Digital Commons Network^™

The Role Of Chitinase Production By Stenotrophomonas Maltophilia Strain C3 In Biological Control Of Bipolaris Sorokiniana, Zhongge Zhang, Gary Y. Yuen

Department of Plant Pathology: Faculty Publications

The role of chitinase production by Stenotrophomonas maltophilia strain C3 in biological control of leaf spot on tall fescue (Festuca arundinacea), caused by Bipolaris sorokiniana, was investigated in vitro and in vivo. The filtrate of a broth culture of C3, with chitin as the carbon source, was separated into fractions. A high molecular-weight fraction (>8 kDa) was chitinolytic and more inhibitory than a low-molecularweight, nonchitinolytic fraction to conidial germination and hyphal growth by B. sorokiniana and to leaf spot development. A protein fraction derived by ammonium sulfate precipitation and a chitinase fraction purified by chitin affinity …

Isovolumic Regulation In Nervous Tissue: A Novel Mechanism Of Cell Volume Regulation, O. Quesada, R. Franco, K. Hernandez-Fonseca, K. Tuz

School of Veterinary and Biomedical Sciences: Faculty Publications

Cell volume regulation is a property present in most animal cell lineages that allows them to recover their original volume after events of swelling or shrinkage. Such events can be caused by changes in external osmolarity or to osmotic gradients generated during normal cell functioning.4,6 The mechanism of cell volume regulation involves transmembrane fluxes of osmotically active solutes in the necessary direction to counteract the net gain or loss of intracellular water.9 The process through which cells recover their normal volume after swelling is named Regulatory Volume Decrease (RVD). This consists of the efflux of inorganic osmolytes, such as K …

Effect Of Sample Complexity On Quantification Of Analytes In Aqueous Samples By Near-Infrared Spectroscopy, Mark R. Riley, Mark A. Arnold, David W. Murhammer

Department of Agricultural and Biological Systems Engineering: Faculty Publications

This study was undertaken to quantitate the impact of increasing sample complexity on near-infrared spectroscopic (NIRS) measurements of small molecules in aqueous solutions with varying numbers of components. Samples with 2, 6, or 10 varying components were investigated. Within the 10-component samples, three analytes were quantified with errors below 6% and seven of the analytes were quantified with errors below 10%. An increase in the number of varying components can substantially increase the error associated with measurement. A comparison of measurement errors across sample sets, as gauged by the standard error of prediction (SEP), reveals that an increase in the …

Role Of Esterase Gp70 And Its Influence On Growth And Development Of Dictyostelium Discoideum, Aidong Yuan, Catherine P. Chia

Papers in Microbiology

Gp70 is an esterase originally called crystal protein because of its presence in crystalline structures in aggregation-competent Dictyostelium discoideum cells. Although postulated to break down spore coats, the function of gp70 in vivo was incompletely investigated. Our immunolocalization and biochemical studies of vegetative D. discoideum amoebae show that gp70 was recruited to phagosomes and found in lysosomes. Purified gp70 was effective at hydrolyzing naphthyl substrates with acyl chains typical of lipids and lipopolysaccharides, indicating that the gp70 was involved in digesting endocytosed molecules. The activity of purified gp70 was inhibited by reductants that retarded its electrophoretic mobility and verified the …

Natural And Anthropogenic Influences On The Distribution Of The Threatened Neosho Madtom In A Midwestern Warmwater Stream, Mark L. Wildhaber, Vernon M. Tabor, Daniel W. Mulhern, Kenneth L. Powell, Scott P. Sowa

United States Geological Survey: Staff Publications

We attempted to discern the contributions of physical habitat, water chemistry, nutrients, and contaminants from historic lead-zinc mining activities on the riffle-dwelling benthic fish community of the Spring River, a mid western warm water stream that originates in Missouri and flows into Kansas and Oklahoma. The Spring River has a fish community that includes the Neosho madtom Noturus placidus, a species federally listed as threatened. Although anthropogenic factors such as contaminants limited populations and densities of fishes, an integrated assessment of natural and anthropogenic factors was necessary to effectively estimate the influence of the latter. Fish populations in the …

Haying, Tillage, And Nitrogen Fertilization Influences On Infiltration Rates At A Conservation Reserve Program Site, Brian J. Wienhold, Donald L. Tanaka

