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Philosophy And Purposes Of Distance Education, Dr. Janet K. Poley, Mr. Erik France Mar 1999

Philosophy And Purposes Of Distance Education, Dr. Janet K. Poley, Mr. Erik France

Nebraska Network 21: Publications

In considering how best to tackle this topic, I ultimately decided that in Nebraska the "best" approach is usually the "most straight forward" one. Given that much of the conversation about distance education is filled with myth and marketing hype, I decided what we could best contribute to the conversation would be the facts. While some of the other authors may have a better in-depth grasp of distance education opportunities and realities in Nebraska, Erik and I come as native Nebraskans with a good understanding of the history, economy, communities and cultures in our home state. My early faculty career …


Fish & Wildlife News: March/April 1999 Mar 1999

Fish & Wildlife News: March/April 1999

Fish and Wildlife News

Contents:

Key Positions Filled 2
Refuges Hit the Big Screen 5
Record Service Budget Proposed 6
All About Alligator Gar 7
Commercial Fishers Lend a Hand 8
Clark Commends Report Team 10
Federal Aid on the Information Superhighway 11
Floods Spur Conservation Initiative 13
Special: International Migratory Bird Day ‘99 15
Partners in Conservation Success 17
U.S., Japan Have Historic Meeting 20
Ambassadors of the Winged Variety 24
Birds Equal Bucks 25
Exploring Our Past: The History Pages 28
Fish and Wildlife...In Brief 31


Documentary Editing, Volume 21, Number 1, March 1999. Mar 1999

Documentary Editing, Volume 21, Number 1, March 1999.

Documentary Editing: Journal of the Association for Documentary Editing (1979–2011)

No abstract provided.


The Probe, Issue 198 – March 1999 Mar 1999

The Probe, Issue 198 – March 1999

The Probe: Newsletter of the National Animal Damage Control Association

NADCA benefits its members
Fifth Annual Wildlife Control Technology Instructional Seminar
Bob Willging Fund Established
Woodstream Drops Victor Traps
Activist Gets 7 Years in Prison
Egret Control Goes Badly
Foxes Threaten Plovers in Maine
Controversy Boils In Arizona Predator Hunt Contests

Abstracts from the 5th Annual Conference of The Wildlife Society

The Prospect of Using Oral Vaccination to Control Rabies in Raccoons in the Northeast -- D.H. Lein and L.L. Bigler
Past and Current Methods for Controlling Rabies in Wildlife -- S.B. Linhart and C.L. Brown
Elimination of Fox Rabies from Ontario, Canada -- CD. Maclnnes
A Computer Model for …


Nonspecific Interactions Alter Lipopolysaccharide Patterns And Protein Mobility On Sodium Dodecyl Sulfate Polyacrylamide Gels, Aidong Yuan, R L. Pardy, Catherine P. Chia Mar 1999

Nonspecific Interactions Alter Lipopolysaccharide Patterns And Protein Mobility On Sodium Dodecyl Sulfate Polyacrylamide Gels, Aidong Yuan, R L. Pardy, Catherine P. Chia

Papers in Microbiology

In testing whether bacterial lipopolysaccharide (LPS) was a natural substrate for an esterase from the soil amebae Dictyostelium discoideum, we observed altered banding patterns of the LPS and changed protein mobility on sodium dodecyl sulfate (SDS) polyacrylamide gels after incubation of LPS with the enzyme. The initial interpretation of these results was that the enzyme had removed ester-linked acyl chains from the LPS, leading to a change in its migration on gels. However, esterase inactivated by treatment with either dithiothreitol (DTT), heat, or SDS generated the same mobility shifts. Bovine serum albumin (BSA) also induced the same change in …


The Ade Annual Meeting Sessions Mar 1999

The Ade Annual Meeting Sessions

Documentary Editing: Journal of the Association for Documentary Editing (1979–2011)

Gateway to the West: Exploring Editorial Terrain--Documentary Editing: Yesterday, Today, and Tomorrow--Twenty Years of Documentary Editing: Personal Views--Present at the Creation: The Founding of the Association for Documentary Editing--Hooked on Editing: Moving on to New Projects


Liquid-Phase Catalytic Oxidation Of Unsaturated Fatty Acids , Hossein Noureddini, M Kanabur Mar 1999

Liquid-Phase Catalytic Oxidation Of Unsaturated Fatty Acids , Hossein Noureddini, M Kanabur

