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The Specific Activities Of Shiga-Like Toxin Type Ii (Slt-Ii) And Slt-Ii-Related Toxins Of Enterohemorrhagic Escherichia Coli Differ When Measured By Vero Cell Cytotoxicity But Not By Mouse Lethality, Susanne W. Lindgren, James E. Samuel, Clare K. Schmitt, Alison D. O'Brien

Uniformed Services University of the Health Sciences

Characteristically, enterohemorrhagic Escherichia coli (EHEC) strains produce Shiga-like toxin type I (SLT-I), SLT-II, or both of these immunologically distinct cytotoxins. No antigenic or receptor-binding variants of SLT-I have been identified, but a number of SLT-II-related toxins have been described. Because EHEC 091:H21 strain B2F1, which produces two SLT-II-related toxins, is exquisitely virulent in an orally infected, streptomycin-treated mouse model (oral 50% lethal dose [LD₅₀], <10 >organisms), we asked whether the pathogenicity of strain B2F1 was a consequence of SLT-II-related toxin production. For this purpose, we compared the lethality of orally administered E. coli DH5α(Str^r) strains that produced …

Haemophilus Parainfluenzae Endocarditis: Application Of A Molecular Approach For Identification Of Pathogenic Bacterial Species, Kamal A. Hamed, Philip R. Dormitzer, Catherine K. Su, David A. Relman

United States Department of Veterans Affairs: Publications

Haemophilus parainfluenzae is both a human oropharyngeal commensal bacterium and a cause of serious invasive disease. The fastidious growth characteristics of this organism and the poor specificity of traditional methods for species identification are likely to have led to inaccuracies in the diagnosis of infections caused by H. parainfluenzae and related organisms. We report a case of H. parainfluenzae endocarditis in which confusion related to microbial identification was resolved by the analysis of 16S ribosomal RNA sequences. Rapid identification was facilitated by amplification of 16S ribosomal DNA directly from cultured cells with use of the polymerase chain reaction …

Promoting Collaborative Learning In The Early Childhood Classroom: Reggio Emilia, Italy, And Amherst, Massachusetts., Carolyn P. Edwards, Lella Gandini, John Nimmo

Department of Psychology: Faculty Publications

This chapter describes a study that was conducted in Reggio Emilia (Emilia Romagna, northern Italy), Pistoia (Tuscany, Italy) and Amherst (Massachusetts, USA) as part of a three study project investigating how teachers promote collaboration and community in their classrooms. The methodology involved a combination of teacher interviews with an adaptation of the “multivocal video-ethnography developed by Tobin, Wu, and Davidson (1989) for Preschool in Three Cultures. This paper describes the findings from the Collaboration Interviews with teachers in Reggio Emilia and Amherst, showing intersections and differences in their concepts of young children’s capacities for cooperation and teachers’ roles in …

Colony Isolation And Secondary Culture Of Fetal Porcine Hepatocytes On Sto Feeder Cells, Neil C. Talbot, Vernon G. Pursel, Caird E. Rexroad Jr., Thomas J. Caperna, Anne M. Powell, Roger T. Stone

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

The secondary culture of non-transformed parenchymal hepatocytes has not been possible. STO feeder cell-dependent secondary cultures of fetal pig hepatocytes were established by colony isolation from primary cultures of 26-d fetal livers. The liver cells had the typical polygonal morphology of parenchymal hepatocytes. They also spontaneously differentiated to form small biliary canaliculi between individual cells or progressed further to large multicellular duct-like structures or cells undergoing gross lipid accumulation and secretion. The secondary hepatocyte cultures expressed alpha-fetoprotein (AFP), albumin, and β-fibrinogen mRNA, and conditioned medium from the cells contained elevated levels of transferrin and albumin. STO feeder cell co-culture may …

Effects Of Offshore Oil And Gas Development: A Quarterly Current Awareness Bibliography, January 1994, Sue Ann Gardner

University of Nebraska-Lincoln Libraries: Faculty Publications

The January 1994 issue of Effects of Offshore Oil and Gas Development: A Quarterly Current Awareness Bibliography.