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Effect of haying (hayed or not hayed prior to tillage), tillage (no-tillage, minimum tillage, or conventional tillage), and N fertilization (0 or 67 kg ha^‒1) on surface infiltration rates, Q(h), was evaluated for Conservation Research Program (CRP) site conversion. Soils included Amor loam (fine-loamy, mixed, superactive, frigid Typic Haplustoll) and Cabba silt loam (loamy, mixed, superactive, calcareous, frigid, shallow Typic Ustorthent). In reference plots Q(h) increased from 1995 to 1997 (27.2 ± 3.2 vs. 36.4 ± 2.9 mm h^‒1 at 50-mm tension, 10.9 ± 1.2 vs. 20.6 ± 1.4 mm h^‒1 at 100-mm tension, and 4.1 …

Oxygen Metabolism In Plantlbacteria Interactions: Characterization Of The Oxygen Uptake Response Of Plant Suspension Cells, C. Jacyn Baker, E. W. Orlandi, Kenneth L. Deahl

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

In recent years the accumulation of reactive oxygen species (ROS) has been studied in plant cell suspension systems treated with bacterial pathogens. However, the associated utilization of molecular oxygen has not been well characterized. Using a multi-electrode oxygen analyser, the rates of oxygen consumption by tobacco cells during bacterial interactions were monitored. Heat-killed (HK) bacteria, which initiate an immediate ROS response in plant cells, were used as an elicitor to avoid complications of oxygen consumption by viable bacteria. An increase in oxygen uptake by the tobacco cells occurred within 4 min after addition of HK-bacteria and lasted for about 10 …

Quail Iv: Proceedings Of The Fourth National Quail Symposium (May 6-9, 1997 : Florida State University, Tallahassee, Florida), Leonard A. Brennan, William E. Palmer, Loren W. Burger Jr., Teresa L. Pruden

Galliformes Specialist Group and Affiliated Societies: Conference Proceedings

Fourth Herbert L. Stoddard, Sr. Memorial Game Bird Lecturer: Dr. G. R. Potts, Leonard A. Brennan

FOURTH STODDARD MEMORIAL GAME BIRD LECTURE

Using the Scientific Method to Improve Game Bird Management and Research: Time, G. R. Potts

QUAIL POPULATION RESPONSES TO HABITAT MANAGEMENT AND CHANGE

Habitat Use by Breeding Northern Bobwhites in Managed Old-Field Habitats in Mississippi, Jimmy D. Taylor, II and Loren W. Burger, Jr.

Habitat Management for Northern Bobwhites in Wisconsin: A Long-Term Assessment, LeRoy R. Petersen, Robert T. Dumke, and Barbara F. Duerksen

Effects of Filter Strips on Habitat Use and Home Range of Northern Bobwhites on …

Faithful To The Constitution: The Roadblock For Nebraska's Schools, Richard E. Shugrue

Nebraska Law Review

For six decades, American schools have been the major battleground in the controversy surrounding the doctrine of separation of church and state.There are huge problems and tensions among the warring parties in religion-school cases, and resort to state constitutional law will not make them disappear. The determined federal judiciary chooses to snub history, tradition, and settled state law to inject its version of civic religion back into the schools. Courts ought to undertake careful examination of the history, language, and experience before embracing a one-size-fits-all rule. For more than a century, states such as Nebraska have fought the battle over …

Understanding The Dynamics Of Child Maltreatment: Child Harm, Family Healing, And Public Policy (Discussant's Commentary), Ross A. Thompson

Department of Psychology: Faculty Publications

For almost a half-century, the Nebraska Symposium on Motivation has profiled many of the evolving themes and issues at the heart of psychology. It has also documented, less directly, changes in society. When the symposium was inaugurated in 1951, child maltreatment was a sad reality of life for many children, but it was not a topic of considerable professional attention. With the identification of the "battered child syndrome" (Kempe, Silverman, Steele, Droegemueller, & Silver, 1962) in the early 1960s, however, professional concern with the plight of abused and neglected children quickly grew. National attention to child maltreatment also increased as …

High Coercivity Longitudinal Recording Media And Method For Its Preparation, David J. Sellmyer, Chun-Ping Luo

Department of Physics and Astronomy: Faculty Publications

A high coercivity longitudinal recording media comprising FePt or similar thin films that are vacuum deposited by RF and DC sputtering. The as-deposited multilayerd films are subjected to vacuum annealing. Due to the formation of the ordered phases of FePt or the like, which have very high crystal anisotropy, the annealed FePt films have large coercivity and fine grain size, suitable for use as ultra-high density magnetic recording media.

