodies can make us sick or healthy. The Biology of Human project focuses on helping people understand themselves by exploring scientific principles that underlie modern research in human biology. Biology of Human is an alliance of science educators, artists, science writers, and biomedical researchers working to increase public understanding about viruses and infectious disease. In this comic, Daniel and Miguel find themselves in the world of the microbes, where they meet the Roid (Bacteroides), Longo biffi (Bifidobacterium longum), E. …

Hydrophobic Sand Is A Viable Method Of Urine Collection From The Rat For Extracellular Vesicle Biomarker Analysis, Jessica F. Hoffman, Ivan J. Vechetti Jr, Alexander P. Alimov, John F. Kalinich, John J. Mccarthy, Charlotte A. Peterson

Department of Nutrition and Health Sciences: Faculty Publications

Previously we have shown in rats a new method of urine collection, hydrophobic sand, to be an acceptable alternate in place of the traditional method using metabolic cages. Hydrophobic sand is non-toxic, induces similar or lower levels of stress in the rat, and does not contaminate clinical urine markers nor metal concentrations in collected samples (Hoffman et al., 2017 and 2018). Urine is often used in humans and many animal models as a readily-attainable biosample which contains proteins and microRNAs (miRNAs) within extracellular vesicles (EVs) that can be isolated to indicate changes in health. In order to ensure hydrophobic sand …

Full Stem Ahead, Shawn Knowlton, Megan Elbel

Honors Program: Expanded Learning Clubs

After school club that introduces students to STEM and builds problem-solving skills through hands-on, interactive activities.

Brangus Cows Have Ovarian Reserve Parameters More Like Brahman Than Angus Cows, Robert A. Cushman, Émerson M. Soares, Hannah K. Yake, Allyson L. Patterson, Shelby L. Rosasco, Joslyn K. Beard, Emmalee J. Northrop, Jerica J.J. Rich, Jeremy R. Miles, Chadwick C. Chase Jr., Michael G. Gonda, George A. Perry, Anthony K. Mcneel, Adam F. Summers

Roman L. Hruska U.S. Meat Animal Research Center: Reports

Bos indicus females have more surface antral follicles than Bos taurus females; however, histological studies demonstrated no difference in total number of primordial follicles between these two biological types of cattle. Primordial follicle density in the ovary was less in Nelore ovaries compared to Angus ovaries, but no studies have examined the primordial follicle density in Bos indicus cross-bred females. It, therefore, was hypothesized that primordial follicle density in the ovary would decrease as percentage Bos indicus increased. Ovaries were collected from cross-bred Angus (n=32, no Bos indicus influence), Brangus (n=15), or Brahman (n=9) …

Stem Opportunities - High School 2019, Huey-Xian Kelly Wong, Madeleine Rauhauser, Annie Morgan Nelson

Honors Program: Expanded Learning Clubs

This publication details the lesson plan for the “Opportunities in STEM” club for the summer of 2019. This club began out of a desire to educate high school students about the opportunities and careers available in the science, technology, engineering, and mathematics fields. Often, students feel that the prospect of entering a STEM field is daunting and out of their grasp. What it means to be a scientist is often unclear, and students never consider opportunities out of the fear of the unknown, particularly when science is associated with complexity and difficulty. This lesson plan integrates experiments with a wealth …

Comparison Of The Efficacy Of Four Drug Combinations For Immobilization Of Wild Pigs, Christine K. Ellis, Morgan E. Wehtje, Lisa L. Wolfe, Peregrine L. Wolff, Clayton D. Hilton, Mark C. Fisher, Shari Green, Michael P. Glow, Joeseph M. Halseth, Michael J. Lavelle, Nathan P. Snow, Eric H. Vannatta, Jack C. Rhyan, Kurt C. Vercauteren, William R. Lance, Pauline Nol

United States Department of Agriculture Wildlife Services: Staff Publications

Field immobilization of native or invasive wild pigs (Sus scrofa) is challenging. Drug combinations commonly used often result in unsatisfactory immobilization, poor recovery, and adverse side effects, leading to unsafe handling conditions for both animals and humans. We compared four chemical immobilization combinations, medetomidine–midazolam–butorphanol (MMB), butorphanol–azaperone–medetomidine (BAM™), nalbuphine–medetomidine–azaperone (NalMed-A), and tiletamine– zolazepam–xylazine (TZX), to determine which drug combinations might provide better chemical immobilization of wild pigs. We achieved adequate immobilization with no post-recovery morbidity withMMB. Adequate immobilization was achieved with BAM™; however, we observed post-recovery morbidity. Both MMB and BAM™ produced more optimal results relative to body temperature, recovery, and …