Homework: A Natural Means Of Home-School Collaboration, Daniel E. Olympia, Susan M. Sheridan, William Jenson

Department of Educational Psychology: Faculty Publications

The purpose of this article is to place homework in the framework of an emerging interest in the development of effective home-school partnerships. Specifically, review of the homework literature is provided with attention to parent-, teacher-, and child-mediated programs. Several homework programs that have received empirical support are described. Finally, the role of the school psychologist in supporting and coordinating complementary home-school homework programs is explored.

Exopeptidase Catalyzed Site-Specific Bonding Of Supports, Labels And Bioactive Agents To Proteins, Fred W. Wagner, Thomas R. Coolidge, Dwane E. Wylie, Sheldon M. Schuster, William Lewis, Jay Stout

Department of Biochemistry: Faculty Publications

The invention provides a means for attaching a label, support or bioactive agent to a protein with an exopeptidase at a site that is remote from the active site of the protein. More specifically the invention is directed to a method for the attachment of an amino acid, amine and alcohol nucleophile to the carboxyl terminus of a protein. In one embodiment, a labeled nucleophile is attached to a protein such as an antibody. In other embodiments, the invention is directed to a method for the attachment of a protein to an immobilization support and to a method for the …

Nicotinic Acid Hydroxylase From Clostridium Barkeri: Electron Paramagnetic Resonance Studies Show That Selenium Is Coordinated With Molybdenum In The Catalytically Active Selenium-Dependent Enzymenicotinic Acid Hydroxylase From Clostridium Barkeri: Electron Paramagnetic Resonance Studies Show That Selenium Is Coordinated With Molybdenum In The Catalytically Active Selenium-Dependent Enzyme, Vadim Gladyshev, Sergei V. Khangulov, Thressa C. Stadtman

Vadim Gladyshev Publications

Nicotinic acid hydroxylase from Clostidum barkeri contains selenium in an unidentified form that is dissociated as a low molecular weight compound upon denaturation of the enzyme. Other cofactors of this enzyme are molybdopterin, FAD, and iron-sulfur clusters. In the current study, we show that the enzyme, as isolated, exhibits a stable Mo(V) electron paramagnetic resonance (EPR) sal ("resting" signal) and that this signal is correlated with the selenium content and nicotinate hydroxylase activity of the enzyme. Substitution of77Se for normal selenium isotope abundance results in splitting of the Mo(V) EPR signal of the native protein without affecting the iron signals …

Otitis Media, Mastoiditis, And Infracranial Lesions In Two Plains Indian Children, Robert W. Mann, Douglas W. Owsley, Karl J. Reinhard

Karl Reinhard Publications

One disease condition that is common to nearly all children is otitis media, also known as middle ear disease. Although most people speak of it as a middle ear "infection," other causes such as neoplasm (tumor) can result in manifestations and symptoms mimicking an infectious process. This paper presents findings related to osseous lesions in two Indian children, approximately three to five years of age, who died with otitis media and infracranial lesions. Through macroscopic and radiographic analysis, it is possible to show that one child probably suffered from tuberculosis and the other from histiocytosis X. From an epidemiological and …

Initial Reference Models In Local Earthquake Tomography, E. Kissling, W. L. Ellsworth, D. Eberhart-Phillips, U. Kradolfer

United States Geological Survey: Staff Publications

The inverse problem of three-dimensional (3-D) local earthquake tomography is formulated as a linear approximation to a nonlinear function. Thus the solutions obtained and the reliability estimates depend on the initial reference model. Inappropriate models may result in artifacts of significant amplitude. Here, we advocate the application of the same inversion formalism to determine hypocenters and one-dimensiona(1-D) velocity model parameters, including station corrections, as the first step in the 3-D modeling process. We call the resulting velocity model the minimum 1-D model.  For test purposes,  synthetic data set based on the velocity structure of the San Andreas fault zone in …

Effects Of Lithology And Depth On The Permeability Of Core Samples From The Kola And Ktb Drill Holes, C. Morrow, D. Lockner, S. Hickman, M. Rusanov, T. Röckel