Dissertations And Theses Sponsored By The Department Of Sociology In The University Of Nebraska-Lincoln 1905-1999: Alphabetical And Chronological Lists, Michael R. Hill

Department of Sociology: Faculty Publications

The year 2000 marks the centennial of the formal departmental organization of sociology at the University of Nebraska. The compilations presented here recognize and celebrate the achievements of hundreds of graduate students, my fellow alumni, who have completed masters theses and doctoral dissertations under the auspices of the Department of Sociology. These student works are constructive, often innovative additions to the advancement of knowledge, and several have been abridged in journal articles or published as books (cf., Hill 1988b). The doctoral dissertation, in particular, is a major rite de passage in the transition from student to intellectual (Deegan and Hill …

Student Collaboration In Faculty Development: Connecting Directly To The Learning Revolution, Milton D. Cox, D. Lynn Sorenson

To Improve the Academy: A Journal of Educational Development

Although faculty developers have worked successfully with faculty to focus on ways to enhance learning and listen to student voices, developers have rarely formed partnerships with students. This chapter reviews established practices involving students directly in faculty development, such as student observer/consultant programs. It also describes the nature, dynamics, and outcomes of some interesting new programs involving students in teaching development activities, thereby empowering students to join developers as change agents ofcampus culture. Finally, this chapter raises issues for faculty developers to reflect on as they consider establishing direct connections-partnerships-with students.

States Of Being In The Dark Removal And Survival In Linda Hogan's Mean Spirit, Eric Gary Anderson

Great Plains Quarterly (through 2013)

In 1990, Chickasaw poet Linda Hogan published her first novel, Mean Spirit, a well-received and yet controversial account of the Osage oil boom of the 1920s and the subsequent rash of criminal conspiracies and murders that has come to be known as the "Osage Reign of Terror."² The story she tells is often grim and violent; it returns again and again to "states of being in the dark" (44), which Hogan depicts as a wide-ranging confusion and uncertainty that afflicts many of her characters and demonstrates the period's turbulence to her readers. But the story is also resolutely …

Review Of West-Fever By Brian W. Dippie, Joni L. Kinsey

Great Plains Quarterly (through 2013)

West-Fever is a large-format, glossy book published to commemorate the tenth anniversary of the Autry Museum of Western Heritage. In its first decade the Autry has emerged as a growing presence in western history and art through an impressive record of acquisitions. (Its collection now numbers some 40,000 pieces, including some truly major objects: John Gast's 1872 painting, American Progress, and Thomas Moran's Mountain of the Holy Cross, 1874, to name just two.) The museum has sponsored and participated in a variety of important exhibitions, and now, it seems, it aspires to enhance its reputation through a new …

Changing The Pitch Americanism, Athleticism, And The Development Of Legion Baseball In Nebraska, Kent M. Krause

Great Plains Quarterly (through 2013)

Since its inception in 1928, American Legion Junior Baseball has been popular in Nebraska. Although originally started to advance the Legion's ideological agenda of Americanism, the primary factor in the success of Junior Baseball has been an active level of support from the citizens and businesses in the state's communities. The Legion's program acquired and maintained such support because of the variety of functions it served in towns and cities across the state. First and foremost, Junior Baseball teams were important sources of civic pride to Nebraskans who enthusiastically backed their local boys. The program also provided local businesses with …

Predicting The Probability Of Detecting Organochlorine Pesticides And Polychlorinated Biphenyls In Stream Systems On The Basis Of Land Use In The Pacific Northwest, Usa, Robert W. Black, Alan L. Haggland, Frank D. Voss

United States Geological Survey: Staff Publications

We analyzed streambed sediment and fish tissue (Cottus sp.) at 30 sites in the Puget Sound and Willamette basins in Washington and Oregon, USA, respectively, for organochlorine pesticides and polychlorinated biphenyls (PCBs). The study was designed to determine the concentrations of organochlorine pesticides and PCBs in fish tissue and sediment by land use within these basins and to develop an empirical relation between land use and the probability of detecting these compounds in fish tissue or sediment. We identified 14 organochlorines in fish tissue and sediment; three compounds were unique to either fish tissue or sediment samples. The …

A Cox2 Molecular Phylogeny Of The Peronosporomycetes, Deborah S.S. Hudspeth, Steven A. Nadler, Michael E.S. Hudspeth