Systematic Optimization For Production Of The Anti-Mrsa Antibiotics Wap-8294a In An Engineered Strain Of Lysobacter Enzymogenes, Xusheng Chen, Shanren Li, Lingjun Yu, Amanda Miller, Liangcheng Du

Department of Chemistry: Faculty Publications

WAP-8294A is a group of cyclic lipodepsipeptides and considered as the first-in-class new chemical entity with potent activity against methicillin-resistant Staphylococcus aureus. One of the roadblocks in developing the WAP-8294A antibiotics is the very low yield in Lysobacter. Here, we carried out a systematic investigation of the nutritional and environmental conditions in an engineered L. enzymogenes strain for the optimal production of WAP-8294A. We developed an activity-based simple method for quick screening of various factors, which enabled us to optimize the culture conditions. With the method, we were able to improve the WAP-8294A yield by 10-fold in small-scale cultures and …

An Attenuated Zika Virus Encoding Non-Glycosylated Envelope (E) And Non-Structural Protein 1 (Ns1) Confers Complete Protection Against Lethal Challenge In A Mouse Model, Arun S. Annamalai, Aryamav Pattnaik, Bikash R. Sahoo, Zack P. Guinn, Brianna L. Bullard, Eric A. Weaver, David J. Steffen, Sathish Kumar Natarajan, Thomas M. Petro, Asit K. Pattnaik

School of Veterinary and Biomedical Sciences: Faculty Publications

Zika virus (ZIKV), a mosquito-transmitted flavivirus, emerged in the last decade causing serious human diseases, including congenital microcephaly in newborns and Guillain-Barré syndrome in adults. Although many vaccine platforms are at various stages of development, no licensed vaccines are currently available. Previously, we described a mutant MR766 ZIKV (m2MR) bearing an E protein mutation (N154A) that prevented its glycosylation, resulting in attenuation and defective neuroinvasion. To further attenuate m2MR for its potential use as a live viral vaccine, we incorporated additional mutations into m2MR by substituting the asparagine residues in the glycosylation sites (N130 and N207) of NS1 with alanine …

Vitamin D Supplementation And Prevention Of Type 2 Diabetes, Anastassios G. Pittas, Bess Dawson‑Hughes, Patricia Sheehan, James H. Ware, William C. Knowler, Vanita R. Aroda, Irwin Brodsky, Lisa Ceglia, Chhavi Chadha, Ranee Chatterjee, Cyrus Desouza, Rowena Dolor, John Foreyt, Paul Fuss, Adline Ghazi, Daniel S. Hsia, Karen C. Johnson, Sangeeta R. Kashyap, Sun Kim, Erin S. Leblanc, Michael R. Lewis, Emilia Liao, Anne Peters, Lawrence S. Phillips, Richard Pratley, Philip Raskin, Neda Rasouli, David Robbins, Clifford Rosen, Ellen M. Vickery, Myrlene Staten, Jason Nelson

United States Department of Veterans Affairs: Publications

BACKGROUND

Observational studies support an association between a low blood 25-hydroxyvitamin D level and the risk of type 2 diabetes. However, whether vitamin D supplementation lowers the risk of diabetes is unknown.

METHODS

We randomly assigned adults who met at least two of three glycemic criteria for prediabetes (fasting plasma glucose level, 100 to 125 mg per deciliter; plasma glucose level 2 hours after a 75-g oral glucose load, 140 to 199 mg per deciliter; and glycated hemoglobin level, 5.7 to 6.4%) and no diagnostic criteria for diabetes to receive 4000 IU per day of vitamin D3 or placebo, regardless …

Microsatellite Markers In Spanish Lime (Melicoccus Bijugatus Jacq., Sapindaceae), A Neglected Neotropical Fruit Crop, Jaime Martinez-Castillo, Renee S. Arias, Ruben H. Andueza-Noh, Matilde M. Ortiz-Garcia, Brian M. Irish, Brian E. Scheffler

