Open Access. Powered by Scholars. Published by Universities.®
- Discipline
-
- Life Sciences (4020)
- Social and Behavioral Sciences (3514)
- Education (2907)
- Physical Sciences and Mathematics (2443)
- Arts and Humanities (2091)
-
- Engineering (1624)
- Environmental Sciences (1433)
- Higher Education (1275)
- Plant Sciences (1055)
- Medicine and Health Sciences (1027)
- Sociology (843)
- Communication (804)
- Curriculum and Instruction (787)
- Agriculture (786)
- Educational Administration and Supervision (763)
- Art and Design (667)
- Animal Sciences (638)
- Higher Education Administration (608)
- International and Area Studies (608)
- Teacher Education and Professional Development (568)
- Library and Information Science (563)
- Natural Resources and Conservation (521)
- History (505)
- Other Education (504)
- Entomology (495)
- Biochemistry, Biophysics, and Structural Biology (478)
- Political Science (476)
- Law (461)
- Other Plant Sciences (454)
- Natural Resources Management and Policy (437)
- Keyword
-
- Nebraska (154)
- Education (112)
- Multilingual education (88)
- Drought (78)
- United States (74)
-
- Technology (63)
- Gender (60)
- Higher education (59)
- Extension circular (52)
- Pedagogy (52)
- Invasive species (48)
- Agriculture (47)
- Social media (47)
- Teaching (46)
- Management (44)
- Media (43)
- Strategy (41)
- Leadership (38)
- Water (38)
- Art (36)
- Climate change (36)
- Identity (36)
- Trematoda (35)
- Cattle (33)
- Communication (33)
- Corn (33)
- Architecture (32)
- Journalism (32)
- Rhetoric (32)
- Sustainability (31)
- Publication Year
- Publication
-
- United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications (371)
- United States Department of Agriculture Wildlife Services: Staff Publications (344)
- Honors Program: Senior Projects (Public) (327)
- China Beat Blog: Archive 2008-2012 (308)
- Textile Society of America: Symposium Proceedings (294)
-
- Nebraska Law Review (274)
- Department of Biochemistry: Faculty Publications (247)
- Department of Sociology: Faculty Publications (217)
- Court Review: Journal of the American Judges Association (214)
- Department of Teaching, Learning, and Teacher Education: Faculty Publications (210)
- Department of Psychology: Faculty Publications (209)
- ACUTA Newsletters (208)
- Department of Plant Pathology: Faculty Publications (207)
- Department of Agricultural and Biological Systems Engineering: Faculty Publications (182)
- School of Natural Resources: Faculty Publications (181)
- Department of Agronomy and Horticulture: Faculty Publications (180)
- Copyright, Fair Use, Scholarly Communication, etc. (178)
- Dialogue: The Interdisciplinary Journal of Popular Culture and Pedagogy (174)
- School of Biological Sciences: Faculty Publications (172)
- School of Veterinary and Biomedical Sciences: Faculty Publications (168)
- NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County (161)
- Journal of the National Collegiate Honors Council Online Archive (160)
- To Improve the Academy: A Journal of Educational Development (149)
- Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023– (147)
- Department of Mechanical and Materials Engineering: Faculty Publications (145)
- Great Plains Quarterly (through 2013) (145)
- University of Nebraska-Lincoln Extension: Historical Materials (142)
- United States Geological Survey: Staff Publications (139)
- Department of Food Science and Technology: Faculty Publications (136)
- Department of Entomology: Faculty Publications (130)
- Publication Type
- File Type
Articles 14941 - 14970 of 16693
Full-Text Articles in Entire DC Network
Magnetic Properties Of Nanometer-Size Copt Particles, Sy_Hwang Liou, Yi Liu, S.S. Malhotra, M. Yu, David J. Sellmyer
Magnetic Properties Of Nanometer-Size Copt Particles, Sy_Hwang Liou, Yi Liu, S.S. Malhotra, M. Yu, David J. Sellmyer
Sy-Hwang Liou Publications
We have prepared nanometer-size, isolated CoPt particles. The particles in the range of 100–300 nm in diameter were formed by annealing thin films in the temperature range of 550–800 °C. Films with magnetic coercivity as high as 30 kOe were achieved. The results indicate that the high magnetic coercivity was obtained because the crystallite size approaches that of noninteracting single-domain particles.