Harold W. Manter Laboratory of Parasitology: Faculty Publications

The evolutionary history of the mitochondrial COX2 locus has been used to infer the phylogenetic relationships among 15 peronosporomycete and a hyphochytriomycete species. This molecular phylogenetic analysis at both the ordinal and generic levels provides strong evidence for the recognition of the Saprolegniomycetidae and the Peronosporomycetidae as natural groups, and for the monophyly of the Saprolegniales, Leptomitales and Pythiales. A three amino acid insertion/deletion event (indel) has been identified as a putative synapomorphy for the Saprolegniales. Parsimony mapping of 12 morphological and biochemical characters on the COX2 molecular phylogeny yields an hypothesis for peronosporomycete ancestral states and shared-derived features.

Soil-Moisture Preferences And Soil-Use Behaviors Of Northern Pocket Gophers, Ray T. Sterner

United States Department of Agriculture Wildlife Services: Staff Publications

Factors affecting soil-contact and –manipulation behaviors of pocket gophers (Thomomys and Geomys spp.) are poorly understood. Delineation of these behaviors is crucial to development of new repellent systems that seek to exploit the fossorial activity of these rodents. In a laboratory study involving northern pocket gophers (Thomomys talpoides), I examined the effects(s) of gravimetric soil moisture (i.e., 0%, 5%, 10%, 15%, 20%, 25%) upon soil-contact and –use behaviors. Six gophers received successive, 0.5 h/day exposures to one of the moist soils compared to dry (0%) soil in a 2-choice apparatus. Times in each compartment and observed …

Opportunities For Family Research In Marketing, Suraj Commuri, James W. Gentry

Department of Marketing: Faculty Publications

Family as a consuming and decision making unit is a central phenomenon in marketing and consumer behavior. However, in the recent past, there has been a decline in interest in family as a unit of analysis. Yet, at the same time, the family -- as an institution -- is undergoing a metamorphosis and currently stands at the threshold of significant transformation. In this paper, we argue that the decreased interest in family as a unit of analysis in marketing is largely due to the fact that many interesting propositions about family as a consuming unit remain outside the current perspective …

Women Managers And Gendered Values, Diane Kay Sloan, Kathleen J. Krone

Department of Communication Studies: Faculty Publications

In this study we interviewed 30 women managers to better understand ways in which they experience gendered values and behavior in organizational leadership and their responses to those experiences. The results, based on a constant comparison, thematic analysis, indicate the emergence of surprisingly strong and similar perceptions among the 30 women that there are distinct feminine and masculine power orientations in leadership communication with corresponding sets of gendered values: (a) open/closed and (b) supportive/intimidating. Their most common responses were: (a) rejection of masculine power, (b) self-doubt and blame, (c) competence, (d) confrontation, (e) isolation, and (f) resignation. These women judge …

Optical Communication Networks For The Next-Generation Internet, Arun K. Somani, Byrav Ramamurthy

School of Computing: Faculty Publications

Computer and telecommunication networks are changing the world dramatically and will continue to do so in the foreseeable future. The Internet, primarily based on packet switches, provides very flexible data services such as e-mail and access to the World Wide Web. The Internet is a variable-delay, variable- bandwidth network that provides no guarantee on quality of service (QoS) in its initial phase. New services are being added to the pure data delivery framework of yesterday. Such high demands on capacity could lead to a “bandwidth crunch” at the core wide-area network, resulting in degradation of service quality. Fortunately, technological innovations …

Using A Nitrate Specific Ion Electrode To Determine Stalk Nitrate–Nitrogen Concentration, Wallace Wilhelm, S.L. Arnold, James S. Schepers

Department of Agronomy and Horticulture: Faculty Publications

The end-of-season stalk NO₃ test has been used to determine N sufficiency in corn (Zea mays L.). Nitrate concentration is commonly determined with flow-injection analysis (FIA), which is accurate but uses hazardous chemicals and is time-consuming. Use of a simpler method of NO₃ determination, such as the NO₃ specific ion electrode (SIE), may save time and costs, and reduce hazards. The objective of this study was to compare estimates of stalk NO₃ concentration by FIA and NO₃ SIE. For FIA, NO₃ was extracted with 2 M KCl, and the extract was filtered before analysis. …

Deer Population Management Through Hunting In A Suburban Nature Area In Eastern Nebraska, Kurt C. Vercauteren, Scott E. Hygnstrom