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Spanish lime (Melicoccus bijugatus Jacq.) is aNeotropical fruit tree cultivated, mainly, in orchards for self-consumption or local sale. The genus Melicoccus includes other nine species with edible fruits, some of these species are at risk of extinction. Like for the vast majority of tropical fruit trees, there is no information on the genetic diversity of Spanish lime and its related species, and this is mostly due to the lack of molecular markers. The objectives of this study were to present the first microsatellite markers developed for Spanish lime, testing its usefulness on a sample of cultivated accessions, as well as …

Water Productivity In Meat And Milk Production In The Us From 1960 To 2016, Mesfin Mekonnen, C.M.U. Neale, Chittaranjan Ray, Galen E. Erickson, Arjen Y. Hoekstra

Daugherty Water for Food Global Institute: Faculty Publications

Global demand for livestock products is rising, resulting in a growing demand for feed and potentially burdening freshwater resources to produce this feed. To offset this increased pressure on water resources, the environmental performance of livestock sector should continue to improve. Over the last few decades, product output per animal and feedstuff yields in the US have improved, but before now it was unclear to what extent these improvements influenced the water productivity (WP) of the livestock products. In this research, we estimate changes in WP of animal products from 1960 to 2016. We consider feed conversion ratios (dry matter …

Bacteria-Mediated Modification Of Insecticide Toxicity In The Yellow Fever Mosquito, Aedes Aegypti, Sara S. Scates, Scott T. O'Neal, Troy D. Anderson

Department of Entomology: Faculty Publications

The incidence of mosquito-borne disease poses a significant threat to human and animal health throughout the world, with effective chemical control interventions limited by widespread insecticide resistance. Recent evidence suggests that gut bacteria of mosquitoes, known to be essential in nutritional homeostasis and pathogen defense, may also play a significant role in facilitating insecticide resistance. This study investigated the extent to which bacteria contribute to the general esterase and cytochrome P450 monooxygenase (P450)-mediated detoxification of the insecticides propoxur and naled, as well as the insecticidal activity of these chemistries to the yellow fever mosquito, Aedes aegypti. Experiments conducted using …

Nebline, August 2019

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

Kids Eat Right Month

Food Safety Tips for Electric Multi-Cookers

Recipe of the Month

Soybean Management Field Days

Landlord–Tenant Cash Rent Workshop, Aug. 21

Fall is Best for Control of Tough Weeds

Free Water Screening for Nitrates

Include Pets in Your Emergency Plan

Going Batty: Bugs on Bats

Make Your Yard a Greener Place

Garden Guide: Things to Do This Month

Tips for a Fun and Educational Day at the Fair

Upcoming Early Childhood Trainings

Heart of 4-H Volunteer Award: Susan Holland

4-H’ers Test Family and Consumer Science and Entrepreneurship Skills at Life Challenge

4-H’ers Test Their Knowledge of Animals …

Exploring Rural And Urban Go Nap Sacc Trained Child Care Providers Perceptions And Needs Regarding The Promotion Of Physical Activity And Healthy Eating, Kailey Snyder, Zainab Rida, Emily Hulse, Dipti Dev, Danae Dinkel

Department of Child, Youth, and Family Studies: Faculty Publications

Introduction: Early childhood is an optimal time to support the development of physical activity and healthy eating behaviors. As over half of children are cared for in family child care homes and child care centers it is crucial to ensure these behaviors are being supported in the childcare setting. One such process that supports provider’s education and implementation of healthy behaviors in the childcare setting is the Nutrition and Physical Activity Assessment in Child Care (Go NAP SACC). However, after participation in Go NAP SACC, little is known regarding how to further support providers in their promotion of healthy behaviors. …

An In Vitro Enrichment Strategy For Formulating Synergistic Synbiotics, Car Reen Kok, David Fabian Gomez Quintero, Clement Niyirora, Devin Rose, Amanda Li, Robert Hutkins

Food for Health: Publications

ABSTRACT Research on the role of diet on gut and systemic health has led to considerable interest toward identifying novel therapeutic modulators of the gut microbiome, including the use of prebiotics and probiotics. However, various host responses have often been reported among many clinical trials. This is in part due to competitive exclusion as a result of the absence of ecological niches as well as host mediated constraints via colonization resistance. In this research, we developed a novel in vitro enrichment (IVE) method for isolating autochthonous strains that can function as synergistic synbiotics and overcome these constraints. The method relied …