Magnetic Viscosity And Switching Volumes Of Annealed Fe/Pt Multilayers, C.P. Luo, Z.S. Shan, David J. Sellmyer
Magnetic Viscosity And Switching Volumes Of Annealed Fe/Pt Multilayers, C.P. Luo, Z.S. Shan, David J. Sellmyer
David Sellmyer Publications
Magnetic viscosity and the phenomenon of the sweep-rate dependence of the coercivity are important for the characterization of magnetic recording media. The activation volume of magnetization reversal or switching volume is directly related to medium noise. The magnetic viscosity behavior of annealed Fe/Pt multilayers was studied and the switching volumes were obtained by both measurements of the sweep-rate dependence of coercivity and time dependence of magnetization. It is found that samples with larger coercivities have smaller switching volumes, and an estimate of the magnetic grain diameter is about 9 nm. The coercivity mechanisms are also discussed.
High Coercivity Rare Earth–Cobalt Films, S.S. Malhotra, Yi Liu, Z.S. Shan, Sy_Hwang Liou, D.C. Stafford, David J. Sellmyer
High Coercivity Rare Earth–Cobalt Films, S.S. Malhotra, Yi Liu, Z.S. Shan, Sy_Hwang Liou, D.C. Stafford, David J. Sellmyer
Sy-Hwang Liou Publications
Rare earth–cobalt (RCo, R=Sm, Pr) films with thicknesses from 30 to 700 nm have been prepared with and without a Cr underlayer by dc magnetron sputtering from a R2Co7 composite target. The as-deposited SmCo films with a Cr underlayer (SmCo║Cr) have magnetic coercivities of about 500–2800 Oe and the PrCo║Cr films have coercivities of about 100–300 Oe, but after annealing at 500 °C coercivities as high as 31 kOe for SmCo║Cr films and 10 kOe for PrCo║Cr films were observed. The as-deposited PrCo films are composed mostly of an amorphous phase with about 30 vol % of …
Superparamagnetic Magnetic Force Microscopy Tips, P.F. Hopkins, John Moreland, S.S. Malhotra, Sy_Hwang Liou
Superparamagnetic Magnetic Force Microscopy Tips, P.F. Hopkins, John Moreland, S.S. Malhotra, Sy_Hwang Liou
Sy-Hwang Liou Publications
We report on magnetic force microscopy (MFM) images of a thin-film magnetic recording head taken using batch micromachined silicon tips coated with nanocomposite Fe60 (SiO2)40 and Fe70 (SiO2)30 films. The small Fe grain size (<10 nm) and dilute Fe volume fraction (0.29–0.4) of these granular films produce tip coatings of low remanence and essentially zero coercivity, reduced by the superparamagnetic properties of these films. We have used these tips to obtain MFM images of the write field of the head with high spatial and magnetic-field resolution. In comparison to images taken using commercial Co85Cr15-coated tips, these MFM images show reduced tip memory effects and clearly delineate the gap field from the pole pieces.
Coupling And Attenuation Of Waves In Plates By Randomly Distributed Attached Impedances, Joseph A. Turner, Richard L. Weaver
Coupling And Attenuation Of Waves In Plates By Randomly Distributed Attached Impedances, Joseph A. Turner, Richard L. Weaver
Department of Engineering Mechanics: Faculty Publications
The average response of an infinite thin plate with statistically homogeneous attached random impedances is examined. The added impedances, which represent typical heterogeneities that might occur on complex shells, provide light coupling between the extensional, shear, and flexural waves. The mean plate response is formulated in terms of the Dyson equation which is solved within the assumptions of the first-order smoothing approximation, or Keller approximation, valid when the heterogeneities are weak. Scattering attenuations are derived for each propagation mode. It is shown that the attenuation of one wave type due to coupling to another is proportional to the modal density …
Women's Studies Spring 1996 Newsletter, Barbara Dibernard, Dristin Mapel-Bloomberg
Women's Studies Spring 1996 Newsletter, Barbara Dibernard, Dristin Mapel-Bloomberg
Women's and Gender Studies Program: Information and Materials
The University of Nebraska Foundation announces that an endowment has been created in support of the UNL Women's Studies Program. Named the Karen Dunning Fund for Women's Studies, the endowment will help to maintain general Women's Studies activities, as well as for student and faculty support.