Papers in Biomaterials

Liquid-phase catalytic oxidation of oleic acid with hydrogen peroxide in the presence of various transition metal/metal oxide catalysts was studied in a batch autoclave reactor. Azelaic and pelargonic acids are the major reaction products. Tungsten and tantalum and their oxides in supported and unsupported forms were used as catalysts. Alumina pellets and Kieselguhr powder were used as supports for the catalysts. Tungsten, tantalum, molybdenum, zirconium, and niobium were also examined as catalysts. Tertiary butanol was used as solvent. Experimental results concluded that tungsten and tungstic oxide are more suitable catalysts in terms of their activity and selectivity. The rate of …


The Times, They Are A Changing: A Review Of Raising Children In A Socially Toxic Environment, Susan M. Sheridan, Richard J. Cowan Feb 1999

The Times, They Are A Changing: A Review Of Raising Children In A Socially Toxic Environment, Susan M. Sheridan, Richard J. Cowan

Department of Educational Psychology: Faculty Publications

Rarely does a book come along that we believe to be essential reading for all psychologists, educators, child care workers, physicians, clergy, and concerned community members. Garbarino’s Raising Children in a Socially Toxic Environment is one such book. In this book, Garbarino sets out to offer his views on what childhood ought to be, how children map their own worlds (thus define, themselves), what their basic needs are, the levels in which those needs are being met, as well as offer suggestions for ways in which readers can change the “toxic environment” to aid in the healthy development of children …


A Mutant Of Chlamydomonas Reinhardtii With Reduced Rate Of Photorespiration, Kensaku Suzuki, Tarlan G. Mamedov, Toraoyoshi Ikawa Feb 1999

A Mutant Of Chlamydomonas Reinhardtii With Reduced Rate Of Photorespiration, Kensaku Suzuki, Tarlan G. Mamedov, Toraoyoshi Ikawa

Papers in Biochemical Engineering

Photorespiration rates under air-equilibrated conditions (0.04% CO2 and 21% O2) were measured in Chlamydomonas reinhardtii wild-type 2137, a phosphoglycolate-phosphatase-deficient (pgpl) mutant and a suppressor double mutant (7FR2N) derived from the pgpl mutant. In both cells grown under 5% CO2 and adapted air for 24 h in the suppressor double mutant, the maximal rate of photorespiration (phosphoglycolate synthesis) was only about half of that in either the wild type or the pgpl mutant (18-7F) cells. In the progeny, the reduced rate of photorespiration was accompanied by increased photosynthetic affinity for inorganic carbon and the capacity for growth …


Anglo (Mis)Understandings Of Latino Newcomers: A North Georgia Case Study, Edmund T. Hamann Feb 1999

Anglo (Mis)Understandings Of Latino Newcomers: A North Georgia Case Study, Edmund T. Hamann

Department of Teaching, Learning, and Teacher Education: Faculty Publications

This paper examines how Anglos in a small north Georgia city imagined, or conceptualized, Hispanics during the late 1990s as thousands of Spanish-speaking immigrant newcomers transformed the community’s demography. Based on two years of ethnographic research, the paper outlines the local play of several macro-social dynamics, such as businesses’ externalization of indirect costs, the ethnic segmentation of the work force, the use of sojourner labor, and the role of mediating institutions. The paper uses these dynamics to explain the emergence and sustenance of what Suárez-Orozco (1998) calls the “pro-immigration” and “anti-immigration scripts” and illustrates how these can be used to …


Levels Of Major Selenoproteins In T Cells Decrease During Hiv Infection And Low Molecular Mass Selenium Compounds Increase, Vadim Gladyshev, Thressa C. Stadtman, Dolph L. Hatfield, Kuan-Teh Jeang Feb 1999

Levels Of Major Selenoproteins In T Cells Decrease During Hiv Infection And Low Molecular Mass Selenium Compounds Increase, Vadim Gladyshev, Thressa C. Stadtman, Dolph L. Hatfield, Kuan-Teh Jeang

Vadim Gladyshev Publications

It has been observed previously that plasma selenium and glutathione levels are subnormal in HIV-infected individuals, and plasma glutathione peroxidase activity is decreased. Under these conditions the survival rate of AIDS patients is reduced significantly. In the present study, using 75Se-labeled human Jurkat T cells, we show that the levels of four 75Se-containing proteins are lower in HIV-infected cell populations than in uninfected cells. These major selenoproteins migrated as 57-, 26-, 21-, and 15-kDa species on SDS/PAGE gels. In our earlier studies, the 57-kDa protein was purified from T cells and identified as a subunit of thioredoxin reductase. …