United States Geological Survey: Staff Publications

Permeability measurements were conducted on intact core samples from
the Kola drill hole in Russia and the KTB drill hole in Germany. Samples included granodiorite gneisses, basalts and amphibolites from depths up to 11 km. The tests were intended to determine the pressure sensitivity of permeability and to compare the effects of stress relief and thermal microcracking on the matrix permeability of different rock types and similar samples from different depths. The pore pressure P_p was fixed at the estimated in situ pressure assuming a normal hydrostatic gradient;
the confining pressure P_c was varied to produce effective pressures(P …

Two-Dimensional Transport Model For Variably Saturated Porous Meda With Major Ion Chemistry, Jiří Šimůnek, Donald L. Suarez

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

We present the development and demonstrate the use of the two-dimensional finite element code UNSATCHEM-2D for modeling major ion equilibrium and kinetic nonequilibrium chemistry in variably saturated porous media.  The model is intended for prediction of major ion chemistry and water and solute fluxes for soils under transient conditions.  Since the solution chemistry in the unsaturated zone is significantly influenced by variations in water content, temperature, and CO₂ concentrations in the soil gas, all these variables are also calculated by the model.  The major variables of the chemical system are Ca, Mg, Na, K, SO₄, Cl, NO …

A Continuous Culture Of Pluripotent Fetal Hepatocytes Derived From The 8-Day Epiblast Of The Pig, Neil C. Talbot, Caird E. Rexroad Jr., Anne M. Powell, Vernon G. Pursel, Thomas J. Caperna, Sherry L. Ogg, Neil D. Nel

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Continuous cultures of pluripotent prenchymal hepatocytes were derived from the epiblasts of 8-day-old pig blastocytes. The cells were polygonal and had phase-contrast dark, granular cytoplasm with prominent nuclei and nucleoli. These feeder-dependent cell cultures differentiated into large, multicellular, secretory, duct-like structures or formed small canaliculi between individual cells. Alternatively, the cells accumulated droplets that stained intensely with Oil Red O, a lipid-specific stain. Alpha-fetoprotein (AFP), albumin, and β-fibrinogen mRNAs were expressed as the cells differentiated in culture. Serum-free medium that was conditioned by the cells contained transferrin, AFP, and albumin. The growth and viability of the cells were inhibited by …

Rejex-It'" Ag-36 The Gentle Way To Repel Geese From Golf Courses And Other Turf Areas, Peter F. Vogt

Great Plains Wildlife Damage Control Workshop Proceedings

Birds have always been a problem in agriculture, specially for berries and fruits. Farmers and home owners have been fighting these problems with limited success for centuries. However, the problems with birds on turf are relatively new and are not even known by many people. The "Complete Guide to Pest Control" (Ware 1988) does not even mention geese as a pest, or golf courses as an area of bird problems.

Prospects For The Future In Animal Damage Control, Donald Hawthorne

Great Plains Wildlife Damage Control Workshop Proceedings

I am pleased to be here to address a subject that is very special to many of us, one to which I have devoted my entire career. Animal damage control is a controversial component of wildlife management. In the late 1960's and early 70's, many wildlife practitioners tried to divorce themselves from animal damage control activities. In recent years, however, management of wildlife damage is viewed by many as a vital and integral part of wildlife management, whether to protect human interests and safety or to save an endangered species.

Future Extension Programs On Wildlife Damage Management, James E. Miller

Great Plains Wildlife Damage Control Workshop Proceedings

Extension educational programs, in cooperation with other federal and state organizations, significantly contribute to the present status of wildlife damage management as understood by natural resources professionals and the interested public. Although Extension Service, USDA provides national leadership and coordination of programs, most of the effective programs originate at the state and local level Cooperative Extension Service, regardless of the subject matter. This is particularly true in the area of wildlife damage management. For example, a recent compilation of Extension publications and videotapes on wildlife damage management identifies well over 100 titles available throughout the CES System (Ruff, Craven and …

Developing Risk Communication Skills: More Than Damage Management, Deanne Wright

Great Plains Wildlife Damage Control Workshop Proceedings

Risk communication provides a methodology that enables an organization to respond effectively to issues or situations of a controversial nature. It is one important aspect of risk management.