Epilogue, Gargi Roysircar Sodowsky, James C. Impara
Epilogue, Gargi Roysircar Sodowsky, James C. Impara
Multicultural Assessment in Counseling and Clinical Psychology
The book has shown the use of a combination of approaches to understand the nature of a problem: traditional diagnosis and standardized assessment, cultural and racial explanations as alternative hypotheses, clinical judgement based on a decision-tree involving cross-cultural and indigenous frameworks, quantitative-qualitative methods of data analyses, and the use of multicultural paper-and pencil and projective tests. The attitudes and cognitive-affective tests presented or referenced in the book, in addition to being formally administered, could be used as springboards for collaborative discussions with clients and psychology trainees in order to gain a better understanding of their values and assumptions and, by …
Acuta Enews April 1996, Vol. 25, No. 4
Acuta Enews April 1996, Vol. 25, No. 4
ACUTA Newsletters
In This Issue
From the President
Pasadena Community College
Port by Every Pillow at WCU
Multi-Media at Sewanee
DC at a Glance
From ACUTA Headquarters
The Structural Gene Encoding Human Enterotoxigenic Escherichia Coli Pcfo20 Is Homologous To That For Porcine 987p, Gloria Inés Viboud, Gunhild Jonson, Evelyn A. Dean-Nystrom, Ann-Mari Svennerholm
The Structural Gene Encoding Human Enterotoxigenic Escherichia Coli Pcfo20 Is Homologous To That For Porcine 987p, Gloria Inés Viboud, Gunhild Jonson, Evelyn A. Dean-Nystrom, Ann-Mari Svennerholm
United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications
Putative colonization factor PCFO20 was recently identified in an enterotoxigenic Escherichia coli (ETEC) strain of serogroup O20 isolated from a child with diarrhea in Argentina. The gene encoding the structural subunit of PCFO20 fimbriae, fotA, was cloned from strain ARG-2 in the expression phage vector lambda ZAP Express. One positive clone, pGV29, that carried a 3.3-kb fragment was identified on the basis of fimbrillin production by using a monospecific rabbit anti-PCFO20 serum. Nucleotide sequencing of a 1.3-kb Sau3A-ClaI fragment of the subclone pGV292 containing the region coding for PCFO20 fimbrillin revealed two open reading frames of …
Water Current, Volume 28, No. 2, April 1996
Water Current, Volume 28, No. 2, April 1996
Water Current Newsletter
Speakers Clear Up "Muddy Mo" Perspectives at Conference
From the Director: Annual Water Conference Brings New Perspectives
Water Efforts Recognized at Awards Banquet
Global Warming: Researchers Find Plains at Risk for Climate Change
Free Environmental Sciences Software
Pesticide Guides, Maps Available
US Geological Survey Conducts Water Quality Assessment in Central Nebraska
Analysis Of Homogeneous Combustion In Monolithic Structures, Hendrik J. Viljoen
Analysis Of Homogeneous Combustion In Monolithic Structures, Hendrik J. Viljoen
Papers in Chemical Reactions
An analytical method is used to solve the problem of homogeneous combustion in a monolith. The solid phase acts as burner support. Heat is conducted much more effectively in the solid phase and preheating of the gas is more effective. The flame temperature exceeds the adiabatic value. Solutions are presented for different velocities and feed concentrations.
Theory Of Dipole Flow In Uniform Anisotropic Aquifers, Vitaly A. Zlotnik, Glenn Ledder
Theory Of Dipole Flow In Uniform Anisotropic Aquifers, Vitaly A. Zlotnik, Glenn Ledder
Department of Earth and Atmospheric Sciences: Faculty Publications
A theory of dipole flow is developed to model flow induced by a vertical circulation well consisting of injection and extraction chambers in a single borehole. Included in the theory are an analytical description of the kinematic flow structure around a vertical circulation well and the drawdown in the well chambers. Using Stokes’ stream function, simple criteria are derived to determine the region of intensive recirculation. This region extends (from the dipole center) approximately five distances between chamber centers in the radial direction and two distances between chamber centers in both vertical directions. The vertical scale does not depend on …
Nueva Especie Del Género Eurycotis Stal (Dictyoptera: Blattidae: Polyzosteriinae) Para Cuba, Esteban Gutierrez Cubria
Nueva Especie Del Género Eurycotis Stal (Dictyoptera: Blattidae: Polyzosteriinae) Para Cuba, Esteban Gutierrez Cubria
Insecta Mundi
A new endemic species of Eurycotis, E. perezassoi, predominantly found under stones associated with Pinus cubensis, is described from the eastern region of the Island of Cuba. Differences for separation of E. rhodae Gurney and E. lacernata Cabrera, from the new taxon, are given.