United States Department of Agriculture Wildlife Services: Staff Publications

The Fontenelle Forest Nature Area (FF) maintained a hands-off management policy for 30 years until it was recognized that white-tailed deer (Odocoileus virginianus) populations had grown to such levels that they were severely degrading native plant communities. In 1995, members of a community task force decided to sponsor annual nine-day hunting seasons on FF after learning that densities exceeded 28 deer/km². Archers harvested 85 antlerless deer in the FF upland areas adjacent to residential Bellevue, Nebraska during 1996 to 1998. Muzzleloader hunters removed 53 antlerless deer from the FF lowland areas. Archery and muzzleloader hunters harvested …

Urban-Suburban Prairie Dog Management: Opportunities And Challenges, Gary W. Witmer, Kurt C. Vercauteren, Karen Manci, Donna Dees

United States Department of Agriculture Wildlife Services: Staff Publications

The historic range of the black-tailed prairie dog has undergone dramatic declines in the last century, prompting concern about the species' long-term viability. While considered a pest by many, others believe that the species is a "keystone" element of prairie ecosystems. Urban-suburban land managers are challenged with preserving colonies of prairie dogs on public lands while dealing with many conflicting interests, social costs, and risks. We review the management plans that municipalities have designed to reduce conflicts by using public input, zoned management, and a variety of management techniques. Areas of difficulty and research needs are also discussed.

Nanocomposite Copt:C Films For Extremely High-Density Recording, M. Yu, Yi Liu, A. Moser, D. Weller, David J. Sellmyer

David Sellmyer Publications

Nanocomposite CoPt:C films were investigated as potential media for extremely high-density recording. An annealing temperature of over 600 °C is necessary to form nanocomposite CoPt:C films consisting of C matrix and fct CoPt nanocrystallites with grain sizes of 8–20 nm and coercivities of 3–12 kOe. Coercivity and grain size increase with increasing annealing temperature and decreasing C concentration and they are insensitive to film thickness. The average activation volumes are about 0.9×10^-18 cm³. The properties of these nanocomposite CoPt:C films can be tailored to satisfy the thermal stability, coercivity, and media noise requirements for extremely high-density recording.

Blue Ribbon Commission Recommendations Executive Summary – November 1999, Lynn Cornwell

Range Beef Cow Symposium

Purpose: The NCBA Board approved and President George Swan appointed the Blue Ribbon Commission to create a stronger NCBA for today and tomorrow by establishing recommendations for:

• Commitment to the producer

• State-national partnership and membership

• Governance and representation

Convenience Beef Products: Trials And Tribulations In Development And Marketing, Jonathan Rocke

Range Beef Cow Symposium

The evolution of convenient beef products is not only an exciting phenomenon that is occurring within the beef industry but is an essential strategy and revolution that must take place if beef is to reverse its downward demand curve. It is not a matter of if, but when, not how, but how fast and certainly it is not a matter of telling the consumer how they will like their beef but rather listening as they tell us "How they would like their beef". It has begun and their will be no retreat.

Bacterial Diversity Within The Human Subgingival Crevice, Ian Kroes, Paul W. Lepp, David A. Relman

United States Department of Veterans Affairs: Publications

Molecular, sequence-based environmental surveys of microorganisms have revealed a large degree of previously uncharacterized diversity. However, nearly all studies of the human endogenous bacterial flora have relied on cultivation and biochemical characterization of the resident organisms. We used molecular methods to characterize the breadth of bacterial diversity within the human subgingival crevice by comparing 264 small subunit rDNA sequences from 21 clone libraries created with products amplified directly from subgingival plaque, with sequences obtained from bacteria that were cultivated from the same specimen, as well as with sequences available in public databases. The majority (52.5%) of the directly amplified 16S …

Investigating Ground And Surface Water In The Republican River Basin Of Nebraska Using Water Chemistry And Environmental Isotopes, Jennifer J. Schellpeper

School of Natural Resources: Dissertations, Theses, and Student Research

Recent legal battles concerning water use initiated an investigation of the stream/aquifer relationships in the Republican River Basin located in southwest Nebraska. Ground and surface water was analyzed for tritium, deuterium, ¹⁸O, and major ions. Temperature, pH, conductivity and alkalinity were determined on-site. Samples were collected during 1997 and 1998 from irrigation wells throughout the entire basin. Localized interactions between groundwater and surface water were examined during twenty-four hour pump tests performed on the irrigation wells screened in the alluvium along the river valley and its tributaries. These data show there are differences between the ground and surface waters …