Urban Nuisance Wildlife Control In Kentucky, Thomas G. Barnes

Great Plains Wildlife Damage Control Workshop Proceedings

A variety of factors including increased urbanization, decreased funding for governmental animal damage programs, and increases in some urban wildlife populations have resulted in a greater demand for urban nuisance wildlife control. Historically, this demand was met by Cooperative Extension Service (San Julian 1987), state fish and wildlife agency, or USDA-APHIS-ADC employees (Bollengier 1987). These agencies provided educational materials, consultations, and/or physically removed animals. Recently, there is an increased demand for physical animal removal evidenced by increasing numbers of private pest control operators (PCO) specializing as urban nuisance wildlife control operators (NWCO).

How To Involve Local Communities In Wildlife Damage Control Decision-Malting, Scott R. Craven

Great Plains Wildlife Damage Control Workshop Proceedings

In most cases the impetus for wildlife damage control (ADC) comes from the individual or group victimized by the problem, i.e., the farmers losing crops to migrating waterfowl or the homeowners frustrated with the loss of valuable ornamental plants to marauding deer. These "victims" or "stakeholders" are clearly best positioned to describe the nature and extent of the problem, how they feel about it, and to comment on what action could/should be taken to reduce or end the problem.

Acuta Enews December 1993, Vol. 22, No. 12

ACUTA Newsletters

In This Issue

Board Report

President's message

Telecom talks $$$ at UNM

US West to test high speed transmission

Big MAN on campus

Student toll fraud

Nebline, December 1993

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

Horticulture

Rural $ense

Human Resources

4-H & Youth

Environmental Focus

Community & Leadership Development

Extension Calendar

Expanded Food and Nutrition Education Program (EFNEP)

Nebraska Association for Family and Community Education News

and other extension news and events

Wildlife Exposure Factors Handbook, Volume I Of Ii, United States Environmental Protection Agency

United States Environmental Protection Agency: Publications

The Exposure Assessment Group of the Office of Health and Environmental Assessment (OHEA) has prepared the Wildlife Exposure Handbook in support of the Office of Solid Waste and Emergency Response and the Office of Water. The Handbook provides information on various factors used to assess exposure to wildlife. The goals of the project are (1) to promote the application of risk assessment methods to wildlife species, (2) to foster a consistent approach to wildlife exposure and risk assessments, and (3) to increase the accessibility of the literature applicable to these assessments.
The bulk of the document summarizes literature values for …

Water Current, Volume 25, No. 4, December 1993

Water Current Newsletter

Grew: Politics Change Rationale for Research
From the Director
Husker Harvest Winners
Needs of Research, Editor Differ
Conjunctive Use Conference Focus
1994 Seminars Look at State's Water History
Variety of Voices Heard at Symposium
Research Brief: Comparitve Toxicity of Pyrethroid Insecticides to Terrestrial and Aquatic Insects
Surface Water Management Interest of Faculty Member
Diretory Lists Wetlands Faculty
Scientists Examine Irrigation with Swine Waste Water
Platte River Atlas Examines Multi-Faceted Big Bend Area
Looking for Resources Information?

Pod Network News, November 1993

Professional and Organizational Development Network in Higher Education: POD Network News

POD Conference in Rochester

Report on CORE Committee Decisions

Winners of the Bright Idea Awards

1994 POD Conference in Portland

Thanks to Editors of To Improve the Academy

POD Electronic Network

CORE Meeting at AAHE

Newsletter Enclosures about Upcoming Events

Announcements: Conferences, Journals, Employment Opportunity

From the President

POD CORE Committee, 1994-1997 Self-Nomination Form

Call for Papers for the 1994 Edition of To Improve The Academy

POD Network 1994-95 POD Grant Program

Membership Form

Potential Use Of Contraception For Managing Wildlife Pests In Australia, Mary Bomford, Peter O'Brien

Contraception in Wildlife Management

There is an increasing level of interest in contraception to manage wildlife pests in Australia, due mainly to concerns over high recurrent costs, animal welfare, and the failure of current control techniques to prevent damage in some instances. We have developed criteria that need to be met for contraception to be successful for pest control:
• Technology exists to reduce fertility
• An effective delivery mechanism to treat wild animals exists.
• The end result of reduced animal damage is achieved.
• Effects are humane and nontoxic.
• Product is target specific, cost effective, and environmentally acceptable.