El Género Diastolinus (Coléoptera: Tenebrionidae: Pedinini) En Las Islas Caimán Con Descripción De Una Nueva Especie, Orlando H. Garrido, Esteban Gutierrez
El Género Diastolinus (Coléoptera: Tenebrionidae: Pedinini) En Las Islas Caimán Con Descripción De Una Nueva Especie, Orlando H. Garrido, Esteban Gutierrez
Insecta Mundi
A new species of Diastolinus, D. burtoni is described for the islands of Grand Cayman and Cayman Brac (Cayman Islands). Local forms are characterized by displaying a conspicuous enlargement of the front tibia, character not found in any Cuban species. However, Diastolirius dentipes (endemic to Grand Cayman) was also collected in Cayo Largo del Sur, Archipelago de Los Canarreos, Cuba. Therefore this species, could no longer be considered endemic to the Cayman Islands. Whether the original dispersion occurred from Cayman Island to Cayo Largo or vice versa, is hard to prove, nevertheless, the distinctive character of the dilatation of the …
Consideraciones Sobre El Genero Cyrtosoma (Coleoptera: Tenebrionidae: Cnodalonini) En Cuba Con La Descripción De Una Nueva Especie, Orlando H. Garrido, Esteban Gutierrez
Consideraciones Sobre El Genero Cyrtosoma (Coleoptera: Tenebrionidae: Cnodalonini) En Cuba Con La Descripción De Una Nueva Especie, Orlando H. Garrido, Esteban Gutierrez
Insecta Mundi
A new species of Cyrtosoma (subgenus Nesocyrtosoma Marcuzzi 1976) for Cuba is herein described. The tribe Cnodalonini is represented in the West Indies by the genus Cnodalon and Cyrtosoma. Cyrtosoma (Nesocyrtosorna) gebieni was described by Marcuzzi from "Sitio del Infierno", Pinar del Río. C. ruficornis Chevrolat was described from Cardenas. The following taxa of the genus Cyrtosoma (Nesocyrtosoma) are considered for Cuba: C. inflatum Marcuzzi 1976, type locality "Cuba"; C. tumefactum Marcuzzi 1976, type locality "Cuba"; C. gebieni Marcuzzi 1976, type locality "Sitio del Infierno", Cordillera de los Organos, Pinar del Río; C. paralellus Zayas 1988, type locality Cupeyal, …
Revisión Del Género Amphelictogon (Diplopoda: Polydesmida: Chelodesmidae) En Cuba, Antonio Perez-Asso
Revisión Del Género Amphelictogon (Diplopoda: Polydesmida: Chelodesmidae) En Cuba, Antonio Perez-Asso
Insecta Mundi
Resumen:
La revisión del material tipo y de especímenes colectados recientemente del género Amphelictogon (Diplopoda: Polydesmida: Chelodesmidae), ofrece los siguientes resultados: Una combinación nueva (Rhacophorus magrtus pasa a Amphelictogoli. magnus); seis sinónimos nuevos (A. rubripes, A. sygethus, A. guantanamanus, A. flavipes sinónimos de A. cubanus; A. bidens sinónimo de A. subterraneus; A. atricolor sinónimo de A. magnus); tres status nuevos (A. bahamiensis, A. dolius, A. pinetorum pasan a subspecies de A. subterraneus) y dos taxones en status incierto (A. juvenis, A. maculatus). Se describen e ilustran por primera vez los machos de …
Manuscript Typing Instructions
Manuscript Typing Instructions
Insecta Mundi
Our system of typesetting from typescripts submitted by authors involves the use of "Pagemaker." Diskettes submitted with format instructions cause extra work. Therefore we ask authors to remove all such instructions, and to submit their papers in manuscript format, i.e. as if you were typing without the aid of a computer. We then add, through the media of "Pagemaker" the format as we use it in INSECTA MUNDI. By following the instructions outlined below, we save time and money.