Acuta Enews February 1999, Vol.28, No. 2 Feb 1999

Acuta Enews February 1999, Vol.28, No. 2

ACUTA Newsletters

In This Issue

Call for Nominations: ACUTA Board of Directors 1999-2000

Levine, McDonalds to Speak at Seminars

Network Challenges for the 21st Century

Overheard on the Listserve

Board Report

Distance Ed Institutes

DC at a Glance

Welcome New Members

Positions Available

A Leader Is a Big Thinker- The Magnet That Attracts Others


Bovine Follicular Dynamics, Oocyte Recovery,And Development Of Oocytes Microinjected With A Green Fluorescent Protein Construct, M S. Chauhan, S Nadir, T L. Bailey, A W. Pryor, S P. Butler, D R. Notter, William H. Velander, F C. Gwazdauskas Jan 1999

Bovine Follicular Dynamics, Oocyte Recovery,And Development Of Oocytes Microinjected With A Green Fluorescent Protein Construct, M S. Chauhan, S Nadir, T L. Bailey, A W. Pryor, S P. Butler, D R. Notter, William H. Velander, F C. Gwazdauskas

Papers in Biotechnology

The present study was carried out to 1) evaluate the viability of in vitro fertilized zygotes after microinjection of DNA, 2) assess the influence of oocyte quality upon the development rate of embryos when injected with DNA, and 3) determine the integration frequency of green fluorescent protein DNA into microinjected embryos. Oocytes were aspirated from ovaries of nine nonlactating Holsteins and were categorized into grades A, B, C, and D. At 16 h after in vitro fertilization, approximately half of the pronuclear stage presumptive zygotes were classified as having 1 pronucleus or 2 pronuclei, and they were microinjected with DNA …


Child Physical Abuse, David J. Hansen, Georganna Sedlar, Jody E. Warner-Rogers Jan 1999

Child Physical Abuse, David J. Hansen, Georganna Sedlar, Jody E. Warner-Rogers

Department of Psychology: Faculty Publications

Widespread attention to the problem of child physical abuse has increased dramatically in recent decades. Extensive research evidence has described child physical abuse as a complex, multidimensional phenomenon that is best assessed by procedures using multiple modalities (e.g., interview, self-report, direct observation) that address multiple content areas. Comprehensive assessment is essential for identifying risk and occurrence of abuse, guiding the focus or direction of treatment, as well as monitoring treatment efficacy and outcome, all of which may be disseminated to interested parties as appropriate (e.g., CPS, judicial system, school, other treatment providers). Increasingly specific and relevant procedures have become available …


The Ecological Validity Of Jury Simulations: Is The Jury Still Out?, Brian H. Bornstein Jan 1999

The Ecological Validity Of Jury Simulations: Is The Jury Still Out?, Brian H. Bornstein

Department of Psychology: Faculty Publications

Despite the need to assess the ecological validity of jury simulation research before generalizing from simulations to the behavior of real jurors, surprisingly little jury research has directly addressed issues of validity. The present paper reviews the extant research on two aspects of the validity question—specifically, research that has compared different samples of mock jurors, and research that has manipulated the medium of trial presentation. In addition, jury simulation research published in the first 20 years of Law and Human Behavior is analyzed with respect to these variables. The majority of simulations used student-jurors and presented the trial in written …


Measuring Fractional Cover And Leaf Area Index In Arid Ecosystems: Digital Camera, Radiation Transmittance, And Laser Altimetry Methods, Michael A. White, Gregory P. Asner, Ramakrishna R. Nemani, Jeff L. Privette, Steven W. Running Jan 1999

Measuring Fractional Cover And Leaf Area Index In Arid Ecosystems: Digital Camera, Radiation Transmittance, And Laser Altimetry Methods, Michael A. White, Gregory P. Asner, Ramakrishna R. Nemani, Jeff L. Privette, Steven W. Running

United States National Aeronautics and Space Administration: Publications

Field measurement of shrubland ecological properties is important for both site monitoring and validation of remote sensing information. During the May 1997 NASA Earth Observing System Jornada Prototype Validation Exercise, we calculated plot-level plant area index, leaf area index, total fractional cover, and green fractional cover with data from four instruments: (1) a Dycam Agricultural Digital Camera (ADC), (2) a LI-COR LAI-2000 plant canopy analyzer, (3) a Decagon sunfleck Ceptometer, and (4) a laser altimeter. Estimates from the LAI-2000 and Ceptometer were very similar (plant area index 0.3, leaf area index 0.22, total fractional cover 0.19, green fractional cover 0.14), …