Human Dimensions Of Contraception In Wildlife Management, Paul D. Curtis, Daniel J. Decker, Rebecca J. Stout, Milo E. Richmond, Cynthia A. Loker

Contraception in Wildlife Management

Wildlife damage management was so much simpler in the good old days. If deer (Odocoileus virginianus), beaver (Castor canadensis), or other animals were a problem in a particular situation, people simply had them shot, trapped, or poisoned. Not many years ago, most people would go along with this approach, and those who didn't like it were marginalized as the "radical fringe." Not so today. Greater and more diverse segments of the public want a say in what professionals decide to do with their wildlife. The public wants to participate in setting objectives for management and in …

The Applicability And Biopolitics Of Contraceptive Techniques For Deer Management, Robert J. Warren, Lisa M. White

Eastern Wildlife Damage Control Conference: 6th (1993)

While regulated public hunting or controlled lethal reduction programs are effective in controlling white-tailed deer (Odocoileus virginianus), populations in most areas, increasingly there are settings (e.g., urban and suburban environments) where such programs are either unsafe or publicly unacceptable. Past research with contraceptive techniques in deer have shown these techniques to be either ineffective or infeasible for managerial implementation. Current research with immunocontraceptives show promise as being both effective and feasible for field application. Immunocontraceptive vaccines can be delivered remotely and are highly effective in causing infertility in most treated does. Much more research is needed before these techniques can …

A Cooperative Approach To Resolving A Marmot Damage Problem In An Urban Recreational Site, Layne R. Bangerter

Eastern Wildlife Damage Control Conference: 6th (1993)

The United States Army Corps of Engineers (COE) requested that USDA, APHIS, Animal Damage Control (ADC) investigate methods of yellowbelly marmot (Marmota flaviventris) removal along a parkway and levee system in Lewiston, Idaho. COE biologists determined that burrowing marmots had penetrated and were compromising the integrity of the levee core. In addition to protecting downtown Lewiston from flooding, the levee is used as a popular bicycle and foot path. The Humane Society of the United States (HSUS), and other groups and individuals became involved at meetings held to discuss potential methods of resolving the problem. ADC in cooperation with HSUS …

Strategies To Address Human Conflicts With Raccoons And Black Bears In New Hampshire, Marsha E. Barden, Dennis Slate, Robert T. Calvert, Paul W. Debow

Eastern Wildlife Damage Control Conference: 6th (1993)

The Cooperative Animal Damage Control program in New Hampshire has received increasing requests for assistance over the past 5 years. This trend is due largely to changing public concern over black bears (Ursus americanus) in residential areas and an epizootic of the Mid-Atlantic strain of rabies in raccoons (Procyon lotor). A growing number of requests is related to improper garbage storage and feeding of songbirds during late spring and summer. Some traditional methods, especially livetrapping and relocation, are not generally appropriate to resolve these conflicts. In this paper we discuss trends in requests for assistance from 1988 to 1992 and …

Can We Landscape To Accommodate Deer? The Tracy Estate Research Garden, Helen H. Heinrich, Susan Predl

Eastern Wildlife Damage Control Conference: 6th (1993)

The Morris County Park Commission, manager of hundreds of acres of open space in this central New Jersey county, realized that the white-tailed deer residing on park land were having a detrimental effect on the vegetation on these properties. A Wildlife Management Advisory Committee to the Commission was formed to measure the dimensions of the problem and to find ways to mitigate the effects of the deer population of deer. One recommendation of the Committee was to determine if a landscape design could be developed to use plantings less attractive to the deer; planted in ways that might discourage heavy …