Post–Modern Epidemiology: When Methods Meet Matter, George Davey Smith

United States Public Health Resources

In the last third of the 20th century, etiological epidemiology within academia in high-income countries shifted its primary concern from attempting to tackle the apparent epidemic of noncommunicable diseases to an increasing focus on developing statistical and causal inference methodologies. This move was mutually constitutive with the failure of applied epidemiology to make major progress, with many of the advances in understanding the causes of noncommunicable diseases coming from outside the discipline, while ironically revealing the infectious origins of several major conditions. Conversely, there were many examples of epidemiologic studies promoting ineffective interventions and little evident attempt to account for …

Taste Manipulation And Swallowing Mechanics In Trauma-Related Sensory-Based Dysphagia, Angela M. Dietsch, H. Duncan Dorris, William Pearson, Katie E. Dietrich-Burns, Nancy Pearl Solomon

Department of Special Education and Communication Disorders: Faculty Publications

Purpose: This study explored the effects of highconcentration taste manipulation trials on swallow function in persons with sensory-based dysphagia.

Method: Dysphagia researchers partnered with clinical providers to prospectively identify traumatically injured U.S. military service members (N = 18) with sensorybased dysphagia as evidenced by delayed initiation and/or decreased awareness of residue/penetration/ aspiration. Under videofluoroscopy, participants swallowed trials of 3 custom-mixed taste stimuli: unflavored (40% weight/volume [wt/vol] barium sulfate in distilled water), sour (2.7%wt/vol citric acid in 40% wt/vol barium suspension), and sweet–sour (1.11% wt/vol citric acid plus 8% wt/vol sucrose in 40% wt/vol barium suspension). Trials were analyzed …

Oxygenation And Activation Of The Vastus Lateralis During Dynamic Constant External Resistance Leg Extension Muscle Actions In Older Women With And Without Sarcopenia, Brianna D. Mckay

College of Education and Human Sciences: Dissertations, Theses, and Student Research

The purpose of the present study was to compare muscle strength, size, activation, and oxygenation between older women with and without sarcopenia during dynamic fatiguing leg extension bouts with high (5-repetition maximum[5-RM]) and low (30% of estimated 1-RM[30%1-RM]) loads. Eleven women (n = 6 non-sarcopenic [mean ± SE; age = 75.8 ± 2.6y] and n = 5 sarcopenic [age = 74.5 ± 3.1y]) were screened for eligibility and sarcopenic status. Descriptive assessments including demographics (age, height, and weight), body composition by dual-energy x-ray absorptiometry (fat mass[FM], fat-free mass[FFM] and percent body fat[BF%]), muscle size by ultrasonography (leg extensor muscle cross-sectional …

Promoting Bee Communities Through Habitat Enhancements On Public And Private Lands In Nebraska, Kayla A. Mollet

Department of Entomology: Dissertations, Theses, and Student Research

Wild and managed bees are the most effective pollinators, accounting for about 80% of the pollination of flowering plants and 75% of fruits, nuts, and vegetables in the United States (USDA, 2019; USFWS, 2019). An estimated 4,000 species of bees reside in North America, the majority of which are wild and unmanaged. Wild bee communities are critical for maintaining healthy ecosystems, as they sustain native flora that provides soil stability and habitat for other wildlife. In a changing landscape, floral enhancements on privately and publicly-owned lands may have great impact for improving habitat for pollinators across the United States. Planting …

3-Hydroxy Fatty Acids Induce Retinal Pigment Epithelial Cell Lipoapoptosis, Mona Hadidi

Department of Nutrition and Health Sciences: Dissertations, Theses, and Student Research

Mitochondrial fatty acid oxidation (FAO) plays an important role in maintaining metabolic homeostasis, especially during catabolic stress. Genetic mutation in gene that encodes for mitochondrial FAO enzymes could result in metabolic stress especially during fasting or hypoglycemic condition. Mutation in the active site residue (1528G>C) of long chain 3-hydroxy acyl CoA dehydrogenase (LCHAD) results in the loss of LCHAD enzyme activity. Children’s with LCHAD mutation were known to accumulate 3-hydroxy fatty acids such as 3-hydroxy palmitic acid and 3-hydroxy myristic acids, long chain free fatty acids, acyl carnitine and 3-hydroxy dicarboxylic acid due to their defective enzyme activity. These …