Integration Of Hydroxyapatite Concentration Of Bacteria And Seminested Pcr To Enhance Detection Of Salmonella Typhimurium From Ground Beef And Bovine Carcass Sponge Samples, Elaine D. Berry, Gregory R. Siragusa Jan 1999

Integration Of Hydroxyapatite Concentration Of Bacteria And Seminested Pcr To Enhance Detection Of Salmonella Typhimurium From Ground Beef And Bovine Carcass Sponge Samples, Elaine D. Berry, Gregory R. Siragusa

Roman L. Hruska U.S. Meat Animal Research Center: Reports

Hydroxyapatite (HA) concentration of bacteriafiom ground beef and bovine carcass sponge samples was examined as a method to enhance the PCR detection of Salmonella typhimurium in these samples. Ground beef and carcass sponge samples were inoculated with progressively lower levels of S. typhimurium. Concentrated (10% HA) and unconcentrated samples were prepared for PCR after 0, 2, 3, or 4 h of nonselective enrichment. Without HA concentration and enrichment, Salmonella in ground beef was not detected by seminested PCR, even when present at levels of 103 cells/mL in the 1:10 ground beef homogenates. However, when bacteria in these samples were …


Statistical Validation Of The Track-Dilution Plating Method From Ground Beef And Carcass Surface Samples, Gregory R. Siragusa Jan 1999

Statistical Validation Of The Track-Dilution Plating Method From Ground Beef And Carcass Surface Samples, Gregory R. Siragusa

Roman L. Hruska U.S. Meat Animal Research Center: Reports

A rapid and easy method of obtaining a viable culture count, the Track-Dilution method, was evaluated and compared to Spiral Plating to obtain viable culture counts from ground beef samples and beef carcass su$ace tissue samples inoculated with salmonellae or Escherichia coli OIS7:H7. Based on the statistical analysis of the dataset (n = 125 samples), the Track-Dilution method results were not diflerent (P > 0.1) than those obtainedfiom Spiral Plating. Linear correlation of the scatterplot of Spiral Plating and Track-Dilution indicated a high level of agreement between these two methocis (9 = 0.97). The Track-Dilution method was a valid method for …


How The Public Views The State Courts A 1999 National Survey, Frank A. Bennack Jr. Jan 1999

How The Public Views The State Courts A 1999 National Survey, Frank A. Bennack Jr.

University of Nebraska Public Policy Center: Publications

The survey covered four broad areas: access to the courts, timeliness of court decisions, fairness of judicial decision-making, independence and responsiveness of the courts to the public and to changing conditions in society.

The questions came at the issues from many angles, but when all the figures were totaled and all the comments considered, the courts came out just okay on average.

This conclusion is a mix of high, medium and low marks across different categories. Sometimes there was broad consensus and other times views differed widely by race, ethnic group, income and other factors.


Influence Of Aqueous And Solid-Phase Fe(Ii) Complexants On Microbial Reduction Of Crystalline Iron(Iii) Oxides, Matilde Urrutia, Eric E. Roden, John M. Zachara Jan 1999

Influence Of Aqueous And Solid-Phase Fe(Ii) Complexants On Microbial Reduction Of Crystalline Iron(Iii) Oxides, Matilde Urrutia, Eric E. Roden, John M. Zachara

United States Department of Energy: Publications

The influence of aqueous (NTA and EDTA) and solidphase (aluminum oxide, layer silicates) Fe(II) complexants on the long-term microbial reduction of synthetic goethite by Shewanella alga strain BrY was studied. NTA enhanced goethite reduction by promoting aqueous Fe(II) accumulation, in direct proportion to its concentration in culture medium (0.01-5 mM). In contrast, EDTA failed to stimulate goethite reduction at concentrations e1 mM, and 5 mM EDTA enhanced the final extent of reduction by only 25% in relation to nonchelator controls. The minor effect of EDTA compared to NTA, despite the greater stability of the Fe(II)- EDTA complex, likely resulted from …


Application Of Polymerase Chain Reaction To The Diagnosis Of Infectious Diseases, David N. Fredricks, David A. Relman Jan 1999

Application Of Polymerase Chain Reaction To The Diagnosis Of Infectious Diseases, David N. Fredricks, David A. Relman

United States Department of Veterans Affairs: Publications

PCR and other sequence-based microbial detection methods, once considered to be only research tools, are being used increasingly in the clinical microbiology laboratory. As this technology expands into the clinical arena, clinicians will need to learn its advantages and limitations so that sound judgments can be made. Astute clinicians know that results of blood culture reports, whether positive or negative, must be interpreted using an understanding of the test employed and an assessment of the clinical scenario. Similarly, infectious diseases practitioner will need to expand their understanding of PCR-based diagnostics so that these powerful tests are used appropriately.