Acuta Enews March 1996, Vol. 25, No. 3
Acuta Enews March 1996, Vol. 25, No. 3
ACUTA Newsletters
In This Issue
From the President
Seeking a Wireless Solution
DC at a Glance
Telecom & Leg/Reg Listserves
From ACUTA Headquarters
Pod Network News, March 1996
Professional and Organizational Development Network in Higher Education: POD Network News
New Leadership for POD Core Committee
POD at AAHE
POD Conference October 17-20, 1996 Snowbird, Utah
From the President
Bright Ideas Addition
Position Announcements
New World Wide Web Pages
POD Authors
Resources
Spring and Summer Conferences
Revision And Phylogeny Of The Neotropical Genus Cnernida (Coleoptera: Scarabaeidae: Rutelinae), Mary Liz Jameson
Revision And Phylogeny Of The Neotropical Genus Cnernida (Coleoptera: Scarabaeidae: Rutelinae), Mary Liz Jameson
University of Nebraska State Museum: Entomology Papers
The scarab genus Cnemida includes eight species (including C. gigantea Jameson n. sp. from Colombia and C. tristriata Jameson n. sp. from Surinam) that inhabit tropical moist and premontane forests of South America, Central America, and Mexico. Keys to adults, diagnostic characters, descriptions, and distributions are presented. The larva of C. intermedia Bates is described and integrated into a key to larvae of the tribe Rutelini. A cladistic analysis among the species of Cnemida is based on 35 morphological characters and uses members of the genera Pelidnota and Rutela as outgroups. Four equally parsimonious cladograms are discussed.
El Genero Cerococcus En La Argentina (Homoptera: Cerococcidae), Maria Cristina Granara De Willink
El Genero Cerococcus En La Argentina (Homoptera: Cerococcidae), Maria Cristina Granara De Willink
Insecta Mundi
The new species Cerococcus lizeri is described; new geographical distribution for C. catenarius and new hosts for C. badius are given. A key with the 5 species from Argentina is here provided.
A Comparison Of Gross Pathology, Histopathology, And Mycobacterial Culture For The Diagnosis Of Tuberculosis In Elk(Cervus Elaphus), Elizabeth B. Rohonczy, Arumuga V. Balachandran, Thomas W. Dukes, Janet B. Payeur, Jack C. Rhyan, Dennis A. Saari, Terry L. Whiting, Susan H. Wilson, Jerald L. Jarnagin
A Comparison Of Gross Pathology, Histopathology, And Mycobacterial Culture For The Diagnosis Of Tuberculosis In Elk(Cervus Elaphus), Elizabeth B. Rohonczy, Arumuga V. Balachandran, Thomas W. Dukes, Janet B. Payeur, Jack C. Rhyan, Dennis A. Saari, Terry L. Whiting, Susan H. Wilson, Jerald L. Jarnagin
United States Department of Agriculture Wildlife Services: Staff Publications
Using the isolation of Mycobacterium bovis as the reference standard, this study evaluated the sensitivity, specificity and kappa statistic of gross pathology (abattoir postmortem inspection), histopathology, and parallel or series combinations of the two for the diagnosis of tuberculosis in 430 elk and red deer. Two histopathology interpretations were evaluated: histopathology I, where the presence of lesions compatible with tuberculosis was considered positive, and histopathology II, where lesions compatible with tuberculosis or a select group of additional possible diagnoses were considered positive. In the 73 animals from which M. bovis was isolated, gross lesions of tuberculosis were most often in …
Nebline, February 1996
NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County
Horticulture
Rural $ense
Family Living
4-H & Youth
Environmental Focus
Community & Leadership Development
Extension Calendar
Expanded Food and Nutrition Education Program (EFNEP)
Nebraska Association for Family and Community Education News
and other extension news and events
Acuta Enews February 1996, Vol. 25, No. 2
Acuta Enews February 1996, Vol. 25, No. 2
ACUTA Newsletters
In This Issue
From the President
The Michelangelo Virus
DC at a Glance
UCLA Switches on Its 6800
From ACUTA Headquarters
Infection Of Primary Human Fetal Astrocytes By Human Herpesvirus 6, Jun He, Micheline Mccarthy, Yi Zhou, Bala Chandran, Charles Wood
Infection Of Primary Human Fetal Astrocytes By Human Herpesvirus 6, Jun He, Micheline Mccarthy, Yi Zhou, Bala Chandran, Charles Wood
Nebraska Center for Virology: Faculty Publications
Human herpesvirus 6 (HHV-6) is a lymphotropic betaherpesvirus which productively infects human CD4+ T cells and monocytes. HHV-6 is the etiologic agent for exanthem subitum (roseola), and it is well-known that central nervous system complications occur frequently during the course of HHV-6-associated disease. In addition, HHV-6 has been associated with encephalitis or encephalopathy. However, very little is known about its tropism for neural cells. There are reports that HHV-6 may infect some glial cell lines, but whether it can infect any primary neural cells is not known. Our studies show that both HHV-6A (GS) and HHV-6B (Z-29) can infect …