Inhibitory Effects Of Shiitake-Derived Exosome-Like Nanoparticles On Nlrp3 Inflammasome Activation, Yizhu Lu

Department of Nutrition and Health Sciences: Dissertations, Theses, and Student Research

The NLRP3 inflammasome is a critical mediator of inflammation and consists of the sensor NOD-like receptor family, pyrin domain containing 3 (NLRP3), the adaptor apoptotic speck protein containing a caspase recruitment domain (ASC), and the effector caspase-1. Dysregulated or excessive activation of the NLRP3 inflammasome contributes to pathogenesis of diverse inflammatory diseases such as Type 2 diabetes and atherosclerosis. Therefore, the NLRP3 inflammasome is a promising therapeutic target for treating these diseases. Extracellular vesicles (EVs) are membrane-enclosed tiny particles released by almost any type of cells and they are involved in the intercellular communication by transferring their cargos including RNAs, …

Transport And Distribution Of Bovine Milk Exosomes And Mir-34a Cargo In Murine Cerebral Cortex Endothelial Bend.3 Cells And Bv2 Microglia, Pearl Ebea

Department of Nutrition and Health Sciences: Dissertations, Theses, and Student Research

The blood-brain barrier (BBB) poses an obstacle in the delivery of drugs to the brain. Bovine milk exosomes (BME) are explored for delivering antisense oligonucleotides to tumors, because BME are bioavailable and protect RNA cargos against degradation in the gastrointestinal tract. This study had the following objectives: 1) assess the transport kinetics of BME and their RNA cargos and secretion of RNA across the apical membrane in murine cerebral cortex endothelial bEnd.3 cells and 2) determine whether murine brain BV2 microglia have the potential to accumulate and, therefore, eliminate BME that crossed the BBB. The uptake of BME labeled with …

Which Credence Production Attributes Do Consumers Prefer? The Case Of Milk, Emmanouil Petrakis

Department of Agricultural Economics: Dissertations, Theses, and Student Research

The interest of consumers for non-traditional food production attributes like antibiotics free and animal welfare have made consumer preferences more complex. These new attributes, usually non-identifiable by inspection or experience of the product are called credence attributes. The objectives of this study are to analyze consumer preferences for milk production attributes by eliciting the relative importance of these attributes for consumers, and to identify possible sources of heterogeneity related to consumer characteristics and actual purchase data. The research method used to elicit consumer preferences is Paired Comparison Method. Consumers from California and Texas were administered the online survey questionnaire in …

How Does Identifying As Gluten-Free Impact Information Choice Regarding The Gluten-Free Diet?, Pratiksha Baishya

Department of Agricultural Economics: Dissertations, Theses, and Student Research

The market for gluten-free products is a multi-billion-dollar industry in the United States and has seen tremendous growth in the recent years. The retail sales of gluten-free foods in the United States almost tripled between 2011 and 2015, although rates of diagnosed gluten-related health problems have not risen. In addition to people who suffer from Celiac Disease, Wheat Allergy and Non-Celiac Gluten Sensitivity, there is a category or people known as PWAG (people who avoid gluten) who seem to have significantly contributed to this boom in the market for gluten-free foods. With more people choosing to adopt the gluten-free diet, …

Dual Biological Control: Characterization Of Fungi And Bacteria To Control Granary Weevil And Fungal Pathogens Of Stored Grain, Gülçin Ercan

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Cereals are main food sources for humans and animals. However, during storage, cereal grains can be infested by insects and fungi. One of the most important insect storage pests is Sitophilus granarius (L., Coleoptera: Curculionidae). Adults and larvae can cause serious grain losses. In addition to insect pests, fungal pathogens may also invade the grain and cause economic loss, including contamination with mycotoxins, which threaten mammal health by causing serious disease. The most common mycotoxigenic grain fungi are species that belong to the genera Fusarium, Aspergillus and Penicillium. Currently, the most commonly used management strategies for insect and …