It is …


Effect Of Disorder On The Magnetic Properties Of Smco5, Diandra Leslie-Pelecky, R.L. Schalek Jan 1999

Effect Of Disorder On The Magnetic Properties Of Smco5, Diandra Leslie-Pelecky, R.L. Schalek

Diandra Leslie-Pelecky Publications

Mechanical milling of initially ordered ferromagnetic SmCo5 produces dramatic increases in coercivity after short (15 min to 1 h) milling times, accompanied by remanence ratios on the order of 0.7 and shifted hysteresis loops. X-ray diffraction shows that milling induces both chemical and structural disorder. The hysteresis-loop shift is continuous and nonlinear with temperature over the range 5–300 K. The high coercivities are attributed to the formation of a nanostructure consisting of crystalline SmCo5 regions separated by a disordered interphase.


Hands-On Computer Use In Science Classrooms: The Skeptics Are Still Waiting, Alan Runge, Amy N. Spiegel, Lisa M. Pytlikzillig, Steven Dunbar, Robert Fuller, Glenn Sowell, David W. Brooks Jan 1999

Hands-On Computer Use In Science Classrooms: The Skeptics Are Still Waiting, Alan Runge, Amy N. Spiegel, Lisa M. Pytlikzillig, Steven Dunbar, Robert Fuller, Glenn Sowell, David W. Brooks

Robert G. Fuller Publications

Frank Collea was a friend of Robert Fuller and David Brooks, and a mentor to Brooks. We miss him for his energy, his enthusiasm for teaching science, and his perception about how to improve science education. Frank Collea was not a big fan of using computers in instruction. Frank was neither an advocate of using computers to deliver instruction, nor an advocate of teaching their use as professional tools. Indeed, he thought that most of those of us who advocate computer use make assertions that are unwarranted. A decade ago, desktop computers were beginning to appear in colleges and universities …


G99-1384 Gray Leaf Spot Of Corn, James Stack Jan 1999

G99-1384 Gray Leaf Spot Of Corn, James Stack

University of Nebraska-Lincoln Extension: Historical Materials

All corn hybrids and inbreds are susceptible to gray leaf spot in varying degrees. This NebGuide discusses the symptoms, impacts and management of this disease.

Corn is grown throughout Nebraska on over 8 million acres of land; approximately 5 million acres are irrigated. The market is segmented into seed corn, field corn and specialty corns (e.g., high oil, high amylose and white corn). Field corn represents the largest portion of the acreage grown. To varying degrees, all corn hybrids and inbreds are susceptible to gray leaf spot disease.

Gray leaf spot is a significant disease worldwide. It has been present …


Adaptive Harvest Management Working Group, Fred Johnson Jan 1999

Adaptive Harvest Management Working Group, Fred Johnson

United States Fish and Wildlife Service: Publications

AHM Implementation: Status and Issues (Fred Johnson)

Pacific Flyway Report (Dan Yparraguirre, Tom Aldrich, and Bob Trost)

Central Flyway Report (Mike Johnson, Jim Gammonley, and Dave Sharp)

Mississippi Flyway Report (Dale Humburg, Scott Baker, and Ken Gamble)

Atlantic Flyway Report (Bryan Swift, Gary Costanzo, and Jerry Serie)

Modeling and Adaptive Management of American Black Duck Populations (Mike Conroy)

Estimating optimal waterfowl harvest decisions using the genetic algorithm (Clinton T. Moore, Michael J. Conroy, Kevin Boston, and Walter D. Potter)

Communications Update (Dave Case and the Communications Team)

Communications Workshop (Fred Johnson and Working Group)