Immunological Responses In The Mouse Host To A Cloned Antigen Of Taeniacrassiceps, Dolores E. Hill, Dante S. Zarlenga
Immunological Responses In The Mouse Host To A Cloned Antigen Of Taeniacrassiceps, Dolores E. Hill, Dante S. Zarlenga
United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications
Adult female Swiss-Webster mice were immunized either intraperitoneally (IP) or subcutaneously (SQ) with cyst fluid or a genetically engineered fusion protein, Taenia carassiceps antigen 2-maltose binding protein (TCA2-MBP) from Taenia crassiceps metacestodes, or with live, non-budding cysts SQ, and then challenged IP with T. crassiceps metacestodes and necropsied 9 weeks later. Numbers of peripheral blood eosinophils were increased after IP immunization, but were not increased after SQ immunization or with SQ cysts given before the challenge infection. Eosinophil numbers gradually decreased over the course of the experiment, and were not found in increased numbers in the blood or peritoneal cavity …
The Ethics Of Implementation: Institutional Remedies And The Lawyer's Role, Susan Poser
The Ethics Of Implementation: Institutional Remedies And The Lawyer's Role, Susan Poser
Nebraska College of Law: Faculty Publications
The issue addressed in this article is whether and to what extent a lawyer has an ethical responsibility to pursue implementation of the remedy in institutional reform litigation. Institutional reform litigation refers to cases in which an individual or class of individuals sues a large organization in order to vindicate constitutional or statutory rights. The types of cases with which this article is concerned are the "public law" type, such as school desegregation, prisoners' rights and patients' rights cases, although included under the rubric of institutional reform can be, inter alia, antitrust, reapportionment and bankruptcy cases. The implementation stage of …
Track Structure And Radiation Transport Model For Space Radiobiology Studies, F. A. Cucinotta, J. W. Wilson, Robert Katz, W. Atwell, G. D. Badhwar, M. R. Shavers
Track Structure And Radiation Transport Model For Space Radiobiology Studies, F. A. Cucinotta, J. W. Wilson, Robert Katz, W. Atwell, G. D. Badhwar, M. R. Shavers
Robert Katz Publications
Radiobiology experiments performed in space are deemed necessary for validation of risk-assessment methods. The understanding of space radiobiology experiments must combine knowledge of the space radiation environment, radiation transport, and models of biological response. The heavy ion transport code HZETRN has recently been combined with improved models of the galactic cosmic rays (GCR) and extensive comparisons made to measurements on the space shuttle with a tissue equivalent, proportional counter. HZETRN was also coupled with track-structure models of biological damage from heavy ions. Track-structure calculations using improved models of the radial dose distribution around the path of heavy ions provide a …
Acuta Enews January 1996, Vol. 25, No. 1
Acuta Enews January 1996, Vol. 25, No. 1
ACUTA Newsletters
In This Issue
From the President
SIUE $1.1 Million Phone Scam
NUI Implements ADSI Technology
Sewanee Campus Hooked up to Info Highway
ACUTA Listserves
From ACUTA Headquarters
Truncated Enterohemorrhagic Escherichia Coli (Ehec) O157:H7 Intimin (Eaea) Fusion Proteins Promote Adherence Of Ehec Strains To Hep-2 Cells, Marian L. Mckee, Alison D. O'Brien
Truncated Enterohemorrhagic Escherichia Coli (Ehec) O157:H7 Intimin (Eaea) Fusion Proteins Promote Adherence Of Ehec Strains To Hep-2 Cells, Marian L. Mckee, Alison D. O'Brien
Uniformed Services University of the Health Sciences
Intimin, the product of the eaeA gene in enterohemorrhagic Escherichia coli O157:H7 (EHEC), is required for intimate adherence of these organisms to tissue culture cells and formation of the attaching and effacing lesion in the gnotobiotic pig. Because of the importance of intimin in the pathogenesis of EHEC O157:H7 infection in this animal model, we began a structure-function analysis of EaeA. For this purpose, we constructed amino-terminal fusions of the intimin protein with six histidine residues to form two independent fusions. The longer fusion, RIHisEae, contained 900 of the 935 predicted amino acids and included all but the extreme amino …