Current Conditions and Outlook for Breeding …


The Probe, Issue 196 – January 1999 Jan 1999

The Probe, Issue 196 – January 1999

The Probe: Newsletter of the National Animal Damage Control Association

A History of the Wildlife Services Program -- Donald W. Hawthorne, Gary L. Nunley, and Vivian Prothro, USDA-APHIS-Wildlife Services, Oklahoma and Texas
Position Available: Postdoctoral Research Associate, U.S.D.A. Agricultural Research Service, Stuttgart National Aquaculture Research Center (SNARC) in Stuttgart, Arkansas.
Book Review: "Being Kind to Animal Pests: A No-Nonsense Guide to Humane Animal Control with Cage Traps" by Steve Meyer (self-published), Garrison, I A 1991. 132 pages.
Position Announcement: Post Graduate Researcher, Dept. of Wildlife, Fish and Conservation Biology, University of California, Davis
Scmidt Appointed to AVMA Panel on Euthanasia

Abstracts from the 5th Annual Conference of The Wildlife Society, …


Fish & Wildlife News: January 1999 (Special Edition: National Wildlife Refuge System Conference) Jan 1999

Fish & Wildlife News: January 1999 (Special Edition: National Wildlife Refuge System Conference)

Fish and Wildlife News

Contents:

High Altitude, High Spirits 2
Honoring a Congressman 5
Media Baron Speaks Up for Wildlife 6
“No More Roads,” Says Babbitt 6
Panelists Address Diversity Issues 9
Barry Describes the “State of the System” 13
Another Roosevelt Who Loves Refuges 15
Winning Managers Share Their Thoughts 17
Results of Breakout Sessions 22
Director Lauds a New Era 23
AD Dan Ashe Looks Back 26
Refuges Chief Coleman Looks Ahead 27


Fish & Wildlife News: January/February 1999 Jan 1999

Fish & Wildlife News: January/February 1999

Fish and Wildlife News

Contents:

Anne Badgley in as Region 1 RD 2
Service Releases More Mexican Wolves 4
LE Busts Coral Smuggling Ring 5
Service Embraces Migratory Bird Initiatives 6
A New Concept in Beef 8
Interior Plans 150th Bashes Year-Round 9
Bringing the Hatchery to the Volunteers 10
Cranes Draw Crowds in New Mexico 11
Tribute to Region 1 Employees Killed on Duty 12
Team Implements Ecosystem Approach 15
Fighting Invasives in the Ohio River 16
Hollywood Comes to a Refuge 18
Rare Fish Makes a Comeback 23
Student Leaders Take D.C. by Storm 26


Small Farming Systems For The Midwest And Reintegrating Agriculture And Community In The Midwest, Richard Olson, Lisa Bauer Jan 1999

Small Farming Systems For The Midwest And Reintegrating Agriculture And Community In The Midwest, Richard Olson, Lisa Bauer

CARI Extension and Education Materials for Sustainable Agriculture

Part I: Small Farming Systems for the Midwest Small farms are real farms. 1 John Ikerd

A time to act for family-sized farms. 7 Chuck Hassebrook

Successful systems for market gardening 13 Lynn Byczynski

Hogs, hoop houses, and holistic management: A diversified crop/livestock farm 23 Tom Frantzen

Trees and herbs: A multi-storied agricultural system for southeastern Iowa 29 Tom Wahl, Kathy Dice

Integrating quality of life, economic and environmental issues: An agroecosystem analysis of Amish farming 39 Deborah Stinner, Richard Moore, Benjamin Stinner, Fred Hitzhusen

Adding value: Pastured-poultry, direct marketing and other strategies 47 Muriel Barrett

Integrating management-intensive grazing with …


Hydrogen-Deuterium Exchange And Ultraviolet Resonance Raman Spectroscopy Of Bacteria In A Complex Food Matrix, Gregory P. Harhay, Gregory R. Siragusa Jan 1999

Hydrogen-Deuterium Exchange And Ultraviolet Resonance Raman Spectroscopy Of Bacteria In A Complex Food Matrix, Gregory P. Harhay, Gregory R. Siragusa

Roman L. Hruska U.S. Meat Animal Research Center: Reports

The rapid identification and quantification of pathogenic foodborne bacteria is a national research priority. Data collected with a new spectrochemical method suggests that resonance Raman spectroscopy can rapidly and selectively identify and quantify bacteria in foods and other complex biomatrices. This method utilizes hydrogen-deuterium exchange (HDE) to resolve the spectral fingerprints of individual components in the overlapped regions of the ultraviolet resonance Raman spectra of heterogeneous samples. We illustrate this concept with HDE-induced changes in the spectra of bacteria and beef carcass wash samples. The data presented here suggest that the combination of ultraviolet resonance Raman spectroscopy and HDE can …